LOCUS       NZ_CP047762             5112 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-2 plasmid pEcl2-5, complete
            sequence.
ACCESSION   NZ_CP047762
VERSION     NZ_CP047762.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747508
            Assembly: GCF_015910365.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910365.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:35:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,153
            CDSs (total)                      :: 5,037
            Genes (coding)                    :: 4,921
            CDSs (with protein)               :: 4,921
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 116
            CDSs (without protein)            :: 116
            Pseudo Genes (ambiguous residues) :: 0 of 116
            Pseudo Genes (frameshifted)       :: 42 of 116
            Pseudo Genes (incomplete)         :: 75 of 116
            Pseudo Genes (internal stop)      :: 18 of 116
            Pseudo Genes (multiple problems)  :: 17 of 116
            Pseudo Genes (short protein)      :: 1 of 116
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047762.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-2"
                     /isolation_source="urine"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl2-5"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2011-07-12"
                     /collected_by="Public Health Ontario"
     gene            complement(235..438)
                     /locus_tag="GVI63_RS25605"
                     /old_locus_tag="GVI63_25565"
     CDS             complement(235..438)
                     /locus_tag="GVI63_RS25605"
                     /old_locus_tag="GVI63_25565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_197377787.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELGVDRENGK"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            854..967
                     /locus_tag="GVI63_RS25610"
                     /old_locus_tag="GVI63_25570"
     CDS             854..967
                     /locus_tag="GVI63_RS25610"
                     /old_locus_tag="GVI63_25570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(1204..1572)
                     /locus_tag="GVI63_RS25615"
                     /old_locus_tag="GVI63_25575"
     CDS             complement(1204..1572)
                     /locus_tag="GVI63_RS25615"
                     /old_locus_tag="GVI63_25575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045626380.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     gene            complement(1645..2943)
                     /locus_tag="GVI63_RS25620"
                     /old_locus_tag="GVI63_25580"
     CDS             complement(1645..2943)
                     /locus_tag="GVI63_RS25620"
                     /old_locus_tag="GVI63_25580"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_097414638.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGIIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            complement(4688..5110)
                     /locus_tag="GVI63_RS25625"
                     /old_locus_tag="GVI63_25585"
     CDS             complement(4688..5110)
                     /locus_tag="GVI63_RS25625"
