LOCUS       NZ_CP047196            12001 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain Kp36 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP047196
VERSION     NZ_CP047196.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13671318
            Assembly: GCF_009856585.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12001)
  AUTHORS   Tan,D.
  TITLE     Mobile Genetic Element-Mediated Frameshifting in wcaJ Provides Phage
            Resistance in Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12001)
  AUTHORS   Tan,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-DEC-2019) Shanghai Public Health Clinical Center,
            Fudan University, Caolang Road 2901, Jinshan, Shanghai 201508, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC NGS Cell v. 2.3
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12x
            Sequencing Technology             :: PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 04:06:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,038
            CDSs (total)                      :: 5,915
            Genes (coding)                    :: 5,733
            CDSs (with protein)               :: 5,733
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 182
            CDSs (without protein)            :: 182
            Pseudo Genes (ambiguous residues) :: 0 of 182
            Pseudo Genes (frameshifted)       :: 71 of 182
            Pseudo Genes (incomplete)         :: 121 of 182
            Pseudo Genes (internal stop)      :: 23 of 182
            Pseudo Genes (multiple problems)  :: 33 of 182
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047196.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12001
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kp36"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China: Shanghai"
                     /collection_date="2019-01-10"
                     /collected_by="Demeng Tan"
     gene            complement(357..821)
                     /locus_tag="GS419_RS30595"
     CDS             complement(357..821)
                     /locus_tag="GS419_RS30595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227650020.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGWLSPCLSRAGEKPSCARTSMRTQIITCCSRLTCSG"
                     /name="hypothetical protein"
     gene            complement(2304..2452)
                     /locus_tag="GS419_RS29515"
                     /old_locus_tag="GS419_29515"
                     /pseudo=""
     CDS             complement(2304..2452)
                     /locus_tag="GS419_RS29515"
                     /old_locus_tag="GS419_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /name="colicin release lysis protein"
     gene            complement(2537..2794)
                     /locus_tag="GS419_RS29520"
                     /old_locus_tag="GS419_29520"
     CDS             complement(2537..2794)
                     /locus_tag="GS419_RS29520"
                     /old_locus_tag="GS419_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(2804..2944)
                     /locus_tag="GS419_RS30700"
     CDS             complement(2804..2944)
                     /locus_tag="GS419_RS30700"
                     /inference="COORDINATES: protein motif:HMM:NF020569.3"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /protein_id="WP_235356782.1"
                     /translation="MYEWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKY
                     L"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            complement(2878..4488)
                     /locus_tag="GS419_RS29525"
                     /old_locus_tag="GS419_29525"
     CDS             complement(2878..4488)
                     /locus_tag="GS419_RS29525"
                     /old_locus_tag="GS419_29525"
                     /inference="COORDINATES: protein motif:HMM:NF015479.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_264862473.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKFM
                     NGIPSTVSLKGIVPVMANT"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(4593..6076)
                     /gene="ltrA"
                     /locus_tag="GS419_RS29530"
                     /old_locus_tag="GS419_29530"
                     /pseudo=""
     CDS             complement(4593..6076)
                     /gene="ltrA"
                     /locus_tag="GS419_RS29530"
                     /old_locus_tag="GS419_29530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /name="ltrA"
     gene            6721..6966
                     /locus_tag="GS419_RS29535"
                     /old_locus_tag="GS419_29535"
     CDS             6721..6966
                     /locus_tag="GS419_RS29535"
                     /old_locus_tag="GS419_29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7240..7611
                     /gene="tolA"
                     /locus_tag="GS419_RS29540"
                     /old_locus_tag="GS419_29540"
     CDS             7240..7611
                     /gene="tolA"
                     /locus_tag="GS419_RS29540"
                     /old_locus_tag="GS419_29540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            7608..7973
                     /locus_tag="GS419_RS30600"
     CDS             7608..7973
                     /locus_tag="GS419_RS30600"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            8148..8426
                     /locus_tag="GS419_RS29550"
                     /old_locus_tag="GS419_29550"
     CDS             8148..8426
                     /locus_tag="GS419_RS29550"
                     /old_locus_tag="GS419_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            8414..8728
                     /locus_tag="GS419_RS29555"
                     /old_locus_tag="GS419_29555"
     CDS             8414..8728
                     /locus_tag="GS419_RS29555"
                     /old_locus_tag="GS419_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            8892..9320
                     /locus_tag="GS419_RS29560"
                     /old_locus_tag="GS419_29560"
     CDS             8892..9320
                     /locus_tag="GS419_RS29560"
                     /old_locus_tag="GS419_29560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            9346..9525
                     /locus_tag="GS419_RS29565"
                     /old_locus_tag="GS419_29565"
     CDS             9346..9525
                     /locus_tag="GS419_RS29565"
                     /old_locus_tag="GS419_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(9552..10082)
                     /locus_tag="GS419_RS29570"
                     /old_locus_tag="GS419_29570"
     CDS             complement(9552..10082)
                     /locus_tag="GS419_RS29570"
