LOCUS       NZ_CP047581             6647 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain 94EC plasmid p94EC-5, complete sequence.
ACCESSION   NZ_CP047581
VERSION     NZ_CP047581.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13756518
            Assembly: GCF_009905135.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ding,Y. and Seedorf,H.
  TITLE     Emergence of Tet(X4)-producing Enterobacteriaceae in Human Gut
            Microbiota
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Ding,Y. and Seedorf,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-JAN-2020) Henning Seedorf Group, Temasek Life Sciences
            Laboratory Limited, 1 Research Link National University of Singapore
            Singapore, Singapore 117604, Singapore
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 03-OCT-2019
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 433.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_009905135.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 02:15:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,163
            CDSs (total)                      :: 5,040
            Genes (coding)                    :: 4,840
            CDSs (with protein)               :: 4,840
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 200
            CDSs (without protein)            :: 200
            Pseudo Genes (ambiguous residues) :: 0 of 200
            Pseudo Genes (frameshifted)       :: 80 of 200
            Pseudo Genes (incomplete)         :: 126 of 200
            Pseudo Genes (internal stop)      :: 43 of 200
            Pseudo Genes (multiple problems)  :: 42 of 200
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047581.1.
            Bacteria and source DNA available from Henning Seedorf, Temasek
            Life Sciences Laboratory.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="94EC"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p94EC-5"
                     /geo_loc_name="Singapore"
                     /collection_date="2019-02"
     gene            complement(join(6604..6647,1..376))
                     /locus_tag="GUJ28_RS25425"
                     /old_locus_tag="GUJ28_25425"
     CDS             complement(join(6604..6647,1..376))
                     /locus_tag="GUJ28_RS25425"
                     /old_locus_tag="GUJ28_25425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(457..894)
                     /locus_tag="GUJ28_RS25430"
                     /old_locus_tag="GUJ28_25430"
     CDS             complement(457..894)
                     /locus_tag="GUJ28_RS25430"
                     /old_locus_tag="GUJ28_25430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1154..2722
                     /gene="cea"
                     /locus_tag="GUJ28_RS25435"
                     /old_locus_tag="GUJ28_25435"
     CDS             1154..2722
                     /gene="cea"
                     /locus_tag="GUJ28_RS25435"
                     /old_locus_tag="GUJ28_25435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(2719..3060)
                     /locus_tag="GUJ28_RS25440"
                     /old_locus_tag="GUJ28_25440"
     CDS             complement(2719..3060)
                     /locus_tag="GUJ28_RS25440"
                     /old_locus_tag="GUJ28_25440"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058760.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            3108..3245
                     /locus_tag="GUJ28_RS25445"
