LOCUS       NZ_CP047838            20654 bp    DNA     circular CON 18-DEC-2023
DEFINITION  Staphylococcus aureus strain UP_719 plasmid unnamed, complete
            sequence.
ACCESSION   NZ_CP047838
VERSION     NZ_CP047838.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN11175614
            Assembly: GCF_009912695.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20654)
  AUTHORS   Dilthey,A., Meyer,S. and Kaasch,A.
  TITLE     Increasing the efficiency of long-read sequencing for hybrid
            assembly with k-mer-based multiplexing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20654)
  AUTHORS   Dilthey,A., Meyer,S. and Kaasch,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-JAN-2020) Institute for Medical Microbiology and
            Hospital Hygene, University Hospital Duesseldorf, Moorenstrasse 5,
            Duesseldorf 40225, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler Hybrid Assembly v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 43x / 446x
            Sequencing Technology :: Illumina MiSeq / Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/17/2023 04:22:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,779
            CDSs (total)                      :: 2,698
            Genes (coding)                    :: 2,621
            CDSs (with protein)               :: 2,621
            Genes (RNA)                       :: 81
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 50 of 77
            Pseudo Genes (incomplete)         :: 26 of 77
            Pseudo Genes (internal stop)      :: 13 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047838.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20654
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="UP_719"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /plasmid="unnamed"
     gene            166..441
                     /locus_tag="E3S86_RS13990"
                     /old_locus_tag="E3S86_13715"
     CDS             166..441
                     /locus_tag="E3S86_RS13990"
                     /old_locus_tag="E3S86_13715"
                     /inference="COORDINATES: protein motif:HMM:NF018653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="WP_160175356.1"
                     /translation="MSIKLYNINEEYREKFYQIPKVFFTNPKYIKLSNDAKMAWGILRD
                     RLDLSIKNGWIDSKTGNIYFYYKNENPQNILNCKKDKVIKIKKELN"
                     /name="replication initiator protein A"
     gene            <853..1135
                     /locus_tag="E3S86_RS13995"
                     /old_locus_tag="E3S86_13725"
                     /pseudo=""
     CDS             <853..1135
                     /locus_tag="E3S86_RS13995"
                     /old_locus_tag="E3S86_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            2344..2961
                     /gene="cadD"
                     /locus_tag="E3S86_RS13730"
                     /old_locus_tag="E3S86_13730"
     ARG             2345..2958
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            2980..3327
                     /locus_tag="E3S86_RS13735"
                     /old_locus_tag="E3S86_13735"
     CDS             2980..3327
                     /locus_tag="E3S86_RS13735"
                     /old_locus_tag="E3S86_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119691.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            3496..3588
                     /locus_tag="E3S86_RS13740"
                     /old_locus_tag="E3S86_13740"
     CDS             3496..3588
                     /locus_tag="E3S86_RS13740"
                     /old_locus_tag="E3S86_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3800..4774
                     /locus_tag="E3S86_RS13745"
                     /old_locus_tag="E3S86_13745"
     CDS             3800..4774
                     /locus_tag="E3S86_RS13745"
                     /old_locus_tag="E3S86_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WP_000072390.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSGWI
                     RVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN
                     YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL
                     LQKNYKATLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
                     /name="DUF4062 domain-containing protein"
     gene            complement(4894..5709)
                     /locus_tag="E3S86_RS13750"
                     /old_locus_tag="E3S86_13750"
     CDS             complement(4894..5709)
                     /locus_tag="E3S86_RS13750"
                     /old_locus_tag="E3S86_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000164958.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(5702..7145)
                     /locus_tag="E3S86_RS13755"
                     /old_locus_tag="E3S86_13755"
                     /pseudo=""
     CDS             complement(5702..7145)
                     /locus_tag="E3S86_RS13755"
