LOCUS       NZ_CP047638            10077 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain K2606 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP047638
VERSION     NZ_CP047638.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13831363
            Assembly: GCF_011006575.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10077)
  AUTHORS   Li,J., Xu,Q., Li,Z., Wang,P., Xie,Q., Sheng,Z. and Wang,M.
  TITLE     Activated efflux pump AcrAB confers decreased susceptibility to
            piperacillin/tazobactam and ceftolozane/tazobactam in Klebsiella
            pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10077)
  AUTHORS   Li,J., Xu,Q., Li,Z., Wang,P., Xie,Q., Sheng,Z. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2020) Infectious Diseases, Shanghai Jiao Tong
            University School of Medicine, 197 Ruijin Er Road, Shanghai 200025,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.13.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 308.0x
            Sequencing Technology             :: PacBio RSII
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 07:15:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,724
            CDSs (total)                      :: 5,601
            Genes (coding)                    :: 5,448
            CDSs (with protein)               :: 5,448
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 153
            CDSs (without protein)            :: 153
            Pseudo Genes (ambiguous residues) :: 0 of 153
            Pseudo Genes (frameshifted)       :: 72 of 153
            Pseudo Genes (incomplete)         :: 88 of 153
            Pseudo Genes (internal stop)      :: 28 of 153
            Pseudo Genes (multiple problems)  :: 27 of 153
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047638.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10077
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="K2606"
                     /isolation_source="blood"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China:Shanghai"
                     /collection_date="2012-06"
     gene            complement(join(9851..10077,1..202))
                     /locus_tag="GUY22_RS29085"
                     /old_locus_tag="GUY22_28030"
     CDS             complement(join(9851..10077,1..202))
                     /locus_tag="GUY22_RS29085"
                     /old_locus_tag="GUY22_28030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(366..680)
                     /locus_tag="GUY22_RS29090"
                     /old_locus_tag="GUY22_28035"
     CDS             complement(366..680)
                     /locus_tag="GUY22_RS29090"
                     /old_locus_tag="GUY22_28035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120406.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001136729.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKAGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYQELVIGFGDSGYVMLYRHDREMDRIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(668..946)
                     /locus_tag="GUY22_RS29095"
                     /old_locus_tag="GUY22_28040"
     CDS             complement(668..946)
                     /locus_tag="GUY22_RS29095"
                     /old_locus_tag="GUY22_28040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(1288..1506)
                     /locus_tag="GUY22_RS29100"
                     /old_locus_tag="GUY22_28045"
     CDS             complement(1288..1506)
