LOCUS       NZ_CP046541             7047 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FHI_NMBU_10 plasmid pFHI_NMBU_10_02,
            complete sequence.
ACCESSION   NZ_CP046541
VERSION     NZ_CP046541.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13476489
            Assembly: GCF_016803915.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Finton,M.D., Lindstedt,B.-A. and Brandal,L.T.
  TITLE     Escherichia coli strains with combined IPEC and ExPEC virulence
            factors isolated from human faecal samples
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Finton,M.D., Lindstedt,B.-A. and Brandal,L.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Faculty of Chemistry, Biotechnology and Food
            Science, Norwegian University of Life Sciences, Chr. Magnus
            Falsensv. 1, Aas N-1430, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-DEC-2019
            Assembly Method                   :: Unicycler v. 0.4.8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 62.21x
            Sequencing Technology :: Oxford Nanopore MiniION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:11:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,377
            CDSs (total)                      :: 5,248
            Genes (coding)                    :: 5,069
            CDSs (with protein)               :: 5,069
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 97
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 74 of 179
            Pseudo Genes (incomplete)         :: 112 of 179
            Pseudo Genes (internal stop)      :: 35 of 179
            Pseudo Genes (multiple problems)  :: 39 of 179
            Pseudo Genes (short protein)      :: 3 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046541.1.
            Bacteria and source DNA available from Norwegian Institute of
            Public Health, Oslo, Norway.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FHI_NMBU_10"
                     /serotype="O146:H21"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pFHI_NMBU_10_02"
                     /geo_loc_name="Norway:South-east"
                     /lat_lon="59.935 N 10.746 E"
                     /collection_date="2012-12"
                     /collected_by="Norwegian Institute of Public Health"
                     /genotype="ST829"
     gene            complement(478..900)
                     /locus_tag="GOE21_RS26445"
                     /old_locus_tag="GOE21_26445"
     CDS             complement(478..900)
                     /locus_tag="GOE21_RS26445"
                     /old_locus_tag="GOE21_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_086257803.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGVKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTT
                     FAGRKYTNRDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1022..1300
                     /locus_tag="GOE21_RS26450"
                     /old_locus_tag="GOE21_26450"
     CDS             1022..1300
                     /locus_tag="GOE21_RS26450"
                     /old_locus_tag="GOE21_26450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000710675.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(1292..1582)
                     /locus_tag="GOE21_RS26455"
                     /old_locus_tag="GOE21_26455"
     CDS             complement(1292..1582)
