LOCUS       NZ_CP047734             4781 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-6 plasmid pEcl6-5, complete
            sequence.
ACCESSION   NZ_CP047734
VERSION     NZ_CP047734.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747512
            Assembly: GCF_015910245.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910245.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:14:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,909
            CDSs (total)                      :: 4,795
            Genes (coding)                    :: 4,669
            CDSs (with protein)               :: 4,669
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 126
            CDSs (without protein)            :: 126
            Pseudo Genes (ambiguous residues) :: 0 of 126
            Pseudo Genes (frameshifted)       :: 34 of 126
            Pseudo Genes (incomplete)         :: 89 of 126
            Pseudo Genes (internal stop)      :: 22 of 126
            Pseudo Genes (multiple problems)  :: 17 of 126
            Pseudo Genes (short protein)      :: 1 of 126
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047734.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-6"
                     /isolation_source="Rectal"
                     /db_xref="taxon:158836"
                     /plasmid="pEcl6-5"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2014-02-25"
                     /collected_by="Public Health Ontario"
     gene            77..433
                     /locus_tag="GVI67_RS24360"
                     /old_locus_tag="GVI67_24310"
     CDS             77..433
                     /locus_tag="GVI67_RS24360"
                     /old_locus_tag="GVI67_24310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(<753..875)
                     /locus_tag="GVI67_RS24705"
                     /pseudo=""
     CDS             complement(<753..875)
                     /locus_tag="GVI67_RS24705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1524..1847
                     /locus_tag="GVI67_RS24365"
                     /old_locus_tag="GVI67_24315"
     CDS             1524..1847
                     /locus_tag="GVI67_RS24365"
                     /old_locus_tag="GVI67_24315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_126289757.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2056..>2490
                     /locus_tag="GVI67_RS24635"
                     /pseudo=""
     CDS             2056..>2490
                     /locus_tag="GVI67_RS24635"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2525..3016
                     /locus_tag="GVI67_RS24375"
                     /old_locus_tag="GVI67_24325"
     CDS             2525..3016
                     /locus_tag="GVI67_RS24375"
                     /old_locus_tag="GVI67_24325"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3023..3238
                     /locus_tag="GVI67_RS24380"
                     /old_locus_tag="GVI67_24330"
     CDS             3023..3238
                     /locus_tag="GVI67_RS24380"
                     /old_locus_tag="GVI67_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3909..4151)
                     /locus_tag="GVI67_RS24385"
                     /old_locus_tag="GVI67_24335"
     CDS             complement(3909..4151)
                     /locus_tag="GVI67_RS24385"
