LOCUS       NZ_CP046545             2571 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FHI_NMBU_10 plasmid pFHI_NMBU_10_06,
            complete sequence.
ACCESSION   NZ_CP046545
VERSION     NZ_CP046545.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13476489
            Assembly: GCF_016803915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Finton,M.D., Lindstedt,B.-A. and Brandal,L.T.
  TITLE     Escherichia coli strains with combined IPEC and ExPEC virulence
            factors isolated from human faecal samples
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Finton,M.D., Lindstedt,B.-A. and Brandal,L.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Faculty of Chemistry, Biotechnology and Food
            Science, Norwegian University of Life Sciences, Chr. Magnus
            Falsensv. 1, Aas N-1430, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-DEC-2019
            Assembly Method                   :: Unicycler v. 0.4.8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.21x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:11:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,248
            Genes (coding)                    :: 5,069
            CDSs (with protein)               :: 5,069
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 74 of 179
            Pseudo Genes (incomplete)         :: 112 of 179
            Pseudo Genes (internal stop)      :: 35 of 179
            Pseudo Genes (multiple problems)  :: 39 of 179
            Pseudo Genes (short protein)      :: 3 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046545.1.
            Bacteria and source DNA available from Norwegian Institute of
            Public Health, Oslo, Norway.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FHI_NMBU_10"
                     /serotype="O146:H21"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pFHI_NMBU_10_06"
                     /geo_loc_name="Norway:South-east"
                     /lat_lon="59.935 N 10.746 E"
                     /collection_date="2012-12"
                     /collected_by="Norwegian Institute of Public Health"
                     /genotype="ST829"
     gene            complement(1163..1375)
                     /locus_tag="GOE21_RS26585"
                     /old_locus_tag="GOE21_26580"
     CDS             complement(1163..1375)
                     /locus_tag="GOE21_RS26585"
                     /old_locus_tag="GOE21_26580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_128936982.1"
                     /translation="MRPLTENELKNVSGALYESVNTNCVLASGAMIVSAFGGPIGLIST
                     GLAAIGACATSSSSSSASASAGNGS"
                     /name="hypothetical protein"
     gene            complement(1395..1712)
                     /locus_tag="GOE21_RS26590"
                     /old_locus_tag="GOE21_26585"
     CDS             complement(1395..1712)
                     /locus_tag="GOE21_RS26590"
                     /old_locus_tag="GOE21_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V family bacteriocin"
                     /protein_id="WP_021574241.1"
                     /translation="MREITLNEMNNVSGAGDVNWVDVGKTVATNGAGVVGGAFGAGLCG
                     PVCAGVFAVGSSAAVGALYDAAGNSKPTKQKPEGLPPEAWNYAEGRMCNWSPNNLNDVC
                     L"
                     /name="colicin V family bacteriocin"
     gene            complement(2051..2488)
                     /locus_tag="GOE21_RS26595"
                     /old_locus_tag="GOE21_26590"
     CDS             complement(2051..2488)
                     /locus_tag="GOE21_RS26595"
                     /old_locus_tag="GOE21_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001557684.1"
