LOCUS       NZ_CP047462             7542 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain ZF34 plasmid pZF34-7kb, complete sequence.
ACCESSION   NZ_CP047462
VERSION     NZ_CP047462.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13743100
            Assembly: GCF_011045895.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Peng,K. and Li,R.
  TITLE     The genomic epidemiology of tigecycline resistance gene tet(X)
            variants in a swine farm in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2020) College of Veterinary Medicine, Yangzhou
            University, No. 567, Wenchang Middle Road, Hanjiang District, Yangz,
            Yangzhou, Jiangsu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011045895.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:39:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,684
            CDSs (total)                      :: 4,562
            Genes (coding)                    :: 4,315
            CDSs (with protein)               :: 4,315
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 70 of 247
            Pseudo Genes (incomplete)         :: 181 of 247
            Pseudo Genes (internal stop)      :: 33 of 247
            Pseudo Genes (multiple problems)  :: 35 of 247
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047462.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ZF34"
                     /isolation_source="a feces sample of swine origin"
                     /db_xref="taxon:562"
                     /plasmid="pZF34-7kb"
                     /geo_loc_name="China: Nantong, jiangsu province"
                     /lat_lon="31.41 N 120.11 E"
                     /collection_date="2018-05"
                     /collected_by="College of Veterinary Medicine, Yangzhou
                     University"
     gene            join(6390..7542,1..113)
                     /locus_tag="GTK64_RS22445"
                     /old_locus_tag="GTK64_22380"
     CDS             join(6390..7542,1..113)
                     /locus_tag="GTK64_RS22445"
                     /old_locus_tag="GTK64_22380"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="WP_157914643.1"
                     /translation="MPNEIINIILDTNPDEINSNFFFSTKEIIGLLIIACNFLFILIGK
                     RDSKFQLRHFYIFIILFVSLLIAFKPLGLALKEINKGLSELKKNAQIIKDKKNFKWDSK
                     STINDKQTVVLFLGETNRGDYLHFNGYPKNTTPNLDKENLVSFKNTISQGAYTLVSTPM
                     ILSRKGVYDNGLFNETSIISAYKEAGYETWYVSYLSKSHIGDNEINLIANEADHYVVSD
                     VNVNTLEKILNNKSKKKLIVYKTIGSHYLYHTRYPSNYDVFKPSFNEKTYSTPSLKDKD
                     KLENSYANSELFSVDKQISDFINVLKNTDGLAYLSFISDHGTAIYDDGKSLYGGNTKGN
                     YNIAHFFWFNDLYHKQHPELIQKLSINKDKKITSECFVDTSLELSFIESKIKKGCSLLN
                     DKFIEKQRLVKNGKVYDFDQDL"
                     /name="phosphoethanolamine transferase"
     gene            complement(385..>450)
                     /locus_tag="GTK64_RS23550"
                     /pseudo=""
     CDS             complement(385..>450)
                     /locus_tag="GTK64_RS23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            836..1399
                     /locus_tag="GTK64_RS22450"
                     /old_locus_tag="GTK64_22385"
     CDS             836..1399
                     /locus_tag="GTK64_RS22450"
