LOCUS       NZ_CP046960            12746 bp    DNA     circular CON 11-MAR-2024
DEFINITION  Klebsiella pneumoniae strain WCHKP015247 plasmid p2_015247, complete
            sequence.
ACCESSION   NZ_CP046960
VERSION     NZ_CP046960.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN08624641
            Assembly: GCF_003038035.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12746)
  AUTHORS   Lin,J., Liu,Y., Liu,L., Feng,Y. and Zong,Z.
  TITLE     The complete genome of Klebsiella pneumoniae strain WCHKP015247
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12746)
  AUTHORS   Lin,J., Liu,Y., Liu,L., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2019) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
REFERENCE   3  (bases 1 to 12746)
  AUTHORS   Liu,L., Lin,J., Tang,G., Yang,B., Feng,Y. and Zong,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-FEB-2018) Center of Infectious Diseases, West China
            Hospital, Sichuan University, Guoxuexiang 37, Chengdu, Sichuan
            610041, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 03/11/2024 05:18:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,628
            CDSs (total)                      :: 5,507
            Genes (coding)                    :: 5,367
            CDSs (with protein)               :: 5,367
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 45 of 140
            Pseudo Genes (incomplete)         :: 93 of 140
            Pseudo Genes (internal stop)      :: 14 of 140
            Pseudo Genes (multiple problems)  :: 12 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046960.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12746
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WCHKP015247"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2_015247"
                     /geo_loc_name="China: Chengdu, Sichuan"
                     /collection_date="2017-05-09"
     gene            complement(join(<11828..12746,1..230))
                     /locus_tag="C6M24_RS27955"
                     /old_locus_tag="C6M24_27475"
                     /pseudo=""
     CDS             complement(join(<11828..12746,1..230))
                     /locus_tag="C6M24_RS27955"
                     /old_locus_tag="C6M24_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(335..1822)
                     /gene="ltrA"
                     /locus_tag="C6M24_RS27960"
                     /old_locus_tag="C6M24_27480"
     CDS             complement(335..1822)
                     /gene="ltrA"
                     /locus_tag="C6M24_RS27960"
                     /old_locus_tag="C6M24_27480"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            2467..2712
                     /locus_tag="C6M24_RS27965"
                     /old_locus_tag="C6M24_27485"
     CDS             2467..2712
                     /locus_tag="C6M24_RS27965"
                     /old_locus_tag="C6M24_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            2986..3357
                     /gene="tolA"
                     /locus_tag="C6M24_RS27970"
                     /old_locus_tag="C6M24_27490"
     CDS             2986..3357
                     /gene="tolA"
                     /locus_tag="C6M24_RS27970"
                     /old_locus_tag="C6M24_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            3354..3719
                     /locus_tag="C6M24_RS27975"
