LOCUS       NZ_CP046652             3511 bp    DNA     circular CON 23-DEC-2023
DEFINITION  Klebsiella pneumoniae strain EGKP3 plasmid pEGKP3.8, complete
            sequence.
ACCESSION   NZ_CP046652
VERSION     NZ_CP046652.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13497075
            Assembly: GCF_017161135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Hamza,E., Dona,V., Elhariri,M., Elhelw,R., Hamza,D. and Perreten,V.
  TITLE     WGS of K. pneumoniae producing carbapenamese from Egypt
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Hamza,E., Dona,V. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2019) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: DEC-2019
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            NovaSeq 6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/23/2023 20:08:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,683
            CDSs (total)                      :: 5,562
            Genes (coding)                    :: 5,414
            CDSs (with protein)               :: 5,414
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 64 of 148
            Pseudo Genes (incomplete)         :: 86 of 148
            Pseudo Genes (internal stop)      :: 24 of 148
            Pseudo Genes (multiple problems)  :: 22 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046652.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="EGKP3"
                     /isolation_source="fecal isolate from poultry farm worker"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pEGKP3.8"
                     /geo_loc_name="Egypt"
                     /lat_lon="30.0131 N 31.2089 E"
                     /collection_date="2015"
                     /collected_by="Veterinarian"
     gene            373..555
                     /locus_tag="GPU01_RS28360"
                     /old_locus_tag="GPU01_27925"
     CDS             373..555
                     /locus_tag="GPU01_RS28360"
                     /old_locus_tag="GPU01_27925"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016265984.1"
                     /translation="MLTFIGVVVVAVIAFKLFMLFFSSSESAVERATRKYMENPTSANY
                     KIMVATRMRNEKKRG"
                     /name="hypothetical protein"
     gene            1751..2092
                     /locus_tag="GPU01_RS28365"
                     /old_locus_tag="GPU01_27930"
     CDS             1751..2092
                     /locus_tag="GPU01_RS28365"
                     /old_locus_tag="GPU01_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2301..>2729
                     /locus_tag="GPU01_RS28370"
                     /pseudo=""
     CDS             2301..>2729
                     /locus_tag="GPU01_RS28370"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2770..3267
                     /locus_tag="GPU01_RS28375"
                     /old_locus_tag="GPU01_27940"
     CDS             2770..3267
                     /locus_tag="GPU01_RS28375"
                     /old_locus_tag="GPU01_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            3274..3489
                     /locus_tag="GPU01_RS28380"
                     /old_locus_tag="GPU01_27945"
     CDS             3274..3489
                     /locus_tag="GPU01_RS28380"
                     /old_locus_tag="GPU01_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1493..1666
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(1005..1526)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.69"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2083..3509
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgggcaaaca acggaaaagg aaccagagaa gacagcagat ctggtaccgg aacggcaacg
       61 cacattatct catggtgaca gggagtatgg agactgataa acgcccgtct caggtggcct
      121 gagcgtgacg gggatcatgg ccattttctg cgatttcagc acacagagag tcttttttaa
      181 ccgaaatcac cggctatgca gtgatgtgaa taagtgatgc atatgcgtaa aataaatcag
      241 gtgcgtttct ggtggttttc ggtgtacttg ccaccacctt ttgcccgtca gccatcagaa
      301 acgccctgag tgcctccggc gcggtgcggg taagagatgt tatgtttaat gttgataaca