                     /old_locus_tag="GTK64_22385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602744.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="WP_072666782.1"
                     /translation="MPKNVFFTLRNRKSHELKKTLLSIALLTASITALSGCQSNADQYA
                     ADVYDTGQLNAKQETKTVNIISVLPAKVAVDNTANKQAAQTFGAILGAVVGGVAGHNVG
                     SGSGLCTTAGAVGGGAVGAAVGTLVKDKSIVEGVSLTYKEGTKVFTSTQVGKPCQFTTG
                     LAVVISTKNNETRIQPNTTCPVKK"
                     /name="glycine zipper 2TM domain-containing protein"
     gene            1410..1946
                     /locus_tag="GTK64_RS22455"
                     /old_locus_tag="GTK64_22390"
                     /pseudo=""
     CDS             1410..1946
                     /locus_tag="GTK64_RS22455"
                     /old_locus_tag="GTK64_22390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /name="DUF5384 family protein"
     gene            complement(2480..2695)
                     /locus_tag="GTK64_RS22460"
                     /old_locus_tag="GTK64_22395"
     CDS             complement(2480..2695)
                     /locus_tag="GTK64_RS22460"
                     /old_locus_tag="GTK64_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2702..3193)
                     /locus_tag="GTK64_RS22465"
                     /old_locus_tag="GTK64_22400"
     CDS             complement(2702..3193)
                     /locus_tag="GTK64_RS22465"
                     /old_locus_tag="GTK64_22400"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_140428842.1"
                     /translation="MSNLLTLAKDLEQQSKAQQKSTGEMLKAAFCEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMKTEAIPYGGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<3234..3932)
                     /locus_tag="GTK64_RS23755"
                     /old_locus_tag="GTK64_22405"
                     /pseudo=""
     CDS             complement(<3234..3932)
                     /locus_tag="GTK64_RS23755"
                     /old_locus_tag="GTK64_22405"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3871..4194)
                     /locus_tag="GTK64_RS22475"
                     /old_locus_tag="GTK64_22410"
     CDS             complement(3871..4194)
                     /locus_tag="GTK64_RS22475"
                     /old_locus_tag="GTK64_22410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_170915627.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNTGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4832..4963
                     /locus_tag="GTK64_RS23560"
     CDS             4832..4963
                     /locus_tag="GTK64_RS23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_161992909.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPKRHDLTPLAAATGRGCTRDY"
                     /name="replication initiation protein"
     gene            complement(5265..5962)
                     /locus_tag="GTK64_RS22480"
                     /old_locus_tag="GTK64_22415"
     CDS             complement(join(5265..5713,5713..5962))
                     /locus_tag="GTK64_RS22480"
                     /old_locus_tag="GTK64_22415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_225905514.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCCKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     Typing          complement(4296..4482)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="85.714"
