LOCUS       NZ_CP047474             9578 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Escherichia coli strain DY612 plasmid pDY612-5, complete sequence.
ACCESSION   NZ_CP047474
VERSION     NZ_CP047474.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13742266
            Assembly: GCF_029636765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Lai,Y., Tang,Y. and Wang,H.
  TITLE     Colocation of tet(X4) and cfr in E. coli of swine origin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9578)
  AUTHORS   Lai,Y., Tang,Y. and Wang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2020) College of Life Science, Sichuan University,
            No.29 Wangjiang Road, Chengdu, Sichuan 610064, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. V4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 286.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029636765.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:36:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,635
            Genes (coding)                    :: 4,392
            CDSs (with protein)               :: 4,392
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 60 of 243
            Pseudo Genes (incomplete)         :: 165 of 243
            Pseudo Genes (internal stop)      :: 49 of 243
            Pseudo Genes (multiple problems)  :: 28 of 243
            Pseudo Genes (short protein)      :: 1 of 243
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047474.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="DY612"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="pDY612-5"
                     /geo_loc_name="China"
                     /lat_lon="30.67 N 104.06 E"
                     /collection_date="2019-03-03"
                     /collected_by="Yan Lai"
     gene            complement(join(9148..9578,1..685))
                     /gene="mobA"
                     /locus_tag="GTK51_RS23730"
                     /old_locus_tag="GTK51_23450"
     CDS             complement(join(9148..9578,1..685))
                     /gene="mobA"
                     /locus_tag="GTK51_RS23730"
                     /old_locus_tag="GTK51_23450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobA"
                     /protein_id="WP_016946648.1"
                     /translation="MASYHLSVKTGGKGSASPHADYIAREGKYAREKDSDLEHKESGNM
                     PAWAAHKPSEFWKAADTSERANGCTYREIEIALPRELKPEQRLELVRDFVQQEIGDRHA
                     YQFAIHNPKAAIAGGEQPHAHIMFSERINDGIHRDPEQYFKRANTKEPERGGAKKARFG
                     ETPTERKEYLVAQRERWADLQNKHLERYQHADRVDSRSLKAQGIDREPERHLGAGQVQR
                     FDTEQLQAILERREAERQVQQSRDERDSVIDVTTSLREALSERDTLTLKQELKSEPEQE
                     SHSGRTFDFEKEPDKLNALVSDAMKDIQEEIDLQSLVNDAMAEFQGIHQEMERQRERER
                     LAEKQRQQEKERQRLAEQIRQKPDKGWSFSR"
                     /name="mobA"
     gene            849..1316
                     /gene="traD"
                     /locus_tag="GTK51_RS23735"
