LOCUS       NZ_CP047642             8401 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Proteus sp. ZN5 plasmid pZN5-floR-8kb, complete sequence.
ACCESSION   NZ_CP047642
VERSION     NZ_CP047642.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13742196
            Assembly: GCF_011046025.1
KEYWORDS    RefSeq.
SOURCE      Proteus sp. ZN5
  ORGANISM  Proteus sp. ZN5
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Proteus.
REFERENCE   1  (bases 1 to 8401)
  AUTHORS   Peng,K.
  TITLE     The genomic epidemiology of tigecycline resistance gene tet(X)
            variants in a swine farm in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8401)
  AUTHORS   Peng,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) College of Veterinary Medicine, Yangzhou
            University, No. 567, Wenchang Middle Road, Hanjiang District, Yangz,
            Yangzhou, Jiangsu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011046025.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 02:10:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,065
            CDSs (total)                      :: 3,950
            Genes (coding)                    :: 3,881
            CDSs (with protein)               :: 3,881
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 69
            CDSs (without protein)            :: 69
            Pseudo Genes (ambiguous residues) :: 0 of 69
            Pseudo Genes (frameshifted)       :: 24 of 69
            Pseudo Genes (incomplete)         :: 52 of 69
            Pseudo Genes (internal stop)      :: 8 of 69
            Pseudo Genes (multiple problems)  :: 13 of 69
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047642.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8401
                     /organism="Proteus sp. ZN5"
                     /mol_type="genomic DNA"
                     /strain="ZN5"
                     /isolation_source="a nose swab sample of swine origin"
                     /db_xref="taxon:2697019"
                     /plasmid="pZN5-floR-8kb"
                     /geo_loc_name="China: Nantong, jiangsu province"
                     /lat_lon="31.41 N 120.11 E"
                     /collection_date="2018-05"
                     /collected_by="College of Veterinary Medicine, Yangzhou
                     University"
     gene            1..840
                     /locus_tag="GTK47_RS20310"
                     /old_locus_tag="GTK47_20245"
     CDS             1..840
                     /locus_tag="GTK47_RS20310"
                     /old_locus_tag="GTK47_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_165126853.1"
                     /translation="MATHKPIDILASFTELPPPIDYVLPNMVAGTVGALVSPGGTGKSM
                     LVLQLAAQIAGGPDLLDVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLTDEQRQVVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSGMTAAEAEE
                     WGVDDSQRPYFVRFGVSKANYGAPFKDRWFRRHDGGVLKPAVLEKQRKSKGVRRGEA"
                     /name="helicase RepA family protein"
     gene            827..1678
                     /gene="repC"
                     /locus_tag="GTK47_RS20315"
                     /old_locus_tag="GTK47_20250"
     CDS             827..1678
                     /gene="repC"
                     /locus_tag="GTK47_RS20315"
