LOCUS       NZ_CP048013             2088 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain RUT3575 plasmid p3575-5, complete sequence.
ACCESSION   NZ_CP048013
VERSION     NZ_CP048013.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13031455
            Assembly: GCF_016803855.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Moreno-Mingorance,A., Gonzalez-Lopez,J.J. and Fabrega,A.
  TITLE     First report of an Escherichia coli ST410 co-producing NDM-5 and
            OXA-181 in Spain: dissemination from Pakistan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Moreno-Mingorance,A., Gonzalez-Lopez,J.J. and Fabrega,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2019) Microbiology, Hospital Universitari Vall
            d'Hebron, Pg Vall d'Hebron 119-129, Barcelona 08035, Spain
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. Unicycler v0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 64x
            Sequencing Technology :: Illumina Miseq andOxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 03:51:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,875
            CDSs (total)                      :: 4,758
            Genes (coding)                    :: 4,502
            CDSs (with protein)               :: 4,502
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 256
            CDSs (without protein)            :: 256
            Pseudo Genes (ambiguous residues) :: 0 of 256
            Pseudo Genes (frameshifted)       :: 84 of 256
            Pseudo Genes (incomplete)         :: 162 of 256
            Pseudo Genes (internal stop)      :: 50 of 256
            Pseudo Genes (multiple problems)  :: 35 of 256
            Pseudo Genes (short protein)      :: 4 of 256
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP048013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="RUT3575"
                     /isolation_source="Rectal swab of patient hospitalized for
                     an unrelated disease"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p3575-5"
                     /geo_loc_name="Spain: Barcelona"
                     /lat_lon="41.43 N 2.14 E"
                     /collection_date="2018-11"
                     /collected_by="Vall d'Hebron University Hospital"
     gene            join(1128..2088,1..5)
                     /locus_tag="GW576_RS24070"
                     /old_locus_tag="GW576_24065"
     CDS             join(1128..2088,1..5)
                     /locus_tag="GW576_RS24070"
                     /old_locus_tag="GW576_24065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039064478.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLAIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            9..275
                     /locus_tag="GW576_RS24075"
                     /old_locus_tag="GW576_24070"
     CDS             9..275
                     /locus_tag="GW576_RS24075"
                     /old_locus_tag="GW576_24070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          1522..1753
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 attaacgtat gaatattaat attgaatacc tgaatggaaa taagactatt ggtttatttt
       61 ttttaagaag tgaagcggtg attcctgaca ggtttaaaaa ccttattttg cttattgatg
      121 gattaagttt tggcacattt ggttttcatc cgcacgaagg ttttgaggat gaattaattt
      181 tatatattca gaaaacaaac gagagggtaa aaactctttt tgtgaaaata gatttaaacg
      241 gtatcaagtt tggattttta agaacgcatt cttagttctg gaaaagagcc agcggcaggc
      301 tgaggtgata ggtacgagat tgcatgcaat ctctagtgct ctgtctatcc tgcattatcc
      361 tcagcattat cctcagcctt gccaactcga caccaatgca ggatagacaa tccgatgtca
      421 aatgttaaca ctctgcgagt ggtacatttt ccccggatta tcgtcctgag cctgccgctg
      481 gctctctttc taccgcctcg ctttgctcgt tgctcaacgc ctcacagaca cggattaaaa
      541 tccgcatccg ttcaccgttt tttaaagtcc gttaaaagca tgatgccatc tccgagagtt
      601 aatctcgtca aatgctaaat cgtgggggtc ccctttgggg ttccgattta gtgattgacg
      661 acaccaccga ttaaaaaact tatgcggggt ggatggtttc acgaagtgag gccatccacc
      721 tgtaagacag ggttttgttt ttatcccctg ttttggtgat cgggtgtgtg gaaaaggttg
      781 gggtaagccg ttcggggtgc ttgttttggg gggttaaaat tgtggttatt ttttgcgcaa
      841 ttctcgcgcg tgatccttgt atttatactt aagggataaa tggcgggtat gaaatagtgg
      901 tttagcccag taatgacgag gctttgagtg ggttttgaca ggtcaaagaa aatggagcag
      961 aattgaggcg tttttaatcg gcgttgggga gtgcgtcaac actccccaac atttcgaatg
     1021 tgtcacctca gcggcaaact ctggtgacat gtactggctc gcaatgcaca ggtacgtgat
     1081 gaatatacca catcaaatca cagcctgccc agatcggagc aggcttaatg tcagaagata
     1141 aattcctttc ggactacagc ccccgtgatg cagtttggga tacccagcgc acgcttaccg
     1201 attctgtcgg gggtatctac cagactgctg ctgaattcga gcgctatgca ctccgtatgg
     1261 cctcctgtag cggtttgtta cgttttggtt ggtctaccat catggaaacc ggagaaacgc
     1321 gcctacggct tcgtagtgcg caattttgcc gtgtccgtca ttgccctgtc tgccagtgga
     1381 gaagaaccct catgtggcaa gcccgttttt atcaggctct accgaaaatc gttgtggatt
     1441 acccgtcttc ccgatggttg tttctgacgt taactgtcag gaactgcgag ataggtgaac
     1501 ttggaacagt ccttacagca atgaatgcgg cgtttaagcg aatggaaaag cgaaaggagc
     1561 tatcacctgt tcaggggtgg atcagggcta cggaggtgac gcgaggtaag gatggcagcg
     1621 cacatccgca ttttcactgt ctgctgatgg tgcaaccttc ttggtttaaa gggaagaact
     1681 acgttaagca cgaacgttgg gtagaactct ggcgcgattg cttgcgggtg aactatgagc
     1741 cgaatatcga tattcgggca gtaaaaacta agacaggtga ggttgtggcc aacgttgccg
     1801 agcaactgca aagcgcggtt gctgaaacgc tgaaatactc cgttaaaccg gaagatatgg
     1861 caaacgatcc tgagtggttt cttgagctga cgcggcagct tcacaagcgc cgttttatct
     1921 cgaccggtgg agcgctaaaa aacgtcctcc agttggatcg agaaaccaat gaggatcttg
     1981 ccattgccga cgatgtaggg gatggcactg atgacgggaa gcggacggcg tttgtctggg
     2041 attcaggtaa acggcgttac aaacgcgccc ctgagaagga taaatcgg
//
