LOCUS       NZ_CP047769             3371 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-1 plasmid pEcl1-4, complete
            sequence.
ACCESSION   NZ_CP047769
VERSION     NZ_CP047769.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747507
            Assembly: GCF_015910385.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910385.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:57:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,699
            CDSs (total)                      :: 4,582
            Genes (coding)                    :: 4,458
            CDSs (with protein)               :: 4,458
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 34 of 124
            Pseudo Genes (incomplete)         :: 91 of 124
            Pseudo Genes (internal stop)      :: 17 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047769.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-1"
                     /isolation_source="Rectal"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl1-4"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2012-09-01"
                     /collected_by="Public Health Ontario"
     gene            1..>876
                     /gene="repA"
                     /locus_tag="GVI62_RS23305"
                     /old_locus_tag="GVI62_23260"
                     /pseudo=""
     CDS             1..>876
                     /gene="repA"
                     /locus_tag="GVI62_RS23305"
                     /old_locus_tag="GVI62_23260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /name="repA"
     gene            1659..1886
                     /locus_tag="GVI62_RS23310"
                     /old_locus_tag="GVI62_23270"
     CDS             1659..1886
                     /locus_tag="GVI62_RS23310"
                     /old_locus_tag="GVI62_23270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            2252..2437
                     /locus_tag="GVI62_RS23315"
                     /old_locus_tag="GVI62_23275"
     CDS             2252..2437
                     /locus_tag="GVI62_RS23315"
                     /old_locus_tag="GVI62_23275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2728..2946)
                     /locus_tag="GVI62_RS23320"
                     /old_locus_tag="GVI62_23280"
     CDS             complement(2728..2946)
                     /locus_tag="GVI62_RS23320"
                     /old_locus_tag="GVI62_23280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(2485..2572)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          4..749
                     /name="IncFII"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          918..1424
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.774"
                     /coverage="99.0"
                     /evalue="6.33e-132"
     Typing          3199..3371
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.857"
                     /coverage="99.0"
                     /evalue="7.14e-47"
ORIGIN
        1 gtgaatgaca atcagatttc tgttcactgg tcagatctac cgaaagatga gctaacccgt
       61 ttctgccagg atgtcgatgc cggcactcag ggtaatttcc tcattgcacc ggtcaaaaaa
      121 ctgacccgcc gcaaacgcgg tgagcattcc acaaaagcca aatgtgagaa cccggcctgg
      181 tatcgcccgg agcattataa aaagctctcc ggacagctcg gtcatgccta caatcgcctg
      241 gtgaaaaaag acaaagaaac cgggcaggtc agcctgcgca tgcatgtttc tcgtcaccct
      301 ctctatgtcg ccggccgccg taaggcggga cgtaaatatg gcttccgtcc ggaacgtcag
      361 cgcctgctcg atgcgctctg gccagtgctc atcagcttct gtgatgccgg caagcatacc
      421 gtcggcatgt gtatctcccg tcttgcaaaa gaactcagtg caaaggacgc aaaaggcaat
      481 gtcattcctg aaacggaggt gacggtgtca cgcctttcac ggcttattga ggaacaggta
