LOCUS       NZ_CP047382            10675 bp    DNA     circular CON 13-JUN-2023
DEFINITION  Escherichia coli strain CAU16175 plasmid pCAU16175_4, complete
            sequence.
ACCESSION   NZ_CP047382
VERSION     NZ_CP047382.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13705263
            Assembly: GCF_011996085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10675)
  AUTHORS   Guo,L.
  TITLE     The mcr-1 gene is harboured on a Tn2-like cassette in an IncFII
            plasmid from a swine atypical enteropathogenic Escherichia coli
            isolate
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10675)
  AUTHORS   Guo,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-JAN-2020) College of Veterinary Medicine, China
            Agricultural University, Yuan Ming Yuan West Road 2, Beijing 100193,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. o.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/13/2023 02:09:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,274
            CDSs (total)                      :: 6,138
            Genes (coding)                    :: 5,711
            CDSs (with protein)               :: 5,711
            Genes (RNA)                       :: 136
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 103
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 427
            CDSs (without protein)            :: 427
            Pseudo Genes (ambiguous residues) :: 0 of 427
            Pseudo Genes (frameshifted)       :: 160 of 427
            Pseudo Genes (incomplete)         :: 258 of 427
            Pseudo Genes (internal stop)      :: 91 of 427
            Pseudo Genes (multiple problems)  :: 75 of 427
            Pseudo Genes (short protein)      :: 3 of 427
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047382.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="CAU16175"
                     /host="swine"
                     /db_xref="taxon:562"
                     /plasmid="pCAU16175_4"
                     /geo_loc_name="China: Hunan"
                     /collection_date="2016"
     gene            complement(join(9317..10675,1..1608))
                     /locus_tag="GTP13_RS30935"
     CDS             complement(join(9317..10675,1..1608))
                     /locus_tag="GTP13_RS30935"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_072024409.1"
                     /translation="MSFLDSKSYDTLFGLPELYEDALPRFSLSCEDIAFARTHRRSHNR
                     LGFAIQLALVRDLGRTLRAGEIPPEAVITVVAEQLDIDPAVFTLYAQREETRREHAREI
                     VSALELRSVRSSDYRMLIIAATREAATTEKGLPITRAVINKLKDQKLLAPAPAQLIRLA
                     MAGRAAARRLSYRELIRDLKPSSISALEQLLTERVGEKSILGWIAEAPEGVKLKNLKGL
                     IARLEVLRTAAISDEQRKRIHVNRYGIIARDSRVLHAREIQRMASERCHATLVAFVIEK
                     QAAITDLAIDMFCKLIGSTRRRAEISQKERRLKAAEVLEVVAQDHIKLGRALLAARDGN
                     TDIESVITASLGWEGLIASMEAAASVASSHHCSELDELIGRQKSLRKLGRLIFSAFSFR
                     AFRPDDSVLLAIGYLQAFYHGRKLPKQIPLDFMSHKWRRRVIHGENTDLQAWEVAVFVH
                     LRERLKTGDIWVDGSRAWRSFEDYLLPHHVFGQMRAESRLGLAIPDNFAEWRAERTTTL
                     NAKLKALAKAASENAIPDAELSSRGLSVSPIREEESDKIKALSRKLYMLVPRIRITSLL
                     AEVHSWTSFLDCFTHYRTGEQAEDEAALMAAILADATNAGAERMAQSSRGVTIHQMMVM
