LOCUS       NZ_CP048133             8731 bp    DNA     circular CON 25-FEB-2024
DEFINITION  Acinetobacter baumannii strain D46 plasmid pD46-2, complete
            sequence.
ACCESSION   NZ_CP048133
VERSION     NZ_CP048133.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13874778
            Assembly: GCF_018831125.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Hamidian,M., Cahill,S.M., Nigro,S.J., Judd,L.M., Kenyon,J.,
            Holt,K.E. and Hall,R.M.
  TITLE     Complete genome sequences of two sporadic Australian Acinetobacter
            baumannii ST25 isolates, D4 and D46
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Hamidian,M., Cahill,S.M., Nigro,S.J., Judd,L.M., Kenyon,J.,
            Holt,K.E. and Hall,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-FEB-2024) ithree institute, The University of Sydney,
            Harris Street, Ultimo, NSW 2007, Australia
  REMARK    Protein update by submitter
REFERENCE   3  (bases 1 to 8731)
  AUTHORS   Hamidian,M., Cahill,S.M., Nigro,S.J., Judd,L.M., Kenyon,J.,
            Holt,K.E. and Hall,R.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2020) ithree institute, The University of Sydney,
            Harris Street, Ultimo, NSW 2007, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2019
            Assembly Method                   :: Hybrid assembly v. 4.0
            Assembly Name                     :: D46-assembly
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/25/2024 08:35:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,165
            CDSs (total)                      :: 4,070
            Genes (coding)                    :: 3,979
            CDSs (with protein)               :: 3,979
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 35 of 91
            Pseudo Genes (incomplete)         :: 53 of 91
            Pseudo Genes (internal stop)      :: 13 of 91
            Pseudo Genes (multiple problems)  :: 8 of 91
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048133.1.
            Bacteria and source DNA available from Ruth Hall.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="D46"
                     /isolation_source="mid stream urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pD46-2"
                     /geo_loc_name="Australia: Sydney"
                     /lat_lon="33.87 S 151.21 E"
                     /collection_date="2010"
                     /collected_by="Peter Huntington"
     gene            139..1089
                     /gene="repM"
                     /locus_tag="KPZ59_RS19230"
                     /old_locus_tag="KPZ59_p200001"
     CDS             139..1089
                     /gene="repM"
                     /locus_tag="KPZ59_RS19230"
                     /old_locus_tag="KPZ59_p200001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            1082..1657
                     /locus_tag="KPZ59_RS19235"
                     /old_locus_tag="KPZ59_p200002"
     CDS             1082..1657
                     /locus_tag="KPZ59_RS19235"
                     /old_locus_tag="KPZ59_p200002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1677..1820
                     /locus_tag="KPZ59_RS19240"
                     /old_locus_tag="KPZ59_p200003"
     CDS             1677..1820
                     /locus_tag="KPZ59_RS19240"
                     /old_locus_tag="KPZ59_p200003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(2009..2527)
