LOCUS       NZ_CP047384             6988 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli strain CAU16175 plasmid pCAU16175_6, complete
            sequence.
ACCESSION   NZ_CP047384
VERSION     NZ_CP047384.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13705263
            Assembly: GCF_011996085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Guo,L.
  TITLE     The mcr-1 gene is harboured on a Tn2-like cassette in an IncFII
            plasmid from a swine atypical enteropathogenic Escherichia coli
            isolate
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6988)
  AUTHORS   Guo,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2020) College of Veterinary Medicine, China
            Agricultural University, Yuan Ming Yuan West Road 2, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. o.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 02:09:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,274
            CDSs (total)                      :: 6,138
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 136
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 427
            CDSs (without protein)            :: 427
            Pseudo Genes (ambiguous residues) :: 0 of 427
            Pseudo Genes (frameshifted)       :: 160 of 427
            Pseudo Genes (incomplete)         :: 258 of 427
            Pseudo Genes (internal stop)      :: 91 of 427
            Pseudo Genes (multiple problems)  :: 75 of 427
            Pseudo Genes (short protein)      :: 3 of 427
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047384.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CAU16175"
                     /host="swine"
                     /db_xref="taxon:562"
                     /plasmid="pCAU16175_6"
                     /geo_loc_name="China: Hunan"
                     /collection_date="2016"
     gene            join(6545..6988,1..117)
                     /locus_tag="GTP13_RS31035"
     CDS             join(6545..6988,1..117)
                     /locus_tag="GTP13_RS31035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            124..357
                     /locus_tag="GTP13_RS31040"
     CDS             124..357
                     /locus_tag="GTP13_RS31040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(841..1263)
                     /locus_tag="GTP13_RS31045"
     CDS             complement(841..1263)
                     /locus_tag="GTP13_RS31045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1385..1663
                     /locus_tag="GTP13_RS31050"
     CDS             1385..1663
                     /locus_tag="GTP13_RS31050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(1655..1945)
                     /locus_tag="GTP13_RS31055"
     CDS             complement(1655..1945)
                     /locus_tag="GTP13_RS31055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            2185..3831
                     /gene="cka"
                     /locus_tag="GTP13_RS31060"
     CDS             2185..3831
                     /gene="cka"
                     /locus_tag="GTP13_RS31060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_011264160.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(3828..4118)
                     /gene="cki"
                     /locus_tag="GTP13_RS31065"
     CDS             complement(3828..4118)
                     /gene="cki"
