LOCUS       NZ_CP047763             2295 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-2 plasmid pEcl2-6, complete
            sequence.
ACCESSION   NZ_CP047763
VERSION     NZ_CP047763.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747508
            Assembly: GCF_015910365.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910365.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:35:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,153
            CDSs (total)                      :: 5,037
            Genes (coding)                    :: 4,921
            CDSs (with protein)               :: 4,921
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 42 of 116
            Pseudo Genes (incomplete)         :: 75 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 17 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047763.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-2"
                     /isolation_source="urine"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl2-6"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2011-07-12"
                     /collected_by="Public Health Ontario"
     gene            112..1017
                     /gene="mobV"
                     /locus_tag="GVI63_RS25630"
                     /old_locus_tag="GVI63_25590"
     CDS             112..1017
                     /gene="mobV"
                     /locus_tag="GVI63_RS25630"
                     /old_locus_tag="GVI63_25590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657476.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_276513505.1"
                     /translation="MDPCYRERETPNADAERTPENHCSVSQSADEAMGKLRELLPEKRR
                     KDAVLAVEYVMTASPEWWKEATPRQQAEFFAHSEQWLEKKYGKDRVVAAVVHRDEATPH
                     LSAFVVPLTQDGRLSAKEFIGGRSKMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHY
                     YESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMAS
                     QSAQNAKRARELQKTMDSQQKRLQSVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQ
                     RVEREKQRQMRSRGMGGMER"
                     /name="mobV"
     gene            complement(1081..1515)
                     /locus_tag="GVI63_RS25635"
                     /old_locus_tag="GVI63_25595"
     CDS             complement(1081..1515)
                     /locus_tag="GVI63_RS25635"
                     /old_locus_tag="GVI63_25595"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     gene            2047..2172
                     /locus_tag="GVI63_RS25865"
                     /old_locus_tag="GVI63_25600"
     CDS             2047..2172
                     /locus_tag="GVI63_RS25865"
                     /old_locus_tag="GVI63_25600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_275564281.1"
                     /translation="MRSPDWLDGRQNEGVSMANYAIMRCKKLTGMGSVASALQHC"
                     /name="hypothetical protein"
     Typing          110..1014
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.026"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggggtgcct gacgcccggt tgaggcgtac ttcaactgga caccattcca gaaagaccaa
       61 gcatggcatg gcctgccgct gtcttaccgt gctttatttc ccgttttctc tatcgacccc
      121 tgctacaggg agcgtgagac gccgaatgct gacgctgaac ggacaccaga aaaccactgc
      181 tctgtgtctc agtcggcaga tgaggcgatg ggaaagctgc gggagctgtt gccggaaaaa
      241 cgccgtaaag atgcggtact ggctgttgaa tacgttatga cggcaagccc tgagtggtgg
      301 aaagaagcga caccgcggca gcaggcggag tttttcgccc attctgagca gtggctggag
      361 aagaaatacg ggaaggatcg tgttgtggct gcggtagttc accgggacga ggccaccccg
      421 catttgtcag cgttcgtggt gccattgacg caggatggac ggctgtccgc gaaagagttt
      481 attggcgggc ggtcaaagat gcgtgacgat cagagcacct acgctgaatc agtaaaaaaa
      541 ctgggtcttg agcgcggcat cgagggaagc cgcgccacgc atcagacagt tcagcactac
      601 tacgaatcga taaaccgtgg cacccgtagc caggtatcga tctcgccgga ggcgctggag
      661 ccgcgtgtat tgcggaaggg cattttcacc aaagacgtgg aagatcaggc agccatagcc
      721 aaacgtctaa gccacgcagt gaacgatggc tttgcaggaa ccatcgctat ggcctctcag
      781 agcgcccaga atgcaaaaag agcgcgcgag ctacagaaga ctatggattc gcagcagaaa
      841 cgcctacaga gcgttacaga gccttttaag gggctttcca gagagcagat gacagaaatc
      901 ctgatgatgg ctcagcggtt taagcagcaa aaccaggaga aggagaaaca gcagcgtgtt
      961 gaacgtgaga aacagcgaca gatgcgatcg cgtggcatgg gcggtatgga gcgttagttt
     1021 tgatgcgatg caggataggg ctaacgccgc aaaatgacgc catttgcggc attccagaga
     1081 tcaggttctg ccgccctgta taacgctgag gccgtttttc agtgcttttt tgtggttgct
     1141 gacagtacct ttccagccaa actgagggtt cagacggtaa ctgtaactgc gccctacttt
     1201 tgggccttga agaatcacgg ataggtcaat tagcgcctta accgaacggc ttacattggt
     1261 tttctgcata cctaaagcgt cggcaatatc ggcttgggca atttggatgt aattttcata
     1321 atcgagatca gctaacagca tcattaagac tttgaattgc tcccctgtga gggtcttagc
     1381 aaggatcttt aaagcgtctt ggttcatagt gaaatgcctc tcaaaagctg acttctgttt
     1441 gggtcggata actgcgacaa aactacctac ctcttcccct gtagcagtgt ctatctgagt
     1501 aattcggtta ctcatttaag aggctctccc aaaaaaatag ttatcaataa tgataacaac
     1561 gttatcaata atgataacta tcgtcaagcg agagccgcgc cccacaaggg ctgagagaag
     1621 gctctcttct gtatcttata gcgagaagct catatgcccg gatagcgaac cctattttca
     1681 tcggttcgtg cgtaagcatt tctatgcccg ttcaggtgat attccgcttc ctcgttcagg
     1741 ctgttcgttc ctcgctgcgc tgcggtacgc ctcagcctga cctgcggcga gcggttcctg
     1801 ctctttcaaa agcgatattc attgcctagt ttcgtcaagg gcgagcgtag cgagtgcatt
     1861 tcacccttga cgacgcacaa ataaaacagg cttggcagtg gtgggaggtg acgcggagcg
     1921 acgccttcca cctgccagac cgccgttgat tttgaccttc tggcagcaag ctgcccgaac
     1981 gattttgcgt agcaaagtct agtgagtaca ctatcaatct gatagtggcc cgccggaggc
     2041 atcgcaatgc gatcccccga ctggctggat ggacgccaga acgagggggt aagcatggcg
     2101 aattacgcga taatgcgctg caaaaagctg acgggaatgg gcagtgtggc cagcgctttg
     2161 cagcactgct aggggtcgtc tcagaattcg gaaaataaag cacgctagcg gttgatctgt
     2221 caggttgaag cctgagaggc cgagcgcaga tcgtcagaaa aggcgaaaaa cgatcctaat
     2281 ctgacgcaac atagg
//