                     /locus_tag="GOE21_RS26455"
                     /old_locus_tag="GOE21_26455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032330705.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEYDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            1823..3469
                     /gene="cka"
                     /locus_tag="GOE21_RS26460"
     CDS             1823..3469
                     /gene="cka"
                     /locus_tag="GOE21_RS26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_172398166.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEAKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVTYDEWAKHLEKIS
                     KALKVTGYLSFGNDVWDGTRKGLETGDWRPLFVTLEKSAVDFGVAKIVALMFSFIAGAP
                     LGFWGIAIITGIVSSYIGDDELNKLNELLGI"
                     /name="cka"
     gene            complement(3466..3756)
                     /locus_tag="GOE21_RS26465"
     CDS             complement(3466..3756)
                     /locus_tag="GOE21_RS26465"
                     /inference="COORDINATES: protein motif:HMM:NF037571.2"
                     /note="Members of this family include Cki (colicin K
                     immunity protein) for the pore-forming toxin Cka (colicin K
                     activity protein), as well as immunity proteins from other
                     related colicin systems.; Members of this family include
                     Cki (colicin K immunity protein) for the pore-forming toxin
                     Cka (colicin K activity protein), as well as immunity
                     proteins from other related colicin systems; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cki family colicin immunity protein"
                     /protein_id="WP_071829475.1"
                     /translation="MYLKYYLHNLPESLLPWLLYLTLNYNNDISLAFIFFASIHVLLYP
                     YSKLTIFSFIQKTTNIKKEPWYSYNLFYLLYLAMAIPVGLPSFIYYTLKRY"
                     /name="Cki family colicin immunity protein"
     gene            3781..3912
                     /locus_tag="GOE21_RS26470"
                     /old_locus_tag="GOE21_26465"
     CDS             3781..3912
                     /locus_tag="GOE21_RS26470"
                     /old_locus_tag="GOE21_26465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071699850.1"
                     /translation="MKIVLFLITILSLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4123..4267)
                     /locus_tag="GOE21_RS27040"
                     /pseudo=""
     CDS             complement(4123..4267)
                     /locus_tag="GOE21_RS27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(4981..5172)
                     /locus_tag="GOE21_RS26480"
                     /old_locus_tag="GOE21_26475"
     CDS             complement(4981..5172)
                     /locus_tag="GOE21_RS26480"
                     /old_locus_tag="GOE21_26475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5240..5563
                     /locus_tag="GOE21_RS26485"
                     /old_locus_tag="GOE21_26480"
     CDS             5240..5563
                     /locus_tag="GOE21_RS26485"
                     /old_locus_tag="GOE21_26480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5772..>6209
                     /locus_tag="GOE21_RS27045"
                     /pseudo=""
     CDS             5772..>6209
                     /locus_tag="GOE21_RS27045"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6241..6801
