LOCUS       NZ_CP047705             9730 bp    DNA     circular CON 22-MAY-2024
DEFINITION  Klebsiella pneumoniae strain Kpn-14 plasmid pKpn14-5, complete
            sequence.
ACCESSION   NZ_CP047705
VERSION     NZ_CP047705.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13747524
            Assembly: GCF_015910145.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Kohler,P., Tijet,N., Kim,H.C., Johnstone,J., Edge,T., Patel,S.N.,
            Seah,C., Willey,B., Coleman,B., Green,K., Armstrong,I., Katz,K.,
            Muller,M.P., Powis,J., Poutanen,S.M., Richardson,D., Sarabia,A.,
            Simor,A., McGeer,A. and Melano,R.G.
  CONSRTM   Toronto Invasive Bacterial Diseases Network (TIBDN)
  TITLE     Dissemination of Verona Integron-encoded Metallo-beta-lactamase
            among clinical and environmental Enterobacteriaceae isolates in
            Ontario, Canada
  JOURNAL   Sci Rep 10 (1), 18580 (2020)
   PUBMED   33122675
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Melano,R.G., Tijet,N. and Seah,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Research, Public Health Ontario, 661
            University Avenue, Toronto, Ontario M5G 1M1, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.8-BETA
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Illumina MiSeq, Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_015910145.1-RS_2024_05_22
            Annotation Date                   :: 05/22/2024 03:28:21
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,111
            CDSs (total)                      :: 5,988
            Genes (coding)                    :: 5,781
            CDSs (with protein)               :: 5,781
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 82 of 207
            Pseudo Genes (incomplete)         :: 122 of 207
            Pseudo Genes (internal stop)      :: 39 of 207
            Pseudo Genes (multiple problems)  :: 31 of 207
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047705.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kpn-14"
                     /isolation_source="Rectal"
                     /db_xref="taxon:573"
                     /plasmid="pKpn14-5"
                     /geo_loc_name="Canada:Ontario"
                     /lat_lon="43.6532 N 79.3832 W"
                     /collection_date="2014-09-29"
                     /collected_by="Public Health Ontario"
     gene            join(8525..9730,1..984)
                     /locus_tag="GVI79_RS30285"
                     /old_locus_tag="GVI79_29890"
     CDS             join(8525..9730,1..984)
                     /locus_tag="GVI79_RS30285"
                     /old_locus_tag="GVI79_29890"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            2570..4531
                     /locus_tag="GVI79_RS30290"
                     /old_locus_tag="GVI79_29895"
     CDS             2570..4531
                     /locus_tag="GVI79_RS30290"
