LOCUS       NZ_CP047767             8951 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-1 plasmid pEcl1-2, complete
            sequence.
ACCESSION   NZ_CP047767
VERSION     NZ_CP047767.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747507
            Assembly: GCF_015910385.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 8951)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8951)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910385.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 20:57:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,699
            CDSs (total)                      :: 4,582
            Genes (coding)                    :: 4,458
            CDSs (with protein)               :: 4,458
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 34 of 124
            Pseudo Genes (incomplete)         :: 91 of 124
            Pseudo Genes (internal stop)      :: 17 of 124
            Pseudo Genes (multiple problems)  :: 17 of 124
            Pseudo Genes (short protein)      :: 1 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047767.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8951
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-1"
                     /isolation_source="Rectal"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl1-2"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2012-09-01"
                     /collected_by="Public Health Ontario"
     gene            1..>876
                     /gene="repA"
                     /locus_tag="GVI62_RS23245"
                     /old_locus_tag="GVI62_23200"
                     /pseudo=""
     CDS             1..>876
                     /gene="repA"
                     /locus_tag="GVI62_RS23245"
                     /old_locus_tag="GVI62_23200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013087098.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /name="repA"
     gene            877..1716
                     /locus_tag="GVI62_RS23250"
                     /old_locus_tag="GVI62_23205"
     CDS             877..1716
                     /locus_tag="GVI62_RS23250"
                     /old_locus_tag="GVI62_23205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            1703..2554
                     /gene="repC"
                     /locus_tag="GVI62_RS23255"
                     /old_locus_tag="GVI62_23210"
     CDS             1703..2554
                     /gene="repC"
                     /locus_tag="GVI62_RS23255"
                     /old_locus_tag="GVI62_23210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            3001..4140
                     /locus_tag="GVI62_RS23260"
                     /old_locus_tag="GVI62_23215"
     CDS             3001..4140
                     /locus_tag="GVI62_RS23260"
                     /old_locus_tag="GVI62_23215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711650.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_265334573.1"
                     /translation="MMLIVADAINLGLTKMAESCPGTTYAKLSWLQAWHIRDETYSTAL
                     AELVNAQFRQPFAGNWGDGTTSSSDGQNFRTGSKAESTGHINPKYGSSPGRTFYTHISD
                     QYAPFSAKVVNVGIRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFGLMHLLGFRFA
                     PRIRDLGETKLFIPKGDAAYDALKPMISSDRLNIKQIRAHWDEILRLATSIKQGTVTAS
                     LMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALAR
                     AVFFYRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERATSALRGNGTALDDTLL
                     QYLSPLGWEHINLTGDYLWRSSAKVGAGKFRPLRPLPPA"
                     /name="Tn3 family transposase"
