LOCUS       NZ_CP047162            10060 bp    DNA     circular CON 28-NOV-2023
DEFINITION  Klebsiella pneumoniae strain KP19-2029 plasmid pKP19-2029-Col,
            complete sequence.
ACCESSION   NZ_CP047162
VERSION     NZ_CP047162.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13663554
            Assembly: GCF_009833945.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Klebsiella pneumoniae KP19-2029, complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Qin,S. and Dong,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2019) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: CLC Genomics Workbench v. 10.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Illumina HiSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/28/2023 03:47:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,673
            CDSs (total)                      :: 5,549
            Genes (coding)                    :: 5,385
            CDSs (with protein)               :: 5,385
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 164
            CDSs (without protein)            :: 164
            Pseudo Genes (ambiguous residues) :: 0 of 164
            Pseudo Genes (frameshifted)       :: 70 of 164
            Pseudo Genes (incomplete)         :: 99 of 164
            Pseudo Genes (internal stop)      :: 14 of 164
            Pseudo Genes (multiple problems)  :: 19 of 164
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047162.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP19-2029"
                     /isolation_source="blood"
                     /host="female"
                     /db_xref="taxon:573"
                     /plasmid="pKP19-2029-Col"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2019-01-04"
                     /collected_by="Zhengzhou University"
     gene            complement(751..900)
                     /locus_tag="GSU00_RS27760"
                     /old_locus_tag="GSU00_27760"
     CDS             complement(751..900)
                     /locus_tag="GSU00_RS27760"
                     /old_locus_tag="GSU00_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(985..1242)
                     /locus_tag="GSU00_RS27765"
                     /old_locus_tag="GSU00_27765"
     CDS             complement(985..1242)
                     /locus_tag="GSU00_RS27765"
                     /old_locus_tag="GSU00_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(1252..2937)
                     /locus_tag="GSU00_RS27770"
                     /old_locus_tag="GSU00_27770"
     CDS             complement(1252..2937)
                     /locus_tag="GSU00_RS27770"
                     /old_locus_tag="GSU00_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3264..3509
                     /locus_tag="GSU00_RS27775"
                     /old_locus_tag="GSU00_27775"
     CDS             3264..3509
                     /locus_tag="GSU00_RS27775"
                     /old_locus_tag="GSU00_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3783..4154
                     /gene="tolA"
                     /locus_tag="GSU00_RS27780"
