LOCUS       NZ_CP047770             3193 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-1 plasmid pEcl1-5, complete
            sequence.
ACCESSION   NZ_CP047770
VERSION     NZ_CP047770.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747507
            Assembly: GCF_015910385.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910385.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:57:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,699
            CDSs (total)                      :: 4,582
            Genes (coding)                    :: 4,458
            CDSs (with protein)               :: 4,458
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 34 of 124
            Pseudo Genes (incomplete)         :: 91 of 124
            Pseudo Genes (internal stop)      :: 17 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047770.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-1"
                     /isolation_source="Rectal"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl1-5"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2012-09-01"
                     /collected_by="Public Health Ontario"
     gene            1..909
                     /gene="repA"
                     /locus_tag="GVI62_RS23325"
                     /old_locus_tag="GVI62_23285"
     CDS             1..909
                     /gene="repA"
                     /locus_tag="GVI62_RS23325"
                     /old_locus_tag="GVI62_23285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="WP_243457820.1"
                     /translation="MNDNQISVHWSDLPKDELTRFCQDVDAGTQGNFLIAPVKKLTRRK
                     RGEHSTKAKCENPAWYRPEHYKKLSGQLGHAYNRLVKKDKETGQVSLRMHVSRHPLYVA
                     GRRKAGRKYGFRPERQRLLDALWPVLISFCDAGKHTVGMCISRLAKELSAKDAKGNVIP
                     ETEVTVSRLSRLIEEQVRFGVLGLAEERAWDRESRTWLPTYVYITPVGFQMLGVDMDKL
                     FKEQEKKLRQSAEREQLIREGVMSEHDDVQAHSARKCWSGRKRREALVYRRNKGAERKR
                     ANNLIKLPADETNILSQYTLR"
                     /name="repA"
     gene            1180..1416
                     /locus_tag="GVI62_RS23330"
                     /old_locus_tag="GVI62_23290"
     CDS             1180..1416
                     /locus_tag="GVI62_RS23330"
                     /old_locus_tag="GVI62_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947370.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     gene            1705..2241
                     /locus_tag="GVI62_RS23335"
                     /old_locus_tag="GVI62_23295"
     CDS             1705..2241
                     /locus_tag="GVI62_RS23335"
                     /old_locus_tag="GVI62_23295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150440.1"
                     /translation="MKKVYTAIILIVLLCGGVLSANYIFLQRHMNEVLKEDPRNDGISV
                     WVYYKWFVNSSEINYDLRSVSAENSSLDVSRVMLQFAEKVKDYDFSKVYLSYRGKDKFY
                     LKGEYFKTLGQEYGIQNPVYTLRTIPENVYMLNGERAYSVWEGGLLGVMGKQMEDLSDF
                     SKAWYLDDFIKSMSD"
                     /name="hypothetical protein"
     gene            complement(2622..2735)
                     /locus_tag="GVI62_RS23685"
     CDS             complement(2622..2735)
                     /locus_tag="GVI62_RS23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          4..749
                     /name="IncFII"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2623..3106)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.213"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 gtgaatgaca atcagatttc tgttcactgg tcagatctac cgaaagatga gctaacccgt
       61 ttctgccagg atgtcgatgc cggcactcag ggtaatttcc tcattgcacc ggtcaaaaaa
      121 ctgacccgcc gcaaacgcgg tgagcattcc acaaaagcca aatgtgagaa cccggcctgg
      181 tatcgcccgg agcattataa aaagctctcc ggacagctcg gtcatgccta caatcgcctg
