LOCUS       NZ_CP047661             6938 bp    DNA     circular CON 24-JUN-2024
DEFINITION  Escherichia coli strain LD39-1 plasmid pLD39-1-6kb, complete
            sequence.
ACCESSION   NZ_CP047661
VERSION     NZ_CP047661.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13829544
            Assembly: GCF_009909465.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Li,R. and Wang,Z.
  TITLE     Prevalence of plasmid Mediated Colistin Resistance Gene mcr-1 in E.
            coli from Deer in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Lu,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-2020) Colloege of Veterinary Medicine, Yangzhou
            University, Shuangqiao Street, YangZhou, JiangSu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: DEC-2019
            Assembly Method                   :: Unicycler v. V0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009909465.1-RS_2024_06_24
            Annotation Date                   :: 06/24/2024 03:43:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,003
            CDSs (total)                      :: 4,883
            Genes (coding)                    :: 4,646
            CDSs (with protein)               :: 4,646
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 237
            CDSs (without protein)            :: 237
            Pseudo Genes (ambiguous residues) :: 0 of 237
            Pseudo Genes (frameshifted)       :: 93 of 237
            Pseudo Genes (incomplete)         :: 142 of 237
            Pseudo Genes (internal stop)      :: 54 of 237
            Pseudo Genes (multiple problems)  :: 46 of 237
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047661.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LD39-1"
                     /isolation_source="feces"
                     /db_xref="taxon:562"
                     /plasmid="pLD39-1-6kb"
                     /geo_loc_name="China: Dafeng"
                     /lat_lon="32.56 N 120.42 E"
                     /collection_date="2018-08"
                     /collected_by="Xiaoyu Lu"
     gene            join(6889..6938,1..1189)
                     /locus_tag="GUU82_RS24580"
                     /old_locus_tag="GUU82_24585"
     CDS             join(6889..6938,1..1189)
                     /locus_tag="GUU82_RS24580"
                     /old_locus_tag="GUU82_24585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015975693.1"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid replication protein, II/X family"
                     /protein_id="WP_201763283.1"
                     /translation="MIDMLVLRIPFKPYLVNERLDSAGNYVAHVDLTEVARRSGLILSA
                     HSVEYAIDGDLTVSGLKHRYESLASHYTGVAFKLFEGGLNCEPCVELKASPAKILQGHN
                     VFGPTDFELCSLEFFGILSESMPDLYELLDIPNTSVSRIDVTFSARVQTQAMANQVINY
                     LRNVSNGQTKKTKAQDYDTTVTWNGGSRHRTLVSYLKWNEMQAQITRLRRKKSCQLSQY
                     EKNALHVLSNPDLQEFAVGLVRFEARLHTRYFESFGLPRNLINFVKYQNSYPTGKFECV