                     /old_locus_tag="GS419_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(10089..10820)
                     /gene="mobC"
                     /locus_tag="GS419_RS29575"
                     /old_locus_tag="GS419_29575"
     CDS             complement(10089..10820)
                     /gene="mobC"
                     /locus_tag="GS419_RS29575"
                     /old_locus_tag="GS419_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(10820..11734)
                     /locus_tag="GS419_RS30605"
     CDS             complement(10820..11734)
                     /locus_tag="GS419_RS30605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_227650018.1"
                     /translation="MADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVK
                     GLITKLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQII
                     IARDEFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLP
                     ADFVKTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDS
                     FIQKEARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDP
                     LAEYLPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(11779..11958)
                     /locus_tag="GS419_RS30610"
     CDS             complement(11779..11958)
                     /locus_tag="GS419_RS30610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227650019.1"
                     /translation="MYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYA
                     LKNLDQYAISTSTP"
                     /name="hypothetical protein"
     BGC             1..9488
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1623..2203
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.945"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10093..10820)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gtggccatcg cgctcttgcc gctcccggca ggccgaggat ctgaatattg gtcttgcgcc
       61 aggtatcacg agggaccagc accggctggc cctgctcatc cttacccaga aacaccgcct
      121 gctgtttccg gcctgctccg tggaaaaatc ggccagcgcg ttaaccggta ccggctcccc
      181 gaaatgcagg tggcggacgt ctgtcatttc tccggtccgc tggctgctgc gccggctctc
      241 ctgattcagt ttcgcgttca ccgcctcgtc agcggccgca aaatgaccat caagcagcag
      301 gaacgccagc agccgcgcca gaaggacgcc ggcgacaagc cctccggcac accaggctat
      361 cccgaacagg ttagccggct gcagcaggta ataatctgcg ttcgcataga ggtccgggcg
      421 caggacggtt tctccccagc tctcgataaa cacggcgaca accagccccc cagaagggcc
      481 agaccggcag cgccggtcag ccagtacgcg tagttccaga catgtcgccg tgccctgacc
      541 atccgcccgg gtgaatccag cgaggccagc accgaaaatg gcacagtccg gttattatcg
      601 cgcttatagc gatcggcata ctcccgccgc gccttatggc tcagccccac aaaacgcatt
      661 cggccggccc agagttcagc cagaccgaaa aacaggccga taaccagaaa aaaaccggcc
      721 agactgtaat actcccggta cagccagctg acccacgggt aaatctgccg ggccagctcc
      781 gggatatgcc actgccaggc aagcgaatcc gtattaaaca tgcaatctct ctccttcaga
      841 cgacgggttt accggcgtga tgcgcagccc ccgctgcaca agccgcggac atgccccaag
      901 gcatggcggc ggcggtcacg tccgtggtga ttgcgttcag ctttcaccgc ggaggccagc
      961 ctctttccgc cccatgcgga aaaggcgcag ccccggagga acaacgagca cgccccagcg
     1021 tgtgagagga aaaggcggca ccccagtgcg gtcttttccg gtgttccggc ccccgtcaga
     1081 gttcgccata gcgagcggct gaccacccgc aaaatcagga gcagctttgc tgcttcgtca
     1141 ggtgcgtcca gcaccgtgga tttttgtggg tggtcaggcg tgagcctgac gggggccggg
     1201 tgaatttctg gcgtggccgg cgtccagccg gacagagcca gaaagagggg aaaagaccgt
