LOCUS       NZ_CP047017             3133 bp    DNA     circular CON 19-JUN-2023
DEFINITION  Escherichia coli strain 19-5 plasmid pCol4401, complete sequence.
ACCESSION   NZ_CP047017
VERSION     NZ_CP047017.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13563036
            Assembly: GCF_018279125.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Liu,Z. and Chen,P.
  TITLE     Emergency of CMY-2-harbouring and mcr-1-harboring plasmid in a
            clinical isolate of Cronobacter sakazakii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Liu,Z. and Chen,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2019) College of Veterinary Medicine, Northwest
            A&F University, No. 3 Taicheng Road, Yangling, Shanxi 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler Version v. v0.48
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 80.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/19/2023 03:19:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,204
            CDSs (total)                      :: 5,075
            Genes (coding)                    :: 4,808
            CDSs (with protein)               :: 4,808
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 267
            CDSs (without protein)            :: 267
            Pseudo Genes (ambiguous residues) :: 0 of 267
            Pseudo Genes (frameshifted)       :: 77 of 267
            Pseudo Genes (incomplete)         :: 171 of 267
            Pseudo Genes (internal stop)      :: 56 of 267
            Pseudo Genes (multiple problems)  :: 36 of 267
            Pseudo Genes (short protein)      :: 3 of 267
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP047017.1.
            Bacteria and source DNA available from xiaoping song , Traditional
            Chinese Veterinary Medicine Lab,College of vetrinary Medicine,
            Northwest A&F University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="19-5"
                     /isolation_source="heart"
                     /host="swine"
                     /db_xref="taxon:562"
                     /plasmid="pCol4401"
                     /geo_loc_name="China:Yangling"
                     /collection_date="2019-05-14"
     gene            complement(join(2425..3133,1..44))
                     /locus_tag="GQF95_RS25715"
                     /old_locus_tag="GQF95_25650"
     CDS             complement(join(2425..3133,1..44))
                     /locus_tag="GQF95_RS25715"
                     /old_locus_tag="GQF95_25650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016249639.1"
                     /translation="MDTTSLISSISVPRLSFYETFLGCATDQEKVGAYVAYQDLSGDFF
                     CLVQMIEVALRNAINNTIKANHGPAWYDSIPQTKKSQDLVLNAKQKALDECGVRYTDDD
                     VICRLTFGFWVYMLDAPYRDTQRPCYIWTPENKAKTFPHAKNPLGGGDMKVKALFDEFH
                     KVLLFRNRLFHHEPIWKKHHCKSHSQAINNMLKEFNFLMNALSIVSNEKKELIEFIGHD
                     RRFYERCTIEYVMGIIGRIKCRELKVAG"
                     /name="hypothetical protein"
     gene            complement(503..826)
                     /gene="mobA"
                     /locus_tag="GQF95_RS25720"
                     /old_locus_tag="GQF95_25655"
     CDS             complement(503..826)
                     /gene="mobA"
                     /locus_tag="GQF95_RS25720"
                     /old_locus_tag="GQF95_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            1111..1305
                     /locus_tag="GQF95_RS25725"
                     /old_locus_tag="GQF95_25660"
     CDS             1111..1305
                     /locus_tag="GQF95_RS25725"
                     /old_locus_tag="GQF95_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_025492124.1"
                     /translation="MNKQQQTALNMAKFIKSQSLTLLEKLDSLDADAQAAMCEKLHELA
                     EELQNSIQTRFESESGTGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2257..2400
                     /locus_tag="GQF95_RS25730"
                     /old_locus_tag="GQF95_25665"
     CDS             2257..2400
                     /locus_tag="GQF95_RS25730"
                     /old_locus_tag="GQF95_25665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152918943.1"
                     /translation="MNTKVLFVKKALKSITCAILSLLPALSRAVTELRSLSAARLKAGK
                     SF"
                     /name="hypothetical protein"
