LOCUS       NZ_CP047846            20651 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Staphylococcus aureus strain UP_591 plasmid unnamed, complete
            sequence.
ACCESSION   NZ_CP047846
VERSION     NZ_CP047846.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11175610
            Assembly: GCF_009912875.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20651)
  AUTHORS   Dilthey,A., Meyer,S. and Kaasch,A.
  TITLE     Increasing the efficiency of long-read sequencing for hybrid
            assembly with k-mer-based multiplexing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20651)
  AUTHORS   Dilthey,A., Meyer,S. and Kaasch,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2020) Institute for Medical Microbiology and
            Hospital Hygene, University Hospital Duesseldorf, Moorenstrasse 5,
            Duesseldorf 40225, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler Hybrid Assembly v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 43x / 446x
            Sequencing Technology :: Illumina MiSeq / Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:22:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,706
            CDSs (total)                      :: 2,625
            Genes (coding)                    :: 2,552
            CDSs (with protein)               :: 2,552
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 48 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 12 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047846.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="UP_591"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed"
     gene            166..842
                     /locus_tag="E3S82_RS13545"
                     /pseudo=""
     CDS             166..842
                     /locus_tag="E3S82_RS13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            <853..1135
                     /locus_tag="E3S82_RS13635"
                     /old_locus_tag="E3S82_13380"
                     /pseudo=""
     CDS             <853..1135
                     /locus_tag="E3S82_RS13635"
                     /old_locus_tag="E3S82_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            2343..2960
                     /gene="cadD"
                     /locus_tag="E3S82_RS13385"
                     /old_locus_tag="E3S82_13385"
     ARG             2344..2957
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            2979..3326
                     /locus_tag="E3S82_RS13390"
                     /old_locus_tag="E3S82_13390"
     CDS             2979..3326
                     /locus_tag="E3S82_RS13390"
                     /old_locus_tag="E3S82_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119691.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            3495..3587
                     /locus_tag="E3S82_RS13395"
                     /old_locus_tag="E3S82_13395"
     CDS             3495..3587
                     /locus_tag="E3S82_RS13395"
                     /old_locus_tag="E3S82_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3799..4773
                     /locus_tag="E3S82_RS13400"
                     /old_locus_tag="E3S82_13400"
     CDS             3799..4773
                     /locus_tag="E3S82_RS13400"
                     /old_locus_tag="E3S82_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WP_000072390.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSGWI
                     RVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN
                     YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL
                     LQKNYKATLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
                     /name="DUF4062 domain-containing protein"
     gene            complement(4893..5708)
                     /locus_tag="E3S82_RS13405"
                     /old_locus_tag="E3S82_13405"
     CDS             complement(4893..5708)
                     /locus_tag="E3S82_RS13405"
                     /old_locus_tag="E3S82_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000164958.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(5701..7144)
                     /locus_tag="E3S82_RS13410"
                     /old_locus_tag="E3S82_13410"
                     /pseudo=""
     CDS             complement(5701..7144)
                     /locus_tag="E3S82_RS13410"
                     /old_locus_tag="E3S82_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            complement(7116..7709)
                     /locus_tag="E3S82_RS13415"
                     /old_locus_tag="E3S82_13415"
     CDS             complement(7116..7709)
                     /locus_tag="E3S82_RS13415"
