LOCUS       NZ_CP046543             5631 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FHI_NMBU_10 plasmid pFHI_NMBU_10_04,
            complete sequence.
ACCESSION   NZ_CP046543
VERSION     NZ_CP046543.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13476489
            Assembly: GCF_016803915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Finton,M.D., Lindstedt,B.-A. and Brandal,L.T.
  TITLE     Escherichia coli strains with combined IPEC and ExPEC virulence
            factors isolated from human faecal samples
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Finton,M.D., Lindstedt,B.-A. and Brandal,L.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Faculty of Chemistry, Biotechnology and Food
            Science, Norwegian University of Life Sciences, Chr. Magnus
            Falsensv. 1, Aas N-1430, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-DEC-2019
            Assembly Method                   :: Unicycler v. 0.4.8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.21x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:11:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,248
            Genes (coding)                    :: 5,069
            CDSs (with protein)               :: 5,069
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 74 of 179
            Pseudo Genes (incomplete)         :: 112 of 179
            Pseudo Genes (internal stop)      :: 35 of 179
            Pseudo Genes (multiple problems)  :: 39 of 179
            Pseudo Genes (short protein)      :: 3 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046543.1.
            Bacteria and source DNA available from Norwegian Institute of
            Public Health, Oslo, Norway.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FHI_NMBU_10"
                     /serotype="O146:H21"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pFHI_NMBU_10_04"
                     /geo_loc_name="Norway:South-east"
                     /lat_lon="59.935 N 10.746 E"
                     /collection_date="2012-12"
                     /collected_by="Norwegian Institute of Public Health"
                     /genotype="ST829"
     gene            join(5337..5631,1..236)
                     /locus_tag="GOE21_RS26535"
                     /old_locus_tag="GOE21_26530"
     CDS             join(5337..5631,1..236)
                     /locus_tag="GOE21_RS26535"
                     /old_locus_tag="GOE21_26530"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673158.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVNRELTSNE
                     SLIRNAINDHTTALKELLERYQKTTVDTMDAHWKTVLKMSVKRWLWLIIVSVLMFATTG
                     SLLWYQGMKINANMNILREQKESLEKLNAKTWGVRYHEDSNGRFLVLPKGMKAETNWTK
                     DNGKLNAVRLVQE"
                     /name="MbeB family mobilization protein"
     gene            243..476
                     /locus_tag="GOE21_RS26540"
                     /old_locus_tag="GOE21_26535"
     CDS             243..476
                     /locus_tag="GOE21_RS26540"
                     /old_locus_tag="GOE21_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137437.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWENAFVELQKMFSLTQRDNA
                     MLNGRVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            957..2009
                     /locus_tag="GOE21_RS26545"
                     /old_locus_tag="GOE21_26540"