                     /old_locus_tag="GUJ28_25445"
     CDS             3108..3245
                     /locus_tag="GUJ28_RS25445"
                     /old_locus_tag="GUJ28_25445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(3444..3602)
                     /locus_tag="GUJ28_RS26290"
     CDS             complement(3444..3602)
                     /locus_tag="GUJ28_RS26290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(4272..4463)
                     /locus_tag="GUJ28_RS25450"
                     /old_locus_tag="GUJ28_25450"
     CDS             complement(4272..4463)
                     /locus_tag="GUJ28_RS25450"
                     /old_locus_tag="GUJ28_25450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4527..4850
                     /locus_tag="GUJ28_RS25455"
                     /old_locus_tag="GUJ28_25455"
     CDS             4527..4850
                     /locus_tag="GUJ28_RS25455"
                     /old_locus_tag="GUJ28_25455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5059..>5490
                     /locus_tag="GUJ28_RS26025"
                     /pseudo=""
     CDS             5059..>5490
                     /locus_tag="GUJ28_RS26025"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5528..6046
                     /locus_tag="GUJ28_RS25465"
                     /old_locus_tag="GUJ28_25465"
     CDS             5528..6046
                     /locus_tag="GUJ28_RS25465"
                     /old_locus_tag="GUJ28_25465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            6053..6286
                     /locus_tag="GUJ28_RS25470"
                     /old_locus_tag="GUJ28_25470"
     CDS             6053..6286
                     /locus_tag="GUJ28_RS25470"
                     /old_locus_tag="GUJ28_25470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          4100..4187
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(3531..4086)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.699"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4841..6390
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgtcagccag cagcctggat tttttcgggg taaggtcttt gctgcggtaa tcagtacccg
       61 caaattgagt tgtgccccgg agtgagttta aaaattcctg gctgtgaaac tgctgaatgc
      121 gttgttgcac atcactccgt gattttcctg tcgctttatc tttagctccc atatcccaga
      181 actgccgaaa aaaaggcacc atcattcgtg ttgtgtcggc tttttgggtg gaaaagttgg
      241 gatcaaaacc gtcatcagaa gcatatacgt aatgccgggt atgttgttcc acgctgccct
      301 ttggggtaca ggctgtgaga gctactgttg ctgttatcat ggcgataaga caaagtttgt
      361 ttccggtacg tctcatgagt gtctccctac cttaaagtat ttattctcgg tgagttatta
      421 tccgaagact attttatcat cagtctctga cagcgttcag cgaccgaaca acctggacca
      481 gaatccccgt ttcttttcca gttccagtgc ctgcctgagc tgggctatct cattttgtag
      541 ctgttcccgt tctgcttcct ggcgtctgcg atccatatcc tgtgcctgtt tatcctcaag
      601 catcagcgtc aggcattgtt tcagctcatt cagctcccgg agaatgcgtt ctgtctgctg
      661 atcatgtgga ttttctgcat gtccctcact gtgcctttct ggtgtctcat tctgctttaa
      721 ttcgccgtat gcccggatca gttcactggt ttcaaactcc cgtcgtccgt ccctgccggt
      781 acggtaactg acaaggcctg cgcgcatatc acggtaaaac tgtgaacgcg atctgcctgt
      841 catttttagt gcgtcccgga gtgtgtgcca tgccataaag tgacagtgtc ccatagatgt
      901 ctcatctcat agtttcagta aaacataatg aggtctgaga acggtaatgt ttgtgctggt
      961 ttttgtggca tcgggcgaga atagcgcgtg gtgtgaaaga ctgttttttt gatcgttttc
     1021 acaaaaatgg aagtccacag tcttgacagg gaaaatgcag cggcgtagct tttatgctgt