                     /locus_tag="GOE21_RS26495"
                     /old_locus_tag="GOE21_26490"
     CDS             6241..6801
                     /locus_tag="GOE21_RS26495"
                     /old_locus_tag="GOE21_26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067492.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            6808..7041
                     /locus_tag="GOE21_RS26500"
                     /old_locus_tag="GOE21_26495"
     CDS             6808..7041
                     /locus_tag="GOE21_RS26500"
                     /old_locus_tag="GOE21_26495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137429.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDRLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          4809..4893
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.349"
                     /coverage="100.0"
                     /evalue="1.42e-30"
     Typing          complement(4040..4582)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.441"
                     /coverage="93.0"
                     /evalue="1.54e-118"
     Typing          5554..7040
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.992"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 aacaggaaag gcaggcacgc ctggaacggg agcgtgcgcg gtcctggcat gaagagcagg
       61 aacgggaagt acgggtatgg cgtggcccgt caatggggat gtgacggtga gttgttccgg
      121 gaggttgcca gatggtaccg gaacgcgtta aaaacgcatc cgggcgcgat tctggcgtgt
      181 cgtttttgtt gctgtgtttt gcctctttgc cagatcgcgc tcattcctca gcctcatccc
      241 gcgatgtctg cgtatgtcat caactgaaaa gatggaaaaa aaacagtgtc atgaggttta
      301 cgcaggcaaa accagcgtta ttcacatggc tgaatagcta tgcatacggt taaaatttat
      361 caggtgcgat cgcggcagct tttcgggtgg tttgttgcca tttttacctg tcagcagccg
      421 tgatcgcgct gaacgcattt cagcggtgcg tacaattaag ggattatggt aaatccctta
      481 ctgtctgccc tcgtatccat cgagataaac cgcagaaatg gtgtctgcca ttagtttcat
      541 ttttttcggc gaaggcatat cgcggtttgt gtattttctg ccagcaaagg ttgttgtgct
      601 ccggagtgag tgaaaaaatt tttctgagtg gaattctttt acacgttgct gtgccgtttc
      661 tgctgatact ccggctgctt tgtctttcac ccccatatcc cggaactgct gaaagaacgg
      721 gagcatcata cgtatggtgt ctgctttctg ggtgtagaag ttaggatcaa atccatcatc
      781 tgaagcataa acataatgcc gggtatgtcg ttccaggctg gtctttggtg tgcatcctgt
      841 aagagctatt gttgctgtta tcagaggtat cagaagcagc ctggcagcgg cagatctcat
      901 aaacatctcc ttgcgttttt aatcttgata ctcagggagt tatatctccg aagagtatct
      961 cttatcatac gtcggtttac tcccaatgtg atttgatggt caatgagtca ggaataatcc
     1021 cgtgaaaaaa tgtgtgccag tcctgctttt tagtgttctt tctgcttcgg ttgccagtca
     1081 tgcggacagc aatgtttttc aacaaaaggc cgagaagaca aaaaacggca ttgttgcgga
     1141 tgttaatgcc agacagaaaa tgttcagtaa cagactgaag atacaacttc agatcgatga
     1201 gttgagagca gagctggctg gcaccagaga tgcccaaaaa agagaggatt tgcgtaaaaa
     1261 actggagtct ctggagcggc aaaagaaatc cttacgctga tgggggcgta ttttccccgg
     1321 tttcttccat aaccttttta ccgttcagat atttacgggc gaggcgaatg cgtcttgcct
     1381 ggtataccgc tgagtaagcc tgggtgggat tcacaccatt actgatttca ctgaccacat
     1441 gacgctgatc agccagtgtc agcgtgtaaa cctgtttctg gatgaggtca cgggctttgt
     1501 catactcata tccggcagca acaagggtgt cgagaagtcg tttatctgtt ttgttcagtt
     1561 tatggcgggg tgatttgctc acatcgttct ccgttttttc atgatccaac agttttatat
     1621 ctcatcctga tttgctccgg tgttctgttt cgacgttctc aatgcctgcc tccccatagt
