LOCUS       NZ_CP047719             4781 bp    DNA     circular CON 15-APR-2024
DEFINITION  Enterobacter hormaechei strain Eho-E2 plasmid pEclE2-4, complete
            sequence.
ACCESSION   NZ_CP047719
VERSION     NZ_CP047719.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747519
            Assembly: GCF_015910205.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910205.1-RS_2024_04_14
            Annotation Date                   :: 04/14/2024 21:08:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,878
            CDSs (total)                      :: 4,764
            Genes (coding)                    :: 4,647
            CDSs (with protein)               :: 4,647
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 117
            CDSs (without protein)            :: 117
            Pseudo Genes (ambiguous residues) :: 0 of 117
            Pseudo Genes (frameshifted)       :: 32 of 117
            Pseudo Genes (incomplete)         :: 81 of 117
            Pseudo Genes (internal stop)      :: 23 of 117
            Pseudo Genes (multiple problems)  :: 17 of 117
            Pseudo Genes (short protein)      :: 1 of 117
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047719.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="Eho-E2"
                     /isolation_source="Sewage water"
                     /db_xref="taxon:158836"
                     /plasmid="pEclE2-4"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2015-07-23"
                     /collected_by="Public Health Ontario"
     gene            complement(join(4595..4781,1..170))
                     /locus_tag="GVI74_RS24225"
                     /old_locus_tag="GVI74_24170"
     CDS             complement(join(4595..4781,1..170))
                     /locus_tag="GVI74_RS24225"
                     /old_locus_tag="GVI74_24170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075993.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="WP_023316024.1"
                     /translation="MSQATRKPSTVGDIILYEYLEPSGLKINDLAEMLNVHRNSASALV
                     NNNRKLSTDMAFRLAKAFDTTAKFWLNIQQSVDLWEVTSSPRLQEEIDRVIPAHEIIER
                     KHIVDSSEKKHAIA"
                     /name="HigA family addiction module antitoxin"
     gene            complement(373..690)
                     /locus_tag="GVI74_RS24230"
                     /old_locus_tag="GVI74_24175"
     CDS             complement(373..690)
                     /locus_tag="GVI74_RS24230"
                     /old_locus_tag="GVI74_24175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023316025.1"
                     /translation="MQQDHEHDDCRIQFRDDWLYQFYKYGKANRKIPTAISASLGRKLD
                     MLNAADKVGDLRSPPGNRFENLNPPLTGYSSIRVNEKYRLIFKWGENGVEDLYLDPHDY
                     R"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            877..1119
                     /locus_tag="GVI74_RS24235"
                     /old_locus_tag="GVI74_24180"
     CDS             877..1119
                     /locus_tag="GVI74_RS24235"
                     /old_locus_tag="GVI74_24180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023316026.1"
                     /translation="MALVRSGNKSKIFEADTKFNSVFDTEYKPGETCEYEGIYKCTGCG
                     KEVTIAGGKTIPPQNHHQHTPAQGNIRWQLFVYAQ"
                     /name="hypothetical protein"
     gene            complement(1790..2005)
                     /locus_tag="GVI74_RS24240"
                     /old_locus_tag="GVI74_24185"
     CDS             complement(1790..2005)
                     /locus_tag="GVI74_RS24240"
                     /old_locus_tag="GVI74_24185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2012..2503)
                     /locus_tag="GVI74_RS24245"
                     /old_locus_tag="GVI74_24190"
     CDS             complement(2012..2503)
                     /locus_tag="GVI74_RS24245"