                     /old_locus_tag="E3S82_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690638.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            complement(7973..8353)
                     /gene="blaI"
                     /locus_tag="E3S82_RS13420"
                     /old_locus_tag="E3S82_13420"
     ARG             complement(7977..8353)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(8343..10100)
                     /gene="blaR1"
                     /locus_tag="E3S82_RS13425"
                     /old_locus_tag="E3S82_13425"
     ARG             complement(8347..10100)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.56"
                     /coverage="100.0"
     gene            10207..11052
                     /gene="blaZ"
                     /locus_tag="E3S82_RS13430"
                     /old_locus_tag="E3S82_13430"
     ARG             10208..11049
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            11421..11953
                     /locus_tag="E3S82_RS13435"
                     /old_locus_tag="E3S82_13435"
                     /pseudo=""
     CDS             11421..11953
                     /locus_tag="E3S82_RS13435"
                     /old_locus_tag="E3S82_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436987.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /name="recombinase family protein"
     gene            12367..12660
                     /locus_tag="E3S82_RS13440"
                     /old_locus_tag="E3S82_13440"
     CDS             12367..12660
                     /locus_tag="E3S82_RS13440"
                     /old_locus_tag="E3S82_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000726502.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            12735..14669
                     /locus_tag="E3S82_RS13445"
                     /old_locus_tag="E3S82_13445"
     CDS             12735..14669
                     /locus_tag="E3S82_RS13445"
                     /old_locus_tag="E3S82_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000713729.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS
                     DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI
                     FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL
                     IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI
                     FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEK
                     QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD
                     FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD
                     RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS
                     GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH
                     GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK
                     YFKDLMREI"
                     /name="bacteriocin-associated integral membrane family
                     protein"
     gene            14673..14984
                     /locus_tag="E3S82_RS13450"
                     /old_locus_tag="E3S82_13450"
     CDS             14673..14984
                     /locus_tag="E3S82_RS13450"
                     /old_locus_tag="E3S82_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WP_000831610.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFLK"
                     /name="YxeA family protein"
     gene            14996..15622
                     /locus_tag="E3S82_RS13455"
                     /old_locus_tag="E3S82_13455"
     CDS             14996..15622
                     /locus_tag="E3S82_RS13455"
                     /old_locus_tag="E3S82_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000593103.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            15860..16489
                     /gene="mobV"
                     /locus_tag="E3S82_RS13640"
                     /pseudo=""
     CDS             15860..16489
                     /gene="mobV"
                     /locus_tag="E3S82_RS13640"
                     /inference="COORDINATES: protein motif:HMM:NF013258.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            16583..17128
                     /locus_tag="E3S82_RS13470"
                     /old_locus_tag="E3S82_13470"
     CDS             16583..17128
                     /locus_tag="E3S82_RS13470"
                     /old_locus_tag="E3S82_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000273005.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMILD
                     DYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKE
                     LNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQ
                     AQEREIIFKEDREDGYTL"
                     /name="hypothetical protein"
     gene            17512..18372
                     /locus_tag="E3S82_RS13480"