                     /old_locus_tag="GVI79_29895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4531..5262
                     /gene="mobC"
                     /locus_tag="GVI79_RS30295"
                     /old_locus_tag="GVI79_29900"
     CDS             4531..5262
                     /gene="mobC"
                     /locus_tag="GVI79_RS30295"
                     /old_locus_tag="GVI79_29900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5269..5799
                     /locus_tag="GVI79_RS30300"
                     /old_locus_tag="GVI79_29905"
     CDS             5269..5799
                     /locus_tag="GVI79_RS30300"
                     /old_locus_tag="GVI79_29905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5827..6006)
                     /locus_tag="GVI79_RS30305"
                     /old_locus_tag="GVI79_29910"
     CDS             complement(5827..6006)
                     /locus_tag="GVI79_RS30305"
                     /old_locus_tag="GVI79_29910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6075..6452
                     /locus_tag="GVI79_RS30310"
                     /old_locus_tag="GVI79_29915"
     CDS             6075..6452
                     /locus_tag="GVI79_RS30310"
                     /old_locus_tag="GVI79_29915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(6514..6813)
                     /locus_tag="GVI79_RS30315"
                     /old_locus_tag="GVI79_29920"
     CDS             complement(6514..6813)
                     /locus_tag="GVI79_RS30315"
                     /old_locus_tag="GVI79_29920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6803..7066)
                     /locus_tag="GVI79_RS30320"
                     /old_locus_tag="GVI79_29925"
     CDS             complement(6803..7066)
                     /locus_tag="GVI79_RS30320"
                     /old_locus_tag="GVI79_29925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            7443..8528
                     /locus_tag="GVI79_RS30325"
                     /old_locus_tag="GVI79_29930"
     CDS             7443..8528
                     /locus_tag="GVI79_RS30325"
                     /old_locus_tag="GVI79_29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     Typing          complement(1190..1770)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4532..5259
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 aatatatttg atcatggttc acattcttcg aaaaaaccca tgaagatgtc aggtgatgca
       61 tcctacgacg tcactgaaca aaaaagcgcc tacgcagcaa ctcattttct acttagtgat
      121 aactggaaaa tattgctggg tggccgtctt gactggtatg aatatacaag taaaggtaca
      181 tccattaata caggttataa agttaccaga aatatcacca aatatgcagg ttcaacatac
      241 aatcttgacg atcatcatac catttatggg agctataccg atatattcaa tccacagtca
      301 agcaccgacc tcaatggtaa agtgatcaag cctattgttg gtaagaatta tgagacaggt
      361 attaagggtg agtattttaa cggcttgctt aatgcctctc tggcggtatt tcaggtagac