                     CDLWKKAFKDIFGALEGAEMNIYDDSKVYDSLCDAFSTVTKTGNISKSKANRLFGFYRR
                     LVNEGYDNVAMTMDRMTFWRHEKDLTSVGLSKAQLKNLTAEKNNVVPFIRAINVDFMNQ
                     YPAWYQEPMSRYA"
                     /name="phage/plasmid replication protein, II/X family"
     gene            1589..1861
                     /locus_tag="GUU82_RS24585"
                     /old_locus_tag="GUU82_24590"
     CDS             1589..1861
                     /locus_tag="GUU82_RS24585"
                     /old_locus_tag="GUU82_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590119.1"
                     /GO_function="GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="WP_112924724.1"
                     /translation="MVKVEIKPSQVSVDVRNGVSAKTGKPYTMREQMGYAWLGGDYPEL
                     IKLSLDEGQPPYTAGFYELLLSSFRVGDFNRLQVDRVRLSPIDGK"
                     /name="single-stranded DNA-binding protein"
     gene            1861..2058
                     /locus_tag="GUU82_RS24590"
                     /old_locus_tag="GUU82_24595"
     CDS             1861..2058
                     /locus_tag="GUU82_RS24590"
                     /old_locus_tag="GUU82_24595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_160183298.1"
                     /translation="MDISGTLCDAAQICQQVSLTLTGEAPKITDYSYLGKVWGLAFSSV
                     MSLYLFSLGIGTVLRFIKNV"
                     /name="hypothetical protein"
     gene            2067..2300
                     /locus_tag="GUU82_RS24595"
                     /old_locus_tag="GUU82_24600"
     CDS             2067..2300
                     /locus_tag="GUU82_RS24595"
                     /old_locus_tag="GUU82_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003033737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="WP_160183299.1"
                     /translation="MSILKKVIVGAFAVAAAVSAPAVMAAEGTTEVDLTPLTSAISFSG
                     VTTAILGIAAAVAGLYALYAGVKHVLRMVRSA"
                     /name="major capsid protein"
     gene            2387..3742
                     /locus_tag="GUU82_RS24600"
                     /old_locus_tag="GUU82_24605"
     CDS             2387..3742
                     /locus_tag="GUU82_RS24600"
                     /old_locus_tag="GUU82_24605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_160183300.1"
                     /translation="MRMGRYTRFKRVVVYFLCMMIAYGSFPRIAFANPVIIGLGEIFGS
                     VIARRAAIYSAEKAADIAFTRQVAAATIQNVEKSALAKQLLVPKSNFLSRAGQATWAGL
                     GTVGTAYSLYDLFDDVFDSSVKFVNGEANSSGGYTVTVDGVQTDVPFSPVPVYRSPANA
                     GIGEMKLWNGQLVYMKDTSGKDVRGMPYLPAYRNPTGSTWVIYSLTDEPIFNTWINTTG
                     TKGSTTYWYSPNGQTGATQVPIFTYQRTLKAGETLPNIGSSILQSADELQQVAGQTEVK
                     AEVLAQTINDLMHDAATQTGYDGVPFSDTDAVTVAEIKDLLAKENGIVTKAGVFDPAFK
                     SDGTVNIPNKVKTSESPGIDLGENPNIKEPDLGDTPTAKQIMEPVITPLSWIDNMKLSG
                     HAAVCPTGSFEVFDTLITIDSQCDLFERLRDIAKVVSTLIWAIVALRVVLSA"
                     /name="hypothetical protein"
     gene            3750..4037
