LOCUS       NZ_CP047747             5112 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-4 plasmid pEcl4-7, complete
            sequence.
ACCESSION   NZ_CP047747
VERSION     NZ_CP047747.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747510
            Assembly: GCF_015910285.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910285.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:53:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,435
            CDSs (total)                      :: 5,321
            Genes (coding)                    :: 5,181
            CDSs (with protein)               :: 5,181
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 56 of 140
            Pseudo Genes (incomplete)         :: 93 of 140
            Pseudo Genes (internal stop)      :: 19 of 140
            Pseudo Genes (multiple problems)  :: 26 of 140
            Pseudo Genes (short protein)      :: 1 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047747.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-4"
                     /isolation_source="urine"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl4-7"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2012-02-18"
                     /collected_by="Public Health Ontario"
     gene            complement(1692..2114)
                     /locus_tag="GVI65_RS27010"
                     /old_locus_tag="GVI65_26985"
     CDS             complement(1692..2114)
                     /locus_tag="GVI65_RS27010"
                     /old_locus_tag="GVI65_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2351..2554)
                     /locus_tag="GVI65_RS27015"
                     /old_locus_tag="GVI65_26990"
     CDS             complement(2351..2554)
                     /locus_tag="GVI65_RS27015"
                     /old_locus_tag="GVI65_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_197377787.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELGVDRENGK"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2970..3083
                     /locus_tag="GVI65_RS27020"
                     /old_locus_tag="GVI65_26995"
     CDS             2970..3083
                     /locus_tag="GVI65_RS27020"
                     /old_locus_tag="GVI65_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(3320..3688)
                     /locus_tag="GVI65_RS27025"
                     /old_locus_tag="GVI65_27000"
     CDS             complement(3320..3688)
                     /locus_tag="GVI65_RS27025"
                     /old_locus_tag="GVI65_27000"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045626380.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     gene            complement(3761..5059)
                     /locus_tag="GVI65_RS27030"
                     /old_locus_tag="GVI65_27005"
     CDS             complement(3761..5059)
                     /locus_tag="GVI65_RS27030"
                     /old_locus_tag="GVI65_27005"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_097414638.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGIIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     Typing          2676..3083
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 atcaggactt aaggatagca tacaagcgtt ctaaaacaac aggtgcgttt aatagatagc
       61 acgttatgga acacttgtta aaaaaagcta aatgcagggg gtctatgaga ttttttctat
      121 accaaatggt tcctttagcc gcagttgtca gctcagcagt gctagcttta cactcggttt
      181 caggatgggg atggtttctc tttgtagcat ttatgctcag cggagctgat acagtgaaga
      241 aacatctagg aaatgacgca tgaaaggaga aaaacgacgt tttttgaagt aagtgggtta
      301 gcctgtacca ccagaactaa cccgtaacaa accatacaca aatgcggcac ttgagtacca
      361 cctcaagagc ttgataaagc cagccacaag atttgttggt tgctacagta caattttgat
      421 agcaaaaatc aacactcatg tggcgaaaaa taggaaatgc accatgcaga cagacgagtc
      481 tcaggcatac atttttcgcc cttatatcac agtaaagggc aaacggatca cccgtccaaa
      541 cggtgggatg ttcaaaattc caattaatcg tgaaaagaag aagtaagttg tgactgcagc
      601 cctaacgggc tgccccacat ttgattaaag aggatggttc tatgtcagtt tactgtgtct
      661 cttatgacct gaatcaagca ggtcaaaact acaatgcact atatgatgag ttaaaaaaat
      721 cgccggggtg gtgccaccct ttagattcca catggctaat atcaaccgga gaaacagcgc
      781 aacaactgtc ggatcgtttg cgtaggcatc tggacaataa tgacaccttg cttgtgattg
      841 gtgtaaccaa agaatacgca ggatggctca cccaagacac atgggattgg atgagaacag
      901 atatatagat ataaagccct gcggggcttt ttttacgtct gtagctggtg tggatttaac
      961 ataaccccct aattgtacgc accgctaaaa cggcctcagg gcgtttctga cgggaaaccg
     1021 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     1081 gcatgcatga gtatgcacgc aagtgcatag cgcgaaaatt cacgccattc agccagggta
     1141 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     1201 tggccgtgaa gcgttgccgg cgcagccgga aaaccggttt tcagcacgtt cagaaatcgg
     1261 gttcgtcgac ggccggcaat cacgttatgg caagtcgccg gacgactcac cacacgattc