      361 ggagtaagag tgatgcttac atttattggt gtagtggtag tcgccgttat tgcatttaaa
      421 ttatttatgc ttttttttag ttcatccgaa tctgccgtcg aacgtgctac ccgtaaatat
      481 atggagaacc ctacgagtgc taactataaa ataatggtgg ctacacgcat gagaaatgag
      541 aagaaaagag ggtgatatat gtttgagttt attggttttt tatttttact tttggtgtgt
      601 tatgtcttta tacaaatggt tgctgtcagg gtgtttcctg aatatggagt acggaaagcg
      661 gaggaacgat accgtcgaaa tccatcagca gaaaatgagg atctgttatg gagagctaga
      721 agtcgtataa ctaaacgcaa gtaatgtttc ctaagtgtgt tgataggcaa acatggtggg
      781 agttactact ctcggagtta atgatcttct tgtgatcctg tcgtttcagt cgtaatctct
      841 tgctctgaaa acgaaaaaac cacctgggga ggtggtttga tcgaaggtta agtcagttgg
      901 ggaactgctt aaccaggtaa ctgggtatac gagaccgcag ataccaaaac tgttctttca
      961 gtgtagccgc agtcaggccc atacttcaag acctctgaat ctctctctcg tatatcccag
     1021 ttaccagtgg ctgctgccag tggcgctttg tcgtgctttc tcgggttgga ctcaagatga
     1081 tagttaccgg gtaaggcgca gcagtcggac tgaacggggg gtttgtgcat acagtccagc
     1141 ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg gaatgagata aacgcggcca
     1201 taacagcgga atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc
     1261 gccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga
     1321 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gcttcgccgc
     1381 ggccctatcg cttccctgtt aagtatcttc ctggcatctt ccaggaaatc tccgccccgt
     1441 tcgtaagcca tttccgctcg ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag
     1501 gaagcggaat atatcctgta tcacatattc tgctgacgcg ccggtgccgc cttttttctc
     1561 ctgccacatg aagcacttca ctgacatcct catccgtgcc aacatagtaa gccagtatac
     1621 actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaaccccac tgacgcgcct
     1681 gcggcttgtc caaccccgcg ccgaccggga aaggctttcc cgtccgtcac ggcgttatca
     1741 ggaggcgatt ctggcagata aacgcaacaa gatgctcacg atgtgggtga cggaagacga
     1801 acaccggcgg ttgcttgagc ggtgcgacgg aaaacaactg gcggcgtgga tgcgccagac
     1861 ctgccttgat gagaaaccga cacgcgccgg caggcttccc tcgatttcgc ccgcgctgct
     1921 gcgtcagctt gccggcatgg gcaataacct gaaccagatc gcccggaaag tgaacgccgg
     1981 tggtgccggt catgaccggg tgcagatcgt ggcagcgctg atggcgatcg atgccggtct
     2041 ggaacggttg cggcatgccg tactggaaaa agggccggac gatgattgtt aaattccatc
     2101 cccggggtcg cggcgggggc gccggtcctg tggattatct tctgggtaaa gaccggcagc
     2161 gtgaaggtgc cagcgtcctg caggggaagc cggaagaagt ccgggagctg attgatgcgt
     2221 cgccgtatgt gaaaaaatat acctccgggg tgctgtcatt cgcggaggcg gacctgccgc
     2281 cgggtcagcg tgaaaaactg atggccagct ttgagcgggt tctgatgccc ggactcgata
     2341 aagaccagta cagcattctg tgggtggaac acgcagacaa gggtcgtctt gagctgaatt
     2401 ttctgatccc caataccgaa ttactgacgg ggaaacgcct ccagccgtat tacgatcgtg
     2461 ccgaccggcc gcgcatcgat gcgtggcaga cagtggtcaa cggcaggctg gggctacatg
     2521 atccgaacga cccggagaac cggcgggcgc tggtcacgcc ttcaggactg ccagaaacga
     2581 agcaggaagc cgcagagacg attacacggg gtttgctgat gttagcctca tcaggagagc
     2641 tgaaaacgcg tgatgacgtc acaggggcgc tcacagcggc cggttttgag gtggtcagaa
     2701 ccacaaaaaa cagtatcagc attgccgacc cggacggggg gcgaaatatc cgactgaggg
     2761 gagccattta tgagcagtct tttaacggcc tcgagggact tcgaggagaa atcgaaagca
     2821 cagcagcagc ttaccgaaga gaggctgaaa gccgcattca gcaagcacga gaacgttgtc
     2881 aaatcggaac tgaacgcaag cgcgaagaga atcaacgacg ccatcagcgc ccacgaacag
     2941 gggatgaacg cggcgatgca gtcgaaccgg ctgagcgtgc tgcgaatggt cgggcggacg
     3001 tggctgacca tcaccatggt gtcagggctg ctgttcgcca gcctgagcgg ggtgctgtgg
     3061 taccagggga accggatagc ggcgaacctg gcggaaatca ggcagcagag agatacgctg
     3121 tcgaaactgc acatgcagac gtggggcgtg acctatctgg agaaccgcaa cggacggttt
     3181 ctggtgctgc cggaggggat gaaagccgaa acgggccgga cggtggacaa caagacgcgg
     3241 aacgcggtga agctggtacg ggagtagagc agaatgacgg aactggaaaa acaattgctg
     3301 agcgcgttag agcagctaca gcaggactac tcgagaaggc tggacgagtg ggagagcgcc
     3361 ttcgaggaat ggcggaagat gtctggtctt atgcaacggg agaacgcggc gctgagcgag
     3421 cgcgtgtcgg gcttgagcag gcaggtgcag agtttgagcg agcagctgcg ccggttatcg
     3481 acaggctgaa tgagattgag caacagcgat a
//