     CDS             3354..3719
                     /locus_tag="C6M24_RS27975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            3894..4172
                     /locus_tag="C6M24_RS27980"
                     /old_locus_tag="C6M24_27500"
     CDS             3894..4172
                     /locus_tag="C6M24_RS27980"
                     /old_locus_tag="C6M24_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            4160..4474
                     /locus_tag="C6M24_RS27985"
                     /old_locus_tag="C6M24_27505"
     CDS             4160..4474
                     /locus_tag="C6M24_RS27985"
                     /old_locus_tag="C6M24_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            4638..5066
                     /locus_tag="C6M24_RS27990"
                     /old_locus_tag="C6M24_27510"
     CDS             4638..5066
                     /locus_tag="C6M24_RS27990"
                     /old_locus_tag="C6M24_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            5092..5271
                     /locus_tag="C6M24_RS27995"
                     /old_locus_tag="C6M24_27515"
     CDS             5092..5271
                     /locus_tag="C6M24_RS27995"
                     /old_locus_tag="C6M24_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5298..5828)
                     /locus_tag="C6M24_RS28000"
                     /old_locus_tag="C6M24_27520"
     CDS             complement(5298..5828)
                     /locus_tag="C6M24_RS28000"
                     /old_locus_tag="C6M24_27520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5835..6566)
                     /gene="mobC"
                     /locus_tag="C6M24_RS28005"
                     /old_locus_tag="C6M24_27525"
     CDS             complement(5835..6566)
                     /gene="mobC"
                     /locus_tag="C6M24_RS28005"
                     /old_locus_tag="C6M24_27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(6566..8530)
                     /locus_tag="C6M24_RS28010"
                     /old_locus_tag="C6M24_27530"
     CDS             complement(6566..8530)
                     /locus_tag="C6M24_RS28010"
                     /old_locus_tag="C6M24_27530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(10014..10163)
                     /locus_tag="C6M24_RS28015"
                     /old_locus_tag="C6M24_27535"
     CDS             complement(10014..10163)
                     /locus_tag="C6M24_RS28015"
                     /old_locus_tag="C6M24_27535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(10248..10505)
                     /locus_tag="C6M24_RS28020"
                     /old_locus_tag="C6M24_27540"
     CDS             complement(10248..10505)
                     /locus_tag="C6M24_RS28020"
                     /old_locus_tag="C6M24_27540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(10515..>11057)
                     /locus_tag="C6M24_RS28025"
                     /old_locus_tag="C6M24_27545"
                     /pseudo=""
     CDS             complement(10515..>11057)
                     /locus_tag="C6M24_RS28025"
                     /old_locus_tag="C6M24_27545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            complement(11074..11771)
                     /locus_tag="C6M24_RS28030"
                     /old_locus_tag="C6M24_27550"