     1321 accggcgaat cttcactaga gtcgtaccca gagtcgccga gtctaaaaaa caaccaaacg
     1381 gggagtcgtg tctagagtcg ggtcgttatt aacttattgt ttttatagtc ctgagtcgtt
     1441 accgactcta tgacgactcg ccggggactc tatgacgact cgtcatagag tcggatctat
     1501 ataaataaaa tttaaaaaac gaaatttcag gaaagtaacc cactgatatt aaatacaaat
     1561 ctgcaaaatg ttaattttgc gtggggtgtg ggtattttta gtggtgagga gcgttcagag
     1621 atctgacgat ttttgggaag gttcaggaac tccccgggat ggccggagag gaaaacggaa
     1681 gcatgacggg gtcaggattt tttccagaat ttccaccacg gggaatgggg tggtgaagaa
     1741 ggttcgtgct tgtgctccag aagctgcata gcctgacgaa gggaatcaat atgctcatct
     1801 cgagccttaa cagccccttt aagaccatcg ttttcagctt caagtaaagc gattctatgt
     1861 tgtaaatcat gtaaaccaac gctgttaaca cctgtagatt ctgcgtcact ttttacatca
     1921 tattttacag tatcgatgac accatcaaga gaaccaaaca ctctaataag ctctgaagta
     1981 tcaataactt taatagcctt accgtgcttt acaacagagg tgtaacttaa cttcccactg
     2041 ttaatcatct tgtaaattgt ggggcgacta accttaacaa gccgtgccgc ttcactaata
     2101 gacactctgg ccatcagtta ccatcctgta attggggtcg tctcagaatt cggaaaataa
     2161 agcacgctag cggttgatct gtcaggttga agcctgagag gccgagcgca gatcgtcaga
     2221 aaaggcgaaa aacgatccta atctgacgca acataggtgg ggtgcctgac gcccggttga
     2281 ggcgtacttc aactggacac cattccagaa agaccaagca tggcatggcc tgccgctgtc
     2341 ttaccgtgct ttatttcccg ttttctctat cgacccctaa ttcatccagt ctcgaggaca
     2401 gtgtacggaa aagatgctcc gcatcatcgt ggagtttctc acaaaggtcg gcctcgacat
     2461 cgagagcaag ctcgttaagc ttctccagca ggagcagaga ctgattctgg atgaatttag
     2521 ccatgttcag cgctgttttc tgttgtttcg tcataagaag cctcagagcg tcagtgctca
     2581 gtgtaaaacc agtctctttt ccgcttcctc gctcactcag tcgctacgct ccggtcgttc
     2641 ggctgcggcg agcggcaaga ccattcttat aaaagctcga gaaaaccgag cgatcaaggt
     2701 aggaggaaaa gaggaggccg cgcagcggcc gtttttccat aggctccgcc cccctgacaa
     2761 gcatcacaga aacggacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     2821 cctatcattt ctgataggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     2881 cggtgccatt ccgctgttat ggccgcgttt atctcattcc acgccagaca ctcagttccg
     2941 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct
     3001 gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcgccac
     3061 tggcagcagc cactggtaac tgatttagag gagttagtct tgaagttatg cgccggttaa
     3121 ggctaaactg aaagaacaag ttttggttac tgcgctcctc caagccagtt accttggttc
     3181 taagagcgcc aactcttgga accttcgaaa aaaccgcccg ccaaggcggt tttttcgttt
     3241 acagagcgag agattacgac gagaacacag tgatctcaag aagatcttgc ctcaacatac
     3301 agtacaatcg taactaagcc tatttcctgc tcttccaaaa ccgatgatcc ttatcaaaaa
     3361 aaactatata aaacacatta tcctttaaaa aaccagcaac ccttcttttc tggcctccta
     3421 tattttttat aacaccccag ttctcatcaa ctgatatttc atcagggcat ttaaaatcat
     3481 taactgctga atctggaaat cgagtgtagt tagtaaaaat accatcagag ctgacttttg
     3541 ttatcccatt agctgtaaga taaacaagct tttcaattaa atctggcaaa ctgccgtcaa
     3601 ccctccaaga atcaatagac tgaccaatat catcgacgtt atgaaaatat ctaaaactaa
     3661 cagaaataaa tctatctgta gttttcataa tgctctttga ggattttgtt tcctctttcc
     3721 tcgaagagct acgaagaccg cggcctctct ttgacataaa tcatccctca ggcataagtt
     3781 tttcaaattc atctaaatta tcaaacaatg ggatagcatc aaatgcacca gataaataat
     3841 catgcccata cgatgatttt aaagttcgct gcttctcttt atactcaacc aaagaataga
     3901 gatgactact tcctgatttt atatctttat caacaaacca aatctggtca cgtctaaaac
     3961 aatcagtgtt cattagatta acatcctgaa cagcagctat tatttggcta agcccctcgc
     4021 tttgcgcatg gaatattcta aacaaaaact tagacaaaag ggtgtgaaac ttagaatcaa
     4081 actcatcaac taacaataag ttaccgttta tcatggcatt gtagattgga ccaagaagat
     4141 aaactatctt tttagtaccc tcagactcaa aatcaatagg tactgaaatc tcaccgctag
     4201 ctgttttttt cactactcta agagaaaaaa ccttctggtt ttttgcaaaa ttttgtattg
     4261 aactaaataa attttttaaa ttatcatcat ctgtttttat atttccaaat ggaaaatcag
     4321 gtgcgacctc atcgaggtaa atatcattaa tctggaatgc tggaagtatt ttttttagcc
     4381 aagccttaaa atcctcagac tcatttatta acttcatggt aattgctgta aacatccctt
     4441 cattagaacc agaaaggaat acgatattgt taaaccaatt aactactcgt tttgaatgct
     4501 caccattaaa accagcaaca accgaaacaa atggaacatc ctcccttgtt ttttgaataa
     4561 tcccttcttt gataaaaagg tttgactcac taaaagccgc cttatttacc tcaatagaaa
     4621 gaccatctct ttcaaagaga atggtttctc ttgattttgg tgtgtagtac aaccattctt
     4681 cttttatttt cccctgaaaa atagacaatc catatctata tttaacccca tctgaaataa
     4741 aagtcatttc catttcagat ggtttatttg gtccatcctc atcaagcaaa aacggtatta
     4801 cgttctttgt aatgctactt tcaatagctt gcacggaatg caaaacaata tctttcatta
     4861 aagaaattgt tttcatgaga ttagacttcc cgctagcgtt agccccaaat atcagagcag
     4921 atttcagtat ccgaacatca ctcactgaaa ccacgttagc caagtttatg tcgtctttcg
     4981 cggatgacac cgccaacatg ctcatatttt gagcatccat aaaggaaaat gcgttagaaa
     5041 gactaaaatt aaccatcata agaacacccc ttacgccatc cttaaacaac aaaatgagta
     5101 aatattgaca aa
//