                     MDRHLRTETYASATSVLVDAQQEHPFAAIWGDGHVSSSDGQFFPAGGRGEASLEYNAKY
                     GKRPGASIYGFLSNRFASFFSRMIQASESEAPYVLDGLLHNESSVVIHEHATDTAGATE
                     TTFSMFHSFGYRLIPRIRNLGSRRLYVISPSEEYKPLDALIAGKAKMEVIEQHWDEVLR
                     LKASIGAGLVPPSVILKKLAASPRQNRLNLALREMGRIERSIFICDWLLDTRLRRRSHA
                     ILNKGESRHALARAIFLHQLGEFRNRAAETMAHRASGLNLVVNAIILWNTVYLSRAVDY
                     IRSQGIIIPEELLSSVSPLPWGHISLTGDYLWNEIDHPLERYRPLRTNRFNPKNFTFP"
                     /name="Tn3 family transposase"
     gene            complement(1589..2212)
                     /locus_tag="GTP13_RS30940"
     CDS             complement(1589..2212)
                     /locus_tag="GTP13_RS30940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904945.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_077150956.1"
                     /translation="MLIGYMRVSSNDERQSVSLQRDALIAAGVDPRHLHQDHASGARDN
                     RPGLKSCLAELREGDVLIVWKLDRLGRSLSHLLRIIEDLKIRGIAFRSLTESIDTTTPH
                     GSFLFNLFGTLAEYERTLITERVNAGLAAALRRGRKGGRPPAIDAEKVDQILAALEGGA
                     SKASVCRAFSVPRSTLTDTLRRIGWAGPGGRNLTTDQSNVISRL"
                     /name="recombinase family protein"
     gene            2627..2854
                     /locus_tag="GTP13_RS30945"
     CDS             2627..2854
                     /locus_tag="GTP13_RS30945"
                     /inference="COORDINATES: protein motif:HMM:NF015489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_015056439.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(2851..4416)
                     /locus_tag="GTP13_RS30950"
     CDS             complement(2851..4416)
                     /locus_tag="GTP13_RS30950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_106505281.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEALRH
                     NSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEKEQ
                     AETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRLSSGI
                     HARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLRARAGD
                     EMEEKQKQVTASETRLNQISSEINGIQKAISQANNKRSTAVSRIHDAEDNLKIAQTNLL
                     NSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAFEKYKDVL
                     NKKFSKADRDAIFNALESVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGD
                     WKPLFLTLEKKAVDAGVSYVVVLLFSMLAGTTLGIWGIAIITGILCAFIDKNKLNTINE
                     VLGI"
                     /name="colicin-like pore-forming protein"
     gene            4675..5112
                     /locus_tag="GTP13_RS30955"
     CDS             4675..5112
                     /locus_tag="GTP13_RS30955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024189453.1"
                     /translation="MGHCHFMAWHTLQDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRR
                     EFETSELIRAYGELKQDETPERHSAGQAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDTERRRQEAEREQLQNEIAQLRQALEQEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            5193..5612
                     /locus_tag="GTP13_RS30960"
     CDS             5193..5612