                     /locus_tag="GUU82_RS24605"
                     /old_locus_tag="GUU82_24610"
     CDS             3750..4037
                     /locus_tag="GUU82_RS24605"
                     /old_locus_tag="GUU82_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 family protein"
                     /protein_id="WP_160183301.1"
                     /translation="MFGIVLSALNSVLAYLLRGVVLKFVLFTALFYAVKELFSVVADRL
                     PQSGQVAELFNRLPDGMWYFIYYFQADIGIPLVFSALFTRFIIRRLPIIG"
                     /name="DUF2523 family protein"
     gene            4040..5083
                     /locus_tag="GUU82_RS24610"
                     /old_locus_tag="GUU82_24615"
     CDS             4040..5083
                     /locus_tag="GUU82_RS24610"
                     /old_locus_tag="GUU82_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="WP_160183302.1"
                     /translation="MAISAYVGVPGHGKSYEVVGSVILPAYLAGRRIVTNIDGVKESNF
                     YEYAKRHNKDLNSLGTIINCTNDDVLSPQFLPYKDAEEETFCKSGDLIAIDEAWRFWDS
                     DKNICKEHKAFFAEHRHMSDKDTLATCDIVVINQSLDTIARFIKTRIETTYRMQKHVSV
                     GLKNRYRVDVFTGVKLFKNSRVTSYQNKYNKEIFALYHSHYGGQGDEQTTDDRQNIFKQ
                     KKLWVLFIFIIIMLCSSGYFVFSFFKGSTPENKEVSTPQNKQLTDKVTVQNNEVVPKQP
                     VIQKPGLSKRWRINGTLVKEGKSFVILTDSVGLNRLVSRAGFSNDGLMLNGVVDGEIVS
                     YFSGEKS"
                     /name="zonular occludens toxin domain-containing protein"
     gene            5080..6294
                     /locus_tag="GUU82_RS24615"
                     /old_locus_tag="GUU82_24620"
     CDS             5080..6294
                     /locus_tag="GUU82_RS24615"
                     /old_locus_tag="GUU82_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="WP_160183303.1"
                     /translation="MKKLLFFILVVFSLPSFAVKVDFRLDAMQLPKAINFFYSDVIKQP
                     FMLAPELANDTRVVSFHVTEDVDAKAFFVRYLSNMNVAVSNKNGVDYIYMVTPKQVDVP
                     KYTFVYTPKHRSVAYLSSLLRGIVGGDFGSVAGAEPVQGVTMQPSVARTGNINGDGDVF
                     VYYGTRSDIRKIEEVLPVIDTPSSEVYVGGYVFEVQTNERNGSGLQLAAKLLNDKLSIQ
                     IGGTAEGGGQNFVKFSSGSLNALYELFRTDSRFHVVSSPSLRARSGVQASFSVGEDVPV
                     LGNVSYEGDKAVQSVEYRSSGVIFNVLPYVRSDVIDLNISQELSNFAKTDTGVNNSPTL
                     IKRSISTAVSVKDGDIILIGGLAENKTNDTDTGLTFMPSWFSSKSSEKYKTDIVVVLQA
                     RKVAR"
                     /name="type II secretion system protein GspD"
     gene            complement(6603..6791)
                     /locus_tag="GUU82_RS24620"
                     /old_locus_tag="GUU82_24625"
     CDS             complement(6603..6791)
                     /locus_tag="GUU82_RS24620"
                     /old_locus_tag="GUU82_24625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_160183304.1"
                     /translation="MSGTSKRTTVTINRHMAELLKNATVEATTRANLPIKQSDLVNYLI
                     KHKLEEAIEGVLKEKGL"
                     /name="hypothetical protein"
     ARG             complement(6604..6791)
