LOCUS       NZ_CP046965            10060 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WCHKP020130 plasmid
            p2_020130, complete sequence.
ACCESSION   NZ_CP046965
VERSION     NZ_CP046965.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN07609131
            Assembly: GCF_002852675.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Liu,L., Liu,Y., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP020130
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Liu,L., Liu,Y., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 10060)
  AUTHORS   Liu,L., Lu,Y., Lin,J., Yang,B., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-SEP-2017) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:19:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,685
            CDSs (total)                      :: 5,564
            Genes (coding)                    :: 5,444
            CDSs (with protein)               :: 5,444
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 45 of 120
            Pseudo Genes (incomplete)         :: 77 of 120
            Pseudo Genes (internal stop)      :: 14 of 120
            Pseudo Genes (multiple problems)  :: 16 of 120
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046965.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP020130"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="p2_020130"
                     /geo_loc_name="China: Sichuan, Chengdu"
                     /collection_date="2017-02-20"
     gene            join(9982..10060,1..101)
                     /locus_tag="CLQ86_RS28240"
                     /old_locus_tag="CLQ86_27740"
     CDS             join(9982..10060,1..101)
                     /locus_tag="CLQ86_RS28240"
                     /old_locus_tag="CLQ86_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(128..658)
                     /locus_tag="CLQ86_RS28245"
                     /old_locus_tag="CLQ86_27745"
     CDS             complement(128..658)
                     /locus_tag="CLQ86_RS28245"
                     /old_locus_tag="CLQ86_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(665..1396)
                     /gene="mobC"
                     /locus_tag="CLQ86_RS28250"
                     /old_locus_tag="CLQ86_27750"
     CDS             complement(665..1396)
                     /gene="mobC"
                     /locus_tag="CLQ86_RS28250"
                     /old_locus_tag="CLQ86_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(1396..3360)
                     /locus_tag="CLQ86_RS28255"
                     /old_locus_tag="CLQ86_27755"
     CDS             complement(1396..3360)
                     /locus_tag="CLQ86_RS28255"
                     /old_locus_tag="CLQ86_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4844..4993)
                     /locus_tag="CLQ86_RS28260"
                     /old_locus_tag="CLQ86_27760"
     CDS             complement(4844..4993)
                     /locus_tag="CLQ86_RS28260"
                     /old_locus_tag="CLQ86_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5078..5335)
                     /locus_tag="CLQ86_RS28265"
                     /old_locus_tag="CLQ86_27765"
     CDS             complement(5078..5335)
                     /locus_tag="CLQ86_RS28265"
                     /old_locus_tag="CLQ86_27765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(5345..7030)
                     /locus_tag="CLQ86_RS28270"
                     /old_locus_tag="CLQ86_27770"
     CDS             complement(5345..7030)
                     /locus_tag="CLQ86_RS28270"
                     /old_locus_tag="CLQ86_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7357..7602