                     /translation="MNTQNLIAEHREKHDRDNLGFTQVYPLGWKRLMALAKGNQGAFGL
                     YTFLAQHIDGACGAVVADQAFLADQFDVTTRTVRNWISYLEEQKAIVKIPVAGRVCAYA
                     LNPHEVWKGYDNNKPTAAFLTKTLVSKDGEIKRRIMAMFSN"
                     /name="hypothetical protein"
     Typing          complement(317..401)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.837"
                     /coverage="100.0"
                     /evalue="1.41e-35"
ORIGIN
        1 agaagtgata ggaaacacat gtgtttccta tcatgcggaa acacatgtgt ttcctatcag
       61 gctacatttc tataaataaa aacaataact tacatgttcc gctaattgtt atcttaagag
      121 cactatgttt cctaatgcac gatcgtttgg ttttggtttt tttggggttt cggggcgaag
      181 ccctgaacca gtcacacagc gatagcggag tgtatactgg cttactcctc tgcaagcctc
      241 gtcagccgtg atttttgtcc gatttcgtgc tctccacgtt caacagctcc atcatgtttt
      301 tgccgtccgt tcaccgctaa aacggctcag aaatcgggac aggatgtggg aatgtcggac
      361 cgcgcacgcg cactcacaca ttccccacct ggtcagggct tcaccccgac accccgcatc
      421 agtgcgtaag tgctttatgt ggcttccggt tctcttccgc ttcctcgccc actcgctcac
      481 tacgttcggt cgctggggct gcggcgagcg gtgtcacctc tcgcatgttg tcagcgattc
      541 tgttgctgtt ctgttcgaaa atgcgttctg taagccatcc agagcaccag aacgcgattt
      601 tctggtgtct gtaatggttg ggtacatatt ggcccttaaa acgcactcag aagctcccag
      661 acgcacgaaa cggggtttaa atgcccgcca gggcatggaa ggcgcgaaaa acgcttacag
      721 agcgtttcag gaggtcaggc gcgccgatac ccctaaccag gggctttcag gtcgccagga
      781 accgcgccag ccccctgcaa agcccatttg cacggaacag acagccactc ggctggtagg
      841 tggtcgtaga ccgtcgatgt tgcagcgatg ggggtctggg gttctcccca gacggtttcc
      901 ggttgcgctc aaggcgctgc cggaaccgtg aaagttattt agctgactga attcgtctta
      961 ttttcaaaag aagaattctc tcccatataa ttaatattcc tgtaaagaat attaaccagt
     1021 ctttattagg tagctgatat aaaatgaaca tcaacattac agaaataata gttgtaacta
     1081 tgatattcac gtattctttc gagaaaaata tagatttaag ttctaactta atcaattcgt
     1141 cgaacatgaa tactcctaat agttaagatc cgttccctgc agaagcacta gccgaagatg
     1201 acgatgatga agtggcgcaa gcacctatgg ctgctaatcc agtagatatt aaacctatag
     1261 gtccaccgaa agcagatact atcattgctc cagaagctaa aacacagtta gtattaacgc
     1321 tttcatacaa agcaccagat acatttttca attcattttc tgttaatggt ctcattaaat
     1381 aattcctttt catattataa acaaacatca ttaagattat ttggactcca attacacatt
     1441 ctaccttcag catagttcca tgcttctggt ggcagccctt ctggtttttg tttcgttggt
     1501 ttggaatttc ctgctgcatc atacaacgca ccaacagcag cagaagatcc gacagcgaac
     1561 acaccggcac aaacagggcc gcatagcccc gctccgaacg caccgccgac cacaccagct
     1621 ccgtttgtag ctacagtttt tccgacatca acccaattga catcaccagc accagacaca
     1681 ttattcattt catttagcgt tatttctctc atacgtcact tatctcctat taacaaaaat
     1741 agatatcata aatgcaatag cgataccgct aactatagac tgaacaaggt tgctagcatt
     1801 tagctgtttc gcaacaagac taataataat gcttatgggt aatataatga agaatacttg
     1861 ccacgttttc atttatattc ctatgcttaa tttatagtaa cgttcacaat acgatcgttc
     1921 atttcatatt aaaagttgta tatataaaaa tatgatttgg gtaacaattt tattaccatt
     1981 ggataaatgc aggcagacaa tcatggtcaa aacgttaaca cgtagccctg attgtctgcc
     2041 tgccgctggc tcaatttgaa aacatggcca taatccggcg cttaatttcc ccatctttgc
     2101 tgactaaggt ctttgttaag aatgcagctg ttggcttgtt attgtcatag cctttccaca
     2161 cctcgtgtgg gttaagagca taggcgcaaa ctcttcccgc aacgggaatt ttgactatgg
     2221 ctttctgctc ttctaggtac gagatccaat tgcgtacagt tcgagtggtc acatcaaact
     2281 gatcagccag aaaagcctga tccgctacaa cagcaccgca cgcaccatcg atatgctgtg
     2341 ccaaaaaagt atacaagcca aatgcgccct gatttccttt ggcaagagcc atcagccgct
     2401 tccatcctag tggatacact tgagtgaatc cgaggttatc tctgtcatgt ttttctcgat
     2461 gttctgcgat tagattctgg gtattcatag cgattcctat caaccggaaa ttatcattcc
     2521 tatagtagga aatttatttt ccacatcaac gcttatttca ttcctacttt c
//
