LOCUS       NZ_CP047459             1749 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain ZF31 plasmid pZF31-1.7kb, complete sequence.
ACCESSION   NZ_CP047459
VERSION     NZ_CP047459.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13742197
            Assembly: GCF_011045875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Peng,K. and Li,R.
  TITLE     The genomic epidemiology of tigecycline resistance gene tet(X)
            variants in a swine farm in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2020) College of Veterinary Medicine, Yangzhou
            University, No. 567, Wenchang Middle Road, Hanjiang District, Yangz,
            Yangzhou, Jiangsu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011045875.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:50:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,565
            Genes (coding)                    :: 4,318
            CDSs (with protein)               :: 4,318
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 68 of 247
            Pseudo Genes (incomplete)         :: 182 of 247
            Pseudo Genes (internal stop)      :: 33 of 247
            Pseudo Genes (multiple problems)  :: 34 of 247
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047459.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ZF31"
                     /isolation_source="a feces sample of swine origin"
                     /db_xref="taxon:562"
                     /plasmid="pZF31-1.7kb"
                     /geo_loc_name="China: Nantong, jiangsu province"
                     /lat_lon="31.41 N 120.11 E"
                     /collection_date="2018-05"
                     /collected_by="College of Veterinary Medicine, Yangzhou
                     University"
     gene            join(1351..1749,1..591)
                     /locus_tag="GTK48_RS22515"
                     /old_locus_tag="GTK48_22440"
     CDS             join(1351..1749,1..591)
                     /locus_tag="GTK48_RS22515"
                     /old_locus_tag="GTK48_22440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039022372.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDSRWMFLTLTVRNCAIGELGDTLSRMNTAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKIEVKKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          complement(320..509)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.859"
                     /coverage="99.0"
                     /evalue="3.7200000000000003e-85"
ORIGIN
        1 atgaacacgg ctttccagcg gctgaaggat cgcaaagagt tcagaccagt tcagggatgg
       61 atccgcacca cggaagtcac ccgcggcagt gacggcagcg cacacccgca cttccacaca
      121 ctgatgatgg ttccgcctgg catgcttaac gggaagagtt acgtcaaaca tgcgcgctgg
      181 gttgagctgt ggcgggagtg ccttcgggtc agctacgatc cgaatgtcga tgtccgggca
      241 gtgaaacccc ggaagccgaa ggacggcgaa tcgctggcct gcgcgaccgc cgaactggtg
      301 cgcggcgcgg tggccgagac cctgaaatat tccacgaagc cggccgacat ggtcgccgat
      361 cctgaatggt tcctcgaact gacccgccag acgcacaagc gccgctttgt ggcgaccggc
      421 ggcgcactca aagacgtatt gaaactcgat caggaaaccg atgccgacat ggtgatcggg
      481 gatgatattt ccgagggcga tgatgacggt tcccgcatcg cgttcgagtg gaaaatcgag
      541 gtcaaaaaat accggcgttc gccgacgaaa gataaaaccg aatcagagta aaaacactgc
      601 gatgcaggat agggcaaacg ccgcaaaatg acgtcatccg acatcattcc gccgcgttcg
      661 cccagtgacc tgcagtcggc tctggggtgt cggggctgcg ccctgacctg gggttttaga
      721 ttgcggccat cgtgatggcg gtaatacaaa acgcacggga caagatgtgt ttttggagta
      781 ccgccgacac gcgacggaca gtccaaaata tcttggtcag ggctgcgccc cgacaccctg
      841 ccaacacctc acgccgccag attaaaatct gccagcgttc gccgttcctg aaatcgtcaa
      901 acgctaaatc gtggggtccc gctggcttgc cagtgggttc cgatttagtg attgacgagc
      961 gcacccgtaa aacactgccg acaggtggtt gtgatcacgc agtgagcgca accacctgat
     1021 aagcgttttt ggggtttgtg gggtccgatt taacgtggat tttgggggca aaaaaatcga
     1081 gcgcgttcct tgtatttata cttaagggat aactctggcg ttaagaataa gcctcagccc
     1141 ttgtgccgca agggtttcag cgtgatttgc ctgaaaatga ctttgaagtt attctgtcgg
     1201 gagtggtcgc gttaaaagcg acccccgcaa cgacggccat cgttcggggg cgtgaccacc
     1261 tgtaggaatt ctattttgcg acagacaaca caggtgatac ggcaatgata acacgtcaag
     1321 tgagccatcg cccagtatgg ggcggtgcgc atgaaaaatc ctgaactcca gaacctcacg
     1381 gactattccc cctcagatgc gccctgggac gctcacagaa gcgtttctga cgacgtggga
     1441 gggatttacc tgcttgcggc cgaatatgag cgctacgggg cgcgtatggc ctcctgcggc
     1501 ggtttactgc gtttcgggtg gtctaccctc aaagaaaccg gagaaacgcg cctgaggctg
     1561 cgtgaggcgc atttctgccg tgtccggcac tgtcctgtct gccagtggag gcgctccctg
     1621 atgtggcaag cccgtttcta ccagtccctg ccgcgtatcg tggcggatta ccccgattcc
     1681 cgctggatgt tcctgaccct gaccgtgcgc aactgtgcga tcggggagct gggcgatacg
     1741 ctgagccgg
//