                     /locus_tag="GTP13_RS31065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            4148..4279
                     /locus_tag="GTP13_RS31070"
     CDS             4148..4279
                     /locus_tag="GTP13_RS31070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5285..5476)
                     /locus_tag="GTP13_RS31075"
     CDS             complement(5285..5476)
                     /locus_tag="GTP13_RS31075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5544..5867
                     /locus_tag="GTP13_RS31080"
     CDS             5544..5867
                     /locus_tag="GTP13_RS31080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6076..>6513
                     /locus_tag="GTP13_RS31830"
                     /pseudo=""
     CDS             6076..>6513
                     /locus_tag="GTP13_RS31830"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     Typing          5113..5200
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(4546..5102)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.633"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4..464
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.351"
                     /coverage="99.0"
                     /evalue="9.98e-63"
     Typing          5858..6987
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.735"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ggtgtgacgt atcacgaggg caggaacggg aaatttctgg tgttgccgtc cggggtgaag
       61 ggggaaaaca actggacggt ggagaagaag aacgcagtaa gactggtcag ggagtaagtg
      121 agtatgacag agctggaaat gcacttactg aacgccttag agcaattgca gcaggactat
      181 acgcagcggc tgaaagactg ggagagcgcc ttcacggatt tgcagcatat gttcgagctg
      241 acacgacagg agaacgggca actgagggaa cagtgcgacg acttgagtca gcaggtgcag
      301 agattagccg ggcaggtgga ccacttgagt cggttattta tcacgaacag cagataaggg
      361 atgaacagga aaggcaggca cgcctggaac gggagcgtgc gcggtcctgg catgaagagc
      421 aggaacggga agcacgggta tggcgtggcc cgtcaatagg gatgtgacgg tgagttgttc
      481 cgggaggttg ccagatggta ccggaacgcg ttaaaaacgc atccgggcgc gattctggcg
      541 tgtcgttttt gttgctgtgt tttgcctctt tgccagatcg cgctcattcc tcagcctcat
      601 cccgcgatgt ctgcgtatgt catcaactga aaagatggaa aaaaaacagt gtcatgaggt
      661 ttacgcaggc aaaactagcg ttattcacat ggctgaatag ctatgcatac ggttaaaatt
      721 tatcaggtgc gatcgcggca gcttttcggg tggtttgttg ccatttttac ctgtcagcag
      781 ccgtgatcgc gctgaacgca tttcagcggt gcgtacaatt aagggattat ggtaaatccc
      841 ttactgtctg ccctcgtatc catcgagata aaccgcagaa atggtgtctg ccattagttt
      901 catttttttc ggcgaaggca tatcgctgtt tgtgtatttt ctgccagcaa aggctgttgt
      961 gctccggagt gagtgaaaaa atttttctga gtggaattct tttacacgtt gctgtgccgt
     1021 ttctgctgat actccggctg ctttgtcttt catccccata tcccggaact gctgaaagaa
     1081 cgggagcatc atacgtatgg tgtctgcttt ctgggtgtag aagttaggat caaatccatc
     1141 atctgaagca taaacataat gccgggtatg tcgttccagg ctggtctttg gtgtgcatcc
     1201 tgtaagagct attgttgctg ttatcagagg tatcagaagc agcctggcag cggcagatct
     1261 cataaacatc tccttgcgtt tttaatcttg atactcaggg agttatatct ccgaagagta
     1321 tctcttatca tacgtcggtt tactcccaat gtgatttgat ggtcaatgag tcaggaataa
     1381 tcccgtgaaa aaatgtgtgc cagtcctgct ttttagtgtt ctttctgctt cggttgccag
     1441 tcatgcggac agcaatgttt ttcaacaaaa ggccgagaag acaaaaaacg gcattgttgc
     1501 ggatgttaat gccagacaga aaatgttcag taacagactg aagatacaac ttcagatcga
     1561 tgagttgaga gcagagctgg ctggcaccag agatgcccaa aaaagagagg atttgcgtaa