                     /locus_tag="KPZ59_RS19245"
                     /old_locus_tag="KPZ59_p200004"
     CDS             complement(2009..2527)
                     /locus_tag="KPZ59_RS19245"
                     /old_locus_tag="KPZ59_p200004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            2900..3187
                     /locus_tag="KPZ59_RS19250"
                     /old_locus_tag="KPZ59_p200005"
     CDS             2900..3187
                     /locus_tag="KPZ59_RS19250"
                     /old_locus_tag="KPZ59_p200005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            3174..3488
                     /locus_tag="KPZ59_RS19255"
                     /old_locus_tag="KPZ59_p200006"
     CDS             3174..3488
                     /locus_tag="KPZ59_RS19255"
                     /old_locus_tag="KPZ59_p200006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            3616..6027
                     /gene="znuD2"
                     /locus_tag="KPZ59_RS19260"
                     /old_locus_tag="KPZ59_p200007"
     CDS             3616..6027
                     /gene="znuD2"
                     /locus_tag="KPZ59_RS19260"
                     /old_locus_tag="KPZ59_p200007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            6332..6793
                     /locus_tag="KPZ59_RS19265"
                     /old_locus_tag="KPZ59_p200008"
     CDS             6332..6793
                     /locus_tag="KPZ59_RS19265"
                     /old_locus_tag="KPZ59_p200008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(7116..7289)
                     /locus_tag="KPZ59_RS19270"
                     /old_locus_tag="KPZ59_p200009"
     CDS             complement(7116..7289)
                     /locus_tag="KPZ59_RS19270"
                     /old_locus_tag="KPZ59_p200009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(7289..7660)
                     /locus_tag="KPZ59_RS19275"
                     /old_locus_tag="KPZ59_p200010"
     CDS             complement(7289..7660)
                     /locus_tag="KPZ59_RS19275"
                     /old_locus_tag="KPZ59_p200010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(7932..8186)
                     /locus_tag="KPZ59_RS20995"
                     /old_locus_tag="KPZ59_p200011"
     CDS             complement(7932..8186)
                     /locus_tag="KPZ59_RS20995"
                     /old_locus_tag="KPZ59_p200011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     Typing          140..1089
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtccacgtt taccttgcaa tatgtccacg tttaccttgc aatatgtcca cgtttacctt
       61 gcaatatgtc cacgtttacc ttgcattagt acacaaataa tattaacgtg tacttataca
      121 caataaaaaa tagtggctat gagagattta gttgtaaagg acaatgcctt aatcaacgca
      181 agctataact tagacttagt agaacaacgt ttaattttat tggctattgt tgaagcaagg
      241 gaaagtggga aagggattaa tgcaaatgat ccattagaag ttcatgcaga tagttatatc
      301 aatcaatttg gtgtacaccg taatacagct tatcaagcct taaaagatgc ttgtaaggat
      361 ttattcgcgc gtcaatttag ctatcaagag aaaaaagcta atgggaatat ccgaaatgtt
      421 atgagtcgtt gggtatctca aattgcttat aacgacaatg aagcaactgt agacttaata
      481 tttgcacctg ctgttgttcc cttcataacc cgactggaag aacaatttac taaatatgaa
      541 ttacagcaag ttagtagtct tagtagcgct tatgccattc gcttatatga gcttctaatt
      601 cagtggcgaa gcactggtaa aaccccaacc atagaactac aagaatttag aaagaagtta