                     /locus_tag="GUY22_RS29100"
                     /old_locus_tag="GUY22_28045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002193751.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_002193751.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHFFFGKR"
                     /name="TonB family protein"
     gene            complement(1503..1874)
                     /gene="tolA"
                     /locus_tag="GUY22_RS29105"
                     /old_locus_tag="GUY22_28050"
     CDS             complement(1503..1874)
                     /gene="tolA"
                     /locus_tag="GUY22_RS29105"
                     /old_locus_tag="GUY22_28050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(2148..2393)
                     /locus_tag="GUY22_RS29110"
                     /old_locus_tag="GUY22_28055"
     CDS             complement(2148..2393)
                     /locus_tag="GUY22_RS29110"
                     /old_locus_tag="GUY22_28055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159210150.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNRTLPKEVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPLRRR"
                     /name="hypothetical protein"
     gene            2720..4405
                     /locus_tag="GUY22_RS29115"
                     /old_locus_tag="GUY22_28060"
     CDS             2720..4405
                     /locus_tag="GUY22_RS29115"
                     /old_locus_tag="GUY22_28060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            4415..4672
                     /locus_tag="GUY22_RS29120"
                     /old_locus_tag="GUY22_28065"
     CDS             4415..4672
                     /locus_tag="GUY22_RS29120"
                     /old_locus_tag="GUY22_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            4757..4906
                     /locus_tag="GUY22_RS29125"
                     /old_locus_tag="GUY22_28070"
     CDS             4757..4906
                     /locus_tag="GUY22_RS29125"
                     /old_locus_tag="GUY22_28070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            6389..8350
                     /locus_tag="GUY22_RS29130"
                     /old_locus_tag="GUY22_28075"
     CDS             6389..8350
                     /locus_tag="GUY22_RS29130"
                     /old_locus_tag="GUY22_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_159210147.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            8350..9081
                     /gene="mobC"
                     /locus_tag="GUY22_RS29135"
                     /old_locus_tag="GUY22_28080"
     CDS             8350..9081
                     /gene="mobC"
                     /locus_tag="GUY22_RS29135"
                     /old_locus_tag="GUY22_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_159210148.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQISVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEGRGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKVNGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            9088..9618
                     /locus_tag="GUY22_RS29140"
                     /old_locus_tag="GUY22_28085"
     CDS             9088..9618
                     /locus_tag="GUY22_RS29140"
                     /old_locus_tag="GUY22_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_159210149.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMAVMAWQTGAYVFRFE
