LOCUS       NZ_CP046544             3356 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FHI_NMBU_10 plasmid pFHI_NMBU_10_05,
            complete sequence.
ACCESSION   NZ_CP046544
VERSION     NZ_CP046544.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13476489
            Assembly: GCF_016803915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Finton,M.D., Lindstedt,B.-A. and Brandal,L.T.
  TITLE     Escherichia coli strains with combined IPEC and ExPEC virulence
            factors isolated from human faecal samples
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Finton,M.D., Lindstedt,B.-A. and Brandal,L.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Faculty of Chemistry, Biotechnology and Food
            Science, Norwegian University of Life Sciences, Chr. Magnus
            Falsensv. 1, Aas N-1430, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-DEC-2019
            Assembly Method                   :: Unicycler v. 0.4.8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.21x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:11:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,248
            Genes (coding)                    :: 5,069
            CDSs (with protein)               :: 5,069
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 74 of 179
            Pseudo Genes (incomplete)         :: 112 of 179
            Pseudo Genes (internal stop)      :: 35 of 179
            Pseudo Genes (multiple problems)  :: 39 of 179
            Pseudo Genes (short protein)      :: 3 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046544.1.
            Bacteria and source DNA available from Norwegian Institute of
            Public Health, Oslo, Norway.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FHI_NMBU_10"
                     /serotype="O146:H21"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pFHI_NMBU_10_05"
                     /geo_loc_name="Norway:South-east"
                     /lat_lon="59.935 N 10.746 E"
                     /collection_date="2012-12"
                     /collected_by="Norwegian Institute of Public Health"
                     /genotype="ST829"
     gene            254..481
                     /locus_tag="GOE21_RS26570"
                     /old_locus_tag="GOE21_26565"
     CDS             254..481
                     /locus_tag="GOE21_RS26570"
                     /old_locus_tag="GOE21_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042968139.1"
                     /translation="MSIDVIKQIQSIQDKGIIIYSKYRANEFNRDDVYRESYSLIIEFN
                     HLIAEHAVHDEKLINHTVCILNELRSIAIN"
                     /name="hypothetical protein"
     gene            complement(1148..2146)
                     /locus_tag="GOE21_RS26575"
                     /old_locus_tag="GOE21_26570"
     CDS             complement(1148..2146)
                     /locus_tag="GOE21_RS26575"
                     /old_locus_tag="GOE21_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_086257818.1"
                     /translation="MKNPELKNLTDYSPEDKPWDERRSFSDDVGGIYLRAAGFEHYGER
                     VGACSGILRFGWSTNTETGETRLRLRDARFCRVRHCPICQWRRSMMWQARFYQSLPKIV
                     TEHPGARWLFLTLTVRNCSIDALGETLTAMNAGFQRLKDRKEFRHVLGWVRTTEVTRGK
                     DGSAHPHFHTLLMVPPSMLSGREYVKHARWVELWCDCLRVAYDPNVDIRVVKSRKPKDD
                     ESLPCVVAEQLRGAVAETLKYSTKPADMLADPEWFLELTRQVHKRRFVATGGALKNVLQ
                     LERETNEDLIMADGMAEGTDDGSRLAFSWVPVVKKYQRDRAKDKRRLSSDD"
                     /name="protein rep"
     gene            2986..3180
                     /locus_tag="GOE21_RS26580"
                     /old_locus_tag="GOE21_26575"
     CDS             2986..3180
                     /locus_tag="GOE21_RS26580"
                     /old_locus_tag="GOE21_26575"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="WP_123129595.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIEAWKKWNDALHYAKG
                     ENIGWCIARAEYCKKTGRF"
                     /name="ANR family transcriptional regulator"
     Typing          complement(745..822)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.5"
                     /coverage="87.0"
                     /evalue="5.64e-30"
     Typing          complement(2726..2808)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.706"
                     /coverage="93.0"
                     /evalue="7.11e-14"
     Typing          1240..1431