                     /old_locus_tag="E3S86_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /name="DDE-type integrase/transposase/recombinase"
     gene            complement(7117..7710)
                     /locus_tag="E3S86_RS13760"
                     /old_locus_tag="E3S86_13760"
     CDS             complement(7117..7710)
                     /locus_tag="E3S86_RS13760"
                     /old_locus_tag="E3S86_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690638.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            complement(7974..8354)
                     /gene="blaI"
                     /locus_tag="E3S86_RS13765"
                     /old_locus_tag="E3S86_13765"
     ARG             complement(7978..8354)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(8344..10101)
                     /gene="blaR1"
                     /locus_tag="E3S86_RS13770"
                     /old_locus_tag="E3S86_13770"
     ARG             complement(8348..10101)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.56"
                     /coverage="100.0"
     gene            10208..11053
                     /gene="blaZ"
                     /locus_tag="E3S86_RS13775"
                     /old_locus_tag="E3S86_13775"
     ARG             10209..11050
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            11422..11955
                     /locus_tag="E3S86_RS13780"
                     /old_locus_tag="E3S86_13780"
     CDS             11422..11955
                     /locus_tag="E3S86_RS13780"
                     /old_locus_tag="E3S86_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823239.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001823239.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVVGNPLLDKFMKDLIIQILAMVSEQERNESK
                     RRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRIIYHRVVEMLEEGQSISKIAKEVNIT
                     RQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            12370..12663
                     /locus_tag="E3S86_RS13785"
                     /old_locus_tag="E3S86_13785"
     CDS             12370..12663
                     /locus_tag="E3S86_RS13785"
                     /old_locus_tag="E3S86_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000726502.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            12738..14672
                     /locus_tag="E3S86_RS13790"
                     /old_locus_tag="E3S86_13790"
     CDS             12738..14672
                     /locus_tag="E3S86_RS13790"
                     /old_locus_tag="E3S86_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000713729.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS
                     DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI
                     FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL
                     IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI
                     FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEK
                     QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD
                     FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD
                     RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS
                     GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH
                     GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK
                     YFKDLMREI"
                     /name="bacteriocin-associated integral membrane family
                     protein"
     gene            14676..14987
                     /locus_tag="E3S86_RS13795"
                     /old_locus_tag="E3S86_13795"
     CDS             14676..14987
                     /locus_tag="E3S86_RS13795"
                     /old_locus_tag="E3S86_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WP_000831610.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFLK"
                     /name="YxeA family protein"
     gene            14999..15625
                     /locus_tag="E3S86_RS13800"
                     /old_locus_tag="E3S86_13800"
     CDS             14999..15625
                     /locus_tag="E3S86_RS13800"
                     /old_locus_tag="E3S86_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000593103.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            15863..16492
                     /gene="mobV"
                     /locus_tag="E3S86_RS14000"
                     /pseudo=""
     CDS             15863..16492
                     /gene="mobV"
                     /locus_tag="E3S86_RS14000"
                     /inference="COORDINATES: protein motif:HMM:NF013258.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            16586..17131
                     /locus_tag="E3S86_RS13815"
                     /old_locus_tag="E3S86_13815"
     CDS             16586..17131
                     /locus_tag="E3S86_RS13815"