                     DQAQHLAMMKFIGMYHERLNSDDKATYYFTGKNPAWEREKPAGMKLCDYLYRKVEQFAI
                     ENGVQVLDAGKYTPEQIGAMIDVVSERYGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRRWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9646..9825)
                     /locus_tag="GUY22_RS29145"
                     /old_locus_tag="GUY22_28090"
     CDS             complement(9646..9825)
                     /locus_tag="GUY22_RS29145"
                     /old_locus_tag="GUY22_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_065521055.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMQDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     BGC             1..9405
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(5010..5588)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.11"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8351..9078
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="9.26e-141"
ORIGIN
        1 ggtgtgaaat gccgggaatt tgtttctcag ggatattcac atttccgtat acacggatca
       61 gttctgacac atccacgaca gggttgtttt tcccgtcacg tgacacggta acctttccct
      121 gttttacatg attgtatagc gttcttctgg taatgcccgc ggcttgtgct gcctgtgaaa
      181 ggttgagcaa tgttttcgcc actgtatacc cctgatttta tcgggtgtga aaggtgtgta
      241 atttctcacg gtaaatgtat cgctgaattt tacccgtttt tccgttcagg atgtggtttt
      301 ctgacgcgct gtgcttgtca aaccggcgac ggccacgcaa tgaattgcgc ccccgtcatc
      361 ggttattaga ggctgtctgc ccccggatac ccggattctt tctgatgcct gacggtgacg
      421 atcacaatcc ggtccatttc ccggtcgtgg cggtagagca tcacatagcc actgtcgcca
      481 aacccgatca ccagttcctg atattccagc ggcagaaatg gcaccgggcg gccaatgtct
      541 ggcaatgttt tcagttgctg gatagcccgg acgatcacct caccggcttt tctggctgcc
      601 agccgatttt tggttttgag aaagtcctgg aggcgttgta aatctcgctg tgccagcgcg
      661 gaaatcgtta cctgtggcat ggcggagcat cctgctcgtt ctcagttccc caggtactta
      721 tccaggcttc tgcttcttcc gcagtaaggt gcagacccgt ttcctgatag tgctgccatg
      781 cggcttgtcc gtcccgcagg tactggctgc gcttctcttc ccggtcgatg tattcggtga
      841 ttgcctccag cattagcgcg tgcgctgagc ggtgccggtc atcggccagc gttttcagcc
      901 ggtccttaag ttcttcatcg agcctgatgg atgttgctga tgacataaaa gcccctttgt
      961 agtcatgtgt aatacatatg actactttaa tccgtggcgg cgctatttgc cagaaatacg
     1021 gcttttgcta cccatttttc agcttcgctt ccccattttt cagcttcgct tccactaatc
     1081 agggctgcta caggctcact catcatccag gactattgca gcctgcgggt taaaggacct
     1141 gttactggcg gcaaccatca tcattgaact ttttggtggt ggctggtcgc ggatcctgta
     1201 agccgtcatt tttcagactg aattcaaaat ttataacctg tcgcattgcc gggtgccctg
     1261 cgggatatag ccagtgcctc attgccgtca gcgtttcccg aaaaaaaagt gacagccggt
     1321 aagcgacacg ttgtccactc gtccatcgtc cagaatatca aacgtggcgg tcacctgacc
     1381 ttcgatgcgt tttgctgcgg cctgtaccgg gtaggttggc tgcagtgtgg ttacgggttt
     1441 tagtggtatc gcatgtaccg gagtgaggaa catggccgcc agcactgtca gagtaaaagc
     1501 tttcatggtc tgaaatccag aggggcgttt ttgaatattt gccaggtatt ttcatccggt
     1561 gccggcggaa ttttggcgtg ggtaacggct gatattgctg ctttacagag tttcggatct
     1621 cctccttcag caatggcgct gttcagagtg ccatcacgtt gtatggatat ccgcacggtg
     1681 cacatttttc ccgcataggc ctctggttca aacatctgca tttcaacagc ggtcttgatg
     1741 gccgcagcgt agctactgat tttagatgac ttatccagag tataaatttt cggcggctgg
     1801 gtggtggaat gcttacaggc cgtcaggagt aaggggctgc acaatgccag caggataaag
     1861 ggtattaaac gcatcgggtt gatgacctgt cagtaaaaaa tattacatgt atatttgcca
