LOCUS       NZ_CP047472            12887 bp    DNA     circular CON 27-MAY-2024
DEFINITION  Escherichia coli strain DY612 plasmid pDY612-3, complete sequence.
ACCESSION   NZ_CP047472
VERSION     NZ_CP047472.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13742266
            Assembly: GCF_029636765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12887)
  AUTHORS   Lai,Y., Tang,Y. and Wang,H.
  TITLE     Colocation of tet(X4) and cfr in E. coli of swine origin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12887)
  AUTHORS   Lai,Y., Tang,Y. and Wang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2020) College of Life Science, Sichuan University,
            No.29 Wangjiang Road, Chengdu, Sichuan 610064, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. V4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 286.0x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_029636765.1-RS_2024_05_27
            Annotation Date                   :: 05/27/2024 05:36:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,758
            CDSs (total)                      :: 4,635
            Genes (coding)                    :: 4,392
            CDSs (with protein)               :: 4,392
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 243
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 243
            Pseudo Genes (frameshifted)       :: 60 of 243
            Pseudo Genes (incomplete)         :: 165 of 243
            Pseudo Genes (internal stop)      :: 49 of 243
            Pseudo Genes (multiple problems)  :: 28 of 243
            Pseudo Genes (short protein)      :: 1 of 243
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047472.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="DY612"
                     /host="pig"
                     /db_xref="taxon:562"
                     /plasmid="pDY612-3"
                     /geo_loc_name="China"
                     /lat_lon="30.67 N 104.06 E"
                     /collection_date="2019-03-03"
                     /collected_by="Yan Lai"
     gene            complement(join(12122..12887,1..86))
                     /gene="repC"
                     /locus_tag="GTK51_RS23090"
                     /old_locus_tag="GTK51_22805"
     CDS             complement(join(12122..12887,1..86))
                     /gene="repC"
                     /locus_tag="GTK51_RS23090"
                     /old_locus_tag="GTK51_22805"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            complement(73..912)
                     /locus_tag="GTK51_RS23095"
                     /old_locus_tag="GTK51_22810"
     CDS             complement(73..912)
                     /locus_tag="GTK51_RS23095"
                     /old_locus_tag="GTK51_22810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_094337857.1"
                     /translation="MATHKPINILEAFTAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVLYLPAEDPPTAIHHRLHALGAYLNDEQRQAVAA
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            complement(941..1147)
                     /gene="urfF"
                     /locus_tag="GTK51_RS23100"
                     /old_locus_tag="GTK51_22815"
     CDS             complement(941..1147)
                     /gene="urfF"
                     /locus_tag="GTK51_RS23100"
                     /old_locus_tag="GTK51_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_094336940.1"
                     /translation="MKDHRDTQTGNLLATPNAIRQARFADAQRKLGRKARKIWATDDEA
                     EALRLYLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            complement(1203..2174)
                     /locus_tag="GTK51_RS23105"
