LOCUS       NZ_CP048318             3174 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 32-4 plasmid p32-4_H, complete sequence.
ACCESSION   NZ_CP048318
VERSION     NZ_CP048318.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951925
            Assembly: GCF_010365345.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 32-4.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365345.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:42:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,109
            CDSs (total)                      :: 4,987
            Genes (coding)                    :: 4,642
            CDSs (with protein)               :: 4,642
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 345
            CDSs (without protein)            :: 345
            Pseudo Genes (ambiguous residues) :: 0 of 345
            Pseudo Genes (frameshifted)       :: 151 of 345
            Pseudo Genes (incomplete)         :: 215 of 345
            Pseudo Genes (internal stop)      :: 53 of 345
            Pseudo Genes (multiple problems)  :: 66 of 345
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048318.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="32-4"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p32-4_H"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            complement(361..789)
                     /locus_tag="GZS16_RS24750"
                     /old_locus_tag="GZS16_24750"
     CDS             complement(361..789)
                     /locus_tag="GZS16_RS24750"
                     /old_locus_tag="GZS16_24750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            947..1117
                     /locus_tag="GZS16_RS25355"
     CDS             947..1117
                     /locus_tag="GZS16_RS25355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001531327.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQARKIHN"
                     /name="hypothetical protein"
     gene            1081..1509
                     /locus_tag="GZS16_RS24755"
                     /old_locus_tag="GZS16_24755"
     CDS             1081..1509
                     /locus_tag="GZS16_RS24755"
                     /old_locus_tag="GZS16_24755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(2345..2539)
                     /locus_tag="GZS16_RS24765"
                     /old_locus_tag="GZS16_24765"
     CDS             complement(2345..2539)
                     /locus_tag="GZS16_RS24765"
                     /old_locus_tag="GZS16_24765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001485273.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2823..3146
                     /gene="mobA"
                     /locus_tag="GZS16_RS24770"
                     /old_locus_tag="GZS16_24770"
     CDS             2823..3146
                     /gene="mobA"
                     /locus_tag="GZS16_RS24770"
                     /old_locus_tag="GZS16_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_052584083.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQVIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          2575..2814
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(1785..2310)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.03"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          2824..3143
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="2.92e-61"
ORIGIN
        1 gacggtatga acaccgtaag ctcccaaagt gatcaccatt cgctttcatg catagctatg
       61 cagcgagctg aaaacgatcc tgacgcatcc ttcctgtttt tccggggtaa aacatctctt
      121 tttgcggtgt ctgcgtcaga atcgcgttca gcgcgtttca gtggtgcgta caattaaggg
      181 attatggtaa atatatgagc tatgcgataa ctttaactgt gaagcgatga acccattaca
