LOCUS       NZ_CP048316             4069 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 32-4 plasmid p32-4_F, complete sequence.
ACCESSION   NZ_CP048316
VERSION     NZ_CP048316.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13951925
            Assembly: GCF_010365345.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Environmental dissemination of Carbapenemase-producing
            Enterobacteriaceae in Switzerland
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Nuesch-Inderbinen,M., Stevens,M.J.A. and Stephan,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-JAN-2020) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 29-NOV-2019
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: 32-4.fa
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80x
            Sequencing Technology :: Oxford Nanopore/illumina minniseq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_010365345.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:42:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,109
            CDSs (total)                      :: 4,987
            Genes (coding)                    :: 4,642
            CDSs (with protein)               :: 4,642
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 345
            CDSs (without protein)            :: 345
            Pseudo Genes (ambiguous residues) :: 0 of 345
            Pseudo Genes (frameshifted)       :: 151 of 345
            Pseudo Genes (incomplete)         :: 215 of 345
            Pseudo Genes (internal stop)      :: 53 of 345
            Pseudo Genes (multiple problems)  :: 66 of 345
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048316.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="32-4"
                     /isolation_source="environment"
                     /db_xref="taxon:562"
                     /plasmid="p32-4_F"
                     /geo_loc_name="Switzerland"
                     /collection_date="2019"
     gene            complement(join(3206..4069,1..1296))
                     /gene="mobQ"
                     /locus_tag="GZS16_RS24710"
                     /old_locus_tag="GZS16_24710"
     CDS             complement(join(3206..4069,1..1296))
                     /gene="mobQ"
                     /locus_tag="GZS16_RS24710"
                     /old_locus_tag="GZS16_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_029976510.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAEHRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            1475..1729
                     /gene="mobC"
                     /locus_tag="GZS16_RS24715"
                     /old_locus_tag="GZS16_24715"
     CDS             1475..1729
                     /gene="mobC"
                     /locus_tag="GZS16_RS24715"
                     /old_locus_tag="GZS16_24715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            2261..2968
                     /locus_tag="GZS16_RS24720"
                     /old_locus_tag="GZS16_24720"
     CDS             2261..2968
                     /locus_tag="GZS16_RS24720"
                     /old_locus_tag="GZS16_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_024623342.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIQPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(939..1297)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1298..1474
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          2262..2968
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.859"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2..1296)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.306"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(3210..4069)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggcattgtca tttttcctga gctgttccgc tcgtcgctcc gcgcgactac gttctgtatc
       61 ctcctgctcc ttccggcgca attcctcttc acgtttaagc aactggactc tgagagctgc
      121 aaggccggat gccccccact gctgccactg tttttcaaat gccgtctgac tcgcgggaat
      181 gtcctgtttc tccaccttcc ggtaatgctg tcgcaatttt tccatttgct ggctcagcga
      241 ctggattttt ccctgttcgc tacggtattc cggtgccgcc cattgcatca ggggaatacg
      301 ggaaaaaccg gacgaaaacg gcgatgattt tttcaggctg gcagcatggc tcatcgcttc
      361 ctttcgttcg gtgttcagct cttcccagcg tctgcgcgtc gcctgcatat ctgccatcat
      421 cgacatggca ttactggtga gtttccatgc cgcattactg tccttcggca acgcttcgat
      481 gatgtcacgt agtgtgtacg aagttgcgtt gtcaggaagc acgatattca gaaggtcagc