                     /name="FONA-6"
                     /gene="FONA-6"
                     /gene_name="FONA beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="100.0"
                     /coverage="21.02"
ORIGIN
        1 tgagaggttg gattctgctg gtaactatgt agctcacgtt gacctaacag aggtcgcccg
       61 ccgttctggg cttattcttt ccgctcattc cgttgaatac gcaatcgacg gtgatttgac
      121 ggtttcaggc ctgaaacatc gttatgaatc attagcttct cactatacag gcgttgcctt
      181 caagcttttt gaaggtggtc tgaactgtga gccatgcgta gagcttaagg cgtccccggc
      241 gaaaattctg caaggccata acgttttcgg acctacagat tttgaacttt gctctctcga
      301 gttcttcggg atcctttctg agtctatgcc tgatctctac gaactgcttg atatcccaaa
      361 cacttctgtt tcccgaatag acgtaacttt tagtgctcgg gtacagactc aggcaatggc
      421 taatcaggta attaactacc ttcgtaatgt aagtaatggt cagactaaaa aaactaaggc
      481 gcaggactac gacacaacag ttacctggaa cggcggttct agacatcgta ctcttgtatc
      541 atatttgaaa tggaatgaga tgcaggcgca gataacgaga ttgcgccgta aaaaatcctg
      601 ccagctcagt cagtatgaaa aaaatgctct gcatgtcctg tctaatccag atctacagga
      661 atttgctgtt ggattagtac gctttgaagc tcgtttacat acccgttatt ttgaatcttt
      721 tggattgcct cgtaatttaa ttaactttgt taagtatcaa aattcttatc cgaccggaaa
      781 gtttgaatgt gtttgtgatt tgtggaagaa agcttttaaa gatatcttcg gcgcgttaga
      841 gggtgcagaa atgaatattt atgatgattc taaagtttac gattctttgt gtgatgcgtt
      901 ctcaacagta accaaaaccg ggaatatttc aaaatctaag gcaaatcgtt tgtttggttt
      961 ttaccgtcgc ctcgtaaatg aaggttatga caatgttgcg atgacaatgg atcgtatgac
     1021 tttttggcgt catgaaaagg atctaacttc tgttggcctt tctaaagctc agctcaagaa
     1081 tctgacggca gaaaaaaaca acgttgtgcc ttttattcgt gcgatcaatg tggatttcat
     1141 gaaccagtat cctgcatggt atcaggagcc gatgtcacga tatgcttaaa gaaaaaagta
     1201 acttttctaa tactgaggag tgcccgttaa ctcctcagtt aattttgtta atattgaaaa
     1261 actattatgt ttgcgatgcc tgcaatggtg agaaattgat atgtgaaaaa tgtgataatc
     1321 acggaatatt aatgcacgat gaattaatat tcgattctct ttttagagaa taattatacc
     1381 gggttaatta aacctctatt atatagaggc ggtattctat tatctttatt tcggtaaaaa
     1441 taaatttgta aataggataa taaaatgttt gcaattgtgt tcggttggat aatatcattc
     1501 attcagatta tttttgagtc ctgtctttat actttgataa tcgcaatcgt acttacgtta
     1561 atccctcgct tattatttgg agttagaaat ggttaaagtt gaaattaaac cttctcaggt
     1621 ttctgtagat gttcgtaatg gtgtttcggc taaaaccggc aagccttata caatgcgtga
     1681 gcaaatgggc tatgcctggt tgggtggcga ttacccggaa ttaatcaagc tttctcttga
     1741 tgagggtcag ccaccatata cagctggttt ttatgagctt ctgctttcca gtttccgggt
     1801 tggtgacttt aaccgtctcc aggttgatcg cgttcgtctt agtccaatcg atggtaaata
     1861 atggatatat ctggaacgtt gtgtgacgct gcacaaatat gccagcaagt atctttaacg
     1921 ttaactggcg aagcaccaaa aattactgat tattcatatt taggtaaagt ttggggttta
     1981 gctttttcct ctgtaatgtc gctttatctt ttctctctgg gaataggtac ggtattacgt
     2041 ttcattaaaa atgtataagg tgaattatgt ctatcctgaa aaaagtaatt gttggtgcgt
     2101 tcgctgttgc tgctgctgtt tctgctcctg ctgtaatggc tgctgaaggt accaccgaag
     2161 ttgatctgac gcctctgact agtgctatta gcttctctgg tgttactact gcgattctgg