     CDS             complement(1203..2174)
                     /locus_tag="GTK51_RS23105"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_236766887.1"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSASEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSELDLEDMKAEGREPALIVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRTGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRYGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELAQAPAPRQRGMDRGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            complement(2171..2584)
                     /locus_tag="GTK51_RS23110"
     CDS             complement(2171..2584)
                     /locus_tag="GTK51_RS23110"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_223384840.1"
                     /translation="MSAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRETQAAWAKLAKELREVASEVSDAAQSARSAARGWHWKLWLTVMAASM
                     MPTLVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            complement(<2739..3332)
                     /locus_tag="GTK51_RS23115"
                     /pseudo=""
     CDS             complement(<2739..3332)
                     /locus_tag="GTK51_RS23115"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            3532..3816
                     /locus_tag="GTK51_RS23120"
                     /old_locus_tag="GTK51_22825"
     CDS             3532..3816
                     /locus_tag="GTK51_RS23120"
                     /old_locus_tag="GTK51_22825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_278182357.1"
                     /translation="MAKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGTMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPA"
                     /name="mobilization protein"
     gene            3843..4010
                     /locus_tag="GTK51_RS23125"
                     /old_locus_tag="GTK51_22830"
     CDS             3843..4010
                     /locus_tag="GTK51_RS23125"
                     /old_locus_tag="GTK51_22830"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_161403145.1"
                     /translation="MTDLKLDDYTDADFIDELLSDELRCECGEELHYYDDGSLSRCNSC
                     DPDRETNPNL"
                     /name="hypothetical protein"
     gene            4654..6147
                     /locus_tag="GTK51_RS23130"
                     /old_locus_tag="GTK51_22835"
     CDS             4654..6147
                     /locus_tag="GTK51_RS23130"
                     /old_locus_tag="GTK51_22835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91-like element ISVsa3 family transposase"
                     /protein_id="WP_001120888.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALV
                     TPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIED
                     PIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91-like element ISVsa3 family transposase"
     gene            complement(<6383..6625)
                     /gene="glmM"
                     /locus_tag="GTK51_RS23135"
                     /old_locus_tag="GTK51_22845"
                     /pseudo=""
     CDS             complement(<6383..6625)
                     /gene="glmM"
                     /locus_tag="GTK51_RS23135"
                     /old_locus_tag="GTK51_22845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008891390.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /name="glmM"
     gene            complement(6712..>6819)
                     /locus_tag="GTK51_RS23140"
                     /old_locus_tag="GTK51_22850"
                     /pseudo=""
     CDS             complement(6712..>6819)
                     /locus_tag="GTK51_RS23140"