                     /old_locus_tag="GSU00_27780"
     CDS             3783..4154
                     /gene="tolA"
                     /locus_tag="GSU00_RS27780"
                     /old_locus_tag="GSU00_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            4151..4516
                     /locus_tag="GSU00_RS28795"
     CDS             4151..4516
                     /locus_tag="GSU00_RS28795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            4691..4969
                     /locus_tag="GSU00_RS27790"
                     /old_locus_tag="GSU00_27790"
     CDS             4691..4969
                     /locus_tag="GSU00_RS27790"
                     /old_locus_tag="GSU00_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            4957..5271
                     /locus_tag="GSU00_RS27795"
                     /old_locus_tag="GSU00_27795"
     CDS             4957..5271
                     /locus_tag="GSU00_RS27795"
                     /old_locus_tag="GSU00_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5435..5863
                     /locus_tag="GSU00_RS27800"
                     /old_locus_tag="GSU00_27800"
     CDS             5435..5863
                     /locus_tag="GSU00_RS27800"
                     /old_locus_tag="GSU00_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            5889..6068
                     /locus_tag="GSU00_RS27805"
                     /old_locus_tag="GSU00_27805"
     CDS             5889..6068
                     /locus_tag="GSU00_RS27805"
                     /old_locus_tag="GSU00_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6095..6625)
                     /locus_tag="GSU00_RS27810"
                     /old_locus_tag="GSU00_27810"
     CDS             complement(6095..6625)
                     /locus_tag="GSU00_RS27810"
                     /old_locus_tag="GSU00_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6632..7363)
                     /gene="mobC"
                     /locus_tag="GSU00_RS27815"
                     /old_locus_tag="GSU00_27815"
     CDS             complement(6632..7363)
                     /gene="mobC"
                     /locus_tag="GSU00_RS27815"
                     /old_locus_tag="GSU00_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(7363..9327)
                     /locus_tag="GSU00_RS27820"
                     /old_locus_tag="GSU00_27820"
     CDS             complement(7363..9327)
                     /locus_tag="GSU00_RS27820"
                     /old_locus_tag="GSU00_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..7937
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          70..648
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6636..7363)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ctgcggcggg cgctgcggac acatacaaag ttacccacag attccgtgga taagcagggg
       61 actaacatgt gaggcaaaac ggcagggccg cgccggtggc gtttttccat aggctccgcc
      121 ctcctgccag agttcacata aacagacgct ttttcggtgc atctgtggga gccgtgaggc
      181 tcaaccatga atctgacagt acgggcgaaa cccgacagga cttaaagatc cccaccgttt
