LOCUS       NZ_CP047582             1506 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 94EC plasmid p94EC-6, complete sequence.
ACCESSION   NZ_CP047582
VERSION     NZ_CP047582.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13756518
            Assembly: GCF_009905135.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Ding,Y. and Seedorf,H.
  TITLE     Emergence of Tet(X4)-producing Enterobacteriaceae in Human Gut
            Microbiota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Ding,Y. and Seedorf,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2020) Henning Seedorf Group, Temasek Life Sciences
            Laboratory Limited, 1 Research Link National University of Singapore
            Singapore, Singapore 117604, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-OCT-2019
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 433.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009905135.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 02:15:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,163
            CDSs (total)                      :: 5,040
            Genes (coding)                    :: 4,840
            CDSs (with protein)               :: 4,840
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 80 of 200
            Pseudo Genes (incomplete)         :: 126 of 200
            Pseudo Genes (internal stop)      :: 43 of 200
            Pseudo Genes (multiple problems)  :: 42 of 200
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047582.1.
            Bacteria and source DNA available from Henning Seedorf, Temasek
            Life Sciences Laboratory.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="94EC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p94EC-6"
                     /geo_loc_name="Singapore"
                     /collection_date="2019-02"
     gene            1..441
                     /locus_tag="GUJ28_RS25680"
     CDS             1..441
                     /locus_tag="GUJ28_RS25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     Typing          complement(727..983)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.186"
                     /coverage="98.0"
                     /evalue="3.06e-107"
ORIGIN
        1 atgagtaagg atgtcgagag aatcactcag ataaatgacc agggtgagat tatcggcggc
       61 ttcgttgccg ttatccgccc taaacaaaaa tcagcatttc agaggcattt caccatgaat
      121 caagacgcat tacggatctt ggctaaggaa ttaaccgggg agcaattcaa ggtcttaatg
      181 ctgatgcttg cagatctgga ttacgaaaac ttcattcaga tcgcccaagc agatattgct
      241 gaaactttag ggatgcagaa aacgaatgtt agtcgcgccg ttcgcgcgct actggatgtt
      301 ggggtgatct ttgagggacc taaagttggc agatcaaaaa cataccggct gaatgaacaa
      361 tttggttgga aaggcaccgt aaccaaccac aaaaaggcac tcaaaaacgg ctttagtgtc
      421 attcagggcg gccggattta aagagccagc ggcaggcgga caatcagggc tacgtgttaa
      481 cgttttgacc atgattgtct atcctgcatt gctcttttgc cgcatgtcag ttcgatgcga
      541 tgcaggatag ggcaaacgcc gcaaaatgac gtcattgacg tcattcgcaa cctttgcccg
      601 ataatctcca aaacgtctga gctgccacgg ttccggcagc gccttgagcg caaccggaaa
      661 ccgtctgggg agaaccccag acccccatcg ctgtaacatc gacggtctac gaccaccaac
      721 cagctgaata gctgccagtg ccgtgcaaat gggctttgca gggggctgac gcggttcctg
      781 gcgacctgaa agcccctggt taggggtatc ggcgcgcctg acctcctgaa accctctgta
      841 agcgtttttc gcgccttcca tgccctgacg ggcatttaaa acccgtttcg tgcgcctgag
      901 agcttctgag agcgtttgaa gagctatctg gcgactcatc cccgaaagaa caacagcgga
      961 atcaccgata acatgcgaga ggtggcaccg ctcgccgcag ccccagcgac cgaacgttag
     1021 tgagcgagtg ggcgaggaag cggaagagaa ccggaaacca cattgagcac gtacgcactg
     1081 atgcggggtg tcggggtgaa gccctgacca gatggcaaac ggaatatcgt cgcgggtgac
     1141 ggtattacat ttgcacatcc tgtcccgatt tctgcggagt ttaacggtga acggacggca
     1201 aaaaacgtgc aggagctgtt gaacgtgaaa acctcgaaat ctggcaaaaa tcacggctga
     1261 cgaggcttgc agaggattaa gccagtatac actccgctat cgctacgtga ctgggtcagg
     1321 gcttcgcccc gacaccccaa agagtagcta ggataaatat ctaatggaat caatgcgtta
     1381 gacgcctaat ataaagatac agaaaggaga ataaaaacat taacaaaatc taagggttac
     1441 agttgacata gttatcaata atgataacac tgttatcatt attgataact caaatagggt
     1501 tcaaag
//