                     /old_locus_tag="GTK51_22850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /name="sulfonamide-resistant dihydropteroate synthase Sul2"
     gene            7013..7858
                     /gene="estT"
                     /locus_tag="GTK51_RS23145"
                     /old_locus_tag="GTK51_22855"
     CDS             7013..7858
                     /gene="estT"
                     /locus_tag="GTK51_RS23145"
                     /old_locus_tag="GTK51_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014053561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide hydrolase EstT"
                     /protein_id="WP_164538741.1"
                     /translation="MKEKIIKTNGIELCTESFGNKKNPAILLVAGATVSMLYWDTEFCQ
                     QLSEKGFFVIRYDNRDVGKSTNYEPGSAPYDIVDLTNDAIAILDGYEIDKAHFVGMSLG
                     GLITQIAAIKFADRVSSLTLMSTGPWGDSDPTIPEMDTRILDFHGKAGTVDWSNEDSVV
                     NYLIQGAELMCGKKQFDKQRSEKLIRAEFNRANNYISMFNHATLQGSEEYWNRLNEVNQ
                     PTLIIHGTDDKIWHYKNAGVLIEKIQNTKLITLEGTGHELHFEDWTTIIDGIEKHINEQ
                     "
                     /name="estT"
     gene            7848..9005
                     /locus_tag="GTK51_RS23150"
                     /old_locus_tag="GTK51_22860"
     CDS             7848..9005
                     /locus_tag="GTK51_RS23150"
                     /old_locus_tag="GTK51_22860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014615010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline-inactivating monooxygenase Tet(X4)"
                     /protein_id="WP_094309310.1"
                     /translation="MSNKEKQMNLLSDKNVAIIGGGPVGLTMAKLLQQNGIDVSVYERD
                     NDREARIFGGTLDLHKGSGQEAMKKAGLLQTYYDLALPMGVNIADEKGNILSTKNVKPE
                     NRFDNPEINRNDLRAILLNSLENDTVIWDRKLVMLEPGKKKWTLTFENKPSETADLVII
                     ANGGMSKVRKFVTDTEVEETGTFNIQADIHHPEVNCPGFFQLCNGNRLMAAHQGNLLFA
                     NPNNNGALHFGISFKTPDEWKNQTQVDFQNRNSVVDFLLKEFSDWDERYKELIRVTSSF
                     VGLATRIFPLGKSWKSKRPLPITMIGDAAHLMPPFAGQGVNSGLMDALILSDNLTNGKF
                     NSIEEAIENYEQQMFIYGKEAQEESTQNEIEMFKPDFTFQQLLNV"
                     /name="tetracycline-inactivating monooxygenase Tet(X4)"
     gene            9344..>9505
                     /locus_tag="GTK51_RS23155"
                     /old_locus_tag="GTK51_22865"
                     /pseudo=""
     CDS             9344..>9505
                     /locus_tag="GTK51_RS23155"
                     /old_locus_tag="GTK51_22865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(9610..9987)
                     /locus_tag="GTK51_RS23160"
                     /old_locus_tag="GTK51_22870"
     CDS             complement(9610..9987)
                     /locus_tag="GTK51_RS23160"
                     /old_locus_tag="GTK51_22870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821152.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000203935.1"
                     /translation="MTSRKTTSSAIAHCSTSSHGATTDVLALSQSDVAVLAETFRLLGD
                     QSRLKILLQCMRGSVAVGDIADSLDLSQSLVSHHLRLLRGARLVRGERQAKHIFYGIAD
                     QHVSQVLQDMAVHISEDRNDD"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(10031..11053)
                     /locus_tag="GTK51_RS23165"
                     /old_locus_tag="GTK51_22875"
     CDS             complement(10031..11053)
                     /locus_tag="GTK51_RS23165"
                     /old_locus_tag="GTK51_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821153.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]; GO:0008324 - monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_process="GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_000083351.1"
