LOCUS       NZ_CP047735             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-6 plasmid pEcl6-6, complete
            sequence.
ACCESSION   NZ_CP047735
VERSION     NZ_CP047735.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747512
            Assembly: GCF_015910245.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910245.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:14:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,909
            CDSs (total)                      :: 4,795
            Genes (coding)                    :: 4,669
            CDSs (with protein)               :: 4,669
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 34 of 126
            Pseudo Genes (incomplete)         :: 89 of 126
            Pseudo Genes (internal stop)      :: 22 of 126
            Pseudo Genes (multiple problems)  :: 17 of 126
            Pseudo Genes (short protein)      :: 1 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047735.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-6"
                     /isolation_source="Rectal"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl6-6"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2014-02-25"
                     /collected_by="Public Health Ontario"
     gene            941..1168
                     /locus_tag="GVI67_RS24395"
                     /old_locus_tag="GVI67_24345"
     CDS             941..1168
                     /locus_tag="GVI67_RS24395"
                     /old_locus_tag="GVI67_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1534..1719
                     /locus_tag="GVI67_RS24400"
                     /old_locus_tag="GVI67_24350"
     CDS             1534..1719
                     /locus_tag="GVI67_RS24400"
                     /old_locus_tag="GVI67_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2010..2228)
                     /locus_tag="GVI67_RS24405"
                     /old_locus_tag="GVI67_24355"
     CDS             complement(2010..2228)
                     /locus_tag="GVI67_RS24405"
                     /old_locus_tag="GVI67_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(1767..1854)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2..526
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.512"
                     /coverage="87.0"
                     /evalue="1.14e-179"
ORIGIN
        1 attccgcttc ctcgctcact gactcgctcc gctcggtcgt tcggctgcgg cgagcgggac
       61 gggcttacgg atggatcaga aatttcctgg gggatgccag gaagatactt aacgaagaaa
      121 gcataaggcc gcgccaaagc cgtttttcca taggctccgc ccccctgaca agcatcacag
      181 aatctgacgc tcaaatcagt ggtggcgaaa cccgatgcgg cttaaagata ccaggcgttt
      241 ccccctggtg gctccctcgt gctcccccgc attcctgcct ttcggtttac cggtgtcact
      301 ccgctgttat ggccgcggtt tctcattcca cgcctgacac tcagttccgg gaaggcagtt
      361 cgctccagga tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc
      421 ggtaactatc aacttgagtc caacccggaa agacacgact aatcgccatt ggcaacggcc
      481 attggcaaac aggatgtgcg agagatgtct caagagttct tgaagtggcg gccttagtga
      541 ggctacactg aaaggacaaa tttggtgaca tgtctcgcac aagactgtta ccacggttag
      601 gggatctgcc aggattacct aaccttcgaa aaaccacctg ccagggtggt tttttcgttt
      661 acagggcaag agattacgcg cggaaaaaaa ggatctcaag aagatccttt ataaattaga
      721 catccaacct cacaaagagg aatacttaaa tcatcttggt atatgtatcg gccggattac
      781 atctcatata agcctcctcc ccgcctgaca ccacactttg accatagcac ctcgcgccta
      841 actcgatata cagcccacca tcaagcctct tttcacataa aaaatgacct atagttagat
      901 ggttagtgac caaaactgat atggataaaa ggagctggct ttgaaaaaga tttttgtttc
      961 agtgttcgca gcggcagttg cgctttcagc cctcaccggc tgcacgcgca caagctatgc
     1021 catacacacc aatgacggtc gcactattgt cagcgacggc aaacctaaag aatcagactc
     1081 aggcctgctt ggctatacag acgcgaatgg tgtcaaacag cagatcaata aaaccgacgt
     1141 taaagaagtc tccgaaattc cgcactgagc atcttctccg aaaaaggccg ctaatgtggc
     1201 ctttttcttt acctgtggtt ctccgccaaa atcccagcaa attgcatcac caaagctaaa
     1261 agctttcagg gttgtctaaa aagcacaaga cataagagga agtgcggtat tttataatca
     1321 agcccccaag aatttaccat aacatccgtt gcccgcaccg cctgagacgc gttcagcgcg
     1381 ttcctgacga aaccatgaca aaaaccacaa caaaccaccc cggaacccgt cagaaacgcg
     1441 cctgttaaat tttaacggca tgcatgacta tgcaccagaa tgacgccatg ctcttttcac
     1501 gcaaaaatca tcaccagacg gggaaaatca ccagtgacca gacaggaatc cgccgccctc
     1561 aatatggcca aatttatccg cgcacagaca cttctcctcc ttgagcggct cgagcagatg
     1621 gatctggatg aggctgccgg ctgctgtgag cacctgcacg atcaggccga agcgctttac
     1681 gccatgctga acgcacagat aggcgaggaa aatgcgtgaa aatcggtgaa cgggtgcgca
     1741 attcagtgcg cggccgtgag gcgatggcgg ggtgtcgggg cgcagccctg accagggtat
     1801 ttgtgatgcc ggcgcgtgcg cggtattaca aatgcacatc ctgtcccgga acggacaccg
     1861 ggaaacagca aaaaaaaccg ggcggcacgc ccggaactca atcaagttag attagattac
     1921 tctcactcgt ccataacagc atcatggaac gacgaccacc gtccgtgacg gccgcctcgt
     1981 ttaagtatgg acagaaatac agaaaatgct caggacgaaa tgtaatgaat gcgaacggat
     2041 tcaagaaatt cgagcatgac agtccttacg gccggttcgg tttcagacaa aatctgccgg
     2101 tatgcatcca gcatcatggc tccggcatcc cctccggcac gccgtagcca gaccgaaaca
     2161 acggacacaa gcaggtgtcg ctcatcatca ctaagagtca tcagggctcc ggaagaaaaa
     2221 ccaaacatgg cttacctact gttaacggca acgcagaaga aaggtttaca gggtagcggg
     2281 cagggatgaa aaaaaaaaaa ttgcagtcag gccggaacgg ctgctgaacc ggtacaggcg
     2341 ggttccgggc gcagcgccgg accagtcacg gagcactagc ggagtgtata ctggcttaag
     2401 atgtagcatg gcagtaacac atagcattaa tgccacataa gacaggcgca gaaagtgaaa
     2461 gaaaacgcgt caggagaaaa tgtgatacag aatat
//