                     /old_locus_tag="E3S86_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000273005.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMILD
                     DYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKE
                     LNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQ
                     AQEREIIFKEDREDGYTL"
                     /name="hypothetical protein"
     gene            complement(17173..17457)
                     /locus_tag="E3S86_RS13820"
                     /old_locus_tag="E3S86_13820"
     CDS             complement(17173..17457)
                     /locus_tag="E3S86_RS13820"
                     /old_locus_tag="E3S86_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001793642.1"
                     /translation="MKTFIHNKLLYYQCQTIKGVQLFKTLLLCGKNTPFMWQKHSFYVA
                     KTLLLCGKNTPLGIEIPCCSRAEQAPKKVFKKIYKNILKSSRGATYFSS"
                     /name="hypothetical protein"
     gene            17515..18375
                     /locus_tag="E3S86_RS13825"
                     /old_locus_tag="E3S86_13825"
     CDS             17515..18375
                     /locus_tag="E3S86_RS13825"
                     /old_locus_tag="E3S86_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_000145511.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKEREDFLKQL
                     ELNWE"
                     /name="replication initiation protein"
     gene            18462..18803
                     /locus_tag="E3S86_RS13830"
                     /old_locus_tag="E3S86_13830"
     CDS             18462..18803
                     /locus_tag="E3S86_RS13830"
                     /old_locus_tag="E3S86_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000761341.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(19042..19113)
                     /locus_tag="E3S86_RS13835"
                     /old_locus_tag="E3S86_13835"
     CDS             complement(19042..19113)
                     /locus_tag="E3S86_RS13835"
                     /old_locus_tag="E3S86_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106873653.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            19262..19447
                     /locus_tag="E3S86_RS13840"
                     /old_locus_tag="E3S86_13840"
     CDS             19262..19447
                     /locus_tag="E3S86_RS13840"
                     /old_locus_tag="E3S86_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000466619.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(19666..20409)
                     /locus_tag="E3S86_RS13845"
                     /old_locus_tag="E3S86_13845"
     CDS             complement(19666..20409)
                     /locus_tag="E3S86_RS13845"
                     /old_locus_tag="E3S86_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843757.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR
                     QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND
                     NDSDIEEKSEETEAKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     ARG             10209..11053
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     BGC             7370..17663
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1193..1340
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="2.96e-25"
     Typing          17516..18375
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19667..20409)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          15864..16138
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 ttggtaataa aacgtttaga aactgtttat ttatataatt ttcgatactt agattatata
       61 atgaataata tcttagtgca agcacctaga ttattaaata aacacaaaaa atgtaagcgt
      121 tttattacct cttaccaaac taaaattcgg aaagaggttt ttattatgtc aattaaactt
      181 tacaacataa atgaggaata cagagaaaag ttttatcaaa taccgaaagt attttttaca
      241 aatcctaaat atatcaagtt atccaatgat gcaaaaatgg catggggtat tcttagagat
      301 cgtttagatt tatcaattaa aaatggttgg attgattcta aaacaggtaa tatttatttt
      361 tattacaaaa atgaaaaccc acaaaatata ttaaattgta aaaaagataa agtaattaaa
      421 ataaagaaag aacttaattg aagccgaatt actactacaa gaaagacaag gtttaaataa
      481 gacaaataaa ctttatttag ttaaaccaga agtaacagaa gatgatattt ataaaattca
      541 aaagcaagaa aacactgttg agagcagcaa tggcaaggaa gtcggaaaaa ccgacttcaa
      601 gaagtcgata aaacagaatc taatgatact gaacttagct atactgaatc taacaatact
      661 gaaataaatg atatgaatga cttgaatgat atagagtccg aaaatgaaat tagtaatcat
      721 tcaagtcaat gcaaggttat ttaatgcaaa caattttaac tgaatttgaa gaaacacatg
      781 cactttatat gcgccgtaaa aacatgaaac aatataatat ctttaatcaa tcattcaaat
      841 gattttataa caatcaaaga tacatcaaaa tatttcgtta aatcatctga ctttttaact
      901 gatagcgatg aaaaaatcaa gaaattgtca gtgatctaga aaaagatttg tatcgaaaac
      961 gtatgttaag ttatggtgga ttgcttaaat aaaaacataa aattttaaac ttagacgatg
     1021 ctgaagatgg caatttaatt aatacaagtg atgaagataa aatagcaaat gaagaagaaa
     1081 aggcacattc gattacggca attttgaaaa gcaaaattat tatttaaggc attagtgata
