LOCUS       NZ_CP046650             9294 bp    DNA     circular CON 23-DEC-2023
DEFINITION  Klebsiella pneumoniae strain EGKP3 plasmid pEGKP3.6, complete
            sequence.
ACCESSION   NZ_CP046650
VERSION     NZ_CP046650.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13497075
            Assembly: GCF_017161135.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9294)
  AUTHORS   Hamza,E., Dona,V., Elhariri,M., Elhelw,R., Hamza,D. and Perreten,V.
  TITLE     WGS of K. pneumoniae producing carbapenamese from Egypt
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9294)
  AUTHORS   Hamza,E., Dona,V. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2019) Institute of Veterinary Bacteriology,
            University of Bern, Laenggassstrasse 122, Bern 3012, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: DEC-2019
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MiniION and Illumina
            NovaSeq 6000
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/23/2023 20:08:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,683
            CDSs (total)                      :: 5,562
            Genes (coding)                    :: 5,414
            CDSs (with protein)               :: 5,414
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 148
            CDSs (without protein)            :: 148
            Pseudo Genes (ambiguous residues) :: 0 of 148
            Pseudo Genes (frameshifted)       :: 64 of 148
            Pseudo Genes (incomplete)         :: 86 of 148
            Pseudo Genes (internal stop)      :: 24 of 148
            Pseudo Genes (multiple problems)  :: 22 of 148
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046650.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9294
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="EGKP3"
                     /isolation_source="fecal isolate from poultry farm worker"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pEGKP3.6"
                     /geo_loc_name="Egypt"
                     /lat_lon="30.0131 N 31.2089 E"
                     /collection_date="2015"
                     /collected_by="Veterinarian"
     gene            1241..3205
                     /locus_tag="GPU01_RS28275"
                     /old_locus_tag="GPU01_27845"
     CDS             1241..3205
                     /locus_tag="GPU01_RS28275"
                     /old_locus_tag="GPU01_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            3205..3936
                     /locus_tag="GPU01_RS28280"
                     /old_locus_tag="GPU01_27850"
     CDS             3205..3936
                     /locus_tag="GPU01_RS28280"
                     /old_locus_tag="GPU01_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            3943..4473
                     /locus_tag="GPU01_RS28285"
                     /old_locus_tag="GPU01_27855"
     CDS             3943..4473
                     /locus_tag="GPU01_RS28285"
                     /old_locus_tag="GPU01_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(4501..4680)
                     /locus_tag="GPU01_RS28290"
                     /old_locus_tag="GPU01_27860"
     CDS             complement(4501..4680)
                     /locus_tag="GPU01_RS28290"
                     /old_locus_tag="GPU01_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4749..5126
                     /locus_tag="GPU01_RS28295"
                     /old_locus_tag="GPU01_27865"
     CDS             4749..5126
