LOCUS       NZ_CP047768             3697 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-1 plasmid pEcl1-3, complete
            sequence.
ACCESSION   NZ_CP047768
VERSION     NZ_CP047768.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747507
            Assembly: GCF_015910385.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910385.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:57:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,699
            CDSs (total)                      :: 4,582
            Genes (coding)                    :: 4,458
            CDSs (with protein)               :: 4,458
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 34 of 124
            Pseudo Genes (incomplete)         :: 91 of 124
            Pseudo Genes (internal stop)      :: 17 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047768.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-1"
                     /isolation_source="Rectal"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl1-3"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2012-09-01"
                     /collected_by="Public Health Ontario"
     gene            1..>876
                     /gene="repA"
                     /locus_tag="GVI62_RS23290"
                     /old_locus_tag="GVI62_23245"
                     /pseudo=""
     CDS             1..>876
                     /gene="repA"
                     /locus_tag="GVI62_RS23290"
                     /old_locus_tag="GVI62_23245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /name="repA"
     gene            complement(942..1676)
                     /locus_tag="GVI62_RS23295"
                     /old_locus_tag="GVI62_23250"
     CDS             complement(942..1676)
                     /locus_tag="GVI62_RS23295"
                     /old_locus_tag="GVI62_23250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065807857.1"
                     /translation="MTIQEVKKTRVRTKTENDKLTEYKMNPFVFDKELKIETKTRNLTV
                     RKGTELVNKDNADESESYFSNIVQKKEVDKEEFIKLFTSQIKVYFDLTKTAHKIFLIIL
                     ALYQKEIGKDYVLLTCKKAQNVAKTLDFELSAPIFYRGIKELVEKKIIAKSVDKIVFYI
                     NPAVFFNGDRARFVTEVIKKKEEIEKKQFLEELETTSKNKDFETDDSVENLDLMIARLQ
                     KKKEELERKEKFGQWQDLEDAS"
                     /name="hypothetical protein"
     gene            2639..>3205
                     /gene="mobV"
                     /locus_tag="GVI62_RS23625"
                     /pseudo=""
     CDS             2639..>3205
                     /gene="mobV"
                     /locus_tag="GVI62_RS23625"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     Typing          4..749
                     /name="IncFII"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgaatgaca atcagatttc tgttcactgg tcagatctac cgaaagatga gctaacccgt
       61 ttctgccagg atgtcgatgc cggcactcag ggtaatttcc tcattgcacc ggtcaaaaaa
      121 ctgacccgcc gcaaacgcgg tgagcattcc acaaaagcca aatgtgagaa cccggcctgg
      181 tatcgcccgg agcattataa aaagctctcc ggacagctcg gtcatgccta caatcgcctg
      241 gtgaaaaaag acaaagaaac cgggcaggtc agcctgcgca tgcatgtttc tcgtcaccct
      301 ctctatgtcg ccggccgccg taaggcggga cgtaaatatg gcttccgtcc ggaacgtcag
      361 cgcctgctcg atgcgctctg gccagtgctc atcagcttct gtgatgccgg caagcatacc
      421 gtcggcatgt gtatctcccg tcttgcaaaa gaactcagtg caaaggacgc aaaaggcaat
      481 gtcattcctg aaacggaggt gacggtgtca cgcctttcac ggcttattga ggaacaggta
      541 cggtttggcg ttctgggcct cgcagaagaa cgcgcctggg atcgtgaatc ccgcacctgg
      601 ttgccaacgt atgtctatat aacccccgtg ggattccaga tgctgggcgt cgatatggac
      661 aagctgttta aagagcagga gaagaagctc cgtcagagcg ccgagcgtga gcagctgatc
      721 cgggaaggag tgatgagcga acatgatgat gttcaggccc actctgcgcg gaaatgctgg