      661 ggcgttcttg ataatgaata tttacggatg gctcatttaa aagagcgtgt tttagagctt
      721 tcaattaaac aaataaatga gcatacggat ataactgtaa aatatgaaca gcataaaaga
      781 ggacgttcta tttcaggatt ttcttttacc tttaaacaga agaagaagga tagcccatca
      841 atagaaagag atccgaacac tttggagctt ttttcaaaga tgaccgatgc tcaacggcat
      901 atgtttgcaa ataaactttc agaactccct gaaatgggtc gctattcaca aggaacagaa
      961 agctaccaac agtttgctgt acgtattgct gagatgctac aagatcccgc tcaattcaaa
     1021 gagctatacc catacctaaa aaaagtggga tacatgccat caaataaaaa ggacaccgta
     1081 aatggctaag ttatcactaa gtgaagtatc taaaaaattt catgtggata gatcaaccat
     1141 ttacagagct gtacgtaatg gacgtttatc acgctccagt gatggacaat tcgatctagc
     1201 agaggtcata cgatgctttg gagaacctga gcaaacatct caaaaaattg aatcatctaa
     1261 gcaagaaggt gatgaatcta caaaaaaact tattgcccat ttagaaaatg aagtcaaaaa
     1321 ataccaagaa cgtgaagaac ggttaatgca acaaattgac cgtatgcaaa cactcattga
     1381 gctgaaaagt gttgcacctg ccacagcagc accacaccaa gatgctacgg catgcgacac
     1441 caagatgcca cagcatgcga caacacaaca agacactgat aacaaaaaga ataatgaatt
     1501 aaatattgca gaaaatgtag cagtgccaca gcaagaaact acggcatacc acacccaaac
     1561 gctacagcat gccacgttgc aaagtgtggc agtgccacaa cacaaaaaac gtggtttatt
     1621 tggccgtgtg ctgaatgccg tctttgataa tgactgagca ataagggtga aagatcatgc
     1681 cgaaactgaa agacattgcc ctgggaatta ttgtagctcc gctgctgatc ccgatcatgc
     1741 tgattgcatc gtaccaggat aaaaaggcgc tgaagaaaga gctggatgaa tgccaaaaag
     1801 aaaaagacca ggcatcctga ttattcaatt gagctaatca aaagtgtgga acattgatga
     1861 aataatagcg tggaacgtga aaagtcataa aaaaagcccg aacagggatg ctcgggctat
     1921 aaactagaaa ctacagcgtt gaattataag gataattata cagcatttcg tataaggtgt
     1981 attatgttaa ttttaggaaa tattaaaact aattttcaga cttatcccac caactttgag
     2041 ctaaatcagt atttttagga atacctatac catcacgata cataacggaa agacttcgca
     2101 ttgctttctt attacctaac tttgcagcct tttcacgaag ttcaaaagct ttattaatat
     2161 taacttctat tccgctatct gcacgagtat atagatcagc taactcaacc attgattcag
     2221 aatggccatt ttctgaggct ttctgaaacc acttaaaagc tttttggaaa tgttctaaag
     2281 aatcgttttt gtcatcatct tccattaatc tagcataagt aacatatcca tcagctaacc
     2341 aaaacattgc tgctacattt ccattttctg ctgatttaag agcccaaact tcagcttgat
     2401 tttcatctga atcaacacca tgaccatata gataaatctc ggctaattct aactgagcag
     2461 aagtatctcc attttctgct ttcgctaata aatcattaga aaaaacaggt tctgcaaatg
     2521 tttccattga aaggcaaact cctaaagcca taaaaatatt tgttaattta ttcaattttt
     2581 aacctttaaa ctaaactgtt aggcttgtat atttttatat catacctaaa agaaaactct
     2641 aaaattagag tatgtcttat ctctatttaa cataatggcg attatacgaa gtacacttgt
     2701 atattaatat aaatatcaac tacttaataa aaatagaaaa atcccaaaat gcccaaaaag
     2761 ccgactttga aacaagtcgg cttttttatg agcagttttg atacttcttg ccaataattt
     2821 tgactaaaaa ttacacgaat ctggttgagt gatacaattt tgtgatacat tattgtatat
     2881 acaaatttga atgtttttta tggaacagta ttttgaatgg gatgaggcta agaatcgaaa
     2941 gaatcagaaa aagcatgata tctctttcga aactgcaagc cttgtttttg aagatccatt
     3001 acggatctca atccaagaca gacataccaa tggtgaagaa cgttggcaaa ccattggacg
     3061 agttaaaggc gtactaatgt tattggtggc tcacaccatt tttgatgaag atgactgtga
     3121 aatcatccga atcattagtg caaggcaagt cactaaagcg gagcgaaaca aatatgagca
     3181 tggttagata ctcacacaaa gagctgaatg agaaatttgg tgagaagcaa gatgctgaaa
     3241 ttcaacgctt gcttgctaaa ggcacggttc ctgatgatca gctagatcta tcagatattc
     3301 ctgaaattac agactggagc aatgctgtac gccaaaatca attttaccgc ccagtaaagc