                     /locus_tag="GPU01_RS28295"
                     /old_locus_tag="GPU01_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(5188..5487)
                     /locus_tag="GPU01_RS28300"
                     /old_locus_tag="GPU01_27870"
     CDS             complement(5188..5487)
                     /locus_tag="GPU01_RS28300"
                     /old_locus_tag="GPU01_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5477..5740)
                     /locus_tag="GPU01_RS28305"
                     /old_locus_tag="GPU01_27875"
     CDS             complement(5477..5740)
                     /locus_tag="GPU01_RS28305"
                     /old_locus_tag="GPU01_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(6046..6465)
                     /locus_tag="GPU01_RS28310"
                     /old_locus_tag="GPU01_27880"
     CDS             complement(6046..6465)
                     /locus_tag="GPU01_RS28310"
                     /old_locus_tag="GPU01_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            6868..8553
                     /locus_tag="GPU01_RS28315"
                     /old_locus_tag="GPU01_27885"
     CDS             6868..8553
                     /locus_tag="GPU01_RS28315"
                     /old_locus_tag="GPU01_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8563..8820
                     /locus_tag="GPU01_RS28320"
                     /old_locus_tag="GPU01_27890"
     CDS             8563..8820
                     /locus_tag="GPU01_RS28320"
                     /old_locus_tag="GPU01_27890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            8905..9054
                     /locus_tag="GPU01_RS28325"
                     /old_locus_tag="GPU01_27895"
     CDS             8905..9054
                     /locus_tag="GPU01_RS28325"
                     /old_locus_tag="GPU01_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1868..9294
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2..442)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.919"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3206..3933
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 cccagttacc aatggctgct gccagtggcg ttttgcgtgc ttttccgggt tggactcaag
       61 tgaacagtta ccggataagg cgcagcagtc gggctgaacg gggggttctt gcttacagcc
      121 cagcttggag cgaacgacct acaccgagcc gagataccag agtgtgagct atgagaaagc
      181 gccacacttc ccgcaaggga gaaaggcgga acaggtatcc gggaaacggc agggttggaa
      241 caggagagcg caagagggag cgattcatcg gaaacggtga tgatctttaa gtcctgtcgg
      301 gtttcgccac tcctgactga ttcatggttg agccaccggc tcccacagat gcaccgaaaa
      361 agcgtctgtt tatgtgaact cagtcaggag ggcggagcct atggaaaaac gccacctgcg
      421 tggccttgcc gttttgcctc acatgttagt cccctgctta ttcacggaat ctgtgggtaa
      481 ctttgcatgt gtccgcagcg cccgccgcag tctcacggcc ggagcgtagc gaccgagtga
      541 gcgaggaggc gcaatttccc cggcacctac aagacggaca ccggacgcgc tgatgtggca
      601 tattgtcccc tgattaagtc tttatattcc ctgataataa attaccagga taggtgaatc
      661 atatcggttt ttcgctgaac attttacgtt aacctatctc tgttgctttt ttctccttac
      721 ggagaaatac aggtcatccc cgcgagggcg tggctgacct cagggggata tcccctgctt
      781 acgctggctg gacgccaccg acgcacggtc tctccccctc tttctggctc cgtccggctg
      841 gacgccggcc acgccagaaa ttcacccggc ccccgtcagg ctcacgcctg accacccaca
      901 aaaatccacg gtgctggacg cacctggcga agcagcaaag ctgctcctga ttttgcgggt
      961 ggtcagccgc tcgctatggc gaactctgac gggggccgga acaccggaaa agaccgcact