      241 ccggtaggtc gctccctctt gcgctctcct gttccgaccc tgccgtttac cggatacctg
      301 tccgccgttc tcccttgcgg gaagtgtggc gctttctcat agctcacaca ctggtatctc
      361 ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc aagaactccc cgttcagccc
      421 gactgctgcg ccttatccgg taactgttca cttgagtcca acccgggaaa gcacgcaaaa
      481 cgccactggc agcagccatt ggtaactggg agttcgcaga ggatttgttt agctaaacac
      541 gcggttgctc ttgaagtgtg cgccaaagtc cggctacact ggaaggacag atttggttgc
      601 tgtgctctgc gaaagccagt taccacggtt aagcagttcc ccaactgact taaccttcga
      661 tcaaaccacc tccccaggtg gttttttcgt ttacagggca aaagattacg cgcagaaaaa
      721 aaggatctca agaagatcct ttgatctttt ctactgaacc gcgatccccg tcagttcaga
      781 ggaggacgat ggtgccaccg ttccgccctg aacatcccgg atataatttg cctgacatgc
      841 ggccagaaga aagccagcaa caatcaatat aaaaagaaaa atagtcttta cctttttcat
      901 atactttccc tgtatttaaa gactccggca ttcatccctg aacaccggaa gtcagtattt
      961 caaatcatcc ttgataattt tttgttacca gttaccgtcc cgataatcaa aagctatata
     1021 acaattatcc acatcaggtt ttatttgatt tttaaaaaat ggctgtagta aaggaaccca
     1081 ttctttcttc aaatcaaact caccattatt aatattatcc tttaaaggca ttcccaagct
     1141 ttcaatgaca gaaccatcgt caccaaagtc tttagaatac tcttcccctt taaactcttc
     1201 agttttctta tcaaaccatg tcaaacgtaa tttaagcccc atatttacct ctcaaagata
     1261 ttttttaatg ttccgttttg gatctggccc tttaacctgc ttacccgttt tgggatcaaa
     1321 tgcgcctagg tgttcgccat cactggcacg atacccttca agctcaccgt gctgggaatc
     1381 ccattcataa attttacgtt ttttatcgcc ataccatcgg gcccgcttac caccaccacc
     1441 ttgtttaggt gttttaggat cacctcgctt taactctcca agtcccttta tgtcttctgt
     1501 tttaggtact ggatgataat catggccgta gtctttagtc cccttgcgag gcttgttctt
     1561 ttcctcattt aacttatttt ctgcatcctt cgcttttttc tccttttgtt tccggctctc
     1621 catcgccccg gtaagtgcag ccttatctct ggataaggat tcattcattg cgtttaaaga
     1681 tgcctgagcc gcttttaatt tctcattctg attctgaata tccttatcaa gatagcgaac
     1741 ctgatcctgc cacccggagc catattcatg aggcgggtta taagtcacaa aatcatcttt
     1801 atttgcctct gcctctttta cctttttatc ggcttcctga acagcagccc ttgcaccggg
     1861 gagcgccttc tctgtcgatg caatgcggct gtttaaggtc gcaatatttt tatcttcggc
     1921 atccagctca gctttcgctt tatcatagtc tcttttcagc ccctcttccg gatgagcggc
     1981 gtcccatgcc tgctggcgac gcttttcttc ctcctgacgc tgttttacct gtgccggggt
     2041 aagaacatct gtcaccgaca cataaaccgg cttctgtccg gtctctttcg ggaaacgaat
     2101 aacggcctca cgggagttac caccggctgt aaaaccagcc ggacgtgaat cacctttttc
     2161 agcaacaatg ccttttggcg gggctttcgc tgtcggaaca cctttcggta cgctcacctg
     2221 cagagacggg agacccggaa tcgacacact gaataccccc ggacgttttg tcggtttcgc
     2281 atccaccaca gggacactca ttggccgacc tgcgacaacc gcaatatgct gccgctcatc
     2341 cttcaccaca tccacaaccc gttgtctgac acgaaccgtc gcctggtcca gtggtaaagt
     2401 acttgccgga gtctccgtca ccgcatcggc cggtaatgac gtcataattt ttgacatcat
     2461 tttcggatca tcttttgcaa tctcagaagg cagcacaccg tagatcgcaa tcccccacag
     2521 accaaactta aacggcccct tcagggcagc cagcacatca gcaacggcgg ccgacaacgc
     2581 atcgccggaa actgataaag ccagtccttc agcccccgga gtagccaggg caggcagacc
     2641 gaaggccatc gcagtggccg aagactgtcc gccaccagag ctaccgctat gattgccgct