                     /old_locus_tag="GTK47_20250"
                     /function="putative replication protein"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_010895881.1"
                     /translation="MVKPKNKYSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFKGPEPLGADDLRILQGLVAMAGPNGLVLSPEPKTEGGQQLRLFLEPKWEAV
                     TADAMVVKGSYRALAREIGYAEDGGSQFKAIRECIERLWTVSIIAQNGRKRQGFRLLAE
                     YASDEAGGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSEAARLLHQRLCGWIDPGK
                     TGKAAIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            <1727..2182
                     /locus_tag="GTK47_RS20320"
                     /old_locus_tag="GTK47_20255"
                     /pseudo=""
     CDS             <1727..2182
                     /locus_tag="GTK47_RS20320"
                     /old_locus_tag="GTK47_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            2213..3097
                     /locus_tag="GTK47_RS20590"
                     /old_locus_tag="GTK47_20260"
     CDS             2213..3097
                     /locus_tag="GTK47_RS20590"
                     /old_locus_tag="GTK47_20260"
                     /inference="COORDINATES: protein motif:HMM:NF023271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3363 domain-containing protein"
                     /protein_id="WP_001447541.1"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRRG
                     VERVNCGPKLLDLGEKGVYSSYTCRVPGGDYEGYVDAHVRRLEALRRAGIVERIDADQW
                     RIPDDLVSRAAAHDAGRDSQASVRVLSPVDLNKQIGSDGATWLDRRLIHGETADLAPTG
                     FGQQVREAMDQRREHHIEQGDATRSRDSRVFYRRNLLAILREREVAGVGSDMALSKGLP
                     FRAATDGESVSGKFTGTVHLSSGKFAVVEKSHEFTLVPWRPIIDRQLGREVMGIVQGGS
                     VSWQLGRQRGLER"
                     /name="DUF3363 domain-containing protein"
     gene            3314..4528
                     /gene="floR"
                     /locus_tag="GTK47_RS20330"
                     /old_locus_tag="GTK47_20265"
     ARG             3315..4525
                     /name="floR"
                     /gene="floR"
                     /gene_name="chloramphenicol/florfenicol efflux MFS
                     transporter FloR"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4556..>4675
                     /locus_tag="GTK47_RS20595"
                     /old_locus_tag="GTK47_20270"
                     /pseudo=""
     CDS             4556..>4675
                     /locus_tag="GTK47_RS20595"
                     /old_locus_tag="GTK47_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /name="LysR family transcriptional regulator"
     gene            complement(4630..4980)
                     /locus_tag="GTK47_RS20340"
                     /old_locus_tag="GTK47_20275"
     CDS             complement(4630..4980)
                     /locus_tag="GTK47_RS20340"
                     /old_locus_tag="GTK47_20275"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_223424540.1"
                     /translation="MAFAIMRAKKLNSMGTVAAALQHCYRDRETPNADQERTPDNDHLA
                     ARSTDEAMGKLRERLPEKRRKDAVLAVEYVMSASPEWWQTASADQQREFFKRSTEWLAA
                     CRKFRCSATAMN"
                     /name="plasmid recombination protein"
     gene            complement(5440..5739)