                     /locus_tag="CLQ86_RS28275"
                     /old_locus_tag="CLQ86_27775"
     CDS             7357..7602
                     /locus_tag="CLQ86_RS28275"
                     /old_locus_tag="CLQ86_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7876..8247
                     /gene="tolA"
                     /locus_tag="CLQ86_RS28280"
                     /old_locus_tag="CLQ86_27780"
     CDS             7876..8247
                     /gene="tolA"
                     /locus_tag="CLQ86_RS28280"
                     /old_locus_tag="CLQ86_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8244..8609
                     /locus_tag="CLQ86_RS28285"
     CDS             8244..8609
                     /locus_tag="CLQ86_RS28285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            8784..9062
                     /locus_tag="CLQ86_RS28290"
                     /old_locus_tag="CLQ86_27790"
     CDS             8784..9062
                     /locus_tag="CLQ86_RS28290"
                     /old_locus_tag="CLQ86_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9050..9364
                     /locus_tag="CLQ86_RS28295"
                     /old_locus_tag="CLQ86_27795"
     CDS             9050..9364
                     /locus_tag="CLQ86_RS28295"
                     /old_locus_tag="CLQ86_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            9528..9956
                     /locus_tag="CLQ86_RS28300"
                     /old_locus_tag="CLQ86_27800"
     CDS             9528..9956
                     /locus_tag="CLQ86_RS28300"
                     /old_locus_tag="CLQ86_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     BGC             345..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4163..4741
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(669..1396)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 acgagctgga catggatacc ccggccgaca tgtgcgaacg gcttcacgag gatgcggaaa
       61 cactctggca ggtgatgcgg gataagctcg gggaagaata acagcaccgg ccatgccggt
      121 gctggcgtta ttttttgccg aacggccacc accggcgggc gggtttgtcc tccggcagtt
      181 caggcaccgg cacgttaaaa tagcggctgt tatattcccg catcagctcc gctttcagct
      241 tctcgttcac cgcgctttcc ccgtgccgct cgctgaccac ctcaatcatc gcgttaatct
      301 gctccggtga gcacttcccg gcatcaacca cctgcacacc gttctcgatg gcgaaccgct
      361 ccacctttcg atagaggtaa tcacacagct tcattcctgt gggtttgtcc cgttcccatt
      421 gcggattttc accggtgaaa aagtacgtgg ctttttcgtc gctgttgagc cgctcgtcat
      481 acatcccgat ggatttcatc atcgccagat gctgggcccg gtcgacaaaa cagaaaacat
      541 acgccccggt ctgccaggcg agcaccacca tgtcttccag atgctcgctg gttggcataa
      601 tgcagcgctc gccgtctttg tcttccacgt cctcaataaa taccatgtac agcgccataa
      661 tccctcactc cgttgtcata aatttaaagc acgcgaacag cctctcccgg tccagctcca
      721 ccgttttgcg ggcctcgccg gtctgtggat tcacgatttc atccaccgcc agaatcgccc
      781 gctgtacccg gacaagcgtc ggccggtcag ggcagatgta ccagatttca tcgatgccgt
      841 tcgcccggcg gttgaacaga tacccggaca ccacttcctg ataccgcctg cggctcttga
      901 cggtgcgctc tacctcaaat gccaccgttc tgccgtccag ctccgccagc gcgtccggca
      961 ctttcatccc tttcagggcc atacggtgca ggtaccgcca gccggtggcc ccccggccct
     1021 ccatggagag gcgcagggac tgcacggcca gccggtgcgg cagctgcgcc gccgacaccc
     1081 ttgacggctg aaacggaata acgtccgtca gcggctcgtc ctcgtcgaac gagaacgcca
     1141 ccccgtgcgg cgtcagcccc cacacgctga cctgacggcc gtcaaccggg tatttcgccc
     1201 ggaccagcag ccctttcgcc accagactgt ccaggaagcg gtaaaccggc tgtaccgccg
     1261 caatccccag ccgtcgcgca accagctccg ccgtggaaca cagctcttcc cgcagccagc
     1321 gcagggtcag ccgtgttttc tccgcgttcc gcgcctgccg cttcgcgtgg ctgacgttgt