                     /name="Col(MP18)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.964"
                     /coverage="100.0"
                     /evalue="6.14e-93"
ORIGIN
        1 aaatcagtaa atatctaact agtgtccatc taaggtctat ctaacgtcca tctaaaagct
       61 aactaacggt tcaccaacgg atcgcatgta gtagacgcca cataaaaaat cctgaaatgg
      121 aggaatcgtc ataaagagat tcctgttttt agcgttctga tgcaggatag ggcaaacgcc
      181 gcaaaatgac gtccttgata tattttgtga tgtttgtttc gggcatctgt tatctgtttt
      241 attgggtgtt tttatgagta ttgatgtgat taaacaaatt cagagcatac aggacaaagg
      301 aattattatt tattcaaaat acagagcaaa tgaatttaac cgggatgatg tatacaggga
      361 gagttacagc ctgattattg agtttaatca tcttattgct gaacatgccg ttcatgatga
      421 aaaactgatt aatcatactg tctgtattct gaatgaatta cgaagcatag cgataaatta
      481 aaaaatcccc ctggattaca gggggaagcc atcagagttt gagtaatgag aaatattatt
      541 ttggagagag caccacaaat gcgctctttt ccaatctgcc gtaaccatca gcaaaagaca
      601 atcaggacag gcgaaaaaat ccccctatat ggcaaggggg tgtcaacgag gacatggcaa
      661 ttgtgagttt tgtgtgtttt atgtgttgca gtttgcaacg tcagaaattt gccgcagtta
      721 tgcgcgaaaa acaatcctga tttcgggaca ggatgtgtaa tcgtataacc gcgcacgcac
      781 ggctatacga ttaccacctg gtcagggctt tgcccgacac cctcgacacc gttcccgcaa
      841 tacgatagaa catcagggcg cgtttccggg taaacgcatg ttaatgaccg ggaattcatt
      901 gattagggac cggagacgcg ctacaaaact cctgcgcgtt tctggcatca actgaccatg
      961 gggatggttt ggcaatagca gcaagcgcca gaaacgcgct caaaacgtct ctgacggcct
     1021 gaatcaacca aaacatataa aaccattgcc tgatgcaaaa aatcgcgtta gaaagcgtct
     1081 gagtgcgttt ctggtgagca aaaagctgtc agccgtggat tgccattgca gagacccaga
     1141 aacgcgctca atcatcgctg cttagccgac gtttatcttt cgcccgatcg cgctggtatt
     1201 ttttgacaac cggcacccac gaaaacgcca gacgggaacc gtcatctgta ccttctgcca
     1261 tgccatccgc catgattaaa tcctcgttcg tttcccgctc cagttgcaga acgttcttaa
     1321 gcgctccccc cgtggcaaca aaacgacgct tgtgtacctg ccgtgttaat tcgaggaacc
     1381 attcaggatc agccagcata tcagcaggct ttgtggaata tttcagagtt tcagccaccg
     1441 cgccgcgcag ctgttccgca acaacacaag gtaaagattc atcgtctttt ggtttccgtg
     1501 acttcaccac tctgatatcc acattcgggt catacgccac ccgcagacaa tcacaccaca
     1561 gttccaccca acgggcatgt ttcacatact cacggccgga caacatcgag gggggaacca
     1621 tcagcagagt gtgaaaatgg ggatgtgcgc taccatcctt gccgcgcgtt acttccgtgg
     1681 tacgtaccca tcccagcaca tgacgaaatt ccttgcgatc tttcagccgc tgaaaaccag
     1741 cattcatcgc ggtcagcgtc tcaccaagcg cgtcaatgga acagttgcgc actgtcagcg
     1801 tgagaaacaa ccagcgcgcg ccgggatgtt ccgtcacaat ctttggcagg gactgataga
     1861 aacgggcttg ccacatcatg gagcgcctcc actggcagat cggacagtgc cggacacgac
     1921 agaaccgcgc atcacgcaac cgcaggcgcg tttctccggt ctctgtgttt gttgaccacc
     1981 caaaccgcaa aataccacta caagccccga cacgttcgcc gtagtgctca aatccggcag
     2041 cacgcagata aataccgccc acatcgtcgg aaaagctccg gcgctcatcc cacggtttat
     2101 cctccgggga ataatccgtc agatttttaa gttcaggatt tttcatgtct gccgttccct
     2161 agtgagcgac agacgaaatt acgtgttatc attcctgtat cacctgtgtt gtcgtgtcac
     2221 caattcgaat tcctacaggt ggtcacaccc ccgaacgatg ccagtcgttg cgggggtcgc
     2281 ttttaatgcg accacttccg acaaaatact ctcaaaacca tttccgagca aatctgcgct
     2341 cagcccagat gcgacaaggg ctagagctaa attacaatcc cagagatatc ccttaagtat
     2401 aaatacaagg atcgcgcgca cgatcaccca cccaaaatgc gcgttttttg agcgtttccg
     2461 agcacaaaaa aacaacacac tgaaaccact ctcccgacaa tactccagca ggtttctcac
     2521 cgactttctg aacaaaaaaa ccagctttgc aggtggttgg tcccactgcg tgacaccaac
     2581 caccatcacc agtgtttttt ctcgtgccgt cgtcaatcac aaaatcggaa cccacgccgg
     2641 aggcgcggga ccccacgatt ttctgtttga cgagtcagaa aacggcgaac gctgacagat
     2701 ttcaatctgg ctgcgtgaga cgttggcggg gtgtcggggc gcagccctga ccaggcggca
     2761 tttgtctgat tgcgtgtgcg cggtccgaca aatacacatc ctgtcccgtt tttcgcacgg
     2821 tttttattga ttaccggaca gatgaatccg gattgccatg ttgcgaaaat cacgttaaaa
     2881 cgattacgag cgtgcagttg ggctgatata tcaacagtca tgccaatatg tattaacccg
     2941 acaggccgct cttttttaat tcaaataaaa atataaatta cgagtatgaa tcaggacaaa
     3001 gcaatttatt tagaaaataa atatttttca cccgcaaacg aagctgtcag actggagcgt
     3061 gaaggagatt acattgaagc atggaaaaaa tggaatgatg cgcttcatta tgccaaagga
     3121 gaaaatatag gttggtgtat agccagagct gaatattgta aaaagacagg ccggttttaa
     3181 ttctggcggg gtttcaaagg ggcgtcccct ttggcacgtc gacgtatcaa aatgcgccag
     3241 cattttgtat acagtcggtc acgtgcctcc aggattgccc cataatattt cctatctaca
     3301 taatgtctaa ctaagatcca tctaatatcc atctaagatc catttaaggt ccatct
//