     3361 aacaaacttc aattcggtta gatgctgatg tacttgcttg gttcaaagca caaggcaaag
     3421 gttatcaaac acgtatgaat gaaattttac gagatgctat gctaaaagaa ttaaaaaatc
     3481 atcaataaga atgggagctt aggctcccat ttttgacttc atttttaggg gggattacca
     3541 ttcaaaaatt taaatagtag attttataaa tttttaagtg ttatattata atataacatt
     3601 aaatttggtt gttttatgct aaataaaagt aaactatttt tagctttaat tactttaggg
     3661 gcctcgaaaa ttttattggc agctgaaggg cctgttacta ccttaaatac aatagtatta
     3721 actgcacaat ctgatgagtt agggtctgag ctgttaggca aaagtctaaa tgttagtaat
     3781 cagtttattg atactagtaa acttaaacag cgttcgacaa ctctagggga tgccttgggt
     3841 acggaattag gaattcattc taatcaatat ggaggtggag catctactcc tattattcgt
     3901 gggcaggaag gaaaacgtat taaagtttta cagaataatg ctgacgtgtt ggatatgtct
     3961 aatatgtccc cagatcacgc tgtcacagtt gaaccaagtt tagctaagtc tattgaaatt
     4021 atacgtggtg caagtaccct tttgtatagt tcaaatagtg cggcaggggt agtcaatgtc
     4081 attgactata aaattccaac ccaaatgcct caagacggat tagagggtaa tactacatta
     4141 cgttttaata ctggcagtaa tgagaagttg accacagcag gtgtaacagt tggcttaagc
     4201 ccacatgtag ctttacgggc agaagggtta tatcgtaatg ctggtaacta taaaactcct
     4261 cattaccagt ctagctctta taattcttta gaagatcttg aaaaccaaaa cagtatttat
     4321 aaaaatttaa aatatttacc agaaagctgg gcagagtcga gagtaggtac gcttggatta
     4381 tcctggatag acgataatac ttatttagga gtttcttata cacatcgtca tgatgaatat
     4441 ggtttaccag cccatagcca tttatatgaa ggttgtggtg cctctgcaat cggtatcgat
     4501 tcacgaatta gtggattaaa aaattatctt ttatattatc ctcaattgat ggatgagcaa
     4561 gatattaatt acataaatcc acgtcctgat tgtcatcagc ataatcatat tcatgaaact
     4621 aatttctctc ataatgctcc ttatattgat ttaaatactc gacgttatga tgtgcgtgga
     4681 gaattcactc aaccatttac cggtattgac aaaattcgta cctcattgag ttatatagat
     4741 tattttcaca atgaactcga gggagataag attactaact tttttaaaaa tacaggaaaa
     4801 gtaggccgca tagagttatc acatcagcca ttaggggaat taaccggtat cttggggcta
     4861 caatatttgg aacaggataa tagtgcttta tcaccagtcc attctcaaga aggccatacc
     4921 acctatctag ataatcagca gctattaaat cgaaatgtca ctaaaaactt tagtgtattt
     4981 ggcctagaaa aatataactg gaacgacttt actttcgaac taggtgctcg tatagaaaag
     5041 cagaaagtta gtatggatta tgatatagaa aaaattaaag attcaatgaa accttggcct
     5101 aataaatata acagtcctta tgtagaaaaa aataataaaa tcagggctca aaatttaaaa
     5161 tctattttag aagctgttca acctaacaaa gaaacagctt tttcttatgc aggtacagtc
     5221 cactggagat ttgctccaaa ttatatctta tcattaacag gtacacatca agaaagatta
     5281 cctaatgcac aagaaatgta tactcatggt atgcatctcg ctactaattc ttttgaaata
     5341 ggtaaccgct ttctaagaaa agagaaatcc aataatttag aaatcagtct ggcctataag
     5401 gatgatttac ttgattatca aatcagtact tactattatg attttgataa ctatatatat
     5461 ttacagacac tcaatgaagt tttaggaact acaaaagtta gagatcagca tacactacgt
     5521 attaatcatt atagccagtc tgctgctaat ttttatggat tagagggcaa catagggtat
     5581 caatttaatt ctgtttatca cggctctctt tttggagatt atgtgaaagg ccgtttaaca
     5641 aatttacctg atgctgtaat tgcatacgat atctggaata gagaaccgac tttagctccc
     5701 caaaaggatc gttacactcc tagattacct cctgctcgtt tgggtacacg actaaaagct
     5761 gattttgatg aatcgcttaa aggtgaaata gaatattatc gagtctttaa acaagataat
     5821 atttctaaat ttgagcaggt tacatcaggt tataatatgc ttaatatgac tttagcatat
     5881 aaaaataagc tatctcacac agaatatgat ttattcttta aagcgaataa tttacttgat
     5941 cagaaagtct atgcccatga aacatttttg ccatatattc cccaaattgg aagaaatttt
     6001 agtttaggct taaatcttaa tttctagata agatactgta atcttctaag aaaatttctt