                     /translation="MSQSHAHDHGHDHDHDHTPTVTSANERKVLVSFFLIFGFMLVEAV
                     GGVLSGSLALLADAGHMLTDAVALALAYAAFRFGRRAADSKRTFGYLRFEVIAGFLNAV
                     TLFAIVAWIAYEAWERLQAPPVILAGPMMIVAVLGLLINVLVLWIMTRGETDHVNVKGA
                     ILHVMGDLLGSVGAIVAAIVIYFTGWTPIDPILSVLVAALILRSAWKLLAKSIHILLEG
                     APEDASPEKVEQGLMSTVPGLAAVSHVHVWQLTSGRTMATLHVRPNVDEEARAVVKRVE
                     VVLREQFSIEHATVGIDWNSEADENICSLQPTTDRQDHSGHGHSEHNHDHDHSAPGHKH
                     "
                     /name="cation diffusion facilitator family transporter"
     gene            complement(<11411..11827)
                     /locus_tag="GTK51_RS23170"
                     /old_locus_tag="GTK51_22885"
                     /pseudo=""
     CDS             complement(<11411..11827)
                     /locus_tag="GTK51_RS23170"
                     /old_locus_tag="GTK51_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /name="dihydropteroate synthase"
     ARG             7849..9002
                     /name="tet(X4)"
                     /gene="tet(X4)"
                     /gene_name="tetracycline-inactivating monooxygenase
                     Tet(X4)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(11313..11827)
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide resistant sul"
                     /tool="rgi"
                     /drug_class="sulfonamide antibiotic"
                     /identity="100.0"
                     /coverage="63.1"
     Typing          complement(3422..3578)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.671"
                     /coverage="100.0"
                     /evalue="1.08e-59"
     Typing          complement(74..895)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.813"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(1207..3332)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.769"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agggcacgga acaggccggg ggccagacag tgcgccgggt cgtgccggac gtggctgagg
       61 ctgtgcttgt tcttaggctt caccacgggg cacccccttg ctcttgcgct gcctctccag
      121 cacggcgggc ttgagcaccc cgccgtcatg ccgcctgaac caccgatcag cgaacggtgc
      181 gccatagttg gccttgctca caccgaagcg gacgaagaac cggcgctggt cgtcgtccac
      241 accccattcc tcggcctcgg cgctggtcat gctcgacagg taggactgcc agcggatgtt
      301 atcgaccagt accgagctgc cccggctggc ctgctgctgg tcgcctgcgc ccatcatggc
      361 cgcgcccttg ctggcatggt gcaggaacac gatagagcac ccggtatcgg cggcgatggc
      421 ctccatgcga ccgatgacct gggccatggg gccgctggcg ttttcttcct cgatgtggaa
      481 ccggcgcagc gtgtccagca ccatcaggcg gcggccctcg gcggcgcgct tgaggccgtc
      541 gaaccactcc ggggccatga tgttgggcag gctgccgatc agcggctgga tcagcaggcc
      601 agcggccacg gcttgccgct gctcgtcgtt gaggtatgcg cccagggcgt gaagccggtg
      661 atggatggcg gtgggtggat cttcggcggg caggtagagc accgggccgg tgggcagttc
      721 gcccacctcc agcagatccg gcccgcctgc aatctgtgcg gccagttgca gggccagcat
      781 ggatttaccg gcaccaccgg gcgacaccag cgccccgacc gtaccggcca ccatgttggg
      841 caaaacgtag tccagcggtg gcggcgctgc tgtgaacgcc tccagaatat tgataggctt
      901 atgggtagcc atgattgcct cctgttcagg cagttggtgg ttaggcgctg gcggggtcac
      961 tacccccgcc ctgcgccgct ctgagttctt ccaggtacag acgcagcgcc tcggcctcgt
     1021 cgtctgtggc ccatatctta cgggccttgc ggcccagttt tcgctgagcg tcggcaaacc
     1081 tagcctgcct gatcgcgttg ggcgtggcca acaggttgcc ggtctgcgtg tcccgatggt
     1141 ctttcatctc aaatcctcca ttagagctaa cagtatacta cccgttagcg ctaatgcaag
     1201 cgttacatgc tgaaatctgg cccgcccctg tccatgcctc gctggcgggg tgccggtgcc
     1261 tgtgccagct cggcccgcgc aagctggacg ctgggcagac ccataacctt gctgacggtg