                     /old_locus_tag="E3S82_13480"
     CDS             17512..18372
                     /locus_tag="E3S82_RS13480"
                     /old_locus_tag="E3S82_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000145511.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQL
                     ELNWE"
                     /name="replication initiation protein"
     gene            18459..18800
                     /locus_tag="E3S82_RS13485"
                     /old_locus_tag="E3S82_13485"
     CDS             18459..18800
                     /locus_tag="E3S82_RS13485"
                     /old_locus_tag="E3S82_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000761341.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(19039..19110)
                     /locus_tag="E3S82_RS13490"
                     /old_locus_tag="E3S82_13490"
     CDS             complement(19039..19110)
                     /locus_tag="E3S82_RS13490"
                     /old_locus_tag="E3S82_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106873653.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            19259..19444
                     /locus_tag="E3S82_RS13495"
                     /old_locus_tag="E3S82_13495"
     CDS             19259..19444
                     /locus_tag="E3S82_RS13495"
                     /old_locus_tag="E3S82_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000466619.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(19663..20406)
                     /locus_tag="E3S82_RS13500"
                     /old_locus_tag="E3S82_13500"
     CDS             complement(19663..20406)
                     /locus_tag="E3S82_RS13500"
                     /old_locus_tag="E3S82_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843757.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR
                     QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND
                     NDSDIEEKSEETEAKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     ARG             10208..11052
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     BGC             7367..17660
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1193..1340
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="2.96e-25"
     Typing          17513..18372
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19664..20406)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          15861..16135
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 ttggtaataa aacgtttaga aactgtttat ttatataatt ttcgatactt agattatata
       61 atgaataata tcttagtgca agcacctaga ttattaaata aacacaaaaa atgtaagcgt
      121 tttattacct cttaccaaac taaaattcgg aaagaggttt ttattatgtc aattaaactt
      181 tacaacataa atgaggaata cagagaaaag ttttatcaaa taccgaaagt attttttaca
      241 aatcctaaat atatcaagtt atccaatgat gcaaaaatgg catggggtat tcttagagat
      301 cgtttagatt tatcaattaa aaatggttgg gttgattcta aaacaggtaa tatttatttt
      361 tattacaaaa atgaaaaccc acaaaatata ttaaattgca aaaaagataa agtaattaaa
      421 ataaagaaag aacttaattg aagccgaatt actactacaa gaaagacaag gtttaaataa
      481 gacaaataaa ctttatttag ttaaaccaga agtaacagaa gatgatattt ataaaattca
      541 aaagcaagaa aacactgttg agagcagcaa tggcaaggaa gtcggaaaaa ccgacttcaa
      601 gaagtcgata aaacagaatc taatgatact gaacttagct atactgaatc taacaatact
      661 gaaataaatg atatgaatga cttgaatgat atagagtccg aaaatgaaat tagtaatcat
      721 tcaagtcaat gcaaggttat ttaatgcaaa caattttaac tgaatttgaa gaaacacatg
      781 cactttatat gcgccgtaaa aacatgaaac aatataatat ctttaatcaa tcattcaaat
      841 gattttataa caatcaaaga tacatcaaaa tatttcgtta aatcatctga ctttttaact
      901 gatagcgatg aaaaaatcaa gaaattgtca gtgatctaga aaaagatttg tatcgaaaac
      961 gtatgttaag ttatggtgga ttgcttaaat aaaaacataa aattttaaac ttagacgatg
     1021 ctgaagatgg caatttaatt aatacaagtg atgaagataa aatagcaaat gaagaagaaa
     1081 aggcacattc gattacggca attttgaaaa gcaaaattat tatttaaggc attagtgata
     1141 actgatgtct ttttttatta cttttttgta aaaaaagtgc tgtcttattt ttgtgacaaa
     1201 tgctgtatgt agtgtcacaa aagtgtgaca cttgagcttt ttatgacccc acacaaaaac
     1261 attttcgaag ccttgaaata tctggctttg ccagatcccc cattcattga tggggacaaa
     1321 tttcccttat gctcttacgg agattttaga gttaaattaa aatttctaag tttttgataa
     1381 gaaaagccct gcaggaattt agaagaacgt ggggaagttt taaagaattt tatgctaatt
     1441 tttaatttgc atgtaaatgg gcagtgtcta aaaaatagtt attgtttttg ctcaaatttt
     1501 tgtttttgaa aatagcatat atttatttgg ctcatatttg cgttttaaaa gcgtatataa
     1561 gttttagata aaactatatg aatcacccct aaatctttaa aatgccctct taaaattcaa
     1621 aataagggca taaaaaataa aagcccccca gataaagtga ggtagctttt tctttcgacg
     1681 ccgtatcgtc gatgtaattc aaaaaagtta tgggctataa atctacatca ctttttcaag
     1741 aatgtagtag caatattcaa cgcaaattaa tttttataac tgctcggaat atctcaagcc