     1261 gcgtcggtgg cgtccagcca gcgtaagcaa ggtcttttcc cctgaagtca gccacgcccc
     1321 agcgggatga cttgcatttc tccgcaagga gaaaaaagca acagagatag gttaacgtaa
     1381 aatgttcagc gaaaaaccga catggttcac ctatcctgat aattgatcgt cagggaatag
     1441 aaagacttaa tcaggggaca atatcccaca tcagcgcgtc cggtgaccgt cttgcaggtg
     1501 ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc tccgggcgtg agactgcggc
     1561 gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag gggactaaca
     1621 tgtgaggcaa aacggcaggg gccgcgccgg tggcgttttt ccataggctc cgccctcctg
     1681 ccagagttca cataaacaga cgcttttttc ggtgcatctg tgggagccgt gaggctcaac
     1741 catgaatctg acagtacggg cgaaacccga caggacttaa agatccccac cgtttccggt
     1801 aggtcgctcc ctcttgcgct ctcctgttcc gaccctgccg tttaccggat acctgtccgc
     1861 cgttctccct tgcgggaagt gtggcgcttt ctcatagctc acacactggt atctcggctc
     1921 ggtgtaggtc gttcgctcca agctgggctg taagcaagaa ctccccgttc agcccgactg
     1981 ctgcgcctta tccggtaact gttcacttga gtccaacccg ggaaagcacg caaaacgcca
     2041 ctggcagcag ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgcggt
     2101 tgctcttgaa gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc
     2161 tctgcgaaag ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa
     2221 ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaggat
     2281 ctcaagaaga tcctttgatc tttctactga accgcgatcc ccgtcagttc agaggaggac
     2341 gatgggccac cgttccgccc tgaacatccc ggatataatt tgcctgacat gcggccagaa
     2401 gaaagccagc aacaatcaat ataaaaagaa aaatagtctt tacctttttc atatactttc
     2461 cctgtattta aagactccgg cattcatccc tgaacaccgg aagtcagtat ttcaaatcat
     2521 ccttgataat tttttgttac cagttaccgt cccgataatc aaaagctata taacaattat
     2581 ccacatcagg ttttatttga tttttaaaaa atggctgtag taaaggaacc cattctttct
     2641 tcaaatcaaa ctcaccatta ttaatattat cctttaaagg cattcccaag ctttcaatga
     2701 cagaaccatc gtcaccaaag tctttagaat actcttcccc tttaaactct tcagttttct
     2761 tatcaaacca tgtcaaacgt aatttaagcc ccatatttac ctctcaaaga tattttttaa
     2821 tgttccgttt tggatctggc cctttaacct gcttacccgt tttgggatca aatgcgccta
     2881 ggtgttcgcc atcactggca cgataccctt caagctcacc gtgctgggaa tcccattcat
     2941 aaatttacgt tttttatcgc cataccatcg ggcccgctta ccaccaccac cttgtttagg
     3001 tgttttagga tcacctcgct ttaactctcc aagtcccttt atgtcttctg ttttaggtac
     3061 tggatgataa tcatggccgt agtctttagt ccccttgcga ggcttgttct tttcctcatt
     3121 taacttattt tctgcatcct tcgctttttt ctccttttgt ttccggctct ccatcgcccc
     3181 ggtaagtgca gccttatctc tggataagga ttcattcatt gcgtttaaag atgcctgagc
     3241 cgcttttaat ttctcattct gattctgaat atccttatca agatagcgaa cctgatcctg
     3301 ccacccggag ccatattcat gaggcgggtt ataagtcaca aaatcatctt tatttgcctc
     3361 tgcctctttt acctttttat cggcttcctg aacagcagcc cttgcaccgg ggagcgcctt
     3421 ctctgtcgat gcaatgcggc tgtttaaggt cgcaatattt ttatcttcgg catccagctc
     3481 agctttcgct ttatcatagt ctcttttcag cccctcttcc ggatgagcgg cgtcccatgc
     3541 ctgctggcga cgcttttctt cctcctgacg ctgttttacc tgtgccgggg taagaacatc
     3601 tgtcaccgac acataaaccg gcttctgtcc ggtctctttc gggaaacgaa taacggcctc
     3661 acgggagtta ccaccggctg taaaaccagc cggacgtgaa tcaccttttt cagcaacaat
     3721 gccttttggc ggggctttcg ctgtcggaac acctttcggt acgctcacct gcagagacgg
     3781 gagacccgga atcgacacac tgaatacccc cggacgtttt gtcggtttcg catccaccac
     3841 agggacactc attggccgac ctgcgacaac cgcaatatgc tgccgctcat ccttcaccac
     3901 atccacaacc cgttgtctga cacgaaccgt cgcctggtcc agtggtaaag tacttgccgg