     gene            complement(4238..5017)
                     /locus_tag="GVI62_RS23265"
                     /old_locus_tag="GVI62_23220"
     CDS             complement(4238..5017)
                     /locus_tag="GVI62_RS23265"
                     /old_locus_tag="GVI62_23220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_058199817.1"
                     /translation="MELPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSST
                     LYTATTYSVSREAKMLSWLSEKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTDQE
                     LLAIYKEVLNLLNSIAIIDCPFISNIDHRLKESKFFIDNQLLDDIDQDDFDAELWGDHK
                     TYLSLWNELNETRVEERLVFSHGDITDSNIFIDKFNEIYFLDLGRAGLADEFVDISFVE
                     RCLREDASEETAKIFLKHLKNDRPDKRNYFLKLDELN"
                     /name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
     gene            complement(5829..6113)
                     /locus_tag="GVI62_RS23270"
                     /old_locus_tag="GVI62_23225"
     CDS             complement(5829..6113)
                     /locus_tag="GVI62_RS23270"
                     /old_locus_tag="GVI62_23225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            6312..>6953
                     /locus_tag="GVI62_RS23610"
                     /pseudo=""
     CDS             6312..>6953
                     /locus_tag="GVI62_RS23610"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            7060..7473
                     /locus_tag="GVI62_RS23615"
     CDS             7060..7473
                     /locus_tag="GVI62_RS23615"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            7572..8441
                     /locus_tag="GVI62_RS23620"
     CDS             7572..8441
                     /locus_tag="GVI62_RS23620"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            8502..8714
                     /locus_tag="GVI62_RS23280"
                     /old_locus_tag="GVI62_23235"
     CDS             8502..8714
                     /locus_tag="GVI62_RS23280"
                     /old_locus_tag="GVI62_23235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            8716..8922
                     /gene="urfF"
                     /locus_tag="GVI62_RS23285"
                     /old_locus_tag="GVI62_23240"
     CDS             8716..8922
                     /gene="urfF"
                     /locus_tag="GVI62_RS23285"
                     /old_locus_tag="GVI62_23240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     ARG             complement(4242..5017)
                     /name="aph(3')-VIa"
                     /gene="aph(3')-VIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-VIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.46"
                     /coverage="100.0"
     Typing          6068..6223
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          4..749
                     /name="IncFII"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          895..1716
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          6313..8438
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtgaatgaca atcagatttc tgttcactgg tcagatctac cgaaagatga gctaacccgt
       61 ttctgccagg atgtcgatgc cggcactcag ggtaatttcc tcattgcacc ggtcaaaaaa
      121 ctgacccgcc gcaaacgcgg tgagcattcc acaaaagcca aatgtgagaa cccggcctgg
      181 tatcgcccgg agcattataa aaagctctcc ggacagctcg gtcatgccta caatcgcctg
      241 gtgaaaaaag acaaagaaac cgggcaggtc agcctgcgca tgcatgtttc tcgtcaccct
      301 ctctatgtcg ccggccgccg taaggcggga cgtaaatatg gcttccgtcc ggaacgtcag
      361 cgcctgctcg atgcgctctg gccagtgctc atcagcttct gtgatgccgg caagcatacc
