LOCUS       NZ_CP047456             7542 bp    DNA     circular CON 25-JUN-2024
DEFINITION  Escherichia coli strain ZF31 plasmid pZF31-7kb, complete sequence.
ACCESSION   NZ_CP047456
VERSION     NZ_CP047456.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13742197
            Assembly: GCF_011045875.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Peng,K. and Li,R.
  TITLE     The genomic epidemiology of tigecycline resistance gene tet(X)
            variants in a swine farm in China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Peng,K. and Li,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JAN-2020) College of Veterinary Medicine, Yangzhou
            University, No. 567, Wenchang Middle Road, Hanjiang District, Yangz,
            Yangzhou, Jiangsu 225000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50.0x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_011045875.1-RS_2024_06_25
            Annotation Date                   :: 06/25/2024 03:50:15
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,565
            Genes (coding)                    :: 4,318
            CDSs (with protein)               :: 4,318
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 16
            Pseudo Genes (total)              :: 247
            CDSs (without protein)            :: 247
            Pseudo Genes (ambiguous residues) :: 0 of 247
            Pseudo Genes (frameshifted)       :: 68 of 247
            Pseudo Genes (incomplete)         :: 182 of 247
            Pseudo Genes (internal stop)      :: 33 of 247
            Pseudo Genes (multiple problems)  :: 34 of 247
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047456.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ZF31"
                     /isolation_source="a feces sample of swine origin"
                     /db_xref="taxon:562"
                     /plasmid="pZF31-7kb"
                     /geo_loc_name="China: Nantong, jiangsu province"
                     /lat_lon="31.41 N 120.11 E"
                     /collection_date="2018-05"
                     /collected_by="College of Veterinary Medicine, Yangzhou
                     University"
     gene            complement(join(6435..7542,1..158))
                     /locus_tag="GTK48_RS22430"
                     /old_locus_tag="GTK48_22370"
     CDS             complement(join(6435..7542,1..158))
                     /locus_tag="GTK48_RS22430"
                     /old_locus_tag="GTK48_22370"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="WP_157914643.1"
                     /translation="MPNEIINIILDTNPDEINSNFFFSTKEIIGLLIIACNFLFILIGK
                     RDSKFQLRHFYIFIILFVSLLIAFKPLGLALKEINKGLSELKKNAQIIKDKKNFKWDSK
                     STINDKQTVVLFLGETNRGDYLHFNGYPKNTTPNLDKENLVSFKNTISQGAYTLVSTPM
                     ILSRKGVYDNGLFNETSIISAYKEAGYETWYVSYLSKSHIGDNEINLIANEADHYVVSD
                     VNVNTLEKILNNKSKKKLIVYKTIGSHYLYHTRYPSNYDVFKPSFNEKTYSTPSLKDKD
                     KLENSYANSELFSVDKQISDFINVLKNTDGLAYLSFISDHGTAIYDDGKSLYGGNTKGN
                     YNIAHFFWFNDLYHKQHPELIQKLSINKDKKITSECFVDTSLELSFIESKIKKGCSLLN
                     DKFIEKQRLVKNGKVYDFDQDL"
                     /name="phosphoethanolamine transferase"
     gene            586..1283
                     /locus_tag="GTK48_RS22435"
                     /old_locus_tag="GTK48_22375"
     CDS             join(586..835,835..1283)
                     /locus_tag="GTK48_RS22435"
