LOCUS       NZ_CP047772             2592 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-1 plasmid pEcl1-7, complete
            sequence.
ACCESSION   NZ_CP047772
VERSION     NZ_CP047772.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747507
            Assembly: GCF_015910385.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910385.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:57:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,699
            CDSs (total)                      :: 4,582
            Genes (coding)                    :: 4,458
            CDSs (with protein)               :: 4,458
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 34 of 124
            Pseudo Genes (incomplete)         :: 91 of 124
            Pseudo Genes (internal stop)      :: 17 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047772.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-1"
                     /isolation_source="Rectal"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl1-7"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2012-09-01"
                     /collected_by="Public Health Ontario"
     gene            1770..2072
                     /locus_tag="GVI62_RS23365"
                     /old_locus_tag="GVI62_23320"
     CDS             1770..2072
                     /locus_tag="GVI62_RS23365"
                     /old_locus_tag="GVI62_23320"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     gene            2059..>2592
                     /locus_tag="GVI62_RS23370"
                     /old_locus_tag="GVI62_23325"
                     /pseudo=""
     CDS             2059..>2592
                     /locus_tag="GVI62_RS23370"
                     /old_locus_tag="GVI62_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     Typing          4..749
                     /name="IncFII"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1056..1244)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.947"
                     /coverage="98.0"
                     /evalue="1.39e-59"
ORIGIN
        1 gtgaatgaca atcagatttc tgttcactgg tcagatctac cgaaagatga gctaacccgt
       61 ttctgccagg atgtcgatgc cggcactcag ggtaatttcc tcattgcacc ggtcaaaaaa
      121 ctgacccgcc gcaaacgcgg tgagcattcc acaaaagcca aatgtgagaa cccggcctgg
      181 tatcgcccgg agcattataa aaagctctcc ggacagctcg gtcatgccta caatcgcctg
      241 gtgaaaaaag acaaagaaac cgggcaggtc agcctgcgca tgcatgtttc tcgtcaccct
      301 ctctatgtcg ccggccgccg taaggcggga cgtaaatatg gcttccgtcc ggaacgtcag
      361 cgcctgctcg atgcgctctg gccagtgctc atcagcttct gtgatgccgg caagcatacc
      421 gtcggcatgt gtatctcccg tcttgcaaaa gaactcagtg caaaggacgc aaaaggcaat
      481 gtcattcctg aaacggaggt gacggtgtca cgcctttcac ggcttattga ggaacaggta
      541 cggtttggcg ttctgggcct cgcagaagaa cgcgcctggg atcgtgaatc ccgcacctgg
      601 ttgccaacgt atgtctatat aacccccgtg ggattccaga tgctgggcgt cgatatggac
      661 aagctgttta aagagcagga gaagaagctc cgtcagagcg ccgagcgtga gcagctgatc
      721 cgggaaggag tgatgagcga acatgatgat gttcaggccc actctgcgcg gaaatgctgg
      781 tctggccgta agcgtcggga ggcactcgtt taccgccgca ataaaggcgc ggagcgtaaa
      841 cgtgccaaca atctgattaa gctgccggca gatgaaccct caatgttcac gcgagattac
      901 gttaaacatg accgctgggt agagctttgg cgagagtgtt tgcgtgtgaa ttacgatccg
      961 aatgttgatg ttcgagcagt aaaaccccgc aagcctaagg acggagaatc attggcttct
     1021 gcaactgcgg aacttgtacg gggcgcagtg gctgaaaccc tgaaatattc cacgaagccg
     1081 gctgacatgg ttgctgatcc tgaatggttc ctcgaattga ctaagcagac acataagcgc
     1141 cgatttgtgg ctaccggtgg cgcactcaaa gacatactga aactcgatca ggaaactgat
     1201 gccgatatgg taatcgggga tgatattccc gaaggcgatg atgatggttc acgcattgct
     1261 tttgagtgga aaaccgaatc caaaaagtac cgtcgctcac cgtcaaaaga taaagctgaa
     1321 tcagactgac agggtgtcag ggctgcgccc cgacaccctg ccaacacctc acgcagtcag
     1381 attaaaatct gccagcgctc gctgtttccg aaatcgtcaa actctaaatc gtggggtccc
     1441 gctggcttgc cagtgggttc cgatttagtg attgacgaca ttgctgattg aaaaattggc
     1501 tgaggtggtt ggattcacga agtgaggcca accacctgga agacggtttt taattgtctg
     1561 gtaccggata caaccagaac gaatggagtt ggttctgtcg tgatgcgaaa aagtatgggt
     1621 tcgcaaatca cagactaaat agatttaccc aattccattc gagtgaggtt gttatgtttt
     1681 ttgaggttct taatcatgtt gatagtgcgt tgagcgttgc tgtttcgctc ctggcgttgt
     1741 ggcagttttt ggtggcgggt gggcgttgaa tggctggttc gatccgcgcg cgatccttgt
     1801 atttatactt aagggataac tctggcgata aaaaatggca tcaagccttg cgtaccaagg
     1861 cttcggggct tatttgcgtt gtagaaggtt ttggaagtat tctgttggga gtggtcgcct
     1921 taaaagcgac ccccgcaacg acggccatcg ttcgggggtg tgaccacctg cagaaattcg
     1981 attcgaagcg acaaccaaca ggtgatacgg caatgataac acgtcaaatg agccatcgcc
     2041 cagcatggga cggtgtgcat gcaaaaccct aaactccaga acctcacgga ttactccccc
     2101 tcagacgcgc cctgggacgc tcacaaaagc gtttctgacg acgtaggtgg gatttacctt
     2161 ctcgcagccg aatatgagcg ctacggggcg cgtatggctt cctgtggcgg tttactgagg
     2221 tttggttggt ctaccctcaa agaaactgga gaaactcgcc ttaggctacg tgaggcgcat
     2281 ttctgtcgtg tccggcactg tcctgtctgc cagtggaggc gatccctgat gtggcaagcc
     2341 cgtttttatc agtccctgcc gcgtattgtt gcggattatc cagatgcacg ctggatgttc
     2401 ctgaccctca cagtgcgaaa ctgcgcgatc ggggaattgg gtgagacact taaccgaatg
     2461 aacatggcct tccagcgact taaggatcgc aaagagtttc gacctgttca gggctggatc
     2521 cgcaccacgg aagttacacg tggaagtgac ggtagtgcgc acccgcactt tcacactctg
     2581 atgatggttc ct
//