      421 caggaaaatc gagccaggct gcttcccgat cagacagggt gtgttactaa tggcaatcct
      481 caatgctatg aggctagcgg taaagtacgc acaaaaggga tagatttcga acttcagggc
      541 atggttacgc cactctggga aattggggtg ggatatacat atgcagatac gcgatatata
      601 aaagatggag atcgtagtaa tgaaggcaag cgattcaata ctgccgtgtc gccacaaaat
      661 ttatttaaga tttcgagcat gtacaccttt gacggaaaat taacaaactg gcgagcagga
      721 gtaaacacgt actggcaaag taaaatatat gctaacataa cagacacaag ggatggcaat
      781 ccggttatta ataaacaaaa ggcttattca acaactgaca ttattattgg ctatactcca
      841 atgccacaac tggatttaca gctcaatata actaatgtct ttgataaggt ttattataca
      901 tccataggtg aatcgattgg atatggcacg gaaacatatg gagaaccaag aaatgttaaa
      961 attagtgcaa cctatagatt ctaaccatgc ggattgggtc ttctcctcgg atttgcccct
     1021 ataaatccgt cttttgtggt tccggcgtac aaccccctgt ttgccacttc agttgatggt
     1081 taatcaaatc atgtagagga tcttcttgag atcctccatg gttcgtcgta atcttttgcc
     1141 ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga aggttaagtc agttggggaa
     1201 ctgcttaacc gtggtaactg gctttcgcag agcacagcaa ccaaatctgt ccttccagtg
     1261 tagccggact ttggcgcaca cttcaagagc aaccgcgtgt ttagctaaac aaatcctctg
     1321 cgaactccca gttaccaatg gctgctgcca gtggcgtttt tgcgtgtctt tccgggttgg
     1381 actcaagtga acagttaccg gataaggcgc agcagtcggg ctgaacgggg agttcttgct
     1441 tacagcccag cttggagcga acgacctaca ccgagccgag ataccagtgt gtgagctatg
     1501 agaaagcgcc acacttcccg taagggagaa aggcggaaca ggtatccggt aaacggcagg
     1561 gtcggaacag gagagcgcaa gagggagcga cccgccggaa acggtgggga tctttaagtc
     1621 ctgtcgggtt tcgcccgtac tgtcagattc atggttgagc ctcacggctc ccacagatgc
     1681 accgaaaaag cgtctgttta tgtgaattct ggcaggaggg cgaagcctat ggaaaaacgc
     1741 caccggcgcg gccctgccgt tttgcctcac atgttagtcc cctgcttatc cacggaatct
     1801 gtgggtaact ttgcgagagt tagcagcgcc cgccgcagtc tcacggccgg agcgtagcga
     1861 ctgagtgagg gaggaggcgc aatatccccc ctcccgcaga cggccaccgg acgcgcggat
     1921 gtgacatatt gcaccgtgtt accgcctttc ccttccctgc cgataaatca ttcagatagg
     1981 tgaagtctgt cggttttctg ctgaatattt tacgttaacc tatctctgtt gcttttttct
     2041 ccttgcggag aaatgcaggt catccccgct ggggcgtggc tgacttcagg aggaaatccc
     2101 ctgcttacgc tggctggacg ccaccgacgc acggtctttc cccctctttc tggctcagct
     2161 cggctggacg ccggccacgc cagaaattca cccggccccc gtcaggctca cgcctgacca
     2221 cccacaaaaa cccacggtgc tgaacgcacc tgacgaagca gcaaagctgc tcctgatttt
     2281 gcgggtggtc agccgctcgc tatggcgaac tctgacgggg gccggaacac cggaaaagac
     2341 cgcactgggg tgccgccttt tcctctcaca cgctggggcg tgctcgttgt tcctccgggg
     2401 ctgcgccttt tccgcttggg gcggaaagag gctggcctcc gtggtgaaag ctgaatgcaa
     2461 tcaccacgga cgtgaccgcc gccgccatgc ctcggggcat gtccgcggct tgtgcagcag
     2521 gggctgcaca ccacgccggt aaacccgtcg tctgaaggag agagattgca tgtttaatac
     2581 ggattcgctt gcctggcagt ggcatatccc ggagctggcc cggcagattt acccgtgggt
     2641 cagctggctg tacagggagt attacggtct ggccggggct ttcctggtta tcggcctgtt
     2701 tttcggtctg gctgaactct gggccggacg gatgcgtttt gtggggctga gtcataagga