      781 tctggccgta agcgtcggga ggcactcgtt taccgccgca ataaaggcgc ggagcgtaaa
      841 cgtgccaaca atctgattaa gctgccggca gatgaaacac caactgcaga tctaactacc
      901 tataaaagac ctaaatacaa ataaaaagcc catcagggct tttatgatgc atcttccaga
      961 tcctgccatt gcccgaattt ttcttttcgc tctaattctt cttttttctt ttgtaatctt
     1021 gcaatcatca gatctaaatt ttcaacacta tcatcagttt caaaatcttt attttttgat
     1081 gtagtttcta actcttcgag aaattgtttt ttctctatct cttctttttt cttgataact
     1141 tcagttacaa atcttgctct atcaccatta aaaaaaactg cagggtttat ataaaaaaca
     1201 attttatcta cagatttagc aataattttt ttctctacca gttcttttat acctcgataa
     1261 aaaattggtg ctgaaagctc aaaatctaat gttttagcaa cattctgagc tttcttacat
     1321 gtcaataaaa cataatcttt accgatttct ttttgatata aagctaaaat tataaggaaa
     1381 attttatgag ctgtttttgt tagatcaaaa tatactttta tttgagatgt aaataatttt
     1441 ataaattctt ctttatctac ttctttcttt tgaacaatat tagaaaaata agattctgat
     1501 tcgtcagcat tatctttatt tactaattca gtaccttttc taacagttaa atttcttgtt
     1561 tttgtttcaa tttttagttc tttgtcaaaa acaaaaggat tcatcttata ttctgttaat
     1621 ttatcatttt ctgtttttgt tctaactctt gtttttttta cttcttgtat tgtcataatc
     1681 tattcctctt taaatttaga tacttaatgc tagttttata actgatctca ttaagaagtc
     1741 aagtggaaac gataaaaaaa aatcgttttt tgcgacaaaa acgataaata aaattctaga
     1801 aattttagtg tgtttatcgt ttttatgggt gtaaaacgat aaagtttatt actctagagt
     1861 ataaaactta ttacttaatc gttacttttt taaggataga gtataaaact ttgtactttt
     1921 tgtttttttg aaaaaatggg gcaaagccag acgtgacaag gcttagagag gagttttagg
     1981 atttgagttt ttctgcaaga attaataaat attaagatct aagagtaaaa tcaaaatcta
     2041 aaaccgccct cgccgggcag gccaacccct cgaacccaca tacagcaagc tgtatgcagg
     2101 ttctcacggt taaagtaact acaaaaaccc gtcacagagc gttctacagc tactgggggg
     2161 aacccctaaa cccgttcccc ccaccagaac aagtactctt gttctgggtc cccccacccc
     2221 tgcgagtggt caagaacttg cccacatcgc aacccgtctt ttttgttcat gtattttatt
     2281 attattgtga tcaatccttt ttaaagtaaa agtttacttt tgctttaaag aggattcagc
     2341 aacgagccgt taggcgaggc ggtagctatt tgacagaata tattaaagtg ataactttaa
     2401 atatatacgt tgagtttact ttctcggtct atcactcact agaccgagct acgctcagtc
     2461 gttcgttggg gggcatagcc cacccaagcc ccgaaccggt ttgcaccggt tcaacgtcaa
     2521 aaccttacac agctgcacag gtggcattta acgccacatg agaatgtttc cgacattctc
     2581 atatattgga tttatccaaa aaaaatgggg gttgtataaa taatatggag aaataaaaat
     2641 gtattcaata atcagaaaac aatcacacaa aaaaacagct atacaagctg tacataatca
     2701 taatatgaga atagttatag aagaaaatgt aaattctaaa aaatcaaatt tgaatgaaat
     2761 attaatcggt actgaaaata ctagatccga cataatgagt tatattaaaa ataatgatat
     2821 tgtcattaga aataaagaca cagtaatttt taacgaattc gttttaactg catcaccaga
     2881 atttttcttt aacaataaag atggttcaaa gaaaactaga tctgaatata atgacaattt
     2941 gaaagattgg gttaaaaccc aaattgagtt tttggaaaaa gaaaattacg gaaaatgtgt
     3001 taatgcagtt ttgcatttag atgaaagtac cccacatatt catgcattaa ttttacctat
     3061 agttgacaac aaattaaaca acaaaagctt ttggcgtggt aaaaattcat atggaagatt
     3121 acaagatata tataatgcat caaatagtaa atttggttta aaacgtggtg aagaaaaaag
     3181 taaaactctt gttgatcata caacactaaa agattacaga gaattaataa aacaagattt
     3241 agaagaagaa aaagaatttt ataatgagtt atcgaaagat atattagaca ttccaccaag
     3301 aaaaaattta attggtttag aaaaaaaata ctctgctgaa gaagttaaag aaattgctac
     3361 agctactttt aaaaagctaa atagacaaag aagaagagca aaatttagag ctaaaaaatc
     3421 tgatgaaaaa gctgatgcag ctacaagtga attttataaa aaaaagaaag aaacatcgaa
     3481 aattgagttt ttttataatc aaaatatgca gcaattggaa aaaacctctg tggaaaattc
     3541 cacacttaaa acccagaact tggaacttaa gcaatcacaa agtgacctag atctggtgag
     3601 aaaattttta cctatggagc tgcaaagcct tgtcatcaga gccagagaac tggcctctgg
     3661 cggcgattct ggcggtacaa agcatactgc agaacct
//