     3961 agtctccgtc accgcatcgg ccggtaatga cgtcataatt tttgacatca ttttcggatc
     4021 atcttttgca atctcagaag gcagcacacc gtagatcgca atcccccaca gaccaaactt
     4081 aaacggcccc ttcagggcag ccagcacatc agcaacggcg gccgacaacg catcgccgga
     4141 aactgataaa gccagtcctt cagcccccgg agtagccagg gcaggcagac cgaaggccat
     4201 cgcagtggcc gaagactgtc cgccaccaga gctaccgcta tgattgccgc tgttaccacc
     4261 attaccaccg ggtttactcc cgccgttacc aaactccccc ggattatagt tatgaatatc
     4321 tccattcggc gtatgcgtcg taccccagcc cggcccgctg tttggatccg taccaccacc
     4381 tgatgaaggg ccacctttac cagacgtacc gttcacattc ccactggcct gaccaccatt
     4441 atgtcccaga cctgaattac ccggacctcg accgtctcca ccactcatac cgttttcctc
     4501 tttgacattt aaaacaataa attaaagcca acatgtcgcg gtagggatac ccgttaccgg
     4561 ataccccccg cacagatccc ggcgtgcgcg atttacgcac cgggctcctg cctcgggtgt
     4621 ctggcggtga accgctccac aggccatgga tgaagaaccc gaacccttgg tagccatgcg
     4681 gctgccagtt tgtttgcttt cgtccaggtc gtatcatcct tctggctcct gcgcctgagc
     4741 gcccggcgcc agaggtttgt tacgtgtgtc ctgaacttct gcatggtggg gaagttgccc
     4801 ggtaccgagt gatagttcag gtatccctga accactctcc tgagccattt tccctgttcg
     4861 gggattgagt aatgccagcg ccttcgcaga ccgtctttga tggctttcag agttgccgtc
     4921 atccgatccc ggcgggtctt tcgtatcagc atgaacctgc cgttgcgatc tttcccgctg
     4981 atgtgcgtga acccgaggaa gttgaacgtt tctggtttgc cttttcccct gatggcacgg
     5041 ttttcggcag cgaagcggcc gaactccatc agacgggttt tctccgggtg aaccgtgagt
     5101 ccgaactccc tcagtctgcg ctgcatggct atacggaagc gccgggcatc gtatcgtttg
     5161 tcgaacccga tgacgatgtc atcggcgtat ctgaccatta ccacattgcc tgtggcatag
     5221 cgacgtcgcc actgatgcgc ccacagatcg aagacgtagt ggaggtatat gtttgccagc
     5281 agcggtgaga tgaccgcacc ctgtggggtg ccttcctccg ttgctcgcca ttgaccctcc
     5341 tccgacgtcc cggctgtgag ccacttacgt atgagcctga ttaccctccg gtcgccgatc
     5401 cgatgctctg tgaacctgat cagccattcg tggctcaccc tgtcgaagaa ctgactgatg
     5461 tcggcatcca gtacccagtt tacgttagtg cgtaccagcc tgtggccagt gcgtccagtg
     5521 catcgtgctg gcttcgcccg ggtctgaacc cgtatgagaa cccataaagt cgttttcata
     5581 gactgcgttc aggattttca ccagcgcata ctggacgatc ttgtcctcca gcgaggcgat
     5641 gccgagcggg cgttgttttc catccgcttt gggatgtagt gacgcctgcc gggctgcgcc
     5701 ctgtagctgc cctgatgtag cctccggtgc agatctgtta tgttgttctt catgtttccg
     5761 gcgtagtcca tccacctgat gccatccact ccggcggccg ctttcctgct cagggagagg
     5821 aatgcggctt ccagtgcttc gactgtcagc aggtggaaca atgctgtaaa ccgttctttc
     5881 ttccgctgct tcgcagcttc ccgcacgcgt gacagcctct gtgacatgct ttcccggctc
     5941 tgtgtccggc gcattgtggc tgttccgcgt tccccttggc cccgctcctt cgctccactg
     6001 actccgctcc tttcgggttg ttcgcctgct tcgccgctac tatgagcgag tccgacttct
     6061 cctctccgta catcaccggc tatgactcct cgtcttcccg gtgcgggcca tctccgacac
     6121 tggcagatgg tcagagggga gatctcccgg ttcccgcgta gagatcgtat tgacatgcca
     6181 gggtctcaga ccccgccggg tccatgtggc actcgcagtg tcgcacccta tgatgttgcc
     6241 ttccgttaac agtacaacgt cggcacccgg taatttaata tacatttcgt ggctcaatgg
     6301 ctggcctgtc aacacccctg tcaacgcttc gccccatacc tcgcggtatg caacgcatga
     6361 ctcggggacc ttgtggattg ctggtccttc aatggtcggg gactttcacc ccttgatctc
     6421 taaccggtct cccggcgcac actgtacata tacacagtat aattattacc ttactcctcc
     6481 cgttttcagg tgtcaatata tgagattagt tctcatcttc aggcacaaaa aagcgcccgg
     6541 agccacctgt aagcagatcg cgtcttttcc cgaaaatttc tcatggcaac aaattttatt