                     /coverage="86.0"
                     /evalue="2.1000000000000002e-48"
     Typing          4428..4940
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.039"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2389..3881)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.4"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3878..4194)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="6.609999999999999e-60"
ORIGIN
        1 cctttattga aagtaagatt aaaaaaggtt gttcactact aaacgataaa tttattgaaa
       61 aacagcgatt ggtgaaaaat ggtaaagttt atgatttcga ccaagattta taaaatgatt
      121 tccatataat tgaactagct aaaacttaac ctggcagtta cttattttta ttaggtgtct
      181 gtcaggttaa atatgtttac taagttatac aaaatataag acactgttgc aaagttaggg
      241 aaggtgcgaa taagcgggga aattcttctc ggctaactca gtcatttcat ttcttcatat
      301 ttgagccgat tttttctccc gtaaatgcct tgaatcagcc tatttagacc gtttcttcgc
      361 catttaaggc gttatcccca gtttttagtg agatctctcc cactgacgta tcatttggtc
      421 cgcccgaaac aggttggcca gcgtgaataa ggggtgagtt tggagattgg aaattattgt
      481 acgttaagcc tgttttttga acagatcgcg ctatcgcaat agggtgtact cctgcactat
      541 acggcattta gcctgtatgt ttgggtaatg agacaaccta aatgcgacag cgtgagcgca
      601 ataacttaaa tgcgatagtc agtaacaata aatcaatcag ttaccaatca gaaacggtta
      661 aattatgttc ttaacgtaca ataatttcca tttcccaaac cgaccccagg aaggacatct
      721 gatggaaatg gaagccactg gagcacctca aaaacaccat catgcactaa atcagtaagt
      781 tggcaccatt accggaaaat caaccaccac ctttagcctt tactttttac ggataattcc
      841 taaaaatgtt ttttttacat taagaaacag gaagtcacat gagctcaaaa aaacactgtt
      901 aagcatagcc ctactaaccg cctcgattac cgctttatca ggctgtcaat ccaatgccga
      961 tcaatatgct gctgacgttt atgacacagg gcaactgaat gcaaaacagg agacgaaaac
     1021 tgtaaacata atttctgttt tgcctgcgaa ggttgctgtt gataacactg caaacaaaca
     1081 agctgcacaa acctttgggg caatccttgg cgctgtggtt ggcggtgtgg ctggtcataa
     1141 cgtcggaagt ggtagcggac tttgcacaac agccggagcc gttggcggtg gtgccgtcgg
     1201 tgccgcagta ggaacattag tcaaagataa atctatcgtt gaaggtgttt ctttaactta
     1261 caaagaaggg acaaaggtat ttacatcaac tcaagtagga aaaccatgtc agtttactac
     1321 tggtttagct gttgttattt ccacaaaaaa taatgaaaca agaatccagc caaacacaac
     1381 atgcccggta aaaaaataag attcaaacga tgaaaagaat cattataccc ctcatccttt
     1441 cttttagtgg aagcagcttt gcaaactcac ttcaagaaca attatccgca gtagctcaag
     1501 ctgaacagca ggggaaagct gaagagcaaa gactcataga tatccagaga gaaaaaattg
     1561 aggaaaacaa acggcttgag caaaaaagga gagaaagagc aagagcagaa gcgaacagga
     1621 gagcagccat agcttctgca gagagaaaag ctcaccgtga aagaatcgaa gctgagctca
     1681 aagccgataa aaatagagat cagaaatatg aagatgaact aagaaagctt gaaatcgaaa
     1741 aacaaaagtt agcattagcc agggaagagg caagagttaa acacgaaaat taattcatag
     1801 accaagagtt aaaaaacaag gcagcccaaa cagatgtaat tcaatctcac gccgactcaa
     1861 acagaaatat ttcagaaggc gggaaagatt taatgaaaag cgaagggaaa gccagagaaa
     1921 aagaagctgg aggatggttt aactagcttc atttttataa ttaaagttga tttcaagttt
     1981 aattaacaac ggatgttatt taccataaca tctgttgccc gcaccgctcc ggacgggttc
     2041 gccacaaacg gggcggcagt caggtaaaac cggcaacaaa ccacccgata caccgccccg
     2101 ttcgtgacag ataaatttta cccgactgca tagccatgca gccagatgca tggcgtgtaa
     2161 agtcacacta aaatcagcgc gaacgcctta aaaacaggta aatgcttctc cagccagtcc
     2221 cacagcccgg ccgccagata caggcagtta agtacagctt caatgacgtt aagcaatttc