                     /old_locus_tag="GVI74_24190"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015060400.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2538..3242)
                     /locus_tag="GVI74_RS24555"
                     /old_locus_tag="GVI74_24195"
                     /pseudo=""
     CDS             complement(<2538..3242)
                     /locus_tag="GVI74_RS24555"
                     /old_locus_tag="GVI74_24195"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3181..3504)
                     /locus_tag="GVI74_RS24255"
                     /old_locus_tag="GVI74_24200"
     CDS             complement(3181..3504)
                     /locus_tag="GVI74_RS24255"
                     /old_locus_tag="GVI74_24200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_126289757.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4153..>4275
                     /locus_tag="GVI74_RS24560"
                     /pseudo=""
     CDS             4153..>4275
                     /locus_tag="GVI74_RS24560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(3618..3791)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.429"
                     /coverage="100.0"
                     /evalue="5.6200000000000006e-73"
     Typing          3758..4266
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.294"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(1699..3191)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.6"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3188..3504)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.34"
                     /coverage="99.0"
                     /evalue="7.01e-62"
ORIGIN
        1 aaagccatat cggtagacag ctttctatta ttgttaacta aagcactggc agagtttcta
       61 tgcacattca acatttcagc caagtcattg atttttaaac cacttggttc gagatattca
      121 taaagaataa tatcccctac agttgatggt tttctagttg cttgggacat gctttactcc
      181 taaatttttt acttctctct gttagatgag gaagataagt atttcgtcta ttaccagctg
      241 tagcgatgag taattatctt aaatatagtc acgtcatttt ttttattaat cattatttgt
      301 aatgacattt taatgtaggt gagggaacgc ctcacctact tgttttataa gataacagcc
      361 acgacctgtt atctatcggt aatcatgtgg atcgagatac aaatcctcga ctccgttttc
      421 accccactta aatatcaagc ggtacttctc attcacccgt atggatgaat accctgtcaa
      481 tggagggttg agattctcaa atcggtttcc cgggggagac cgcaaatctc caactttatc
      541 agcagcattc agcatatcca gcttgcgacc taatgaagca ctaatcgcag ttggaatctt
      601 tcgattggct ttaccatatt tgtaaaactg gtatagccaa tcgtctcgaa actgaatgcg
      661 gcaatcatca tgttcatgat cttgctgcat ttttctttta cctcaatttt tgccgcaact
      721 tggcaatttt ttcgatcacc ttcttacctc cgatatagta cagcaaacat atcaaatgca
      781 caacagcaca gtgcacttgt gcaatcaatg gctatgctgt agaatgcact catgcacagt
      841 gcgggtgtgc aaaataaatg ataatttgga ggttgcatgg ctcttgttcg ctcaggaaac
      901 aaaagcaaga tttttgaagc agatacgaag tttaactcgg tttttgacac tgaatataag
      961 ccgggagaaa catgtgaata tgaagggatt tataaatgta ccggttgcgg taaagaagta
     1021 actatcgcag gcgggaaaac aatcccacca caaaaccacc accaacatac ccccgcgcaa
     1081 gggaacattc gttggcaatt gtttgtttat gctcagtgag gtatagaaaa tggcagatcg
     1141 tcgaccactc aacccacacg ttgtcgttcc taaaccaggc ggcggaactc aacctcgtga
     1201 ccgtaacaat gacggaaggg tcagaggaaa acgctcagat gcaggaaagc cacgaaaata
     1261 acagcaagcc gccagcctgg cggctttttt atatctgcag ctgatactga tttaccataa
     1321 catccgttgg gcgcaccgct gaaacggggt tggctgcgtt tccggtgcca gacgcgagaa
     1381 agtggcgaca gacatgacga aaaaacacca gaaacgcagc cgctggatat tacactgatg
     1441 catagtcatg cagccagatg catggcgcgt aaagtcacac taaaatcagc gcgaacgcct
     1501 taaaaaccga caaatgcttc tccagccagt cccacagctc cgccgccaga tacaggcagt
     1561 taagcaatga ttcaataaga tttaacagct tcatttcccg ctcctgaata agtcgtgacc
     1621 tttttgatca cgaacctatt tagcgggaga agtaagagcc cctcttcgtg cgggttgtca
     1681 gctgcctgga tggtttaaag tgtcggcccg cggtaggttt tctgcggctg gcgctccagc