     2221 gtattgcagc cgctgtggct ggtctgtatg ccctttacgc tggcgttaag catgtgctgc
     2281 gtatggttcg ttccgcttaa ttaaatctcg ggaggggagg ggagaccctc cctttttcta
     2341 tggaattaca gactatagat tttgtggttt tcctctgggg gttgatgtgc gcatgggccg
     2401 ttatacaagg tttaaaaggg tagttgttta ttttctctgc atgatgattg cttacggcag
     2461 tttccccaga attgcgtttg caaacccggt aattattggt cttggtgaaa tcttcggatc
     2521 cgttattgct cgccgtgctg ctatatattc cgccgaaaag gcagctgata ttgcatttac
     2581 caggcaagta gcggctgcaa ccattcagaa cgtcgaaaaa tcagctttag cgaaacagct
     2641 tttagttccc aaaagtaact ttttatctcg agccggtcag gcaacctggg cgggtcttgg
     2701 tacggtcgga actgcatatt ctctttacga tctcttcgat gacgtgttcg actcaagtgt
     2761 taaattcgta aatggagaag ctaactctag tggcggttac actgtcactg tagacggcgt
     2821 acagacagat gtgccgtttt cgccagtgcc tgtataccgt agccctgcta atgcgggtat
     2881 aggtgaaatg aagctttgga acggtcagct tgtttatatg aaggatactt caggcaagga
     2941 tgtgcgtggt atgccatatc tcccggcgta tcgcaatcca actggttcta cgtgggtaat
     3001 atactcgttg accgacgaac caatttttaa tacctggatt aatacgacag gaactaaggg
     3061 atcaacaact tactggtatt cgccaaacgg tcaaactggt gcaactcagg taccaatttt
     3121 cacatatcag cgaactttaa aagcaggtga gactttacct aatattggat cctcgatcct
     3181 tcaatctgct gacgaactgc aacaggtagc tggtcagacc gaagtaaaag cggaagttct
     3241 cgcacaaact atcaatgatt tgatgcacga cgcagcaacg caaacgggtt atgacggcgt
     3301 gccttttagt gatactgatg ctgttactgt agctgagatt aaagatttac tggctaagga
     3361 aaatggaatt gttacaaaag caggtgtttt tgatccggca tttaagtctg atggcactgt
     3421 aaacattcca aataaggtta agacgtctga atctcctggc attgatttgg gagagaatcc
     3481 aaatattaaa gagccagatt tgggcgatac tccgaccgca aaacagatta tggaaccggt
     3541 aataacaccg ctttcatgga tagataatat gaagctttca ggacatgcag ctgtttgccc
     3601 tactggtagc tttgaggtgt tcgatactct cattactatt gattcacaat gtgatctgtt
     3661 tgagagatta cgggatatag caaaagtcgt ctcaaccctg atttgggcta ttgttgcact
     3721 tcgagttgta ttaagcgcgt aggtgaatca tgtttggaat agtgttatct gctttgaact
     3781 cagttctggc atatctttta agaggtgttg tcttaaagtt cgttttattt acggctttgt
     3841 tttatgcagt aaaggagctt ttttcagttg tggctgatag gctcccccag tctggtcaag
     3901 ttgccgagtt gtttaataga ttaccggatg gcatgtggta ttttatttat tattttcagg
     3961 cggatattgg cattcctttg gtatttagcg cactctttac gcggtttatt attagacgtc
     4021 ttccgattat aggttaatta tggctatctc tgcatatgtt ggtgttccgg ggcatgggaa
     4081 atcttatgag gtcgtaggtt ctgttattct tcctgcttat ttggcgggta ggagaattgt
     4141 tacaaatatt gatggcgtaa aagaaagtaa cttttatgaa tacgccaaaa ggcataataa
     4201 ggatttaaat tcacttggca caattataaa ttgtacaaat gatgacgtat taagccctca
     4261 atttttgcct tataaagacg ctgaggaaga aacattttgc aaaagtggcg atttaatcgc
     4321 gattgatgag gcgtggcggt tctgggatag tgacaagaat atttgcaaag aacataaggc
     4381 gtttttcgct gaacatcgtc atatgtcaga taaagatact ttggcgacat gcgatattgt
     4441 tgtcattaac cagagccttg acacgatagc gaggtttatt aaaaccagaa ttgaaacaac
     4501 ctatagaatg cagaagcatg tttctgtagg cctaaagaat cgttatcgtg ttgatgtttt