     1081 atataaaacc agtggttata tgtacagtat ttatttttaa cttattgttt taaaagtcaa
     1141 agaggatttt ataatggaaa ccgcggtagc gtactataaa gatggtgttc cttatgatga
     1201 taagggacag gtaattatta ctcttttgaa tggtactcct gacgggagtg gctctggcgg
     1261 cggaggtgga aaaggaggca gtaaaagtga aagttctgca gctattcatg caactgctaa
     1321 atggtctact gctcaattaa agaaaacaca ggcagagcag gctgcccggg caaaagctgc
     1381 agcggaagca caggcgaaag caaaggcaaa cagggatgcg ctgactcagc gcctgaagga
     1441 tatcgtgaat gaggctcttc gtcacaatgc ctcacgtacg ccttcagcaa cagagcttgc
     1501 tcatgctaat aatgcagcta tgcaggcgga agcagagcgt ttgcgccttg cgaaagcaga
     1561 agaaaaagcc cgtaaagaag cggaagcagc agaaaaggct tttcaggaag cagaacaacg
     1621 acgtaaagag attgaacggg agaaggctga aacagaacgc cagttgaaac tggctgaagc
     1681 tgaagagaaa cgactggctg cattgagtga agaagctaaa gctgttgaga tcgcccaaaa
     1741 aaaactttct gctgcacaat ctgaagtggt gaaaatggat ggagagatta agactctcaa
     1801 ttctcgttta agctccagta tccatgcccg tgatgcagaa atgaaaacgc tcgctggaaa
     1861 acgaaatgaa ctggctcagg catccgctaa atataaagaa ctggatgagc tggtcaaaaa
     1921 actatcacca agagccaatg atccgcttca gaaccgtcct ttttttgaag caaccagacg
     1981 acgggttggg gccggtaaga ttagagaaga aaaacaaaaa caggtaacag catcagaaac
     2041 acgtattaac cggataaatg ctgatataac tcagatccag aaggctattt ctcaggtcag
     2101 taataatcgt aatgccggta tcgctcgtgt tcatgaagct gaagaaaatt tgaaaaaagc
     2161 acagaataat ctccttaatt cacagattaa ggatgctgtt gatgcaacag ttagctttta
     2221 tcaaacgctg actgaaaaat atggtgaaaa atattcgaaa atggcacagg aacttgctga
     2281 taagtctaaa ggtaagaaaa tcggcaatgt gaatgaagct ctcgctgctt ttgaaaaata
     2341 caaggatgtt ttaaataaga aattcagcaa agccgatcgt gatgctattt ttaatgcgtt
     2401 ggcatcggtg aagtatgatg actgggctaa acatttagat cagtttgcca agtacttgaa
     2461 gattacgggg catgtttctt ttggatatga tgtggtatct gatatcctaa aaattaagga
     2521 tacaggtgac tggaagccac tatttcttac attagagaag aaagctgcag atgcaggggt
     2581 gagttatgtt gttgctttac tttttagctt gcttgctgga actacattag gtatttgggg
     2641 tattgctatt gttacaggaa ttctatgctc ctatattgat aagaataaac ttaatactat
     2701 aaatgaggtg ttagggattt aattattctt tattgatata aaaagtccta gcaatccaaa
     2761 tgggattgct aggaccaaac aaagtagatt atatagcata aataggttta attttgctac
     2821 gggggcgtta tttaggtttt ttcttctttc gaaaaaatct ttctttatga agttaaaagc
     2881 tatgtattca atagcatatt ttgaatatgg acatagaata gtgcttatca ctattgcata
     2941 tagcatctta tctgacacaa ggaaataata cccttcgctg ttttttgtta taaggtatat
     3001 atatataagt gtgcagtaca ggccaaataa aatatttttt atgtagtatc ttaagctcat
     3061 aaattaaacc tcgccatata ttcttttcat tttataagga tcgagttatg aggaaaagat
     3121 tttttgtggg aatattcgcg ataaacctcc ttgttggatg tcaggctaac tatatacgtg
     3181 atgttcaggg agggaccatc gcaccatcct cctcttctaa actgacgggg atcgcggttc
     3241 agtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     3301 gcaaacaaaa aaaccaccgc taccaacggt ggtttgtttg ccggatcaag agctaccaac
     3361 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     3421 gtagccgtag tcgggccact acttcaagaa ctctgtagca ccgtttgtgc catcatcgct
     3481 ctgctaatcc ggttaccagt ggctgctgcc agtggcgtta aggcgtgcct taccgggttg
     3541 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3601 acacagccca gcttggagcg aacgacctac accgaactga gataccaaca gcgtgagcta
     3661 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3721 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3781 cctgtcgggt ttcgccacct ctgacttgag cgtctatttt tgtgatgctc gtcagggggg
     3841 cggagcctat ggaaaaacgc ctgctacgtg gccttcttcc tgttcctggt cttttgctca