                     /locus_tag="GTP13_RS30960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_016247941.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVELHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLAETISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(6173..6406)
                     /locus_tag="GTP13_RS30965"
     CDS             complement(6173..6406)
                     /locus_tag="GTP13_RS30965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_061672798.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6413..6943)
                     /locus_tag="GTP13_RS30970"
     CDS             complement(6413..6943)
                     /locus_tag="GTP13_RS30970"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_167583069.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVRYHEGSNGRFLVLPEGMKAETNWTM
                     ENGKQNALRLVQE"
                     /name="MbeB family mobilization protein"
     gene            complement(<6981..7412)
                     /locus_tag="GTP13_RS31820"
                     /pseudo=""
     CDS             complement(<6981..7412)
                     /locus_tag="GTP13_RS31820"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(7621..7944)
                     /locus_tag="GTP13_RS30980"
     CDS             complement(7621..7944)
                     /locus_tag="GTP13_RS30980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8008..8199
                     /locus_tag="GTP13_RS30985"
     CDS             8008..8199
                     /locus_tag="GTP13_RS30985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8854..9012
                     /locus_tag="GTP13_RS31825"
     CDS             8854..9012
                     /locus_tag="GTP13_RS31825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_250852012.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGNLVSRAMMA
                     QMVQQSS"
                     /name="replication initiation protein"
     gene            complement(9209..>9289)
                     /locus_tag="GTP13_RS30995"
                     /pseudo=""
     CDS             complement(9209..>9289)
                     /locus_tag="GTP13_RS30995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /name="colicin release lysis protein"
     Typing          complement(8288..8372)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.674"
                     /coverage="100.0"
                     /evalue="6.569999999999999e-34"
     Typing          8392..8926
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.022"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6070..7631)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.666"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tgcatcgggt atggcattct ccgaagcagc ttttgccagc gctttcagtt ttgcgttgag
       61 tgtggtggtt cgctcagccc gccattcagc aaagttgtcc ggaatggcaa gtcccagccg
      121 ggattctgcc cgcatctgcc cgaaaacatg gtgaggaaga aggtagtcct caaaactacg
      181 ccaggcccga ctaccatcaa cccatatgtc acctgttttc aggcgttccc gcaaatgaac
      241 gaatacggcg acttcccacg cctgcagatc ggtattttct ccatggatga cacgacgtcg
      301 ccatttatgg gacataaaat cgagcggaat ttgtttgggt aattttcggc catgatagaa
      361 cgcctgcaaa tacccaatcg ccaacaaaac gctatcatcc gggcgaaaag ctctgaaaga
      421 aaaagcgctg aaaatgagtc gtccaagctt acgtaatgat ttttgtcgtc cgataagttc