                     /locus_tag="GTK47_RS20345"
                     /old_locus_tag="GTK47_20280"
     CDS             complement(5440..5739)
                     /locus_tag="GTK47_RS20345"
                     /old_locus_tag="GTK47_20280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010904468.1"
                     /translation="MAKTAERLAKLEEQRARINAEIQRIRAREQQQKRKENTRRKVLVG
                     AWILGKVENGEWPEQRLLDGLDSYLERDHDRALFGLPPKQKQQPQEHGQPEPQG"
                     /name="hypothetical protein"
     gene            5921..>6562
                     /locus_tag="GTK47_RS20600"
                     /pseudo=""
     CDS             5921..>6562
                     /locus_tag="GTK47_RS20600"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            6669..7079
                     /locus_tag="GTK47_RS20605"
     CDS             6669..7079
                     /locus_tag="GTK47_RS20605"
                     /function="putative role in conjugative DNA replication"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010895877.1"
                     /translation="MSAIDRVRKSRGINELAAEIEPLAQSMATLADEARQRIAEVQQAS
                     EEQAASWTSQQQQAMSAWRQAAKDMRAAAGELAKAGQTARSAARGWTWRLWAGVLIASV
                     MPILALLIASWLWLEPQIIEQQGSIWLIFKLK"
                     /name="IncQ-type mobilization protein MobB"
     gene            <7178..8032
                     /locus_tag="GTK47_RS20610"
                     /pseudo=""
     CDS             <7178..8032
                     /locus_tag="GTK47_RS20610"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /name="DNA-primase RepB domain-containing protein"
     gene            8100..8336
                     /locus_tag="GTK47_RS20355"
                     /old_locus_tag="GTK47_20290"
     CDS             8100..8336
                     /locus_tag="GTK47_RS20355"
                     /old_locus_tag="GTK47_20290"
                     /function="putative role in regulation of gene expression"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_010895879.1"
                     /translation="MDIGKAKEYFGLGLIQGAVVRSSSVLHNGWTIELSGNIGSAQPTL
                     HTARGDVRQFKTLDAAAKVVREIGLREWRVITD"
                     /name="hypothetical protein"
     Typing          5772..5859
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2..840
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.69"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5922..8029
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.573"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggctactc ataagcctat cgatattctg gcgagcttca cagagttacc gccgccgatt
       61 gactacgtac tgcccaacat ggtggccggt accgtggggg cgctggtgtc ccccggtggg
      121 accggcaagt ccatgctggt tctacagctg gccgcacaga tcgcaggcgg ccccgacctg
      181 ctggacgtgg gagagctgcc caccggcccg gtgatctact tgcccgccga agacccgccc
      241 accgctatac atcaccgcct gcacgcattg ggggcgcacc tgaccgacga gcaacggcaa
      301 gtcgtggctg acggtctgct aatccagccg ctgatcggca gcctgcccaa tatcatggcc
      361 ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat ggtgttggat
      421 acgctgcgtc ggttccatat cgaggaagag aacgccagtg gccccatggc gcaggtgatt
      481 ggccgcatgg aggccatcgc cgccgatacc gggtgttcta tcgtgttcct gcaccatgcc
      541 agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg gggatcgtcc
