LOCUS       NZ_CP046418            34645 bp    DNA     circular CON 18-JUN-2023
DEFINITION  Escherichia coli strain LZYWN_4 plasmid pS4-mcr1.1, complete
            sequence.
ACCESSION   NZ_CP046418
VERSION     NZ_CP046418.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN13282710
            Assembly: GCF_017310055.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34645)
  AUTHORS   Liu,Z.
  TITLE     Emergency of IncX4 plasmids ecoding mcr-1 in a clinical isolate of
            Escherichia coli from intestinal
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34645)
  AUTHORS   Liu,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2019) College of Veterinary Medicine, Northwest
            A&F University, No. 3 Taicheng Road, Yangling, Shanxi 712100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.48
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 1.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/18/2023 02:59:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,835
            CDSs (total)                      :: 4,717
            Genes (coding)                    :: 4,511
            CDSs (with protein)               :: 4,511
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 206
            CDSs (without protein)            :: 206
            Pseudo Genes (ambiguous residues) :: 0 of 206
            Pseudo Genes (frameshifted)       :: 61 of 206
            Pseudo Genes (incomplete)         :: 148 of 206
            Pseudo Genes (internal stop)      :: 34 of 206
            Pseudo Genes (multiple problems)  :: 36 of 206
            Pseudo Genes (short protein)      :: 4 of 206
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP046418.1.
            Bacteria and source DNA available from Xiaoping Song, Traditional
            Chinese Veterinary Medicine Lab,College of vetrinary Medicine,
            Northwest A&F University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..34645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="LZYWN_4"
                     /isolation_source="liver"
                     /host="swine"
                     /db_xref="taxon:562"
                     /plasmid="pS4-mcr1.1"
                     /geo_loc_name="China:Yangling"
                     /lat_lon="34.16 N 108.02 E"
                     /collection_date="2017-05-21"
                     /collected_by="zengyuan liu"
     gene            join(34592..34645,1..42)
                     /gene="dqlB"
                     /locus_tag="GJV83_RS24300"
     CDS             join(34592..34645,1..42)
                     /gene="dqlB"
                     /locus_tag="GJV83_RS24300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="WP_021534057.1"
                     /translation="MINMLIIVLRAVVAVANALIAVLELIRQFID"
                     /name="dqlB"
     gene            705..1352
                     /locus_tag="GJV83_RS23575"
                     /old_locus_tag="GJV83_23600"
     CDS             705..1352
                     /locus_tag="GJV83_RS23575"
                     /old_locus_tag="GJV83_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000587671.1"
                     /translation="MIIVIGGDKGGTGKSHLATNLTVCLSQQGKRTGLVETDLNGSTKK
                     WNKRREQAGLPPVALNEAYGDISAKITKMADVAEILIIDTAGYDSTEFRTALKVADIVI
                     VPIDPLAQVEADSLQTVTKIVRDAQKINPRLAAHVLLYKCQPNTFSEQQELRDSLNSHD
                     YWLKPMKSTVSFLRAFVRAMNQGMGVHELKSNVSGASQAKAQIELLLKELEL"
                     /name="division plane positioning ATPase MipZ"
     gene            1427..1696
                     /locus_tag="GJV83_RS23580"
                     /old_locus_tag="GJV83_23605"
     CDS             1427..1696
                     /locus_tag="GJV83_RS23580"
                     /old_locus_tag="GJV83_23605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001086090.1"
                     /translation="MAKLELPELEQNQEVNATKFVQDAGKRPTENKTKLASFRIPNELL
                     EFIDNESKRLHRPKTKIIKAALLAYQDLDENELINLWFRADRND"
                     /name="hypothetical protein"
     gene            1857..2486