      481 atccagttca gaacagtgat gggaagatgc caccgaagct gcagcctcca tactggcaat
      541 caaaccttcc cagccgagtg atgctgttat tacagattca atatcagtat taccgtcacg
      601 tgctgccagg agagcccgtc ccaacttaat atgatcctga gctacgactt caagtacttc
      661 agcggctttc agacgacgct ctttctggct tatctctgct ctgcgcctcg tgctgccaat
      721 caatttgcag aacatatcaa ttgccagatc tgtaattgct gcctgctttt caataacaaa
      781 ggctaccagc gtggcatgac agcgttcgga tgccattcgt tgtatttcac gcgcatgtaa
      841 aacgcgagaa tcccgggcaa taataccgta acggtttaca tgaatccgtt tacgttgctc
      901 atcggagatt gccgctgttc gtaatacctc cagcctggca attaacccct taagattctt
      961 gagtttcacg ccttccggcg cttcagctat ccagcctaaa atgctctttt ctccgactcg
     1021 ttcggtaagc aactgctcga gagcgctaat ggatgaaggt ttcagatccc tgatgagttc
     1081 ccgataagaa aggcgacggg cagccgcccg gcccgccatt gccagacgga tgagttgtgc
     1141 cggagcaggg gcaagtaact tctggtcctt cagcttattg ataactgctc tggtgatcgg
     1201 cagtcctttt tcggtggttg ctgcttcgcg tgtagccgcg ataatgagca ttctgtaatc
     1261 gctactccgg acggaacgaa gctccaaagc cgaaactatt tcgcgggcat gttcccggcg
     1321 ggtttcctca cgttgtgcgt aaagtgtaaa aacagcaggg tcaatatcca gttgttccgc
     1381 aacaacggtg ataacagcct ccgggggaat ctctcctgca cgcagtgtac gaccgagatc
     1441 acggacaagt gcaagctgaa tggcaaaacc cagacggtta tgagatcgac gatgagttct
     1501 ggcgaatgca atatcctcgc aggagagcga aaaccgggga agtgcatcct cataaagttc
     1561 aggtaatcca aaaagcgtat cgtacgattt agagtcgaga aatgacattg ctctgatccg
     1621 ttgtcagatt acgcccccca ggtccggccc agccaattcg tcgtagagta tcggtgagcg
     1681 ttgagcgtgg cacactgaat gcgcgacata ctgacgcttt actggcacct ccttccagag
     1741 ctgcgaggat ttgatcaacc ttttcagcat ctatcgccgg tggccttccg ccttttctcc
     1801 cccgccggag agcagcagcc agaccagcat taacgcgttc tgtgataagt gttctttcgt
     1861 actccgcgag cgtgccaaaa aggttaaaga ggaacgagcc gtgtggcgtt gttgtatcaa
     1921 tagattcagt caatgatcgg aaagcaatac cacgaatttt caggtcttca ataattcgga
     1981 gcaaatgtga gagtgaacga ccaagtcgat ccagtttcca gactatcagt acatcacctt
     2041 cgcgaagttc agcgagacag gacttaagtc ccgggcgatt atcacgagca cctgaagcat
     2101 gatcctgatg taaatgccgg ggatccactc cagcagcaat caacgcatca cgctgcaaag
     2161 aaactgactg acgttcatca ttgcttgata cccgcatata accaatcaac atgtacgaca
     2221 aaccttaaaa ttgaagtttc cgtacagttt ctcattccga cagggtttgt cacgcaaaaa
     2281 atcacagatt tatggattca attttcttca actgcaggac ctgacggaaa acaagtgatt
     2341 tccgtcaggg aggaatcgct tagcgggtgt tatcgcagaa attcccacgg aacccctata
     2401 tagttagcct gacatccaac aaggaggttt atagcgaata ttcccacaaa aaatcttttc
     2461 ctcataactc gatccttata aaatgaaaag aatatataac gaggtttaat ttatgagttt
     2521 aagatactac ataaaaaata ttttgtttgg cctatactgc gcacttatat atatatatct
     2581 tataacaaaa aacaacgaag ggtattattt cctagcgtca gataagatgc tatacgcaat
     2641 agtgataagc actattttat gcccatattc aaaatatgct attgaacaca tattttttaa
     2701 gttcataaag aaagattttt tcagaaaaag aaaaaaccta aataatgccc ccgtggcaaa
     2761 attaaaccta tttatgctat acaatctact ttgtttggtc ctagcaatcc catttggatt
     2821 gctaggactg tttatatcaa taaagaataa ttaaataccc aacacctcat ttatagtatt
     2881 aagtttattc ttatcaataa aagcacatag aatgcctgta ataatagcaa tcccccagat
     2941 acctaatgta gttccagcaa gcatactaaa aagtaaaaca acaacataac taactccagc