     1021 ggggtgccgc cttttcctct cacacgctgg ggcgtgctcg ttgttcctcc ggggctgcgc
     1081 cttttccgca tggggcggaa agaggctggc ctccgtggtg aaagctgaac gcaatcacca
     1141 cggacgtgac cgccgccgcc atgccttggg gcatgtccgc ggcttgtgca gcgggggctg
     1201 cacatcacgc cggcaaacca tcgtctgaag gagagagaac atgtttaata cggattcgct
     1261 tgcctggaag tggcacattc cggagctggc ccggcagatt tatccgtggg tcagctggct
     1321 gtaccaggag tattacggtc tggccggggc tttcctagtt atcggcctgt ttttcggtct
     1381 ggctgaactt tgggccggac ggatgcgttt tgtggggctg agtcataagg agcgacggga
     1441 gtatgccgat cgctataagc gcgataataa acggccagcg ccattttcgg tgctggcctc
     1501 gctggattcg cccgggcgga tggtcagggc acgtcgacat gtctggaact acgcgtactg
     1561 gctgaccggc gcggccggtc tggcccttct gggggggctg gttgtcgccg tgtttatcga
     1621 gagctgggga gaaaccgtcc tgcgtccgga cctctacgca aacgcagatt attacctgct
     1681 gcagccggct aacctgttcg ggattgcctg gtgcgcgggc gggctggctg tcgggattgt
     1741 gttggcgcgg ctgctggcgt tcctgctgct tgacggtcat tttgcggctg ctgacgaggc
     1801 ggtgaacgcg aaactgaatc aggagagccg gcgcagcagc cagcggacag gagagatgac
     1861 cgacgtgcgc cacctgcatt tcggggagcc ggtaccggtt aatgcgctgg ccgatttttc
     1921 ctcggagcag gcccggcagg agcaggcggt gtttctgggt aaggatgagc agggcaagcc
     1981 ggtgctggtt ccgcgtgata cctggcgcaa gaccaacatt cagatcctcg gcctgcccgg
     2041 gagcggcaag agcgtgatgg ccaccaacgc gctcattcgc tgcgtacgcg atttcgggga
     2101 cgcggtggtg tacttcgacc ccaaagggga cgcatgggcg ccgcacgtct tccgggcgca
     2161 ctgtccggac ttcaccctgc tggatctgcg gccggggaaa ccggcgcagc tgaatctgtt
     2221 ccgcgatctc gaccagtacg cgctgaaaaa cctgctgatt gccgggctta acctgggcga
     2281 gaccggcgac gtggcggacc actaccgcat cagcgagcag aaagcggcga agctgattgc
     2341 ggagcagttt ccgcaggggg caaacattca gcaggtgctg gcggcggcgt acgcgctgcc
     2401 ggaggccctg aaaaaggacg tgaaggggtt gatcactagg ctggaaaacg tcgctgacct
     2461 gagcgtcctg cagacggaca gtggcatcga tgtggcgggg attatcaacg gcggcggctg
     2521 cctgtacgtt atcggctcga tggacgacga ggcggtgatc cgtgtgcaaa aaatgctgtt
     2581 tgcccgctgc gcgcaaatca tcattgcgcg ggacgagttc cgggagtggc cacacgtcag
     2641 cgtgatgctc gatgaaatta aatatctgct gtcgaagtat gtactgaacg cactggggac
     2701 gctgcgcagc cgggactgca atctgttgct cgcgcaccag tcgctgggcg atttcgggca
     2761 gtgcgggcag gatctgcccg ctgaatttgt gaaaaccacg gtactggata acacgccgat
     2821 ccgctggttt taccgggcgg ccagcctgga gtccgcgcag tgggcggcgg ggcagaccgg
     2881 ggaaatcagg gtggacgtgg agcgccgccg tgccagccgg gaggcgggga acgtggagca
     2941 tatcagcggg gacagcttca tccagaagga ggcgcggccg ctgtttgacg tgaacaccct
     3001 gcaacacctg ccggacgggt tcgcggtcat gaccgggctg ggtgtggccc ggatggcgtt
     3061 cagcagtccg ctgcgggttg agcgccggga aatcccgttg cagcgtttcc cggtgctggc
     3121 gaaaaccgat cccctggcgg agttccggcc cccggccgac ggcggcgggc cgaaagcgga
     3181 tgatgatttt gcggggattt actgatggcg ctggagctgt acaacgtcag ccacgcgaaa
     3241 cgacaggcgc ggaacgcgga gaaaacccgg ctgaccctgc gctggttgcg ggaagagctg
     3301 tgttccacgg cggagctggt tgcgcggcgg ctggggattg cggcggtgca gccggtctac
     3361 cgcttcctcg acagcctggt ggcgaaaggg ctgctggtcc ggaccaaata tccggtggac
     3421 ggccgtcagg tcagcgtgtg ggggctgacg ccgcatggcg tggcgttctc gttcgacgag
     3481 gacgaaccgc tggcggatgt tattccgttt cagccgtcaa gggtgtcggc agcacatctg
     3541 ccgcaccggc tggccgtgca atccctgcgc cttgatatgg aagccagagg ggccaccggc
     3601 tggcgcattc tgcaccgtct gagtcttaaa ggcatgaaag tgccggacgc cctggcggag
     3661 ctggacggta aaacggtggc gtttgaggtg gagcgcaccg tcaagagccg tcgccggtat
     3721 caggaagtgg tgtccgggta tctgttcaac cgcaaagcta acggcatcga tgaaatctgg