                     /old_locus_tag="GTK48_22375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_225905514.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCCKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEHWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            complement(1585..1716)
                     /locus_tag="GTK48_RS23550"
     CDS             complement(1585..1716)
                     /locus_tag="GTK48_RS23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_161992909.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPKRHDLTPLAAATGRGCTRDY"
                     /name="replication initiation protein"
     gene            2354..2677
                     /locus_tag="GTK48_RS22440"
                     /old_locus_tag="GTK48_22380"
     CDS             2354..2677
                     /locus_tag="GTK48_RS22440"
                     /old_locus_tag="GTK48_22380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_170915627.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNTGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2886..>3314
                     /locus_tag="GTK48_RS23555"
                     /pseudo=""
     CDS             2886..>3314
                     /locus_tag="GTK48_RS23555"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3355..3846
                     /locus_tag="GTK48_RS22450"
                     /old_locus_tag="GTK48_22390"
     CDS             3355..3846
                     /locus_tag="GTK48_RS22450"
                     /old_locus_tag="GTK48_22390"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_140428842.1"
                     /translation="MSNLLTLAKDLEQQSKAQQKSTGEMLKAAFCEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMKTEAIPYGGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            3853..4068
                     /locus_tag="GTK48_RS22455"
                     /old_locus_tag="GTK48_22395"
     CDS             3853..4068
                     /locus_tag="GTK48_RS22455"
                     /old_locus_tag="GTK48_22395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4602..5138)
                     /locus_tag="GTK48_RS22460"
                     /old_locus_tag="GTK48_22400"
                     /pseudo=""
     CDS             complement(4602..5138)
                     /locus_tag="GTK48_RS22460"
                     /old_locus_tag="GTK48_22400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /name="DUF5384 family protein"
     gene            complement(5149..5712)
                     /locus_tag="GTK48_RS22465"
                     /old_locus_tag="GTK48_22405"
     CDS             complement(5149..5712)
                     /locus_tag="GTK48_RS22465"
                     /old_locus_tag="GTK48_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602744.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="WP_072666782.1"
                     /translation="MPKNVFFTLRNRKSHELKKTLLSIALLTASITALSGCQSNADQYA
                     ADVYDTGQLNAKQETKTVNIISVLPAKVAVDNTANKQAAQTFGAILGAVVGGVAGHNVG
                     SGSGLCTTAGAVGGGAVGAAVGTLVKDKSIVEGVSLTYKEGTKVFTSTQVGKPCQFTTG
                     LAVVISTKNNETRIQPNTTCPVKK"
                     /name="glycine zipper 2TM domain-containing protein"
     gene            <6098..6163
                     /locus_tag="GTK48_RS23560"
                     /pseudo=""
     CDS             <6098..6163