      421 gtcggcatgt gtatctcccg tcttgcaaaa gaactcagtg caaaggacgc aaaaggcaat
      481 gtcattcctg aaacggaggt gacggtgtca cgcctttcac ggcttattga ggaacaggta
      541 cggtttggcg ttctgggcct cgcagaagaa cgcgcctggg atcgtgaatc ccgcacctgg
      601 ttgccaacgt atgtctatat aacccccgtg ggattccaga tgctgggcgt cgatatggac
      661 aagctgttta aagagcagga gaagaagctc cgtcagagcg ccgagcgtga gcagctgatc
      721 cgggaaggag tgatgagcga acatgatgat gttcaggccc actctgcgcg gaaatgctgg
      781 tctggccgta agcgtcggga ggcactcgtt taccgccgca ataaaggcgc ggagcgtaaa
      841 cgtgccaaca atctgattaa gctgccggca gatgaaatgg ctacccataa gcctatcaat
      901 attctggagg cgttcgcagc agcgccgcca ccgctggact acgttttgcc caacatggtg
      961 gccggtacgg tcggggcgct ggtgtcgccc ggtggtgccg gtaaatccat gctggccctg
     1021 caactggccg cacagattgc aggcgggccg gatctgctgg aggtgggcga actgcccacc
     1081 ggcccggtga tctacctgcc cgccgaagac ccgcccaccg ccattcatca ccgcctgcac
     1141 gcccttgggg cgcacctcag cgccgaggaa cggcaagccg tggctgacgg cctgctgatc
     1201 cagccgctga tcggcagcct gcccaacatc atggccccgg agtggttcga cggcctcaag
     1261 cgcgccgccg agggccgccg cctgatggtg ctggacacgc tgcgccggtt ccacatcgag
     1321 gaagaaaacg ccagcggccc catggcccag gtcatcggtc gcatggaggc catcgccgcc
     1381 gataccgggt gctctatcgt gttcctgcac catgccagca agggcgcggc catgatgggc
     1441 gcaggcgacc agcagcaggc cagccggggc agctcggtac tggtcgataa catccgctgg
     1501 cagtcctacc tgtcgagcat gaccagcgcc gaggccgagg aatggggtgt ggacgacgac
     1561 cagcgccggt tcttcgtccg cttcggtgtg agcaaggcca actatggcgc accgttcgct
     1621 gatcggtggt tcaggcggca tgacggcggg gtgctcaagc ccgccgtgct ggagaggcag
     1681 cgcaagagca agggggtgcc ccgtggtgaa gcctaagaac aagcacagcc tcagccacgt
     1741 ccggcacgac ccggcgcact gtctggcccc cggcctgttc cgtgccctca agcggggcga
     1801 gcgcaagcgc agcaagctgg acgtgacgta tgactacggc gacggcaagc ggatcgagtt
     1861 cagcggcccg gagccgctgg gcgctgatga tctgcgcatc ctgcaagggc tggtggccat
     1921 ggctgggcct aatggcctag tgcttggccc ggaacccaag accgaaggcg gacggcagct
     1981 ccggctgttc ctggaaccca agtgggaggc cgtcaccgct gatgccatgg tggtcaaagg
     2041 tagctatcgg gcgctggcaa aggaaatcgg ggcagaggtc gatagtggtg gggcgctcaa
     2101 gcacatacag gactgcatcg agcgcctttg gaaggtatcc atcatcgccc agaatggccg
     2161 caagcggcag gggtttcggc tgctgtcgga gtacgccagc gacgaggcgg acgggcgcct
     2221 gtacgtggcc ctgaacccct tgatcgcgca ggccgtcatg ggtggcggcc agcatgtgcg
     2281 catcagcatg gacgaggtgc gggcgctgga cagcgaaacc gcccgcctgc tgcaccagcg
     2341 gctgtgtggc tggatcgacc ccggcaaaac cggcaaggct tccatagata ccttgtgcgg
     2401 ctatgtctgg ccgtcagagg ccagtggttc gaccatgcgc aagcgccgcc agcgggtgcg
     2461 cgaggcgttg ccggagctgg tcgcgctggg ctggacggta accgagttcg cggcgggcaa
     2521 gtacgacatc acccggccca aggcggcagg ctgacccccc tgtttaacaa tcacgccccc
     2581 ccggtaactg tcacgccccc cccggtaact gtcacgaacc cccccggtaa ctgtcacgcc
     2641 cccaaacctg caaacccagg aagggtggga gttggcgtgg tgttggaaaa atccatccat
     2701 gattatccaa gaataatcca ctaggcgcgg ttaacagcgc cctagtgggg cactgtttgc
     2761 ccttgcctaa aatgccggcc agaggccgga taattgctct atccgctgcg ctaggctgca
     2821 cacgcccccc caccgctgcg cggcaggggg gaaaggcggg caaagcccgc taaaccccac
     2881 accaaattct gcgggaatac gctggagcga ttttagccgc tttagcggcc ttttccctac
     2941 ccgaagggtg ggggcggtgt gcagccccga aggggccatt tagcggccat ttcagcggct
     3001 gtgatgctga tagtggccga cgcgatcaac ctgggtctga ccaaaatggc cgagtcctgc
     3061 cctggcacca cctacgccaa gctgtcttgg ctgcaagcct ggcacatccg cgatgaaacc
     3121 tattcgacgg cgctggccga gctggtgaat gcgcagtttc ggcaaccctt cgccggcaac
     3181 tggggtgacg gcaccacgtc atcgtcggac ggccagaact tcagaaccgg cagcaaagca