     1141 actgatgtct ttttttatta cttttttgta aaaaaagtgc tgtcttattt ttgtgacaaa
     1201 tgctgtatgt agtgtcacaa aagtgtgaca cttgagcttt ttatgacccc acacaaaaac
     1261 attttcgaag ccttgaaata tctggctttg ccagatcccc cattcattga tggggacaaa
     1321 tttcccttat gctcttacgg agattttaga gttaaattaa aatttctaag tttttgataa
     1381 gaaaagccct gcaggaattt agaagaacgt ggggaagttt taaagaattt tatgctaatt
     1441 tttaatttgc atgtaaatgg gcagtgtcta aaaaatagtt attgtttttg ctcaaatttt
     1501 tgtttttgaa aatagcatat atttatttgg ctcatatttg cgttttaaaa gcgtatataa
     1561 gttttagata aaactatatg aatcacccct aaatctttaa aatgccctct taaaattcaa
     1621 aataagggca taaaaaataa aagccccccc agataaagtg aggtagcttt ttctttcgac
     1681 gccgtatcgt cgatgtaatt caaaaaagtt atgggctata aatctacatc actttttcaa
     1741 gaatgtagta gcaatattca acgcaaatta atttttataa ctgctcggaa tatctcaagc
     1801 cgtttcttta attttgaaat aaaaaaatcg acgaaggtcg attacgtttt tgcacatgtc
     1861 gatttatccg acgtataagt gcgcccttac gggatttaac tagattataa cgacgaattt
     1921 tagacctgta aagcaataac aattaggtct accaggtaga taaagtgtac aaagtagaac
     1981 cgaaaaagtt atttattttg aagatttttc gtagatataa tttgctaata aacttcctaa
     2041 aactgaataa agaaaaatag ttaaactcat gtaatcactc cataattctg aagtgtctcc
     2101 atgtctacgc atagacacgc atttatgccg agaaaattta ttgtgcgttg agaagaaccc
     2161 ttaactaaac ttgcagacga atgtcggcat agcgtgagct attaagccga ccattcgaca
     2221 agttttggga ttgttaaggg ttccgaggct caacgtcaat aaagcaattg gaataaagca
     2281 cctatgatta tttaaaaata taagttataa tacattcaaa aatatttttg aatgaggtgt
     2341 attatgattc aaacggttgt tgctgctgct gttctttata ttgctacagc agttgattta
     2401 ttagtaattt tattaatatt ttttgctaga gcaaagacta gaaaagaata tagaaatatt
     2461 tatgttggtc aatatttagg gtctattatt ttaatattag ttagtttgtt tttagctttt
     2521 gtattaaatt atgttccaga aaagtggata ttaggtttat tgggtttaat accaatttat
     2581 cttggaatta aagtggctat ttatgatgat tgtgaaggag aaaagagagc taaaaaagaa
     2641 ttgaatgaaa aaggattgtc taaattagtt ggtacggttg caattgttac gatagcaagt
     2701 tgtggcgccg ataatattgg tttatttgtt ccttattttg tgacattaag tgttactaat
     2761 ttattactta ctctgtttgt ctttttaatt ttaattttct tcttggtgtt tactgcacaa
     2821 aaattagcta atatcccagg aattggagaa attgttgaga aatttagtcg ttggattatg
     2881 gctatcattt atatagcttt aggtttattt attattattg aaaatgacac tattcaaaca
     2941 attttaggat ttatatttta atttagggtg tgattttata tgagttatga aaatacttgt
     3001 gatgtgatct gtgtacatga ggataaagtt aacaatgctt taagtttttt agaagatgat
     3061 aaatctaaga aattacttaa cattttagaa aaaatttgtg atgagaagaa attgaaaatc
     3121 atattatctt tgattaaaga agatgagttg tgtgtttgtg atatttcttt gatattgaaa
     3181 atgagtgttg cttcaacttc acatcattta aggcttttat ataaaaatga ggtacttgat
     3241 ttttataaag atggaaagat ggcatattat tttattaaag acgatgaaat aagagagttt
     3301 ttctctaaaa atcaggaggg tttttgaaac gagtgaaacg agtttcttct tgtcttgata
     3361 ctataaaaaa caaagttata taacataaga cgacttatcg gaagttatct atcatcgtat
     3421 attcggagat gatataatat acaggtcgcc tatctctcag gcgtcaattc agacgcagag
     3481 aggaggtgca ttactttgct aatcattttc gttcacatca tagcaccagt catcagtggc
     3541 tgtgctgttg cgtattatac ttattggctt agtaaacgca acaaataatt aataggcgac
     3601 tttataagcc agcatcgaaa aaatccccac actattgcag tagtgtgggg attggtgcat
     3661 taattgttaa tcattttcgt tatctagatt ataacactgg ttagtagagg aatgcaaaaa
     3721 gataatattt taagtagtct ttttggatat tatcatatga tatatttatg taaattgatt
     3781 ttttattaaa ggagcatata tgagtaatac taaaattaaa tatcaaattt ttatttcatc
     3841 aacttattta gatttaatag aagagcgtca agcagtagta gaagcagttt taaacagtaa
     3901 taatattcca gcaggtatgg aactttttaa ctcaagtagt aaaagccaat gggaaataat
     3961 aacacaatgg atcaatgaat cagatgcagt gatattattg ttaggttcta gttatggaac
     4021 aattgatagt gaagaaaata ttagctatac agaaatggag tacaattttg ctaaatcttt
     4081 aggtaaacct atatatgttt ttgagatgaa taaagcatct accgaatccg cggaaaagtc
     4141 taaaaaatta gatgagttta agacaaaagt taaagaaaat agactaatta gtccaaatat
     4201 taatgatatt tcggaattaa aaagtaaagt atctcagact ttaatgcaaa gtgtacctag
     4261 tgaaattaaa gaaaatagtg gatggataag agtacaggat tgtggtaaaa atgtaaaaaa
     4321 attaaaaaaa ttggatgcta tacaaagaga tttagataca tatgattttt tgaaagaaga
     4381 actttataaa atattcatgt atgtaaaaac tttttatttc cagcacacac catttgatga
     4441 tagttatatt gaagatatag aaaactacct gctaaattat gaaaataatc cacataaatc
     4501 attcatgaaa ataaaagttt atgattataa aaacttttta aaacatttaa aaaacatatt
     4561 gattttaatt aaacaaggaa aaaagccatt agctcaagcc ggtcatttta aattaagctt
     4621 ttacgaagag gatttaaaat tgttacaaaa aaattataag gctacgctag tcgatattaa
     4681 aggattgaat aaacattttt ataaggaatg tgataggtgc ttaaaagcat ataatatttt
     4741 tattgagggt ataaataaac atttatatga atgatataag tacaccctaa tcatacatat
     4801 ataattgttg tcactttact cgatatatga gaatgacttt acctaataaa tgagaaaagc
     4861 aacgcactat attaaaatgc gttgcttttt ctattatcga atacctataa ctaaactatc
     4921 tctagctgtt tctacaactt ctgtacttat tttatctaaa ccatttatat ccataattct
     4981 gtctatctgc gcaaataaac gatgaatcag cctaaaattc ccttttgtaa ttttaataat