                     /locus_tag="GTK48_RS23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414793.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          2067..2244
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.667"
                     /coverage="82.0"
                     /evalue="2.1000000000000002e-48"
     Typing          complement(1609..2121)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.039"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2355..2671
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.509"
                     /coverage="99.0"
                     /evalue="6.609999999999999e-60"
     Typing          2668..4160
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.4"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttaactgaa atttagagtc tctttttcca ataagaataa ataagaagtt acatgctatg
       61 attagtagcc ctataatttc ttttgttgaa aagaaaaaat tactattaat ctcatctggg
      121 ttagtatcaa gaataatatt aatgatttca tttggcacct gatgaaaata ccaggaggtc
      181 attcgatatg tcattaaatc aacaaataga agtgaaataa taaaacttga tattgataaa
      241 gaaataattg aattaatttt tgaagtgtaa ccatggaaaa aaataacacc aagtatgata
      301 agcgttaagg agtaataaat attactagcg attattctat gggaagtatt aattataaaa
      361 attagattaa taacacataa agctatgtat atgttttttt gatggttttt tgaaatttta
      421 ttgatgatgt tcattatttg ataacataga tataagtaag taagtaagta agtaagtaag
      481 tttaaaaaaa aaatcatagg aaatcattat ttaattaaaa agccgctctc ggtaatgctg
      541 ccaacttact gatttagtgt atgatggtga tttttaggtg cttgcatggc ttccatttcc
      601 atcagatgtc cttcctgctc cgctactgaa ggcgtggtgc gtaacggcaa aagcactgcc
      661 ggacatcagc gctatctctg ctctcattgc tgtaaaacat ggcaactaca gttcacttac
      721 accgcctctc agcccggtac gcaccagaaa atcattgata tggccatgaa tggcgtcgga
      781 tgtcgcgcca gtgcacgcat tatgggcgtt ggcctcaaca cggttttacg tcacttaaaa
      841 aactcaggcc gcagtcggta acctcgcgta tacaaccggg cagtgatgtg attgtctgcg
      901 ctgaaatgga cgaacattgg ggctacgtcg gtgctaaatc acgtcagcgc tggctgtttt
      961 acgcgtatga caggatacgg aggacggttg tggcgcacgt cttcggtgaa cgcactctgg
     1021 ccacactgga gcgtcttctg agcctgctgt cggcctttga ggtcgtggta tggatgacgg
     1081 atggttggcc gctgtatgaa tcacgcctga agggaaagct gcacgttatc agcaagcgtt
     1141 acactcagcg cattgagcga cataatctga atctgagaca acatctggca aggctgggac
     1201 ggaagtcact gtcgttctca aaatcggtgg agctgcatga caaggtcatc gggcattatc
     1261 tgaacataaa acactatcag taagttggag tcattaccaa gaaccgatac gcccctcgcg
     1321 cacttctgct attgaaaaag ccatatttaa gctcactttt tttactctga atataacctg
     1381 ttagagagga ttttcttgag atcgtccgtc tgtcgtcgta gtctcttatc cagaaacgaa
     1441 aaaaccacct ggggaggtgg tttttcgaag gttcagtaag ttggggaact tctgaaccgt
     1501 ggtaacaggg tgtacaagac cgctgccacc aaattcgtcc ttccagttta gccgtagttt
     1561 ggctctaact tcaagaactc tgcatcagta atctcttgta caccctctac cagtggctgc
     1621 tgccagtggc gttaagtcgt gtcttttcgg gttggactca agacgatagt taccggataa
     1681 ggcgcagcgg tcggactgaa cggggggttc gtgcacacag tccagcttgg agcgaactgc
     1741 ctacccggag ctgagtgtca ggcgtggaat gagataaacg cggccataac agcggaatga
     1801 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccatca gggggaaacg
     1861 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt
     1921 gatgctcgtc aggggggcgg agcctatgga aaaacggcag ctccgtatcc tcttcctgtc
     1981 ctgcttgcca tgccgtatgc ctttgctctt atccgtttgc aggtaacgcc cgccgcagcc
     2041 gaacgtcagg agcggaagcg accgagtgag cgaggaggcg tcatatcacc gtaacctcca
     2101 ttttctcctg tacgccctgc agctgcattt ttcccctgcc acatgaagca cttttattaa