     1381 actgctccag tgtcatcagt aaagccccgc aaaatcatca tccgcttttg gtccgccgcc
     1441 gtcggccgga ggcaggtact ccgccagcgg gtccgttttc gccagcaccg ggaaggtttt
     1501 cagcgaaatt tcccggcgct caacccgcag cgggctgctg aacgccagcc gcgccacgcc
     1561 cagcccggtc atcaccgcga acccgtcagg caggtgttgc agggtgttca cgtcaaacag
     1621 cggccgcgcc tccttctgga tgaagctgtc gccgctgatg tgctccacgt tccccgcctc
     1681 ccggctggcc cggcggcgct ccacgtccac ccggatctcc ccggtctggc ccgccgccca
     1741 ctgcgcggac tcctggctgg ccgcgcgata gaaccagcgg atcggcgtgt tatccagtac
     1801 cgtggttttc acaaaatcag cgggcagatc ctgcccgcac tgaccgaagt cgcccagcga
     1861 ctggtgcgcg agcagcaggt tgcagtcccg gctgcgcagc gtccccagcg cgttcagcac
     1921 gtacttcgac agcagatatt taatctcatc gagcatgatg ctcgcgtgcg gccacctccg
     1981 gaactcatcc cgcgcaatga tgatttgcgc gcagcgggca aacagcatct tctgcacccg
     2041 gatcaccgcc tcatcgtcca tcgagccgat gacgtacagg cagccgccgc cgttgattat
     2101 ccccgccaca tcgataccgc tgtccgtctg caggacgctc aggtcggcca cgttctccag
     2161 cttggtgatc agccccttca cgtccttttt cagggcctcc ggcaacgcgt acgccgccgc
     2221 cagcacctgc tgaatgtttg ccccctgcgg gaactgctcc gcaatcagct tcgccgcttt
     2281 ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc tcactcaggt taaacccggc
     2341 aaccagcaga ttcttcaggg cgtactggtc gagatcgcgg aacagattca gctgcgccgg
     2401 tttccccggc cgcagatcca gcagggtgaa gtccgggcag tgcgcccgga agacgtgcgg
     2461 cgcccacgcg tcccctttgg ggtcgaagta caccacagcg tccccgaaat cgcgtacgca
     2521 gcgaatgagc gcgttggtgg ccatcgcgct cttgccgctc ccgggcaggc cgaggatctg
     2581 aatattggtc ttgcgccagg tatcacgagg gaccagcacc ggctggccct gctcatcctt
     2641 acccagaaac accgcctgct gtttccgggc ctgctccgtg gaaaaatcgg ccagcgcgtt
     2701 aaccggtacc ggctccccga aatgcaggtg gcggacgtct gtcatttctc cggtccgctg
     2761 gctgctgcgc cggctctcct gattcagttt cgcgttcacc gcctcgtcag cggccgcaaa
     2821 atgaccatca agcagcagga acgccagcag ccgcgccaga aggacgccgg cgacaagccc
     2881 tccggcacac caggctatcc cgaacaggtt agccggctgc agcaggtaat aatctgcgtt
     2941 cgcatagagg tccgggcgca ggacggtttc tccccagctc tcgataaaca cggcgacaac
     3001 cagccccccc agaagggcca gaccggcagc gccggtcagc cagtacgcgt agttccagac
     3061 atgtcgccgt gccctgacca tccgcccggg tgaatccagc gaggccagca ccgaaaatgg
     3121 cacagtccgg ttattatcgc gcttatagcg atcggcatac tcccgccgcg ccttatggct
     3181 cagccccaca aaacgcattc ggccggccca gagttcagcc agaccgaaaa acaggccgat
     3241 aaccagaaaa aaaccggcca gactgtaata ctcccggtac agccagctga cccacgggta
     3301 aatctgccgg gccagctccg ggatatgcca ctgccaggca agcgaatccg tattaaacat
     3361 gcaatctctc tccttcagac gacgggttta ccggcgtgat gcgcagcccc cgctgcacaa
     3421 gccgcggaca tgccccaagg catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc
     3481 tttcaccgcg gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa
     3541 caacgagcac gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg tcttttccgg
     3601 tgttccggcc cccgtcagag ttcgccatag cgagcggctg accacccgca aaatcaggag
     3661 cagctttgct gcttcgtcag gtgcgtccag caccgtggat ttttgtgggt ggtcaggcgt
     3721 gagcctgacg ggggccgggt gaatttctgg cgtggccggc gtccagccgg acagagccag
     3781 aaagagggga aaagaccgtg cgtcggtggc gtccagccag cgtaagcaag gtcttttccc
     3841 ctgaagtcag ccacgcccca gcggggatga cttgcatttc tccgcaagga gaaaaaagca
     3901 acagagatag gttaacgtaa aatgttcagc gaaaaaccga catggttcac ctatcctgat
     3961 aattgatcgt cagggaatag aaagacttaa tcaggggaca atatcccaca tcagcgcgtc
     4021 cggtgaccgt cttgcaggtg ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc
     4081 tccgggcgtg agactgcggc gggcgctgcg gacacataca aagttaccca cagattccgt
     4141 ggataagcag gggactaaca tgtgaggcaa aacggcaggg ccgcgccggt ggcgtttttc
     4201 cataggctcc gccctcctgc cagagttcac ataaacagac gctttttcgg tgcatctgtg