                     /old_locus_tag="GVI67_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            4338..4655
                     /locus_tag="GVI67_RS24390"
                     /old_locus_tag="GVI67_24340"
     CDS             4338..4655
                     /locus_tag="GVI67_RS24390"
                     /old_locus_tag="GVI67_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          1238..1411
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          complement(763..1271)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.294"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          1525..1841
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="7.01e-62"
     Typing          1838..3330
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.6"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcgctacagc tggtaataga cgaaatactt atcttcctca tctaacagag agaagtaaaa
       61 aatttaggag taaagcatgt cccaagcaac tagaaaacca tcaactgtag gggatattat
      121 tctttatgaa tatctcgaac caagtggttt aaaaatcaat gacttggctg aaatgttgaa
      181 tgtgcataga aactctgcca gtgctttagt taacaataat agaaagctgt ctaccgatat
      241 ggcttttagg ttggctaaag ccttcgatac cactgcaaag ttctggctaa atatacagca
      301 gtctgttgat ttatgggagg ttactagtag ccctcgattg caagaagaaa tagacagagt
      361 gattcccgct catgaaatca tagaaaggaa acacattgtt gatagttctg agaagaaaca
      421 tgctattgca tgattttttt taaaaaaata taagttatcc ctcaaaaaga aggcttctat
      481 aactagaagc cttctttttg agggataaaa acgctcatgt taactttctt cttacgatct
      541 tcttgagatc cgtgctctgt acgcgtaatc tcttgctctg taaacgaaaa aaccgcctgg
      601 ggaggcggtt tttcgaaggt taagtcagtt ggggaactgc ttaacctggt aactggcttc
      661 agcagagcgc agataccaaa tactgtcctt ccagtgtagc cgtagttagg ccaccacttc
      721 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      781 gccagtggcg ttttgtcgtg tctttccggg ttggactcaa gtcgatagtt accggaacag
      841 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga gcgaactgcc
      901 tacccggaac cgagtgtcag gcgtggaatg agacaaacgc ggccataaca gcggaatgac
      961 accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccatcag ggggaaacgc
     1021 ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agattctgtg
     1081 atgctcgtca ggggggcgga gcctatggaa aaccggctct gccgtggcct tatcgcttcc
     1141 ctgttaagtt tcttcctggc atcttccagg aaatttccgc cccgccagta agccggtacc
     1201 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaatatatc
     1261 ctgtatcgca tattctgctg acgcgccggt gccgcctttt ttctcctgtc acatgaagca
     1321 cttcattgat ttccgcatcc gtgccaacat aataagccag tatacactcc gctagcgctt
     1381 cgtgactggt tcagggctgc gccccgaaac ccgccaaaag cctgacgcgc ctgcggcttg
     1441 tccaaccccg cgccgaccgg gaaagaggtt cccgcccgtc ccggcgttat caggagaccg
     1501 gtttgtcaga gaaacgcagc aaaatgctca cgatgtgggt gaccgaagat gagcaccgcc
     1561 gtctgctgga acgctgcgac ggtaaacagc tcgcggcctg gatgcggcag acctgcctgg
     1621 acgaaaagcc tgcgcgcacc ggaaagctcc cctcaatctc gccggcgctg ctgcgccagc
     1681 ttgccggcat gggcaataac ctgaaccaga tagcccgccg tgttaacgcc ggcggcggca
     1741 ccggccatga cagggtacag attgtggcgg cgctgatggc catcgatgcc gggcttgagc
     1801 ggctgcggca cgccgttctt gagaaaggaa cggacgatga tcgttaaatt tcatccacgc
     1861 ggtcgcggcg gtggcgccgg gccggtggac tatcttctgg gtaaagaccg gcagcgtgaa
     1921 ggtgccagcg tcctgcaggg gaagccggaa gaagtccggg agctgattga cgcgtcgcct
     1981 tacgtgaaga aatacacctc cggcgtcctg tcgtttgcgg aggccgatct gcctcccggc
     2041 cagcgggaaa aactgatggc cagcttcgag cgggttctga tgcccggact tgataaagat