      541 cacacggcgg gcaggctctg tcagcccgct gtttctgagt tcggtacggg aaatctgtat
      601 ttccaggtta agtctgttaa tcagacgatt ctgttcgccg cgttctgttt cgataccgcg
      661 tgtttccatc gcacaggcag actttccgag gtgaatacca ggcgttttct caagcccctg
      721 tcgctcgtat gaacgatggt ctatctcttc ctgataaccg gcgttcgcca gtgccctgtt
      781 cgcatggtca gcccatgatg cacgccacgt ctcggcatgt gtccggtcgt tccagtccct
      841 gacctttcca ccgaatccag caggtccgac agctctcgtg gtcagcatga tgtgcgcatg
      901 gggatttgtt ttatccatgt gatggaacgc cacatcagca atcatgccct gactgacaaa
      961 gttttctctg acaaaggcga gaacggtttc ccgtgcggca tcctgcggca gttcgcgggg
     1021 gatggcgagt tcaatttccc gcgcaagttg tgagttcttc cggtgttcgg ccttctcaac
     1081 ggcattccag agttcggcac ggttcgtaca ccaggcaggg gctgtcgcgg gagtcagaat
     1141 cacagcttca gccacaccgc ttttgcgggt gtagtcgtga gtcagtccgg tacgctcatc
     1201 aagcatcagt gaaccggaac gataggcagc agaagcaacg gcgctgtagc cgctgtttct
     1261 ggagatgatt ttcatgctga gatgataaat cgccatttct gccgtaatgc ctttttgtgg
     1321 ttttctttca gccccgcagg gcgcacacaa tcgaaacgga gtggagattg tataagtgcg
     1381 cacttcgtga ttttccgtgc attatgccag ctcagttttt aaactaccag tgacgcgcta
     1441 aaatgcgatt tttcatcgcg cacggagtta catcatgagc agaacactcg aacagaagat
     1501 tgctgatgct gaggccagat tacagcgcct gaaggcgaag agcaggagtc tggacacagc
     1561 gcagaaggtc attgtgggtg cagcattgct ggcaaaggtc agaaagccgg aggaggtgca
     1621 gttgcgtgcc tggttactcc agttcctgaa ggcagaggtg acacgacagg ctgatgtgac
     1681 gcgcatactg ccgctgatta acgagctgga agcgctgcca ggacagtgat tgtatcgtcc
     1741 gctcgccgca gtcccagcga ccgagcgtta gcgagcgagt gggcgaggaa gcggaagtga
     1801 atccgtcgcc tgattgagca catacgcacg gtttttcggg aggtgatatg cgctgaaccc
     1861 gcatgaatac tggatttcca cagggttgga gggggtgtat tcggaacttg caattctgct
     1921 tttttgcaac gcattgtttt tattggtttt ttgttttcag cactccaagt tatccacagg
     1981 ctcgtcgtta agtttttaga tcttgttttt aagatcttgt ttttaatact tgttttagcg
     2041 ccaaaattcc ttatagaaaa caaaatgtta caacgctata ggtgatatga tttaaggtaa
     2101 aggtgatata atttaaggtg cttgggtgag gtaatttaag gtaaaggtga ggagatttaa
     2161 ggtaatattc acaggaaaat ggggggtgaa aatataattg ctcaccaatg ctaaaggtga
     2221 taggttttta tttcacctca tttttggcct ggtggttaaa atgacatcaa accctctgat
     2281 agcctataag tccaatgcgc tggttgaggc cagttacaag ctaactttac aagaacagcg
     2341 tttcttattg ctctgcataa gccgacttaa ctccggtact gacgtagctt cacctgaatt
     2401 gcaaaagaca atgaccatta cagctgcgga atattttgat tcattccctg atatggggcg
     2461 gaaaaatgcc gaggttcagc tacaggaagc gatcgatcgt ttgtgggaca ggtcgatcat
     2521 tctcaaggac gacgagaagc gcgaggagtt ccgctggatt cagtatcggg cacagtacgc
     2581 gaggggagag gggaaagcac aaatcacttt ttctgacgcg gtaatgcctt atctgacaca
     2641 gttgaagggg caattcacca gagtcgtcat taaaaatatt tctaatttat caagaagtta
     2701 cagcataaga atatacgaaa ttctccagca attccgcagt actggggaac gtattatcgc
     2761 cctcgatgat tttaaatcat cactgatgct tgatggtaaa tataaagatt tcaaaacgtt
     2821 aaacagggat ctaatacaac cctgtgttga cgaactgaac aaaaaaagcg acctggctgt
     2881 aacggttgag acgatcaaaa aggggcgcac ggttgtagcc ctgcattttc gcttcaagga
     2941 agacaaacag atcaagatga cgatctagaa tcgattttaa ggcacctaaa acgctccctg
     3001 ctgcgttttt tttgtgttag tagtgcgaag gtactattga agtgaaaaat tgcgtcaaaa
     3061 gcgctctaat gcagcgaaaa aagaaccccc aatttttggg ggcggaaaaa caaaactatg
     3121 ctgtgtgtgc ttcttgcatt catccctgtc gatgaacatc gacgaacata acacgatgtt
     3181 aaacaaaaca aaatattgtt cacattcact gtgaccaacc gcgatcatgt ctcctctcgg
     3241 tggcgtgtgt tcgtgcatct tcctcgcgcc gccattcgtc ccaggtacga ttaaaaccca
     3301 gcgagcggaa cagatgatcc ggctgttttt ctggtgtgtt gacggcagca aaaagcggtg
     3361 cctcacgggt tttcagatga aactccataa gttccggcgc aggtgatatg tggctgtctg
     3421 catcgtgatt tacggcaaca ccataccctg cataccgtaa ccccttatcg gtcagtgcac
     3481 tgatgaccag ctggctgtta tcggctgagg gaacggcaaa cgcgggatac agcgtaccat
     3541 taagccggac aacaccgccc agagagtcat ccacgcccat gaccagcaaa agctgaagct
     3601 ggcgggtttc ccggtcgaca gaactcatta ccggcatcag cgtcggtagc agacgtccct
     3661 gcgtaaaatc caccttcctt gctgccagtg atggcatggc tggctggcgg tcttctccaa
     3721 gacagaaaga ctccggccat gtcttcggct ggcggagatt gcgggtatat tgcggccatg
     3781 tctggcaaac ctgcgtcaga agttgctcaa aggtcgcaga ggaataaaca cgttgcagtg
     3841 gtggatgttg accgatttcg cttcggtcac agatataacc actcctgtca cgcattaaat
     3901 caacacgctc aaagattctg gtgcggtatt tctccagctc gttcccccag agcgtgtatt
     3961 ctccgttccg gttgtgaagc agaaggtaac aggtcatttc cccgttacca ggcatggggg
     4021 cctcgccata ccccgtaacg actgcgctca gtacccctga atccataac
//