     1321 cgctcgatgt aatccgcttc gtggccgggc ttgcgctctg ccagcgccgg gctggcctcg
     1381 gccatggcct tgccgatttc ctcggcactg cggccccggc tggccagttt ctgcgcggcg
     1441 ataaagtcgc acttgctgag gtcgtcaccg tagcgcttga ccagtccggc catctcgctg
     1501 cggtactcgt ccagcgccgt gcgccggtgg cggctaagct gccgctcggg cagttcgagg
     1561 ctggccagcc tgcgggcctt ctcctgctgc cgctgggcct gctcgatctg ctggccagcc
     1621 tgctgcacca gcgccgggcc agcggtggcg gtcttgccct tggactcacg cagcagcacc
     1681 cacggctgat agccggtgcg ggtggtgtgc ttgtccttgc ggttggtgaa acccgccaag
     1741 cggccatagt ggcggctgtc ggcgctggcc gggtcggcgt cgtactcgct ggccagcgtc
     1801 cgggcaatct gcccccggag ttcaccgcct gcggcgtcgg ccaccttgac ccatgcctga
     1861 tagttcttcg ggctggtctc cacgatcagc gccggttccc ggccctcggc tttcatgtcc
     1921 tccaggtcga gctcgctgag gtcgtccacc agcaccagac catggcgttc ctgctcggcg
     1981 ggcctgatat acacgtcatt gccctgggca ttcatccgct tgagccatgg cgtgttctgg
     2041 agcacttcgg aggctgacca ttcccggttc atcatctggc cggtggtggc atccctgacg
     2101 ccgatatcga agcgctcaca gcccatggcc ttgagctgtc ggcctatggc ctgcaaggtt
     2161 ctgtcgtttt tcatcgggcc accaagcgca gccagatcga gccgtcctct gttgtcagtg
     2221 gcgtcaggtc gagcaagagc aacgatgcga tcagcagcac cagcgtaggc atcatggaag
     2281 ccgccatcac ggttagccac agcttccagt gccaccctct ggccgcgctc cgggcgctct
     2341 gcgcggcgtc gctcacctcg ctggccactt cccgcaattc cttggccagc ttggcccatg
     2401 ctgcctgtgt ctcgcgctgg gctttcagcc actccgctgc ctgcgcctcg ctggcctgct
     2461 gggtctggct catgacctgc cgggcctcgt cggccagtgt cgccatgctc tgggccagcg
     2521 gctcgatctg ctccgctaac tcgttgattc ctctggattt cttcactctg tcgattgcgc
     2581 tcatggtcta tagcctcccg gtattcctgc aagtcgatga tctgggcgtt ggcggtgtcg
     2641 atgttcaggg ccacgtctgc ccggtcggtg cgaatgcccc ggccttccat ctccaccacg
     2701 ttcggcccca ggtgaacacc gggcaggcgc tcgatgccct gcgcctcaag cgttcggtgg
     2761 tcaatgcggg cgtcgtggcc agcccgctct aatgcccggt tggcatggtc ggcccatgcc
     2821 tcgcgggtct gctcaagcca tgccttgggc ttgagtgctt cggtcttctg cgctccgccc
     2881 ttctccgggg ccttgccgtt gtaccgcttg aaccactgag cggcgggccg ctcgatgccg
     2941 tcattgatcc gctcggagat catcaggtgg cagtgcgggt tctcgccgcc accggcatgg
     3001 atggccagcg tatacggcaa acgttcggca ccggtcaggt gctgggcgaa ctcggacgcc
     3061 agcgccttct gctggtcgag ggtcagctcg accggcaggg caaattcgac ctccttgaac
     3121 agccgcccat tggcgcgttc atgcaggtcg gcagcatccc agtagtcggc gggccgctcg
     3181 acgaactccg gcatgtgccc ggattcggcg tgcagcactt catccatgtc gcgggcatac
     3241 ttgccttcgc gctggatgta gtcggccttg gccttggccg attggccgcc cgacctgctg
     3301 ccggttttcg ccgtaaggtg ataaatcgcc atgctgcctc gctgttgctt ttgcttttcg
     3361 gctccatgca atggccctcg gagagcgcac cgcccgaagg gtggccgtta ggccagtttc
     3421 tcgaagagaa accggtaagt gcgccctccc gtacaaagta cggtcggggt ttgccgccgt
     3481 cgtgcctcca tgatagccta agagacagca cattaacaat gaggtgtcaa gatggctaag
     3541 gggagcaaca aggcggcgga taggctggcc aagctcgaag aacagcgagc acggatcaat
     3601 gccgagattc agcgggtgag ggcaagggag cagcagcaag agcgcaagaa cgaaacgcgg
     3661 cgcaaggtgc tggtgggcac catgattctg gccaaggtga acagcagcga gtggccagaa
     3721 gatcggctca tggcggcaat ggatgcgtac cttgaacgcg accacgaccg ggccttgttc
     3781 ggtctgccgc cacgccagaa ggatgagccg gcctgaattt aaactaataa aggaaagcaa
     3841 taatgactga tttaaaacta gacgattaca cagacgctga ttttattgat gaactgctca
     3901 gtgatgaact gcgctgtgaa tgcggagagg agcttcatta ttacgatgat ggttcattga
     3961 gtcgctgcaa ttcgtgtgat ccagatagag aaaccaatcc aaacctatga ttacctgcga
     4021 acattgcggg aaattagcag atgatgccga tgctgaatga tcgaccgaga caggccctgc
     4081 ggggctgcac acgcgccccc acccttcggg tagggggaaa ggccgctaaa gcggctaaaa
     4141 gcgctccagc gtatttctgc ggggtttggt gtggggttta gcgggctttg cccgcctttc
     4201 cccctgccgc gcagcggtgg ggcggtgtgt agcctagcgc agcgaataga ccagctatcc