     1801 gtttctttaa ttttgaaata aaaaaatcga cgaaggtcga ttacgttttt gcacatgtcg
     1861 atttatccga cgtataagtg cgcccttacg ggatttaact agattataac gacgaatttt
     1921 agacctgtaa agcaataaca attaggtcta ccaggtagat aaagtgtaca aagtagaacc
     1981 gaaaaagtta tttattttga agatttttcg tagatataat ttgctaataa acttcctaaa
     2041 actgaataaa gaaaaatagt taaactcatg taatcactcc ataattctga agtgtctcca
     2101 tgtctacgca tagacacgca tttatgccga gaaaatttat tgtgcgttga gaagaaccct
     2161 taactaaact tgcagacgaa tgtcggcata gcgtgagcta ttaagccgac cattcgacaa
     2221 gttttgggat tgttaagggt tccgaggctc aacgtcaata aagcaattgg aataaagcac
     2281 ctatgattat ttaaaaatat aagttataat acattcaaaa atatttttga atgaggtgta
     2341 ttatgattca aacggttgtt gctgctgctg ttctttatat tgctacagca gttgatttat
     2401 tagtaatttt attaatattt tttgctagag caaagactag aaaagaatat agaaatattt
     2461 atgttggtca atatttaggg tctattattt taatattagt tagtttgttt ttagcttttg
     2521 tattaaatta tgttccagaa aagtggatat taggtttatt gggtttaata ccaatttatc
     2581 ttggaattaa agtggctatt tatgatgatt gtgaaggaga aaagagagct aaaaaagaat
     2641 tgaatgaaaa aggattgtct aaattagttg gtacggttgc aattgttacg atagcaagtt
     2701 gtggcgccga taatattggt ttatttgttc cttattttgt gacattaagt gttactaatt
     2761 tattacttac tctgtttgtc tttttaattt taattttctt cttggtgttt actgcacaaa
     2821 aattagctaa tatcccagga attggagaaa ttgttgagaa atttagtcgt tggattatgg
     2881 ctatcattta tatagcttta ggtttattta ttattattga aaatgacact attcaaacaa
     2941 ttttaggatt tatattttaa tttagggtgt gattttatat gagttatgaa aatacttgtg
     3001 atgtgatctg tgtacatgag gataaagtta acaatgcttt aagtttttta gaagatgata
     3061 aatctaagaa attacttaac attttagaaa aaatttgtga tgagaagaaa ttgaaaatca
     3121 tattatcttt gattaaagaa gatgagttgt gtgtttgtga tatttctttg atattgaaaa
     3181 tgagtgttgc ttcaacttca catcatttaa ggcttttata taaaaatgag gtacttgatt
     3241 tttataaaga tggaaagatg gcatattatt ttattaaaga cgatgaaata agagagtttt
     3301 tctctaaaaa tcaggagggt ttttgaaacg agtgaaacga gtttcttctt gtcttgatac
     3361 tataaaaaac aaagttatat aacataagac gacttatcgg aagttatcta tcatcgtata
     3421 ttcggagatg atataatata caggtcgcct atctctcagg cgtcaattca gacgcagaga
     3481 ggaggtgcat tactttgcta atcattttcg ttcacatcat agcaccagtc atcagtggct
     3541 gtgctgttgc gtattatact tattggctta gtaaacgcaa caaataatta ataggcgact
     3601 ttataagcca gcatcgaaaa aatccccaca ctattgcagt agtgtgggga ttggtgcatt
     3661 aattgttaat cattttcgtt atctagatta taacactggt tagtagagga atgcaaaaag
     3721 ataatatttt aagtagtctt tttggatatt atcatatgat atatttatgt aaattgattt
     3781 tttattaaag gagcatatat gagtaatact aaaattaaat atcaaatttt tatttcatca
     3841 acttatttag atttaataga agagcgtcaa gcagtagtag aagcagtttt aaacagtaat
     3901 aatattccag caggtatgga actttttaac tcaagtagta aaagccaatg ggaaataata
     3961 acacaatgga tcaatgaatc agatgcagtg atattattgt taggttctag ttatggaaca
     4021 attgatagtg aagaaaatat tagctataca gaaatggagt acaattttgc taaatcttta
     4081 ggtaaaccta tatatgtttt tgagatgaat aaagcatcta ccgaatccgc ggaaaagtct
     4141 aaaaaattag atgagtttaa gacaaaagtt aaagaaaata gactaattag tccaaatatt
     4201 aatgatattt cggaattaaa aagtaaagta tctcagactt taatgcaaag tgtacctagt
     4261 gaaattaaag aaaatagtgg atggataaga gtacaggatt gtggtaaaaa tgtaaaaaaa
     4321 ttaaaaaaat tggatgctat acaaagagat ttagatacat atgatttttt gaaagaagaa
     4381 ctttataaaa tattcatgta tgtaaaaact ttttatttcc agcacacacc atttgatgat
     4441 agttatattg aagatataga aaactacctg ctaaattatg aaaataatcc acataaatca
     4501 ttcatgaaaa taaaagttta tgattataaa aactttttaa aacatttaaa aaacatattg
     4561 attttaatta aacaaggaaa aaagccatta gctcaagccg gtcattttaa attaagcttt
     4621 tacgaagagg atttaaaatt gttacaaaaa aattataagg ctacgctagt cgatattaaa
     4681 ggattgaata aacattttta taaggaatgt gataggtgct taaaagcata taatattttt
     4741 attgagggta taaataaaca tttatatgaa tgatataagt acaccctaat catacatata
     4801 taattgttgt cactttactc gatatatgag aatgacttta cctaataaat gagaaaagca
     4861 acgcactata ttaaaatgcg ttgctttttc tattatcgaa tacctataac taaactatct
     4921 ctagctgttt ctacaacttc tgtacttatt ttatctaaac catttatatc cataattctg
     4981 tctatctgcg caaataaacg atgaatcagc ctaaaattcc cttttgtaat tttaataatc
     5041 gtcgttattg cctcataatc agtaaagtct tcgagtttta aatcaaatcc taagtcttgc
     5101 cacttatatt ctaatatatg atgcgtctca tctttactta gattatcaaa ttcatgcgca
     5161 aaacctatac gcgaatatag ttgaggataa cgagatagtt ttttctctat acccggcata
     5221 ccaataaata tcattgctaa attatgttca tcataaatag ctcttaattg ctctaaatgt
     5281 tgcactttaa ggcgatcaat ctcatctact ataattaaat caatatattt aaacatatca
     5341 ctataatgtt tactatgttc atgtccatac ttatttacaa catgtaattt ttgacccaaa
     5401 tcaatgattg cagtaatact atataaatca ttccttaatt tagtttgttt ttctgctggt
     5461 gcattataaa atattttatc caccgatagt atgtcatcgg tcgttttact agctaaatct
     5521 tcccaacctc tatggttaac ttgtttttca atagtatccc aatttgtgta atacctagac