     2761 gcgacgggag tatgccgatc gctataagcg cgataataac cggactgcgc cattttcggt
     2821 gctggcttcg ctggattcgc ccgggcggat ggtcagggca cggcggcatg tctggaacta
     2881 cgcgtactgg ctgaccggcg ctgccggtct ggcccttctg ggggggctgg ttgtcgccgt
     2941 gtttatcgag agctggggag aaaccgtcct gcgtccggac ctctacgcga acgcagatta
     3001 ttacctgctg cagccggcta acctgttcgg gattgtctgg tgtgccggag ggcttgtcgc
     3061 cggcatcctt ctggcgcggc tgctggcatt tctgctgctt gacggtcatt ttgcggccgc
     3121 tgacgaggcg gtgaatgcga aactgaacca ggagagccgg cgcagcagcc agcggaccgg
     3181 agaaatgacc gacgtccgcc acctgcattt cggggagccg gtaccggtta acgcgctggc
     3241 cgatttttcc acagagcagg cccggaaaca gcaggcggtg tttctgggta aggatgagca
     3301 gggccagccg gtgctggtcc cgcgtgatac ctggcgcaag accaatattc agatcctcgg
     3361 cctgcccggg agcggcaaga gcgtgatggg caccaacgcg ctcattcgct gcgtacgcga
     3421 tttcggggac gcggtggtgt acttcgaccc caaaggggac gcgtgggcgc cgcacgtctt
     3481 ccgggcgcac tgcccggact tcaccctgct ggatctgcgg ccggggaaac cggcgcagct
     3541 gaatctgttt cgcgatctcg accagtacgc cctgaagaat ctgctggttg ccgggtttaa
     3601 cctgagtgag accggcgacg tggcggacca ctaccgcatc agcgagcaga aagcggcgaa
     3661 gctgattgcg gagcagttcc cgcagggggc aaacattcag caggtgctgg cggcggcata
     3721 cgcgctgccg gaggccctga aaaaggacgt gaaggggctg atcaccaagc tggagaacgt
     3781 ggccgacctg agcgtcctgc agacggacag cggcatcgac gtggcgggga taatcaacgg
     3841 cggcggctgc ctgtacgtca tcggctcgat ggacgatgag gcggtgatcc gggtgcagaa
     3901 gatgctgttt gcccgctgcg cgcaaatcat cattgcgcgg gatgagttcc ggaggtggcc
     3961 gcacgcgagc atcatgctcg atgagattaa atacctgctg tcgaagtacg tgctgaacgc
     4021 gctggggacg ctgcgcagtc gggactgcaa cctgctgctc gcgcaccagt cgctgggcga
     4081 cttcgggcag tgcggccagg atctgcccgc tgagtttgtg aaaaccacgg tgctggataa
     4141 cacgccgatc cgctggttct accgggcggc cagccaggag tccgcgcagt gggcggcggg
     4201 ccagaccggg gaaatccggg tggacgtgga gcgccgccgg gccagccggg aggcggggaa
     4261 cgtggagcat atcagcgggg acagcttcat tcagaaggag gcgcggccgc tgtttgacgt
     4321 gaacaccctg cagcacctgc ctgacgggtt tgcggtgatg accgggctgg gcgtggcccg
     4381 gatggcgttc agcagcccgc tgcgggttga acgccgggaa atcccgctgc agcgcttccc
     4441 ggtgctggcg aaaacggacc cgctggcgga gtaccagccg gaggccgggc ggcagcggcc
     4501 gggtgatgat gattttgcgg ggctgtactg atggcactgg agcgatacaa cgtcagccac
     4561 gcgaagcggc aggcgcggaa cgcggagaaa acccggctga ccctgcgctg gctgcgggaa
     4621 gagctgtgtt ccacggcgga gctggttgcg cgacggctgg ggattgcggc ggtgcagccg
     4681 gtttaccgct tcctggacag cctggtggcg aaagggctgc tggtccgggc gaaatacccg
     4741 gttgacggcc gtcaggtcag cgtgtgggga ctgacgccgc acggggtggc gttctcgttc
     4801 gacgaggacg aaccgctgac ggacgttatt ccgtttcagc cgtcaagggt gtcggcggcg
     4861 cagctgccgc accggctggc cgtgcagtcc ctgcgtctcg ccatggaggc ccggggggcc
     4921 tccggctggc ggtacctgca ccgtatggcc ctgaagggga tgaaggtgcc ggacgcgctg
     4981 gcggagctgg acggcagaac ggtggcgttt gaggtggagc gcaccgtgaa gagccgcagg
     5041 cgctatcagg aggtggtgtc cgggtatctg ttcaatcgca aagcgaacgg catcgatgaa