     4261 ggcctctggc cgggcatatt gggcaagggc agcagcgccc cacaagggcg ctgataaccg
     4321 cgcctagtgg attattctta gataatcatg gatggatttt tccaacaccc cgccagcccc
     4381 cgcccctgct gggtttgcag gtttgggggc gtgacagtta ttgcaggggt tcgtgacagt
     4441 tattgcaggg gggcgtgaca gttattgcag gggttcgtga cagttattgc aggggggcgt
     4501 gacagttatt gcaggggttc gtgacagtta gtacgggagt gacgggcact ggctggcaat
     4561 gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac tttccgctaa gcgatagact
     4621 gtatgtaaac acagtattgc aaggacgcgg aacatgcctc atgtggcggc caggacggcc
     4681 agccgggatc gggatactgg tcgttaccag agccaccgac ccgagcaaac ccttctctat
     4741 cagatcgttg acgagtatta cccggcattc gctgcgctta tggcagagca gggaaaggaa
     4801 ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc aatgcgggcg gctggagcat
     4861 ggctttctac gggttcgctg cgagtcttgc cacgccgagc acctggtcgc tttcagctgt
     4921 aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga tggccgaaag tgccgccttg
     4981 ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt gggtgttgag cttcccgttt
     5041 cagctgcgtt tcctgtttgc cagccggccc gagatcatgg ggtgggtgct gggcatcgtt
     5101 taccgcgtca ttgccacgca cctggtcaag aaagcgggcc atacccacca agtggccaag
     5161 acgggcgcgg tcaccctgat ccagcgtttt ggatcggcgc tcaatctgaa tgttcacttc
     5221 cacatgctgt ttctcgacgg tgtgtatgtc gagcaatccc acggctcagc gcgtttccgc
     5281 tgggtcaagg cgccgaccag cccagagctc acccagctga cgcacaccat cgcccaccgg
     5341 gtgggtcgct atctggaacg gcaaggcctg ctggaacggg atgtcgaaaa cagctatctg
     5401 gcctcggatg cggtggatga cgacccgatg acacccctgc tggggcactc gatcacttac
     5461 cgtatcgctg tcggttcaca ggcggggcga aaggtgttca ctttgcaaac tctgccgacc
     5521 agtggtgatc cgttcggtga cgggattggc aaggtagccg ggtccagcct gcacgccggc
     5581 gtggcggcca gggccgatga acgcaagaag ctcgaacggc tgtgccggta catcagccgc
     5641 ccggcggtat ccgagaagcg gctgtcgtta acacgaggcg gcaacgtgcg ctaccagctc
     5701 aagacgccgt accgggacgg caccacgcac gtcattttcg aaccattgga tttcattgca
     5761 aggctggccg ccctggtacc gaagcccaga gtcaacctaa cccgcttcca cggggtgttc
     5821 gcacccaaca gtcggcaccg ggcgttggtc acgccggcaa aacggggcag gggcaacaag
     5881 gtcagggtgg ctgatgaacc ggcaacacca gcacaacggc gagcgtcgat gacatgggcg
     5941 caacggctca agcgtgtttt caatatcgac atcgagacct gcagcggctg cggcggcgcc
     6001 atgaaagtca tcgcctgcat tgaagaccct atagtgatca agcagatcct tgatcacctg
     6061 aagcacaaag ccgaaaccag cgggaccagg gcgttacccg aaagccgggc gccaccggct
     6121 gagctgctcc tgggtctgtt tgactgacga gcctgaaggc caacgatacc aatcaaaatg
     6181 ctgcgttcac agcgccgcgg cagggatccg tcgtgctggt tgtcggaaaa ggagccgcta
     6241 gtgggaaaga ggagggtaaa ttttcagcgt tgctggctcc ccgtcagccg gattgggttg
     6301 catcgcaggg gtgtcgaaag agtcaactgc ggtccaaagc tgttggactt gggtgaaaag
     6361 ggcgtttatt cttcctatac gttgtcggca gcgggccaaa aaggaatacg tccatgccca
     6421 tcgaggtgaa accggctgtg agcgcgggtt caagcatata gcccgacagg cgcgtatcct
     6481 tgccgatcac gacacgatgg cggtggtcac cgcgacgaaa gacacggcca gccgccatgc
     6541 cgacgcgcaa ggcggtttcc gccgtcatcg cgccttcgtt ggctttgcca cgaataccgt
     6601 ctgtgccgaa atatttgcgc accataaggt cgattatcct gtcgtcgggt cgccctcaaa
     6661 ggggacatgc ctgctgaacc gcgaatatag agaaatatcc cgaatgtgca gttaacgaat
     6721 tcttgcggtt tctttcagcg ccgccaatac cgccagcccg tcgcgcaagg ggcgcggctc
     6781 gtgtgtgcgg atgaagtcag ctccacctgc ggcggcggca aatagtaaaa acattcgcct
     6841 atcttgccat atgggtatga aatagagtag aatgtttcgt atatatataa gttagcgggc
     6901 attgtgaaag ataccagcat aacagttaaa cattgataaa tgaaaaaaaa aactactttg
     6961 gatattaatt ttaggactga taataatcag ttgcaaacaa aggaaaacag aaatgaaaga
     7021 gaaaataatt aaaacaaacg gcattgaact ctgtacggaa agttttggaa ataagaaaaa