     2701 gttaccacca ttaccaccgg gtttactccc gccgttacca aactcccccg gattatagtt
     2761 atgaatatct ccattcggcg tatgcgtcgt accccagccc ggcccgctgt ttggatccgt
     2821 accaccacct gatgaagggc cacctttacc agacgtaccg ttcacattcc cactggcctg
     2881 accaccatta tgtcccagac ctgaattacc cggacctcga ccgtctccac cactcatacc
     2941 gttttcctct ttgacattta aaacaataaa ttaaagccaa catactgtac atatacacag
     3001 tataattatt accttactcc tcccgttttc aggtgtcaat atatgagatt agttctcatc
     3061 ttcaggcaca aaaaagcgcc cggagccacc tgtaagcaga tcgcgtcttt tcccgaaaat
     3121 ttctcatggc aacaaatttt attatttaag acattcatca cacttacatt tcagatggta
     3181 tagacacatc tgaaagctaa ctggcatgag gagaggacct gcggttaaac tgttcgggaa
     3241 acctgagacg cccggaggcc actatgacgc acctgaaact gactcccgct gaagaaaagc
     3301 gcctggcgga cgcctttgtc gctcaggcac tggccatggg cctgaaaggg aacaaaacgc
     3361 tgccaaaggc ggtaaaacaa caggtgcggg aaagggtgct tcgtgccatt caggacgaac
     3421 gtgaagccag gcgggcgcag gaaatgcgtc gggagaaata cgaggctacg gaaaccacct
     3481 tcacctggca gccacagcgc cgccggtaag gggtatttac cataacgtcg cattgcccgc
     3541 accgctcaga cggtctgagg gcgtttccgg cggctacggg ataaaacagg caacaaacca
     3601 cccggaaaac cgccggaaac gcgcccggta aattgtaacc cgctgcatgc ctatgcacgc
     3661 aactgcatgg cgggccgttt ggtgtaaaaa cgatacgata agccgtgttt atgtaaatga
     3721 acgtggccgt aaagagtgac aaatatacat gtaacatttt ttactgacag gtcatcaacc
     3781 cgatgcgttt aatacccttt atcctgctgg cattgtgcag ccccttactc ctgacggcct
     3841 gtaagcattc caccacccag ccgccgaaaa tttatactct ggataagtca tctaaaatca
     3901 gtagctacgc tgcggccatc aagaccgctg ttgaaatgca gatgtttgaa ccagaggcct
     3961 atgcgggaaa aatgtgcacc gtgcggatat ccatacaacg tgatggcact ctgaacagcg
     4021 ccattgctga aggaggagat ccgaaactct gtaaagcagc aatatcagcc gttacccacg
     4081 ccaaaattcc gccggcaccg gatgaaaata cctggcaaat attcaaaaac gcccctctgg
     4141 atttcagacc atgaaagctt ttactctgac agtgctggcg gccatgttcc tcactccggt
     4201 acatgcgata ccactaaaac ccgtaaccac actgcagcca acctacccgg tacaggccgc
     4261 agcaaaacgc atcgaaggtc aggtgaccgc cacgtttgat attctggacg atggacgagt
     4321 ggacaacgtg tcgcttaccg gctgtcacct tttttttcgg gaaacgctga cggcaatgag
     4381 gcactggcta tatcccgcag ggcacccggc aatgcgacag gttataaatt ttgaattcag
     4441 tctgaaaaat gacggcttac aggatccgcg accagtcacc acccaaaagc tcaatgatga
     4501 tggttgccgc cagtaacagg tcctttaacc cgcaggctgc aatagtcctg gatgatgagt
     4561 gagcctgtag cagccctgat tagtggaagc gaagctgaaa aatgggcagc aaaagccgta
     4621 tttctggcaa atagcgccgc cacggattaa agtagtcatg tgtaatacat atgactacaa
     4681 aggggctttt atgtcatcag caacatccat caggctcgat gaagaactta aggaccggct
     4741 gaaaacgctg gccgatgacc ggcaccgctc agcgcacgcg ctaatgctgg aggcaatcac
     4801 cgaatacatc gaccgggaag agaagcgcag ccagtacctg cgggacggac aggccgcatg
     4861 gcagcactat caggaaacgg gtctgcacct tactgcggaa gaagcggaag cctggataag
     4921 tacctgggga actgagaacg agcaggatgc tccgccatgc cacaggtaac gatttccgcg
     4981 ctggcacagc gcgatttaca acgccttcag gactttctca aaaccaaaaa tcggctggca
     5041 gccagaaaag gaggtgaggt gatcgtccgg gctatccagc aactgaaaac attgccagac
     5101 attggccgcc cggtgccgtt tctgccgctg gaatataagg aactggtgat cgggtttggc
