LOCUS       NZ_CP047706             3991 bp    DNA     circular CON 22-MAY-2024
DEFINITION  Klebsiella pneumoniae strain Kpn-14 plasmid pKpn14-6, complete
            sequence.
ACCESSION   NZ_CP047706
VERSION     NZ_CP047706.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747524
            Assembly: GCF_015910145.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3991)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3991)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910145.1-RS_2024_05_22
            Annotation Date                   :: 05/22/2024 03:28:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,111
            CDSs (total)                      :: 5,988
            Genes (coding)                    :: 5,781
            CDSs (with protein)               :: 5,781
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 82 of 207
            Pseudo Genes (incomplete)         :: 122 of 207
            Pseudo Genes (internal stop)      :: 39 of 207
            Pseudo Genes (multiple problems)  :: 31 of 207
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047706.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3991
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kpn-14"
                     /isolation_source="Rectal"
                     /db_xref="taxon:573"
                     /plasmid="pKpn14-6"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2014-09-29"
                     /collected_by="Public Health Ontario"
     gene            297..569
                     /locus_tag="GVI79_RS30330"
                     /old_locus_tag="GVI79_29935"
     CDS             297..569
                     /locus_tag="GVI79_RS30330"
                     /old_locus_tag="GVI79_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     gene            1084..2124
                     /locus_tag="GVI79_RS30335"
                     /old_locus_tag="GVI79_29940"
     CDS             1084..2124
                     /locus_tag="GVI79_RS30335"
                     /old_locus_tag="GVI79_29940"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040198047.1"
                     /translation="MIELILTYLNKVLLFALRKDSLMAFFNLLFVASLICFGGVMGSYS
                     RGCRDSQNKFSKDKDENNKRASVYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGT
                     KFIEQILLLISVSGISAYLGYALLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDR
                     SKELIEQLEMDKKTTKFELGYFKAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESL
                     SLVKREDVAKDDYDKLLVLKAYILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQY
                     ILRRCQADNSDIKDMIRRALTIKVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEE
                     LKVAIK"
                     /name="hypothetical protein"
     gene            complement(2351..2463)
                     /locus_tag="GVI79_RS30670"
                     /pseudo=""
     CDS             complement(2351..2463)
                     /locus_tag="GVI79_RS30670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2871..3062
                     /locus_tag="GVI79_RS30340"
                     /old_locus_tag="GVI79_29945"
     CDS             2871..3062
                     /locus_tag="GVI79_RS30340"
                     /old_locus_tag="GVI79_29945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040198051.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3062..3484
                     /locus_tag="GVI79_RS30345"
                     /old_locus_tag="GVI79_29950"
     CDS             3062..3484
                     /locus_tag="GVI79_RS30345"
                     /old_locus_tag="GVI79_29950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040214596.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2352..2823)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.777"
                     /coverage="92.0"
                     /evalue="7.8799999999999974e-146"
ORIGIN
        1 cgttcaccgt tttcagtctg gtttggtgcg ggttttgagt tgcctggcct gaattcaggc
       61 tgaaatcccc agacagtcag tcaagtgcat aacgattcat gcccgtaaaa cgccctggag
      121 cgattttgag gcgttttcag ggggtaagac gtaggattta atgggttacg ccccagaatc
      181 gttctgaggc cgttttagcg gtgcgtgtaa tgggacgtta tggtaaatca acggcggatg
      241 cctgagtctg tgttagagtc agcggattgt gataatcagt gactcaggga gatgttttgg
      301 ctactgacgc tcaggtaaaa gaaattaatg cactcatcaa aaaatacccg gactcatgct
      361 ccatctgtca tgaagtctat gatgaggatg atgtgaccta caccgttttt gggtatgacc
      421 ggaaaggtaa aatacaggtc accacgggat gctgtgccgg aatgctgaca gagccggttc