     3001 atctacagct ttcttctcta atgtaagaaa tagtggcttc cagtcacctg tatccttaat
     3061 ttttaggata tcagatacca catcatatcc aaaagaaaca tgtcccgtaa tcttcaagta
     3121 cttagcaaac tgatctaaat gcttagccca gtcttcatac ttaaccgact ccagtgcatt
     3181 gaaaattgca tcacggtctg ctttgctgaa tttcttattt aaaacatcct tgtatttttc
     3241 aaaagcagct agagcttcat tcacattgct gattttctta cctttagatt tatcagcaag
     3301 ttcctgtgcc atttttgaat atttttcacc atatttttca gatagcgttt gataaaagct
     3361 aactgttgca tccacagcat ccttaatctg cgagttcagg agattagtct gtgctatttt
     3421 caaattatct tcagcatcat gaatacgtga aactgctgta cttcgcttat tattagcctg
     3481 agaaatagcc ttttggattc catttatctc agagctaatc tggttaagac gcgtttctga
     3541 tgctgttacc tgcttttgtt tttcctccat ctcatcacca gctctcgccc gtagtctggt
     3601 ggcctcaaaa aaaggacgac tctgaagcgg atcattcgcc ctaggagata acagtttaac
     3661 cctttcatcc agttctttat atttagcaga tgcctgatcc aactcatttc gttttccgga
     3721 cagcgtattc gtttctgcat cacgggcatg aataccggag cttaaacggg tattgagcgt
     3781 attaatctct tcatccactt tcgccagctc agattgtgca gcagcaagat ttttttgtgc
     3841 cacctccaca gcccgggcct cttcactcaa tgctgccagg cgtttctctt catcctcagc
     3901 cagtttcaat tggcgttctg tttcagcctg ctccttctct atctctttac gtcgttgttc
     3961 tgcttcctga aaagcctttt ccgcagcttc cgcttcttta cgagcttttt cttctgcttt
     4021 tgcaaggcgc aaccgctctg cttctgcctg catcgctgca ttattggcat gagccaggtc
     4081 aataacctcc ggatgagtgg aattatggcg aagcgcctca ttcacaatat ccttcagatg
     4141 ttgagtcagc gcatcccggt ttgcttttgc tttagcctgt gcttctgcgg cagcttttgc
     4201 tcgggcagcc tgttctgcct gcgttttctt caattgagca gtagaccatt tagctgtggc
     4261 atgaatggct gcagaacttt cacttttgct acctccagtt ccaccaccac cgccagagcc
     4321 actcccgtct ggattaccat tcaaaagagt aatgattacc tgccccttat catcataagg
     4381 aacaccatct ttatagtacg ctacagctgt ttccattata aaatcctctt tgacattaaa
     4441 aacaaatagt taaaagcaat atactgtata tataaccact ggttttatat acagcttaaa
     4501 attacgccgc tgcattttcc ctgtcaaccc tgtggatttt catttttgtg aaaacgatca
     4561 aaaaacagcc acccacacca cgtgctattt ccacccgatg ccacaaaaac cagcacaaac
     4621 attaccgttc tccggcctca gtatgtttta ctgaaaccat gatatgagac accaatggga
     4681 cactgtcact tcatggcatg gcacacactc caggacgcac tgaaaatgac aggcagatcg
     4741 cgttcacagt tttaccgtga tatgcgcgca ggccttgtca gttaccgtac cagccaggac
     4801 ggacgacggg agtttgaaac cagtgaactg atccgggcat acggcgaatt aaagcaggat
     4861 gagacaccag aaaggcacag tgcgggacag gcagaaaatc cacatgatca gcagacagaa
     4921 cgcattctcc gggagctgaa tgagctgaaa caatgcctga cgctgatgct tgaggataaa
     4981 caagcccagg atacggagcg ccggcgccag gaagcagaac gggaacagct acagaatgag
     5041 atagcccagc tcaggcaggc gctggagcag gaaaagaaac ggggattctg gtcccggttg
     5101 tttggtcgct gaacgctgtc atggactgat gataaaatag tcttctgata ataactcacc
     5161 gaaaataaat actttaaggt agggagactc tcatgagacg taccggaaac aaactttgtc
     5221 ttatcgccat gataacagca acagtagctc tcacagcctg taccccaaag ggcagcgtgg
     5281 aactacatac ccggcattac gtatatgctt ctgatgacgg ttttgatccc aacttttcca
     5341 cccaaaaagc cgacacaaca cgaatgatgg tgcctttttt ccggcagttc tgggatatgg
     5401 gagcaaagga taaagcgaca ggaaaatcac ggagtgatgt gcagcaacgc attcagcagt
     5461 ttcacagcca ggaattttta aactcactcc ggggcacaac tcaatttgcg ggtactgatt
     5521 accgcagcaa agaccttacc ccgaaaaaat ccaggctgct ggctgagacg atttctgcgg