                     /old_locus_tag="GTK51_23455"
     CDS             849..1316
                     /gene="traD"
                     /locus_tag="GTK51_RS23735"
                     /old_locus_tag="GTK51_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_016946647.1"
                     /translation="MNKSEKQEQWAAERVQYIRGLKSPNEQQKLMLILTDKADKTAQDI
                     KTLSLLMKAEQAAEKAQEARAKVMNLIQAEKRAEARAARKARDHALYQSAGLLILAGLV
                     DSKTGKPVDDTAALLGALASLNDLSRDNPKWSDWKIRGQELLNSKKSDSTT"
                     /name="traD"
     gene            1910..2860
                     /locus_tag="GTK51_RS23740"
                     /old_locus_tag="GTK51_23460"
     CDS             1910..2860
                     /locus_tag="GTK51_RS23740"
                     /old_locus_tag="GTK51_23460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016946646.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001293213.1"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
                     /name="replication initiation protein"
     gene            2868..3281
                     /locus_tag="GTK51_RS23745"
                     /old_locus_tag="GTK51_23465"
     CDS             2868..3281
                     /locus_tag="GTK51_RS23745"
                     /old_locus_tag="GTK51_23465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012443152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001229901.1"
                     /translation="MALVSISEASRLTNKSRTTVHRYISKGKLSICTDENGVKKIDTSE
                     LLRVFGAFKAVHHEQIDNVTDEQHVTPNRTGETSKTKQLEHEIEHLRQLVTAQQSHIDS
                     LKQAMLLIESKLPTTREPVTPPVGKKSWQFWKK"
                     /name="hypothetical protein"
     gene            complement(3516..8438)
                     /locus_tag="GTK51_RS23750"
                     /old_locus_tag="GTK51_23470"
     CDS             complement(3516..8438)
                     /locus_tag="GTK51_RS23750"
                     /old_locus_tag="GTK51_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005204747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type ISP restriction/modification enzyme"
                     /protein_id="WP_274413140.1"
                     /translation="MNALSALLTKIEQASPTQRDKGTTFENLCVQYFLHEPKYAELYSD
                     VLSYGGWVSQYGETVGITKKKDDGIDLVAVTKTGEFHAIQCKNYNQTKIAKKDIDSFLA
                     ASDKTYFTLRYIVASTDNWTEEAKNMLRDKAVPVTALSLTDLEQSALDWSQFDFDPAYK
                     PVMKAKKQLRPHQTPALEAVKRGLATADRGKLIMACGTGKTFTSLRIAEAVAGRGKTVL
                     FLVPSLALLSQTLDEWTQDTLIDLRCFAVCSDSDVGKKNHDDNVVVGISDLKYPATTNA
                     SSLVKAFNQPDIFGSDKPPYMNVVFSTYHSVEVIHQAQKLGFPAFDFIICDEAHRTTGA
                     TFEGDDESAFVRIHDNAYIAGQKRLYMTATPRIFGDDAKETEGVTLCSMDDKSLYGDDL
                     YVITFSKAVQLGILCDYKVIVLAVEEKHVSRRIQSLLKDENNQLKVDDAAKIVGCWKAL
                     AKQGLAGADGVHPDAMKRAVAFCQVIEKDFRGKTHKVSSKLITEMFGAVVEAYQASEIE
                     LLRETAPDKAIDPSLKLQCQVKHVDGSMNATEKKSKIEWLKADTDENVCRILSNVRCLS