     1681 gtgatcctta ttgcaaaaat gaaaatccat gctcttgaca cggacaatgc tgagtagtag
     1741 gttttcactg tacataaaac cagtggttat atgtacagta tattgttttt aatttattgt
     1801 tttaattatc aaagaggaat ttatggctaa agaactaagt ggatatggcc caactgctgg
     1861 tgagtctatg gggggaacag gggcgaatct gaatcagcaa ggtggaaata ataatagtaa
     1921 tagtggggtt cactggggtg gtggctctgg gcatggtaat aatggtggtc agggaaattc
     1981 aaattcatct ggttctacat caactgtgat gaaaaccggt gagtcatatc tgactccgtg
     2041 gggtgatgtt gtcattaata atgatggcct tccggtgatg aatggtattg ttatgacaga
     2101 agagaattca actctggttg ataatccatt tggaggggtc tccagagttc ttaattcttt
     2161 aattagtgat atgccatcac tttttgcaga aagctcagga aataacaaca ataacactgc
     2221 ttcagttaat actgcaccaa cgaatgctca ggtcagtgat atggataaga gttcgaaggt
     2281 agtgggcaat gtaattaatg aaaaacaaaa gcagaagaat aaaattgcta cacagattag
     2341 tgaaaaacag aagaaaatcg aggagatgaa aaaagtattc aagcatcatt cttatcatgg
     2401 tatcactgat cttgagaggg atgttgatga gttgcaaaaa aaatcaaatc agcttgatgc
     2461 agatattagc aaacttaatt cttataaaaa tacattgcaa agtaaaattg gtgatgtgaa
     2521 taaacagaag gaagctgaag aaaaggctcg tgaaaatgca gaggtcgctg aacatgaaac
     2581 tcttaatgaa gagaaacagg cggtggcgga agcagagaag cgtttagctg aggcgaaagc
     2641 tgaactggcg aaggctgaaa gtgatgtaca gagtaagcaa gctactgttt ccagagttgc
     2701 aggagagctt gaaaacgctc agaagagtgt tgacgtgaag gtcaccggat ttccgggatg
     2761 gcgtgacgtt cagaaaaaac tacagcgtca gcttgaagct aagcaggctg agtactccgc
     2821 agttgaaaat gaacttaaga atgctgttag tttcagggat ggtaaggccg ctgaggttaa
     2881 agaggcagag cagaaattaa aagaagctca ggatgctctt gaaaagagcc agataaaaga
     2941 tgctgttgat actatggttg gattttatca atatataacc gaacaatatg gagaaaaata
     3001 cgcaaaaatt gctcaggatt tagcggaaaa gtctaaagga aagaaaattc aaggtgttga
     3061 tgaagctctg gcagcatttg aaaagtataa aaatgtactg gataagaaat tcagtaaagt
     3121 tgatcgggat gctattttta atgcgttaga gtctgttact tatgatgagt gggcaaagca
     3181 tctagaaaag atctctaagg ctcttaaggt taccggatat ttgtctttcg ggaatgatgt
     3241 atgggatggt acccggaagg gattagaaac aggagactgg aggcctttat ttgtcactct
     3301 ggagaagagt gcggtagatt tcggagtggc aaaaattgtg gcattaatgt tcagttttat
     3361 tgctggtgct cctcttggtt tctgggggat tgctattatc actggtattg tttcttctta
     3421 cataggggat gatgagctga acaaacttaa tgagttgtta gggatttaat atctctttag
     3481 agtgtaatat atgaaacttg gcaatcctac tgggattgcc attgccaggt aaagtaaata
     3541 aaaaaggttg tatgagtacc aaggctcttt ttttatgttt gtcgtctttt gtataaaaga
     3601 aaatatcgtt agttttgaat atgggtatag taatacatgt attgatgcaa aaaatataaa
     3661 agctagagat atatcattgt tatagttaag tgttaaatat aataaccatg gcagaagtga
     3721 ttcaggtaaa ttatgtaggt agtattttag atacataatt ttttcttttg ttggaggtgt
     3781 atgaaaatag tgctttttct aattacaata ttatccttgt caggatgtca agcaaactat
     3841 attcgtgatg tacaaggagg aaccgttgcg ccatcttcgt cttctgaact gacggggatc
     3901 gcggttcagt agaaaagatc aaaggatctc ttgagatcct ttttttctgc gcgtaatctg
     3961 ctgcctgtaa acgaaaaaac ccgccttgtg gcgggttctt ccgtccgatc agaagtcttc
     4021 gacctcttct aatcggcggt ggtaactggc tatcgcagag cacaggtacc taaattgtcc
     4081 ttctagtgta gtcaaaatca gactgatact tcaagaactc tgttaccaac ttataaatct
     4141 cgctctgcta acccggttac caatggctgc tgccagtggc gttaagtcgt gtcttttccg
     4201 ggttggaccc aagagaacag ttaccggaga aggcgcagca gtcgggctga acggggggtt
     4261 cctgcataca gcccggcccg gagcgaactg cctaagtcgc cccgagatac cagaagacgt
     4321 gagctatgag aaagcgccac gtgtcccgta agggagaaag gcggaaaagg tatccggtaa