     CDS             complement(join(11074..11522,11522..11771))
                     /locus_tag="C6M24_RS28030"
                     /old_locus_tag="C6M24_27550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223174630.1"
                     /translation="MASVSVCCPSCSATEGVMRNGKSTAGHQRYLCSHCRKTWQLTFTY
                     AASQPGTHQKIIDMAMNGVGCRATARLMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRAVVAHVFGERTMATLERLLSLLSVFDVVVW
                     MTDGWPMYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     BGC             6822..12746
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          9333..9911
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5839..6566)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 ccattaccac cgggtttact cccgccgtta ccaaactccc ccggattata gttatgaata
       61 tctccattcg gcgtatgcgt cgtaccccag cccggcccgc tgtttggatc cgtaccacca
      121 cctgatgaag ggccaccttt accagacgta ccgttcacat tcccactggc ctgaccacca
      181 ttatgtccca gacctgaatt acccggacct cgaccgtctc caccactcat accgttttcc
      241 tctttgacat ttaaaacaat aaattaaagc caacatgtcg cggtagggat acccgttacc
      301 ggataccccc cgcacagatc ccggcgtgcg cgatttacgc accgggctcc tgcctcgggt
      361 gtctggcggt gaaccgctcc acaggccatg gatgaagaac ccgaaccctt ggtagccatg
      421 cggctgccag tttgtttgct ttcgtccagg tcgtatcatc cttctggctc ctgcgcctga
      481 gcgcccggcg ccagaggttt gttacgtgtg tcctgaactt ctgcatggtg gggaagttgc
      541 ccggtaccga gtgatagttc aggtatccct gaaccactct cctgagccat tttccctgtt
      601 cggggattga gtaatgccag cgccttcgca gaccgtcttt gatggctttc agagttgccg
      661 tcatccgatc ccggcgggtc tttcgtatca gcatgaacct gccgttgcga tctttcccgc
      721 tgatgtgcgt gaacccgagg aagttgaacg tttctggttt gccttttccc ctgatggcac
      781 ggttttcggc agcgaagcgg ccgaactcca tcagacgggt tttctccggg tgaaccgtga
      841 gtccgaactc cctcagtctg cgctgcatgg ctatacggaa gcgccgggca tcgtatcgtt
      901 tgtcgaaccc gatgacgatg tcatcggcgt atctgaccat taccacattg cctgtggcat
      961 agcgacgtcg ccactgatgc gcccacagat cgaagacgta gtggaggtat atgtttgcca
     1021 gcagcggtga gatgaccgca ccctgtgggg tgccttcctc cgttgctcgc cattgaccct
     1081 cctccgacgt cccggctgtg agccacttac gtatgagcct gattaccctc cggtcgccga
     1141 tccgatgctc tgtgaacctg atcagccatt cgtggctcac cctgtcgaag aactgactga
     1201 tgtcggcatc cagtacccag tttacgttag tgcgtaccag ccctgtggcc agtgcgtcca
     1261 gtgcatcgtg ctggcttcgc ccgggtctga acccgtatga gaaccccata aagtcgtttt
     1321 catagactgc gttcaggatt ttcaccagcg catactggac gatcttgtcc tccagcgagg
     1381 cgatgccgag cgggcgttgt tttccatccg cttttgggat gtagtgacgc ctgccgggct
     1441 gcgccctgta gctgccctga tgtagcctcc ggtgcagatc tgttatgttg ttcttcatgt
     1501 ttccggcgta gtccatccac ctgatgccat ccactccggc ggccgctttc ctgctcaggg
     1561 agaggaatgc ggcttccagt gcttcgactg tcagcaggtg gaacaatgct gtaaaccgtt
     1621 ctttcttccg ctgcttcgca gcttcccgca cgcgtgacag cctctgtgac atgctttccc
     1681 ggctctgtgt ccggcgcatg tgtggctgtt ccgcgttccc cttggccccg ctccttcgct
     1741 ccactgactc cgctcctttc gggttgttcg cctgcttcgc cgctactatg agcgagtccg
     1801 acttctcctc tccgtacatc accggctatg actcctcgtc ttcccggtgc gggccatctc
     1861 cgacactggc agatggtcag aggggagatc tcccggttcc cgcgtagaga tcgtattgac
     1921 atgccagggt ctcagacccc gccgggtcca tgtggcactc gcagtatcgc accctatgat
     1981 gttgccttcc gttaacagtg caacgtcggc acccggtaat ttaatataca tttcgtggct
     2041 caatggctgg cctgtcaaca cccctgtcaa cgcttcgccc catacctcgc ggtatgcaac