     6061 agaagatttt tcttataaaa tgattagatt aataattaag gtaagcgtcc gctgaatccc
     6121 atacctattt tttaattcga tttaggtttc cagcagtgtg gcagtaactc ttcaatctgg
     6181 gtcactttat gtgtcggcag ccttttcagc actgaatagc caccgatttt gtagacacct
     6241 tttagctaga taaaaagcaa tttttttgat ctttaaagaa tgaggatata agctgtttaa
     6301 aacttaattc taattggtta ataggtgaaa aatgaaatta gcagaagctc tcttacttcg
     6361 aagtgatcaa caaaaaaaaa ttatttcact aaaacaacgt atcaatgcaa atgtattggt
     6421 acaagatggt gatcaaccat ctgaagatcc aaatgaacta ctaaaacaag tattttcttt
     6481 aattcaagaa tttcaaaaat taagttatgc aattcatgaa actaatgctt taacaaaatt
     6541 aaatgatggc cgttctctac ttgctttatt gactttgcgt gatgaatttg tagaacagca
     6601 caaaacccta actgcagcaa taagtaatac atctcgtgaa tctgatcgtt atagtacgcg
     6661 ggaaataaag tggcataaag ttattccagt ttcatcatta caaaaacaag ctgatgatat
     6721 tagtcttaaa ttacgtgatt taaacgtgtt aattcagtcg actaattgga aaattgattt
     6781 gattgaagcc taatagtcct ttagatataa tcaagttgaa catactgggc gagtacaaga
     6841 tcccttactt ccacaacata ggattgtaaa taaagtgtag tggcttgcaa agcaagtgga
     6901 taagcttaaa attttaagta caaatcaatc catcacttgg cataactcaa atctttgtgc
     6961 aaacaaagtt acacgttact tataactacc atgtactgac agtatgttca cctcaattac
     7021 taaaaataaa gaaatgaaca agcatcagct caactacatt tctattttta attcagttcg
     7081 gcatttaaca taaaatctct tatacgaagc ccaggttaat tctgggcttt ttgttcaatc
     7141 tcatcattgg ccgagaacat acttggattc gtaagcctga tattttcctg acaacttcca
     7201 cacagcatca atcctgcaaa aagttgtaca ttttgtctat tttcacaaag cgtacatggc
     7261 tttgaaagct gaataatatt atctgacact actttaccct cttatagtta tcatctgcat
     7321 atgaataacc tttagcttgt atcattctaa cctcatcttt agaagcgcca tttttcatta
     7381 agagccacaa catcatgaaa tatagtccag aaaagtcttc atagtatttt tcagaaaaat
     7441 tgttatcttc ctcaaaatca tctattcgct tttctaatcg attcaccaga tcctcaatac
     7501 gatcggcttg ctgtttaaca gcaatagatt gaaaatcatt ggtcaaaatt ttactgaata
     7561 atttactggt tctgattcct tctgaattag ccaattgatc gtagaattct gctacttgtg
     7621 gatctaacct gactgcataa gttttgactt tttcgcccat aaattacacc tcttattaaa
     7681 attagatttt gtacaaaatt aaatatatat aattcaacta attaaataaa aaataataat
     7741 tagattacgt acaaacttat ttgtacagaa ttaatttatt ctaaaacaaa aacttattta
     7801 aatataatgc tttttttatg tacaatcatg agtacatatt ttttcttaaa tttatcaata
     7861 tcttatctaa aaaaagtgtt gttttgatgt acaaccatga gcacagcata aaaaatagat
     7921 aattcaatca cttattctaa aaaaagacca tttttctgta cacgtttctg agaccctaaa
     7981 aaatcgctga aatccttatc ctgtaaggct ttgaggggtg tcatcgttag atgcgtggtg
     8041 tgtgacaaaa aaatcccggc atgtgccgga tcttggattg gaaaattggc taaagtgacg
     8101 tagggccggt gcttggtttt gcatggaaaa aagtatttat ttccttgttt agaaaaacgt
     8161 aaaaaatcaa ttttttgtcc attcatccag gttaaaaatt tcaacccaaa actttaatta
     8221 tgaaaagctt catagaacgc gttcaaatgc gatttaagag cctttaccta gaaaacatag
     8281 atctcatagt gaaaaacaca aaacagctca aaaaacgcaa atgagagcga aataaagagg
     8341 tattttgact ttagatagat gcatagagcg agtgtctacg agcgaactat caaaatttgc
     8401 gcctagaccc tctgaaaaac atttttttgt cctctttagc ctaagaaagc gtaagtttcg
     8461 tgcagaaatt tgctcctgga tcgagcgtag cgagtaaaaa agttttatga gcgaagcgaa
     8521 ttccaagttt cttttgcttt tttttaaagt cacgccagta ttaaccaaaa aattgcccca
     8581 acgaactgag cgaaagcgaa gttcaataga gtttgagcga agcgaaaacc aagggcaatt
     8641 tttcattccc tgggctttta attattttaa agtttttaaa tacttttaaa tggcttgaaa
     8701 gccttatgaa taaagggttt tagctcttat a
//