     1921 ctctttacgg ccacgttcat tgacataaac acggcttatc gtatcgtttt tacaccaaac
     1981 ggcccgccat gcagttgcgt gcataggcat gcagcgggtt acaatttacc gggcgcgttt
     2041 ccggcggttt tccgggtgat ttgttgcctg ttttatcccg tagccgccgg aaacgctctg
     2101 agggcttctc gggcggtgct gataacgggt tttatggtaa atacccctca ccggcggcgc
     2161 agcggctgcc aggtgaaggt ggtttccgcc gcctcgtatt tctcccgacg catttcctgc
     2221 gcccgcctgg cttcacgttc gtcctgaatg gcgcgaagca ccctttcccg cacctgctgt
     2281 tttacctcct ttggcagcgt tctgttccct ttcaggccca tggccagtgc ctgagcgaca
     2341 aaggcgtccg ccaggcgctt ttcttcagcg ggagtcagtt tcaggtgtgt catggtggcc
     2401 tccgggcgtc tcaggtttcc tgaacagttt aaccgcgggt cctcccctca taccagttag
     2461 ctttcagatg tgtctatacc acctgaaatg taagtgtgat gtacatctca aataataaaa
     2521 tttgttgccg tgagaaattt tcgggaaaag acgcgatctg cttacaggtg gctccgggcg
     2581 cttttttgtg cctgaagatg agaactaatc tcatatattg acacctgaaa acgggaggag
     2641 taaggtaata attatactgt gtatatgtac agtatgttgg ctttaattta ttgttttaaa
     2701 tgtcaaagag gaaaacgata tgagtggtgg agacggtcga ggtccgggta attcaggtct
     2761 gggacataat ggtggtcagg ccagtgggaa tgtgaacggt acgtctggta aaggtggccc
     2821 ttcatcaggt ggtggtacgg atccaaacag cgggccgggc tggggtacga cgcatacgcc
     2881 gaatggagat attcataact ataatccggg ggagtttggt aacggcggga gtaaacccgg
     2941 tggtaatggt ggtaacagcg gcaatcatag cggtagctct ggtggcggac agtcttcggc
     3001 cactgcgatg gccttcggtc tgcctgccct ggctactccg ggggctgaag gactggcttt
     3061 atcagtttcc ggcgatgcgt tgtcggccgc cgttgctgat gtgctggctg ccctgaaggg
     3121 gccgtttaag tttggtctgt gggggattgc gatctacggt gtgctgcctt ctgagattgc
     3181 aaaagatgat ccgaaaatga tgtcaaaaat tatgacgtca ttaccggccg atgcggtgac
     3241 ggagactccg gcaagtactt taccactgga ccaggcgacg gttcgtgtca gacaacgggt
     3301 tgtggatgtg gtgaaggatg agcggcagca tattgcggtt gtcgcaggtc ggccaatgag
     3361 tgtccctgtg gtggatgcga aaccgacaaa acgtccgggg gtattcagtg tgtcgattcc
     3421 gggtctcccg tctctgcagg tgagcgtacc gaaaggtgtt ccgacagcga aagccccgcc
     3481 aaaaggcatt gttgctgaaa aaggtgattc acgtccggct ggttttacag ccggtggtaa
     3541 ctcccgtgag gccgttattc gtttcccgaa agagaccgga cagaagccgg tttatgtgtc
     3601 ggtgacagat gttcttaccc cggcacaggt aaaacagcgt caggaggaag aaaagcgtcg
     3661 ccagcaggca tgggacgccg ctcatccgga agaggggctg aaaagagact atgataaagc
     3721 gaaagctgag ctggatgccg aagataaaaa tattgcgacc ttaaacagcc gcattgcatc
     3781 gacagagaag gcgctccccg gtgcaagggc tgctgttcag gaagccgata aaaaggtaaa
     3841 agaggcagag gcaaataaag atgattttgt gacttataac ccgcctcatg aatatggctc
     3901 cgggtggcag gatcaggttc gctatcttga taaggatatt cagaatcaga atgagaaatt
     3961 aaaagcggct caggcatctt taaacgcaat gaatgaatcc ttatccagag ataaggctgc
     4021 acttaccggg gcgatggaga gccggaaaca aaaggagaaa aaagcgaagg atgcagaaaa
     4081 taagttaaat gaggaaaaga acaagcctcg caaggggact aaagactacg gccatgatta
     4141 tcatccagta cctaaaacag aagacataaa gggacttgga gagttaaagc gaggtgatcc
     4201 taaaacacct aaacaaggtg gtggtggtaa gcgggcccga tggtatggcg ataaaaaacg
     4261 taaaatttat gaatgggatt cccagcacgg tgagcttgaa gggtatcgtg ccagtgatgg
     4321 cgaacacctc ggggcatttg atcccaaaac gggtaagcag gttaaagggc cagatccaaa
     4381 acggaacatt aaaaaatatc tttgagaggt aaatatgggg cttaaattac gtttgacatg
     4441 gtttgataag aaaactgaag agtttaaagg ggaagagtat tctaaagact ttggtgacga
     4501 tggttctgtc attgaaagct tgggaatgcc tttaaaggat aatattaata atggtgagtt
     4561 tgatttgaag aaagaatggg ttcctttact acagccattt tttaaaaatc aaataaaacc
     4621 tgatgtggat aattgttata tagcttttga ttatcgggac ggtaactggt aacaaaaaat