     2101 gcatgactcg gggaccttgt ggattgctgg tccttcaatg gtcggggact ttcacccctt
     2161 gatctctaac cggtctcccg gcgcacactg tacatataca cagtataatt attaccttac
     2221 tcctcccgtt ttcaggtgtc aatatatgag attagttctc atcttcaggc acaaaaaagc
     2281 gcccggagcc acctgtaagc agatcgcgtc ttttcccgaa aatttctcat ggcaacaaat
     2341 tttattattt aagacattca tcacacttac atttcagatg gtatagacac atctgaaagc
     2401 taactggcat gaggagagga cctgcggtta aactgttcgg gaaacctgag acgcccggag
     2461 gccactatga cgcacctgaa actgactccc gctgaagaaa agcgcctggc ggacgccttt
     2521 gtcgctcagg cactggccat gggcctgaaa gggaacaaaa cgctgccaaa ggcggtaaaa
     2581 caacaggtgc gggaaagggt gcttcgtgcc attcaggacg aacgtgaagc caggcgggcg
     2641 caggaaatgc gtcgggagaa atacgaggct acggaaacca ccttcacctg gcagccacag
     2701 cgccgccggt aaggggtatt taccataacg tcgcattgcc cgcaccgctc agacggtctg
     2761 agggcgtttc cggcggctac gggataaaac aggcaacaaa ccacccggaa aaccgccgga
     2821 aacgcgcccg gtaaattgta acccgctgca tgcctatgca cgcaactgca tggcgggccg
     2881 tttggtgtaa aaacgatacg ataagccgtg tttatgtaaa tgaacgtggc cgtaaagagt
     2941 gacaaatata catgtaacat tttttactga caggtcatca acccgatgcg tttaataccc
     3001 tttatcctgc tggcattgtg cagcccctta ctcctgacgg cctgtaagca ttccaccacc
     3061 cagccgccga aaatttatac tctggataag tcatctaaaa tcagtagcta cgctgcggcc
     3121 atcaagaccg ctgttgaaat gcagatgttt gaaccagagg cctatgcggg aaaaatgtgc
     3181 accgtgcgga tatccataca acgtgatggc actctgaaca gcgccattgc tgaaggagga
     3241 gatccgaaac tctgtaaagc agcaatatca gccgttaccc acgccaaaat tccgccggca
     3301 ccggatgaaa atacctggca aatattcaaa aacgcccctc tggatttcag accatgaaag
     3361 cttttactct gacagtgctg gcggccatgt tcctcactcc ggtacatgcg ataccactaa
     3421 aacccgtaac cacactgcag ccaacctacc cggtacaggc cgcagcaaaa cgcatcgaag
     3481 gtcaggtgac cgccacgttt gatattctgg acgatggacg agtggacaac gtgtcgctta
     3541 ccggctgtca cctttttttt cgggaaacgc tgacggcaat gaggcactgg ctatatcccg
     3601 cagggcaccc ggcaatgcga caggttataa attttgaatt cagtctgaaa aatgacggct
     3661 tacaggatcc gcgaccagtc accacccaaa agctcaatga tgatggttgc cgccagtaac
     3721 aggtccttta acccgcaggc tgcaatagtc ctggatgatg agtgagcctg tagcagccct
     3781 gattagtgga agcgaagctg aaaaatgggc agcaaaagcc gtatttctgg caaatagcgc
     3841 cgccacggat taaagtagtc atgtgtaata catatgacta caaaggggct tttatgtcat
     3901 cagcaacatc catcaggctc gatgaagaac ttaaggaccg gctgaaaacg ctggccgatg
     3961 accggcaccg ctcagcgcac gcgctaatgc tggaggcaat caccgaatac atcgaccggg
     4021 aagagaagcg cagccagtac ctgcgggacg gacaggccgc atggcagcac tatcaggaaa
     4081 cgggtctgca ccttactgcg gaagaagcgg aagcctggat aagtacctgg ggaactgaga
     4141 acgagcagga tgctccgcca tgccacaggt aacgatttcc gcgctggcac agcgcgattt
     4201 acaacgcctt caggactttc tcaaaaccaa aaatcggctg gcagccagaa aaggaggtga
     4261 ggtgatcgtc cgggctatcc agcaactgaa aacattgcca gacattggcc gcccggtgcc
     4321 gtttctgccg ctggaatata aggaactggt gatcgggttt ggcgacagtg gctatgtgat
     4381 gctctaccgc cacgaccggg aaatggacca gattgtgatc gtcaccgtca ggcatcagaa
     4441 agaatccggg tatccggggg cagacagcct ctaataaccg atgacggggg cgcaattcat
     4501 tgcgtggccg tcgccggttt gacaagcgca gcgcgtcaga aaaccacatc ctgaacggaa
     4561 aaacgggtaa aattcagcga tacatttacc gtgagaaatt acacaccttt cacacccgat
     4621 aaaatcaggg gtatacagtg gcgaaaacat tgctcaacct ttcacaggca gcacaagccg
     4681 cgggcattac cagaagaacg ctatacaatc atgtaaaaca gggaaaggtt accgtgtcac