                     EGVDVPSLDAVLFLTPRSSQVDVVQSVGRVMRRAEGKELGYVILPVVIPAGEEPENALN
                     NNQAYRVVWQVLNALRAHDDRFDAMINKLEFNGKDTSRMEVIAVADKVVKKTKRQTKKS
                     ELAGKARKSSGIGSAVSPVPEQFDIEFSVGEIERALYAKIVKKCGNRHHWEDWANDIAK
                     IARTHIDRIHGILENPGNQVEISAFRAFADELRDDLNNSVSDDEIIEMLAQHLITKPVF
                     DALFADYNFTDHNPMSMAMQNVLNTLQEHHLEKETSTLNSFYESVKMRADGITTAEGKQ
                     QIIVQLYDKFFRNAFPRMTERLGIVYTPVEVVDFIIHSVNDVLKQEFGQTLADKGVHIL
                     DPFTGTGTFITRLLQSGLIPSDKLAYKFKNEIHANEIVLLAYYIAAINIEAVYHGITEE
                     SEYTPFEGICLTDTFEMYEKDDLVSEVLVDNSERRKRQKALDIRVIIGNPPYSSGQESA
                     NDNNQNVKYPMLDKRIAETYAACTTATNKNALYDSYIRAIRWASDRIGEQGVIGFVTNG
                     GYIDSNSADGLRKCLADEFSSLYFFHLRGNARTSGEVRRKEKDNVFGQGTRTPIVIAIL
                     VKNPAAIQSGQIYFHDIGDYLTREEKLEKISEFSSINGIEKSHGWQSITPDTYNDWLNQ
                     RDDSFYDFIQIGNKKDKENSSLFDVYSTGLQPRGDAWFYNYSKQKLAMNIKKSINFFNS
                     EVDRYKKEYNSGSLKDFVRIDPFNFAWYGESYKEISRGRYYDIADGVVRVSLYRPYSKS
                     LVYFNENMMHRVSKIPEIFPEKNSNNLIIIITGRGSTKGFSSLISNEIVDLECISKGQC
                     FPLYVYDNKKPSILFGNQDESVYLDGEYERKYAISDTGMIYFKKHYPEEKINQEDVFYY
                     IYGLLHSEDYRSRYADNLSKELPYIPCVKKIEDFWSFSKAGRELAELHLNYETVEPYKA
                     DIDTGSLSYSQLGKDDFYVEKMKFAKKGEKNTVIYNNKIRIKNIPIEAYDYIVNGKPAL
                     EWVMERQGVSTHKDSGIVNDANDWAIEIMDNPRYPLELFLRVITVSLETQKIVNSMPKL
                     DI"
                     /name="type ISP restriction/modification enzyme"
     gene            complement(8551..9135)
                     /locus_tag="GTK51_RS23755"
                     /old_locus_tag="GTK51_23475"
     CDS             complement(8551..9135)
                     /locus_tag="GTK51_RS23755"
                     /old_locus_tag="GTK51_23475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein MobX"
                     /protein_id="WP_010891053.1"
                     /translation="MSDEIKQIAALIGHHQALEKRVTSLTEQFQAASSQLQQQSETLSR
                     VIRELDSASGNMTDTVRKSVSSALTQVEKELKQAGLAQQKPATEALNQAADTAKAMIHE
                     MRREMSRYTWKSAIYLVLTIFFVLASCVTAFTWFMNDGYSQIAEMQRMEAVWQKKAPLA
                     DISRCDGKPCVKVDTRTTYGDKENTWMIIKK"
                     /name="mobilization protein MobX"
     Typing          2685..2860
                     /name="ColE10"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(3..685)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.86"
                     /coverage="99.0"
                     /evalue="3.48e-142"
     Typing          complement(9152..9576)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="7.68e-61"
ORIGIN
        1 gtagctgctc agtatcgaat cgctgaacct gacccgcgcc cagatgccgc tcaggctcac
       61 ggtcaatgcc ctgcgccttc agggaacggg aatcaacccg gtcagcgtgc tgataccgtt
      121 caaggtgctt attctggagg tcagcccagc gttccctctg ggcaacaagg tattctttgc
      181 gttcggtcgg tgtttccccg aaacgtgcct tttttgcgcc accgcgctcc ggctctttgg