     3241 gaaagcactg gtcatatcaa cccgaagtat ggaagcagtc caggacggac tttctacacc
     3301 catatctccg accagtacgc gcccttcagt gccaaggtgg tcaacgtggg cattcgtgat
     3361 tcaacttacg tgcttgatgg cctgctgtac cacgagtcgg acttgcgcat cgaggaacac
     3421 tacaccgaca cggcaggctt caccgatcac gtgtttggct tgatgcattt gctgggattt
     3481 cgcttcgcgc cgcgtatccg tgacttgggc gaaaccaagc tattcatccc caagggcgat
     3541 gccgcctatg acgcgctcaa gccgatgatt agcagcgaca ggctgaacat caagcaaata
     3601 cgcgcccatt gggatgaaat tctgcggctg gccacctcca tcaagcaagg cacggtaacg
     3661 gcttcgctga tgctgcgcaa actcggcagc tacccgcgcc agaacggctt ggccgtggcg
     3721 ttgcgcgagc tggggcgcat cgagcgcacg ctgttcattt tggattggct gcaaagcgtg
     3781 gagctgcgcc gccgcgtcca tgcggggctg aataagggcg aggcgcgcaa cgcgctggcc
     3841 agggcggtct tcttctaccg attgggtgaa atccgcgacc gcagttttga gcagcagcgc
     3901 taccgggcca gcggcctcaa tctggtgacg gcggccatcg tgttgtggaa cacggtatat
     3961 ctggagcgtg ccaccagtgc tttgcgtggc aacggcacgg cgctggacga cacattgttg
     4021 caatatctgt cgccgctggg gtgggagcac atcaacctga ccggcgatta cctatggcgc
     4081 agcagcgcca aggtcggtgc ggggaagttt aggccattgc gaccgctgcc accggcttag
     4141 cgtgctttat tttccgtttt ctgagacgac ccctgcatct acggtccaaa gaatcgacgt
     4201 aaaatgacaa ttaagtattt ttagaaaatg cttggaatca attcaattca tcaagtttta
     4261 aaaaataatt ccttttgtca ggtctatcat tttttaaatg ctttaaaaat attttagcag
     4321 tttcctccga tgcatcctct cttaggcaac gttcaacaaa ggatatatct acaaactcat
     4381 ctgctaaccc agcacggcca agatctaaaa aataaatttc attgaattta tctataaaaa
     4441 tattactatc cgtgatatcg ccatgagaaa aaaccaatct ttcttcaaca cgagtctcat
     4501 ttaactcatt ccatagactt aggtaagttt tatggtctcc ccataattca gcgtcaaaat
     4561 catcttgatc tatatcatca aggagttggt tatcaataaa aaattttgac tcttttaacc
     4621 gatgatcaat gtttgaaata aatggacaat caataatagc aattgaattt aacagattga
     4681 gtacctcctt atagatagca agcaattctt ggtctgttaa aaaaagcgct gaaattggtt
     4741 ttgcattgat cgctttagtg atcatgaatt caaactgctc atcctgaaaa gtcatgatga
     4801 gttcaggcac ctttaacttc tcagagagcc aactcaacat tttcgcttca cgagaaacac
     4861 tgtatgtggt cgctgtatat aaagtgctag atcgcttaag aaaaaaagtt tcattatttc
     4921 gattaaaaga ataaacatcc gatggcgact gaccaatttt atttggctct aaaacgctgt
     4981 ttccaataaa ttgttgaata atattgggca attccatcaa gctttccttt tattcagtat
     5041 taaaaacccc gcaaatgcga ggcctagtaa atagatgatc ttaatttggt tcactgtagc
     5101 aaaaatatgg cttattgcct agaagaactt gcaaaattat tgtcccagcc gacaaaatct
     5161 agtttagttc agacttgtat taataataaa tatatgaaac attactactt tatttattat
     5221 taagccagca tataccgtgg gcacaatatc cactacgtta ctattgaccc aaggcatact
     5281 ggtaagggga aaccccttaa ccccaacccc tcacaaaaag ctgtgagggg taaaaaacaa
     5341 ccgcaagata gaggttccgt tgctagacat tgccagccag tgcccgtcac tcccgtacta
     5401 actgtcacga acccctgcaa taactgtcac gcccccctgc aataactgtc acgaacccct
     5461 gcaataactg tcacgccccc aaacctgcaa acccagcagg ggcgggggct ggcggggtgt
     5521 tggaaaaatc catccatgat tatctaagaa taatccacta ggcgcggtta tcagcgccct
     5581 tgtggggcgc tgctgccctt gcccaatatg cccggccaga ggccggatag ctggtctatt
     5641 cgctgcgcta ggctacacac cgccccaccg ctgcgcggca gggggaaagg cgggcaaagc
     5701 ccgctaaacc ccacaccaaa ccccgcagaa atacgctgga gcgcttttag ccgctttagc
     5761 ggcctttccc cctacccgaa gggtgggggc gcgtgtgcag ccccgcaggg cctgtctcgg
     5821 tcgatcattc agcccggctc atccttctgg cgtggcggca gaccgaacaa ggcgcggtcg
     5881 tggtcgcgtt caaggtacgc atccattgcc gccatgagcc gatcctccgg ccactcgctg
     5941 ctgttcacct tggccaaaat catggccccc accagcacct tgcgccttgt ttcgttcttg
     6001 cgctcttgct gctgttccct tgcccgcacc cgctgaattt cggcattgat tcgcgctcgt
     6061 tgttcttcga gcttggccag ccgatccgcc gccttgttgc tccccttaac catcttgaca