     5581 tttatctcga tggctatgag ggaagacagt aagtggattt accataatcc cttaattgta
     5641 cgcaccgctg aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa
     5701 ccacccgaaa aactgccgcg atcgcacccg gtaaatttta accacatgca tagctatgca
     5761 gccatgtgaa tcacgcagga attgccagcc agagacagct gaaacggatt tttttcatgc
     5821 tttcggaagc gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg
     5881 cgatctggca atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg
     5941 cgttttaacg cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc
     6001 cgaaccaacc attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc
     6061 tctgattacc atcccagcga agggccatcc agcgtgcgtt cctgtatttc cggctgacgc
     6121 tcccgttcca gggataacgc atgttcgcgc tcctgtatca gccgtgcctc tcttatctcc
     6181 agttctcgct gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct
     6241 gactcaactg catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca
     6301 tcttctgcaa ttccacgaag gcgctctccc attcgctcag ccgctgcata tagtcctgtt
     6361 gcagctgctc taaggcgttc agcaaatgtc tttccagctc cgtcatgctc tgttactcct
     6421 gtaccagcct taaggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca
     6481 tcccctcggg caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg
     6541 tcttcgcgtt cagtttctgc aatgtctctt cctgcttcct gatttcttcc aggttcgtct
     6601 gtatccgtcc gccaagatac cagagcgtcc cgccactcgc ggtaaacagg agaaagacta
     6661 tccccagtaa catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca
     6721 tcatcgcctc tttcacccct tcccggtgtt tttccagcga tttctctgtc gaggctgtga
     6781 acagggctat agcgtctctg attttcgtct cgtttgaggt cacagcctcg ctgacagact
     6841 cgccgagctt cctgaactcg tcgttcagca ttttctctgt agattcggct ctctctttca
     6901 gctttttctc gaactccgcg cccgtctgta aaagattgct cataaaacgc tcctttcagt
     6961 ctgatattcc tcccgccgtt cgggtctgca atgctgatac tgcttcgcgt caccctgacc
     7021 acttcaagcc ccgcctctgt gagcgcctga atcacatcct gacgtccttt tatctctccg
     7081 gcatggtaaa gtgcgtctat accgcgtgta acgctctcag caagtgcctg tttcgtttcc
     7141 ggcaggttat caggaagggt cagcgtccgg cggttctccg gggcgttggg gtcatgcagt
     7201 ccgtaatggt gattcaccag cgtctgccag gcatcaattc tcggcctgtc tgcgcggtcg
     7261 tagtacggct ggagacgttt tccggtctgt agctccatgt tcggaatgac aaaattcagc
     7321 tcaagccgtc ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga
     7381 ccgggcatca gtacacgctc aaagctcgcc atcacttttt cacgtcctcc cggcggcagc
     7441 tccttctccg cgaacgacag aacaccggac gtgtatttct tcgcaaatgg cgtggcatcg
     7501 atgagttccc ggacttcttc cggattaccc tgaagcaccg ttgcgccttc gcggttacgc
     7561 tccctcccca gcaggtaatc aaccggacca ctgccaccac cttttcccct ggcatgaaat
     7621 ttaactatca tcccgcgccc cctgttccct gacggccaga cgcagccggc gcagctcatc
     7681 cccgatggcc atcagtgcgg ccactacctg aacccggtca ccggaagacc actgcccgct
     7741 gttcacctta cgggctgtct gattcaggtt atttccgatg gcggccagct gacgcagtaa
     7801 cggcggtgcc agtgtcggca gtttcccgga acgggcaacc ggctcaccca ggcagacccg
     7861 ccgcatccat accgccagtt gtttaccctc acagcgttca agtaacctgg catgttcatc
     7921 atcagtaacc cgtatcgtga gcatcctctc gcgtttcatc ggtatcatta ccccatgaac
     7981 agaaatcccc cttacacgga ggcatcagtg actaaacagg aaaaaacagc ccttaacatg
     8041 gcgcgcttta tcagaagcca gacattaacg ctgctggaaa agctcaacga actggacgcg