                     /locus_tag="GJV83_RS23585"
                     /old_locus_tag="GJV83_23610"
     CDS             1857..2486
                     /locus_tag="GJV83_RS23585"
                     /old_locus_tag="GJV83_23610"
                     /inference="COORDINATES: protein motif:HMM:NF022440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2806 domain-containing protein"
                     /protein_id="WP_223424520.1"
                     /translation="MSLIDLSLSGLSEPGTKLIEKISDAIGVLYEPTRIRKKAKAEAEA
                     KRTELISRLELEGIEKRAVERFLKRETKRQENIENITMQAAQSLSESDNVSDIDEDWIE
                     AFFRECEDISDEQMQMLWGRILSEEAKSKGSFSRRTLKLLSTISKEEANLITYFGKFVW
                     QANKLTPILFTDENGDTEGITFDKLSVLDSLGVIQQGIGYSLRYKL"
                     /name="DUF2806 domain-containing protein"
     gene            2660..4285
                     /locus_tag="GJV83_RS23590"
                     /old_locus_tag="GJV83_23615"
     CDS             2660..4285
                     /locus_tag="GJV83_RS23590"
                     /old_locus_tag="GJV83_23615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_049589868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase MCR-1.1"
                     /protein_id="WP_049589868.1"
                     /translation="MMQHTSVWYRRSVSPFVLVASVAVFLTATANLTFFDKISQTYPIA
                     DNLGFVLTIAVVLFGAMLLITTLLSSYRYVLKPVLILLLIMGAVTSYFTDTYGTVYDTT
                     MLQNALQTDQAETKDLLNAAFIMRIIGLGVLPSLLVAFVKVDYPTWGKGLMRRLGLIVA
                     SLALILLPVVAFSSHYASFFRVHKPLRSYVNPIMPIYSVGKLASIEYKKASAPKDTIYH
                     AKDAVQATKPDMRKPRLVVFVVGETARADHVSFNGYERDTFPQLAKIDGVTNFSNVTSC
                     GTSTAYSVPCMFSYLGADEYDVDTAKYQENVLDTLDRLGVSILWRDNNSDSKGVMDKLP
                     KAQFADYKSATNNAICNTNPYNECRDVGMLVGLDDFVAANNGKDMLIMLHQMGNHGPAY
                     FKRYDEKFAKFTPVCEGNELAKCEHQSLINAYDNALLATDDFIAQSIQWLQTHSNAYDV
                     SMLYVSDHGESLGENGVYLHGMPNAFAPKEQRSVPAFFWTDKQTGITPMATDTVLTHDA
                     ITPTLLKLFDVTADKVKDRTAFIR"
                     /name="phosphoethanolamine--lipid A transferase MCR-1.1"
     gene            4357..5079
                     /locus_tag="GJV83_RS23595"
                     /old_locus_tag="GJV83_23620"
     CDS             4357..5079
                     /locus_tag="GJV83_RS23595"
                     /old_locus_tag="GJV83_23620"
                     /inference="COORDINATES: protein motif:HMM:NF028536.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family protein"
                     /protein_id="WP_039003037.1"
                     /translation="MPYLSNKRLLAEMSIALVMAIVATLTLEHSQIDLMVADWFYLGMG
                     HWMVAKQAFLPDLLLYSGLKKLLMAMLIYLLVATICRAYHEKKGNAITAKWLVPVTKFR
                     VRELAYLVLTLILVPTVVASLKAYTHVVCPVHLTIFDGTLPYLPMLDSMRNTIPDKCFP
                     AAHASSGFALFAFAFAPSLRRRRGAIIIVVMALGWAMGCYKMIIGDHFLSHTVVSMMLA
                     WAMSAGLAWVFFKKGEQV"
                     /name="PAP2 family protein"
     gene            complement(5470..5997)
                     /locus_tag="GJV83_RS23600"
                     /old_locus_tag="GJV83_23625"
     CDS             complement(5470..5997)
                     /locus_tag="GJV83_RS23600"
                     /old_locus_tag="GJV83_23625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="WP_000577043.1"
                     /translation="MIFNRNKKRDEQHNSAVVLPSAENVFSDFIKNNPALTTNAFTAIG
                     EVDCFTKVPLLSNNESEFLGILSRNIIPDYSVFPKIRLIEFVRPHGSEEAVKDLIREVQ
                     NITCDFVLKSYDETVLAVIQFGETATVRQQKKKLIIQRVCAMTGISFFEFKNTVDMMGS
                     EDFCQFLRDDEE"
                     /name="DUF2726 domain-containing protein"
     gene            complement(6132..6536)
                     /locus_tag="GJV83_RS23605"
                     /old_locus_tag="GJV83_23630"
     CDS             complement(6132..6536)
                     /locus_tag="GJV83_RS23605"
                     /old_locus_tag="GJV83_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012421203.1"
                     /translation="MKDNIVDFQRIRDSMNDHSTDVANDAVYSDKKKISRFFYLLTPVR