     6121 ccccattgtt aatgtgctgt ctcgtaggct atcatggagg cacagcggcg gcaatcccga
     6181 ccctactttg taggggaggg cgcacttacc ggtttctctt cgagaaactg gcctaacggc
     6241 cacccttcgg gcggtgcgct ctccgagggc cattgcatgg agccgaaaag caaaagcaac
     6301 agcgaggcag catggcgatt tatcacctta cggcgaaaac cggcagcagg tcgggcggcc
     6361 aatcggccag ggccaaggcc gactacatcc agcgcgaagg caagtatgcc cgcgacatgg
     6421 atgaagtctt gcacgccgaa tccgggcaca tgccggagtt cgtcgagcgg cccgccgact
     6481 actgggatgc tgccgacctg tatgaacgcg ccaatgggcg gctgttcaag gaggtcgaat
     6541 ttgccctgcc ggtcgagctg accctcgacc agcagaaggc gctggcgtcc gagttcgccc
     6601 agcacctgac cggtgccgag cgcctgccgt atacgctggc catccatgcc ggtggcggcg
     6661 agaacccgca ctgccacctg atgatctccg agcggatcaa tgacggcatc gagcggcccg
     6721 ccgctcagtg gttcaagcgg tacaacggca agaccccgga gaagggcggg gcacagaaga
     6781 ccgaagcgct caagcccaag gcatggcttg agcagacccg cgaggcatgg gccgaccatg
     6841 ccaaccgggc attagagcgg gctggccacg acgcccgcat tgaccacaga acacttgagg
     6901 cgcagggcat cgagcgcctg cccggtgttc acctggggcc gaacgtggtg gagatggaag
     6961 gccggggcat ccgcaccgac cgggcagacg tggccctgaa catcgacacc gccaacgccc
     7021 agatcatcga cttacaggaa taccgggagg caatagacca tgaacgcaat cgacagagtg
     7081 aagaaatcca gaggcatcaa cgagttagcg gagcagatcg aaccgctggc ccagagcatg
     7141 gcgacactgg ccgacgaagc ccggcaggtc atgagccaga cccagcaggc cagcgaggcg
     7201 caggcggcgg agtggctgaa agcccagcgc cagacagggg cggcatgggt ggagctggcc
     7261 aaagagttgc gggaggtagc cgccgaggtg agcagcgccg cgcagagcgc ccggagcgcg
     7321 tcgcgggggt ggcactggaa gctatggcta accgtgatgc tggcttccat gatgcctacg
     7381 gtggtgctgc tgatcgcatc gttgctcttg ctcgacctga cgccactgac aaccgaggac
     7441 ggctcgatct ggctgcgctt ggtggcccga tgaagaacga caggactttg caggccatag
     7501 gccgacagct caaggccatg ggctgtgagc gcttcgatat cggcgtcagg gacgccacca
     7561 ccggccagat gatgaaccgg gaatggtcag ccgccgaagt gctccagaac acgccatggc
     7621 tcaagcggat gaatgcccag ggcaatgacg tgtatatcag gcccgccgag caggagcggc
     7681 atggtctggt gctggtggac gacctcagcg agtttgacct ggatgacatg aaagccgagg
     7741 gccgggagcc tgccctggta gtggaaacca gcccgaagaa ctatcaggca tgggtcaagg
     7801 tggccgacgc cgcaggcggt gaacttcggg ggcagattgc ccggacgctg gccagcgagt
     7861 acgacgccga cccggccagc gccgacagcc gccactatgg ccgcttggcg ggcttcacca
     7921 accgcaagga caagcacacc acccgcgccg gttatcagcc gtgggtgctg ctgcgtgaat
     7981 ccaagggcaa gaccgccacc gctggcccgg cgctggtgca gcaggctggc cagcagatcg
     8041 agcaggccca gcggcagcag gagaaggccc gcaggctggc cagcctcgaa ctgcccgagc
     8101 ggcagcttag ccgccaccgg cgcacggcgc tggacgagta ccgcagcgag atggccgggc
     8161 tggtcaagcg cttcggtgat gacctcagca agtgcgactt tatcgccgcg cagaagctgg
     8221 ccagccgggg ccgcagtgcc gaggaaatcg gcaaggccat ggccgaggcc agcccagcgc
     8281 tggcagagcg caagcccggc cacgaagcgg attacatcga gcgcaccgtc agcaaggtca
     8341 tgggtctgcc cagcgtccag cttgcgcggg ccgagctggc acgggcaccg gcaccccgcc
     8401 agcgaggcat ggacaggggc gggccagatt tcagcatgta gtgctttcgt tggtactcac
     8461 gcctgttata ctatgagtac tcacgcacag aagggggttt tatggaatac gaaaaaagcg
     8521 cttcagggtc ggtctacctg atcaaaagtg acaagggcta ttggttgccc ggtggctttg
     8581 gttatacgtc aaacaaggcc gaggctggcc gcttttcagt cgctgatatg gccagcctta
     8641 accttgacgg ctgcaccttg tccttgttcc gcgaagacaa gcctttcggc cccggcaagt
     8701 ttctcggtga ctgatatgaa agaccaaaag gacaagcaga ccggcgacct gctggccagc
     8761 cctgacgctg tacgccaagc gcgatatgcc gagcgcatga aggccaaagg gatgcgtcag
     8821 cgcaagttct ggctgaccga cgacgaatac gaggcgctgc gcgagtgcct ggaagaactc
     8881 agagcggcgc agggcggggg tagtgacccc gccagcgcct aaccaccaac tgcctgcaaa
     8941 ggaggcaatc a
//