     4381 tcgatatggc gggaacgtgg gggcgcagga gggagcgatt caccggaaac ggtgatgatc
     4441 tttaagccac gtcgggtttc gccaccgctg gcggatttca tggttgagcc gtaaggctcc
     4501 cacagatgca ccgaaaaagt gtctgttttg tgaagtccgt caggggggcg gagcctatgg
     4561 aaaaagcgcc tccggcgcgg ccttttccgg ggtttttgtt ttttgcgcac atgttctttc
     4621 ccgggttatc cccggattct gtggataacg gggttaccgc ctttgagaaa ggagagtccg
     4681 ctcgccgcag ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaagagcacc
     4741 ggaagcggca ttttctcctt acgcatctgt gcggcatttc acacccggca tggtgcactt
     4801 ttcatacaat gcgccctgat gtcgcatgat taagccagta tacactccgc tatcgctacg
     4861 tgactgggtc agggcttcgc cccgacaccc gctaaaacct gctgacgcgc cctgacgggc
     4921 ttgtctgctc ccggcatccg ctcacaggca agctgtgtcc gtctccggga gctgcatgtg
     4981 tcagaggttt tcaccgtcat ccccgaagcg tgcgaggcag ctgcggtaaa gctcgtcggc
     5041 gtggtcgtga agcgcttcgc agatgtccgc ctggtcgtcg gcgtccagtt cattcagttt
     5101 ttccagcagg gtcagcgtct ggcttctgat aaagcgtgcc atgttaaggg cggttttttc
     5161 ctgtttagtc acagttctgt ttctccatgc gtggggagtc tgtcggaggt ttttaatgat
     5221 accggtgaaa cgcgaaagga tgctcacgat acgggtcacg gatgaggaac atgcgcggct
     5281 gctggcacgt tgtgagggaa aacaacttgc gtcatggatg cgtaaggtct gcctgggggc
     5341 accaccatcg aaaacgtcag gccttccgac actggcaccg ccgctgttgc gccagttcgc
     5401 ttcggtcggc aataacctga atcagatagc ccggaagata aacagcggac agtggtcggg
     5461 gcatgaccgg gttcatgtgg tggctgcact gatggccatc ggacgtgaac tgagtgagct
     5521 gagggatgag gtccgtaaac agggagaacg ggatgatagt taagtttcat gccaggggaa
     5581 aaggcggtgg cagtggtccg gttgattacc tgctgggccg ggagcgtaac cgggaagggg
     5641 cgagagttct gcggggtgct cccgaagagg tgcgggaact catcgatgcg acgccgtttg
     5701 cgaaaaaata cacctcgggg gttctgtctt ttgcagagca gacactgcca ccgggagagc
     5761 gtgcaagggt gatggagagc tttgagcggg tactgatgcc cggtcttgaa aaaaatcagt
     5821 acagcatcct gtgggtggag catcaggaca aggggcgtct tgagctgaat tttgtcatcc
     5881 cgaatatgga gctggtgagc ggaaaacggc tccagcccta ttacgaccgg gcggaccgtc
     5941 cgcgaatcaa tgcctggcag acactggtga atcaccatta cgggctgcac gacccgaacg
     6001 caccggagaa caggcggaca cttgtcaccc cgaataatct gccgaaagcg aaacaggagg
     6061 ccgcagaagc gattacgagg ggactggagg ctctttacca tgccggagcg ctaaaaacgc
     6121 gtcaggatgt cactgaggcg cttacagcgg caggttttga ggtggtgagg acaaccagga
     6181 gcagtatcag tatcgccgac ccggagggcg ggcgtaatct caggctgaaa ggggcgctgt
     6241 atgagcaatc ttttacaaat ggggacagac ttcgagaaga aactgaaaga gcgtgccgtc
     6301 tctacagaga acatgctgaa cagcgagttc aggaagctcg aagcgtctgt agacagggaa
     6361 ctgaacttaa acgggcagaa aatcagagac gctatcagcg cacacacagc agcggtgaaa
     6421 gaacaactgg agacgctgaa cagtgccgtg aacgcgcagt tcagcgccac agagaacgaa
     6481 ctggcccggc ggcaggaaga gctgctctgg aagctggtca aagggcggat actgtacccg
     6541 tcgctgacgg cgctttgcgt cacgggcggg atttttctgg cgtcatgggg gctgattcag
     6601 tggcaggaaa gccgtattgc ggcgaacatc ctggctatca gggaccagga agagacgctg
     6661 gcgaaactcc gggaaaaaac atggggtgtg acgtatcacg agggcaggaa cgggaaattt
     6721 ctggtgctgc cgtccggggt gaagggggaa aacaactgga cggtggagaa gaagaacgca
     6781 gtaagactgg tcagggagta agtgagtatg acagagctgg aaatgcactt actgaacgcc
     6841 ttagagcaat tgcagcagga ctatacgcag cggctgaaag actgggagag cgccttcacg
     6901 gatttgcagc atatgttcga gctgacacga caggagaacg ggcaactgag ggaacagtgc
     6961 gacgacttga gtcagcaggt gcagagatta gccgggcagg tggaccgctt gagtcggtta
     7021 tttatcacga acagcagata agggatg
//