     4741 gtgacgggaa aaacaaccct gtcgtggatg tgtcagaact gatccgtgta tacggaaatg
     4801 tgaatatccc tgagaaacaa attcccggca tttcacaccg tgaaaataca caaaaaaatt
     4861 tcccacagga acagcttctg gcaatgcaaa aggaactggc cgatctccgg caggctgtca
     4921 ccctgatgct ggaagataaa acatcccggg aagaagaacg ccgccagcac gatgatgagc
     4981 gccggaaact ccaggccgaa gtggacagat tgacaacaga actgacacag aagaaaaaac
     5041 gcttctggtc cggctggttt agctgagaac ttaccgaagg gaatggagaa catgacgaag
     5101 caggaaaaaa ctgccctcaa tatggctaaa ttcattaagg atcagtcgct gctgttgctg
     5161 gaaaagctga acgagctgga catggatacc ccggccgaca tgtgcgaacg gcttcacgag
     5221 gatgcggaaa cactctggca ggtgatgcgg gataagctcg gggaagaata acagcaccgg
     5281 ccatgccggt gctggcgtta ttttttgccg aacggccacc accggcgggc gggtttgtcc
     5341 tccggcagtt caggcaccgg cacgttaaaa tagcggctgt tatattcccg catcagctcc
     5401 gctttcagct tctcgttcac cgcgctttcc ccgtgccgct cgctgaccac ctcaatcatc
     5461 gcgttaatct gctccggtga gcacttcccg gcatcaacca cctgcacacc gttctcgatg
     5521 gcgaaccgct ccacctttcg atagaggtaa tcacacagct tcattcctgt gggtttgtcc
     5581 cgttcccatt gcggattttc accggtgaaa aagtacgtgg ctttttcgtc gctgttgagc
     5641 cgctcgtcat acatcccgat ggatttcatc atcgccagat gctgggcccg gtcgacaaaa
     5701 cagaaaacat acgccccggt ctgccaggcg agcaccacca tgtcttccag atgctcgctg
     5761 gttggcataa tgcagcgctc gccgtctttg tcttccacgt cctcaataaa taccatgtac
     5821 agcgccataa tccctcactc cgttgtcata aatttaaagc acgcgaacag cctctcccgg
     5881 tccagctcca ccgttttgcg ggcctcgccg gtctgtggat tcacgatttc atccaccgcc
     5941 agaatcgccc gctgtacccg gacaagcgtc ggccggtcag ggcagatgta ccagatttca
     6001 tcgatgccgt tcgcccggcg gttgaacaga tacccggaca ccacttcctg ataccgcctg
     6061 cggctcttga cggtgcgctc tacctcaaat gccaccgttc tgccgtccag ctccgccagc
     6121 gcgtccggca ctttcatccc tttcagggcc atacggtgca ggtaccgcca gccggtggcc
     6181 ccccggccct ccatggagag gcgcagggac tgcacggcca gccggtgcgg cagctgcgcc
     6241 gccgacaccc ttgacggctg aaacggaata acgtccgtca gcggctcgtc ctcgtcgaac
     6301 gagaacgcca ccccgtgcgg cgtcagcccc cacacgctga cctgacggcc gtcaaccggg
     6361 tatttcgccc ggaccagcag ccctttcgcc accagactgt ccaggaagcg gtaaaccggc
     6421 tgtaccgccg caatccccag ccgtcgcgca accagctccg ccgtggaaca cagctcttcc
     6481 cgcagccagc gcagggtcag ccgtgttttc tccgcgttcc gcgcctgccg cttcgcgtgg
     6541 ctgacgttgt actgctccag tgtcatcagt aaagccccgc aaaatcatca tccgcttttg
     6601 gtccgccgcc gtcggccgga ggcaggtact ccgccagcgg gtccgttttc gccagcaccg
     6661 ggaaggtttt cagcgaaatt tcccggcgct caacccgcag cgggctgctg aacgccagcc
     6721 gcgccacgcc cagcccggtc atcaccgcga acccgtcagg caggtgttgc agggtgttca
     6781 cgtcaaacag cggccgcgcc tccttctgga tgaagctgtc gccgctgatg tgctccacgt
     6841 tccccgcctc ccggctggcc cggcggcgct ccacgtccac ccggatctcc ccggtctggc
     6901 ccgccgccca ctgcgcggac tcctggctgg ccgcgcgata gaaccagcgg atcggcgtgt
     6961 tatccagtac cgtggttttc acaaaatcag cgggcagatc ctgcccgcac tgaccgaagt
     7021 cgcccagcga ctggtgcgcg agcagcaggt tgcagtcccg gctgcgcagc gtccccagcg
     7081 cgttcagcac gtacttcgac agcagatatt taatctcatc gagcatgatg ctcgcgtgcg
     7141 gccacctccg gaactcatcc cgcgcaatga tgatttgcgc gcagcgggca aacagcatct
     7201 tctgcacccg gatcaccgcc tcatcgtcca tcgagccgat gacgtacagg cagccgccgc
     7261 cgttgattat ccccgccaca tcgataccgc tgtccgtctg caggacgctc aggtcggcca
     7321 cgttctccag cttggtgatc agccccttca cgtccttttt cagggcctcc ggcaacgcgt
     7381 acgccgccgc cagcacctgc tgaatgtttg ccccctgcgg gaactgctcc gcaatcagct