     5041 cgtcgttatt gcctcataat cagtaaagtc ttcgagtttt aaatcaaatc ctaagtcttg
     5101 ccacttatat tctaatatat gatgcgtctc atctttactt agattatcaa attcatgcgc
     5161 aaaacctata cgcgaatata gttgaggata acgagatagt tttttctcta tacccggcat
     5221 accaataaat atcattgcta aattatgttc atcataaata gctcttaatt gctctaaatg
     5281 ttgcacttta aggcgatcaa tctcatctac tataattaaa tcaatatatt taaacatatc
     5341 actataatgt ttactatgtt catgtccata cttatttaca acatgtaatt tttgacccaa
     5401 atcaatgatt gcagtaatac tatataaatc attccttaat ttagtttgtt tttctgctgg
     5461 tgcattataa aatattttat ccaccgatag tatgtcatcg gtcgttttac tagctaaatc
     5521 ttcccaacct ctatggttaa cttgtttttc aatagtatcc caatttgtgt aatacctaga
     5581 cgataaagtt tttccgacac caggttgacc ataacatata ccaatatact gatatttaat
     5641 acaagcatca caaaattcag caaagcgttt atactctttc gtttcaatga aattttgatt
     5701 tttagtcatt acgatatctc tttaattttt tgacgttttt agtcgtttct tgaggtgaat
     5761 aaccacaatt tttttcttcc ttaattaatt ccgtggtact tggactagca atattttgtt
     5821 ttaagtgttt tcttctctga ctacgagcgt gctgtatgtc ttttatatct atggagtaat
     5881 cagctatttc tggacatata gcagtacaaa gaaattcatc tctataaaac acacgtattt
     5941 cagccatatc atttgggtta taacgaatca gtacatattc acctacataa gcttctagat
     6001 tagtattact gtatcgaaaa ccttgaaaat gaatgccatc tgaatgaact tttctagatt
     6061 taggtatttc caacaataat aaatcaagtt gttctaagct acttggcata ttagggaaaa
     6121 agtgattact attccaatga ctgataggcg tggtatgaat agtactatgt tctttttgat
     6181 tataatcttc aattaaaaag taacgtaatt tttcttcaaa attgtgaaaa tttattagat
     6241 tactaggagt atcattattg tttatatatc caggcagttg ttctaagaaa gtttgattaa
     6301 ctgtctgaaa gaatcgttct atttttccac gaccacgagg aacaccaact ttcgaaaaca
     6361 tcaagttaat tttcaaatca atagcgactt gttccatatg atgtgaagta aagtcacttc
     6421 catgatctgt gtagaatttt tcgggtatac cacacactgg ccaattggta tcatttttat
     6481 tccatattgc ttggtgtagt gttaatgctg tgttttgagc attggggggc atcaaaactt
     6541 aagaaatagc ctgcaattgc acgactatag tcatccataa taatagttag ccatggtcta
     6601 ttgatattac ctttttggtc taatataaag atatctagca aagtatggtc tgcttgccat
     6661 atttcattgg gtctcgagga ttctctaatt tgtattaaat cgtacttatt ttgatagtat
     6721 ttttcaccct gatgagagaa atcaataaca gacttaggca tcgctttaat aacactataa
     6781 atttgtttat aacttggtat atcaaaatta ttttctttac agtaattagt gattttccta
     6841 tgaattgtgg ctatactatt tcttttattc ataagcgcaa ggcgttcaat ttcatcaatt
     6901 atctttggtt ctattttacg tgttccttta tcagctcttg tcgcatagat tagtccttta
     6961 agtccttgtt ctttatatga cttattccac ctataaaggg tgcttaacgc aatcccttta
     7021 cttttagaaa tacttgataa agattgttcg cctaggataa aaggttttat aatattgtac
     7081 ttttgagtag cttttttacg tttggcttca gaaaaattag ttagatactt ctccttgttt
     7141 ttcatcttcc ttgtcctcca atttattgag gtaacgataa attgttgtac gtgaaacatg
     7201 ccactgttct gcaattgtct taattggcgt gccattatca taaagggtct taagtaagtt
     7261 taaatctttt tgatttaatt tttctggtct gcctccatat cggcctctag cacgtgctgc
     7321 aattcggcca gctgaagaac gctctaaaat taaattacgt tcaaattctg caaatgctgc
     7381 gaataaatga aataataatt gtccagttga acttgattta tccattgtta tattttcttc
     7441 taaactatgg aaacttacac ctcgttcgtt aagctcatta actaatgtga ttaaatcctc
     7501 catattacgt cccaatctat ctaatctcca aacaacaatc gtatctcctg acctcgcaaa
     7561 ttcaattgct ttatctaaac caggtctttt actttttgaa ccactcatat ggtcattaaa
     7621 tatcttttca caaccatatg tatttaatcg atcctcctgt aaatttaaat tttgtaatcc
     7681 agttgaaact cttgcataac ctattttcaa aatacaaacc tcctatatac atcttcagtg
     7741 taccataact taattacaag gtcttttatg aacatagatt ttttgttggg tttttgaaca
     7801 cataatccta tgtaattctt acttatttag acataaaaga agagtgtaca gaaaatggtt
     7861 attttctgta cactctcatc tttaataata tattttaaat gtcattaaca aaactaattt
     7921 aataagagtc aagcatagtt tacatatgta ttatgtttga aacatacctc taattacttt
     7981 ttactaatat catttaaaat atctcgcaat tcttcaattt ctttgttatt taattcttca
     8041 tttttcgcaa aattcaacac taaacttttc atatcccctc catacagttt attaagaaag
     8101 gttttagcag ttttcatttt aatatcgtct tctttaatat ttgatgagta aaaataaata
     8161 ttttctgatt tgtatcgttt tataatctct tttttatata gtcttgtgat taatgttcta
     8221 atcgttttat cactaacttc tttatatttt tgaatttcaa ctacaatttc attagctgat
     8281 actgattttt tatcccatat tatattcata acatcccatt cagccataga tatttcaact
     8341 tgcttattgg ccatttaaaa cacccattcc ttttaatatt ttttcactta ttaattcagc
     8401 atttttccca gacgggtttc catctgataa atgtgtagca aaataatact tatcatgatt
     8461 tgtaattacg taacctacaa accacccatt attatacttc ccgtttacta tacctgtacc
     8521 tgttttccca tacagttcat acttttcatt tttcttaatc aataatgaag aagataattg
     8581 attctttgct tttttactaa aatggttatt ttgttccatc atatttttaa aaactattac
     8641 ttgttcaaga ttagatattt tcaaactatc ttccatccaa tagcttttat aacttcccaa
     8701 attttcatta ccataattta attgcttgag ttgagccgca gtatagttct ttggtatttg
     8761 attactaata cgttcgaagt accaattaac tgaattttgc attgctgtat ttaaatcttg
     8821 ttccttattc caagactcga aaagataatg tttatgattc catgacatac gtgaattttt
     8881 atcacttata atatgacggt caagtccaaa cagtgctaaa taaattttgt aagttgaatc