     CDS             957..2009
                     /locus_tag="GOE21_RS26545"
                     /old_locus_tag="GOE21_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_086257810.1"
                     /translation="MNLQQRINKLPQLSSSFSFGKDIDNIHSFIFNETSKDKIEDLLRK
                     WVSGNQPCVFGKLASKKIKGLDFHLSIVNSPQLYNDDGHLFDFLRNERVRFKERARRGE
                     VSAHLIYFIHPQLAFARPSEELVDIQKYICSLHMPECYPIKEDVIYTESVPFQDKDGLK
                     IYKAGVNVFYSSAHRTRNHDRRIPGGILISVNAPGHFMRLAIENGFYKDQEQALADIRN
                     MTIQSVGKGGYSHPEGISTTWHSESNLDRFGCPVHTGNSSYYSGFYHTDVLIPGELTKD
                     ERLLHEIENSDPMIFNWNVLFYVSLEEFPIDDPYYGEFIGIPVDDASMFFNSFQPRKFE
                     NKPLYEKEDD"
                     /name="hypothetical protein"
     gene            2011..2877
                     /locus_tag="GOE21_RS26550"
                     /old_locus_tag="GOE21_26545"
     CDS             2011..2877
                     /locus_tag="GOE21_RS26550"
                     /old_locus_tag="GOE21_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335763.1"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_001335763.1"
                     /translation="MIKGMLFAFLAAFSWGAAIVMSKKGVEELDAGGVFFWQICSAVIL
                     SWIVLFIKRKKLPLNSKAILAYATGVFEPFLAYTFTLYGLYFISAGSASIIFSLESVFI
                     LVLSVLICSVKINSPQYFILFLIGAIVGSILVALPDISNGSNNIVGYCLVVAGVLSAAF
                     YVVISSRLIKSFEPITLLSGQLTFSLILSSLFIFISKSSIYLPLESVIIVFSSGILQYF
                     LAFCFYLNSLRWLPVHLAGIMLYFIPLVALFLSWLFLDEQFTFLQGVGFIVTVLSVYFL
                     NRKYSEE"
                     /name="DMT family transporter"
     gene            complement(4080..4271)
                     /locus_tag="GOE21_RS26555"
                     /old_locus_tag="GOE21_26550"
     CDS             complement(4080..4271)
                     /locus_tag="GOE21_RS26555"
                     /old_locus_tag="GOE21_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165975.1"
                     /translation="MTKQEKTALNMARFIRGQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENR"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4303..4659
                     /locus_tag="GOE21_RS26560"
                     /old_locus_tag="GOE21_26555"
     CDS             4303..4659
                     /locus_tag="GOE21_RS26560"
                     /old_locus_tag="GOE21_26555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000538661.1"
                     /translation="MGVMIPMKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGE
                     PVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRR
                     LRLAVREQGARDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          3905..3986
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          3261..3448
                     /name="rep_cluster_2401"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="4.63e-94"
     Typing          3..580
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="7.56e-98"
     Typing          4650..5629
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgtttgccac gaccgggagt ctgctgtggt accagggcat gaagataaac gcgaatatga
       61 atattctccg cgagcagaaa gagagtctgg agaagctgaa cgcgaagaca tggggcgtgc
      121 gttatcacga ggacagcaac ggacgttttc tggtgttgcc gaaggggatg aaagccgaaa
      181 cgaactggac gaaggacaac ggcaaactga acgcagtgag actggtgcag gagtgacaga
