LOCUS       NZ_CP046423             2043 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli strain LZYWN_4 plasmid pS4-unknown5, complete
            sequence.
ACCESSION   NZ_CP046423
VERSION     NZ_CP046423.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13282710
            Assembly: GCF_017310055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Liu,Z.
  TITLE     Emergency of IncX4 plasmids ecoding mcr-1 in a clinical isolate of
            Escherichia coli from intestinal
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Liu,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2019) College of Veterinary Medicine, Northwest
            A&F University, No. 3 Taicheng Road, Yangling, Shanxi 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.48
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:59:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,717
            Genes (coding)                    :: 4,511
            CDSs (with protein)               :: 4,511
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 61 of 206
            Pseudo Genes (incomplete)         :: 148 of 206
            Pseudo Genes (internal stop)      :: 34 of 206
            Pseudo Genes (multiple problems)  :: 36 of 206
            Pseudo Genes (short protein)      :: 4 of 206
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046423.1.
            Bacteria and source DNA available from Xiaoping Song, Traditional
            Chinese Veterinary Medicine Lab,College of vetrinary Medicine,
            Northwest A&F University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LZYWN_4"
                     /isolation_source="liver"
                     /host="swine"
                     /db_xref="taxon:562"
                     /plasmid="pS4-unknown5"
                     /geo_loc_name="China:Yangling"
                     /lat_lon="34.16 N 108.02 E"
                     /collection_date="2017-05-21"
                     /collected_by="zengyuan liu"
     gene            239..1207
                     /gene="mobV"
                     /locus_tag="GJV83_RS23840"
                     /old_locus_tag="GJV83_23865"
     CDS             239..1207
                     /gene="mobV"
                     /locus_tag="GJV83_RS23840"
                     /old_locus_tag="GJV83_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_137496576.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(1269..1703)
                     /locus_tag="GJV83_RS23845"
                     /old_locus_tag="GJV83_23870"
     CDS             complement(1269..1703)
                     /locus_tag="GJV83_RS23845"
                     /old_locus_tag="GJV83_23870"
                     /inference="COORDINATES: protein motif:HMM:NF017542.2"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          231..1204
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.385"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 gcatttcacc cttgacgacg cacaaataaa acaggcttgg cagtggtggg aggtgacgcg
       61 gagcgacgcc ttccacctgc cagaccgccg ttgattttga ccttctggca gcttgctgcc
      121 cgaacgattt tgcgtagcaa agtctagtga gtacactatc atcttgatag tggcccgccg
      181 gaggtatcgc aatgcgctcc cccgcctggc tgaatgaatg ccggaatcag gggggaacat
      241 ggcgaactac gcgataatgc gctgcaaaaa gctgacggga atgggaagcg tggccagcgc
      301 gttgcagcac tgctaccgag agcgtgagac gccgaacgct gacgctgaac ggacaccaga
      361 aaactactgc tctgtgtcca aatcaacgga tcaggcaatg gggcgtgtgc gggagctgct
      421 gccggaaaaa cgccgtaaag atgcggtact ggccgttgaa tacgttatga cggcaagccc
      481 tgagtggtgg aaagaggcca caccacggca gcaggcggag tttttcgccc gctctgagca
      541 atggctggag aagaaatacg ggaaggatcg tgttgtggcg gcggtggttc accgggacga
      601 ggccaccccg cacctgtctg cgttcgtggt gccattgacg caggatggac ggctgtccgc
      661 gaaagagttt attggcgggc ggtcaaagat gcgtgaagat cagagcacct acgctgaatc
      721 agtaaaaaaa ctgggtcttg agcgcggcat cgagggaagc cgcgccacgc atcagacagt
      781 tcagcactac tacgaatcga taaaccgtgg cacccgtagc caggtatcga tctcgccgga
      841 ggcgctggag ccgcgtgtat tgcggaaggg cattttcacc aaagacgtgg aagatcaggc
      901 agccatagcc aaacgtctaa gccacgcagt gaacgatggc tttgcaggaa ccatcgctat
      961 ggcctctcag agcgcccaga acgcaaaaag agcgcgtgag ctacagaaga ctatggatgc
     1021 gcagcagaaa cgcctacaga gcgtcacaga gccttttaag gggctttcca gagagcagat
     1081 gaccgaaatc ctgatgatgg ctcagcggtt taagcagcaa aaccaggaaa aggaaaaaca
     1141 gcagcgtatt gaacgtgaaa aacagcgaca gacgcgatcg cgtggcatgg gtggtatgga
     1201 gcgttagttt tgatgcgatg caggataggg ctaacgccgc aaagtgacgt catttgcggc
     1261 attccagatt aggttctgcc gccctgtata acgctgaggc cgttttttag tgctttcttg
     1321 tggttgctga cagtaccttt ccagccaaac tgagggttca gacggtaact gtaactgcgc
     1381 cctacttttg ggccttgaag aatcacggat aggtcaatta gcgccttaac cgaacggctt
     1441 acattggttt tctgcatacc taaagcgtcg gcaatatcgg cttgggcaat ttggatgtaa
     1501 ttttcataat cgagatcagc taacagcatc atcaagactt tgaattgctc ccctgtgagg
     1561 gtcttagcaa ggatctttaa agcgtcttgg ttcatagtga aatgcctctc aaaagctgac
     1621 ttctgtttgg gtcggataac tgcgacaaaa ctacctacct cttcccctgt agcagtgtct
     1681 atctgagtaa ttcggttact catttaagag gctctcccaa aaaaatagtt atcaataatg
     1741 ataacaacgt tatcaataat gataactatc gtcaagcgag agccgcgccc cacaagggct
     1801 gagagaaggc tctcttctgt atcttatagc gagaagctca tatgcccgga tagcgaaccc
     1861 tattttcatc ggttcgtgcg taagcatttc tatgcccgtt caggtgatat tccgcttcct
     1921 cgttcaggct gttcgttcct cgctgcgctg cggtacgcct cagcctgacc tgcggcgagc
     1981 ggttcctgct ctttcaaaag cgatatccag atcctgattt cgtcaggggc gagcggagcg
     2041 agt
//