      601 gtgctggtgg ataacatccg ctggcagtcc tacctgtccg gcatgacggc cgccgaggcc
      661 gaggaatggg gcgtggacga ctcacagcgg ccctatttcg tccgctttgg tgtcagcaag
      721 gccaactatg gggcgccgtt caaagaccgc tggttcaggc ggcatgacgg cggggtgctc
      781 aaacccgccg tgctggaaaa gcagcgcaaa agcaaggggg tgcgccgtgg tgaagcctaa
      841 gaacaagtac agtctcagcc acgtccggca cgacccggcg cactgtctgg ctcccggcct
      901 gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga cgtatgacta
      961 cggcgacggc aagcggatcg agttcaaggg gccggagccg ctgggtgctg atgatctgcg
     1021 catcctgcaa gggctggtgg caatggctgg gcctaatggc ctagtgctta gcccagaacc
     1081 caagaccgaa ggcgggcagc agctccggtt gttcttggaa cccaagtggg aggccgttac
     1141 cgctgatgcc atggtggtta agggcagcta tcgggcgctg gcccgtgaaa tcgggtatgc
     1201 agaggatggc ggcagccagt tcaaggcaat ccgggagtgc attgagcgcc tgtggacagt
     1261 ctccatcatc gcccaaaatg gccgcaagcg gcaggggttc cggctgcttg ccgagtacgc
     1321 cagcgacgag gcaggcgggc gtctgtacgt ggccctgaac cccttaattg ctcaggccgt
     1381 catgggtggt ggccagcatg tgcgcatcag catggacgag gtgcgagcgc tggacagcga
     1441 agcggcccgc ctgctgcatc agcgcctgtg tggctggatc gaccccggca agaccggcaa
     1501 ggcggccata gataccctgt gcggctatgt ctggccgtca gaggccagtg gttcgaccat
     1561 gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggttgcgc tgggctggac
     1621 ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg caggctgacc
     1681 ccctgtttaa cagtcacgcc cctccccctc cgcactgtca gattgctacc agctcaagac
     1741 gccgtaccgg gacggcacca cgcacgtcat tttcgaacca ttggatttca ttgcaaggct
     1801 ggccgccctg gtaccgaagc ccagagtcaa cctaacccgc ttccacgggg tgttcgcacc
     1861 caacagtcgg caccgggcgt tggtcacgcc ggcaaaacgg ggcaggggca acaaggtcag
     1921 ggtggctgat gaaccggcaa caccagcaca acggcgagcg tcgatgacat gggcgcaacg
     1981 gctcaagcgt gttttcaata tcgacatcga gacctgcagc ggctgcggcg gcgccatgaa
     2041 agtcatcgcc tgcattgaag accctatagt gatcaagcag atccttgatc acctgaagca
     2101 caaagccgaa accagcggga ccagggcgtt acccgaaagc cgggcgccac cggctgagct
     2161 gctcctgggt ctgtttgact gacgagcctg aaggccaacg ataccaatca aaatgctgcg
     2221 ttcacagcgc cgcggcaggg atccgccgtg ctggttgtcg gaaaaggagc cgctagtggg
     2281 aaagaggagg gtaaattttc agcgttgctg gctccccgtc agccggattg ggttgcatcg
     2341 caggggtgtc gaaagagtca actgcggtcc aaagctgttg gacttgggtg aaaagggcgt
     2401 ttattcttcc tatacgtgcc gcgttccagg cggcgactat gagggctatg tcgatgccca
     2461 tgtgcgccgg ctggaggcgc tacgccgggc cggtatcgtc gagcggatcg acgccgacca
     2521 atggcgcatc cccgatgatc tggtcagccg tgccgccgcc catgacgccg gccgagacag
     2581 tcaggccagc gttcgcgtcc tttccccggt cgatctgaac aaacagatcg gatcggacgg
     2641 cgcgacctgg ctggaccggc ggctgatcca cggcgagacg gccgaccttg cgccaaccgg
     2701 cttcgggcaa caagtccgcg aagccatgga ccagcgccgc gagcaccata tcgaacaggg
     2761 cgacgccacc cgcagccggg acagccgcgt cttctaccgg cgcaaccttc tcgccatcct
     2821 gcgggagcgc gaggtagccg gcgtcggatc ggatatggct ttgagtaagg gcctgccgtt
     2881 ccgcgccgcc acggacggcg agagcgtcag cggcaagttt accggaaccg tgcatctatc
     2941 gagcggcaag ttcgccgtgg tcgagaaatc ccatgagttc acccttgtcc cgtggcggcc
     3001 gatcatcgac cgccaactcg gccgcgaggt tatgggcatc gtgcagggcg ggtcggtgtc
     3061 gtggcagtta gggcggcaga gggggctgga acgctgagtg cgcccatgcc gcattgcgaa
     3121 gcaaaagata atcggataaa atgtagcaat tcatattcgt aagcgtggag taatcagatg