      241 tgttagcccg tttaaaatac tgctcagggt cacggtgaat accgtcatta atgcgttcag
      301 agaacatgat atgggcgtgg ggctgctcgc caccggctat cgctgctttc ggattatgga
      361 tagcgaactg ataggcatgg cggtcgccaa tttcctgttg gacaaaatcg cggacaagct
      421 caagacgttg ttcgggtttt aactcacgcg gcagggcaat ctcgatttca cggtaggtac
      481 agccgttggc acgttcagac gtgtcagcgg ctttccagaa ctcggacggt ttatgcgctg
      541 cccacgccgg catattgccg gactccttgt gctcaaggtc ggagtctttt tcacgggcat
      601 actttccctc acgcgcaata taatcggcat gaggagaggc actgcctttt ccgccggttt
      661 ttacgctgag atgataggat gccatcgtgt tttatcccgc tgaaggcgcg caccgtttct
      721 gaacgaagtg aagaaacgtc taagtgcgcc ctgataaata aaagagttat cagggattgt
      781 agtgggattt gacctcctct gccatcactg agcataatca ttccgttagc attcaggagg
      841 taaacagcat gaataaaagc gaaaaacagg aacaatgggc agcagaaaga gtgcagtata
      901 ttcgcggctt aaagtcgccg aatgagcaac agaaacttat gctgatactg acggataaag
      961 cagataaaac agcacaggat atcaaaacgc tgtccctgct gatgaaggct gaacaggcag
     1021 cagagaaagc gcaggaagcc agagcgaaag tcatgaacct gatacaggca gaaaagcgag
     1081 ccgaagccag agccgcccgt aaagcccgtg accatgctct gtaccagtct gccggattgc
     1141 ttatcctggc gggtctggtt gacagtaaga cgggtaagcc tgttgatgat accgctgcct
     1201 tactgggtgc attagccagt ctgaatgacc tgtcacggga taatccgaag tggtcagact
     1261 ggaaaatcag agggcaggaa ctgctgaaca gcaaaaagtc agatagcacc acatagcaga
     1321 cccgccataa aacgccctga gaagcccgtg acgggctttt cttgtattat gggtagtttc
     1381 cttgcatgaa tccataaaag gcgcctgtag tgccatttac ccccattccc tgccagagcc
     1441 gtgagcgcag cgaactgaat gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga
     1501 gacaaaagga atattcagcg atttgcccga gcttgcgagg gtgctactta agcctttagg
     1561 gttttaaggt ctgttttgta gaggagcaaa cagcgtttgc gacatccttt tgtaatactg
     1621 cggaactgac taaagtagtg agttatacac agggctggga tctattcttt ttatcttttt
     1681 ttattctttc tttattctat aaattataac cacttgaata taaacaaaaa aaacacacaa
     1741 aggtctagcg gaatttacag agggtctagc agaatttaca agttttccag caaaggtcta
     1801 gcagaattta cagataccca caactcaaag gaaaaggact agtaattatc attgactagc
     1861 ccatctcaat tggtatagtg attaaaatca cctagaccaa ttgagatgta tgtctgaatt
     1921 agttgttttc aaagcaaatg aactagcgat tagtcgctat gacttaacgg agcatgaaac
     1981 caagctaatt ttatgctgtg tggcactact caaccccacg attgaaaacc ctacaaggaa
     2041 agaacggacg gtatcgttca cttataacca atacgctcag atgatgaaca tcagtaggga
     2101 aaatgcttat ggtgtattag ctaaagcaac cagagagctg atgacgagaa ctgtggaaat
     2161 caggaatcct ttggttaaag gctttgagat tttccagtgg acaaactatg ccaagttctc
     2221 aagcgaaaaa ttagaattag tttttagtga agagatattg ccttatcttt tccagttaaa
     2281 aaaattcata aaatataatc tggaacatgt taagtctttt gaaaacaaat actccatgag
     2341 gatttatgag tggttattaa aagaactaac acaaaagaaa actcacaagg caaatataga
     2401 gattagcctt gatgaattta agttcatgtt aatgcttgaa aataactacc atgagtttaa
     2461 aaggcttaac caatgggttt tgaaaccaat aagtaaagat ttaaacactt acagcaatat
     2521 gaaattggtg gttgataagc gaggccgccc gactgatacg ttgattttcc aagttgaact