     2281 ataattcccc cccaggtata ggccgtgatc tttttaatca cgaacctatt tagcgggaga
     2341 agtaagagcc cctcttcgtg cgggttgtca gctgcctgga tggtttaaag tgtcggcccg
     2401 cggtaggttt tctgcggctg gcgctccagc tccagtgttt tctgatactc attctccctc
     2461 tgccggagct cgatggcatt cagttcccgg acaagcggcg cagctgctcg cttaaactca
     2521 gcacctgcgt gctcaagtcc gacacgcgct cgttcagcac cgcgttctcc cgttgcataa
     2581 gcccacacat gctccgccat tccgcgaagg cgctctccca ttcgtccagc ctttgcgagt
     2641 agtcctgctg tagctgctct aatgcgctca gcaactgctt ttccagctct gtcatcttcc
     2701 gctactcctg tttcaggcgg atccatgtcg tcccgccgta agggatggct tcggttttca
     2761 tccccggcgg taggataaga aacctccggc cgtcgctgct ctcctgatag cgcactcccc
     2821 acgtccgggc ggtcattttc gccagggtat cttcctgctg acgaagatgc gtgtaattgt
     2881 cggttatctg ctgcccctgc caccacagta tgctcccgct cgtgccgatc agcagcgctg
     2941 acaccatcag gatggtgagc catgtccgcc cggcagtccg cagtacgctc cgccggtttt
     3001 tctccatcgc ctcgctcata ctctgctcgt gggcgaggat ggcgtcgctg attctcctcg
     3061 cgcttgcgct cagttccgct ctgacagact gctcgtgctc gcagaatgcg gctttcagca
     3121 tctcgccagt actcttctgc tgcgctttcg attgctgctc taagtccttc gctagcgtta
     3181 aaagattgct catagatagc tcccttcagt cggatgtttc gccccccgtc cgggtcggca
     3241 atgctgatac tgcttttcgt ggtgcgcacc accttaaaac ctgcaccttc aagagtgtca
     3301 gtgacatcct gacggctttt cagctccccg gacgaggcta agctcagtag tccccgggta
     3361 atggcttctg cggcctcctg cgtcgttttc ggcagcccgg acggcgtgac cagcgcccgg
     3421 cggttttccg gcgcgttcgg gtcgtgcagc cccagtctgc cgttcacgat agtctgccag
     3481 gcatcgatgc gcggccggtc ggcgcggtcg tagtaaggct ggagccgcct gccggtcagc
     3541 agctcggtat tggggatcag aaagttcagc tccagacgcc ccttgtccgc gtgctccacc
     3601 cacagaatgc tgtactgatc tttatcgagt ccgggcatca gaacccgctc gaagctggcc
     3661 atcagttttt cccgctggcc gggagggaga tcggcctccg caaacgacag gacgccggag
     3721 gtgtatttct taacgtaagg cgacgcgtca atcagctccc ggacttcttc cggcttcccc
     3781 tgcaggacgg tggcgccttc acgctgccgg tctttaccca gcagatagtc caccggcccg
     3841 gcgccaccgc cgcgaccgcg tggatgaaat ttaacgatca tcgtccgttc ctttctcaag
     3901 aacggcgtgc cgcagccgct caagtccggc atcgatggcc atcagcgccg ccacaatctg
     3961 taccctgtca tggccagtac cgccgccggt gttaacacgg cgggctatct ggttcaggtt
     4021 attgcccatg ccggcaagct ggcgcagcag cgccggcgaa attgagggga gctttccggt
     4081 gcgcgcaggc ttttcgtcca ggcaggtctg ccgcatccag gccgccagcg gcctgccgtc
     4141 acagcgctca agcagccgcc ggtgctcgtc ctccgtcacc cacatggtga gcatcttgtt
     4201 ccgtttctct gacaaaccgg cctcctgata acgccgggac gggcgggaaa gcctttcccg
     4261 gtcggcgcgg ggttggacaa gccgcaggcg cgtcaggctt ttggcgggtt tcggggcgca
     4321 gccctgaacc agtcacgtag cgctagcgga gtgtatactg gctcaatatg ttacggcgga
     4381 gtaacactta ataaaagtgc ttcatgtggc aggggaaaaa tgcagctgca gggcgtacag
     4441 gagaaaatgg aggttacggt gatatgacgc ctcctcgctc actcggtcgc ttccgctcct
     4501 gacgttcggc tgcggcgggc gttacctgca aacggataag agcaaaggca tacggcatgg
     4561 caagcaggac aggaagagga tacggagctg ccgtttttcc ataggctccg cccccctgac
     4621 gagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     4681 taccaggcgt ttccccctga tggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     4741 accggtgtca ttccgctgtt atggccgcgt ttatctcatt ccacgcctga cactcagctc
     4801 cgggtaggca gttcgctcca agctggactg tgtgcacgaa ccccccgttc agtccgaccg
     4861 ctgcgcctta tccggtaact atcgtcttga gtccaacccg aaaagacacg acttaacgcc