     3181 ggaaattcca agtcagcaga caagtaagcc gcaacaacca gtattgttgt tgcggcgctc
     3241 tgtaaggcta gtctcatctg attgctgacg agcagacgtc gcccggtatt ccttaatcga
     3301 gggttgattc gtcatgacca ccacacgccc cgcgtgggcc tatacgctgc cggcagcact
     3361 gctgctgatg gctcctttcg acatcctcgc ttcactggcg atggatattt atctccctgt
     3421 cgttccagcg atgcccggca tcctgaacac gacgcccgct atgatccaac tcacgttgag
     3481 cctctatatg gtgatgctcg gcgtgggcca ggtgattttt ggtccgctct cagacagaat
     3541 cgggcgacgg ccaattctac ttgcgggcgc aacggctttc gtcattgcgt ctctgggagc
     3601 agcttggtct tcaactgcac cggcctttgt cgctttccgt ctacttcaag cagtgggcgc
     3661 gtcggccatg ctggtggcga cgttcgcgac ggttcgcgac gtttatgcca accgtcctga
     3721 gggtgtcgtc atctacggcc ttttcagttc gatgctggcg ttcgtgcctg cgctcggccc
     3781 tatcgccgga gtattgatcg gcgagttctt gggatggcag gcgatattca ttactttggc
     3841 tatactggcg atgctcgcac tcctaaatgc gggtttcagg tggcacgaaa cccgccctct
     3901 ggatcaagtc aagacgcgcc gatctgtctt gccgatcttc gcgagtccgg ctttttgggt
     3961 ttacactgtc ggctttagcg ccggtatggg cacctacttc gtcttcttct cgacggctcc
     4021 ccgtgtgctc ataggccaag cggaatattc cgagatcgga ttcagctttg ccttcgccac
     4081 tgtcgcgctt gtaatgatcg tgacaacccg tttcgcgaag tcctttgtcg ccagatgggg
     4141 catcgcagga tgcgtggcgc gtgggatggc gttgcttgtt tgcggagcgg tcctgttggg
     4201 gatcggcgaa ctttacggct cgccgtcatt cctcaccttc atcctaccga tgtgggttgt
     4261 cgcggtcggt attgtcttca cggtgtccgt taccgcgaac ggcgctttgg cagagttcga
     4321 cgacatcgcg ggatcagcgg tcgcgttcta cttctgcgtt caaagcctga tagtcagcat
     4381 tgtcgggaca ttggcggtgg cacttttaaa cggtgacaca gcgtggcccg tgatctgtta
     4441 cgccacggcg atggcggtac tggtttcgtt ggggctggtg ctccttcggc tccgtggggc
     4501 tgccaccgag aagtcgccag tcgtctaacc gacgactggt agcaggcccg ctccgatgcg
     4561 gcgcactaac catcgaaacc tcgtgaatgt cggtatcctg tctggcagga taccgctcat
     4621 ttcccttgtt cagttcatcg ccgtcgccga gcatctgaat tttcggcatg cggccagcca
     4681 ctcggtcgat cgcttgaaga actcccgctg ctggtcggcg cttgcggtct gccaccactc
     4741 cggcgaggcg ctcatcacgt attcgaccgc cagcaccgca tccttgcgcc gcttctccgg
     4801 caaccgctcc ctgagcttgc ccatcgcctc atcggtcgat ctggccgcca ggtgatcgtt
     4861 gtccggtgtg cgctcctgat cggcgttcgg cgtctcgcgg tcgcggtagc agtgctgcaa
     4921 cgcagccgcc accgtgccca tgctgttgag cttcttcgcc ctcatgatcg caaacgccat
     4981 ccgtcccccg gcgcaactgt cacgcccccg gcgtaactgt cacgcccccc cggcgtaact
     5041 gtcacgaacc cccggcgtaa ctgtcacgcc cctaaacctg caaacccagg aaggggggga
     5101 gctggtgggg tgttggaaaa atccatccat gattatccaa gaataatcca ctaggcgcgg
     5161 ttaacagcgc cctagtgggg cgctgtttgc ccttgcttaa aatgccggcc agaggccgga
     5221 taacttctct atccgctgcg ctaggctaca caccgccccc acccttcggg tagggaaaag
     5281 gccgctaaag cggctaaaat cgctccagcg tattcccgcg ggatttggta tggggtttag
     5341 cgggctttgc ccgcctttcc ccctgccgcg cagcgggtgg gggcggtgtg cagccccgaa
     5401 ggggccattc aggggccatt ctgaccgctt tgcgcttggc tatccttgcg gctccggctg
     5461 accatgctcc tgtggctgct gtttctgctt gggtggaagt ccgaacagtg cccgatcatg
     5521 gtcgcgctct aggtagctgt ccaacccatc gagcaggcgc tgttctggcc actcgccgtt
     5581 ttcaactttg ccgagtatcc atgcacctac cagcactttg cgccgggtgt tttccttgcg
     5641 cttttgctgc tgctcccgtg ctctgatccg ttggatctcg gcgttgattc gtgcccgttg
     5701 ttcttccagc ttggcgagcc gttccgctgt ttttgccatt ttgataatcc tcttggttat
     5761 gtgcttgtca ttatgctatc atcggagcac agacaccgca aaccccgacc gtcattttac