     2581 agatagacaa atggatctcg taaccgaact tgagaacaac cagataaaaa tgaatggtga
     2641 caaaatacca acaaccatta catcagattc ctacctacat aacggactaa gaaaaacact
     2701 acacgatgct ttaactgcaa aaattcagct caccagtttt gaggcaaaat ttttgagtga
     2761 catgcaaagt aagtatgatc tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac
     2821 cacactagag aacatactgg ctaaatacgg aaggatctga ggttcttatg gctcttgtat
     2881 ctatcagtga agcatcaaga ctaacaaaca aaagtagaac aactgttcac cgttacatat
     2941 caaagggaaa actgtccata tgcacagatg aaaacggtgt aaaaaagata gatacatcag
     3001 agcttttacg agtttttggt gcattcaaag ctgttcacca tgaacagatc gacaatgtaa
     3061 cagatgaaca gcatgtaaca cctaatagaa caggtgaaac cagtaaaaca aagcaactag
     3121 aacatgaaat tgaacacctg agacaacttg ttacagctca acagtcacac atagacagcc
     3181 tgaaacaggc gatgctgctt atcgaatcaa agctgccgac aacacgggag ccagtgacgc
     3241 ctcccgtggg gaaaaaatca tggcaattct ggaagaaata gcgctttcag ccggcaaacc
     3301 ggctgaagcc ggatctgcga ttctgataac aaactagcaa caccagaaca gcccgtttgc
     3361 gggcagcaaa acccgtactt ttggacgttc cggcggtttt ttgtggcgag tggtgttcgg
     3421 gcggtgcgcg caagatccat tatgttaaaa acgacaggat ttatgaaaat aaccagaatt
     3481 gtgcaggatg ttttttcatc ctgcacacct agtgattaga tatctagttt tggcatgcta
     3541 ttaacgattt tctgagtttc aagactgact gtgataactc tcaggaaaag ctctagtggg
     3601 tatctagggt tatccataat ttcaattgcc cagtcattag catcgttcac aatcccgcta
     3661 tctttatggg tagaaactcc ctgccgctcc atcacccact cgagagcagg cttgccattc
     3721 acaatgtagt cataggcttc aatagggatg ttttttatgc gaattttatt gttataaata
     3781 acggtatttt tttcaccttt tttggcgaac ttcatcttct caacataaaa atcgtcttta
     3841 cctaactgag aataactcag ggatcccgta tcaatatcag ccttgtatgg ctcaactgtt
     3901 tcgtagttta aatgcaactc agcaagttca cggcctgctt tagaaaacga ccaaaaatcc
     3961 tcaatttttt tcacacatgg tatatatggc aactccttac tcaaattatc cgcatagcga
     4021 gagcgataat cttcactgtg caacaaacca taaatgtaat aaaaaacatc ctcctgattt
     4081 attttttctt ccggataatg ctttttaaaa taaatcatcc ctgtatcact aatggcatat
     4141 tttcgctcat actcaccatc taaatacaca ctttcatctt gattgccaaa caaaatacta
     4201 ggttttttat tatcgtaaac atagagaggg aagcattgcc ccttagatat acactcaaga
     4261 tcaacaattt catttgatat taatgaagag aaccctttag ttgagccacg ccctgtaata
     4321 atgataatta aattattaga gtttttctct gggaaaattt caggaatttt gctaacacga
     4381 tgcatcatgt tctcattaaa gtaaaccaaa gacttactat atggtctata taaggaaact
     4441 ctaacaacac catcagcaat atcataatat ctacctctgg agatttcttt gtaactttcc
     4501 ccataccaag caaaattaaa tgggtcaatt cttacaaagt ccttcaagct ccctgaatta
     4561 tattcttttt tataccgatc aacttcagaa ttaaaaaaat taatggactt ttttatgttc
     4621 atggctaatt tttgttttga gtagttataa aaccatgcgt cacctctagg ctgaagccct
     4681 gttgaataaa catcgaaaag agatgagttt tctttatctt ttttattgcc tatttggata
     4741 aagtcataaa aactgtcatc acgctgattc agccagtcat tgtaggtatc aggtgtaatt
     4801 gactgccaac catgtgattt ttcaatgcca ttaatacttg agaattcgga aattttctcc
     4861 agtttttctt cacgagtcag gtagtcacca atatcatgaa aataaatctg accggactgt