     4921 actggcagca gccactggta gagggtgtac aagagattac tgatgcagag ttcttgaagt
     4981 tagagccaaa ctacggctaa actggaagga cgaatttggt ggcagcggtc ttgtacaccc
     5041 tgttaccacg gttcagaagt tccccaactt actgaacctt cgaaaaacca cctccccagg
     5101 tggttttttc gtttctggat aagagactac gacgacagac ggacgatctc aagaaaatcc
     5161 tctctaacag gttatattca gagtaaaaaa agtgagctta aatatggctt tttcaatagc
     5221 agaagtgcgc gaggggcgta tcggttcttg gtaatgactc caacttactg atagtgtttt
     5281 atgttcagat aatgcccgat gaccttgtca tgcagctcca ccgattttga gaacgacagt
     5341 gacttccgtc ccagccttgc cagatgttgt ctcagattca gattatgtcg ctcaatgcgc
     5401 tgagtgtaac gcttgctgat aacgtgcagc tttcccttca ggcgtgattc atacagcggc
     5461 caaccatccg tcatccatac cacgacctca aaggccgaca gcaggctcag aagacgctcc
     5521 agtgtggcca gagtgcgttc accgaagacg tgcgccacaa ccgtcctccg tatcctgtca
     5581 tacgcgtaaa acagccagcg ctgacgtgat ttagcaccga cgtagcccca atgttcgtcc
     5641 atttcagcgc agacaatcac atcactgccc ggttgtatac gcgaggttac cgactgcggc
     5701 ctgagttttt taagtgacgt aaaaccgtgt tgaggccaac gcccataatg cgtgcactgg
     5761 cgcgacatcc gacgccattc atggccatat caatgatttt ctggtgcgta ccgggctgag
     5821 aggcggtgta agtgaactgt agttgccatg ttttacagca atgagagcag agatagcgct
     5881 gatgtccggc agtgcttttg ccgttacgca ccacgccttc agtagcggag caggaaggac
     5941 atctgatgga aatggaagcc atgcaagcac ctaaaaatca ccatcataca ctaaatcagt
     6001 aagttggcag cattaccgag agcggctttt taattaaata atgatttcct atgatttttt
     6061 ttttaaactt acttacttac ttacttactt acttatatct atgttatcaa ataatgaaca
     6121 tcatcaataa aatttcaaaa aaccatcaaa aaaacatata catagcttta tgtgttatta
     6181 atctaatttt tataattaat acttcccata gaataatcgc tagtaatatt tattactcct
     6241 taacgcttat catacttggt gttatttttt tccatggtta cacttcaaaa attaattcaa
     6301 ttatttcttt atcaatatca agttttatta tttcacttct atttgttgat ttaatgacat
     6361 atcgaatgac ctcctggtat tttcatcagg tgccaaatga aatcattaat attattcttg
     6421 atactaaccc agatgagatt aatagtaatt ttttcttttc aacaaaagaa attatagggc
     6481 tactaatcat agcatgtaac ttcttattta ttcttattgg aaaaagagac tctaaatttc
     6541 agttaaggca tttttatatt ttcattattc tgtttgtttc tttgttaatc gcctttaagc
     6601 cattaggatt agctttgaaa gaaattaata aaggtttatc tgaacttaag aaaaatgcac
     6661 aaattataaa agataaaaaa aacttcaagt gggattctaa atctacaata aatgataagc
     6721 aaacagtggt tcttttttta ggcgaaacta atagaggaga ttatttacat tttaatggtt
     6781 accctaaaaa cacaacccca aatttagata aagaaaattt agtttctttt aaaaacacaa
     6841 tatctcaagg agcatatact ttagtttcca caccaatgat cctttccaga aaaggtgtgt
     6901 atgataatgg tctttttaat gaaacatcaa taattagtgc ttataaagag gctggatatg
     6961 aaacttggta tgtttcttat ctgagtaaaa gtcacatagg agataatgaa ataaatttaa
     7021 ttgcaaatga agctgatcat tatgttgtat ctgatgtcaa cgtgaataca ttagagaaaa
     7081 tattaaataa taaaagcaag aaaaagctca tagtttataa aactatcggg agtcattatc
     7141 tgtatcatac aaggtatcca tcaaattatg atgttttcaa accatctttt aatgaaaaaa
     7201 catattcaac tccatcttta aaagataagg ataaattaga aaatagctat gctaattcag
     7261 aattattttc tgttgataaa cagataagcg atttcattaa tgtgttaaaa aatacagatg
     7321 gacttgcata tttatctttt atttcagacc atggaactgc tatttatgat gatggtaaat
     7381 cgttgtatgg cggtaataca aaaggcaact ataacattgc tcattttttt tggtttaatg
     7441 atttatacca taaacaacat cctgagctaa tacaaaaact ttcaattaat aaagataaaa
     7501 aaataacatc cgaatgtttc gtagatacta gtttagagtt at
//