     5581 gataaagttt ttccgacacc aggttgacca taacatatac caatatactg atatttaata
     5641 caagcatcac aaaattcagc aaagcgttta tactctttcg tttcaatgaa attttgattt
     5701 ttagtcatta cgatatctct ttaatttttt gacgttttta gtcgtttctt gaggtgaata
     5761 accacaattt ttttcttcct taattaattc cgtggtactt ggactagcaa tattttgttt
     5821 taagtgtttt cttctctgac tacgagcgtg ctgtatgtct tttatatcta tggagtaatc
     5881 agctatttct ggacatatag cagtacaaag aaattcatct ctataaaaca cacgtatttc
     5941 agccatatca tttgggttat aacgaatcag tacatattca cctacataag cttctagatt
     6001 agtattactg tatcgaaaac cttgaaaatg aatgccatct gaatgaactt ttctagattt
     6061 aggtatttcc aacaataata aatcaagttg ttctaagcta cttggcatat tagggaaaaa
     6121 gtgattacta ttccaatgac tgataggcgt ggtatgaata gtactatgtt ctttttgatt
     6181 ataatcttca attaaaaagt aacgtaattt ttcttcaaaa ttgtgaaaat ttattagatt
     6241 actaggagta tcattattgt ttatatatcc aggcagttgt tctaagaaag tttgattaac
     6301 tgtctgaaag aatcgttcta tttttccacg accacgagga acaccaactt tcgaaaacat
     6361 caagttaatt ttcaaatcaa tagcgacttg ttccatatga tgtgaagtaa agtcacttcc
     6421 atgatctgtg tagaattttt cgggtatacc acacactggc caattggtat catttttatt
     6481 ccatattgct tggtgtagtg ttaatgctgt gttttgagca ttggggggca tcaaaactta
     6541 agaaatagcc tgcaattgca cgactatagt catccataat aatagttagc catggtctat
     6601 tgatattacc tttttggtct aatataaaga tatctagcaa agtatggtct gcttgccata
     6661 tttcattggg tctcgaggat tctctaattt gtattaaatc gtacttattt tgatagtatt
     6721 tttcaccctg atgagagaaa tcaataacag acttaggcat cgctttaata acactataaa
     6781 tttgtttata acttggtata tcaaaattat tttctttaca gtaattagtg attttcctat
     6841 gaattgtggc tatactattt cttttattca taagcgcaag gcgttcaatt tcatcaatta
     6901 tctttggttc tattttacgt gttcctttat cagctcttgt cgcatagatt agtcctttaa
     6961 gtccttgttc tttatatgac ttattccacc tataaagggt gcttaacgca atccctttac
     7021 ttttagaaat acttgataaa gattgttcgc ctaggataaa aggttttata atattgtact
     7081 tttgagtagc ttttttacgt ttggcttcag aaaaattagt tagatacttc tccttgtttt
     7141 tcatcttcct tgtcctccaa tttattgagg taacgataaa ttgttgtacg tgaaacatgc
     7201 cactgttctg caattgtctt aattggcgtg ccattatcat aaagggtctt aagtaagttt
     7261 aaatcttttt gatttaattt ttctggtctg cctccatatc ggcctctagc acgtgctgca
     7321 attcggccag ctgaagaacg ctctaaaatt aaattacgtt caaattctgc aaatgctgcg
     7381 aataaatgaa ataataattg tccagttgaa cttgatttat ccattgttat attttcttct
     7441 aaactatgga aacttacacc tcgttcgtta agctcattaa ctaatgtgat taaatcctcc
     7501 atattacgtc ccaatctatc taatctccaa acaacaatcg tatctcctga cctcgcaaat
     7561 tcaattgctt tatctaaacc aggtctttta ctttttgaac cactcatatg gtcattaaat
     7621 atcttttcac aaccatatgt atttaatcga tcctcctgta aatttaaatt ttgtaatcca
     7681 gttgaaactc ttgcataacc tattttcaaa atacaaacct cctatataca tcttcagtgt
     7741 accataactt aattacaagg tcttttatga acatagattt tttgttgggt ttttgaacac
     7801 ataatcctat gtaattctta cttatttaga cataaaagaa gagtgtacag aaaatggtta
     7861 ttttctgtac actctcatct ttaataatat attttaaatg tcattaacaa aactaattta
     7921 ataagagtca agcatagttt acatatgtat tatgtttgaa acatacctct aattactttt
     7981 tactaatatc atttaaaata tctcgcaatt cttcaatttc tttgttattt aattcttcat
     8041 ttttcgcaaa attcaacact aaacttttca tatcccctcc atacagttta ttaagaaagg
     8101 ttttagcagt tttcatttta atatcgtctt ctttaatatt tgatgagtaa aaataaatat
     8161 tttctgattt gtatcgtttt ataatctctt ttttatatag tcttgtgatt aatgttctaa
     8221 tcgttttatc actaacttct ttatattttt gaatttcaac tacaatttca ttagctgata
     8281 ctgatttttt atcccatatt atattcataa catcccattc agccatagat atttcaactt
     8341 gcttattggc catttaaaac acccattcct tttaatattt tttcacttat taattcagca
     8401 tttttcccag acgggtttcc atctgataaa tgtgtagcaa aataatactt atcatgattt
     8461 gtaattacgt aacctacaaa ccacccatta ttatacttcc cgtttactat acctgtacct
     8521 gttttcccat acagttcata cttttcattt ttcttaatca ataatgaaga agataattga
     8581 ttctttgctt ttttactaaa atggttattt tgttccatca tatttttaaa aactattact
     8641 tgttcaagat tagatatttt caaactatct tccatccaat agcttttata acttcccaaa
     8701 ttttcattac cataatttaa ttgcttgagt tgagccgcag tatagttctt tggtatttga
     8761 ttactaatac gttcgaagta ccaattaact gaattttgca ttgctgtatt taaatcttgt
     8821 tccttattcc aagactcgaa aagataatgt ttatgattcc atgacatacg tgaattttta
     8881 tcacttataa tatgacggtc aagtccaaac agtgctaaat aaattttgta agttgaatca
     8941 ggagaatatc tttttctact ttctttttca ttataaatat agtatttgtc tttcttcatg
     9001 ctgtacataa cgaaagatcc actatttgaa ccaaaatttt tactttcatc taaaatttgg
     9061 taatcgcttt gtaagggctt tttataatta taatcagtta aggattgccc cattaaaaac
     9121 tggctttgaa ttatcatgat aaaaaaagta aaaatacaaa taaaaattag aattaatttt
     9181 gactgctttt tcagatcggc ttcttttata ttaattaatc tatttttgag taatgatttt
     9241 ttaccattaa atgaatggct taatatattt ttgttgaaaa atgaggtttt taatacggag
     9301 tccataatag attccgcata agttttaaat tcatttttgt taatattatt aaggacaaat