     7081 tccagcaatc cttttggtag caggtgcaac cgtatcaatg ctgtattggg acactgaatt
     7141 ttgccaacaa ttatctgaaa aaggattttt tgttattcgt tacgacaaca gagatgtagg
     7201 aaaatccact aattatgagc cgggttcagc accatacgac attgttgact taacaaatga
     7261 tgccattgca attttggacg gctatgaaat agacaaagca cattttgtgg gtatgtcatt
     7321 aggtgggcta attacacaaa tagcagctat aaagtttgcc gatagagtta gctcattaac
     7381 acttatgtct acaggccctt ggggagactc ggacccaact attcctgaga tggacacacg
     7441 tattttagat ttccatggta aagcaggcac agtcgattgg tcaaatgaag acagtgtggt
     7501 aaactattta attcagggtg cagaattaat gtgcggcaag aaacaatttg ataaacaaag
     7561 aagtgaaaag ctgataagag ctgagttcaa tagagcaaac aattatataa gtatgttcaa
     7621 tcacgctaca ttgcaaggta gtgaagaata ttggaataga ctaaatgaag taaatcaacc
     7681 aaccttgata attcacggaa cagatgacaa aatttggcac tataaaaacg ctggtgtatt
     7741 aattgagaaa atacaaaata cgaaattaat tactcttgaa ggaacaggac acgaattgca
     7801 ctttgaagat tggacaacaa taattgacgg aattgaaaaa catataaatg agcaataaag
     7861 aaaaacaaat gaatttactt agtgataaga acgttgcaat aattggtggt ggacccgttg
     7921 gactgactat ggcaaaatta ttacagcaaa acggcataga cgtttcagtt tacgaaagag
     7981 acaacgaccg agaggcaaga atttttggtg gaacccttga cctacacaaa ggttcaggtc
     8041 aggaagcaat gaaaaaagcg ggattgttac aaacttatta tgacttagcc ttaccaatgg
     8101 gtgtaaatat tgctgatgaa aaaggcaata ttttatccac aaaaaatgta aagcccgaaa
     8161 atcgatttga caatcctgaa ataaacagaa atgacttaag ggctatcttg ttgaatagtt
     8221 tagaaaacga cacggttatt tgggatagaa aacttgttat gcttgaacct ggtaagaaga
     8281 agtggacact aacttttgag aataaaccga gtgaaacagc agatctggtt attattgcca
     8341 atggtggaat gtctaaagta agaaaatttg ttaccgacac ggaagttgaa gaaacaggta
     8401 ctttcaatat acaagccgat attcatcatc cagaggtgaa ctgtcctgga ttttttcagc
     8461 tatgcaatgg aaaccggcta atggctgctc atcaaggtaa tttattattt gcgaatccta
     8521 ataataatgg tgcattgcat tttggaataa gttttaaaac acctgatgaa tggaaaaacc
     8581 aaacgcaggt agattttcaa aacagaaata gtgtcgttga ttttcttctg aaagaatttt
     8641 ccgattggga cgaacgctac aaagaactga ttcgtgtgac atcatctttt gtagggttag
     8701 cgacacgaat atttccctta ggtaagtctt ggaaaagtaa gcgtccatta cccataacga
     8761 tgattggaga tgctgctcat ttgatgcctc cttttgcagg acaaggcgta aacagcgggt
     8821 tgatggatgc cttgatattg tcggataatc tgaccaatgg gaaatttaac agcattgaag
     8881 aggctattga aaattatgaa cagcaaatgt ttatctatgg caaagaagca caagaagaat
     8941 caactcaaaa cgaaattgaa atgtttaaac ccgactttac gtttcagcaa ttgttaaatg
     9001 tataaaatgg aataaaacgg cacataatcg agtagacggc tccgccagta actgacggag
     9061 tgcgcctctc acaccaccga gcgtacgggt cacgtactcg gcggttcgca aagagatggg
     9121 ttaagttgta aatgtaattc agttaaggaa acatatcctc ttttcttcag gcgttgtaaa
     9181 gtaatggtag tgcctagaat aggactttgt gccaactatt gagtgggagt gacgggcact
     9241 ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac tttccgctaa
     9301 gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc atgtggcggc
     9361 caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac ccgagcaaac
     9421 ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta tggcagagca
     9481 gggaaaggaa ttgccgggct atgtggggtt ctaggcattc agcaaggcgc gtcggtgttt
     9541 ctctagattg atagccaaaa aaaagcctac cccttgaagg agtaggccgg cgcgcactca
     9601 cacgttcagt caatcgtcgt ttctgtcctc cgaaatgtga accgccatgt cctgcagtac
     9661 ctggctgacg tgctggtcgg cgatgccgta gaagatgtgc ttggcctggc gctcgccgcg
     9721 cacgaggcgc gcgccgcgca acaggcgcag gtgatggctg accaaggatt gcgacaggtc
     9781 tagcgaatcg gcaatgtcgc ccactgcgac ggatccgcgc atgcactgca acaggatttt
     9841 caatcgcgat tggtcgccca gcaggcggaa ggtctcggcc agaacagcga cgtcactttg
     9901 cgacagtgcg aggacatcag tggtcgcgcc gtggctggag gtcgaacagt gagcaatagc
     9961 ggagctggtg gttttgcgtg atgtcatgga ttcaaaccct caagtgaact gcgagccggc