     1621 aaaactggag tctctggagc ggcaaaagaa atccttacgc tgatgggggc gtattttccc
     1681 cggtttcttc cataaccttt ttaccgttca gatatttacg ggcgaggcga atgcgtcttg
     1741 cctggtatac cgccgagtaa gcctgggtgg gattcacacc attactgatt tcactgacca
     1801 catgacgctg atcagccagt gtcagcgtgt aaacctgttt ctggatgagg tcacgggctt
     1861 tgtcatgctc atatccggca gcaacaaggg tgtcgagaag tcgtttgtct gttttgttca
     1921 gtttatggcg gggtgatttg ctcacatcgt tctccgtttt ttcatgatcc aacagtttta
     1981 tatctcatcc tgatttgctc cggtgttctg tttcgacgtt ctcaatgcct gcctccccat
     2041 agtgtgatcc ttattgcaaa aatgaaaatc catgctcttg acatggacaa tgctgagtag
     2101 taggttttac tgtatataaa accagtggtt atatatacag tatattgttt ttaatttatt
     2161 gttttaatta tcaaagagga atttatggct aaagaactaa gtggatatgg accaactgct
     2221 ggtgagtcta tggggggaac aggggcgaat ctgaatcagc aaggtggaaa taataatagt
     2281 aatagtgggg ttcactgggg tggtggctct gggcatggta ataatggtgg tcagggaaat
     2341 tcaaattcat ctggttctac atcaactgtg atgaaaaccg gtgagtcata tctgactccg
     2401 tggggtgatg ttgtcattaa taatgatggc cttccggtga tgaatggtat tgttatgaca
     2461 gaagagaatt caactctggt tgataatcca tttggagggg tctccagagt tcttaattct
     2521 ttaattagtg atatgccatc actttttgca gaaagctcag gaaataacaa caataacact
     2581 gcttcagtta atactgcacc aacgaatgct caggtcagtg atatggataa gagttcgaag
     2641 gtggtgggta atgtaattaa tgaaaaacaa aagcagaaga ataaaattgc tacacagatt
     2701 agtgaaaaac agaagaaaat cgaggagatg aaaaaagtat tcaagcatca ttcttatcat
     2761 ggtatcactg atcttgagag ggatgttgat gagttgcaaa aaaaatcaaa tcagcttgat
     2821 gcagatatta gcaaacttaa ttcttataaa aatacattgc aaagtaaaat tggtgatgtg
     2881 aataaacaga aggaagctga agaaaaggct cgtgaaaatg cagaggtcgc tgaacatgaa
     2941 actcttaatg aagagaaaca ggcggtggcg gaagcagaga agcgtttagc tgaggcgaaa
     3001 gctgaactgg cgaaggctga aagtgatgta cagagtaagc aagctactgt ttccagagtt
     3061 gcaggagagc ttgaaaacgc tcagaagagt gttgacgtga aggtcaccgg atttccggga
     3121 tggcgtgacg ttcagaaaaa actacagcgt cagcttgaag ctaagcaggc tgagtactcc
     3181 gcagttgaaa atgaacttaa gaatgctgtt agtttcaggg atggtaaggc cgctgaggtt
     3241 aaagaggcag agcagaaatt aaaagaagct caggatgctc ttgaaaagag ccagataaaa
     3301 gatgctgttg atactatggt tggattttat caatatataa ccgaacaata tggagaaaaa
     3361 tacgcaaaaa ttgctcagga tttagcggaa aagtctaaag gaaagaaaat tcaaggtgtt
     3421 gatgaagctc tggcagcatt tgaaaagtat aagaatgtac tggataagaa attcagtaag
     3481 gttgatcggg atgctatttt taatgcgtta gagtctgtta attatgatga gttatctaag
     3541 aatttaacga agatatcaaa gtcgcttaaa atcaccagtc gagtatcttt tctatatgat
     3601 gttggttctg attttaaaaa tgcaatagaa actggtaact ggcgaccttt gtttgtcacg
     3661 ctggagaaaa gtgcggtcga tgtaggtgtg gcaaaaattg tggcattaat gtttagtttt
     3721 attgttggtg tgcctcttgg tttctggggg attgcaattg tcacaggtat tgtttcttct
     3781 tacatagggg atgatgagtt gagcaaactt aatgagttgt tagggattta atttctcttt
     3841 agagtgtaat atatgaaact tggcaatcct actgggattg ccattaataa ataaaacaat
     3901 gcagataact tgcataagta ccagggttct ttttttaact ttgtattttc tttgatatat
     3961 ctagatatgg ttaatttaga gtatggatat agcaatacat gtattgatga tataaatata
     4021 aataacaaag gagtgttatc attgtcgtta aatattaaaa taagaatcca tggtataagt
     4081 gattcaggta aattatgtag gtagtatttt aaatgcataa gtatttttac ttttgttgga
     4141 aagaaatatg aaaataataa tttttctaat tacaatatta ttcttgtcag gatgtcaagc
     4201 aaactatatt cgtgatgtac aaggaggaac cgttgcacca tcttcgtctt ctgaactgac
