LOCUS       NZ_CP047739             4863 bp    DNA     circular CON 04-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-5 plasmid pEcl5-3, complete
            sequence.
ACCESSION   NZ_CP047739
VERSION     NZ_CP047739.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747511
            Assembly: GCF_015910265.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910265.1-RS_2024_04_04
            Annotation Date                   :: 04/04/2024 05:37:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,074
            CDSs (total)                      :: 4,959
            Genes (coding)                    :: 4,865
            CDSs (with protein)               :: 4,865
            Genes (RNA)                       :: 115
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 94
            CDSs (without protein)            :: 94
            Pseudo Genes (ambiguous residues) :: 0 of 94
            Pseudo Genes (frameshifted)       :: 35 of 94
            Pseudo Genes (incomplete)         :: 59 of 94
            Pseudo Genes (internal stop)      :: 14 of 94
            Pseudo Genes (multiple problems)  :: 12 of 94
            Pseudo Genes (short protein)      :: 1 of 94
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047739.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-5"
                     /isolation_source="urine"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl5-3"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2012-06-20"
                     /collected_by="Public Health Ontario"
     gene            complement(719..1141)
                     /locus_tag="GVI66_RS25225"
                     /old_locus_tag="GVI66_25195"
     CDS             complement(719..1141)
                     /locus_tag="GVI66_RS25225"
                     /old_locus_tag="GVI66_25195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1141..1332)
                     /locus_tag="GVI66_RS25230"
                     /old_locus_tag="GVI66_25200"
     CDS             complement(1141..1332)
                     /locus_tag="GVI66_RS25230"
                     /old_locus_tag="GVI66_25200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_045265536.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1748..1861
                     /locus_tag="GVI66_RS25235"
                     /old_locus_tag="GVI66_25205"
     CDS             1748..1861
                     /locus_tag="GVI66_RS25235"
                     /old_locus_tag="GVI66_25205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(2098..2466)
                     /locus_tag="GVI66_RS25240"
                     /old_locus_tag="GVI66_25210"
     CDS             complement(2098..2466)
                     /locus_tag="GVI66_RS25240"
                     /old_locus_tag="GVI66_25210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045626380.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     gene            complement(2539..3837)
                     /locus_tag="GVI66_RS25245"
                     /old_locus_tag="GVI66_25215"
     CDS             complement(2539..3837)
                     /locus_tag="GVI66_RS25245"
                     /old_locus_tag="GVI66_25215"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_097414638.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGIIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            4532..4798