     2161 gtgttactcc gccgtaacat attgagccag tatacactcc gctagcgcta cgtgactggt
     2221 tcagggctgc gccccgaaac ccgccaaaag cctgacgcgc ctgcggcttg tccaaccccg
     2281 cgccgaccgg gaaaggcttt cccgcccgtc ccggcgttat caggaggccg gtttgtcaga
     2341 gaaacggaac aagatgctca ccatgtgggt gacggaggac gagcaccggc ggctgcttga
     2401 gcgctgtgac ggcaggccgc tggcggcctg gatgcggcag acctgcctgg acgaaaagcc
     2461 tgcgcgcacc ggaaagctcc cctcaatttc gccggcgctg ctgcgccagc ttgccggcat
     2521 gggcaataac ctgaaccaga tagcccgccg tgttaacacc ggcggcggta ctggccatga
     2581 cagggtacag attgtggcgg cgctgatggc catcgatgcc ggacttgagc ggctgcggca
     2641 cgccgttctt gagaaaggaa cggacgatga tcgttaaatt tcatccacgc ggtcgcggcg
     2701 gtggcgccgg gccggtggac tatctgctgg gtaaagaccg gcagcgtgaa ggcgccaccg
     2761 tcctgcaggg gaagccggaa gaagtccggg agctgattga cgcgtcgcct tacgttaaga
     2821 aatacacctc cggcgtcctg tcgtttgcgg aggccgatct ccctcccggc cagcgggaaa
     2881 aactgatggc cagcttcgag cgggttctga tgcccggact cgataaagat cagtacagca
     2941 ttctgtgggt ggagcacgcg gacaaggggc gtctggagct gaactttctg atccccaata
     3001 ccgagctgct gaccggcagg cggctccagc cttactacga ccgcgccgac cggccgcgca
     3061 tcgatgcctg gcagactatc gtgaacggca gactggggct gcacgacccg aacgcgccgg
     3121 aaaaccgccg ggcgctggtc acgccgtccg ggctgccgaa aacgacgcag gaggccgcag
     3181 aagccattac ccggggacta ctgagcttag cctcgtccgg ggagctgaaa agccgtcagg
     3241 atgtcactga cactcttgaa ggtgcaggtt ttaaggtggt gcgcaccacg aaaagcagta
     3301 tcagcattgc cgacccggac ggggggcgaa acatccgact gaagggagct atctatgagc
     3361 aatcttttaa cgctagcgaa ggacttagag cagcaatcga aagcgcagca gaagagtact
     3421 ggcgagatgc tgaaagccgc attctgcgag cacgagcagt ctgtcagagc ggaactgagc
     3481 gcaagcgcga ggagaatcag cgacgccatc ctcgcccacg agcagagtat gagcgaggcg
     3541 atggagaaaa accggcggag cgtactgcgg actgccgggc ggacatggct caccatcctg
     3601 atggtgtcag cgctgctgat cggcacgagc gggagcatac tgtggtggca ggggcagcag
     3661 ataaccgaca attacacgca tcttcgtcag caggaagata ccctggcgaa aatgaccgcc
     3721 cggacgtggg gagtgcgcta tcaggagagc agcgacggcc ggaggtttct tatcctaccg
     3781 ccggggatga aaaccgaagc catcccttac ggcgggacga catggatccg cctgaaacag
     3841 gagtagcgga agatgacaga gctggaaaag cagttgctga gcgcattaga gcagctacag
     3901 caggactact cgcaaaggct ggacgaatgg gagagcgcct tcgcggaatg gcggagcatg
     3961 tgtgggctta tgcaacggga gaacgcggtg ctgaacgagc gcgtgtcgga cttgagcacg
     4021 caggtgctga gtttaagcga gcagctgcgc cgcttgtccg ggaactgaat gccatcgagc
     4081 tccggcagag ggagaatgag tatcagaaaa cactggagct ggagcgccag ccgcagaaaa
     4141 cctaccgcgg gccgacactt taaaccatcc aggcagctga caacccgcac gaagaggggc
     4201 tcttacttct cccgctaaat aggttcgtga ttaaaaagat cacggcctat acctgggggg
     4261 gaattatgaa attgcttaac gtcattgaag ctgtacttaa ctgcctgtat ctggcggccg
     4321 ggctgtggga ctggctggag aagcatttac ctgtttttaa ggcgttcgcg ctgattttag
     4381 tgtgacttta cacgccatgc atctggctgc atggctatgc agtcgggtaa aatttatctg
     4441 tcacgaacgg ggcggtgtat cgggtggttt gttgccggtt ttacctgact gccgccccgt
     4501 ttgtggcgaa cccgtccgga gcggtgcggg caacagatgt tatggtaaat aacatccgtt
     4561 gttaattaaa cttgaaatca actttaatta taaaaatgaa gctagttaaa ccatcctcca
     4621 gcttcttttt ctctggcttt cccttcgctt ttcattaaat ctttcccgcc ttctgaaata
     4681 tttctgtttg agtcggcgtg agattgaatt acatctgttt gggctgcctt gttttttaac
     4741 tcttggtcta tgaattaatt ttcgtgttta actcttgcct cttccctggc taatgctaac
     4801 ttttgttttt cgatttcaag ctttcttagt tcatcttcat atttctgatc tctattttta