     3781 tacatctgcc cggaccgggc gacgcaggtc agggtgcagc gggcaatcct gtcggtggat
     3841 gaaatcgtga acccacagac cggcgaggcc cgcaaaacgg cagagctgga tcgtgaacgg
     3901 ttgtttgcgt gctttaaatt tatgacaacg gagtgaggga ttatggcgct gtacatggta
     3961 tttattgaag acgcggagga ccggaacggc gagcgctgca tcatgccgac cagcgagcat
     4021 ctggaagata tggtggtgct cgcctggcag accggggcgt atgttttctg ttttgtcgac
     4081 cgggcccagc atctggcgat gatgaaatcc atcgggatgt atgacgagcg gctcaacagc
     4141 gacgaaaaag ccacgtactt tttcactggt aaaaatccgc aatgggaacg gaacaaaccc
     4201 acaggaatga agctgtgtga ttacctctat cgcaaggtgg agcggttcgc catcgagaac
     4261 ggtgtgcagg tggttgatgc cgggaagtgc tcaccggagc agattaacgc gatgattgac
     4321 gtggtgagtg agcggcacgg ggaaagcgcg gtgaacgaga agctgaaagc ggagctgatg
     4381 cgggaatata acagccgcta ttttaacgtg ccggtacctg aactgccgga ggacaaacct
     4441 gcccgccggt ggtggccgtt cggcaaaaaa taaatgccag caccggccat gccggtgctg
     4501 ttattcctcc ccgagcttat cccgcatcac ctgccagagt gtttccgcat cctcgtgtag
     4561 ccgctcgcac atgtcggccg gggtatccat atccagctca ttcagtttat cgagcaatag
     4621 cagcgactgc tccttaataa atttagccat attgagggct gttttttcct gttttgtcat
     4681 gattctttcc ttccgggcat ggtgtgcgcc tgtcaggtat actgtctcaa aaatagcggg
     4741 gtgagacaat ggcgtgggtg acggtgagac aggcgacgga actgacgggt aaagcccgaa
     4801 gttccctgta tcgggatatg gcgaaaggcc gcgtatcata ccgtacggaa gctgatggcg
     4861 gccgggtggt ggataccagc gagcttatcc gggtgtacgg tgagttaaga caggatgaga
     4921 ctcacgagcg ggacgagttg agactgtcgg gtgagactga aaaaatggcc tccgacgcgt
     4981 tgatcgcgga gataaaagca ctcagggagg aggtggccgg gctacggcag gaaatgcagt
     5041 ccatgagact gctggaacac aaaccggaga cagcagagac cccggccgga acaccgcgcc
     5101 ggtggtggcc gtggggaaaa cgctgacgcg ctgtgcttgt ccaaccggcg acggccacgc
     5161 aatgaattgc gtccccgtca ccggttatta gagaacaata tggggattct ggcgctggcc
     5221 gaccacggcc agaacccgga cttttctggt ctggtcatca acatcataga ggacatgctg
     5281 tcccatcaga ctgatccggc gaacgtcctc gccatagcgt gagtccactt catagagtcg
     5341 cgggaagtcg gccagtttgt ccgccgcagc gagaacccgg tcggtaacgt tctgcgcatc
     5401 ccacagggat gaattttccg cgatatagtc aaacagcgtc gttacatcct cgatggctcc
     5461 ctgcttccat tccacgtcat acggcatcgc gcagttcctg ataacggcca tgcaggcgct
     5521 ggcggagttg ttccgtggtc atacccggac gcgtgtcagc acgggcggcg gtcactttgg
     5581 cgcgaacctg atcttcatac tctgcctggg ccagttcttc gttagccagt cgctgcaaca
     5641 gcgcagcttt gtccgggcca ccttccggca gtatgcgggg caggctttca gccagttccc
     5701 gaagaaagtt ggcgttttcg tagtgtgtag tgttgctcat tgcggagcct cccgggttaa
     5761 tgcggcaata tgaaggaagt ttagcagggg gcggtctgaa agaatatggt cgtgtcagtc
     5821 aggctgatga agcagaaagt cggtaaaaaa gggcttacgg catccatttt acgtcaaaac
     5881 atatgccatg cagttgcgtg cataggcatg cattaggata aaatttatcg ggcgcgtttc
     5941 cggcagtttt ccgggggggt tgttgcctgt tttatcccgt agccgccgga aacgccctga
     6001 gcccgtctga gcggtgcgcg taatgacgcg ttatggtaaa tccacttact gtctgccttc
     6061 atagccatcg agataaacag ctgaaatggt gtcagccagt agccttgatt ttttcggtgt
     6121 gaggtctttg ctgcggtaat cggtacccgc aaattgtgtt gtgccttgga gtgagtttaa
     6181 aaattcctga ctgtgaaact gctgaatgcg ttgctgcaca tcactccgtg attttccggt
     6241 cgctttatcc tttgcgccca tatcccagaa ctgccggaaa aaaggcacca tcattcgtgt
     6301 tgtgtcagct ttctgggtgg aaaaattggg atcaaaaccg tcatctgaag catatacgta
     6361 atgccgggta tgctgttcca cgctgccctt tggagcacag gcagtgagag ttgctgtcgc
     6421 tgttatcatg gcgataagac acagtttgct gccggaacgt ctcatgagcg tctccttacc
     6481 tttaattctt ttttctcatt gagttatata tccgcaggct atcttatcat cagtttctga