     5161 gacagtggct atgtgatgct ctaccgccac gaccgggaaa tggaccagat tgtgatcgtc
     5221 accgtcaggc atcagaaaga atccgggtat ccgggggcag acagcctcta ataaccgatg
     5281 acgggggcgc aattcattgc gtggccgtcg ccggtttgac aagcgcagcg cgtcagaaaa
     5341 ccacatcctg aacggaaaaa cgggtaaaat tcagcgatac atttaccgtg agaaattaca
     5401 cacctttcac acccgataaa atcaggggta tacagtggcg aaaacattgc tcaacctttc
     5461 acaggcagca caagccgcgg gcattaccag aagaacgcta tacaatcatg taaaacaggg
     5521 aaaggttacc gtgtcacgtg acgggaaaaa caaccctgtc gtggatgtgt cagaactgat
     5581 ccgtgtatac ggaaatgtga atatccctga gaaacaaatt cccggcattt cacaccgtga
     5641 aaatacacaa aaaaatttcc cacaggaaca gcttctggca atgcaaaagg aactggccga
     5701 tctccggcag gctgtcaccc tgatgctgga agataaaaca tcccgggaag aagaacgccg
     5761 ccagcacgat gatgagcgcc ggaaactcca ggccgaagtg gacagattga caacagaact
     5821 gacacagaag aaaaaacgct tctggtccgg ctggtttagc tgagaactta ccgaagggaa
     5881 tggagaacat gacgaagcag gaaaaaactg ccctcaatat ggctaaattc attaaggatc
     5941 agtcgctgct gttgctggaa aagctgaacg agctggacat ggataccccg gccgacatgt
     6001 gcgaacggct tcacgaggat gcggaaacac tctggcaggt gatgcgggat aagctcgggg
     6061 aagaataaca gcaccggcca tgccggtgct ggcgttattt tttgccgaac ggccaccacc
     6121 ggcgggcggg tttgtcctcc ggcagttcag gcaccggcac gttaaaatag cggctgttat
     6181 attcccgcat cagctccgct ttcagcttct cgttcaccgc gctttccccg tgccgctcgc
     6241 tgaccacctc aatcatcgcg ttaatctgct ccggtgagca cttcccggca tcaaccacct
     6301 gcacaccgtt ctcgatggcg aaccgctcca cctttcgata gaggtaatca cacagcttca
     6361 ttcctgtggg tttgtcccgt tcccattgcg gattttcacc ggtgaaaaag tacgtggctt
     6421 tttcgtcgct gttgagccgc tcgtcataca tcccgatgga tttcatcatc gccagatgct
     6481 gggcccggtc gacaaaacag aaaacatacg ccccggtctg ccaggcgagc accaccatgt
     6541 cttccagatg ctcgctggtt ggcataatgc agcgctcgcc gtctttgtct tccacgtcct
     6601 caataaatac catgtacagc gccataatcc ctcactccgt tgtcataaat ttaaagcacg
     6661 cgaacagcct ctcccggtcc agctccaccg ttttgcgggc ctcgccggtc tgtggattca
     6721 cgatttcatc caccgccaga atcgcccgct gtacccggac aagcgtcggc cggtcagggc
     6781 agatgtacca gatttcatcg atgccgttcg cccggcggtt gaacagatac ccggacacca
     6841 cttcctgata ccgcctgcgg ctcttgacgg tgcgctctac ctcaaatgcc accgttctgc
     6901 cgtccagctc cgccagcgcg tccggcactt tcatcccttt cagggccata cggtgcaggt
     6961 accgccagcc ggtggccccc cggccctcca tggagaggcg cagggactgc acggccagcc
     7021 ggtgcggcag ctgcgccgcc gacacccttg acggctgaaa cggaataacg tccgtcagcg
     7081 gctcgtcctc gtcgaacgag aacgccaccc cgtgcggcgt cagcccccac acgctgacct
     7141 gacggccgtc aaccgggtat ttcgcccgga ccagcagccc tttcgccacc agactgtcca
     7201 ggaagcggta aaccggctgt accgccgcaa tccccagccg tcgcgcaacc agctccgccg
     7261 tggaacacag ctcttcccgc agccagcgca gggtcagccg tgttttctcc gcgttccgcg
     7321 cctgccgctt cgcgtggctg acgttgtact gctccagtgt catcagtaaa gccccgcaaa
     7381 atcatcatcc gcttttggtc cgccgccgtc ggccggaggc aggtactccg ccagcgggtc
     7441 cgttttcgcc agcaccggga aggttttcag cgaaatttcc cggcgctcaa cccgcagcgg
     7501 gctgctgaac gccagccgcg ccacgcccag cccggtcatc accgcgaacc cgtcaggcag
     7561 gtgttgcagg gtgttcacgt caaacagcgg ccgcgcctcc ttctggatga agctgtcgcc