     4861 tcggctttga gctcagcttc gattctttca cggtgagctt ttctctctgc agaagctatg
     4921 gctgctctcc tgttcgcttc tgctcttgct ctttctctcc ttttttgctc aagccgtttg
     4981 ttttcctcaa ttttttctct ctggatatct atgagtcttt gctcttcagc tttcccctgc
     5041 tgttcagctt gagctactgc ggataattgt tcttgaagtg agtttgcaaa gctgcttcca
     5101 ctaaaagaaa ggatgagggg tataatgatt cttttcatcg tttgaatctt atttttttac
     5161 cgggcatgtt gtgtttggct ggattcttgt ttcattattt tttgtggaaa taacaacagc
     5221 taaaccagta gtaaactgac atggttttcc tacttgagtt gatgtaaata cctttgtccc
     5281 ttctttgtaa gttaaagaaa caccttcaac gatagattta tctttgacta atgttcctac
     5341 tgcggcaccg acggcaccac cgccaacggc tccggctgtt gtgcaaagtc cgctaccact
     5401 tccgacgtta tgaccagcca caccgccaac cacagcgcca aggattgccc caaaggtttg
     5461 tgcagcttgt ttgtttgcag tgttatcaac agcaaccttc gcaggcaaaa cagaaattat
     5521 gtttacagtt ttcgtctcct gttttgcatt cagttgccct gtgtcataaa cgtcagcagc
     5581 atattgatcg gcattggatt gacagcctga taaagcggta atcgaggcgg ttagtagggc
     5641 tatgcttaac agtgtttttt tgagctcatg tgacttcctg tttcttaatg taaaaaaaac
     5701 atttttagga attatccgta aaaagtaaag gctaaaggtg gtggttgatt ttccggtaat
     5761 ggtgccaact tactgattta gtgcatgatg gtgtttttga ggtgctccag tggcttccat
     5821 ttccatcaga tgtccttcct ggggtcggtt tgggaaatgg aaattattgt acgttaagaa
     5881 cataatttaa ccgtttctga ttggtaactg attgatttat tgttactgac tatcgcattt
     5941 aagttattgc gctcacgctg tcgcatttag gttgtctcat tacccaaaca tacaggctaa
     6001 atgccgtata gtgcaggagt acaccctatt gcgatagcgc gatctgttca aaaaacaggc
     6061 ttaacgtaca ataatttcca atctccaaac tcacccctta ttcacgctgg ccaacctgtt
     6121 tcgggcggac caaatgatac gtcagtggga gagatctcac taaaaactgg ggataacgcc
     6181 ttaaatggcg aagaaacggt ctaaataggc tgattcaagg catttacggg agaaaaaatc
     6241 ggctcaaata tgaagaaatg aaatgactga gttagccgag aagaatttcc ccgcttattc
     6301 gcaccttccc taactttgca acagtgtctt atattttgta taacttagta aacatattta
     6361 acctgacaga cacctaataa aaataagtaa ctgccaggtt aagttttagc tagttcaatt
     6421 atatggaaat cattttataa atcttggtcg aaatcataaa ctttaccatt tttcaccaat
     6481 cgctgttttt caataaattt atcgtttagt agtgaacaac cttttttaat cttactttca
     6541 ataaaggata actctaaact agtatctacg aaacattcgg atgttatttt tttatcttta
     6601 ttaattgaaa gtttttgtat tagctcagga tgttgtttat ggtataaatc attaaaccaa
     6661 aaaaaatgag caatgttata gttgcctttt gtattaccgc catacaacga tttaccatca
     6721 tcataaatag cagttccatg gtctgaaata aaagataaat atgcaagtcc atctgtattt
     6781 tttaacacat taatgaaatc gcttatctgt ttatcaacag aaaataattc tgaattagca
     6841 tagctatttt ctaatttatc cttatctttt aaagatggag ttgaatatgt tttttcatta
     6901 aaagatggtt tgaaaacatc ataatttgat ggataccttg tatgatacag ataatgactc
     6961 ccgatagttt tataaactat gagctttttc ttgcttttat tatttaatat tttctctaat
     7021 gtattcacgt tgacatcaga tacaacataa tgatcagctt catttgcaat taaatttatt
     7081 tcattatctc ctatgtgact tttactcaga taagaaacat accaagtttc atatccagcc
     7141 tctttataag cactaattat tgatgtttca ttaaaaagac cattatcata cacacctttt
     7201 ctggaaagga tcattggtgt ggaaactaaa gtatatgctc cttgagatat tgtgttttta
     7261 aaagaaacta aattttcttt atctaaattt ggggttgtgt ttttagggta accattaaaa
     7321 tgtaaataat ctcctctatt agtttcgcct aaaaaaagaa ccactgtttg cttatcattt
     7381 attgtagatt tagaatccca cttgaagttt tttttatctt ttataatttg tgcatttttc
     7441 ttaagttcag ataaaccttt attaatttct ttcaaagcta atcctaatgg cttaaaggcg
     7501 attaacaaag aaacaaacag aataatgaaa atataaaaat gc
//