      241 gcatgacgga gctggaaaga catttgctga acgcattaga gcaactgcaa caggactata
      301 tgcagcggct gaacgaatgg gagaacgcct tcgtggaatt gcagaaaatg ttttcgctta
      361 cgcaacggga caacgcgatg ctgaacgggc gagtcatgca gttgagtcag caggtgcagc
      421 acttgagcga gcggacagaa cgcttgagcc agttatacag cgagaacagg agataaggga
      481 tgaacggctg atgcaggaac gcgaacatga gttatctctg gagcgggagc gtcagccgga
      541 aatccaggag cggacactgg atggcccttc gctgggatgg tgaaaccatg aaaaatgacg
      601 tgttcagtgg attaaggcgg gctaatgtgg cctgtagcat ctggttgcat aggtattcat
      661 gcggttaaaa tttatcaggt gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt
      721 acctgtctgc tgccgtgatc gcaccgaacg cgttttggcg gtgcgtacaa tgcgacgtta
      781 tggtaaatgg agcggatttt caggaaggag tgatactaac aactcgaaca ctgtgcaaat
      841 aatgattttt ttaaaatatc ggtaacgata ataaattatt tcgttttacg ccggtaataa
      901 atataatgta gtttcagtat ataaaaaaat atatgaggtg tcttgtggga gctttcatga
      961 atcttcaaca acgtataaat aaactaccac aattatcatc cagtttttca tttggaaaag
     1021 atattgataa tattcattca tttatcttta acgaaacttc taaagataaa attgaagatt
     1081 tactaagaaa atgggtgtca ggcaatcagc cctgtgtatt tggtaaattg gcaagcaaga
     1141 aaattaaagg gcttgatttc catttatcta ttgtaaatag cccccaacta tataatgatg
     1201 atggtcattt gtttgatttt ttgcgtaatg aaagagttcg ttttaaagaa cgcgcaagaa
     1261 gaggagaggt cagtgcgcat ctcatttatt ttattcatcc acaacttgcc tttgctcgtc
     1321 catcagaaga gttagtggac atacaaaaat atatttgttc gctgcatatg ccagagtgct
     1381 atccaataaa agaggatgtt atttatactg aaagcgtacc atttcaggat aaagatgggc
     1441 taaaaatata taaggctggt gttaatgttt tttactcttc tgctcataga acaagaaatc
     1501 atgatagaag aataccaggg ggaatactta tttctgtaaa tgccccgggg cattttatga
     1561 ggctggcgat agaaaatggt ttctataaag atcaagaaca agcgttagcg gatattcgta
     1621 acatgactat tcagtctgtt ggtaagggtg ggtattcaca tccggaaggg atttctacaa
     1681 catggcattc agaatctaat ttggatcgtt ttggatgccc agtgcacacc ggtaatagtt
     1741 cttattacag cggtttttat catactgatg tattaattcc aggagaatta actaaggatg
     1801 aaagattgct ccatgaaata gaaaatagtg atccaatgat cttcaactgg aatgtattgt
     1861 tttatgtttc attagaagag tttcctatag atgatcctta ttatggagag tttattggta
     1921 ttcctgtaga tgatgcatct atgtttttta actcctttca accaaggaaa tttgaaaaca
     1981 aaccccttta tgagaaagag gatgattaat atgattaaag gaatgctttt tgctttttta
     2041 gcagcgttct catggggggc agctatcgta atgagcaaaa aaggagttga agaactagat
     2101 gccggagggg tgtttttctg gcagatttgt agtgcagtaa tactatcttg gatagttctt
     2161 tttataaaaa gaaaaaaact cccattaaac tcaaaagcga tacttgctta tgcgacaggc
     2221 gtttttgaac cattcttagc gtatactttt acactctacg gactttattt tatttcagca
     2281 ggaagcgcat caattatttt ttcacttgaa tcagtgttta tattggtgtt gtctgttctt
     2341 atttgctcag ttaaaattaa ttccccgcaa tattttatat tgttccttat aggtgcaatt
     2401 gttggcagta ttttggtagc cttgccagac atcagtaatg gatcgaataa tattgttgga
     2461 tattgtcttg ttgttgccgg tgtgttatcg gcagcatttt atgttgttat atcgtctcgc
     2521 cttattaaat cgtttgagcc tatcacatta ttgagcggac agttgacatt ctcccttata
     2581 ctgtcatcgc tctttatttt tatatctaaa tcatcaattt atcttccgtt ggaatccgtg
     2641 attattgttt tttcatctgg aatattacaa tattttttag ctttttgttt ttacttgaac
     2701 tccttgcgtt ggctcccagt tcatttggca gggataatgt tgtactttat tccgctagtt
     2761 gcattatttt tgtcatggtt gtttttggat gaacaattca cattcttaca gggggtagga
     2821 tttattgtaa ctgtgctaag tgtatatttt cttaacagaa aatactcaga ggagtaacat
     2881 tctgttcagg attgcccccc ggcctccatc gataccgtgc caaatgcacg taaaaaacgg
