LOCUS       NZ_CP047746             6486 bp    DNA     circular CON 22-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-4 plasmid pEcl4-6, complete
            sequence.
ACCESSION   NZ_CP047746
VERSION     NZ_CP047746.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747510
            Assembly: GCF_015910285.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910285.1-RS_2024_04_22
            Annotation Date                   :: 04/22/2024 04:53:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,435
            CDSs (total)                      :: 5,321
            Genes (coding)                    :: 5,181
            CDSs (with protein)               :: 5,181
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 56 of 140
            Pseudo Genes (incomplete)         :: 93 of 140
            Pseudo Genes (internal stop)      :: 19 of 140
            Pseudo Genes (multiple problems)  :: 26 of 140
            Pseudo Genes (short protein)      :: 1 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047746.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-4"
                     /isolation_source="urine"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl4-6"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2012-02-18"
                     /collected_by="Public Health Ontario"
     gene            join(6361..6486,1..120)
                     /locus_tag="GVI65_RS27235"
     CDS             join(6361..6486,1..120)
                     /locus_tag="GVI65_RS27235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023343077.1"
                     /translation="MQPQPAVHDGLPGINARTVGAVVIRLETLASQQLRVRDQEVQLQP
                     PLILVLNPQHAVLVFVESGHQNPFKARHQLFALPGR"
                     /name="hypothetical protein"
     gene            complement(302..625)
                     /locus_tag="GVI65_RS26965"
                     /old_locus_tag="GVI65_26940"
     CDS             complement(302..625)
                     /locus_tag="GVI65_RS26965"
                     /old_locus_tag="GVI65_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001445156.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1269..1379
                     /locus_tag="GVI65_RS27240"
     CDS             1269..1379
                     /locus_tag="GVI65_RS27240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048246078.1"
                     /translation="MHDPPVQSDYHAHSGNYQLESNPERHDKIAIGGSHW"
                     /name="hypothetical protein"
     gene            complement(1634..2095)
                     /locus_tag="GVI65_RS26975"
                     /old_locus_tag="GVI65_26945"
     CDS             complement(1634..2095)
                     /locus_tag="GVI65_RS26975"
                     /old_locus_tag="GVI65_26945"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_065707752.1"
                     /translation="MVNTYYGYLLGVTLGEWAMGSDVQWQIDNGYLAQVMGDFSTESPS
                     FETPMFGRYFDTFQAAIHVAKAFPGSTLQPSEYEDKTGMLIRHYYVDCHPYYDRLPPEK
                     SDNFQYDEYPSAIHVAACIDQASKELDELLFGGGYGENGEGHASPIILY"
                     /name="hypothetical protein"
     gene            complement(2178..2828)
                     /locus_tag="GVI65_RS26980"
                     /old_locus_tag="GVI65_26950"
     CDS             complement(2178..2828)
                     /locus_tag="GVI65_RS26980"
                     /old_locus_tag="GVI65_26950"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_197389800.1"
                     /translation="MAWPAAVLPCFISRFLYRPLMPSLSVERWLALATAGDSQARFHLG
                     KIFSCGLGVHQDYKQAIYWFQLAAEQGHDDAQFSLGWAYHLGLGGYKDAQKASEWYQKA
                     AEQGHAEAQFQLGRLYAFSDLRNDALAVEWFLCAAKQDHSSAQFKLGWMYHLGMGVEQS
                     DRQAFHWFILAAEQDHAGAQASLGEMYKNGWGVEQDDVAAQDWFYRARTMHYP"
                     /name="tetratricopeptide repeat protein"
