LOCUS       NZ_CP047748             2295 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-4 plasmid pEcl4-8, complete
            sequence.
ACCESSION   NZ_CP047748
VERSION     NZ_CP047748.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747510
            Assembly: GCF_015910285.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910285.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:53:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,435
            CDSs (total)                      :: 5,321
            Genes (coding)                    :: 5,181
            CDSs (with protein)               :: 5,181
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 56 of 140
            Pseudo Genes (incomplete)         :: 93 of 140
            Pseudo Genes (internal stop)      :: 19 of 140
            Pseudo Genes (multiple problems)  :: 26 of 140
            Pseudo Genes (short protein)      :: 1 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047748.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-4"
                     /isolation_source="urine"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl4-8"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2012-02-18"
                     /collected_by="Public Health Ontario"
     gene            complement(join(2225..2295,1..763))
                     /gene="mobV"
                     /locus_tag="GVI65_RS27035"
                     /old_locus_tag="GVI65_27010"
     CDS             complement(join(2225..2295,1..763))
                     /gene="mobV"
                     /locus_tag="GVI65_RS27035"
                     /old_locus_tag="GVI65_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010657476.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_243459175.1"
                     /translation="MSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWKEATPR
                     QQAEFFAHSEQWLEKKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRS
                     KMRDDQSTYAESVKKLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVL
                     RKGIFTKDVEDQAAIAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDSQQKRLQ
                     SVTEPFKGLSREQMTEILMMAQRFKQQNQEKEKQQRVEREKQRQMRSRGMGGMER"
                     /name="mobV"
     gene            complement(1070..1195)
                     /locus_tag="GVI65_RS27265"
                     /old_locus_tag="GVI65_27015"
     CDS             complement(1070..1195)
                     /locus_tag="GVI65_RS27265"
                     /old_locus_tag="GVI65_27015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_275564281.1"
                     /translation="MRSPDWLDGRQNEGVSMANYAIMRCKKLTGMGSVASALQHC"
                     /name="hypothetical protein"
     gene            1727..2161
                     /locus_tag="GVI65_RS27045"
                     /old_locus_tag="GVI65_27020"
     CDS             1727..2161
                     /locus_tag="GVI65_RS27045"
                     /old_locus_tag="GVI65_27020"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(36..838)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.642"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 tctccttctc ctggttttgc tgcttaaacc gctgagccat catcaggatt tctgtcatct
       61 gctctctgga aagcccctta aaaggctctg taacgctctg taggcgtttc tgctgcgaat
      121 ccatagtctt ctgtagctcg cgcgctcttt ttgcattctg ggcgctctga gaggccatag
      181 cgatggttcc tgcaaagcca tcgttcactg cgtggcttag acgtttggct atggctgcct
      241 gatcttccac gtctttggtg aaaatgccct tccgcaatac acgcggctcc agcgcctccg
      301 gcgagatcga tacctggcta cgggtgccac ggtttatcga ttcgtagtag tgctgaactg
      361 tctgatgcgt ggcgcggctt ccctcgatgc cgcgctcaag acccagtttt tttactgatt
      421 cagcgtaggt gctctgatcg tcacgcatct ttgaccgccc gccaataaac tctttcgcgg
      481 acagccgtcc atcctgcgtc aatggcacca cgaacgctga caaatgcggg gtggcctcgt
      541 cccggtgaac taccgcagcc acaacacgat ccttcccgta tttcttctcc agccactgct
      601 cagaatgggc gaaaaactcc gcctgctgcc gcggtgtcgc ttctttccac cactcagggc
      661 ttgccgtcat aacgtattca acagccagta ccgcatcttt acggcgtttt tccggcaaca
      721 gctcccgcag ctttcccatc gcctcatctg ccgactgaga cacagagcag tggttttctg
      781 gtgtccgttc agcgtcagca ttcggcgtct cacgctccct gtagcagggg tcgatagaga
      841 aaacgggaaa taaagcacgg taagacagcg gcaggccatg ccatgcttgg tctttctgga
      901 atggtgtcca gttgaagtac gcctcaaccg ggcgtcaggc accccaccta tgttgcgtca
      961 gattaggatc gtttttcgcc ttttctgacg atctgcgctc ggcctctcag gcttcaacct
     1021 gacagatcaa ccgctagcgt gctttatttt ccgaattctg agacgacccc tagcagtgct
     1081 gcaaagcgct ggccacactg cccattcccg tcagcttttt gcagcgcatt atcgcgtaat
     1141 tcgccatgct taccccctcg ttctggcgtc catccagcca gtcgggggat cgcattgcga
     1201 tgcctccggc gggccactat cagattgata gtgtactcac tagactttgc tacgcaaaat
     1261 cgttcgggca gcttgctgcc agaaggtcaa aatcaacggc ggtctggcag gtggaaggcg
     1321 tcgctccgcg tcacctccca ccactgccaa gcctgtttta tttgtgcgtc gtcaagggtg
     1381 aaatgcactc gctacgctcg cccttgacga aactaggcaa tgaatatcgc ttttgaaaga
     1441 gcaggaaccg ctcgccgcag gtcaggctga ggcgtaccgc agcgcagcga ggaacgaaca
     1501 gcctgaacga ggaagcggaa tatcacctga acgggcatag aaatgcttac gcacgaaccg
     1561 atgaaaatag ggttcgctat ccgggcatat gagcttctcg ctataagata cagaagagag
     1621 ccttctctca gcccttgtgg ggcgcggctc tcgcttgacg atagttatca ttattgataa
     1681 cgttgttatc attattgata actatttttt tgggagagcc tcttaaatga gtaaccgaat
     1741 tactcagata gacactgcta caggggaaga ggtaggtagt tttgtcgcag ttatccgacc
     1801 caaacagaag tcagcttttg agaggcattt cactatgaac caagacgctt taaagatcct
     1861 tgctaagacc ctcacagggg agcaattcaa agtcttaatg atgctgttag ctgatctcga
     1921 ttatgaaaat tacatccaaa ttgcccaagc cgatattgcc gacgctttag gtatgcagaa
     1981 aaccaatgta agccgttcgg ttaaggcgct aattgaccta tccgtgattc ttcaaggccc
     2041 aaaagtaggg cgcagttaca gttaccgtct gaaccctcag tttggctgga aaggtactgt
     2101 cagcaaccac aaaaaagcac tgaaaaacgg cctcagcgtt atacagggcg gcagaacctg
     2161 atctctggaa tgccgcaaat ggcgtcattt tgcggcgtta gccctatcct gcatcgcatc
     2221 aaaactaacg ctccataccg cccatgccac gcgatcgcat ctgtcgctgt ttctcacgtt
     2281 caacacgctg ctgtt
//