     9361 ctatcggctt ctacttcatt gtcatgaatt atctttcttt ttacaatatg tactaatgga
     9421 ttgtaactca taataataga gaagatatta aagataatta aatgtaaagt atctctattt
     9481 ttagcatgag catattcatg cagtattata tattttagtc ttttatcaat tacactttta
     9541 aaatatgaac taggaatcaa aataatatat ttcccatacc aaaaagttat tggagattga
     9601 atagcctctg cttttcgaat cacaatattt tttttatatt gatggttgaa aagtatcgta
     9661 tctattttat ttttttcatt ttcgtttaga taaagtgact gtttctttaa atatttaaga
     9721 tataataagg ctttcaaaaa tttaaaactt aaaataataa ctaaaactat ccaaataact
     9781 gtgcagatat tatcaattga atcccaatta aacttatgga tatctgttgc gaactcttga
     9841 acaggtttgg tggtaattat gttatggttc aagtcgtgtg acttactttc aactgtgggc
     9901 gcttgattat tcacattatt aaattttata aaagagaatt taattgggat aaaaggaatt
     9961 aatccagcaa gcacagttag ataccaaact ttataattta aactataatt aaaatagcgt
    10021 tttaatatgt acctaaaaaa tactaacaat agaaatataa aacaaaagct tactacactc
    10081 attattagta atttagccat tttgacacct tctttcaaat atttataata aacaattgac
    10141 atcaatatta caattgtaat attattgttt tgtaaaaatt acaactgtaa tatcggaggg
    10201 tttattttga aaaagttaat atttttaatt gtaatggctt tagttttaag tgcatgtaat
    10261 tcaaacagtt cacatgccaa agagttaaat gatttagaaa aaaaatataa tgctcatatt
    10321 ggtgtttatg ctttagatac taaaagtggt aaggaagtaa aatttaattc agataagaga
    10381 tttgcctatg cttcgacttc aaaagcgata aatagtgcta ttttgttaga acaagtacct
    10441 tataataagt taaataaaaa agtacatatt aacaaagatg atatagttgc ttattctcct
    10501 attttagaaa aatatgtagg aaaagacatc actttaaaag aacttattga ggcttcaatg
    10561 acatatagtg ataatacagc aaacaataaa attataaaag aaatcggtgg aatcaaaaaa
    10621 gttaaacaac gtctaaaaga actaggagat aaagtaacaa atccagttag atatgagata
    10681 gaattaaatt actattcgcc aaagagcaaa aaagatactt caacgcctgc tgctttcggc
    10741 aagactttaa ataaacttat cgcaaatgga aaattaagca aaaaaaataa aaatttctta
    10801 cttgatttaa tgtttaataa taaaaacgga gacactttaa ttaaagatgg tgttccaaaa
    10861 gactataagg ttgctgataa aagtggtcaa gcaataacat atgcttctag aaatgatgtt
    10921 gcttttgttt atcctaaggg ccaatctgaa cctattgttt tagtcatttt tacgaataaa
    10981 gacaataaaa gtgataagcc aaatgataag ttgataagtg aaaccgccaa gagtgtaatg
    11041 aaggaatttt aatattctaa atgcataata aatactgata acatcttata gtttgtatta
    11101 tattttctat tatcgttgac atgtataatt ttgatatcaa aacctgattt tccctttatt
    11161 attttcgaga tttattttct taattctctt taacaaacta gaaatattgt atatacaaaa
    11221 aatcataaat aatagatgaa tagtttaatt ataggtgttc atcaatcgaa aaagcaacgt
    11281 atcttattta aagtgcgttg cttttttctc atttataagg ttaaataatt ctcatatatc
    11341 aagcaaagtg acataattgt tgaatttata gtaactgaaa cgtatgatta gggtgtatat
    11401 taattatgag atggaaatca atgattgttg gctatgcgag agtatcatcg aacgatcaaa
    11461 atttagaaag acaacttgaa aatttaaaga cgttttgtgc tgaaaaaata tttacagaaa
    11521 aacagtctgg gaaatctatt gaaaatagac cagtacttca agaagcactt aactttttga
    11581 ggatgggaga tcggtttgta gtagaatcca ttgattgttt aggtcgtaat tatgatgaag
    11641 tggttggcaa tccattactg gataagttta tgaaagattt aattattcaa atattagcaa
    11701 tggtttcaga acaagagaga aatgaaagta aacgtcgaca agcgcaagga attcaagttg
    11761 cgaaggaaaa aggcgtatat aaaggggccc cttactttat tcaccaaatg ctaaagatcc
    11821 acagaaacgt attatttatc atcgcgttgt agaaatgtta gaagaaggcc aatcaattag
    11881 taaaattgcg aaagaagtaa atataacaag gcagacgatt tacagaatta aaaacgataa
    11941 tgatttgatt taataaactt tgtttcaatg caactgagag cttctcacag cgtctcaaaa
    12001 cagatgatta taatctatta tgagttttta ataataagac aagaaaagag agcgtaaata
    12061 tgctctctct ttttctcatt aacgaacgtt tataacgcaa tcatgatgag agtattaata
    12121 cagttaatta tttgaaatac tttagtttat ataatgttga attataggta gtgaaattgt
    12181 atttattata cattgtcata tcgttgttta atgatagtta atttaaggat aatagaatta
    12241 atattatata tagatgaaca aaacaagcaa cagagcagaa aaaacacttt tagattattt
    12301 ttactcattc tctattttat ttatttttgc tataataaat aaagaatatt aaatggaggt
    12361 gtttttatga aaaagaaatt tgtttctagt tgtattgcta gtacaatctt attcggcact
    12421 ttattaggtg taacttataa agctgaagct gcaacagtac atgtagctgg tggtgtttgg
    12481 agccatggta ttggaaaaca ttacgtatgg tcttattata gtcacaataa aagaaatcat
    12541 ggttcaacag cagtagggaa atattcatct tttagtggtg ttgctcgacc tggtgttcaa
    12601 tctaaggcat ctgctccaaa ggcttggggt ggaaacaaaa cattttatag tcttcactaa
    12661 ttacatatat taattaagga gaatagacag aattaagtaa acttaattct gtctattctc
    12721 ttgaaagagg caatatgaaa aaattcaaat tatttttaga ttttagtact ttattattaa
    12781 tttcagggct attattttta tttttcttca aagaaaatga agaaattata cctgaaagtt
    12841 ccaatatttt aacaatctct aattgggata aatctaacag taaatcaaaa gtgttagatg
    12901 tcattgaatc aggcgccaaa aatcaaaata tacaaataat taaatccgtt aaagattttg
    12961 ataataaaaa agaatttttt gttttcaact caaaacgaaa taattctgac tttatacgta
    13021 ataaaacatc acttttaact ccatcagatt tattaaacag agagataaaa ggaaagtatt
    13081 atattattgg tgaacatttc aatgtagaag agcttacgag tgaattaaaa aaagctggtt