     8941 aggagaatat ctttttctac tttctttttc attataaata tagtatttgt ctttcttcat
     9001 gctgtacata acgaaagatc cactatttga accaaaattt ttactttcat ctaaaatttg
     9061 gtaatcgctt tgtaagggct ttttataatt ataatcagtt aaggattgcc ccattaaaaa
     9121 ctggctttga attatcatga taaaaaaagt aaaaatacaa ataaaaatta gaattaattt
     9181 tgactgcttt ttcagatcgg cttcttttat attaattaat ctatttttga gtaatgattt
     9241 tttaccatta aatgaatggc ttaatatatt tttgttgaaa aatgaggttt ttaatacgga
     9301 gtccataata gattccgcat aagttttaaa ttcatttttg ttaatattat taaggacaaa
     9361 tctatcggct tctacttcat tgtcatgaat tatctttctt tttacaatat gtactaatgg
     9421 attgtaactc ataataatag agaagatatt aaagataatt aaatgtaaag tatctctatt
     9481 tttagcatga gcatattcat gcagtattat atattttagt cttttatcaa ttacactttt
     9541 aaaatatgaa ctaggaatca aaataatata tttcccatac caaaaagtta ttggagattg
     9601 aatagcctct gcttttcgaa tcacaatatt ttttttatat tgatggttga aaagtatcgt
     9661 atctatttta tttttttcat tttcgtttag ataaagtgac tgtttcttta aatatttaag
     9721 atataataag gctttcaaaa atttaaaact taaaataata actaaaacta tccaaataac
     9781 tgtgcagata ttatcaattg aatcccaatt aaacttatgg atatctgttg cgaactcttg
     9841 aacaggtttg gtggtaatta tgttatggtt caagtcgtgt gacttacttt caactgtggg
     9901 cgcttgatta ttcacattat taaattttat aaaagagaat ttaattggga taaaaggaat
     9961 taatccagca agcacagtta gataccaaac tttataattt aaactataat taaaatagcg
    10021 ttttaatatg tacctaaaaa atactaacaa tagaaatata aaacaaaagc ttactacact
    10081 cattattagt aatttagcca ttttgacacc ttctttcaaa tatttataat aaacaattga
    10141 catcaatatt acaattgtaa tattattgtt ttgtaaaaat tacaactgta atatcggagg
    10201 gtttattttg aaaaagttaa tatttttaat tgtaatggct ttagttttaa gtgcatgtaa
    10261 ttcaaacagt tcacatgcca aagagttaaa tgatttagaa aaaaaatata atgctcatat
    10321 tggtgtttat gctttagata ctaaaagtgg taaggaagta aaatttaatt cagataagag
    10381 atttgcctat gcttcgactt caaaagcgat aaatagtgct attttgttag aacaagtacc
    10441 ttataataag ttaaataaaa aagtacatat taacaaagat gatatagttg cttattctcc
    10501 tattttagaa aaatatgtag gaaaagacat cactttaaaa gaacttattg aggcttcaat
    10561 gacatatagt gataatacag caaacaataa aattataaaa gaaatcggtg gaatcaaaaa
    10621 agttaaacaa cgtctaaaag aactaggaga taaagtaaca aatccagtta gatatgagat
    10681 agaattaaat tactattcgc caaagagcaa aaaagatact tcaacgcctg ctgctttcgg
    10741 caagacttta aataaactta tcgcaaatgg aaaattaagc aaaaaaaata aaaatttctt
    10801 acttgattta atgtttaata ataaaaacgg agacacttta attaaagatg gtgttccaaa
    10861 agactataag gttgctgata aaagtggtca agcaataaca tatgcttcta gaaatgatgt
    10921 tgcttttgtt tatcctaagg gccaatctga acctattgtt ttagtcattt ttacgaataa
    10981 agacaataaa agtgataagc caaatgataa gttgataagt gaaaccgcca agagtgtaat
    11041 gaaggaattt taatattcta aatgcataat aaatactgat aacatcttat agtttgtatt
    11101 atattttcta ttatcgttga catgtataat tttgatatca aaacctgatt ttccctttat
    11161 tattttcgag atttattttc ttaattctct ttaacaaact agaaatattg tatatacaaa
    11221 aaatcataaa taatagatga atagtttaat tataggtgtt catcaatcga aaaagcaacg
    11281 tatcttattt aaagtgcgtt gcttttttct catttataag gttaaataat tctcatatat
    11341 caagcaaagt gacataattg ttgaatttat agtaactgaa acgtatgatt agggtgtata
    11401 ttaattatga gatggaaatc aatgattgtt ggctatgcga gagtatcatc gaacgatcaa
    11461 aatttagaaa gacaacttga aaatttaaag acgttttgtg ctgaaaaaat atttacagaa
    11521 aaacagtctg ggaaatctat tgaaaataga ccagtacttc aagaagcact taactttttg
    11581 aggatgggag atcggtttgt agtagaatcc attgattgtt taggtcgtaa ttatgatgaa
    11641 gtggttggca atccattact ggataagttt atgaaagatt taattattca aatattagca
    11701 atggtttcag aacaagagag aaatgaaagt aaacgtcgac aagcgcaagg aattcaagtt
    11761 gcgaaggaaa aaggcgtata taaagggcgc cccttacttt attcaccaaa tgctaaagat
    11821 ccacagaaac gtattattta tcatcgcgtt gtagaaatgt tagaagaagg ccaatcaatt
    11881 agtaaaattg cgaaagaagt aaatataaca aggcagacga tttacagaat taaaaacgat
    11941 aatgatttga tttaataaac tttgtttcaa tgcaactgag agcttctcac agcgtctcaa
    12001 aacagatgat tataatctat tatgagtttt taataataag acaagaaaag agagcgtaaa
    12061 tatgctctct ctttttctca ttaacgaacg tttataacgc aatcatgatg agagtattaa
    12121 tacagttaat tatttgaaat actttagttt atataatgtt gaattatagg tagtgaaatt
    12181 gtatttatta tacattgtca tatcgttgtt taatgatagt taatttaagg ataatagaat
    12241 taatattata tatagatgaa caaaacaagc aacagagcag aaaaaacact tttagattat
    12301 tttttactca ttctctattt tatttatttt tgctataata aataaagaat attaaatgga
    12361 ggtgttttta tgaaaaagaa atttgtttct agttgtattg ctagtacaat cttattcggc
    12421 actttattag gtgtaactta taaagctgaa gctgcaacag tacatgtagc tggtggtgtt
    12481 tggagccatg gtattggaaa acattacgta tggtcttatt atagtcacaa taaaagaaat
    12541 catggttcaa cagcagtagg gaaatattca tcttttagtg gtgttgctcg acctggtgtt
    12601 caatctaagg catctgctcc aaaggcttgg ggtggaaaca aaacatttta tagtcttcac
    12661 taattacata tattaattaa ggagaataga cagaattaag taaacttaat tctgtctatt
    12721 ctcttgaaag aggcaatatg aaaaaattca aattattttt agattttagt actttattat
    12781 taatttcagg gctattattt ttatttttct tcaaagaaaa tgaagaaatt atacctgaaa