     4261 ggagccgtga ggctcaacca tgaatctgac agtacgggcg aaacccgaca ggacttaaag
     4321 atccccaccg tttccggtag gtcgctccct cttgcgctct cctgttccga ccctgccgtt
     4381 taccggatac ctgtccgccg ttctcccttg cgggaagtgt ggcgctttct catagctcac
     4441 acactggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta agcaagaact
     4501 ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt ccaacccggg
     4561 aaagcacgca aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg
     4621 tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga
     4681 cagatttggt tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt tccccaactg
     4741 acttaacctt cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt
     4801 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctactga accgcgatcc
     4861 ccgtcagttc agaggaggac gatggtgcca ccgttccgcc ctgaacatcc cggatataat
     4921 ttgcctgaca tgcggccaga agaaagccag caacaatcaa tataaaaaga aaaatagtct
     4981 ttaccttttt catatacttt ccctgtattt aaagactccg gcattcatcc ctgaacaccg
     5041 gaagtcagta tttcaaatca tccttgataa ttttttgtta ccagttaccg tcccgataat
     5101 caaaagctat ataacaatta tccacatcag gttttatttg atttttaaaa aatggctgta
     5161 gtaaaggaac ccattctttc ttcaaatcaa actcaccatt attaatatta tcctttaaag
     5221 gcattcccaa gctttcaatg acagaaccat cgtcaccaaa gtctttagaa tactcttccc
     5281 ctttaaactc ttcagttttc ttatcaaacc atgtcaaacg taatttaagc cccatattta
     5341 cctctcaaag atatttttta atgttccgtt ttggatctgg ccctttaacc tgcttacccg
     5401 ttttgggatc aaatgcgcct aggtgttcgc catcactggc acgataccct tcaagctcac
     5461 cgtgctggga atcccattca taaattttac gttttttatc gccataccat cgggcccgct
     5521 taccaccacc accttgttta ggtgttttag gatcacctcg ctttaactct ccaagtccct
     5581 ttatgtcttc tgttttaggt actggatgat aatcatggcc gtagtcttta gtccccttgc
     5641 gaggcttgtt cttttcctca tttaacttat tttctgcatc cttcgctttt ttctcctttt
     5701 gtttccggct ctccatcgcc ccggtaagtg cagccttatc tctggataag gattcattca
     5761 ttgcgtttaa agatgcctga gccgctttta atttctcatt ctgattctga atatccttat
     5821 caagatagcg aacctgatcc tgccacccgg agccatattc atgaggcggg ttataagtca
     5881 caaaatcatc tttatttgcc tctgcctctt ttaccttttt atcggcttcc tgaacagcag
     5941 cccttgcacc ggggagcgcc ttctctgtcg atgcaatgcg gctgtttaag gtcgcaatat
     6001 ttttatcttc ggcatccagc tcagctttcg ctttatcata gtctcttttc agcccctctt
     6061 ccggatgagc ggcgtcccat gcctgctggc gacgcttttc ttcctcctga cgctgtttta
     6121 cctgtgccgg ggtaagaaca tctgtcaccg acacataaac cggcttctgt ccggtctctt
     6181 tcgggaaacg aataacggcc tcacgggagt taccaccggc tgtaaaacca gccggacgtg
     6241 aatcaccttt ttcagcaaca atgccttttg gcggggcttt cgctgtcgga acacctttcg
     6301 gtacgctcac ctgcagagac gggagacccg gaatcgacac actgaatacc cccggacgtt
     6361 ttgtcggttt cgcatccacc acagggacac tcattggccg acctgcgaca accgcaatat
     6421 gctgccgctc atccttcacc acatccacaa cccgttgtct gacacgaacc gtcgcctggt
     6481 ccagtggtaa agtacttgcc ggagtctccg tcaccgcatc ggccggtaat gacgtcataa
     6541 tttttgacat cattttcgga tcatcttttg caatctcaga aggcagcaca ccgtagatcg
     6601 caatccccca cagaccaaac ttaaacggcc ccttcagggc agccagcaca tcagcaacgg
     6661 cggccgacaa cgcatcgccg gaaactgata aagccagtcc ttcagccccc ggagtagcca
     6721 gggcaggcag accgaaggcc atcgcagtgg ccgaagactg tccgccacca gagctaccgc
     6781 tatgattgcc gctgttacca ccattaccac cgggtttact cccgccgtta ccaaactccc
     6841 ccggattata gttatgaata tctccattcg gcgtatgcgt cgtaccccag cccggcccgc
     6901 tgtttggatc cgtaccacca cctgatgaag ggccaccttt accagacgta ccgttcacat
     6961 tcccactggc ctgaccacca ttatgtccca gacctgaatt acccggacct cgaccgtctc
     7021 caccactcat accgttttcc tctttgacat ttaaaacaat aaattaaagc caacatactg
     7081 tacatataca cagtataatt attaccttac tcctcccgtt ttcaggtgtc aatatatgag