                     YLLAASLYLVVVIPLGILAGFRFLLQFLGGIAVMGLTIAWYMGADVSSYVVLAAWIALT
                     VCGCAEQIFEWWIESRFAFRLFGIGRTPEE"
                     /name="hypothetical protein"
     gene            complement(6549..7415)
                     /locus_tag="GJV83_RS23610"
                     /old_locus_tag="GJV83_23635"
     CDS             complement(6549..7415)
                     /locus_tag="GJV83_RS23610"
                     /old_locus_tag="GJV83_23635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473366.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001353743.1"
                     /translation="MTTNKTSLSRLTKVRHRNELNSTLSTLPMAAKKVLFLAMCQINSK
                     NEFDDDHIFYVTVADYIKWVQVKPDAAYLALRDGSNILDTTLLKLKHDEILELSSDLGF
                     KFTKSNVPDSMNLSLTVFSTYYRNEGRVGIKFTKEAKRFLCKLIGEEKRYTTQVLLSVV
                     RLTSVNAASLYQLIRKIYSNNSRANSFEMTIDELKDELNLYTIGAGGVKDYKYPDYPAF
                     KRDVLNKSVKEIMKHTEVKNLSFVVSEKIGRKVYKLKFSYTIGYEGDTREDSEFTNMFD
                     KMYPPEN"
                     /name="replication initiation protein"
     gene            8830..9345
                     /locus_tag="GJV83_RS23615"
                     /old_locus_tag="GJV83_23640"
     CDS             8830..9345
                     /locus_tag="GJV83_RS23615"
                     /old_locus_tag="GJV83_23640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /GO_process="GO:0006457 - protein folding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="WP_001025395.1"
                     /translation="MNIQEALNVFGLSGELTEKDIKAAYRKAALKYHPDRNPLGAELMK
                     AVNAAFDVLMANIDKINQFQSTDEHARYNYGDDLEKVLNVLSGLSGLVFEVIGNWVWIS
                     GETITHKETLKEIGCKWAAKKKQWFYRPDEHKSYWNREEHTIEEIRAKYGTTGQRRATG
                     WQRVETRA"
                     /name="J domain-containing protein"
     gene            9450..9992
                     /locus_tag="GJV83_RS23620"
                     /old_locus_tag="GJV83_23645"
     CDS             9450..9992
                     /locus_tag="GJV83_RS23620"
                     /old_locus_tag="GJV83_23645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001083906.1"
                     /translation="MNSFFEQYHPVFEVVCRILGNGWRVNKLDDCSSRIKLTSPQFKNY
                     SVHIRMEKDRFSVVGSVDSRSWRSPHHVCTLSRKRNPVDIAADIERKILVNASQEVLQA
                     IEYEKHQVEKKDEILILKGMLSQLVQLESWYGALTGFKAENGLNGKVTEQGDSYDLQIR
                     GLSIDQLVKITGYLKQL"
                     /name="hypothetical protein"
     gene            9989..10240
                     /locus_tag="GJV83_RS23625"
                     /old_locus_tag="GJV83_23650"
     CDS             9989..10240
                     /locus_tag="GJV83_RS23625"
                     /old_locus_tag="GJV83_23650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001565860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001230707.1"
                     /translation="MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDA
                     VLNENELQHIDFTCAGLREDDETPVKNLIKEFMLNETR"
                     /name="hypothetical protein"
     gene            10227..10454
                     /locus_tag="GJV83_RS23630"
                     /old_locus_tag="GJV83_23655"
     CDS             10227..10454
                     /locus_tag="GJV83_RS23630"
                     /old_locus_tag="GJV83_23655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000801440.1"
                     /translation="MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQN
                     SQSLSLVEFEFLLLLAGEHPEYVLCKRESK"
                     /name="hypothetical protein"
     gene            10908..11207
                     /locus_tag="GJV83_RS23635"
                     /old_locus_tag="GJV83_23660"
     CDS             10908..11207
                     /locus_tag="GJV83_RS23635"
                     /old_locus_tag="GJV83_23660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000650309.1"
                     /translation="MKCSSVFTSTTNHVFTFERVTLCTIILMHKDTGQQYVVIFTDNNK
                     IRDYKAGIVPQFGELKQSDVDLVLFYRDEYEKYFDSLKDGDECLSFKDFIECLC"