    12841 gttccaatat tttaacaatc tctaattggg ataaatctaa cagtaaatca aaagtgttag
    12901 atgtcattga atcaggcgcc aaaaatcaaa atatacaaat aattaaatcc gttaaagatt
    12961 ttgataataa aaaagaattt tttgttttca actcaaaacg aaataattct gactttatac
    13021 gtaataaaac atcactttta actccatcag atttattaaa cagagagata aaaggaaagt
    13081 attatattat tggtgaacat ttcaatgtag aagagcttac gagtgaatta aaaaaagctg
    13141 gtttaacatc tgaagttgaa tacataaata gaaacatatt attttttgaa ttagtcatag
    13201 ataatgattt attagtgcct ttcatattct taggcgtatt atatcttttg tacttcttat
    13261 atgacagagg atataatttg aaattttatg ctattcaaaa gctacatggc ttttcaacta
    13321 cacgcattat ttttcataat attcatataa aaatcattta ttggctagta cttaccgcta
    13381 ttttctttat tgctaatata ttaatcattc atatagccaa tttagaattg gatttcttaa
    13441 tgtttattcg tagatttatc gtgttgcttt ttgtttttgc ttgtattact acattgctgt
    13501 ggctgatttc gtatagtctt cttttgaagt taagtattcc attaatctta aaagggaaaa
    13561 aaggatataa gttttcaata tttatgggta ccttcaccaa atgtatcatg gtgctaatac
    13621 tatctttttt actggcacaa aatttaaatg cttatacgaa gttaaagaat atatatgact
    13681 ctgaaaaaat ctggcaaaag ttagataatt actatacatt agagatttct cctaatatta
    13741 gaaattcaga agaaaaacaa attttagaaa ctaatatata caatttaatt aagaaaagcg
    13801 aacaacttgg tggattgctt ttaaagaata ataatatagc caatcccgat aaaaacaatt
    13861 acattcctga aaatggcaat gtctttttta tcaataacaa ttttctgaat ttctataaaa
    13921 atataaatca tgattttaaa atgattgaca atcattcgga taaaataaac gtgtttattc
    13981 ctccaaggtt acaatatcaa aaatctgaaa ttcaaaaaaa tcaccaagga tggattgact
    14041 ttcaaaagca acaaaacaaa aaagtagatg tgcaagtact gtcaaaaaac gtatctgttt
    14101 tttcttttga tcgcacaact aatttgaaat ttgggtatct agattctcca atagtaatgt
    14161 tgttgacact tgacgattta acaggagatt tttatttagc tgctgtttct caaggtggat
    14221 atctatttaa agatttagat acacttaaac agttgcttaa agataatcac ctagaagaag
    14281 aaattagcgg tataactaat tacaaggatt ctgtgttaaa tgaattaaat gaaacgaaaa
    14341 caaaaatgat aataacaata gttactatta ttattaacgt tttcattctt ttgatagcta
    14401 ctgtatttga aacaattcaa tattttagct ggaataaaaa gcaactatta ttaagaaaaa
    14461 ttcatggtta ttcattattc agcagtaatg tacgttattt aacaatttct atcttacttt
    14521 ctattatgtt agcttacact acgcatattt tatttggttc aaagatatta ctattcataa
    14581 taatgtctat tgctattatt caacatttac ttcaaattgc ttatattaaa tatcttgaaa
    14641 aatactttaa agatttaatg agggagattt aaacaatgaa aagaacttta attattgtaa
    14701 caattgtact attaatcatc atacttagtc tgttattggt tagaaatgag catttagatc
    14761 gttttaaccc actgttaaaa gaaaaagtat cttatgccaa agttccaaaa gatacccagg
    14821 actatagaaa tataactgta tactctaaaa atggagaaaa aaagagttat aaattggatt
    14881 ttttaggtta tgatcctaca aaagaatatg tgaaagtaaa tcataaaggg aaatatgtgc
    14941 gttcgataga atacattaca aaagatattc ctagcttttt aaaatgaggt gaccgatagt
    15001 gataaaactt gaaaatgttt ttgtaaaaaa aggtaataaa aacattttag acggatgtaa
    15061 ctttaatttt gaaaaaggaa aatcatatgc tttagttgga gaaagtggcg ctggaaaatc
    15121 tactttactt aacataatcg ctgggttcga agatgtatca caaggaagca tatatattga
    15181 agataagtta ctaaaaaaga aagtcgattt ttatagatat acgttagggt atttgtttca
    15241 aaattttgct ttacttgaaa atcaaacaat cagtcaaaat ttagatttag ccctaaagtt
    15301 caaaaagaat aaaaaagata atatgaactt aaagaaagaa gtattaaaaa aggtcggcct
    15361 agatttagat atcaagagaa ttgttagttc tctaagcggt ggtgaacaac aaagagtagc
    15421 attggcaaga ttaatattaa aagatccgaa aatcatttta gctgatgaac ctactgggtc
    15481 actagatacg aaaaatggaa aaatagttat tgatttatta ttgaagctac ttgatgaaaa
    15541 taaaactatg attgtcgtaa cgcatgattt agagcttgcg aaacgatttg atgtcatagt
    15601 caatatttct gagcttagaa gttaagaaat attgataggt atagccatac atagagaaaa
    15661 aacaatatga aatatgatta gtcattactt ttgagttttt taatttaagg actggatttt
    15721 ccagtccttt tttatgatga caaaaaacaa gcctcgcaga gcacacgttt ttaatgactt
    15781 atttggtttt taataagtct agtatgttaa aacttttctg tgtttaggtg ttataatcat
    15841 atgcagtgga agttgattta ttatgtctta ttcaattatt cgagttgtta aagtgaaatc
    15901 aaaaacaaat acaagaggta ttcaaagaca tattcaaaga gaaaataaaa attatgaaaa
    15961 tatagatatt gatttaagta agtcatattt aaactatgat ttagtcaatg atactaagtt
    16021 tgattttaat aaaaaaattg atgagaaaat cgaaaaaaat tataaaggga aacgcaaaat
    16081 tagaacagat gccattaaac atattgatgg attaattaca tcagataatg tgttttttaa
    16141 tcaattatct gaagaagaga caaaatagtt ttttaagcat gctaaagcgt tcttggaaga
    16201 agaatacggt aaagaaaatc ttttatatgc gacagtacat atggatgaga taacaccaca
    16261 tatgcattat ggcgtagttc ctattactaa agatggacgt ttaagtgcga aagaagttgt
    16321 aggtaataaa aaagcattaa ccgaattcca agatagattc aatacgtata ttaataagca
    16381 aggctatgat ttaaaacgag gaatttctag acaacttaca aaagaaaaac atgatcaagt
    16441 tagccgttat aaacaaaaaa cagaatatca taaacaaatg catatgcgct agaagcaaat
    16501 agaggattat ttaaagcaag aaaatgataa attgatacaa aactaccaaa aatcattaga
    16561 tacgcttaaa aagcttctga gtgtcatgta tgaaattaaa actaaaaatg taggtggatg
    16621 gtttcataaa gagaagcaag aaacaggcaa tattgtaatt actaaaactg attttgaaaa
    16681 atacactaaa caaattaaag cagcacagat gatattagat gattatgaat gtattaaaag
