LOCUS       NZ_CP047720             2495 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-E2 plasmid pEclE2-5, complete
            sequence.
ACCESSION   NZ_CP047720
VERSION     NZ_CP047720.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747519
            Assembly: GCF_015910205.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910205.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:08:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,878
            CDSs (total)                      :: 4,764
            Genes (coding)                    :: 4,647
            CDSs (with protein)               :: 4,647
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 32 of 117
            Pseudo Genes (incomplete)         :: 81 of 117
            Pseudo Genes (internal stop)      :: 23 of 117
            Pseudo Genes (multiple problems)  :: 17 of 117
            Pseudo Genes (short protein)      :: 1 of 117
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047720.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-E2"
                     /isolation_source="Sewage water"
                     /db_xref="taxon:158836"
                     /plasmid="pEclE2-5"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2015-07-23"
                     /collected_by="Public Health Ontario"
     gene            complement(19..246)
                     /locus_tag="GVI74_RS24260"
                     /old_locus_tag="GVI74_24205"
     CDS             complement(19..246)
                     /locus_tag="GVI74_RS24260"
                     /old_locus_tag="GVI74_24205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1454..1672
                     /locus_tag="GVI74_RS24265"
                     /old_locus_tag="GVI74_24210"
     CDS             1454..1672
                     /locus_tag="GVI74_RS24265"
                     /old_locus_tag="GVI74_24210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(1963..2148)
                     /locus_tag="GVI74_RS24270"
                     /old_locus_tag="GVI74_24215"
     CDS             complement(1963..2148)
                     /locus_tag="GVI74_RS24270"
                     /old_locus_tag="GVI74_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          1829..1916
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(662..1190)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ctttttcgga gaagatgctc agtgcggaat ttcggagact tctttaacgt cggttttatt
       61 gatctgctgt ttgacaccat tcgcgtctgt atagccaagc aggcctgagt ctgattcttt
      121 aggtttgccg tcgctgacaa tagtgcgacc gtcattggtg tgtatggcat agcttgtgcg
      181 cgtgcagccg gtgagggctg aaagcgcaac tgccgctgcg aacactgaaa caaaaatctt
      241 tttcaaagcc agctcctttt atccatatca gttttggtca ctaaccatct aactataggt
      301 cattttttat gtgaaaagag gcttgatggt gggctgtata tcgagttagg cgcgaggtgc
      361 tatggtcaaa gtgtggtgtc aggcggggag gaggcttata tgagatgtaa tccggccgat
      421 acatatacca agatgattta agtattcctc tttgtgaggt tggatgtcta atttataaag
      481 gatcttcttg agatcctttt tttccgcgcg taatctcttg ccctgtaaac gaaaaaacca
      541 ccctggcagg tggtttttcg aaggttaggt aatcctggca gatcccctaa ccgtggtaac
      601 agtcttgtgc gagacatgtc accaaatttg tcctttcagt gtagcctcac taaggccgcc
      661 acttcaagaa ctcttgagac atctctcgca catcctgttt gccaatggcc gttgccaatg
      721 gcgattagtc gtgtctttcc gggttggact caagttgata gttaccggat aaggcgcagc
      781 ggtcggactg aacggggggt tcgtgcatac agtccatcct ggagcgaact gccttcccgg
      841 aactgagtgt caggcgtgga atgagaaacc gcggccataa cagcggagtg acaccggtaa
      901 accgaaaggc aggaatgcgg gggagcacga gggagccacc agggggaaac gcctggtatc
      961 tttaagccgc atcgggtttc gccaccactg atttgagcgt cagattctgt gatgcttgtc
     1021 aggggggcgg agcctatgga aaaacggctt tggcgcggcc ttatgctttc ttcgttaagt
     1081 atcttcctgg catcccccag gaaatttctg atccatccgt aagcccgtcc cgctcgccgc
     1141 agccgaacga ccgagcggag cgagtcagtg agcgaggaag cggaatatat tctgtatcac
     1201 attttctcct gacgcgtttt ctttcacttt ctgcgcctgt cttatgtggc attaatgcta
     1261 tgtgttactg ccatgctaca tcttaagcca gtatacactc cgctagtgct ccgtgactgg
     1321 tccggcgctg cgcccggaac ccgcctgtac cggttcagca gccgttccgg cctgactgca
     1381 attttttttt tttcatccct gcccgctacc ctgtaaacct ttcttctgcg ttgccgttaa
     1441 cagtaggtaa gccatgtttg gtttttcttc cggagccctg atgactctta gtgatgatga
     1501 gcgacacctg cttgtgtccg ttgtttcggt ctggctacgg cgtgccggag gggatgccgg
     1561 agccatgatg ctggatgcat accggcagat tttgtctgaa accgaaccgg ccgtaaggac
     1621 tgtcatgctc gaatttcttg aatccgttcg cattcattac atttcgtcct gagcattttc
     1681 tgtatttctg tccatactta aacgaggcgg ccgtcacgga cggtggtcgt cgttccatga
     1741 tgctgttatg gacgagtgag agtaatctaa tctaacttga ttgagttccg ggcgtgccgc
     1801 ccggtttttt ttgctgtttc ccggtgtccg ttccgggaca ggatgtgcat ttgtaatacc
     1861 gcgcacgcgc cggcatcaca aataccctgg tcagggctgc gccccgacac cccgccatcg
     1921 cctcacggcc gcgcactgaa ttgcgcaccc gttcaccgat tttcacgcat tttcctcgcc
     1981 tatctgtgcg ttcagcatgg cgtaaagcgc ttcggcctga tcgtgcaggt gctcacagca
     2041 gccggcagcc tcatccagat ccatctgctc gagccgctca aggaggagaa gtgtctgtgc
     2101 gcggataaat ttggccatat tgagggcggc ggattcctgt ctggtcactg gtgattttcc
     2161 ccgtctggtg atgatttttg cgtgaaaaga gcatggcgtc attctggtgc atagtcatgc
     2221 atgccgttaa aatttaacag gcgcgtttct gacgggttcc ggggtggttt gttgtggttt
     2281 ttgtcatggt ttcgtcagga acgcgctgaa cgcgtctcag gcggtgcggg caacggatgt
     2341 tatggtaaat tcttgggggc ttgattataa aataccgcac ttcctcttat gtcttgtgct
     2401 ttttagacaa ccctgaaagc ttttagcttt ggtgatgcaa tttgctggga ttttggcgga
     2461 gaaccacagg taaagaaaaa ggccacatta gcggc
//