     8101 gatgaacagg cagacatctg tgaatcgctt cacgaccacg ccgatgagct ttaccgcagc
     8161 tgcctcgcac gtttcgggga tgacggtgaa aacctctgac acatgcagct cccggagacg
     8221 gtcacagctt gtctgtgagc ggatgctggg agcagacaag cccgccaggg cgcgtcagcg
     8281 ggttttagcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtac
     8341 actggcttaa ctatgtggca tcagtgcgga ttgtattgat agtgcaccat atgcggtgtg
     8401 aaataccgca cagatgcgta aggagaaaat gcaacttccg acgctcttcc gcttcctcgc
     8461 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtgtctgct cactcaaaaa
     8521 cgataatgcg gttatccaca gaatcagggg ataaggccgg aaagaacatg tgagcaaaaa
     8581 aaggccgcgc aacaggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8641 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8701 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8761 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgttg gtatctcggc
     8821 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8881 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttaacg
     8941 ccactggcag cagccactgg taacttggtt agcagagcga tgatggcgca aatggtgcaa
     9001 cagagttctt gaagtagtgg cccgactacg gctacactgg aaggacagta tttggtatct
     9061 gcgctctgct gaagccagtt acctcggtta agcagttccc caactgactt aaccttcgat
     9121 caaaccgcct ccccaggcgg ttttttcgtt tacaggcagc agattacgcg cagaaaaaaa
     9181 ggatctcaag aagatccttt gatcttttct actgaacaga gatccccgtc aatttagaag
     9241 aagatgatgg agccacagtc cctccctgaa catcacgtat ataggggttc cgtgggcatt
     9301 tctgcgataa cacacgctaa ggaaaagtga agttctttgg attgaagcga ttagtacgaa
     9361 gtggcctgta tcgttcaagg ggatggtcaa tttcgttcca caggtaatct ccagtgagcg
     9421 agatatgacc ccatggtagt ggtgatacac tggacaagag ctcttccggt ataatgatcc
     9481 cctggctgcg aatgtaatca acagcccgac tgagatagac cgtgttccac agaattattg
     9541 cattaacaac aaggttgagg cccgatgccc tgtgagccat tgtctcggca gccctgttcc
     9601 ggaattcgcc cagttggtga agaaaaatag cgcgggcaag tgcatggcgg ctttcgcctt
     9661 tgttgaggat tgcgtgtgat ctgcgacgta accgagtgtc aagcaaccag tcacagataa
     9721 aaattgagcg ttcaatacgt cccatttcac gcagggccag attgagcctg ttttgtctcg
     9781 gagatgctgc cagctttttc agtattaccg atggtggtac cagaccagcg ccgatcgagg
     9841 ccttaagccg cagaacttca tcccagtgct gttctattac ttccattttg gcctttccgg
     9901 cgatcagggc atcaagtggt ttatattcct ctgaggggga aataacgtac aacctgcgac
     9961 tgccgaggtt gcgaatacgg ggtatgagtc tgtaaccaaa agagtgaaac attgaaaatg
    10021 ttgtttcagt cgcaccggct gtatcggtgg catgctcatg aattaccaca gaactttcgt
    10081 tatgaagtaa gccgtcaagt acgtagggag cctcgctttc agaggcctga atcattctgg
    10141 aaaaaaagga tgcaaaacgg ttcgataaaa aaccataaat cgacgcgcca ggtcgtttgc
    10201 cgtatttggc gttatattcg aggctggctt ctcctcgtcc acccgcagga aaaaactgcc
    10261 cgtccgagga tgacacatgc ccatcccccc atatagcagc aaaaggatgt tcctgttgcg
    10321 catcgaccag aactgatgtg gcagacgcat aggtttcagt gcgtaaatga cgatccatca
    10381 ttaccatcat ctggtgaatg gtgacacccc gggaactctg cgccatccgt tctgctcccg
    10441 cattcgtcgc atcggcaagg atcgcagcca taagtgcagc ttcatcttcc gcctgttccc
    10501 cggtgcgata atgggtgaaa caatcaagaa aactggtcca gctatgaact tctgccagca
    10561 aactggtaat tctgatgcgt ggtaccagca tgtagagttt acggctgaga gccttaattt
    10621 tgtcgctctc ttcttcgcga atcggagaca cagacaaccc tctgctggaa agttc
//