     4681 tatcaaggat gatttgaaat actgacttcc ggtgttcagg gatgaatgcc ggagtcttta
     4741 aatacaggga aagtatatga aaaaggtaaa gactattttt ctttttatat tgattgttgc
     4801 tggctttctt ctggccgcat gtcaggcaaa ttatatccgg gatgttcagg gcggaacggt
     4861 ggcaccatcg tcctcctctg aactgacggg gatcgcggtt cagtagaaaa gatcaaagga
     4921 tcttcttgag atcctttttt tctgcgcgta atcttttgcc ctgtaaacga aaaaaccacc
     4981 tggggaggtg gtttgatcga aggttaagtc agttggggaa ctgcttaacc gtggtaactg
     5041 gctttcgcag agcacagcaa ccaaatctgt ccttccagtg tagccggact ttggcgcaca
     5101 cttcaagagc aacctcgtgt ttagctaaac aaatcctctg cgaactccca gttaccaatg
     5161 gctgctgcca gtggcgtttt gcgtgctttc ccgggttgga ctcaagtgaa cagttaccgg
     5221 ataaggcgca gcagtcgggc tgaacgggga gttcttgctt acagcccagc ttggagcgaa
     5281 cgacctacac cgggccgaga taccagtgtg tgagctatga gaaagcgcca cacttcccgc
     5341 aagggagaac ggcggacagg tatccggtaa acggcagggt cggaacagga gagcgcaaga
     5401 gggagcgacc caccggaaac ggtggggatc tttaagtcct gtcgggtttc gcccgtactg
     5461 tcagattcat ggttgagcct cacggctccc acagatgcac cgaaaaagcg tctgtttatg
     5521 tgaactctgg caggagggcg gagcctatgg aaaaacgcca ccggcgcggc cctgccgttt
     5581 tgcctcacat gctcgttccc tgcctatcca cggaatctgt gggtaacttt gcgagagttc
     5641 gcagcgcccg ccgcagtctc acggccggcg cgtagcgacc gagtgagcga ggaggcgcaa
     5701 tttccccggc acctgcaaga cggtcaccgg acgcgtggat gtgacatatt gctccgtgtt
     5761 accgcctttc tcttccctgc cgataaatta ttcagatagg tgaagtctgt cggttttctg
     5821 ctgaatattt tacgttaacc tatctctgtt gcttttttct ccttgcggag gaatgcacgt
     5881 catccccgct ggggcgtgac tgacttcagg gggaaatccc ctgcttacgc tggctggacg
     5941 ccaccgacgc actgtctttc cccctctttc tggctctgtc cggctggacg ccggtcacgc
     6001 cagaaattca cccggccccc gtcaggctca cgcctgacca cccacaaaat ccacggtgct
     6061 ggacgcacct gacgaagcag caaagctgct cctgattttg cgggtggtca gccgctcgct
     6121 atggcgaact ctgacggggg ccggaacacc ggaaaagacc gcactggggt gccgcctttt
     6181 cctctcacac gctggggcgt gctcgttgtt cctccggggc tgcgcctttt ccgcatgggg
     6241 cggaaagagg ctggcctccg cggtgaaagc tgaacgcaat caccacggac gtgaccgccg
     6301 ccgccatgcc ttggggcatg tccgcggctt gtgcagcagg ggctgcacaa cacgccggta
     6361 aacccgtcgt ctgaaggaga gagattgcat gtttaatacg gattcgcttg cctggcagtg
     6421 gcatatcccg gagctggccc ggcagattta cccgtgggtc agctggctgt accgggagta
     6481 ttacagtctg gccggttttt ttctggttat cggcctgttt ttcggtctgg cagaactctg
     6541 ggccgggcgg atgcgattcg tggggctgag tcataaggag cggcgggagt atgccgatcg
     6601 ctataagcgc gataataacc ggactgcgcc attttcggtg ctggcctcgc tggattcgcc
     6661 cgggcggatg gtcagggcac ggcgacatgt ctggaactac gcatactggc tgaccggcgc
     6721 tgccggtctg gcccttctgg gggggctggt tgtcgccgtg tttatcgaga gctggggaga
     6781 aaccgtcctg cgtccggacc tctacgcgaa cgcagattat tacctgctgc agccggctaa
     6841 cctgttcggg attgcctggt gtgccggagg tcttgtcgca ggcgtcctcc tggcgcggct
     6901 gctggcgttc ctgctgcttg acgggcattt tgcggccgct gacgaggcgg tgaacgcgaa
     6961 actgaatcag gagagccggc gcagcagcca gcggaccgga gaaatgaccg acgtccgcca
     7021 cctgcatttc ggggagccgg taccggttaa cgcgctggcc gatttttcca cggagcaggc
     7081 ccggaaacag caggcggtgt ttctgggtaa ggatgagcag ggccagccgg tgctggtccc
     7141 gcgtgatacc tggcgcaaga ccaacattca gatcctcggc ctgcccggga gcggcaagag
     7201 cgtgatggcc accaacgcgc tcattcgctg cgtacgcgat ttcggggacg cggtggtgta
     7261 cttcgacccg aaaggggacg cgtgggcgcc gcacgtcttc cgggcgcact gcccggactt
     7321 caccctgctg gatctgcggc cggggaaacc ggcgcagctg aatctgttcc gcgatctcga