     7141 attagttctc atcttcaggc acaaaaaagc gcccggagcc acctgtaagc agatcgcgtc
     7201 ttttcccgaa aatttctcat ggcaacaaat tttattattt aagacattca tcacacttac
     7261 atttcagatg gtatagacac atctgaaagc taactggcat gaggagagga cctgcggtta
     7321 aactgttcgg gaaacctgag acgcccggag gccactatga cgcacctgaa actgactccc
     7381 gctgaagaaa agcgcctggc ggacgccttt gtcgctcagg cactggccat gggcctgaaa
     7441 gggaacaaaa cgctgccaaa ggcggtaaaa caacaggtgc gggaaagggt gcttcgtgcc
     7501 attcaggacg aacgtgaagc caggcgggcg caggaaatgc gtcgggagaa atacgaggct
     7561 acggaaacca ccttcacctg gcagccacag cgccgccggt aaggggtatt taccataacg
     7621 tcgcattgcc cgcaccgctc agacggtctg agggcgtttc cggcggctac gggataaaac
     7681 aggcaacaaa ccacccggaa aaccgccgga aacgcgcccg gtaaattgta acccgctgca
     7741 tgcctatgca cgcaactgca tggcgggccg tttggtgtaa aaacgatacg ataagccgtg
     7801 tttatgtaaa tgaacgtggc cgtaaagagt gacaaatata catgtaacat tttttactga
     7861 caggtcatca acccgatgcg tttaataccc tttatcctgc tggcattgtg cagcccctta
     7921 ctcctgacgg cctgtaagca ttccaccacc cagccgccga aaatttatac tctggataag
     7981 tcatctaaaa tcagtagcta cgctgcggcc atcaagaccg ctgttgaaat gcagatgttt
     8041 gaaccagagg cctatgcggg aaaaatgtgc accgtgcgga tatccataca acgtgatggc
     8101 actctgaaca gcgccattgc tgaaggagga gatccgaaac tctgtaaagc agcaatatca
     8161 gccgttaccc acgccaaaat tccgccggca ccggatgaaa atacctggca aatattcaaa
     8221 aacgcccctc tggatttcag accatgaaag cttttactct gacagtgctg gcggccatgt
     8281 tcctcactcc ggtacatgcg ataccactaa aacccgtaac cacactgcag ccaacctacc
     8341 cggtacaggc cgcagcaaaa cgcatcgaag gtcaggtgac cgccacgttt gatattctgg
     8401 acgatggacg agtggacaac gtgtcgctta ccggctgtca cctttttttt cgggaaacgc
     8461 tgacggcaat gaggcactgg ctatatcccg cagggcaccc ggcaatgcga caggttataa
     8521 attttgaatt cagtctgaaa aatgacggct tacaggatcc gcgaccagtc accacccaaa
     8581 agctcaatga tgatggttgc cgccagtaac aggtccttta acccgcaggc tgcaatagtc
     8641 ctggatgatg agtgagcctg tagcagccct gattagtgga agcgaagctg aaaaatgggc
     8701 agcaaaagcc gtatttctgg caaatagcgc cgccacggat taaagtagtc atgtgtaata
     8761 catatgacta caaaggggct tttatgtcat cagcaacatc catcaggctc gatgaagaac
     8821 ttaaggaccg gctgaaaacg ctggccgatg accggcaccg ctcagcgcac gcgctaatgc
     8881 tggaggcaat caccgaatac atcgaccggg aagagaagcg cagccagtac ctgcgggacg
     8941 gacaggccgc atggcagcac tatcaggaaa cgggtctgca ccttactgcg gaagaagcgg
     9001 aagcctggat aagtacctgg ggaactgaga acgagcagga tgctccgcca tgccacaggt
     9061 aacgatttcc gcgctggcac agcgcgattt acaacgcctt caggactttc tcaaaaccaa
     9121 aaatcggctg gcagccagaa aaggaggtga ggtgatcgtc cgggctatcc agcaactgaa
     9181 aacattgcca gacattggcc gcccggtgcc gtttctgccg ctggaatata aggaactggt
     9241 gatcgggttt ggcgacagtg gctatgtgat gctctaccgc cacgaccggg aaatggacca
     9301 gattgtgatc gtcaccgtca ggcatcagaa agaatccggg tatccggggg cagacagcct
     9361 ctaataaccg atgacggggg cgcaattcat tgcgtggccg tcgccggttt gacaagcgca
     9421 gcgcgtcaga aaaccacatc ctgaacggaa aaacgggtaa aattcagcga tacatttacc
     9481 gtgagaaatt acacaccttt cacacccgat aaaatcaggg gtatacagtg gcgaaaacat
     9541 tgctcaacct ttcacaggca gcacaagccg cgggcattac cagaagaacg ctatacaatc
     9601 atgtaaaaca gggaaaggtt accgtgtcac gtgacgggaa aaacaaccct gtcgtggatg
     9661 tgtcagaact gatccgtgta tacggaaatg tgaatatccc tgagaaacaa attcccggca
     9721 tttcacaccg tgaaaataca caaaaaaatt tcccacagga acagcttctg gcaatgcaaa
     9781 aggaactggc cgatctccgg caggctgtca ccctgatgct ggaagataaa acatcccggg
     9841 aagaagaacg ccgccagcac gatgatgagc gccggaaact ccaggccgaa gtggacagat
     9901 tgacaacaga actgacacag aagaaaaaac gcttctggtc cggctggttt agctgagaac
     9961 ttaccgaagg gaatggagaa catgacgaag caggaaaaaa ctgccctcaa tatggctaaa
    10021 ttcattaagg atcagtcgct gctgttgctg gaaaagctga
//