                     /old_locus_tag="GVI63_25585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          560..967
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 gttaccatcc tgtaattggg gtcgtctcag aattcggaaa ataaagcacg ctagcggttg
       61 atctgtcagg ttgaagcctg agaggccgag cgcagatcgt cagaaaaggc gaaaaacgat
      121 cctaatctga cgcaacatag gtggggtgcc tgacgcccgg ttgaggcgta cttcaactgg
      181 acaccattcc agaaagacca agcatggcat ggcctgccgc tgtcttaccg tgctttattt
      241 cccgttttct ctatcgaccc ctaattcatc cagtctcgag gacagtgtac ggaaaagatg
      301 ctccgcatca tcgtggagtt tctcacaaag gtcggcctcg acatcgagag caagctcgtt
      361 aagcttctcc agcaggagca gagactgatt ctggatgaat ttagccatgt tcagcgctgt
      421 tttctgttgt ttcgtcataa gaagcctcag agcgtcagtg ctcagtgtaa aaccagtctc
      481 ttttccgctt cctcgctcac tcagtcgcta cgctccggtc gttcggctgc ggcgagcggc
      541 aagaccattc ttataaaagc tcgagaaaac cgagcgatca aggtaggagg aaaagaggag
      601 gccgcgcagc ggccgttttt ccataggctc cgcccccctg acaagcatca cagaaacgga
      661 cgctcaaatc agtggtggcg aaacccgaca ggactataaa gatacctatc atttctgata
      721 ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgc cattccgctg
      781 ttatggccgc gtttatctca ttccacgcca gacactcagt tccgggtagg cagttcgctc
      841 caagctggac tgtatgcacg aaccccccgt tcagtccgac tgctgcgcct tatccggtaa
      901 ctatcgtctt gagtccaacc cggaaagaca tgcaaaagcg ccactggcag cagccactgg
      961 taactgattt agaggagtta gtcttgaagt tatgcgccgg ttaaggctaa actgaaagaa
     1021 caagttttgg ttactgcgct cctccaagcc agttaccttg gttctaagag cgccaactct
     1081 tggaaccttc gaaaaaaccg cccgccaagg cggttttttc gtttacagag cgagagatta
     1141 cgacgagaac acagtgatct caagaagatc ttgcctcaac atacagtaca atcgtaacta
     1201 agcctatttc ctgctcttcc aaaaccgatg atccttatca aaaaaaacta tataaaacac
     1261 attatccttt aaaaaaccag caacccttct tttctggcct cctatatttt ttataacacc
     1321 ccagttctca tcaactgata tttcatcagg gcatttaaaa tcattaactg ctgaatctgg
     1381 aaatcgagtg tagttagtaa aaataccatc agagctgact tttgttatcc cattagctgt
     1441 aagataaaca agcttttcaa ttaaatctgg caaactgccg tcaaccctcc aagaatcaat
     1501 agactgacca atatcatcga cgttatgaaa atatctaaaa ctaacagaaa taaatctatc
     1561 tgtagttttc ataatgctct ttgaggattt tgtttcctct ttcctcgaag agctacgaag
     1621 accgcggcct ctctttgaca taaatcatcc ctcaggcata agtttttcaa attcatctaa
     1681 attatcaaac aatgggatag catcaaatgc accagataaa taatcatgcc catacgatga
     1741 ttttaaagtt cgctgcttct ctttatactc aaccaaagaa tagagatgac tacttcctga
     1801 ttttatatct ttatcaacaa accaaatctg gtcacgtcta aaacaatcag tgttcattag
     1861 attaacatcc tgaacagcag ctattatttg gctaagcccc tcgctttgcg catggaatat
     1921 tctaaacaaa aacttagaca aaagggtgtg aaacttagaa tcaaactcat caactaacaa
     1981 taagttaccg tttatcatgg cattgtagat tggaccaaga agataaacta tctttttagt
     2041 accctcagac tcaaaatcaa taggtactga aatctcaccg ctagctgttt ttttcactac
     2101 tctaagagaa aaaaccttct ggttttttgc aaaattttgt attgaactaa ataaattttt
     2161 taaattatca tcatctgttt ttatatttcc aaatggaaaa tcaggtgcga cctcatcgag
     2221 gtaaatatca ttaatctgga atgctggaag tatttttttt agccaagcct taaaatcctc
     2281 agactcattt attaacttca tggtaattgc tgtaaacatc ccttcattag aaccagaaag
     2341 gaatacgata ttgttaaacc aattaactac tcgttttgaa tgctcaccat taaaaccagc
     2401 aacaaccgaa acaaatggaa catcctccct tgttttttga ataatccctt ctttgataaa
     2461 aaggtttgac tcactaaaag ccgccttatt tacctcaata gaaagaccat ctctttcaaa