     4921 attgcagccg gattttttac aagaatagca atcacaatcg gcgttcttgt tccctgacca
     4981 aaaacgttat ctttttcttt tcgacgaacc tctcctgacg ttctagcatt tcctctcaaa
     5041 tggaaaaaat acagactact gaactcatca gccagacact tacgcagacc atcagccgag
     5101 ttagagtcga tatagccacc attagtcaca aagccgatta caccctgctc accgatacgg
     5161 tcactcgccc agcgaattgc ccgaatatag gagtcataga gtgcattctt gttggtcgca
     5221 gtggtacaag cagcatacgt ctctgcaatc cgcttatcga gcatcggata ttttacattc
     5281 tggttattat cgtttgcaga ttcctgaccg gatgaataag gcggattacc aataattact
     5341 cgaatatcca gagccttctg ccgtttacga cgttcactgt tatcgaccaa aacctcactg
     5401 accagatcat ccttctcata catttcaaac gtatctgtca gacaaatccc ctcaaacggc
     5461 gtgtattctg attcttccgt gatgccgtga tacaccgcct cgatattaat ggcggcaatg
     5521 taataggcta acaggacgat ttcgttggca tggatttcat ttttaaactt gtaagccagt
     5581 ttatctgacg ggataagacc actttgcagc aggcgggtga tgaacgtacc ggttccggtg
     5641 aacgggtcaa gaatatgcac acctttgtcg gcgagtgttt gtccgaactc ctgtttcagc
     5701 acatcattca cgctgtgaat aataaaatca acgacttcaa caggcgtata cacaattccc
     5761 agacgctccg tcattctcgg gaacgcattt ctgaagaact tatcgtacaa ctgtacgata
     5821 atctgctgct taccttcagc ggtggtaatg ccgtccgcac gcatttttac cgactcgtag
     5881 aagctgttca gcgtgctggt ttctttttcg agatgatgct cctgcaacgt gttaagcacg
     5941 ttctgcatcg ccatgctcat cgggttatgg tcggtaaaat tgtaatcggc aaacagggca
     6001 tcaaagaccg gcttggttat cagatgctgt gccagcattt caatgatttc atcgtcggaa
     6061 acactgttat tcagatcatc acgcagttca tcggcaaatg ccctgaaggc actgatttct
     6121 acttgattac ccggattttc gagaatgccg tgaatgcggt cgatatgcgt gcgggcaatt
     6181 ttagcgatat cgttcgccca gtcttcccag tgatgccggt taccgcattt tttaacgatt
     6241 ttcgcgtaca gggcacgctc aatctcaccg actgaaaact caatatcaaa ctgttcagga
     6301 acgggactca cggctgaacc aatgccactg cttttacgcg cttttcctgc cagttcagat
     6361 tttttcgtct gccgtttagt tttcttcacc actttatcgg ctaccgcgat cacttccatt
     6421 ctgctggtat ctttaccgtt aaactccagc ttgttgatca tggcatcaaa gcggtcatca
     6481 tgcgccctga gtgcattcag cacctgccag acaacacggt aggcctgatt gttgtttaag
     6541 gcgttttccg gctcttctcc ggcgggaatg acaaccggca gaatgacata gcccagctct
     6601 ttaccttcgg cacgccgcat aacacgcccg acagactgca ccacatccac ctgagagctt
     6661 ctcggcgtta aaaacaacac cgcatccaga gaaggcacat ccacgccttc agataaacaa
     6721 cggacgttag acaatatccg gcagacattt tcatccgtgt ccgctttcag ccattcaatt
     6781 ttggattttt tctcagtggc attcatgctg ccgtcaacgt gcttcacctg acactgaagt
     6841 tttaatgatg ggtcaatcgc cttatccggt gcggtttccc tgagaagttc gatctcggat
     6901 gcctgataag cctcaaccac tgctccgaac atttccgtga tcagtttaga actgacctta
     6961 tgcgtctttc cgcgaaagtc tttttctatc acctgacaga acgcaacagc ccgtttcatc
     7021 gcatcagggt gaacgccatc tgcccccgcc agtccctgct ttgccagtgc tttccagcaa
     7081 cccacaatct ttgccgcatc atcgactttg agctgattat tttcatcctt cagcagagac
     7141 tgaatgcggc ggctgacatg cttttcttcc acggcaagca caataacttt gtaatcacac
     7201 aaaatgccca actggacggc tttagagaag gtaatgacat acaaatcatc cccataaaga