     4261 ggggatcgcg gttcagtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4321 gtaatctgct gcctgtaaac gaaaaaaccg cctggggagg cggtttagtc gaaggttaag
     4381 tcagttgggg actgcttaac cgggtaactg gctttagtgg agcacagata ccaaatgctg
     4441 tccttccagt gtagccgcta taaggcttca acttcaagag ttctgcgtat ctaaatccac
     4501 tatccagtta ccaatggctg ctgccagtgg cgttaagtcg tgtcttaccg ggttggactc
     4561 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     4621 gcccagcttg gagcgaacga cctacaccga actgagatac caacagcgtg agctatgaga
     4681 aagcgccacg cttcccaaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     4741 aacaggagag cgcacgaggg agccaccagg gggaaacgcc tggtatcttt aagtcctgtc
     4801 gggtttcgcc acctctgact tgagcgtcgg tttttgtgat gctcgtcagg ggggcggagc
     4861 ctatggaaaa acgccagctg cgtggccttt tccgggttct tgttttttgc tcacatgttt
     4921 cttttggtgt tatcccctga ttctgtggat aaccgcatta ccgtctttga gtgagctgat
     4981 accgctcgcc gcagccgaac gaccgagcgg agcgagtcag tgagcgagga agcggaaaag
     5041 cgcctggacg agcattttct ccttacgcat ctgtgcggca tttcacaccc ggcatggtgc
     5101 acttttcata caatcctcat tgatgccaaa tgattaagcc agtatacact ccgctatcgc
     5161 tacgtgactg ggtcatggct gcgccccgac acccgctaaa acccgctgac gcgccctgac
     5221 gggcttgtct gctcccggca tccgctcaca ggccagctgt gaccgtctcc gggagctgca
     5281 tgtgtcagag gttttcaccg tcatccccga agcgtgcgag gcagctgcgg taaagctcgt
     5341 cagcgtggtc gtgaagcgct tcgcagatgt cagcctggtc gtcggcgtcc agttcattca
     5401 gtttttccag cagggtcagc gtctggcttc tgatgaagcg ggccatgtta agggcggttt
     5461 tttcctgttt agtcacagtt ctgtttctcc atgtgtgggg agtctgtcgg aggtttttaa
     5521 tgataccggt gaaacgcgaa aggatgctca cgatacgggt cacggatgag gaacatgcgc
     5581 ggctgctggc acgttgtgag ggaaaacaac ttgcgtcatg gatgcgtaag gtctgcctgg
     5641 gggcaccgcc atcgaaaacg tcaggtcttc cgacactggc accgccgctg ttgcgccagt
     5701 tcgcttcggt cggcaataac ctgaatcaga tagcccggaa gataaacagc ggacagtggt
     5761 cggggcatga ccgggttcat gtggtggctg cactgatggc catcggacgt gaactgagtg
     5821 agctgaggga tgaggtccgt aaacagggag aacgggatga tagttaagtt tcatgccagg
     5881 ggaaaaggcg gtggcagtgg tccggttgat tacctgctgg gccgggagcg taaccgggaa
     5941 ggggcgagag ttctgcgggg tgctcccgaa gaggtgcggg aactcatcga tgccacgccg
     6001 tttgcgaaaa aatacacctc gggggttctg tcttttgcag agcagaccct gccaccggga
     6061 gagcgtgaaa gggtgatgga gagctttgag cgggtactga tgcccggtct tgaaaaaaat
     6121 cagtacagca tcctgtgggt ggagcaccag gacaaggggc gtcttgagct gaattttgtc
     6181 atcccgaata tggagctggc gagcggaaaa cggctccagc cctattacga ccgagcagac
     6241 cgtccgcgaa tcaatgcctg gcagacactg gtgaatcacc attacgggct gcacgacccg
     6301 aacgcaccgg agaacaggcg gacacttgtc accccgaata atctgccgaa agcgaaacag
     6361 gaggccgcag aagcgattac gaggggactg gaggctcttt accatgccgg agcgctaaaa
     6421 acgcgtcagg atgtcactga ggcgcttaca gcggcaggtt ttgaggttgt gaggacaacc
     6481 aggagcagta tcagtatcgc cgacccggag ggtgggcgta atctcaggct gaaaggggcg
     6541 ctgtatgagc aatcttttac aaatggggac agacttcgag aagaaactga aagagcgtgc
     6601 cgtctctaca gagaacatgc tgaacagcga gttcaggaag ctcgaagcgt ctgtagacag
     6661 ggaactgaac ttaaacgggc agaaaatcag agacgctatc agcgcacaca cagcagcggt
     6721 gaaagaacaa ctggagacgc tgaacagtgc cgtgaacgcg cagttcagcg ccacagagaa
     6781 cgaactggcc cggcggcagg aagagctgct ctggaagctg gtcaaagggc ggatactgta
     6841 cccgtcgctg acggcgcttt gcgtcacggg cgggattttt ctggcgtcat gggggctgat
     6901 tcagtggcag gaaagccgga ttgcggcgaa catcctggct atcagggacc aggaagagac
     6961 gctggcgaaa ctccgggaaa aaacatgg
//