     7381 ccagtacgcc ctgaagaatc ttctggttgc cggatttaac ctgagtgaga ccggcgacgt
     7441 ggcggaccac taccgcatca gcgagcagaa agcggcgaag ctgattgcgg agcagtttcc
     7501 gcagggggca aatattcagc aggtgctggc ggcggcgtac gcactgccgg aggccctgaa
     7561 aaaggacgtg aaggggctga tcaccaagct ggagaacgtg gccgacctga gcgtccttca
     7621 gacggacagc ggcatcgacg tggcggggat aatcaacggc ggcggctgcc tgtacgtcat
     7681 cggctcgatg gacgatgagg cggtgatccg ggtgcagaag atgctgtttg cccgctgcgc
     7741 gcaaatcatc attgcgcggg acgagttccg gaggtggccg cacgcgagca tcatgctcga
     7801 tgagattaaa tatctgctgt cgaagtacgt gctgaacgcg ctggggacgc tgcgcagccg
     7861 ggactgcaac ctgctgctcg cgcaccagtc gctgggcgac ttcggccagt gcgggcagga
     7921 tctgcccgct gattttgtga aaaccacggt actggataac acgccgatcc gctggttcta
     7981 ccgggcggcc agccaggagt ccgctcagtg ggcggccgga cagaccgggg agatccgggt
     8041 ggacgtggag cgccgccggg ccagccggga ggcggggaac gtggagcata tcagcgggga
     8101 cagcttcatc cagaaggagg cgcggccgct gtttgacgtg aacaccctgc aacatctgcc
     8161 tgacgggttc gcggtgatga ccgggctggg cgtggcgcgg ctggcgttca gcagcccgct
     8221 gcgtgttgat cgccgggaaa ttcctctgaa aaccttcccg gtgctggcga aaacggaccc
     8281 gctggctgag taccagccgg aggccgggcg gcagcggccg ggtgatgatg attttgcggg
     8341 gctgtactga tggcactgga gcgatacaac gtcagccacg cgaagcggca ggcgcggaac
     8401 gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag agctgtgttc cacggcggag
     8461 ctggttgcgc gacgtctggg gattgcggcg gtacagccgg tttaccgctt cctggacagc
     8521 ctggtggcga aagggctgct ggtccgggcg aaatacccgg ttgacggccg tcagatcagc
     8581 gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg acgaggacga gccgctgact
     8641 gacattattc cgtttcagcc gtcgcgggtg tcggcggcgc agctgccgca ccggctggcc
     8701 gtgcagtccc tgcgcctcgc catggagggc cggggggcca ccggctggcg gtacctgcac
     8761 cgtattgccc tgaaagggat gaaggtgccg gacgcgctgg cggagctgga cggcagaacg
     8821 gtggcgtttg aggtggaacg taccgtcaag agccgtcgcc ggtatcagga agtggtgtcc
     8881 gggtatctgt tcaaccgtaa ggtgaacggc atcgatgaaa tctggtacat ctgccctgac
     8941 cggccgacgc ttgtccgggt acagcgggcg attctggcgg tggatgaaat cgtgaacccg
     9001 cagaccggcg aggcccgcaa aacggcggag ctggaccggg agaggctgtt cgcatgcttt
     9061 aaatttatga caacggagtg agggattatg gcgctgtaca tggtatttat tgaagacgcg
     9121 gaggaccggg acggcgagcg ctgcattatg ccaaccagcg agcatctgga agacatggcg
     9181 gtcatggcct ggcagaccgg tgcatatgta ttcaggtttg aagatcaggc ccagcatctg
     9241 gcaatgatga agtttatcgg catgtatcac gaacggctga acagcgatga caaagccacg
     9301 tattacttca ccggcaaaaa tccggcgtgg gaacgtgaga aaccggccgg aatgaagctg
     9361 tgtgattatc tttatcgcaa ggttgagcag ttcgccatcg agaacggcgt gcaggtactg
     9421 gacgccggga aatacacccc ggagcagatc ggggcgatga ttgacgtcgt gagtgagcgg
     9481 tacggggaaa gcgcggtgaa cgagaagctg aaagcggagc tgatgcggga atataacagc
     9541 cgctatttta acgttccggt gccagaactg ccggaggata aacccgcccg ccggaggtgg
     9601 ccgttcggca aaaaataaac gccagcaccg gccatgccgg tgctgttatt cttccccgag
     9661 cttatcctgc atcacctgcc agagtgtttc cgcatcctcg tgcagccgct cgcacatgtc
     9721 ggccggggta tccatatcca gctcgttcag tttctcgagc aacagcagcg actggtcctt
     9781 aatgaattta gccatattga gggcggtttt ttcctgcttc gtcatgttct ccattccctt
     9841 cggtaagttc tcagctaaac cagccggacc agaagcgttt tttcttctgt gtcagttctg
     9901 ttgtcaatct gtccacttcg gcctggagtt tccggcgctc atcatcgtgc tggcggcgtt
     9961 cttcttctcg ggatgtttta tcttccagca tcagggtgac agcctgccgg agatcggcca
    10021 gttccttttg cattgccaga agctgttcct gtgggaaatt tttttgtgta ttttcac
//