     5821 gggagggcgc acttaccggt ttctcttcaa gaaactgccc tgggggcacc ccttcggggc
     5881 tgtgcgctct ccgagggcca ttgcatggag ctaaacccct atggccatct atcaccttac
     5941 ggccaagacc ggcagccgga acggcggcca atcggccaag gcgaaagccg attacatcca
     6001 gcgggagggc cgttactccc gcgaccgtga agaggtgctg cacacccagt ccggccacct
     6061 gcccgagtgg gccgagcgtc ccgccgacta ctgggacggt gccgacctgt acgagcgggc
     6121 caacggtcgc ttattcaaag agattgagtt tgccctgccg gtcgaactga ccctagatca
     6181 gcagcgggag ctggtggacg aattcgcccg gcatctgact gagggcgagc gcctgccgta
     6241 tacgctggcc atccatgccg gtaacggtga gaacccccat tgccacttga tgatctccga
     6301 gcggaagaac gacggtatcg agcgcccggc tgaccagtgg tttaagcggt acaacggcaa
     6361 acagccagag cggggcgggg cgcagaagtc cgagagcctg aagcccaagg catggcttga
     6421 gcagacccgc gaggcatgga gccactacgc caaccgcgcc cttgaacagg ccgggcacga
     6481 ggcccgcatt gaccatcgca cccttgaggc gcagggcatt gagcgcttgc cgggcatcca
     6541 tcttggccct aacgtggtgg agatggagag ccggggcatc cgcaccgaga gggccgatat
     6601 cgccctagcc atcgacacgg ccaacggcca gatcatcgac ttacaagaat accgggaggt
     6661 tatcgagcat gagcgcgata gacagagtga ggaaatccag agggatcaac gagttagcgg
     6721 cagagataga gccgctggcc cagagcatgg cgacactggc ggacgaagcc cggcagcgga
     6781 tcgccgaggt acagcaggcc agcgaggagc aggccgcgag ctggacgagc cagcaacagc
     6841 aagccatgag cgcatggcgg caggcagcaa aggacatgag ggcagccgca ggggaactgg
     6901 ccaaggccgg ccagacggcc cggagcgccg cccgtggctg gacatggagg ttgtgggccg
     6961 gggtcttgat cgcttccgtg atgcctattc tggcgctgct gatcgcatca tggctttggc
     7021 tggagccgca aatcatcgag caacagggga gcatatggct gattttcaag ctcaagtgaa
     7081 aggtgaccgc accgcgcaag ccatcgcccg gcagctcaag gccatgggct gcgaccggta
     7141 cgacatcggc atacgggatg ccgccagcgg caagatgatg aaccgggaat ggacaccgca
     7201 ggaagtgcag cagaacgccg cctggctcaa gcgcatgaat gcccagggca acgatattta
     7261 cattcgcccc gccgagcagg cccggcatgg tctggtgcta gttgacgacc tcagcagcga
     7321 cgatctggac gccatgaagc aggagggccg ggagcctgcc gccatcatcg agaccagccc
     7381 caagaattat caggcatggg tgaaggtggc tcaggatgcc ccagcagatc ataggggcgt
     7441 aatcgcccgg aagctggccc gggagtatga cgccgacccg gcaagcgccg acagccgtca
     7501 ctatggccgt ctggcaggct ttaccaaccg caaggacaag tacaccagcc gcaccggcta
     7561 tcagccgtgg gtgctgtgcc gggagtccag cggcaagagt gccaccgcag ggccggagct
     7621 gatgcagcag gccgggcagg tgttggacag catcgagcgc cgacaggaac ggacggcacg
     7681 actggccgag atcaccgccc cgcagagtgt gcgtcggtat cgccgcagcg tcgtggacga
     7741 ctaccgcagc gaaatggccg ggctggtcaa gcggtacggt gatgacctca gcaagtgcga
     7801 cttcatcgcg gccatgaagc tggccagcaa gggccgggag ccggacgaga tcagcaaggc
     7861 catggccgag gctagccccg ctatcatgga gcgcaaggcg ggccatgagg ccgattacat
     7921 caagcgtacg gtgcagaagg tgatggagct tccgcaggtg caggaggccc gcgccgagct
     7981 ggcgaagcag acccagaagc agcgcagcaa ggggccagat ttaagcatgt aatgcttgtt
     8041 ttgataaaac aaaactgcta tattgtcaaa acaatttgac tttatagtag gattaaaaaa
     8101 tggatatagg aaaggcaaaa gagtattttg ggcttggatt gattcagggt gccgtggtga
     8161 gatcgtcaag tgttctgcat aacgggtgga ctatcgagct gagcggcaac attggcagcg
     8221 cacaacctac cttacacacc gccagaggtg atgtgcggca gttcaagaca ctggatgcgg
     8281 cggccaaggt cgtgcgagaa attgggctgc gagagtggcg tgttatcact gactaggcgg
     8341 cgcatggcgg ggatgccccg ccagcgcctg accatcaacc gcctgcaaag gaggcaaacc
     8401 a
//