     gene            complement(3022..3558)
                     /locus_tag="GVI65_RS26985"
                     /old_locus_tag="GVI65_26955"
     CDS             complement(3022..3558)
                     /locus_tag="GVI65_RS26985"
                     /old_locus_tag="GVI65_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072159713.1"
                     /translation="MLDTDCITVLTARGITEILNSGGSQAWRLDATHVAKLPYLVCVQN
                     SKKDWGSQEAKHHHAFMIGKISGVSRAPENPKRWIINIDSYAEIDIPGQWDGNRNPVSY
                     RKLEDMGIDISKVDFQPLPKEDSLVLNTIKGGDEYDEYEEDSNRVRPINLKEAKAGLAL
                     YFGVNEDDIQITIKG"
                     /name="hypothetical protein"
     gene            complement(4221..5135)
                     /gene="lpxO"
                     /locus_tag="GVI65_RS26990"
                     /old_locus_tag="GVI65_26960"
     CDS             complement(4221..5135)
                     /gene="lpxO"
                     /locus_tag="GVI65_RS26990"
                     /old_locus_tag="GVI65_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728425.1"
                     /GO_process="GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A hydroxylase LpxO"
                     /protein_id="WP_023343074.1"
                     /translation="MLSLIIIFVFLISVLYAHSRGKIKQKFSRQLFDHSTFMAPINMFM
                     TGFSKLPSKQAWFPPETFPELQPLIDNWQVIRDEAVNLQGHIKSSEKNNDAGFNTFFKR
                     GWKRFYLKWYGDAHPSAERLCPVTTQLVSSIPCIKAAMFAELPSGSHLGKHRDPYAGSV
                     RYHLGLSTPNDERCFIEVDGVRRSWHDGEVIIFDETFVHWAQNDTEQTRIILFCDIERP
                     MKWRWSQKINRWIGGVLMSAAASPNDNNDRTGAINHLFKYVNAIRDAGQKLKKRNRKLY
                     YLIKNGVISLIFVWLAVSVFRWL"
                     /name="lpxO"
     gene            complement(5406..5621)
                     /locus_tag="GVI65_RS26995"
                     /old_locus_tag="GVI65_26965"
     CDS             complement(5406..5621)
                     /locus_tag="GVI65_RS26995"
                     /old_locus_tag="GVI65_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_023343075.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSSG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5625..6110)
                     /locus_tag="GVI65_RS27000"
                     /old_locus_tag="GVI65_26970"
     CDS             complement(5625..6110)
                     /locus_tag="GVI65_RS27000"
                     /old_locus_tag="GVI65_26970"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023343076.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASNAAILWWQSQQIL
                     DNYVSIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     Typing          complement(727..913)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.911"
                     /coverage="87.0"
                     /evalue="2.69e-52"
     Typing          893..1379
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.959"
                     /coverage="93.0"
                     /evalue="3.59e-159"
     Typing          complement(306..625)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="1.63e-64"
     Typing          complement(5309..6486)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.633"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctccagccgc cccttatcct ggtgctcaac ccacagcacg ctgtactggt ctttgtcgag
       61 tccgggcatc agaacccgtt caaagctcgc catcagcttt tcgcgctgcc cgggcggtaa
      121 atccttttcg gcaaaggaca ggacgccgga tgtgtacttt tttgcgtagg gtgaggcgtc
      181 gataagctcc ctgacttctt ccggcttccc ctgcagaacg gtggcgccct cgcgctgtcg
      241 gtctttcccc agcagataat ccaccggacc ggcgccaccg ccgcgccctc gcgggtgaaa
      301 cttaacgatc atcgtctgca cccttttcca gcacggcatg ccgcagccgc tcgagtccgg
      361 catctatggc catcagcgcg gcgacgacct gcacgcggtc gtgcccgctg ccaccaccgg