     2521 gagaatggtt tctcttgatt ttggtgtgta gtacaaccat tcttctttta ttttcccctg
     2581 aaaaatagac aatccatatc tatatttaac cccatctgaa ataaaagtca tttccatttc
     2641 agatggttta tttggtccat cctcatcaag caaaaacggt attacgttct ttgtaatgct
     2701 actttcaata gcttgcacgg aatgcaaaac aatatctttc attaaagaaa ttgttttcat
     2761 gagattagac ttcccgctag cgttagcccc aaatatcaga gcagatttca gtatccgaac
     2821 atcactcact gaaaccacgt tagccaagtt tatgtcgtct ttcgcggatg acaccgccaa
     2881 catgctcata ttttgagcat ccataaagga aaatgcgtta gaaagactaa aattaaccat
     2941 cataagaaca ccccttacgc catccttaaa caacaaaatg agtaaatatt gacaaaatca
     3001 ggacttaagg atagcataca agcgttctaa aacaacaggt gcgtttaata gatagcacgt
     3061 tatggaacac ttgttaaaaa aagctaaatg cagggggtct atgagatttt ttctatacca
     3121 aatggttcct ttagccgcag ttgtcagctc agcagtgcta gctttacact cggtttcagg
     3181 atggggatgg tttctctttg tagcatttat gctcagcgga gctgatacag tgaagaaaca
     3241 tctaggaaat gacgcatgaa aggagaaaaa cgacgttttt tgaagtaagt gggttagcct
     3301 gtaccaccag aactaacccg taacaaacca tacacaaatg cggcacttga gtaccacctc
     3361 aagagcttga taaagccagc cacaagattt gttggttgct acagtacaat tttgatagca
     3421 aaaatcaaca ctcatgtggc gaaaaatagg aaatgcacca tgcagacaga cgagtctcag
     3481 gcatacattt ttcgccctta tatcacagta aagggcaaac ggatcacccg tccaaacggt
     3541 gggatgttca aaattccaat taatcgtgaa aagaagaagt aagttgtgac tgcagcccta
     3601 acgggctgcc ccacatttga ttaaagagga tggttctatg tcagtttact gtgtctctta
     3661 tgacctgaat caagcaggtc aaaactacaa tgcactatat gatgagttaa aaaaatcgcc
     3721 ggggtggtgc caccctttag attccacatg gctaatatca accggagaaa cagcgcaaca
     3781 actgtcggat cgtttgcgta ggcatctgga caataatgac accttgcttg tgattggtgt
     3841 aaccaaagaa tacgcaggat ggctcaccca agacacatgg gattggatga gaacagatat
     3901 atagatataa agccctgcgg ggcttttttt acgtctgtag ctggtgtgga tttaacataa
     3961 ccccctaatt gtacgcaccg ctaaaacggc ctcagggcgt ttctgacggg aaaccggtaa
     4021 aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt tttacggcat
     4081 gcatgagtat gcacgcaagt gcatagcgcg aaaattcacg ccattcagcc agggtaagtc
     4141 ctgtttccgg cgttcttccc atccgaaaac ggtgaacggg gacgcaattt attgcgtggc
     4201 cgtgaagcgt tgccggcgca gccggaaaac cggttttcag cacgttcaga aatcgggttc
     4261 gtcgacggcc ggcaatcacg ttatggcaag tcgccggacg actcaccaca cgattcaccg
     4321 gcgaatcttc actagagtcg tacccagagt cgccgagtct aaaaaacaac caaacgggga
     4381 gtcgtgtcta gagtcgggtc gttattaact tattgttttt atagtcctga gtcgttaccg
     4441 actctatgac gactcgccgg ggactctatg acgactcgtc atagagtcgg atctatataa
     4501 ataaaattta aaaaacgaaa tttcaggaaa gtaacccact gatattaaat acaaatctgc
     4561 aaaatgttaa ttttgcgtgg ggtgtgggta tttttagtgg tgaggagcgt tcagagatct
     4621 gacgattttt gggaaggttc aggaactccc cgggatggcc ggagaggaaa acggaagcat
     4681 gacggggtca ggattttttc cagaatttcc accacgggga atggggtggt gaagaaggtt
     4741 cgtgcttgtg ctccagaagc tgcatagcct gacgaaggga atcaatatgc tcatctcgag
     4801 ccttaacagc ccctttaaga ccatcgtttt cagcttcaag taaagcgatt ctatgttgta
     4861 aatcatgtaa accaacgctg ttaacacctg tagattctgc gtcacttttt acatcatatt
     4921 ttacagtatc gatgacacca tcaagagaac caaacactct aataagctct gaagtatcaa
     4981 taactttaat agccttaccg tgctttacaa cagaggtgta acttaacttc ccactgttaa
     5041 tcatcttgta aattgtgggg cgactaacct taacaagccg tgccgcttca ctaatagaca
     5101 ctctggccat ca
//