     7621 gctgatgtgc tccacgttcc ccgcctcccg gctggcccgg cggcgctcca cgtccacccg
     7681 gatctccccg gtctggcccg ccgcccactg cgcggactcc tggctggccg cgcgatagaa
     7741 ccagcggatc ggcgtgttat ccagtaccgt ggttttcaca aaatcagcgg gcagatcctg
     7801 cccgcactga ccgaagtcgc ccagcgactg gtgcgcgagc agcaggttgc agtcccggct
     7861 gcgcagcgtc cccagcgcgt tcagcacgta cttcgacagc agatatttaa tctcatcgag
     7921 catgatgctc gcgtgcggcc acctccggaa ctcatcccgc gcaatgatga tttgcgcgca
     7981 gcgggcaaac agcatcttct gcacccggat caccgcctca tcgtccatcg agccgatgac
     8041 gtacaggcag ccgccgccgt tgattatccc cgccacatcg ataccgctgt ccgtctgcag
     8101 gacgctcagg tcggccacgt tctccagctt ggtgatcagc cccttcacgt cctttttcag
     8161 ggcctccggc aacgcgtacg ccgccgccag cacctgctga atgtttgccc cctgcgggaa
     8221 ctgctccgca atcagcttcg ccgctttctg ctcgctgatg cggtagtggt ccgccacgtc
     8281 gccggtctca ctcaggttaa acccggcaac cagcagattc ttcagggcgt actggtcgag
     8341 atcgcggaac agattcagct gcgccggttt ccccggccgc agatccagca gggtgaagtc
     8401 cgggcagtgc gcccggaaga cgtgcggcgc ccacgcgtcc cctttggggt cgaagtacac
     8461 cacagcgtcc ccgaaatcgc gtacgcagcg aatgagcgcg ttggtggcca tcgcgctctt
     8521 gccgctcccg ggcaggccga ggatctgaat attggtcttg cgccaggtat cacgagggac
     8581 cagcaccggc tggccctgct catccttacc cagaaacacc gcctgctgtt tccgggcctg
     8641 ctccgtggaa aaatcggcca gcgcgttaac cggtaccggc tccccgaaat gcaggtggcg
     8701 gacgtctgtc atttctccgg tccgctggct gctgcgccgg ctctcctgat tcagtttcgc
     8761 gttcaccgcc tcgtcagcgg ccgcaaaatg accatcaagc agcaggaacg ccagcagccg
     8821 cgccagaagg acgccggcga caagccctcc ggcacaccag gctatcccga acaggttagc
     8881 cggctgcagc aggtaataat ctgcgttcgc atagaggtcc gggcgcagga cggtttctcc
     8941 ccagctctcg ataaacacgg cgacaaccag cccccccaga agggccagac cggcagcgcc
     9001 ggtcagccag tacgcgtagt tccagacatg tcgccgtgcc ctgaccatcc gcccgggtga
     9061 atccagcgag gccagcaccg aaaatggcac agtccggtta ttatcgcgct tatagcgatc
     9121 ggcatactcc cgccgcgcct tatggctcag ccccacaaaa cgcattcggc cggcccagag
     9181 ttcagccaga ccgaaaaaca ggccgataac cagaaaaaaa ccggccagac tgtaatactc
     9241 ccggtacagc cagctgaccc acgggtaaat ctgccgggcc agctccggga tatgccactg
     9301 ccaggcaagc gaatccgtat taaacatgca atctctctcc ttcagacgac gggtttaccg
     9361 gcgtgatgcg cagcccccgc tgcacaagcc gcggacatgc cccaaggcat ggcggcggcg
     9421 gtcacgtccg tggtgattgc gttcagcttt caccgcggag gccagcctct ttccgcccca
     9481 tgcggaaaag gcgcagcccc ggaggaacaa cgagcacgcc ccagcgtgtg agaggaaaag
     9541 gcggcacccc agtgcggtct tttccggtgt tccggccccc gtcagagttc gccatagcga
     9601 gcggctgacc acccgcaaaa tcaggagcag ctttgctgct tcgtcaggtg cgtccagcac
     9661 cgtggatttt tgtgggtggt caggcgtgag cctgacgggg gccgggtgaa tttctggcgt
     9721 ggccggcgtc cagccggaca gagccagaaa gaggggaaaa gaccgtgcgt cggtggcgtc
     9781 cagccagcgt aagcaaggtc ttttcccctg aagtcagcca cgccccagcg gggatgactt
     9841 gcatttctcc gcaaggagaa aaaagcaaca gagataggtt aacgtaaaat gttcagcgaa
     9901 aaaccgacat ggttcaccta tcctgataat tgatcgtcag ggaatagaaa gacttaatca
     9961 ggggacaata tcccacatca gcgcgtccgg tgaccgtctt gcaggtgccg gggaaattgc
    10021 gcctcctcgc tcactcggtc gctacgctcc gggcgtgaga
//