    13141 taacatctga agttgaatac ataaatagaa acatattatt ttttgaatta gtcatagata
    13201 atgatttatt agtgcctttc atattcttag gcgtattata tcttttgtac ttcttatatg
    13261 acagaggata taatttgaaa ttttatgcta ttcaaaagct acatggcttt tcaactacac
    13321 gcattatttt tcataatatt catataaaaa tcatttattg gctagtactt accgctattt
    13381 tctttattgc taatatatta atcattcata tagccaattt agaattggat ttcttaatgt
    13441 ttattcgtag atttatcgtg ttgctttttg tttttgcttg tattactaca ttgctgtggc
    13501 tgatttcgta tagtcttctt ttgaagttaa gtattccatt aatcttaaaa gggaaaaaag
    13561 gatataagtt ttcaatattt atgggtacct tcaccaaatg tatcatggtg ctaatactat
    13621 cttttttact ggcacaaaat ttaaatgctt atacgaagtt aaagaatata tatgactctg
    13681 aaaaaatctg gcaaaagtta gataattact atacattaga gatttctcct aatattagaa
    13741 attcagaaga aaaacaaatt ttagaaacta atatatacaa tttaattaag aaaagcgaac
    13801 aacttggtgg attgctttta aagaataata atatagccaa tcccgataaa aacaattaca
    13861 ttcctgaaaa tggcaatgtc ttttttatca ataacaattt tctgaatttc tataaaaata
    13921 taaatcatga ttttaaaatg attgacaatc attcggataa aataaacgtg tttattcctc
    13981 caaggttaca atatcaaaaa tctgaaattc aaaaaaatca ccaaggatgg attgactttc
    14041 aaaagcaaca aaacaaaaaa gtagatgtgc aagtactgtc aaaaaacgta tctgtttttt
    14101 cttttgatcg cacaactaat ttgaaatttg ggtatctaga ttctccaata gtaatgttgt
    14161 tgacacttga cgatttaaca ggagattttt atttagctgc tgtttctcaa ggtggatatc
    14221 tatttaaaga tttagataca cttaaacagt tgcttaaaga taatcaccta gaagaagaaa
    14281 ttagcggtat aactaattac aaggattctg tgttaaatga attaaatgaa acgaaaacaa
    14341 aaatgataat aacaatagtt actattatta ttaacgtttt cattcttttg atagctactg
    14401 tatttgaaac aattcaatat tttagctgga ataaaaagca actattatta agaaaaattc
    14461 atggttattc attattcagc agtaatgtac gttatttaac aatttctatc ttactttcta
    14521 ttatgttagc ttacactacg catattttat ttggttcaaa gatattacta ttcataataa
    14581 tgtctattgc tattattcaa catttacttc aaattgctta tattaaatat cttgaaaaat
    14641 actttaaaga tttaatgagg gagatttaaa caatgaaaag aactttaatt attgtaacaa
    14701 ttgtactatt aatcatcata cttagtctgt tattggttag aaatgagcat ttagatcgtt
    14761 ttaacccact gttaaaagaa aaagtatctt atgccaaagt tccaaaagat acccaggact
    14821 atagaaatat aactgtatac tctaaaaatg gagaaaaaaa gagttataaa ttggattttt
    14881 taggttatga tcctacaaaa gaatatgtga aagtaaatca taaagggaaa tatgtgcgtt
    14941 cgatagaata cattacaaaa gatattccta gctttttaaa atgaggtgac cgatagtgat
    15001 aaaacttgaa aatgtttttg taaaaaaagg taataaaaac attttagacg gatgtaactt
    15061 taattttgaa aaaggaaaat catatgcttt agttggagaa agtggcgctg gaaaatctac
    15121 tttacttaac ataatcgctg ggttcgaaga tgtatcacaa ggaagcatat atattgaaga
    15181 taagttacta aaaaagaaag tcgattttta tagatatacg ttagggtatt tgtttcaaaa
    15241 ttttgcttta cttgaaaatc aaacaatcag tcaaaattta gatttagccc taaagttcaa
    15301 aaagaataaa aaagataata tgaacttaaa gaaagaagta ttaaaaaagg tcggcctaga
    15361 tttagatatc aagagaattg ttagttctct aagcggtggt gaacaacaaa gagtagcatt
    15421 ggcaagatta atattaaaag atccgaaaat cattttagct gatgaaccta ctgggtcact
    15481 agatacgaaa aatggaaaaa tagttattga tttattattg aagctacttg atgaaaataa
    15541 aactatgatt gtcgtaacgc atgatttaga gcttgcgaaa cgatttgatg tcatagtcaa
    15601 tatttctgag cttagaagtt aagaaatatt gataggtata gccatacata gagaaaaaac
    15661 aatatgaaat atgattagtc attacttttg agttttttaa tttaaggact ggattttcca
    15721 gtcctttttt atgatgacaa aaaacaagcc tcgcagagca cacgttttta atgacttatt
    15781 tggtttttaa taagtctagt atgttaaaac ttttctgtgt ttaggtgtta taatcatatg
    15841 cagtggaagt tgatttatta tgtcttattc aattattcga gttgttaaag tgaaatcaaa
    15901 aacaaataca agaggtattc aaagacatat tcaaagagaa aataaaaatt atgaaaatat
    15961 agatattgat ttaagtaagt catatttaaa ctatgattta gtcaatgata ctaagtttga
    16021 ttttaataaa aaaattgatg agaaaatcga aaaaaattat aaagggaaac gcaaaattag
    16081 aacagatgcc attaaacata ttgatggatt aattacatca gataatgtgt tttttaatca
    16141 attatctgaa gaagagacaa aatagttttt taagcatgct aaagcgttct tggaagaaga
    16201 atacggtaaa gaaaatcttt tatatgcgac agtacatatg gatgagataa caccacatat
    16261 gcattatggc gtagttccta ttactaaaga tggacgttta agtgcgaaag aagttgtagg
    16321 taataaaaaa gcattaaccg aattccaaga tagattcaat acgtatatta ataagcaagg
    16381 ctatgattta aaacgaggaa tttctagaca acttacaaaa gaaaaacatg atcaagttag
    16441 ccgttataaa caaaaaacag aatatcataa acaaatgcat atgcgctaga agcaaataga
    16501 ggattattta aagcaagaaa atgataaatt gatacaaaac taccaaaaat cattagatac
    16561 gcttaaaaag cttctgagtg tcatgtatga aattaaaact aaaaatgtag gtggatggtt
    16621 tcataaagag aagcaagaaa caggcaatat tgtaattact aaaactgatt ttgaaaaata
    16681 cactaaacaa attaaagcag cacagatgat attagatgat tatgaatgta ttaaaagtgg
    16741 gaagtcatta aaaaaagcag agaaacaaaa tgaaagttta gttaatgaat tgactagtgt
    16801 ccatatggaa aatgaaaagc tggttgaaga atttaatgat ttagcacaac gttataatta
    16861 tttgttgtca gagaatgaaa agaaagataa agaactaaat tatacgctta agttattcaa