      421 cgttaacctg ccgggcaatc tggttaaggt tgttccccat gccggcaagc tgacgaagca
      481 gcgccggcga gatcgaggga agcttgccgg cgcgggcggg tttctcgtcc aggcacgtct
      541 ggcgcatcca ggctgcaagc tgtttaccgt cgcagcgctc cagcagacgc cggtgctcat
      601 cctcggtcac ccacatcgtg agcattttgc tgcgtttgtc tgccaatccg gcctcctgat
      661 aacgccggga cgggcgggaa atcttttccc ggtcggcgcg gtgttggaca agccgcaggc
      721 gcgtcagtgg ttttagcggg tttcggggcg cagccctgaa ccagtcacgt agcgctagcg
      781 gagtgtatac tggcttaata tggtaaatcg aagtgggagt tcagggaagt gcttcatgtg
      841 gcaggtaaaa aatgcggctg tggggcgtaa gtgcgaaatg ctggggacga tgaaatgacg
      901 cctcctcgct cactcggtcg ctacgctcct gccgtgagac tgcggcgggc gttaccggct
      961 cacgaatggc gggataaaga gatggcgctc aaatcaggaa gggctgggaa aaggatgcgg
     1021 cgttgccgtt tttccatagg ctccgccccc ctgacaagca tcacgaaata tgacgctcaa
     1081 atcagtggcg gcgaaacccg acaggactat aaagatccca ggcgtttccc cctggtagct
     1141 ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatgtg
     1201 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     1261 tggactgtat gcacgacccc cccgttcagt ccgactacca cgcccattcc ggtaactatc
     1321 aacttgagtc caacccggaa agacacgaca aaatcgccat tggcggtagc cactggtaag
     1381 caggatgtgc gagagatgta tcgagagttc ttgaagtggt ggccggacta cggctacact
     1441 ggaaggacag atttggtgac acgtctcgca caagactgtt accacggttc agaagttccc
     1501 caacttactg aaccttcgaa aaaccacctc cccaggtggt tttttcgttt tcagagcaag
     1561 agattacgcg cagagcaaaa cgatctcaag aagatcattt tacaaattgg ataatatccc
     1621 tccgccccta acactaatat aaaattatcg ggcttgcatg cccctcacca ttttccccat
     1681 atccaccacc aaacaatagt tcgtcgagtt ctttgctagc ttgatcaata cacgcagcca
     1741 catgaatagc agaagggtac tcatcatact gaaaattatc gcttttttca ggagggagtc
     1801 gatcataata cggatgacaa tccacataat aatgacgtat tagcatccca gttttatctt
     1861 cgtactctga tggctgcaac gtactccctg ggaaagcttt tgcaacatgt atagcagctt
     1921 gaaaggtatc aaaatatcgc ccaaacattg gcgtttcaaa gctaggcgac tctgtcgaaa
     1981 aatcccccat aacttgcgcc aaataaccat tatctatctg ccattgaaca tctgatccca
     2041 tagcccactc tcccagagtt actcctaata aataaccata atatgtattt accataacgc
     2101 ttcatgttat gaatcagcag cagccagctc cgcaagcctc ttacacgtag gcgtaaaaaa
     2161 acccgcacag ggcgggttta gggatagtgc atggttctgg cacgataaaa ccaatcctgc
     2221 gcagcaacat catcttgctc gaccccccat ccatttttat acatctcacc aagagatgcc
     2281 tgagctcctg catgatcttg ctcagcggcc agaataaacc aatggaaagc ctgcctatca
     2341 ctttgctcaa cgcccatacc gagatggtac atccagccaa gtttaaattg ggcactactg
     2401 tggtcttgct tcgcagcaca gagaaaccat tcgactgcta acgcatcatt tcttaaatca
     2461 gagaaagcat acagccttcc aagctgaaac tgagcctctg catgaccttg ctcagcagct
     2521 ttctgatacc attccgaagc tttttgagca tctttatatc cacctaaccc cagatgatat
     2581 gcccaaccaa gactaaactg ggcatcatca tggccttgct cagctgcaag ctgaaaccag
     2641 tatatagcct gcttataatc ctgatgcaca ccaagaccgc atgagaatat cttacccaga
     2701 tgaaatctag cctggctatc tccagcagta gctagtgcta accagcgctc aacagagagt
     2761 gacggcataa ggggtcgata gagaaaacgg gaaataaagc acggtaagac agcggcaggc
     2821 catgccatgc ttggtctttc tggaatggtg tccagttgaa gtacgcctca accgggcgtc
     2881 aggcacccca cctatgttgc gtcagattag gatcgttttt cgccttttct gacgatctgc
     2941 gctcggcctc tcaggcttca acctgacaga tcaaccgcta gcgtgcttta ttttccgaat
     3001 tctgagacga cccccataag gtcatccctt aattgtgatc tgaatatcat cctcattcac
     3061 accaaaatat aaagccaacc ctgctttagc ctcttttaga tttatcggcc taactctgtt
     3121 gctatcctcc tcatactcat catattcatc cccgcctttt atcgtattca agactaagga
     3181 atcctcttta ggtaggggtt ggaaatccac tttggagata tcaattccca tgtcttcaag
     3241 ttttctatag gagactggat tacgattgcc gtcccactgc ccagggatat caatttcagc
     3301 ataggaatca atatttatga tccagcgctt agggttttca ggggctcgac ttactccgga
     3361 tattttcccg atcataaatg catgatgatg cttagcctcc tgactacccc agtctttttt
     3421 cgaattttgg acgcaaacca ggtacggaag cttagctacg tgagtagcat caagtctcca