     6541 ccgtgttcag tgaccgaaca acccgaacca gaccccccgt ttcttttcct gtcccggagg
     6601 gggctttctg gtatctcata taatgatttc agtaaaacat actgaggccg gagaacggcg
     6661 ccgtttgtgc tggtttttgt tgcatctgga ggggattgcg catggtgtga gtggctgttt
     6721 ttttgatcat tttcacaaaa acgcaaattt acagacttga catgaaaaac aaggcagcgt
     6781 aatttttgta ctgtatataa aaccagtggt tatatgtaca gtattttatt ttagtttatt
     6841 gttttaaaag tcaaagagga aaagtatatg agtggcggag acggacgagg tccgggtaat
     6901 tcaggtctgg gacacaatgg tggtcaggcc agtgggaatg tgaacggtac atcaggtaaa
     6961 ggtggccctt catcaggtgg tggtacggat ccaaacagcg ggccgggctg gggtacgacg
     7021 catacaccta acggggatat tcataactat aacccggggg agtttggtaa cggcgggagt
     7081 aaacccggtg gtaatggcgg taacagcggc aatcatagcg ggagctctgg tggcggacag
     7141 tcttcggcca ccgcgatggc cttcggtctg cctgccctgg ccactccggg ggctgaagga
     7201 ctggctttat ccgtttccgg cgatgcgttg tcggccgctg ttgctgatgt gctggctgcc
     7261 ctgaaaggtc cgtttaagtt tggtctgtgg gggattgcga tttacggagt gctgccttct
     7321 gagatagcaa aagatgatcc gaaaatgatg tcaaaaatcg tgacgtcatt gccggcagac
     7381 acggtaacgg agacgccggt cagctccctg ccgctggacc aggcgacggt cagcgtcact
     7441 aaacgtgtgg cggatattgt gaaggacgag cggcagcata ttgcggttgt caccggccgg
     7501 ccaatgagtg ttcctgtggt ggatgcgaaa ccgacaaaac gtccgggggt attcagtgtg
     7561 tcgattccgg gtctcccgtc tctgcaggtg agcgtaccta aaggtgttcc ggccgcgaaa
     7621 gccccgccaa aaggcattat tgctgaaaaa ggtgattcac gtccggctgg ttttacggcc
     7681 ggtggtaact cccgtgaggc cgttattcgt ttcccgaaag agagcggaca gaaaccggtc
     7741 tatgtgtcgg tgacggatgt tcttactccg gcacaggtaa aacagcgtct ggaggaagaa
     7801 aagcgtcgcc agcaggcatg ggacgctgct cacccggaag aggggctgaa aagagagtat
     7861 gataaagcga aagctgagct ggatgctgaa gataaaaata ttgcgacctt aaacagtcgc
     7921 attgcatcga cagagaaggc gatccccggt gcaagggctg ctgttcagga agccgataaa
     7981 aaggtaaaag aggcagaggc aaataaagat gattttgtga cttataaccc gcctcatgaa
     8041 tatggctccg ggtggcagga tcaggttcgc tatcttgata aggatattca gaatcagaat
     8101 gagaaattaa aagctgccca gacatcttta aacgaaatga atgaatcctt atccagggat
     8161 aaggctgcgc tttccggggc gatggagagc cggaaacaaa aggagaaaaa agcgaaggat
     8221 gcagaaaata aattaaatga ggaaaagaaa aaacctcgta agggaactaa agattacggc
     8281 catgattatt ttcctgatcc caagactgaa gatattaagg ggttgggaga gttgaaagag
     8341 ggtaaaccta aaacccctaa acaaggtggt ggtggtaagc gggcccgatg gtatggtgat
     8401 aaaaagcgta aaatttatga atgggattcc cagcacggtg agcttgaagg gtaccgcgcc
     8461 agtgatggcg aacacctcgg ggcattcgat ccaaaaacgg gtaagcaggt taaagggccg
     8521 gatccaaaac gaaacattaa aaaatatctt taagaggtaa atatggggct taaattacat
     8581 attcattggt ttgataagaa aaccgaagag tttaaaggtg gtgaatactc aaaagacttc
     8641 ggtgatgatg gttctgtcat tgaaagtctg gggatgcctt taaaggataa tattaataat
     8701 ggttggtttg atgttgaaaa atcatgggtt tcgatattac agccacactt taaaaatgta
     8761 atcgatatta gtaaatttga ttactttgta tcctttgttt accgggatgg taactggtaa
     8821 gcaaaagtta tcaaggatga tttgaaatac agacttccgg tgttcaggga tgaataccgg
     8881 ggtttttaaa tacagggaag gtatatgaaa aaggtaaagg ttgtttttct ttttatattg
     8941 attttttcag gctttcttct ggtcgcatgt caggcaaact atatccggga tgttcagggt
     9001 ggaacggtgg caccatcgtc ctcctctgaa ctgacgggga tcgcggttca gtagaaaaga
     9061 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat cttttgccct gtaaacgaaa
     9121 aaaccacctg gggaggtggt ttgatcgaag gttaagtcag ttggggaact gcttaaccgt
     9181 ggtaactggc tttcgcagag cacagcaacc aaatctgtcc ttccagtgta gccggacttt
     9241 ggcgcacact tcaagagcaa ccgcgtgttt agctaaacaa atcctctgcg aact
//