     5101 atctggtaca tctgcccgga ccgggcgacg caggtcaggg tgcagcgggc gatcctgtcg
     5161 gtggatgaaa tcgtgaatcc acagaccggc gaggcccgca aaacggcgga gctggaccgg
     5221 gagcggctgt ttgcgtgctt taaatttatg acaacggagt gagggattat ggcgctgtac
     5281 atggtattta ttgaagacgc ggaggaccgg gacggcgagc gctgcatcat gccgaccagc
     5341 gagcatcttg aagatatggt ggtgctcgcc tggcagaccg gggcgtatgt tttctgtttt
     5401 gtcgaccggg cccagcatct ggcgatgatg aaatccatcg ggatgtatga cgagcggctc
     5461 aacagcgacg aaaaagccac gtactttttc accggtaaaa atccgcaatg ggaacgggac
     5521 aaacccgcag gaatgaagct gtgtgattac ctctatcgca aggtggagcg gttcgccatc
     5581 gagaacggtg tgcaggtggt tgatgccggg aagtgctcac cggagcagat tagcgcgatg
     5641 attgaggtgg tcagcgagcg gcacggggaa agcgcggtga acgagaagct gaaagcggag
     5701 ctgatgcggg aatataacag ccgctatttt aacgtgccgg tgcctgaact gccggaggac
     5761 aaacccgccc gccggtggtg gccgttcggc aaaaaataaa cggcagcacc ggccatgccg
     5821 gtgctgttat tccttcccga gcttatcccg catcacctgc cagagtgttt ccgcatcctc
     5881 gtgcagccgc tcgcacatgt cggccggggt atccatatcc agctcgttca gtttctcgag
     5941 taacagcagc gactggtcct taataaattt agccatattg agggcagttt tttcctgctt
     6001 agtcatgttt tctgtccttc cgggtgtggt gtgcttcctg tcgggtatac tgtctcaaaa
     6061 ccggaggtga gacaatggcg tgggtgacgg tgagacaggc gacggaactg acggggaaag
     6121 cccggagttc cctgtatagg gatatggcga aaggccgcgt atcataccgt actgaagctg
     6181 atggtggtcg ggtggtggat accagcgaac ttatccgggt gtacggtgaa ttaagacaga
     6241 ttgagactca cgagcgggac gggttgagac tgtcggatga gactgaaaaa atggcctccg
     6301 acgcgttgat cgcggagata aaagcactca gggaggaggt ggccggactg cggcaggaaa
     6361 tgcaggccat gagactgctg gaacacaaac cagagacagc agagacaccg gccggaacat
     6421 cgcgccgatg gtggcagtgg ggaaaacgct gacgcgctgt gcttgtccaa ccggcgacgg
     6481 ccacgcaatg aattgcgccc ccatcaccgg ttatcagcga acggtatggg gattctggcg
     6541 ctggccaacc acggccagaa cccggacttt cctggtctgg tcatcaacat catagaggac
     6601 atgctgtccc atcagactga tccggcgaac gtcctcgcca tagcgtgagt ccacttcata
     6661 taggcgcggg aagtcggcca gtttgtccgc tgcagcgaga actcgatcgg taacgttctg
     6721 cgcatcccac agggatgaat tttccgcaat gtagtcaaac aatgctgtca catcctcgat
     6781 ggctccctgc ttccattcca cctcatacgg catcgcgtag ttcctgatag cggccatgca
     6841 gtctctggcg gagttgttcc gtggtcatgc ccgggcgcgt gtcagcgcgg gcggcggtca
     6901 ctttagcgcg aacctgatct tcatattctg cctgggccag ttcttcgtta gccaggcgct
     6961 gcaacagtgc agctttgtcc gggccacctt caggcagtat gcggggcagt ctctcggcca
     7021 gttcccgcag gaaattggcg ttttcatagt gtgtactgtt gctcattgtg gagcctcccg
     7081 ggctgatacg tcgatatgaa ggagtttagc agggggcggt ctgaaagaat atgctcgtgt
     7141 cagtcaggct gatgaagacg aaagctggta aaaaagggct tacgacaccc attttacgtc
     7201 aaactgcacg acatgcagtt gcgtgcatag ccatgcagcg ggttaaaatt tatcaggcgc
     7261 gtttccggcg gttttccggg tggtttgttg ccggttttat cccgtagccg ccggaaatgc
     7321 cctcagaccg tctgagcggt gcgcgtaatg cgacgttatg gtaaatcatg tgattaatgg
     7381 tagtgataat cattatcact tttgctatat tcttgtttct tttattgtaa ggaaatgtaa