      241 ggcaaagcca attactcctg acagtggttt agccagaagc agggctacca agaccaatgc
      301 aataagtaat atatcgtttt gctatcgtgc atcgctcgcg ctcagttcca ttgtgctttt
      361 ttaagctgtc gtttttctta cggtatatac cggtttttta tggcgtggtt tcttaacttg
      421 ttcagctact gatggaccca ttgtatctag gtagtcaact agctttgtta gatcataaat
      481 attgcgacca ccatatcggc gatcactctt cgatgttggt ttcttgtcca agagattagc
      541 tttttcaaga tcattgatag ctctctgaac agtccgtaca gaaaccccca tacgtatggc
      601 tagactttcc attgacggat gcggccactc ttgcaaactc caccagtgaa cgatcaggtt
      661 aagtagtgtg ttaaaggcca ctgaagttag ctttttctcg ttttgtataa aaaacaatac
      721 ggtaggcact gctgtccagc caagagacaa accgccagct ttccatttat tcttaacgga
      781 gtaagtcatt gattttccta agccccaaaa tatttaaagt atatattata tggtatattc
      841 atatgaatag ggtgacacta gcgccattat tgtgcaacca aaaaagacta ctctgaaaac
      901 gaggaaaaga ttttttcctg cctgaattag atacggagtt agcgatatga aaaccgaaca
      961 acgtcatgat cttgttaaag atattgaggt ttttggcgta tccttgtctc tgttgatttc
     1021 cagagcgaat gagaagtctg ttacaatgcc atctggtcta agtcgggagc agagaagagc
     1081 atgggcagcg gagcaggcgc gcaaaatcca caattgaata ttgtctcatt ctctgagacc
     1141 ttcaaccttt attacacatc cagatattct gcaaaaacac tcgataaaat cgatgatttc
     1201 attgagcatt ttgaaaaata caatctcttt ggcgatcctt taaaaggata tccagcttgg
     1261 actggcaaag tatcgccatc gtggaaagtg cctgatcatt acgaaaacaa agaagctatt
     1321 gagaagtatg ctagagctaa caaattatgg catgctcatt taggcgatcc ggtttttaaa
     1381 gatacgtttc atgggaaata caaggtttct gactgggtta ttcatttcca gcggctgaca
     1441 ccgaaccata taaagctttt agagcttgga taccatgacc caatgaagct acctcaaaac
     1501 tttgaatgat cgagcggcag gctaaatgaa atcttgagat tcattcagtc tcgtcgtaat
     1561 ctcactattg taaaaacgaa aaaaccaccc tggcaggtgg tttttcgaag gttagttaat
     1621 cctggcagat tctctaaccg tggtaacagt cttgtgcgag acatgtcacc aaatactgtc
     1681 ctttcagtgt agcctcagtt aggccgccac ttcaagaact ctcgttacat ctctcgcaca
     1741 tcctgcttac cagtggccgt tgccagtggc gttaagtcgt gtcttaccgg gttggactca
     1801 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1861 cccagcttgg agcgaacgac ctacaccgaa ccgagatacc aacagcgtga gctatgagaa
     1921 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1981 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2041 gggtttcgcc acctctgact tgagcgtcgg tttttgtgat gctcgtcagg ggggcggagc
     2101 ctatggaaaa acgccaacgg cgcggccttt gtccctggct tcggcttttt gctcacatgt
     2161 tctttccggc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     2221 acaccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaag
     2281 agcgccttat gtgacatttt ctccttacgc tcctctatgc cgttctgcat cctgtccgga
     2341 tgcgttatat cccggtaaga ttttccgctt caaagcgtgt ctgtatgctg ttctgaagtt
     2401 cttccgcgag ttcgtgcagc ttctcacaca tggcggcctg ttcgtcagca tcaagtgcgt
     2461 ccagtttttc gagcagcgtc aggctctggc tttttatgaa tcctgccatg ttgagtacgg
     2521 tttgctgctg cttgttcatc tttctgtttt ctccgttctt tctgtcatct gcgttgtgtg
     2581 attatatcgc gtaccacttt tcgactgttt taccgtcggt attctgctgt ctgactcttt
     2641 gacgggcatt tctgtcagac aacactgtca ctgccaaaaa actgccgtgc ctttgtcggt
     2701 aattcgaact tgctgacagg acaggatgtg taattgttat accgcgcata catgcacgct
     2761 attacaatta ccctggtcag ggctttgccc cgacacccat gtcagatacg gagccatgtt
     2821 ttatgacaaa acgaagtgga agtaatacgc gcaggcgggc tatcagtcgc cctgttcgtc
     2881 tgacggcaga agaagaccag gtaatcagaa aaagggctgc tgaatgcggc aagaccgttt
     2941 ccggtttttt acgggcggca gctctcggta agaaagttaa ctcactgact gatgatcggg
     3001 tactgaaaga agttatgaga ctgggggcgt tacagaaaaa actctttatc gacggcaagc
     3061 gtgtcgggga cagggagtat gcggaggtgc tgatcgctat tacggagtat caccgtgccc
     3121 tgttatccag gcttatggca gattagcttc ccggagagaa aactgtcgaa aact
//