      241 gtgaaaaaag acaaagaaac cgggcaggtc agcctgcgca tgcatgtttc tcgtcaccct
      301 ctctatgtcg ccggccgccg taaggcggga cgtaaatatg gcttccgtcc ggaacgtcag
      361 cgcctgctcg atgcgctctg gccagtgctc atcagcttct gtgatgccgg caagcatacc
      421 gtcggcatgt gtatctcccg tcttgcaaaa gaactcagtg caaaggacgc aaaaggcaat
      481 gtcattcctg aaacggaggt gacggtgtca cgcctttcac ggcttattga ggaacaggta
      541 cggtttggcg ttctgggcct cgcagaagaa cgcgcctggg atcgtgaatc ccgcacctgg
      601 ttgccaacgt atgtctatat aacccccgtg ggattccaga tgctgggcgt cgatatggac
      661 aagctgttta aagagcagga gaagaagctc cgtcagagcg ccgagcgtga gcagctgatc
      721 cgggaaggag tgatgagcga acatgatgat gttcaggccc actctgcgcg gaaatgctgg
      781 tctggccgta agcgtcggga ggcactcgtt taccgccgca ataaaggcgc ggagcgtaaa
      841 cgtgccaaca atctgattaa gctgccggca gatgaaacta acatattaag ccagtataca
      901 ctccgctagc gctacgtgac tggtccggcg ctgcgcccgg aacccgcaaa ttcagaaact
      961 gaaagcggga caagatgtgt ttttggagta ccgccatcat gatgccggca ccaaaacgat
     1021 atctcagggc tgcgccccga taccccgcca acgcttcacg gcaccggatt aaaatccgtt
     1081 cccgttcacc gtttctcagg gccgttcccg gctgcaggtt cttcatgagg ttcgccgtgt
     1141 ctggcagact gtgcagagtt attctgaccg gagaacgcca tgaaacgact gataaacctg
     1201 acgcctgccg agaaaagatt tctcgacgat gctgttgccg ccgctgagag agcgtcaggc
     1261 aagaagctga atcagcccaa caggcacatt gtccttaaca gggctcgtgc acagatcgaa
     1321 tcgcagcgcc aggcagaacg gcagcgatct gcgcgtgagg aggagcggca gcaggcggaa
     1381 tttacctggt cgcgtccgcg ggcgccgcgt cgctgattgc cactttcgtc ggttttgtgg
     1441 ggagttcagg gcgaaacagc gtggtattca ctccggtgca tagtcattcg ccaccgtaaa
     1501 actcaccaga gcgattccgg gcggttttct gggggtaaga cgtgctcttt tgtggctgaa
     1561 ggcgcagaaa cgccctgagg ccgttttcgc ggtgcgcgta agaagacgtt atggtaaatc
     1621 gatgcttaat tccttgctaa cttatggcac cttagcttaa aaatctcagg gtaaacttta
     1681 gagcagttga gatggattat agaaatgaaa aaagtatata cagcaatcat attaatagta
     1741 ctcttatgtg gtggtgtttt atctgccaac tacatttttc ttcaaaggca catgaatgag
     1801 gttctgaaag aagatccaag gaatgatggt attagcgttt gggtttatta taagtggttt
     1861 gtaaactctt ccgagattaa ttatgacctt cgttctgttt ctgcagaaaa tagttcactg
     1921 gatgttagta gggtgatgct gcaatttgct gaaaaagtga aagattatga ttttagtaag
     1981 gtttatctta gttacagagg gaaagataag ttttacttaa aaggagagta ttttaaaact
     2041 cttgggcagg aatatggtat tcaaaacccc gtatatacat taagaacaat tccggaaaac
     2101 gtgtatatgt taaatggaga gagagcttat agtgtttggg agggtgggct tctgggtgta
     2161 atgggaaaac agatggaaga tcttagtgat ttctctaagg cttggtactt agatgatttc
     2221 attaaaagca tgagcgatta atttgaagcg atcagtatac attgacgatt agctcacgca
     2281 aatcgctcat tggtcactgg ggtgttttta ctcgtagatg ctttagatgt gtgacctgct
     2341 ccccgtggat tacaaggccg tcatctgata atgcccctaa tgggtcagat ctctaggata
     2401 gtagcaatac agcaaggaac ttcttgagat cctttctgtt gcgtcgtaat ctcttgctct
     2461 gtaaacgaaa aaaccgccca gcaaggcggt tttctcgaag gttctcagag cagcaactct
     2521 ttgaaccgag gtaactggct agttagagga gcgcagtcac caaaactgtt cgtttagttt
     2581 agccttaact ggcgcatgac ttcaagacta tctcctctaa atcagttacc agtggctgct
     2641 gccagtggcg cttttgcatg tcttaccggg ttggactcaa gacgatagtt accggataag
     2701 gcgcagcagt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
     2761 tacccggaac tgagtgtcag gcgtggaatg agataaacgc ggccataaca gcggaatgac
     2821 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccaccag ggggaaacgc
     2881 ctgggatctt taagtcctgt cgggtttcgc cgccactgat ttgagcgtca gattttttga
     2941 tgcttgtcag gggggcggag cctatggaaa aacggcagcg ccgcatcctt tttcgggccc
     3001 ttcttgacct cagcttcatt tcgttacctc gtcattcgtg agcaggtaac gcccgccgca
     3061 gtctcacggc aggagcgtag cgaccgagtg agcgaggagg cgtcatttca ccatcatcag
     3121 cattttgcac ttacgcaccg cagccgcatt tttcccctgc cacatgaagc acttccctga
     3181 gccaccacgc cca
//