     1741 tccagtgttt tctgatactc attctccctc tgccggagct cgatggcatt cagttcccgg
     1801 acaagcggcg cagctgctcg cttaaactca gcacctgcgt gctcaagtcc gacacgcgct
     1861 cgttcagcac cgcgttctcc cgttgcataa gcccacacat gctccgccat tccgcgaagg
     1921 cgctctccca ttcgtccagc ctttgcgagt agtcctgctg tagctgctct aatgcgctca
     1981 gcaactgctt ttccagctct gtcattttcc gctactcctg tttcaggcgg atccatgtcg
     2041 tcccgtcgta ggggatggct tcggtttgca tccccggcgg caggataaga aacctccggc
     2101 cgtcgctgct ctcctgatag cgcactcccc acgtccgggc ggtcattttc gccagggtat
     2161 cttcctgctg acgaagatgc gtgtaattgt cggttatctg ctgcccctgc caccacagta
     2221 tgctcccgct cgtgccgatc agcagcgctg acaccatcag gatggtgagc catgtccgcc
     2281 cggcagtccg cagtacgctc cgccggtttt tctccatcgc ctcgctcata ctctgctcgt
     2341 gtgcgaggat ggcgtcgctg attctcctcg cgcttgcgct cagttccgct ctgacagact
     2401 gctcgtgctc gctgaatgcg gctttcagca tctcgccggt actctgcttc tgcgctttcg
     2461 attgctgctc taagtccttc gccagcgtta aaagactgct catagatggc tcccttcagt
     2521 cggatgtttc gccccccgtc cgggtcggca atgctgatac tgcttttcgt ggtgcgcacc
     2581 acctcaaaac ctgaccgttc cagcgcctca gtgacgtcct gacgggtttt cagctccccg
     2641 gacgaagcaa gggtcagcag tccgcgcgtg atggcttctg cggcctcctg cttcgttttc
     2701 ggcagtccgg acggcgtgac cagcgcccgg cggttttccg gtgcgttcgg gtcgtgcagc
     2761 cccagtctgc cgttcacgat ggtctgccag gcatcgatac gcggccggtc ggcgcggtcg
     2821 tagtacggct ggagccgcct gccggtcagc agctcggtat tggggatcag aaagttcagc
     2881 tccagacgcc ccttgtcctc gtgctccacc cacagaatgc tgtactgatc tttatcaagt
     2941 ccgggcatca gaacccgctc gaagctggcc atcagttttt cccgctggcc gggaggcaga
     3001 tcggcctccg caaacgacag gacgccggag gtgtatttct tcacgtaagg cgacgcgtca
     3061 atcagctccc ggacttcttc cggcttcccc tgcaggacgc tggcaccttc acgctgccgg
     3121 tctttaccca gaagatagtc caccggcccg gcgccaccgc cgcgaccgcg tggatgaaat
     3181 ttaacgatca tcgtccgttc ctttctcaag aacggcgtgc cgcagccgct caagcccggc
     3241 atcgatggcc atcagcgccg ccacaatctg taccctgtca tggccggtgc cgccgccggc
     3301 gttaacacgg cgggctatct ggttcaggtt attgcccatg ccggcaagct ggcgcagcag
     3361 cgccggcgag attgagggga gctttccggt gcgcgcaggc ttttcgtcca ggcaggtctg
     3421 ccgcatccag gccgcgagct gtttaccgtc gcagcgttcc agcagacggc ggtgctcatc
     3481 ttcggtcacc cacatcgtga gcattttgct gcgtttctct gacaaaccgg tctcctgata
     3541 acgccgggac gggcgggaac ctctttcccg gtcggcgcgg ggttggacaa gccgcaggcg
     3601 cgtcaggctt ttggcgggtt tcggggcgca gccctgaacc agtcacgaag cgctagcgga
     3661 gtgtatactg gcttattatg ttggcacgga tgcggaaatc aatgaagtgc ttcatgtgac
     3721 aggagaaaaa aggcggcacc ggcgcgtcag cagaatatgc gatacaggat atattccgct
     3781 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt accggcttac
     3841 tggcggggcg gaaatttcct ggaagatgcc aggaagaaac ttaacaggga agcgataagg
     3901 ccacggcaga gccggttttc cataggctcc gcccccctga cgagcatcac agaatctgac
     3961 gctcaaatca gtggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4021 atggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt
     4081 tatggccgcg tttgtctcat tccacgcctg acactcggtt ccgggtaggc agttcgctcc
     4141 aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctg ttccggtaac
     4201 tatcgacttg agtccaaccc ggaaagacac gacaaaacgc cactggcagc agccactggt
     4261 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4321 aactacggct acactggaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4381 aggttaagca gttccccaac tgacttaacc ttcgaaaaac cgcctcccca ggcggttttt
     4441 tcgtttacag agcaagagat tacgcgtaca gagcacggat ctcaagaaga tcgtaagaag
     4501 aaagttaaca tgagcgtttt tatccctcaa aaagaaggct tctagttata gaagccttct
     4561 ttttgaggga taacttatat ttttttaaaa aaaatcatgc aatagcatgt ttcttctcag
     4621 aactatcaac aatgtgtttc ctttctatga tttcatgagc gggaatcact ctgtctattt
     4681 cttcttgcaa tcgagggcta ctagtaacct cccataaatc aacagactgc tgtatattta
     4741 gccagaactt tgcagtggta tcgaaggctt tagccaacct a
//