     6601 atttaagaca ttcatcacac ttacatttca gatggtatag acacatctga aagctaactg
     6661 gcatgaggag aggacctgcg gttaaactgt tcgggaaacc tgagacgccc ggaggccact
     6721 atgacgcacc tgaaactgac tcccgctgaa gaaaagcgcc tggcggacgc ctttgtcgct
     6781 caggcactgg ccatgggcct gaaagggaac aaaacgctgc caaaggcggt aaaacaacag
     6841 gtgcgggaaa gggtgcttcg tgccattcag gacgaacgtg aagccaggcg ggcgcaggaa
     6901 atgcgtcggg agaaatacga ggctacggaa accaccttca cctggcagcc acagcgccgc
     6961 cggtaagggg tatttaccat aacgtcgcat tgcccgcacc gctcagacgg tctgagggcg
     7021 tttccggcgg ctacgggata aaacaggcaa caaaccaccc ggaaaaccgc cggaaacgcg
     7081 cccggtaaat tgtaacccgc tgcatgccta tgcacgcaac tgcatggcgg gccgtttggt
     7141 gtaaaaacga tacgataagc cgtgtttatg taaatgaacg tggccgtaaa gagtgacaaa
     7201 tatacatgta acatttttta ctgacaggtc atcaacccga tgcgtttaat accctttatc
     7261 ctgctggcat tgtgcagccc cttactcctg acggcctgta agcattccac cacccagccg
     7321 ccgaaaattt atactctgga taagtcatct aaaatcagta gctacgctgc ggccatcaag
     7381 accgctgttg aaatgcagat gtttgaacca gaggcctatg cgggaaaaat gtgcaccgtg
     7441 cggatatcca tacaacgtga tggcactctg aacagcgcca ttgctgaagg aggagatccg
     7501 aaactctgta aagcagcaat atcagccgtt acccacgcca aaattccgcc ggcaccggat
     7561 gaaaatacct ggcaaatatt caaaaacgcc cctctggatt tcagaccatg aaagctttta
     7621 ctctgacagt gctggcggcc atgttcctca ctccggtaca tgcgatacca ctaaaacccg
     7681 taaccacact gcagccaacc tacccggtac aggccgcagc aaaacgcatc gaaggtcagg
     7741 tgaccgccac gtttgatatt ctggacgatg gacgagtgga caacgtgtcg cttaccggct
     7801 gtcacctttt ttttcgggaa acgctgacgg caatgaggca ctggctatat cccgcagggc
     7861 acccggcaat gcgacaggtt ataaattttg aattcagtct gaaaaatgac ggcttacagg
     7921 atccgcgacc agtcaccacc caaaagctca atgatgatgg ttgccgccag taacaggtcc
     7981 tttaacccgc aggctgcaat agtcctggat gatgagtgag cctgtagcag ccctgattag
     8041 tggaagcgaa gctgaaaaat gggcagcaaa agccgtattt ctggcaaata gcgccgccac
     8101 ggattaaagt agtcatgtgt aatacatatg actacaaagg ggcttttatg tcatcagcaa
     8161 catccatcag gctcgatgaa gaacttaagg accggctgaa aacgctggcc gatgaccggc
     8221 accgctcagc gcacgcgcta atgctggagg caatcaccga atacatcgac cgggaagaga
     8281 agcgcagcca gtacctgcgg gacggacagg ccgcatggca gcactatcag gaaacgggtc
     8341 tgcaccttac tgcggaagaa gcggaagcct ggataagtac ctggggaact gagaacgagc
     8401 aggatgctcc gccatgccac aggtaacgat ttccgcgctg gcacagcgcg atttacaacg
     8461 ccttcaggac tttctcaaaa ccaaaaatcg gctggcagcc agaaaaggag gtgaggtgat
     8521 cgtccgggct atccagcaac tgaaaacatt gccagacatt ggccgcccgg tgccgtttct
     8581 gccgctggaa tataaggaac tggtgatcgg gtttggcgac agtggctatg tgatgctcta
     8641 ccgccacgac cgggaaatgg accagattgt gatcgtcacc gtcaggcatc agaaagaatc
     8701 cgggtatccg ggggcagaca gcctctaata accgatgacg ggggcgcaat tcattgcgtg
     8761 gccgtcgccg gtttgacaag cgcagcgcgt cagaaaacca catcctgaac ggaaaaacgg
     8821 gtaaaattca gcgatacatt taccgtgaga aattacacac ctttcacacc cgataaaatc
     8881 aggggtatac agtggcgaaa acattgctca acctttcaca ggcagcacaa gccgcgggca
     8941 ttaccagaag aacgctatac aatcatgtaa aacagggaaa ggttaccgtg tcacgtgacg
     9001 ggaaaaacaa ccctgtcgtg gatgtgtcag aactgatccg tgtatacgga aatgtgaata
     9061 tccctgagaa acaaattccc ggcatttcac accgtgaaaa tacacaaaaa aatttcccac
     9121 aggaacagct tctggcaatg caaaaggaac tggccgatct ccggcaggct gtcaccctga
     9181 tgctggaaga taaaacatcc cgggaagaag aacgccgcca gcacgatgat gagcgccgga
     9241 aactccaggc cgaagtggac agattgacaa cagaactgac acagaagaaa aaacgcttct