     3481 agcctggctt cctccactat ttaaaatctc agtaataccc ctggccgtaa gaacagtaat
     3541 gcagtcagta tcaagcatag atatctccat ttttgtttta aaaaaggcaa gtcaccgcgt
     3601 aatgacaatc tttttagcat caaaaacgga agagctcaac ataaaaccac aagatatata
     3661 caaataacta tccgaagtac tacgacgatt actcctaggg ctgcaaaaaa accagtaaaa
     3721 cactactcga cttcggccgg agggcttttt ctctctggac tcagtctgag tgacaacatg
     3781 atgtgtgaag taagaacccg ccacaccgcc gcatttaaca taagagttct tggccgcacc
     3841 ccctgagagc ccctcagcgc gtttctgcgg cctgagggcc gtttccggca cctttccccg
     3901 gcccaaaccc tgaaaaacgc ccctgataat ttctgcgcgg ctgcatggct atgcacaaga
     3961 gtgaatggcg gccggtttcg tgaaggaaat accgtgaaag ctccgaaaga aacgcttaat
     4021 tgcacagcag cacatgaacg tgatcagatg aaatctgtct actgccgcac ttcttcacga
     4081 aaggcccgct gagcgtcaga ttcgggcctt tcttactaaa aagatttcaa tagaattttt
     4141 tagcaggcga tttttatcta taaccataca ttgagcatag aagacccaaa tttatgtatg
     4201 actcaataga aaaaaacaaa tcacagccat ctaaacactg agacagcaag ccaaacaaaa
     4261 atcaaagaaa taactccatt tttaatcaaa taatatagtt ttcgattcct tttttttagc
     4321 ttctgacctg catcacgaat agcattaaca tatttaaata aatgatttat agcaccagtc
     4381 ctgtcattat tatcgttggg tgatgctgcc gctgacatta gcaccccacc tatccaccta
     4441 tttatttttt gtgaccaccg ccacttcatc ggcctttcta tatcacagaa caaaataata
     4501 cgtgtctgct cggtatcatt ctgagcccag tgaacaaaag tttcgtcaaa aattatcact
     4561 tcaccatcat gccaactgcg gcgtacacca tcaacttcaa taaaacaccg ttcatcattt
     4621 ggtgttgata atcctaaatg ataacgaact gaaccggcat aagggtcacg atgctttcct
     4681 aaatggcttc ctgaaggcaa ttcagcaaac atcgctgctt taatacaggg aatagagcta
     4741 accagttggg ttgttactgg gcaaagccgc tccgctgatg gatgagcatc tccataccac
     4801 ttgaggtaaa agcgcttcca accgcgctta aaaaatgtat taaatcctgc atcattattt
     4861 ttttcagatg atttaatgtg cccttgcaaa ttaacagctt cgtctcttat aacctgccag
     4921 ttatcaatta atggttgaag ttcaggaaat gtttctgggg gaaaccatgc ctgctttgac
     4981 ggcagcttcg aaaagcctgt cataaacata tttatcggag ccataaatgt tgaatgatca
     5041 aataactgcc tggaaaactt ttgttttatt ttaccgcgag aatgcgcata caatacactg
     5101 ataagaaaaa caaaaataat aataagtgac agcatgtgta tattccttgt tcacgccaat
     5161 aataaaaatc tcttaaagat aacataatga atgcacttaa attataatct aaatgctatt
     5221 tatcaatagt ataatcgaag cttcaccaga acatatctac tacacaggca gagaggattt
     5281 cagcaggaat tatgctgaaa tgaatcaaag ggacggaccg tgatataccc gctgtcgctg
     5341 tgatcgttcg agctccagcg ctttctgatg ccgcgcctcc cgcacctgct gcagctcgac
     5401 ggcattcagc cgctcgacaa ccggcgcagc tgatcgctca aactctgcac ctgcctgctc
     5461 aagcccgtga cgcgctcgct cagcgccgcg ttctcccgtt gcataagacc agacatcgtc
     5521 cgccattccg cgaaggcgct ctcccactcg tccagccttt tcgagtagtc ctgctgtagc
     5581 tgctctaatg cgctcagcaa ctgtttttcc agctctgtca tgtgctattt ccccgccagt
     5641 atcatccagc tcgaatcctc tccgaaccgt cccgcttccg ggttcaccct cacgcagcgg
     5701 cgtctctgct cgccgcaggt cgagagctgc acgccgctgt tccgctcgga cagcatcgcc
     5761 tgcgtgctct tctgctcccg gatgctcacg taattgtcga gtatctgctg actctgccac
     5821 cacagaatgg ccgcgttcga ggcgatcagc agcacggaga ccaggacgat ggtcagccac
     5881 gtctggctga ccatgcgcag catccctttc gtacgctggc tcatggctga ggacagcttc
     5941 cggtcgtggt cgaggatggc ggcgctgatt ctcttctcgc tttcgctcag ttccgctctg
     6001 acagacttct cgtgctcgct gaatgcggct ttcagcatct cgccggtact ctgctgctgc
     6061 gctttcgatt tctgctctaa gtccttcgcc agcgttaaaa gactgttcat agatggctcc
     6121 cttcagtcgg atgtttcgcc ccccgtccgg gtcggcaatg ctgatgctgc tttttgtggt
     6181 gcgtaccacc tcaaaacctg cgctttccag cgcctcagtg acgtcctggc gggttttcag
     6241 ctccccggac gaggccaagg ccagtaaacc ccgtgtaatt gcctgagcgg cttcctgctt
     6301 cgtttccggc agcgcggacg gcgtgaccag cgcccgccgg ttttcaggcg cgttcgggtc
     6361 atgcagcccc agcctgccgt tcacgatggt ctgccaggca tcaatgcgcg gacggtcggc
     6421 gcggtcgtaa tacggctgga gacgcttgcc agtcagcagc tccgtgttcg ggatcaggaa
     6481 gttcag
//