    10021 tcgcacggcc tcaatgcttg tgaccaggag cggagtggtc atggtcatga ttgtgctcac
    10081 tgtggccatg gccgctgtgg tcctgacggt ctgtcgtggg ttgcaggctg cagatgttct
    10141 cgtcagcctc cgagttccag tcgattccga ctgtggcgtg ttcgatgctg aattgctctc
    10201 gaagcaccac ctcgacgcgc ttgaccacgg ctcgcgcctc ctcgtccaca ttgggtcgaa
    10261 cgtgcagtgt ggccatcgtg cgaccggaag tgagttgcca cacatgaacg tggctgacag
    10321 ctgccaggcc gggtacagtg ctcatcaggc cttgttccac cttttccggc gaagcatcct
    10381 ctggcgcgcc ttccagcagg atatggatcg acttggccag gagcttccag gcgctgcgca
    10441 gaatcaacgc tgccaccaag accgacagaa tggggtcgat cggcgtccag ccggtgaaat
    10501 agatgacgat cgctgcaacg atggcgccga cagagcccag caggtcaccc atcacgtgga
    10561 gaatggcacc tttcacattg acgtgatcgg tttcgccgcg ggtcatgatc cacagcacga
    10621 ggacgttgat cagtaagccg agcacggcga cgatcatcat cgggccggcg aggatgacgg
    10681 gcggcgcctg cagccgctcc cacgcctcgt aggcgatcca tgcgacgatc gcgaacaggg
    10741 tcacggcgtt caggaagccg gcgatgacct cgaagcgcag gtaaccgaat gtgcgtttgc
    10801 tgtcggctgc gcggcgaccg aatcggaagg cagcgtaagc cagggcaagt gccacggcgt
    10861 cggtcagcat gtggccagca tcggcaagta gggctagcga gcccgacagc acgccgccga
    10921 cagcttccac gagcatgaag ccgaagatca ggaagaagga aaccagaacc ttgcgctcgt
    10981 tggcgctggt cactgtgggg gtgtggtcgt gatcatggtc gtgaccatgg tcatgtgcat
    11041 gggattggga catgggcgcc tctgctggtt gagtgtttga ctatggtgaa aatataacac
    11101 atgaatacat gttcatgtga agtattttcg ctcacccttc ccagccgccg aagccatacg
    11161 ttgtggtgcc gcaacgagaa aatgtgccta tcctttcggc cagatacggt gcaggtcatc
    11221 cacaatcagc tggtgatggt gcttgcctcg gtctgcgtgt gtgcgtaaat gagggtgatc
    11281 cggcggcaag ctggggtggt catgcaccac gtcagaaggg tcgctggccg gccacagcca
    11341 gagcgccagc accacgccgg ccaagccgat cagagacatg acaatgaagg tcgatggtaa
    11401 gcccatgacc gcgccgatct gcctgcccgt cttgcaccga atgcataacg acgagtttgg
    11461 cagatgattt cgccaattgc ggatagaacg cagcgtctgg aaaaccgcga atatcattga
    11521 gataggccac accacgcgac aaggcatagg cttgcgtcgc gggttgataa ctgtcgagcg
    11581 agacgggaat gccatctgcc ttgagcgcgt ccagcaccgg cgcgatacgc gcgatttctg
    11641 tgtcggacga aacaggcgcg gcgtcgggat tgctggatgc cggaccgagg tcgatcacat
    11701 ctgccccctc ggccatcagc ttacgcgcct gcgcaatggc tgcgtctggc gccagatacc
    11761 ggcctccatc ggagaaactg tccgaggtta tgttgacgat gccgaaaatg atgagcgatt
    11821 tattcatggg ggcttctata ataataataa tcgagcatga gtctcatacg gatgctcggg
    11881 tcgaaaggga atccccaggc gagtaacctg tttgcggtga tccattagct gcaggagcag
    11941 aatagcataa gccaggaaag cggcctatgg agctgtgcgg cagcgctcag taggcaattt
    12001 ttcaaaatat tgttaagcct tttctgagca tggtattttt catggtatta ccaattagca
    12061 ggaaaataag ccattgaata taaaagataa aaatgtcttg tttacaatag agtggggggg
    12121 gtcagcctgc cgccttgggc cgggtgatgt cgtacttgcc cgccgcgaac tcggttaccg
    12181 tccagcccag cgcgaccagc tccggcaacg cctcgcgcac ccgctggcgg cgcttgcgca
    12241 tggtcgaacc actggcctct gacggccaga catagccgca caaggtatct atggaagcct
    12301 tgccggtttt gccggggtcg atccagccac acagccgctg gtgcagcagg cgggcggttt
    12361 cgctgtccag cgcccgcacc tcgtccatgc tgatgcgcac atgctggccg ccacccatga
    12421 cggcctgcgc gatcaagggg ttcagggcca cgtacaggcg cccgtccgcc tcgtcgctgg
    12481 cgtactccga cagcagccga aacccctgcc gcttgcggcc attctgggcg atgatggata
    12541 ccttccaaag gcgctcgatg cagtcctgta tgtgcttgag cgccccacca ctatcgacct
    12601 ctgccccgat ttcctttgcc agcgcccgat agctaccttt gaccaccatg gcatcagcgg
    12661 tgacggcctc ccacttgggt tccaggaaca gccggagctg ccgtccgcct tcggtcttgg
    12721 gttccgggcc aagcactagg ccattaggcc cagccatggc caccagccct tgcaggatgc
    12781 gcagatcatc agcgcccagc ggctccgggc cgctgaactc gatccgcttg ccgtcgccgt
    12841 agtcatacgt cacgtccagc ttgctgcgct tgcgctcgcc ccgcttg
//