     9301 ggtccggctg gtttagctga gaacttaccg aagggaatgg agaacatgac gaagcaggaa
     9361 aaaactgccc tcaatatggc taaattcatt aaggatcagt cgctgctgtt gctggaaaag
     9421 ctgaacgagc tggacatgga taccccggcc gacatgtgcg aacggcttca cgaggatgcg
     9481 gaaacactct ggcaggtgat gcgggataag ctcggggaag aataacagca ccggccatgc
     9541 cggtgctggc gttatttttt gccgaacggc caccaccggc gggcgggttt gtcctccggc
     9601 agttcaggca ccggcacgtt aaaatagcgg ctgttatatt cccgcatcag ctccgctttc
     9661 agcttctcgt tcaccgcgct ttccccgtgc cgctcgctga ccacctcaat catcgcgtta
     9721 atctgctccg gtgagcactt cccggcatca accacctgca caccgttctc gatggcgaac
     9781 cgctccacct ttcgatagag gtaatcacac agcttcattc ctgtgggttt gtcccgttcc
     9841 cattgcggat tttcaccggt gaaaaagtac gtggcttttt cgtcgctgtt gagccgctcg
     9901 tcatacatcc cgatggattt catcatcgcc agatgctggg cccggtcgac aaaacagaaa
     9961 acatacgccc cggtctgcca ggcgagcacc accatgtctt ccagatgctc gctggttggc
    10021 ataatgcagc gctcgccgtc tttgtcttcc acgtcctcaa taaataccat gtacagcgcc
    10081 ataatccctc actccgttgt cataaattta aagcacgcga acagcctctc ccggtccagc
    10141 tccaccgttt tgcgggcctc gccggtctgt ggattcacga tttcatccac cgccagaatc
    10201 gcccgctgta cccggacaag cgtcggccgg tcagggcaga tgtaccagat ttcatcgatg
    10261 ccgttcgccc ggcggttgaa cagatacccg gacaccactt cctgataccg cctgcggctc
    10321 ttgacggtgc gctctacctc aaatgccacc gttctgccgt ccagctccgc cagcgcgtcc
    10381 ggcactttca tccctttcag ggccatacgg tgcaggtacc gccagccggt ggccccccgg
    10441 ccctccatgg agaggcgcag ggactgcacg gccagccggt gcggcagctg cgccgccgac
    10501 acccttgacg gctgaaacgg aataacgtcc gtcagcggct cgtcctcgtc gaacgagaac
    10561 gccaccccgt gcggcgtcag cccccacacg ctgacctgac ggccgtcaac cgggtatttc
    10621 gcccggacca gcagcccttt cgccaccaga ctgtccagga agcggtaaac cggctgtacc
    10681 gccgcaatcc ccagccgtcg cgcaaccagc tccgccgtgg aacacagctc ttcccgcagc
    10741 cagcgcaggg tcagccgtgt tttctccgcg ttccgcgcct gccgcttcgc gtggctgacg
    10801 ttgtactgct ccagtgtcat cagtaaagcc ccgcaaaatc atcatccgct tttggtccgc
    10861 cgccgtcggc cggaggcagg tactccgcca gcgggtccgt tttcgccagc accgggaagg
    10921 ttttcagcga aatttcccgg cgctcaaccc gcagcgggct gctgaacgcc agccgcgcca
    10981 cgcccagccc ggtcatcacc gcgaacccgt caggcaggtg ttgcagggtg ttcacgtcaa
    11041 acagcggccg cgcctccttc tggatgaagc tgtcgccgct gatgtgctcc acgttccccg
    11101 cctcccggct ggcccggcgg cgctccacgt ccacccggat ctccccggtc tggcccgccg
    11161 cccactgcgc ggactcctgg ctggccgcgc gatagaacca gcggatcggc gtgttatcca
    11221 gtaccgtggt tttcacaaaa tcagcgggca gatcctgccc gcactgaccg aagtcgccca
    11281 gcgactggtg cgcgagcagc aggttgcagt cccggctgcg cagcgtcccc agcgcgttca
    11341 gcacgtactt cgacagcaga tatttaatct catcgagcat gatgctcgcg tgcggccacc
    11401 tccggaactc atcccgcgca atgatgattt gcgcgcagcg ggcaaacagc atcttctgca
    11461 cccggatcac cgcctcatcg tccatcgagc cgatgacgta caggcagccg ccgccgttga
    11521 ttatccccgc cacatcgata ccgctgtccg tctgcaggac gctcaggtcg gccacgttct
    11581 ccagcttggt gatcagcccc ttcacgtcct ttttcagggc ctccggcaac gcgtacgccg
    11641 ccgccagcac ctgctgaatg tttgccccct gcgggaactg ctccgcaatc agcttcgccg
    11701 ctttctgctc gctgatgcgg tagtggtccg ccacgtcgcc ggtctcactc aggttaaacc
    11761 cggcaaccag cagattcttc agggcgtact ggtcgagatc gcgtactggt cgagattctt
    11821 cagggcgtac tggtcgagat cgcggaacag attcagctgc gccggtttcc ccggccgcag
    11881 atccagcagg gtgaagtccg ggcagtgcgc ccggaagacg tgcggcgccc acgcgtcccc
    11941 tttggggtcg aagtacacca cagcgtcccc gaaatcgcgt acgcagcgaa tgagcgcgtt
    12001 g
//