     7441 gtatggcaat cacgattaaa tccgccgtta atgtccgtag cagagtatta atatactctt
     7501 tactactgtt tactggttca gcgtatgcgg ctgatatagc tgaatcaaca ttcctgactc
     7561 gtgatatcgg caatggtctg tatgaaatgt cttttgatgg caaaaataat agtctctggg
     7621 cagcatcatc gtcttcattc gacagagata agggccacgg aattgtttat cagttagaac
     7681 catccactct gaaaacaaaa tctgccgtta aaatcgcacg tcttgggttt gccacagcac
     7741 tggatgagga acatgagcga ctttatatcg gcaatactct ggacggctca gtgacggtca
     7801 ttgatactct aaccggaaaa gaactttcag taattccgtt aactgaggga caggatgagg
     7861 ctcatttcag accgaccaga gaaatggtca ttgacaaaaa aaatcagcgt ctttatgtct
     7921 ccagcgccgc agataaaggg gctctgtggg ttattgacat ggctgtcggg aagaaaatta
     7981 aaaccattga tggactgggg cacgctgtta ctggaattgc gttaaataaa aatggggata
     8041 atatttatct ggttaacggg gatggtgaga ttattaacct ggatgccagt agctatgaaa
     8101 taaaaaaacg gtttgttgta gagcctgaga aaaaacactt tttccttaat gtaagtctgg
     8161 atgaaaaaaa cggacgtgca tttattaccg acccggatat tcccggggtg ctggttgttg
     8221 atgtaaactc gggaaaaata ctacacagaa ttgatgttat aaattcactg gcgattttat
     8281 ttaatccttt acggaacgaa atctatatca cgcaccgtaa tgcaaaacag gtcagcattg
     8341 tggacagtaa ttcttattac gtaaaaacaa cgataaaaac ccgcctgatg ccaaacagtc
     8401 tgtctttatc acatgacggt gaaaatctgt acgttagtgt aaaacaagaa aaaaaagaga
     8461 tgagtactat gaaggattac atactcaaaa tcgaattatc taaaattgct atggagccag
     8521 aagaatgaat aaatatttag ggctgagcat agtatatatg atgtatacat catctgttat
     8581 ggctgatgat tctggcgcag ttagtaaaga taaaaatatt tcaccagaag aaacattaat
     8641 tgttcatggg accgctgttt ctgaaagcac aacagaagga acagattcat acacaaccca
     8701 gtcagccaat acagcatcaa agatgaatct gtcattccgg gatacacctc agtcaactac
     8761 agtcattacg cgacgaagaa tggatgatgt agcagcaaaa aatattattg atgtggttaa
     8821 aaccgctcct ggattatatg tcagtacagc agaaggttcc ggaagaccga attttatgtc
     8881 cagaggattt aatgctaatg tcatgtatga aggatttagc ctgccgtgga attcatacat
     8941 tccaagttca caaactagtt cagctatttt tgaccgggtg gagatagtcc gcggggcaac
     9001 aggtcttgtt cagggtgccg gagacccttc agccgcgata aatatgatac acaagcgtcc
     9061 gacccggcaa tatcaggcca gcactaacat cggtgccgga cggtggaatg actaccgggg
     9121 tggggttgat gtatcaggac cactgaatga cagcgcgacc ttacgcggaa gggcagttgt
     9181 ggatggcgaa aacagtgaga ccttcaggga tagtgagaaa cgcaggaata acgtcattta
     9241 cggaattatg gaggcagatc tgactgataa taccctactt acgattgggg gatatcagca
     9301 gtatgaatat aacaatcatt ggtggtatga cttacctatt tctggtaccg gacaccacct
     9361 taatcttcct cgctcgacgt ttacaggcaa tgactgggag tttgaaagaa gtaaaagtaa
     9421 aactgcattt gcaactctgg agcagaagtt cagtaatagc tggaagctga aattttcaac
     9481 gctgcaaaac tggagaaatg ctgacctgtt agggacggca acataccggc ataaagatac
     9541 ccgacaaagt atgtatcagc aagtatgggg atcactgagg gaatataatt ccaaaagcta
     9601 tgacctgtcg cttaatgggg actatgcttt atttggtcgt gttcatcaat tcgtggctgg
     9661 aacaactcag caaaaataca ccgataaaga tacaggttat acatattccc caatatatat
     9721 tgagaatgtt
//