     7441 tcgccgcttt ctgctcgctg atgcggtagt ggtccgccac gtcgccggtc tcactcaggt
     7501 taaacccggc aaccagcaga ttcttcaggg cgtactggtc gagatcgcgg aacagattca
     7561 gctgcgccgg tttccccggc cgcagatcca gcagggtgaa gtccgggcag tgcgcccgga
     7621 agacgtgcgg cgcccacgcg tcccctttgg ggtcgaagta caccacagcg tccccgaaat
     7681 cgcgtacgca gcgaatgagc gcgttggtgg ccatcgcgct cttgccgctc ccgggcaggc
     7741 cgaggatctg aatattggtc ttgcgccagg tatcacgagg gaccagcacc ggctggccct
     7801 gctcatcctt acccagaaac accgcctgct gtttccgggc ctgctccgtg gaaaaatcgg
     7861 ccagcgcgtt aaccggtacc ggctccccga aatgcaggtg gcggacgtct gtcatttctc
     7921 cggtccgctg gctgctgcgc cggctctcct gattcagttt cgcgttcacc gcctcgtcag
     7981 cggccgcaaa atgaccatca agcagcagga acgccagcag ccgcgccaga aggacgccgg
     8041 cgacaagccc tccggcacac caggctatcc cgaacaggtt agccggctgc agcaggtaat
     8101 aatctgcgtt cgcatagagg tccgggcgca ggacggtttc tccccagctc tcgataaaca
     8161 cggcgacaac cagccccccc agaagggcca gaccggcagc gccggtcagc cagtacgcgt
     8221 agttccagac atgtcgccgt gccctgacca tccgcccggg tgaatccagc gaggccagca
     8281 ccgaaaatgg cacagtccgg ttattatcgc gcttatagcg atcggcatac tcccgccgcg
     8341 ccttatggct cagccccaca aaacgcattc ggccggccca gagttcagcc agaccgaaaa
     8401 acaggccgat aaccagaaaa aaaccggcca gactgtaata ctcccggtac agccagctga
     8461 cccacgggta aatctgccgg gccagctccg ggatatgcca ctgccaggca agcgaatccg
     8521 tattaaacat gcaatctctc tccttcagac gacgggttta ccggcgtgat gcgcagcccc
     8581 cgctgcacaa gccgcggaca tgccccaagg catggcggcg gcggtcacgt ccgtggtgat
     8641 tgcgttcagc tttcaccgcg gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc
     8701 cccggaggaa caacgagcac gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg
     8761 tcttttccgg tgttccggcc cccgtcagag ttcgccatag cgagcggctg accacccgca
     8821 aaatcaggag cagctttgct gcttcgtcag gtgcgtccag caccgtggat ttttgtgggt
     8881 ggtcaggcgt gagcctgacg ggggccgggt gaatttctgg cgtggccggc gtccagccgg
     8941 acagagccag aaagagggga aaagaccgtg cgtcggtggc gtccagccag cgtaagcaag
     9001 gtcttttccc ctgaagtcag ccacgcccca gcggggatga cttgcatttc tccgcaagga
     9061 gaaaaaagca acagagatag gttaacgtaa aatgttcagc gaaaaaccga catggttcac
     9121 ctatcctgat aattgatcgt cagggaatag aaagacttaa tcaggggaca atatcccaca
     9181 tcagcgcgtc cggtgaccgt cttgcaggtg ccggggaaat tgcgcctcct cgctcactcg
     9241 gtcgctacgc tccgggcgtg agactgcggc gggcgctgcg gacacataca aagttaccca
     9301 cagattccgt ggataagcag gggactaaca tgtgaggcaa aacggcaggg ccgcgccggt
     9361 ggcgtttttc cataggctcc gccctcctgc cagagttcac ataaacagac gctttttcgg
     9421 tgcatctgtg ggagccgtga ggctcaacca tgaatctgac agtacgggcg aaacccgaca
     9481 ggacttaaag atccccaccg tttccggtag gtcgctccct cttgcgctct cctgttccga
     9541 ccctgccgtt taccggatac ctgtccgccg ttctcccttg cgggaagtgt ggcgctttct
     9601 catagctcac acactggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta
     9661 agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt
     9721 ccaacccggg aaagcacgca aaacgccact ggcagcagcc attggtaact gggagttcgc
     9781 agaggatttg tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac
     9841 actggaagga cagatttggt tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt
     9901 tccccaactg acttaacctt cgatcaaacc acctccccag gtggtttttt cgtttacagg
     9961 gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctactga
    10021 accgcgatcc ccgtcagttc agaggaggac gatggtgcca ccgttccgcc ctgaacatcc