    16741 tgggaagtca ttaaaaaaag cagagaaaca aaatgaaagt ttagttaatg aattgactag
    16801 tgtccatatg gaaaatgaaa agctggttga agaatttaat gatttagcac aacgttataa
    16861 ttatttgttg tcagagaatg aaaagaaaga taaagaacta aattatacgc ttaagttatt
    16921 caatcaagtt tttaaaatca ttaaaagtat gatgaaagaa gagcgttatc atacactaat
    16981 aaatcatatt gataatcatt tagacaattc taaaatacgt gaagtaatga cgatagataa
    17041 caatgatgaa cagttcttta agaagaaata tcaagcacaa gaacgagaaa tcatttttaa
    17101 agaagataga gaagatggtt atacattata aaaatagata tttaaatgca tttgtttgag
    17161 gttaattatt ttttaggatg aaaaataggt agcccccctc gaacttttta atatattttt
    17221 ataaatcttt ttaaaaacct ttttaggggc ttgttcagcc ctagagcaac aagggatttc
    17281 aatgcctaaa ggagtgtttt tgccacataa aaggagtgtt tttgccacat aaaaggagtg
    17341 tttttgccac ataaaaggag tgtttttgcc acataaaagg agtgttttaa aaagttgtac
    17401 tccttttatg gtttgacatt gataataaag tagtttattg tgaataaaag ttttcaaatg
    17461 ggagtgtata ttcaaacgat actcctttag gttcgtaata aagggggctg tatcatgaca
    17521 ggagagacag ttgtatataa aaatgaaatg aatttagttc cattaaggcg atttacagct
    17581 actgaaatta atttattttt tgcgatgtgt aataaattaa aagagcaaga tactaataca
    17641 ttacgtttgt cttttgatga attaaaaaaa ttaagtaatt atagtccaga aacacgtaat
    17701 attaatagat ttgctaatga tttagataat gtgtataaaa agatgttgaa tttaaccatt
    17761 agatatgaag atgatgatgt aatagaaaga ttcgttttat tcaatcatta tagaatccat
    17821 aagcgagaac aatatctaga aatatcaact tcttcaaatt taaaacacat attgaattca
    17881 attactaata attttacaaa atttgaatta aaagaaatga ctaggcttaa atctacatat
    17941 tctaaaaata tgtttagatt acttaaacaa tacaaacata caggctattt aaaaattcac
    18001 atcgatgatt ttaaaaatcg tttagatata cctaaatctt atcgtatgac tgatattaac
    18061 aaaaatgttt ttaaacctat tattattgaa ttaggttcta tatttaataa tcttactatc
    18121 aataaaatca aagcgaaaaa agggcgtaaa attgaatgga tagagttcac atttgacgct
    18181 gagaaacgca ttcataacaa acgacaacct caaatgtcca agatagataa atcaagacaa
    18241 tatgtgaggc gtgaaaagac gcctaaatgg ttagaagaac ggtcatatga aaagcaacct
    18301 caaaaagatt acgacccaca attagagaaa gaacgagagg attttttaaa acaacttgaa
    18361 ctgaattggg aataacgcaa cttttcgttg cgagttggtt aactaaaagt atcgtgactt
    18421 ttcaattatt ttaattatag tttgagttaa ggagtgattt tatgaaatta gctgaagcta
    18481 taaaagaaca gagagaatta aaaggatggt ctcaagagga attagccaat atattaaagg
    18541 tttccagaca aagtgtttct aaatgggaaa gtgccaaaaa ctatccttcc ttagatattt
    18601 tgattgctat gagcgattta ttcggaatct ctttagagca tttaattaaa ggagatgcac
    18661 gttttaaaaa agttatttta gagggaaatt atagagagag taacagggta ccttccatag
    18721 ttgattttct ttatgatttt tggtggcttt tctttcctgt tgccggattt ttagtttggg
    18781 ccattcattc gttcatgggt tgattttaaa atcaaaattt aatgcacatc aggcaatatg
    18841 aaaaaggaaa gtatttttac gacaccttga aatgtattga taagaataga agagtttaca
    18901 aaaggcaata tgaaatatat actaaaagta acaagaattt cgtttgtaaa cgcaccaaac
    18961 cccgaactat tcgccgtagt tcggggtttt ttggtgaatt ggctttaatc gtctatatta
    19021 ttttttatca cgtttatgga gctaataacc aaataacgta acgagacaac cactgattgc
    19081 cgttgtcatg atgtgaacaa gaatttcatt cataaatcgc acctcctctc tacgccgaat
    19141 tgacatctga gagatagacg accttcacat tataacaaat ctctccttcc atgaaataac
    19201 atgaatatta ataaatgttg ttatatatta aagataaata aatataaggg tgggattgat
    19261 aatgtttggc gatcaaagac aagaagctac aaaatatgtc attaaagaag gttatcaaga
    19321 tatttatttc ctcaacaaaa acggggaatg gtattatttc gaagtaagaa gtgtctggcg
    19381 tggcaaacat attattagag ttaaagatgg tttgttaggt tggagaaaag aaatagtaac
    19441 tgaataaaga tacaaaatac ttgccaatgc aaaagaatga aaacgcgtca aatcaacgat
    19501 gtataaaaga tgcataaaag ataatgaaag taaaaagcca taaatgccgt catataggca
    19561 tttatggctt tttgtttggt taatataatc gatgattatc agaagttcaa ttcacataaa
    19621 aaagcacggc taatttatga acttgtcgta cctttttaat taaaattagt taccaaatag
    19681 acgactccaa aacccctttt tagcttccgt ttcttcactc ttttcttcta tatcactatc
    19741 attgtcattt ggattagctg aaacgtcttc tctttgtgct tcttcacctt gtgtactttc
    19801 gtcgctttga cttccagata tatccttata ttgaatctct gtttctttag tttcttcctg
    19861 atctcggtta atatttacag gactagtagt atatgttttt tcttgtgcat caacgttttg
    19921 tctagcgtta gtagatgtat caaagggata attaaggtgt ttttcttttt ctaattgatt
    19981 ttctaatttt tgaatctttt tattactctc taatgccaga acttgctgat tctcaagcaa
    20041 ttttgatctt tcttgcaact cttttctgta aaattcaatt gttgtttcat gacgactttc
    20101 ttgtttttca atttgattat ttaaagtatt aatctgattt ttaagtattt ctactatttg
    20161 agcatcgctt ttagattcaa aattaatatt atccgactct tttttttgtt tgtcttcagt
    20221 agcactttca aaaccatatt tctttttatt ttcatttact ctttgaatga ttttttctat
    20281 atcggtatct tctttaataa acgaagtgtt ttcctgtttt attgtctcta tattcaatct
    20341 cttaatatta ttaaaaatcg tttgcttact aacccctaat tcttcactca acctttttac
    20401 acttttcata tcatcaaccc tttataaaat gtttataaac ccttagtaaa atattataag
    20461 cttataacga ttaataataa agtaatcgac aaataaaaat ataaattcgc caaccttttt
    20521 atcaagggtt tgtaaagggt ttatggtgtt tatcaaaggt ttataaacct atattttccc
    20581 ttttttaagt ttattcaaag tgctataata accttaatat aacaaaaacc ccaactactg
    20641 gtaatagtcg gggt
//