     7261 cttttgtcat ccattgagca tagggtaact ccctcagtct ctttcgcatc atctccaaaa
     7321 atgcgcggag tcgcggtcat gtacaggcgt ttttgaccgg cgatataggc gttgtcgtga
     7381 atacgaacaa aggcggactc atcatcccct tcaaaggtcg cacctgtggt ccggtgagct
     7441 tcatcacaaa tgatgaaatc aaatgcagga aaacccagtt tttgcgcctg atggatgacc
     7501 tcaacagagt ggtaggtaga aaacaccacg ttcatataag gcggcttatc actgccgaaa
     7561 atatccggct gattaaacgc tttgacgagg ctgctagcat tcgtggtcgc cggatatttc
     7621 aggtcgctga taccgacaac aacattatca tcatggtttt tcttacccac atcactgtca
     7681 gagcagaccg caaaacatct caggtctatc agcgtatcct gcgtccattc atccagcgtc
     7741 tgactcaaca gtgccagact tggcacaagg aacagtacgg ttttacctct tccggcaacg
     7801 gcttcagcaa tacgcagaga ggtgaaggtt tttcctgtgc cgcacgccat aatgagcttg
     7861 ccccgatcgg cagtcgccag ccctcgttta acggcttcaa gcgcgggagt ctgatgaggc
     7921 cggagttgct ttttggcttt catcaccggc ttgtaggccg gatcaaaatc gaattgtgac
     7981 cagtccagtg cgctttgctc aaggtctgtc aggcttaacg cagtgacagg aacggccttg
     8041 tcccgcagca tatttttagc ttcctccgtc cagttatccg tagaggcaac gatatagcgg
     8101 agcgtaaaat acgttttatc ggaggcagcc agaaaactat caatatcctt tttggcgatt
     8161 ttagtctgat tgtagttttt gcactggata gcatgaaact cccccgtttt ggttacggcg
     8221 accaaatcaa tgccatcatc ttttttcttc gtgatcccaa cggtttcacc gtactgagaa
     8281 acccatccgc catagctcag cacgtcactg tacagctcgg catatttagg ttcatggagg
     8341 aaatactgca cacaaaggtt ttcaaaggtt gtacctttgt cccgctgtgt tggtgaagcc
     8401 tgttcaattt ttgtcagcaa tgcagaaagt gcattcatcg ttgatatcct taatgtcctt
     8461 tatttgctgg ggttatcaga tccccccgac acgtttaatt aatgctttct ccgccggaga
     8521 tcgacgcaca gcgttctgtg ctctatgatg ttatttctta ataatcatcc aggtattctc
     8581 tttatcacca tacgtagtgc gagtgtccac cttaacgcag ggctttccgt cacagcgcga
     8641 tatgtcagcc agcggggctt tcttttgcca gaccgcttcc atcctctgca tttcagcaat
     8701 ctggctatac ccgtcattca taaaccacgt aaatgccgtc acgcaggaag ccaggacgaa
     8761 gaatatcgtc agtacaagat aaatcgcgga tttccacgta tagcgtgaca tctcacgacg
     8821 catttcatgg atcatcgctt tcgccgtatc ggcagcctga ttcagcgctt ctgtcgccgg
     8881 tttctgctgt gctaatccgg cttgtttcag ttctttctca acctgagtga gcgcggaact
     8941 caccgatttc ctgacggtgt cagtcatatt accggacgcg ctgtccagct cacgaatgac
     9001 cctgctcagc gtttcacttt gctgctgtaa ttgtgatgag gcggcctgaa actgttctgt
     9061 cagagaagta acacgctttt ccagcgcctg atgatgcccg ataagggcgg caatttgttt
     9121 aatttcgtcg ctcatacaaa atcctgccta tcgtgagaat gaccagcctt tatccggctt
     9181 ctgtcgtatc tgttcggcga gtcgctgtcg ttctttctcc tgctgacgct gtttttccgc
     9241 cagacgttcg cgctctctct gcctttccat ctcctgatgt atcccctgga actccgccat
     9301 cgcatcgtta acaagggact gaagatcgat ttcttcctgt atatccttca tggcatcact
     9361 gaccagtgcg ttcagcttgt caggctcttt ttcaaaatca aacgttctgc cggaatggga
     9421 ttcctgctca ggctctgact tcagctcctg ttttagcgtc agagtatccc tctcgctgag
     9481 ggcttcccgt aacgaggtag tcacgtcaat tacgctgtca cgttcatcac gggactgctg
     9541 cacctgcctt tcagcctccc tgcgctcaag aatggcct
//