      481 tgctgggtgt ctgtgggtgc tttgacccgg aagaacgtga cgagatcatg cagaatcacc
      541 ccatggcaaa gcagtttttc acagagtgat tccgtatctg ttgagtctga agcccgcctt
      601 gtgcgggctt tttcatatct gcgtgaaatg aggcgttatg gtaaatgcac gcattcattc
      661 ttttgaaaaa tccagaatga cgaatacatg gggcatgttt atatgactat tgagaggaag
      721 gatgttattg taaaaaaatc ctttaaattc aatcatttaa ttatggtagc caaaggggcg
      781 gggcttcgct tattcctaag gaataagcga agccccgccc ctttggctac cagtgcatta
      841 tacttttttt ttcacccaat gtcaatacat gggttgattt tatcatttta agcatttttg
      901 ttgttgtgag tgctgggttt atgccctata ttcagcgcta agcaagcatg ggcttgcaaa
      961 ggaacaatta atgagaggtg ctctatgaaa aaaatcaaac ttcacgatga gcgtccagac
     1021 ttgtaagtgt caagtcagca gaaaaaggca gccaaggctg ccttttttgt tattggttag
     1081 tatatgattg agcttatctt aacttacctg aacaaagttt tattgtttgc tttgcgcaag
     1141 gattctctca tggctttttt taacctattg tttgttgcat ctctaatttg ttttggtggt
     1201 gttatgggga gctattccag aggttgccga gactcccaaa ataaattttc aaaagataag
     1261 gatgagaata ataaaagagc tagtgtttat agatttggta tagcatccgc atttatttgt
     1321 gttcctttca tatcatcatt tcttcatgtt gattactcaa gtataatatt tcctgtggct
     1381 gatggtggtg gaactaagtt catagaacaa atattgcttc ttatttctgt ttcggggatc
     1441 tctgcgtatc ttgggtacgc attactggat ggtcttgcca acaaagtatt aaaagagcaa
     1501 gtagacggta tagataaaaa gcaacaagat ttggaggctg aacaagatga gtttaaagat
     1561 gagcttgata ggagcaagga gctgatagaa cagcttgaaa tggataagaa aacaacaaaa
     1621 ttcgagctag gatactttaa agccatttca gctgtggata aagcagaatc aatgatgtcc
     1681 attccggatg aggctctttc tgttaagaaa aaactaactg aggcattaga tgctgtaacg
     1741 gaatcattgt ctcttgttaa aagagaggat gtagctaagg atgactacga caagctctta
     1801 gtgcttaaag cttatatttt gaaaagactt gatcgtattg atgatgctct gtcaattacg
     1861 gatgagttac ttatgagtaa tgaggataac cctatactta tttacaataa ggcttgctat
     1921 caatatattt tgcgtcgttg ccaagctgat aactccgata ttaaggatat gattcgcagg
     1981 gctctgacta ttaaagttac agatcctgaa ttcattcgca ggcaagaaaa aataagaacc
     2041 aaggtcatag gaaataaaga taatgatctt gaagggttgt ttactgacgc tgagctggag
     2101 gaattaaagg tggctatcaa ataggcagtg gctagctcta tgaattccaa aaagatcctt
     2161 gacgatcctt tgaggtgcag cgtactctac ccctcaaacg aaaaaaccac ccggcaaggt
     2221 ggtttttcga aggttcagta accggcaagt ttctgaaccg aggtaactgg cttagaggag
     2281 cgcagtcacc aaaacagttc gtttagttta gccttaactg gcgcataact tcaagactaa
     2341 ctcctctaaa tcaattacca gtggctgctg ccaatggcga taagtcgtgt ctttccgggt
     2401 tggactcaag agatagttac tggaacgggc gcagcagtcg gactgaacgg ggggttcgtg
     2461 catacagtcc agcttggagc gaactgcctt cccggagccg agtatcaggc atggaatgag
     2521 aaaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     2581 cacgagggag ccaccagggg gaaacgcctg gtatctttaa gtcctgtcgg gtttcgccac
     2641 cactgatttg agcgtccgtt tccgcgatgc ttgtcagggg ggcggagcct atggaaaaac
     2701 agccgttcgg cggcctttgc ttttctgcct catatcttgg ttgcctacgt ttttatgtac
     2761 acgctcctgc cgctcgccgc agccgaacgg ctggagcgta gcgactgagt gagcgaggaa
     2821 gcggaaaaga gactagtttg acattgagca ctgacgctct gaggcttctt atgacaaaac
     2881 aacagaaaac agcgctgaac atggctaaat tcatccagaa tcagtctctg ctcctgctgg
     2941 ataagcttaa cgagcttgat ctcgatgtcg aggccgacct ttgtgagaaa ctccatgatg
     3001 atgcggagca tcttttccgt acactgtcct cgagactgga tgaattacag gatggtaact
     3061 gatggccata gtgtctatta gtgaagcggc acggcttgtt aaggttagtc gccccacaat
     3121 ttacaagatg attaacagtg ggaagttaag ttacacctct gtggtaaagc acgggaaagc
     3181 tattaaagtt attgatactt cagagctaat tagagtgttt ggttctcttg atggtgtaat
     3241 cgatactgta aaatatgatg taaaaagtga cgcagaatct acaggtatta acagcgttgg
     3301 tttacatgat ttacaacata gaatcgcttt acttgaagct gaaaacgatg gtcttaaagg
     3361 ggctgttaag gctcgagatg agcatattga ttcccttcgt caggctatgc agcttctgga
     3421 gcacaagcac gaaccttctt caccacccca ttccccgtgg tggaaattct ggaaaaaatc
     3481 ctgatcccgc catgcttcca ttttgctctc cggccattcc ggggagttcc tgaaccttcc
     3541 caaaaatcgt cagatccctg aacgctcctc accactaaaa atacccacac actacgcaaa
     3601 attaaaattt tgcagataga cctttatttt caatcagtta tctattttaa attacgcaat
     3661 tttatttttt gtaaggtgaa tccgagtcgt ggacgactct atggcgaatc gccgatgact
     3721 tgcccacgat tcatatgtga atcacgccat ataatcagta cgttatgatg acttgactct
     3781 agagtcgact tgccgggttg gtggcttttt gaccatggcg actctagagt caagtcacga
     3841 gtcgaatcgc cggtgaatcg tgtggtgagt cgtccggcga cttgccataa tgtgattgct
     3901 ggtctcgaca aacccgattc ctgaacgtgc tgaaaaccgg ttttccggct gcgccggcaa
     3961 cgcttcacgg ccacgcaata aattgcgcac c
//