     2941 gagcgtttct ggtgtcggaa tgtaaccaag ggggtcagag gcgcgctaaa acgctctcag
     3001 gggagtcaaa tagagacgtt tgtaacaaaa agatcaaagg atcctcttga gatccttttt
     3061 ttctgcgcgt aatctgctgc ctgtaaacga aaaaaccgcc ttgcagggcg gtttgatcga
     3121 aggttaagtc agcttgcaga ctgcttaacc aggtaactgt cttatgcgag acgagtcacc
     3181 aaaattgttc ttctagtgta gccgcactcc ggcagccact tcaagtactc tctaaatatc
     3241 tcgcacatga cttttaccaa tggctgctgc cagtggcgct ttgtggtgtc ttttccgggt
     3301 tggacccaag agaacagtta ccggagaagg cgcagcagtc gggctgaacg gggggttcct
     3361 gcatacagcc cagcccggag cgaactgcct aagtcgcccc gagataccag aagacgtgag
     3421 ctatgagaaa gcgccacgtg tcccgtaagg gagaaaggcg gaaaaggtat ccggtaatcg
     3481 atatggcggg aacgtggggg cgcaggaggg agcgattcac cggaaacggt gatgatcttt
     3541 aagccacgtc gggtttcgcc accgctggcg gatttcatgg ttgagccgca aggctcccac
     3601 agatgcaccg aaaaagtgtc tgttttgtga agtccgtcag gggggcggag cctatggaaa
     3661 aagcgcctcc ggcgcggcct ttcccggggt ttcgtttttt gcgcacatgt tctttcctgg
     3721 tttatccccg ggttctgtgg ataacagggt tagcgttttt gagaaaggag agtccgctcg
     3781 ccgcagccga acgaccgagc gtagcgagtc agtgagcgag gaagcggaag agcgcctgaa
     3841 ctggcatttt ctccttacgc atctgtgcgg catttcacac cggacatggc gcacttttca
     3901 tacaatccgc actgatgccg aatggttaag ccagtataca ctccgctatc gctacgtgac
     3961 tgggtcaggg cttcgccccg acaccccaaa ggtcaacggc ctgacgcgcc ctggcgggct
     4021 tgtctgctcc cggcatccgc tcacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4081 cagcggtttt caccgtcatc cccgaagcgt gcgaggcaac tgcggtaaag ctcatcggcg
     4141 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagttc gttgagcttc
     4201 tccagcagcg ttaatgtctg gcctctgatg aagcgggcca tgttaagggc ggttttttcc
     4261 tgtttggtca ctgatgcctc cgtgtaaggg ggggttctgt tcatgggggt aatgataccg
     4321 atgaaacgcg agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
     4381 gaacgctgtg agggtaaaca actggcggta tggatgcggc gggtctgcct gggtgagccg
     4441 gttgcccgtt ccgggaagct gccgacactg gcaccgccgt tactgcgtca gctggccgcc
     4501 atcgggaata accttaacca gacagcgagg aaggtgaaca gcgggcagtg gtcttccggt
     4561 gaccgggttc aggtggtggc cgcactgatg gccatcgggg atgagctgcg ccggctgcgt
     4621 ctggcggtca gggaacaggg ggcgcgggat gatagttaag tttcatgcca ggggaaaagg
     4681 tggtggcagt ggtccggttg attacctgct gggaagggag cgtaaccggg aaggggcaac
     4741 ggtgcttcag gggaatccgg aagaggtccg ggaactcatc gatgccacgc catttgcgaa
     4801 gaaatacacg tcaggtgttc tgtcgttcgc ggagaaggag ctgccgcctg gagggcgtga
     4861 aaaagtgatg gcgagctttg agcgtgtact gatgcccggt ctcgagaaaa atcagtacag
     4921 catcctgtgg gtggagcacc aggacaaggg acggcttgag ctgaattttg tcattccgaa
     4981 catggagcta cagaccggaa aacgcctcca gccgtactac gaccgggcag acaggccgag
     5041 aattgatgcc tggcagacgc tggtgaatca ccattacggt ctgcatgacc ccaacgcccc
     5101 ggagaaccgc cggacgctga cccttcctga taacctgccg gaaacgaaac aggcgcttgc
     5161 tgagggcgtc acacgcggga tagacgcgct ttaccatgcc ggagagataa aaggccgtca
     5221 ggatgtgatt caggcgctca cagaggcggg gcttgaagtg gtcagggtga cgcgaagcag
     5281 tatcagcatt gcagacccga acggcgggag gaatatcaga ctgaaaggag cgttttatga
     5341 gcaatctttt acagacgggc gcggagttcg agaaaaagct gaaagagaga gccgaatcta
     5401 cagagaaaat gctgaacgac gagttcagga agctcgaaga atctgtaaac agggaactga
     5461 cctcaaacga atctttaatc aggaacgcta tcaacgacca tacaacagcc ctgaaagaac
     5521 tgctggagag gtatcagaaa acgaccgtcg atacgatgga cgcgcactgg aaaaccgtac
     5581 tgaaaatgag cgtgaaacgc tggctgtggc tgattatcgt ctcagtactg a
//