                     /locus_tag="GVI66_RS25250"
                     /old_locus_tag="GVI66_25220"
     CDS             4532..4798
                     /locus_tag="GVI66_RS25250"
                     /old_locus_tag="GVI66_25220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013647566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_072050235.1"
                     /translation="MSVYCVSYDLNQAGQNYNALYDELKKSPGWCHPLDSTWLISTGET
                     AQQLSDRLRRHLDNNDTLLVIGVTKEYAGWLTQDTWDWMRTDI"
                     /name="hypothetical protein"
     Typing          1454..1861
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 tgtacgcacc gctaaaacgg cctcagggcg tttctgacgg gaaaccggta aaacaggcaa
       61 caaaccaccc gaaaacccgc cagaaacgcg caggctctgt ttttacggca tgcatgagta
      121 tgcacgcaag tgcatagcgc gaaaattcac gccattcagc cagggtaagt cctgtttccg
      181 gcgttcttcc catccgaaaa cggtgaacgg ggacgcaatt tattgcgtgg ccgtgaagcg
      241 ttgccggcgc agccggaaaa ccggttttca gcacgttcag aaatcgggtt cgtcgacggc
      301 cggcaatcac gttatggcaa gtcgccggac gactcaccac acgattcacc ggcgaatctt
      361 cactagagtc gtacccagag tcgccgagtc taaaaaacaa ccaaacgggg agtcgtgtct
      421 agagtcgggt cgttattaac ttattgtttt tatagtcctg agtcgttacc gactctatga
      481 cgactcgccg gggactctat gacgactcgt catagagtcg gatctatata aataaaattt
      541 aaaaaacgaa atttcaggaa agtaacccac tgatattaaa tacaaatctg caaaatgtta
      601 attttgcgtg gggtgtgggt atttttagtg gtgaggagcg ttcagagatc tgacgatttt
      661 tgggaaggtt caggaactcc ccgggatggc cggagaggaa aacggaagca tgacggggtc
      721 aggatttttt ccagaatttc caccacgggg aatggggtgg tgaagaaggt tcgtgcttgt
      781 gctccagaag ctgcatagcc tgacgaaggg aatcaatatg ctcatctcga gccttaacag
      841 cccctttaag accatcgttt tcagcttcaa gtaaagcgat tctatgttgt aaatcatgta
      901 aaccaacgct gttaacacct gtagattctg cgtcactttt tacatcatat tttacagtat
      961 cgatgacacc atcaagagaa ccaaacactc taataagctc tgaagtatca ataactttaa
     1021 tagccttacc gtgctttaca acagaggtgt aacttaactt cccactgtta atcatcttgt
     1081 aaattgtggg gcgactaacc ttaacaagcc gtgccgcttc actaatagac actctggcca
     1141 tcagttacca tcctgtaatt catccagtct cgaggacagt gtacggaaaa gatgctccgc
     1201 atcatcgtgg agtttctcac aaaggtcggc ctcgacatcg agagcaagct cgttaagctt
     1261 ctccagcagg agcagagact gattctggat gaatttagcc atgttcagcg ctgttttctg
     1321 ttgtttcgtc ataagaagcc tcagagcgtc agtgctcagt gtaaaaccag tctcttttcc
     1381 gcttcctcgc tcactcagtc gctacgctcc ggtcgttcgg ctgcggcgag cggcaagacc
     1441 attcttataa aagctcgaga aaaccgagcg atcaaggtag gaggaaaaga ggaggccgcg
     1501 cagcggccgt ttttccatag gctccgcccc cctgacaagc atcacagaaa cggacgctca
     1561 aatcagtggt ggcgaaaccc gacaggacta taaagatacc tatcatttct gataggcggc
     1621 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgccattcc gctgttatgg
     1681 ccgcgtttat ctcattccac gccagacact cagttccggg taggcagttc gctccaagct
     1741 ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gccttatccg gtaactatcg
     1801 tcttgagtcc aacccggaaa gacatgcaaa agcgccactg gcagcagcca ctggtaactg
     1861 atttagagga gttagtcttg aagttatgcg ccggttaagg ctaaactgaa agaacaagtt
     1921 ttggttactg cgctcctcca agccagttac cttggttcta agagcgccaa ctcttggaac
     1981 cttcgaaaaa accgcccgcc aaggcggttt tttcgtttac agagcgagag attacgacga
     2041 gaacacagtg atctcaagaa gatcttgcct caacatacag tacaatcgta actaagccta
     2101 tttcctgctc ttccaaaacc gatgatcctt atcaaaaaaa actatataaa acacattatc
     2161 ctttaaaaaa ccagcaaccc ttcttttctg gcctcctata ttttttataa caccccagtt