     Typing          complement(836..1073)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="99.0"
                     /evalue="2.15e-118"
     Typing          1604..2053
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.494"
                     /coverage="90.0"
                     /evalue="1.02e-154"
     Typing          complement(507..826)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.32e-62"
ORIGIN
        1 aatcttggta cagaaattga gctaattaaa cttgttgtat ccatgaaaac tcacttatct
       61 ctttactatg aatatttttc agactaccat tacaacattc gaggagcagg cgaactaccg
      121 tttttctgtg tacctctaga gcgtaaaata taacaaaaaa gcccactaca gagtgggctt
      181 ttttgttata tttatctata tgaaacaacc agttatagtt attgtttcat aataagaatt
      241 gaatactctt cacaatccac aacatcagaa aatactttgc tgccaattta ccataactcc
      301 ccttacgcgc accgctaaaa cgcgctgaac gcgattctgc cgcaaacacc gcaaaaagag
      361 atgttttacc cagtgaaaac cggaaaaatg cgtcaggacc gttttcagct cactgcatag
      421 ctatgcatga aagtgaatgg cgatcggttt ggggccttac ggcgttcata ccgtctgttt
      481 tcgacagttt ctctccggga agctaatctg ccataagcct ggataacagg gcccggtgat
      541 actccgtaat agcgatcagc acctccgcat actctctgtc cccgacacgc ttgccgtcga
      601 taaagagttt tttctgtaac gcccccagtc tcataacttc tttcagtacc cgatcatcag
      661 tcagtgagtt aactttctta ccgagagctg ccgcccgtaa aaaaccggaa acggtcttgc
      721 cgcattcagc agcccttttt ctgatttcct ggtcttcttc tgccgtcaga cgaacagggc
      781 gactgatagc ccgcctgcgc gtattacttc cacttcgttt tgtcataaaa catggctccg
      841 tatctgacat ggggtgtcgg ggcgaagccc tgaccagggt aattgtaata gcgcgcatgt
      901 atgcgcggta taacaattac acatcctgtc ctgtcagcaa gttcgaatta ccgacaaagg
      961 cacggcagtt ttttggcagt gacagtattg tcaggcagaa atgcccgtca aagagtcaga
     1021 cggcagaata ccggcggcaa aactgtcgaa aagtggtacg cgatataatc acacgacgca
     1081 gatgacagac agaacggaga aaacagaaag atgaacaagc agcagcaaac cgcacttaac
     1141 atggcaaaat tcataaaaag ccagagcctg acgctgctcg aaaaactgga ctcactcgat
     1201 gctgatgcac aggccgccat gtgtgagaaa ctgcacgaac tcgcagaaga actccagaac
     1261 agcatacaga cacgctttga atcggaaagt ggcaccgggg aataacagtg ttcctacacc
     1321 agacaggatg cagaacgata taccggtgcg taaggagaaa ataccacata aggcgctctt
     1381 ccgcttcctc gctcactaac tcgctacgct cggtcgttcg gctgcggcga gcggtatcag
     1441 ctcactcaaa gacggtaatg aggttatcca cagaatcagg ggataacgcc aaaagaaaca
     1501 tgtgagcaaa aaacaagaac ccgaaaaagg ccgcgccggt ggcgtttttc cataggctcc
     1561 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1621 gactataaag ataccaggcg tttccccctg atggctccct cgtgcgctct cctgttcctg
     1681 cctttcggtt taccggtgtc attccgctgt tatggccgcg ttatctcatt ccacgcctga
     1741 cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
     1801 agtccgacta ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaaggcacg
     1861 acttatcgcc attggcagca gccactggta actgatttag aggagttagt cttgaagtta
     1921 cgtgccagtt aaggctaaac tggaagaaca agttttggtg actgcgctcc tcttaagcca
     1981 gttaccgagg ttcagaaact tgccggttgc tgaaccttcg aaaaaccacc ttgccgggtg
     2041 gttttttcgt ttttaagatt acgatgggcg cgatatgaac tcaaggaaat ccgcaattaa
     2101 attataaaag tttgtggatt aatggaaaaa ctgatgtgaa tcagctattt atccttaaga
     2161 tttgatgtca gatatggtat aattaaccat tcaaatagat ggaataaaaa gtcaacgctg
     2221 ccattagaac ggcataagaa atagaggatt atgaaaatga atactaaagt tctttttgtg
     2281 aaaaaagcct tgaaatcaat tacttgcgca attctttcgc tcttaccggc tttaagtcgc
     2341 gcagtaacag agttacgtag cctttcggct gcgcgactta aagccggtaa gagcttttga
     2401 ttgtatcaca tttttcctta aaagtcaacc ggctacctta agctccctgc acttaatcct
     2461 ccctataatc cccataacat actctatcgt gcagcgttca taaaagcgcc tatcgtgccc
     2521 aataaactca ataagttcct ttttctcgtt agagacgata ctcaaggcat tcattaaaaa
     2581 attgaactct ttcaacatat tatttatggc ttgactatgc gacttgcagt ggtgtttttt
     2641 ccaaataggc tcatggtgaa aaagtcgatt cctaaacaaa agaactttat gaaactcgtc
     2701 aaacaacgct ttaaccttca tatccccccc acccaaagga ttcttcgcat gaggaaatgt
     2761 ttttgccttg ttctctggag tccagatgta gcacggcctt tgcgtatccc tatatggggc
     2821 atctaacata taaacccaaa agccaaaggt tagacggcaa ataacatcat catccgtata
     2881 ccttacgcca cactcatcta gcgctttttg tttcgcattt aaaacaagat cctgagattt
     2941 ctttgtttga ggtatagagt cgtaccaagc aggcccatga ttagctttga ttgtattgtt
     3001 tatcgcatta cgcagagcaa cctcaatcat ctggacaaga caaaaaaaat cgccagacag
     3061 atcttggtag gcaacgtatg ccccaacctt ttcttgatca gtagcgcacc caagaaatgt
     3121 ttcatagaaa gat
//