    10081 cggatataat ttgcctgaca tgcggccaga agaaagccag caacaatcaa tataaaaaga
    10141 aaaatagtct ttaccttttt catatacttt ccctgtattt aaagactccg gcattcatcc
    10201 ctgaacaccg gaagtcagta tttcaaatca tccttgataa ttttttgtta ccagttaccg
    10261 tcccgataat caaaagctat ataacaatta tccacatcag gttttatttg atttttaaaa
    10321 aatggctgta gtaaaggaac ccattctttc ttcaaatcaa actcaccatt attaatatta
    10381 tcctttaaag gcattcccaa gctttcaatg acagaaccat cgtcaccaaa gtctttagaa
    10441 tactcttccc ctttaaactc ttcagttttc ttatcaaacc atgtcaaacg taatttaagc
    10501 cccatattta cctctcaaag atatttttta atgttccgtt ttggatctgg ccctttaacc
    10561 tgcttacccg ttttgggatc aaatgcgcct aggtgttcgc catcactggc acgataccct
    10621 tcaagctcac cgtgctggga atcccattca taaattttac gttttttatc gccataccat
    10681 cgggcccgct taccaccacc accttgttta ggtgttttag gatcacctcg ctttaactct
    10741 ccaagtccct ttatgtcttc tgttttaggt actggatgat aatcatggcc gtagtcttta
    10801 gtccccttgc gaggcttgtt cttttcctca tttaacttat tttctgcatc cttcgctttt
    10861 ttctcctttt gtttccggct ctccatcgcc ccggtaagtg cagccttatc tctggataag
    10921 gattcattca ttgcgtttaa agatgcctga gccgctttta atttctcatt ctgattctga
    10981 atatccttat caagatagcg aacctgatcc tgccacccgg agccatattc atgaggcggg
    11041 ttataagtca caaaatcagg taatgactcc aatttactga tagtgtttta tgttcagata
    11101 gtgcccgatg actttgtcat gcagctccac cgattttgag aacgacaggg acttcctgcc
    11161 cagccttgcc agatgctgtc tcagattcag gttgtgtcgc tcaattcgct gcgtgtaacg
    11221 cttactgatg acgtgcagtt ttcccttcag acgtgactca tacatcggcc agccgtccgt
    11281 catccatacc accacgtcaa aaactgacag caggctcaga agacgctcca gtgtggccat
    11341 agtgcgttca ccgaaaacgt gggctaccac cgccctgcgt atcctgtcat acgcgtaaaa
    11401 cagccagcgc tgacgtgatt ttgcaccgac gtagccccat tgctcgtcca tctccgcaca
    11461 gacaatgaca tcactgcccg gctgtatccg ggaggttact gactgcggcc tgagtttttt
    11521 aaatggcgca aaatggtgtt gaggcccacg cccattagtc gtgcggtggc acggcaaccg
    11581 acgccgttca tagccatatc aatgattttc tggtgtgtac cgggctgaga agcggcataa
    11641 gtgaaggtga gctgccatgt tttacggcag tgagagcaga gataacgttg atgcccggca
    11701 gtacttttgc cattacgcat cacgccttca gtagcggaac aggagggaca gcagacggag
    11761 actgaagcca cgggagcacc tcaaaaacac cattatacac taaatcagca agttggtagc
    11821 atcacccaaa atcatcttta tttgcctctg cctcttttac ctttttatcg gcttcctgaa
    11881 cagcagccct tgcaccgggg agcgccttct ctgtcgatgc aatgcggctg tttaaggtcg
    11941 caatattttt atcttcggca tccagctcag ctttcgcttt atcatagtct cttttcagcc
    12001 cctcttccgg atgagcggcg tcccatgcct gctggcgacg cttttcttcc tcctgacgct
    12061 gttttacctg tgccggggta agaacatctg tcaccgacac ataaaccggc ttctgtccgg
    12121 tctctttcgg gaaacgaata acggcctcac gggagttacc accggctgta aaaccagccg
    12181 gacgtgaatc acctttttca gcaacaatgc cttttggcgg ggctttcgct gtcggaacac
    12241 ctttcggtac gctcacctgc agagacggga gacccggaat cgacacactg aatacccccg
    12301 gacgttttgt cggtttcgca tccaccacag ggacactcat tggccgacct gcgacaaccg
    12361 caatatgctg ccgctcatcc ttcaccacat ccacaacccg ttgtctgaca cgaaccgtcg
    12421 cctggtccag tggtaaagta cttgccggag tctccgtcac cgcatcggcc ggtaatgacg
    12481 tcataatttt tgacatcatt ttcggatcat cttttgcaat ctcagaaggc agcacaccgt
    12541 agatcgcaat cccccacaga ccaaacttaa acggcccctt cagggcagcc agcacatcag
    12601 caacggcggc cgacaacgca tcgccggaaa ctgataaagc cagtccttca gcccccggag
    12661 tagccagggc aggcagaccg aaggccatcg cagtggccga agactgtccg ccaccagagc
    12721 taccgctatg attgccgctg ttacca
//