     4561 tactggtgta aaactattta aaaactctcg ggtcacaagt tatcagaata aatataacaa
     4621 agaaatattt gctctatacc attcacatta cggcgggcaa ggtgatgaac aaacaactga
     4681 tgacaggcag aatattttta aacagaaaaa gctttgggtt ctgtttattt tcataataat
     4741 tatgctgtgt tcttcaggtt attttgtgtt ttcttttttt aagggttcaa cgcctgaaaa
     4801 taaagaggta agcactcccc aaaataaaca attaacagat aaggttacag ttcaaaataa
     4861 tgaagttgta ccgaaacaac cggtaattca aaagccggga ctttctaaaa ggtggagaat
     4921 taacggaact cttgttaaag aaggtaaaag ctttgtaatt ctcacggatt ctgtaggtct
     4981 gaatagattg gtctcaagag ctggtttttc aaatgatggc ttgatgctta acggagttgt
     5041 tgacggcgaa atcgtatctt actttagtgg cgaaaaatca tgaaaaagtt actttttttt
     5101 attctggttg tattctcttt accatctttt gcagtaaagg ttgatttccg tttggatgca
     5161 atgcagttgc caaaggctat caatttcttt tattcggatg taattaagca accgtttatg
     5221 cttgcgccag aactggcgaa cgatacgcga gttgtgtctt ttcatgtaac tgaagatgtg
     5281 gatgcaaaag cattctttgt aagatatctg agtaatatga atgtcgctgt tagtaacaaa
     5341 aatggcgttg attatattta tatggtgaca ccgaaacagg ttgatgtgcc taaatatact
     5401 ttcgtatata cgcctaagca tcgaagtgtt gcataccttt caagcctgct tcgggggatt
     5461 gttggcggcg attttggatc ggtagcagga gctgagccag tacagggtgt aactatgcaa
     5521 ccttctgtcg cccgaactgg gaatatcaat ggtgatggtg atgtgtttgt ctattatggt
     5581 acccggtcag atattaggaa aattgaggaa gtgttacctg tcatagatac cccatcctct
     5641 gaagtttatg tcggtggtta tgtctttgaa gtacagacca atgaacggaa tggatctggc
     5701 ttgcagctgg ccgccaagct tctgaatgac aaactctcga tccagattgg cggaactgct
     5761 gaaggcggtg gccagaactt tgttaaattc agtagcggta gtcttaatgc cctgtacgag
     5821 ctattcagga ctgacagtcg ctttcatgtg gtaagttcgc catccctcag agcgcgctca
     5881 ggggttcagg cttcgttttc tgtgggtgag gatgtacctg tactgggtaa tgtgtcttat
     5941 gagggtgata aggctgtaca gtcggtagaa taccgttcga gtggggttat ctttaatgtc
     6001 ttaccctatg ttcgctcaga tgtcatcgat ttgaacatca gccaggaact gtcgaatttt
     6061 gccaaaacag atacaggcgt aaacaattca ccaacgctca tcaagcgcag tatttctaca
     6121 gccgtatctg ttaaagacgg tgacataatt ctaattggtg gtcttgctga aaataagacg
     6181 aacgatacag atactgggtt gaccttcatg ccttcgtggt tttcgtctaa aagcagtgag
     6241 aagtacaaaa cagatattgt cgtcgtgtta caggcgagga aggtagcgcg gtaaggggga
     6301 agcccccgca gcttgcgagg aggcatcccc cttaccggga tgcctttttc gtttctaact
     6361 caaaggcaac tcggtgtgta tttcgtcaag ctgaaagacg gggggcccct ctggggttcc
     6421 gtttttctgc tttacgacac tccatttaag aaaacttttt atgggtgatg gatcacgtag
     6481 tgacccatcg ccctgtaaag ccggttttgt gtttttggtt tggatttttt gggcgttcgg
     6541 tcgcagcggg ttcagagtcc actctgttta atagtggact cttgtatcat tttgatatgc
     6601 tgttacaggc ctttttcttt taaaacgcct tcgatcgctt cttcaagttt atgttttata
     6661 aggtaattta ctagatctga ttgttttata ggcaagtttg cacgggttgt ggcttcaact
     6721 gttgcatttt tgagcagttc ggccatatgc ctgttgatag ttacagtggt tctcttgctt
     6781 gttccagaca taagatttat cctattgtta aaacttgttt acacgtcttg accattctga
     6841 cacatctggt acagtgtgga catggtgagt gtttgagtgg ttgaagtcat gatagatatg
     6901 ctggtattgc ggatcccttt taagccctat ctcgtaaa
//
