LOCUS       NZ_CP047465             1749 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain ZF34 plasmid pZF34-1.7kb, complete sequence.
ACCESSION   NZ_CP047465
VERSION     NZ_CP047465.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13743100
            Assembly: GCF_011045895.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Peng,K. and Li,R.
  TITLE     The genomic epidemiology of tigecycline resistance gene tet(X)
            variants in a swine farm in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2020) College of Veterinary Medicine, Yangzhou
            University, No. 567, Wenchang Middle Road, Hanjiang District, Yangz,
            Yangzhou, Jiangsu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011045895.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:39:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,315
            CDSs (with protein)               :: 4,315
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 70 of 247
            Pseudo Genes (incomplete)         :: 181 of 247
            Pseudo Genes (internal stop)      :: 33 of 247
            Pseudo Genes (multiple problems)  :: 35 of 247
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047465.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ZF34"
                     /isolation_source="a feces sample of swine origin"
                     /db_xref="taxon:562"
                     /plasmid="pZF34-1.7kb"
                     /geo_loc_name="China: Nantong, jiangsu province"
                     /lat_lon="31.41 N 120.11 E"
                     /collection_date="2018-05"
                     /collected_by="College of Veterinary Medicine, Yangzhou
                     University"
     gene            join(1135..1749,1..375)
                     /locus_tag="GTK64_RS22530"
                     /old_locus_tag="GTK64_22450"
     CDS             join(1135..1749,1..375)
                     /locus_tag="GTK64_RS22530"
                     /old_locus_tag="GTK64_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039022372.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDSRWMFLTLTVRNCAIGELGDTLSRMNTAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKIEVKKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          complement(104..293)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.859"
                     /coverage="99.0"
                     /evalue="3.7200000000000003e-85"
ORIGIN
        1 gatccgaatg tcgatgtccg ggcagtgaaa ccccggaagc cgaaggacgg cgaatcgctg
       61 gcctgcgcga ccgccgaact ggtgcgcggc gcggtggccg agaccctgaa atattccacg
      121 aagccggccg acatggtcgc cgatcctgaa tggttcctcg aactgacccg ccagacgcac
      181 aagcgccgct ttgtggcgac cggcggcgca ctcaaagacg tattgaaact cgatcaggaa
      241 accgatgccg acatggtgat cggggatgat atttccgagg gcgatgatga cggttcccgc
      301 atcgcgttcg agtggaaaat cgaggtcaaa aaataccggc gttcgccgac gaaagataaa
      361 accgaatcag agtaaaaaca ctgcgatgca ggatagggca aacgccgcaa aatgacgtca
      421 tccgacatca ttccgccgcg ttcgcccagt gacctgcagt cggctctggg gtgtcggggc
      481 tgcgccctga cctggggttt tagattgcgg ccatcgtgat ggcggtaata caaaacgcac
      541 gggacaagat gtgtttttgg agtaccgccg acacgcgacg gacagtccaa aatatcttgg
      601 tcagggctgc gccccgacac cctgccaaca cctcacgccg ccagattaaa atctgccagc
      661 gttcgccgtt cctgaaatcg tcaaacgcta aatcgtgggg tcccgctggc ttgccagtgg
      721 gttccgattt agtgattgac gagcgcaccc gtaaaacact gccgacaggt ggttgtgatc
      781 acgcagtgag cgcaaccacc tgataagcgt ttttggggtt tgtggggtcc gatttaacgt
      841 ggattttggg ggcaaaaaaa tcgagcgcgt tccttgtatt tatacttaag ggataactct
      901 ggcgttaaga ataagcctca gcccttgtgc cgcaagggtt tcagcgtgat ttgcctgaaa
      961 atgactttga agttattctg tcgggagtgg tcgcgttaaa agcgaccccc gcaacgacgg
     1021 ccatcgttcg ggggcgtgac cacctgtagg aattctattt tgcgacagac aacacaggtg
     1081 atacggcaat gataacacgt caagtgagcc atcgcccagt atggggcggt gcgcatgaaa
     1141 aatcctgaac tccagaacct cacggactat tccccctcag atgcgccctg ggacgctcac
     1201 agaagcgttt ctgacgacgt gggagggatt tacctgcttg cggccgaata tgagcgctac
     1261 ggggcgcgta tggcctcctg cggcggttta ctgcgtttcg ggtggtctac cctcaaagaa
     1321 accggagaaa cgcgcctgag gctgcgtgag gcgcatttct gccgtgtccg gcactgtcct
     1381 gtctgccagt ggaggcgctc cctgatgtgg caagcccgtt tctaccagtc cctgccgcgt
     1441 atcgtggcgg attaccccga ttcccgctgg atgttcctga ccctgaccgt gcgcaactgt
     1501 gcgatcgggg agctgggcga tacgctgagc cggatgaaca cggctttcca gcggctgaag
     1561 gatcgcaaag agttcagacc agttcaggga tggatccgca ccacggaagt cacccgcggc
     1621 agtgacggca gcgcacaccc gcacttccac acactgatga tggttccgcc tggcatgctt
     1681 aacgggaaga gttacgtcaa acatgcgcgc tgggttgagc tgtggcggga gtgccttcgg
     1741 gtcagctac
//