      541 cggtttggcg ttctgggcct cgcagaagaa cgcgcctggg atcgtgaatc ccgcacctgg
      601 ttgccaacgt atgtctatat aacccccgtg ggattccaga tgctgggcgt cgatatggac
      661 aagctgttta aagagcagga gaagaagctc cgtcagagcg ccgagcgtga gcagctgatc
      721 cgggaaggag tgatgagcga acatgatgat gttcaggccc actctgcgcg gaaatgctgg
      781 tctggccgta agcgtcggga ggcactcgtt taccgccgca ataaaggcgc ggagcgtaaa
      841 cgtgccaaca atctgattaa gctgccggca gatgaagccc ccctgacaag catcacagaa
      901 tctgacgctc aaatcagtgg tggcgaaacc cgatgcggct taaagatacc aggcgtttcc
      961 ccctggtggc tccctcgtgc tcccccgcat tcctgccttt cggtttaccg gtgtcactcc
     1021 gctgttatgg ccgcggtttc tcattccacg cctgacactc agttccggga aggcagttcg
     1081 ctccaggatg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg
     1141 taactatcaa cttgagtcca acccggaaag acacgactaa tcgccattgg caacggccat
     1201 tggcaaacag gatgtgcgag agatgtctca agagttcttg aagtggcggc cttagtgagg
     1261 ctacactgaa aggacaaatt tggtgacatg tctcgcacaa gactgttacc acggttaggg
     1321 gatctgccag gattacctaa ccttcgaaaa accacctgcc agggtggttt tttcgtttac
     1381 agggcaagag attacgcgcg gaaaaaaagg atctcaagaa gatcctttat aaattagaca
     1441 tccaacctca caaagaggaa tacttaaatc atcttggtat atgtatcggc cggattacat
     1501 ctcatataag cctcctcccc gcctgacacc acactttgac catagcacct cgcgcctaac
     1561 tcgatataca gcccaccatc aagcctcttt tcacataaaa aatgacctat agttagatgg
     1621 ttagtgacca aaactgatat ggataaaagg agctggcttt gaaaaagatt tttgtttcag
     1681 tgttcgcagc ggcagttgcg ctttcagccc tcaccggctg cacgcgcaca agctatgcca
     1741 tacacaccaa tgacggtcgc actattgtca gcgacggcaa acctaaagaa tcagactcag
     1801 gcctgcttgg ctatacagac gcgaatggtg tcaaacagca gatcaataaa accgacgtta
     1861 aagaagtctc cgaaattccg cactgagcat cttctccgaa aaaggccgct aatgtggcct
     1921 ttttctttac ctgtggttct ccgccaaaat cccagcaaat tgcatcacca aagctaaaag
     1981 ctttcagggt tgtctaaaaa gcacaagaca taagaggaag tgcggtattt tataatcaag
     2041 cccccaagaa tttaccataa catccgttgc ccgcaccgcc tgagacgcgt tcagcgcgtt
     2101 cctgacgaaa ccatgacaaa aaccacaaca aaccaccccg gaacccgtca gaaacgcgcc
     2161 tgttaaattt taacggcatg catgactatg caccagaatg acgccatgct cttttcacgc
     2221 aaaaatcatc accagacggg gaaaatcacc agtgaccaga caggaatccg ccgccctcaa
     2281 tatggccaaa tttatccgcg cacagacact tctcctcctt gagcggctcg agcagatgga
     2341 tctggatgag gctgccggct gctgtgagca cctgcacgat caggccgaag cgctttacgc
     2401 catgctgaac gcacagatag gcgaggaaaa tgcgtgaaaa tcggtgaacg ggtgcgcaat
     2461 tcagtgcgcg gccgtgaggc gatggcgggg tgtcggggcg cagccctgac cagggtattt
     2521 gtgatgccgg cgcgtgcgcg gtattacaaa tgcacatcct gtcccggaac ggacaccggg
     2581 aaacagcaaa aaaaaccggg cggcacgccc ggaactcaat caagttagat tagattactc
     2641 tcactcgtcc ataacagcat catggaacga cgaccaccgt ccgtgacggc cgcctcgttt
     2701 aagtatggac agaaatacag aaaatgctca ggacgaaatg taatgaatgc gaacggattc
     2761 aagaaattcg agcatgacag tccttacggc cggttcggtt tcagacaaaa tctgccggta
     2821 tgcatccagc atcatggctc cggcatcccc tccggcacgc cgtagccaga ccgaaacaac
     2881 ggacacaagc aggtgtcgct catcatcact aagagtcatc agggctccgg aagaaaaacc
     2941 aaacatggct tacctactgt taacggcaac gcagaagaaa ggtttacagg gtagcgggca
     3001 gggatgaaaa aaaaaaaatt gcagtcaggc cggaacggct gctgaaccgg tacaggcggg
     3061 ttccgggcgc agcgccggac cagtcacgga gcactagcgg agtgtatact ggcttaagat
     3121 gtagcatggc agtaacacat agcattaatg ccacataaga caggcgcaga aagtgaaaga
     3181 aaacgcgtca ggagaaaatg tgatacagaa tatattccgc ttcctcgctc actgactcgc
     3241 tccgctcggt cgttcggctg cggcgagcgg gacgggctta cggatggatc agaaatttcc
     3301 tgggggatgc caggaagata cttaacgaag aaagcataag gccgcgccaa agccgttttt
     3361 ccataggctc c
//