     2221 ctcatcaact gatatttcat cagggcattt aaaatcatta actgctgaat ctggaaatcg
     2281 agtgtagtta gtaaaaatac catcagagct gacttttgtt atcccattag ctgtaagata
     2341 aacaagcttt tcaattaaat ctggcaaact gccgtcaacc ctccaagaat caatagactg
     2401 accaatatca tcgacgttat gaaaatatct aaaactaaca gaaataaatc tatctgtagt
     2461 tttcataatg ctctttgagg attttgtttc ctctttcctc gaagagctac gaagaccgcg
     2521 gcctctcttt gacataaatc atccctcagg cataagtttt tcaaattcat ctaaattatc
     2581 aaacaatggg atagcatcaa atgcaccaga taaataatca tgcccatacg atgattttaa
     2641 agttcgctgc ttctctttat actcaaccaa agaatagaga tgactacttc ctgattttat
     2701 atctttatca acaaaccaaa tctggtcacg tctaaaacaa tcagtgttca ttagattaac
     2761 atcctgaaca gcagctatta tttggctaag cccctcgctt tgcgcatgga atattctaaa
     2821 caaaaactta gacaaaaggg tgtgaaactt agaatcaaac tcatcaacta acaataagtt
     2881 accgtttatc atggcattgt agattggacc aagaagataa actatctttt tagtaccctc
     2941 agactcaaaa tcaataggta ctgaaatctc accgctagct gtttttttca ctactctaag
     3001 agaaaaaacc ttctggtttt ttgcaaaatt ttgtattgaa ctaaataaat tttttaaatt
     3061 atcatcatct gtttttatat ttccaaatgg aaaatcaggt gcgacctcat cgaggtaaat
     3121 atcattaatc tggaatgctg gaagtatttt ttttagccaa gccttaaaat cctcagactc
     3181 atttattaac ttcatggtaa ttgctgtaaa catcccttca ttagaaccag aaaggaatac
     3241 gatattgtta aaccaattaa ctactcgttt tgaatgctca ccattaaaac cagcaacaac
     3301 cgaaacaaat ggaacatcct cccttgtttt ttgaataatc ccttctttga taaaaaggtt
     3361 tgactcacta aaagccgcct tatttacctc aatagaaaga ccatctcttt caaagagaat
     3421 ggtttctctt gattttggtg tgtagtacaa ccattcttct tttattttcc cctgaaaaat
     3481 agacaatcca tatctatatt taaccccatc tgaaataaaa gtcatttcca tttcagatgg
     3541 tttatttggt ccatcctcat caagcaaaaa cggtattacg ttctttgtaa tgctactttc
     3601 aatagcttgc acggaatgca aaacaatatc tttcattaaa gaaattgttt tcatgagatt
     3661 agacttcccg ctagcgttag ccccaaatat cagagcagat ttcagtatcc gaacatcact
     3721 cactgaaacc acgttagcca agtttatgtc gtctttcgcg gatgacaccg ccaacatgct
     3781 catattttga gcatccataa aggaaaatgc gttagaaaga ctaaaattaa ccatcataag
     3841 aacacccctt acgccatcct taaacaacaa aatgagtaaa tattgacaaa atcaggactt
     3901 aaggatagca tacaagcgtt ctaaaacaac aggtgcgttt aatagatagc acgttatgga
     3961 acacttgtta aaaaaagcta aatgcagggg gtctatgaga ttttttctat accaaatggt
     4021 tcctttagcc gcagttgtca gctcagcagt gctagcttta cactcggttt caggatgggg
     4081 atggtttctc tttgtagcat ttatgctcag cggagctgat acagtgaaga aacatctagg
     4141 aaatgacgca tgaaaggaga aaaacgacgt tttttgaagt aagtgggtta gcctgtacca
     4201 ccagaactaa cccgtaacaa accatacaca aatgcggcac ttgagtacca cctcaagagc
     4261 ttgataaagc cagccacaag atttgttggt tgctacagta caattttgat agcaaaaatc
     4321 aacactcatg tggcgaaaaa taggaaatgc accatgcaga cagacgagtc tcaggcatac
     4381 atttttcgcc cttatatcac agtaaagggc aaacggatca cccgtccaaa cggtgggatg
     4441 ttcaaaattc caattaatcg tgaaaagaag aagtaagttg tgactgcagc cctaacgggc
     4501 tgccccacat ttgattaaag aggatggttc tatgtcagtt tactgtgtct cttatgacct
     4561 gaatcaagca ggtcaaaact acaatgcact atatgatgag ttaaaaaaat cgccggggtg
     4621 gtgccaccct ttagattcca catggctaat atcaaccgga gaaacagcgc aacaactgtc
     4681 ggatcgtttg cgtaggcatc tggacaataa tgacaccttg cttgtgattg gtgtaaccaa
     4741 agaatacgca ggatggctca cccaagacac atgggattgg atgagaacag atatatagat
     4801 ataaagccct gcggggcttt ttttacgtct gtagctggtg tggatttaac ataaccccct
     4861 aat
//