     2101 cagtacagca ttctgtgggt ggagcacgag gacaaggggc gtctggagct gaactttctg
     2161 atccccaata ccgagctgct gaccggcagg cggctccagc cgtactacga ccgcgccgac
     2221 cggccgcgta tcgatgcctg gcagaccatc gtgaacggca gactggggct gcacgacccg
     2281 aacgcaccgg aaaaccgccg ggcgctggtc acgccgtccg gactgccgaa aacgaagcag
     2341 gaggccgcag aagccatcac gcgcggactg ctgacccttg cttcgtccgg ggagctgaaa
     2401 acccgtcagg acgtcactga ggcgctggaa cggtcaggtt ttgaggtggt gcgcaccacg
     2461 aaaagcagta tcagcattgc cgacccggac ggggggcgaa acatccgact gaagggagcc
     2521 atctatgagc agtcttttaa cgctggcgaa ggacttagag cagcaatcga aagcgcagaa
     2581 gcagagtacc ggcgagatgc tgaaagccgc attcagcgag cacgagcagt ctgtcagagc
     2641 ggaactgagc gcaagcgcga ggagaatcag cgacgccatc ctcgcacacg agcagagtat
     2701 gagcgaggcg atggagaaaa accggcggag cgtactgcgg actgccgggc ggacatggct
     2761 caccatcctg atggtgtcag cgctgctgat cggcacgagc gggagcatac tgtggtggca
     2821 ggggcagcag ataaccgaca attacacgca tcttcgtcag caggaagata ccctggcgaa
     2881 aatgaccgcc cggacgtggg gagtgcgcta tcaggagagc agcgacggcc ggaggtttct
     2941 tatcctgccg ccggggatgc aaaccgaagc catcccctac gacgggacga catggatccg
     3001 cctgaaacag gagtagcgga aaatgacaga gctggaaaag cagttgctga gcgcattaga
     3061 gcagctacag caggactact cgcaaaggct ggacgaatgg gagagcgcct tcgcggaatg
     3121 gcggagcatg tgtgggctta tgcaacggga gaacgcggtg ctgaacgagc gcgtgtcgga
     3181 cttgagcacg caggtgctga gtttaagcga gcagctgcgc cgcttgtccg ggaactgaat
     3241 gccatcgagc tccggcagag ggagaatgag tatcagaaaa cactggagct ggagcgccag
     3301 ccgcagaaaa cctaccgcgg gccgacactt taaaccatcc aggcagctga caacccgcac
     3361 gaagaggggc tcttacttct cccgctaaat aggttcgtga tcaaaaaggt cacgacttat
     3421 tcaggagcgg gaaatgaagc tgttaaatct tattgaatca ttgcttaact gcctgtatct
     3481 ggcggcggag ctgtgggact ggctggagaa gcatttgtcg gtttttaagg cgttcgcgct
     3541 gattttagtg tgactttacg cgccatgcat ctggctgcat gactatgcat cagtgtaata
     3601 tccagcggct gcgtttctgg tgttttttcg tcatgtctgt cgccactttc tcgcgtctgg
     3661 caccggaaac gcagccaacc ccgtttcagc ggtgcgccca acggatgtta tggtaaatca
     3721 gtatcagctg cagatataaa aaagccgcca ggctggcggc ttgctgttat tttcgtggct
     3781 ttcctgcatc tgagcgtttt cctctgaccc ttccgtcatt gttacggtca cgaggttgag
     3841 ttccgccgcc tggtttagga acgacaacgt gtgggttgag tggtcgacga tctgccattt
     3901 tctatacctc actgagcata aacaaacaat tgccaacgaa tgttcccttg cgcgggggta
     3961 tgttggtggt ggttttgtgg tgggattgtt ttcccgcctg cgatagttac ttctttaccg
     4021 caaccggtac atttataaat cccttcatat tcacatgttt ctcccggctt atattcagtg
     4081 tcaaaaaccg agttaaactt cgtatctgct tcaaaaatct tgcttttgtt tcctgagcga
     4141 acaagagcca tgcaacctcc aaattatcat ttattttgca cacccgcact gtgcatgagt
     4201 gcattctaca gcatagccat tgattgcaca agtgcactgt gctgttgtgc atttgatatg
     4261 tttgctgtac tatatcggag gtaagaaggt gatcgaaaaa attgccaagt tgcggcaaaa
     4321 attgaggtaa aagaaaaatg cagcaagatc atgaacatga tgattgccgc attcagtttc
     4381 gagacgattg gctataccag ttttacaaat atggtaaagc caatcgaaag attccaactg
     4441 cgattagtgc ttcattaggt cgcaagctgg atatgctgaa tgctgctgat aaagttggag
     4501 atttgcggtc tcccccggga aaccgatttg agaatctcaa ccctccattg acagggtatt
     4561 catccatacg ggtgaatgag aagtaccgct tgatatttaa gtggggtgaa aacggagtcg
     4621 aggatttgta tctcgatcca catgattacc gatagataac aggtcgtggc tgttatctta
     4681 taaaacaagt aggtgaggcg ttccctcacc tacattaaaa tgtcattaca aataatgatt
     4741 aataaaaaaa atgacgtgac tatatttaag ataattactc a
//