    16921 tcaagttttt aaaatcatta aaagtatgat gaaagaagag cgttatcata cactaataaa
    16981 tcatattgat aatcatttag acaattctaa aatacgtgaa gtaatgacga tagataacaa
    17041 tgatgaacag ttctttaaga agaaatatca agcacaagaa cgagaaatca tttttaaaga
    17101 agatagagaa gatggttata cattataaaa atagatattt aaatgcattt gtttgaggtt
    17161 aattattttt taggatgaaa aataggtagc ccccctcgaa ctttttaata tatttttata
    17221 aatcttttta aaaacctttt taggggcttg ttcagcccta gagcaacaag ggatttcaat
    17281 gcctaaagga gtgtttttgc cacataaaag gagtgttttt gccacataaa aggagtgttt
    17341 ttgccacata aaaggagtgt ttttgccaca taaaaggagt gttttaaaaa gttgtactcc
    17401 ttttatggtt tgacattgat aataaagtag tttattgtga ataaaagttt tcaaatggga
    17461 gtgtatattc aaacgatact cctttaggtt cgtaataaag ggggctgtat catgacagga
    17521 gagacagttg tatataaaaa tgaaatgaat ttagttccat taaggcgatt tacagctact
    17581 gaaattaatt tattttttgc gatgtgtaat aaattaaaag agcaagatac taatacatta
    17641 cgtttgtctt ttgatgaatt aaaaaaatta agtaattata gtccagaaac acgtaatatt
    17701 aatagatttg ctaatgattt agataatgtg tataaaaaga tgttgaattt aaccattaga
    17761 tatgaagatg atgatgtaat agaaagattc gttttattca atcattatag aatccataag
    17821 cgagaacaat atctagaaat atcaacttct tcaaatttaa aacacatatt gaattcaatt
    17881 actaataatt ttacaaaatt tgaattaaaa gaaatgacta ggcttaaatc tacatattct
    17941 aaaaatatgt ttagattact taaacaatac aaacatacag gctatttaaa aattcacatc
    18001 gatgatttta aaaatcgttt agatatacct aaatcttatc gtatgactga tattaacaaa
    18061 aatgttttta aacctattat tattgaatta ggttctatat ttaataatct tactatcaat
    18121 aaaatcaaag cgaaaaaagg gcgtaaaatt gaatggatag agttcacatt tgacgctgag
    18181 aaacgcattc ataacaaacg acaacctcaa atgtccaaga tagataaatc aagacaatat
    18241 gtgaggcgtg aaaagacgcc taaatggtta gaagaacggt catatgaaaa gcaacctcaa
    18301 aaagattacg acccacaatt agagaaagaa cgagaggatt ttttaaaaca acttgaactg
    18361 aattgggaat aacgcaactt ttcgttgcga gttggttaac taaaagtatc gtgacttttc
    18421 aattatttta attatagttt gagttaagga gtgattttat gaaattagct gaagctataa
    18481 aagaacagag agaattaaaa ggatggtctc aagaggaatt agccaatata ttaaaggttt
    18541 ccagacaaag tgtttctaaa tgggaaagtg ccaaaaacta tccttcctta gatattttga
    18601 ttgctatgag cgatttattc ggaatctctt tagagcattt aattaaagga gatgcacgtt
    18661 ttaaaaaagt tattttagag ggaaattata gagagagtaa cagggtacct tccatagttg
    18721 attttcttta tgatttttgg tggcttttct ttcctgttgc cggattttta gtttgggcca
    18781 ttcattcgtt catgggttga ttttaaaatc aaaatttaat gcacatcagg caatatgaaa
    18841 aaggaaagta tttttacgac accttgaaat gtattgataa gaatagaaga gtttacaaaa
    18901 ggcaatatga aatatatact aaaagtaaca agaatttcgt ttgtaaacgc accaaacccc
    18961 gaactattcg ccgtagttcg gggttttttg gtgaattggc tttaatcgtc tatattattt
    19021 tttatcacgt ttatggagct aataaccaaa taacgtaacg agacaaccac tgattgccgt
    19081 tgtcatgatg tgaacaagaa tttcattcat aaatcgcacc tcctctctac gccgaattga
    19141 catctgagag atagacgacc ttcacattat aacaaatctc tccttccatg aaataacatg
    19201 aatattaata aatgttgtta tatattaaag ataaataaat ataagggtgg gattgataat
    19261 gtttggcgat caaagacaag aagctacaaa atatgtcatt aaagaaggtt atcaagatat
    19321 ttatttcctc aacaaaaacg gggaatggta ttatttcgaa gtaagaagtg tctggcgtgg
    19381 caaacatatt attagagtta aagatggttt gttaggttgg agaaaagaaa tagtaactga
    19441 ataaagatac aaaatacttg ccaatgcaaa agaatgaaaa cgcgtcaaat caacgatgta
    19501 taaaagatgc ataaaagata atgaaagtaa aaagccataa atgccgtcat ataggcattt
    19561 atggcttttt gtttggttaa tataatcgat gattatcaga agttcaattc acataaaaaa
    19621 gcacggctaa tttatgaact tgtcgtacct ttttaattaa aattagttac caaatagacg
    19681 actccaaaac ccctttttag cttccgtttc ttcactcttt tcttctatat cactatcatt
    19741 gtcatttgga ttagctgaaa cgtcttctct ttgtgcttct tcaccttgtg tactttcgtc
    19801 gctttgactt ccagatatat ccttatattg aatctctgtt tctttagttt cttcctgatc
    19861 tcggttaata tttacaggac tagtagtata tgttttttct tgtgcatcaa cgttttgtct
    19921 agcgttagta gatgtatcaa agggataatt aaggtgtttt tctttttcta attgattttc
    19981 taatttttga atctttttat tactctctaa tgccagaact tgctgattct caagcaattt
    20041 tgatctttct tgcaactctt ttctgtaaaa ttcaattgtt gtttcatgac gactttcttg
    20101 tttttcaatt tgattattta aagtattaat ctgattttta agtatttcta ctatttgagc
    20161 atcgctttta gattcaaaat taatattatc cgactctttt ttttgtttgt cttcagtagc
    20221 actttcaaaa ccatatttct ttttattttc atttactctt tgaatgattt tttctatatc
    20281 ggtatcttct ttaataaacg aagtgttttc ctgttttatt gtctctatat tcaatctctt
    20341 aatattatta aaaatcgttt gcttactaac ccctaattct tcactcaacc tttttacact
    20401 tttcatatca tcaacccttt ataaaatgtt tataaaccct tagtaaaata ttataagctt
    20461 ataacgatta ataataaagt aatcgacaaa taaaaatata aattcgccaa cctttttatc
    20521 aagggtttgt aaagggttta tggtgtttat caaaggttta taaacctata ttttcccttt
    20581 tttaagttta ttcaaagtgc tataataacc ttaatataac aaaaacccca actactggta
    20641 atagtcgggg t
//