     3901 catgttcttt ccggccttat cccctgattc tgtggataac tgtgttaccg tttttgtgtg
     3961 agtcagtacc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc
     4021 ggaaaagcgc ctggacgtgc attttctcct tacgcatctg tgcggcattt cacacccggc
     4081 atggcgtact tttcatacaa tccgcactga tgccgcatgg ttaagccagt atacactccg
     4141 ctatcgctac gtgactgggt cagggctgcg ccccgacacc cgctaaaacc tgctgacgcg
     4201 ccctgacggg cttgtcagct cccggcatcc gctcacagac aagctgtgac cgtctccggg
     4261 agctgcatgt gtcagaggtt ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa
     4321 agctcatcgg cgtggtcgtg aagcgattca caaatatcgg cctgttcatc tgcgtccagt
     4381 tcgttgagct tctccagcag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     4441 gcggtttttt cctgtttagt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     4501 taatgatacc gatgaaacgc gagaggatgc tcacaatacg ggttactgat gatgaacatg
     4561 cccggttact tgaacgctgt gagggtaaac aactggcggt atggatgcgg cgggtctgcc
     4621 tgggggagcc ggttgcccgt tccggaaaac tgccgacact ggcaccgccg ttactgcgtc
     4681 agctggccgc catcggaaat aacctgaatc agacagcccg taaggtgaac agcgggcagt
     4741 ggtcttccgg tgaccgggtt caggtggtgg ccgcactgat ggccatcggg gatgagctgc
     4801 gccggctgcg tctggctgtc agggaacagg gggcgcggga tgatagttaa atttcatgcc
     4861 aggggaaaag gtggtggcag tggtccggtt gattacctgc tggggaggga gcgtaaccgc
     4921 gaaggcgcaa cggtgcttca gggtaatccg gaagaagtcc gggaactcat cgatgccacg
     4981 ccatttgcga agaaatacac gtccggtgtt ctgtcgttcg cggagaagga gctgccgccg
     5041 ggaggacgtg aaaaagtgat ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa
     5101 aatcagtaca gcatcctgtg ggtggagcac caggacaagg gacggcttga gctgaatttt
     5161 gtcattccga acatggagct acagaccgga aaacgcctcc agccgtacta cgaccgcgca
     5221 gacaggccta gaattgatgc ctggcagacg ctggtgaatc accattacgg gctgcatgac
     5281 ccgaacgccc cggagaaccg caggacgctg acactccctg ataacctgcc tgaaacgaaa
     5341 caggcgcttg ctgagggcgt cacgcgaggt atagatgcac tttaccatgc cggagagata
     5401 aaaggccgtc aggatgtgat tcaggcgctc actgaggcgg ggctggaagt ggtcagggtg
     5461 acgcgaagca gtatcagcat tgcagatccg aacggcggga agaatatcag gctgaaagga
     5521 gcattttatg agcaatcttt tgcagacggg cgcggagttc gagaaaaagc tgaaagagag
     5581 agccgaatct acagagaaaa tgctgaacaa cgagttcagg aggctcggcg aatctgtaag
     5641 cgaggctgtg acatcaaacg agacgaaaat cagagacgct atagccctgt tcacagcctc
     5701 gacagaggaa tcgctggaaa aacaccggga aggggtgaaa gaggcgatga tgcagcacag
     5761 gagggacgtg ttaaagctgg cagggaatac gggcatgatg ttactgggga tagtctttct
     5821 cctgtttacc gcgagtggcg ggacgctctg gtatcttgga gggaggatac aggcgaacct
     5881 ggaagaaatc aggaagcagg aagagacatt gcagaaactg aacgcgaaga catggggcgt
     5941 ggagtttgtg caggacggga acaggaaatt ccttgtcctt ccgtacggga aatcagcgga
     6001 ggtgattccc tttcagggga aagagtgggt acatctgaag gagtgacaca gagtgacaga
     6061 gctggaaaca catttgctga acgccttaga gcagctgcaa caggactata tgcagcggct
     6121 gagcgaatgg gagagcgcct tcgtggaatt gcagaagatg ttttcgctta cgcaacggga
     6181 caacgcgatg ctgaacgagc gggtcatgca gttgagtcag caggtgcagc acttgagcga
     6241 gcagacagaa cgcttgagcc agttatacag cgagaactgg agataagaga ggaacggctg
     6301 atacaggagc gcgaacatgt gttatccctg gaacgggagc gtcagccgga aatacaggaa
     6361 cgcacgctgg atggcccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg
     6421 attaagtggg ggtaatgtgg cctgtaccct ctggttgcat aggtattcat acggttaaaa
     6481 tttatcaggc gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc
     6541 tgccgtgatc gcgctgaacg cgttttagcg gtgcgtacaa ttaagggatt atggtaaatc
     6601 cacttactgt ctgccctcgt agccatcgag ataaaccgca gaaatcg
//
