LOCUS       NZ_CP047771             2656 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-1 plasmid pEcl1-6, complete
            sequence.
ACCESSION   NZ_CP047771
VERSION     NZ_CP047771.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747507
            Assembly: GCF_015910385.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910385.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:57:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,699
            CDSs (total)                      :: 4,582
            Genes (coding)                    :: 4,458
            CDSs (with protein)               :: 4,458
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 34 of 124
            Pseudo Genes (incomplete)         :: 91 of 124
            Pseudo Genes (internal stop)      :: 17 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047771.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-1"
                     /isolation_source="Rectal"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl1-6"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2012-09-01"
                     /collected_by="Public Health Ontario"
     gene            1..>876
                     /gene="repA"
                     /locus_tag="GVI62_RS23345"
                     /old_locus_tag="GVI62_23305"
                     /pseudo=""
     CDS             1..>876
                     /gene="repA"
                     /locus_tag="GVI62_RS23345"
                     /old_locus_tag="GVI62_23305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /name="repA"
     gene            <880..1794
                     /locus_tag="GVI62_RS23350"
                     /old_locus_tag="GVI62_23310"
                     /pseudo=""
     CDS             <880..1794
                     /locus_tag="GVI62_RS23350"
                     /old_locus_tag="GVI62_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     Typing          4..749
                     /name="IncFII"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1521..1712)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.409"
                     /coverage="100.0"
                     /evalue="6.18e-88"
ORIGIN
        1 gtgaatgaca atcagatttc tgttcactgg tcagatctac cgaaagatga gctaacccgt
       61 ttctgccagg atgtcgatgc cggcactcag ggtaatttcc tcattgcacc ggtcaaaaaa
      121 ctgacccgcc gcaaacgcgg tgagcattcc acaaaagcca aatgtgagaa cccggcctgg
      181 tatcgcccgg agcattataa aaagctctcc ggacagctcg gtcatgccta caatcgcctg
      241 gtgaaaaaag acaaagaaac cgggcaggtc agcctgcgca tgcatgtttc tcgtcaccct
      301 ctctatgtcg ccggccgccg taaggcggga cgtaaatatg gcttccgtcc ggaacgtcag
      361 cgcctgctcg atgcgctctg gccagtgctc atcagcttct gtgatgccgg caagcatacc
      421 gtcggcatgt gtatctcccg tcttgcaaaa gaactcagtg caaaggacgc aaaaggcaat
      481 gtcattcctg aaacggaggt gacggtgtca cgcctttcac ggcttattga ggaacaggta
      541 cggtttggcg ttctgggcct cgcagaagaa cgcgcctggg atcgtgaatc ccgcacctgg
      601 ttgccaacgt atgtctatat aacccccgtg ggattccaga tgctgggcgt cgatatggac
      661 aagctgttta aagagcagga gaagaagctc cgtcagagcg ccgagcgtga gcagctgatc
      721 cgggaaggag tgatgagcga acatgatgat gttcaggccc actctgcgcg gaaatgctgg
      781 tctggccgta agcgtcggga ggcactcgtt taccgccgca ataaaggcgc ggagcgtaaa
      841 cgtgccaaca atctgattaa gctgccggca gatgaagttt ctgacgacgt tggagggatt
      901 tacctgcttg cggctgaata tgagcgctac ggggcgcgta tggcctcctg tggcggtttg
      961 ctgcggttcg ggtggtctac cctcaaagaa accggagaaa cgcgcctgag gctgcgtgag
     1021 gcgcatttct gccgtgtccg gcactgtcct gtctgccagt ggaggcgctc cctgatgtgg
     1081 caagcccgtt tctaccagtc cctgccgcgt atcgtggcgg attatcccga tgcccgctgg
     1141 atgttcctga ccctgactgt gcgtaactgt gcgatcgggg agctgggcga gacgctgaac
     1201 cggatgaacg caggtttcca gcgactgaag gatcgcaagg agttcagtcc agtgcagggc
     1261 tggatacgta ccacggaagt cacccgcggg agcgacggca gcgcacaccc gcactttcac
     1321 accctgatga tggtgccgcc atcgatgttg tctgggcgtg attacatcaa gcatgagcgc
     1381 tgggttgatc tgtggcgtgg gtgtcttcgg gtggattacg atccgaatgt cgatgtccgg
     1441 gcagtaaaac cccggaagcc gaaggacggc gaatcgctgg cctgcgcgac cgccgaactg
     1501 gtgcgcggcg cagtggccga aaccctgaaa tattccacaa agccggctga catggtggcc
     1561 gatcctgaat ggttcctcga actgacccgc cagacgcaca agcgccgatt tgtggcgacc
     1621 ggcggcgcac tcaaagacgt actgaagctc gatcaggaaa ccgacgcaga catggtgatc
     1681 ggggatgata tttccgaggg cgacgatgac ggttcccgca tcgcgtttga gtggaaaact
     1741 gaggtcagaa agtaccgtcg ctcaccgacg aaagataaaa ccgaatcaga gtaacggggt
     1801 gtcggggctg cgccctgacc atgaggactt tgggcatccg tcaccttggt ggcggtgtac
     1861 caaagtcaca tcttgtccga tagattttac tccgatgcag gatagggcta ccgccgcaaa
     1921 gtgacgccaa tcgcttactg agttcggaat ccgaacttag ggttttgcga tccgtaatac
     1981 ggattttttt aacgggacaa gatgtgtttt tggagtaccg ccatcacgat gccggtacca
     2041 aaaacgatcc gtcagggctg cgccccgaca ccctgccaac acctcacgcc gccagattga
     2101 aatctgtcag cgttcgccgt tactggaatc gtcaaacgct aaatcgtggg gtcccgctgg
     2161 cttgccagtg ggttccgatt tagtgattga cgactgcacc cataaaacac tgccggcagg
     2221 tggttgtgat cacgcagtga gcgcaaccac ctgataagcg tttttgggtt ttctggcggg
     2281 gcggatgtac ggggaaatta ggtgggaaat tcgcgcgcgt tccttgtatt tatacttaag
     2341 ggataactct gggattggaa ttcggcctca gcccttgtgc ggcaaggcct gagaggtgat
     2401 ttgcttggaa atgggtttgg aagtactctg tttggagtgg tcgcgttaaa agcgaccccc
     2461 gcaacgaggg caatcgttcg ggggcgtgac cacctgtagg aattctattt tgcgacagac
     2521 aacacaggtg atacggcaat gataacacgt caaatgagcc atcgcccagt atggggcggt
     2581 gtgcatgcaa aatcctaaac tccagaacct cacggattat tcccccgcag atgcgccctg
     2641 ggacgctcac agaagc
//