                     /name="hypothetical protein"
     gene            11298..11648
                     /locus_tag="GJV83_RS23640"
                     /old_locus_tag="GJV83_23665"
     CDS             11298..11648
                     /locus_tag="GJV83_RS23640"
                     /old_locus_tag="GJV83_23665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000854259.1"
                     /translation="MKTLKQAAMQFASDLRNNRCFKAAYNDALLELDREDLNAIVQCTF
                     LFEPKRTLAELDKLIKKIEVEEDYEFCVSLDKGKGLHEVRTFKSEREALDAYRILTLYG
                     YTVTIEKKEGND"
                     /name="hypothetical protein"
     gene            11953..12201
                     /locus_tag="GJV83_RS23645"
                     /old_locus_tag="GJV83_23670"
     CDS             11953..12201
                     /locus_tag="GJV83_RS23645"
                     /old_locus_tag="GJV83_23670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653200.1"
                     /GO_function="GO:0003729 - mRNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family toxin"
                     /protein_id="WP_000520549.1"
                     /translation="MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFF
                     NAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAGLL"
                     /name="type II toxin-antitoxin system HicA family toxin"
     gene            12198..12545
                     /locus_tag="GJV83_RS23650"
                     /old_locus_tag="GJV83_23675"
     CDS             12198..12545
                     /locus_tag="GJV83_RS23650"
                     /old_locus_tag="GJV83_23675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="WP_000681613.1"
                     /translation="MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQ
                     EFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPELHRRVAEMAMEEDLSLNAFVNKAL
                     EKEVSNHRAGA"
                     /name="type II toxin-antitoxin system HicB family
                     antitoxin"
     gene            12906..13409
                     /gene="taxA"
                     /locus_tag="GJV83_RS23655"
                     /old_locus_tag="GJV83_23680"
     CDS             12906..13409
                     /gene="taxA"
                     /locus_tag="GJV83_RS23655"
                     /old_locus_tag="GJV83_23680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxA"
                     /protein_id="WP_000781813.1"
                     /translation="MKMIAFRLSDDELKFAEHNAILSGFTSINAFAKHNVLNIETKPVN
                     IPVNNEPAKLVSVRLYPHEIELVKRNAALHGMSMSREIAIRVRQSLLKSEVCLYPDEVK
                     ELKKLSTAVDRVGRNIHFIIKGERFCTVNDPDFRKDVIEVIELCKQIDSKLETLTKSVV
                     NRFG"
                     /name="taxA"
     gene            13413..14642
                     /gene="taxC"
                     /locus_tag="GJV83_RS23660"
                     /old_locus_tag="GJV83_23685"
     CDS             13413..14642
                     /gene="taxC"
                     /locus_tag="GJV83_RS23660"
                     /old_locus_tag="GJV83_23685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA relaxase/nickase TaxC"
                     /protein_id="WP_001329504.1"
                     /translation="MGVYVEQEYRIKRAKGTGRDPKSPKLSGRHIHASKSAFNHKVRHG
                     ADKKAYTWTPTKEVTFKITGSGKTAAGIKNGIDYITRNGELEAYCYDGKGSEQTGKGED
                     FNREFTSTLSKGNDYSRTYRGENIDHVKNMVFSPPPEAGVSREDALKATVEFLKETYPN
                     HAFVAVYHDDKEDHPHVHVNIKLRDEETGRRLRLTKSETRKFRNGFHRKLKGMGYDVTA
                     TWKKDPERKREIERLQAENPKRLRNVYKVVDFGETSYQNKAGEKRTPFLTYETLKGGKQ
                     VTIWGKDLKNHFESEKLQPGTLIKVKKLAPTLVRSPMFNDDGTVAGYRETHRNNWQIEN
                     IALERNRERQVHERETRQPEESDVKKQLSRKHEQGHNIGFALEHGFTKDSEEHKKLRIQ
                     QERNWKGLGF"
                     /name="taxC"
     gene            14719..15012
                     /locus_tag="GJV83_RS23665"
                     /old_locus_tag="GJV83_23690"
     CDS             14719..15012
                     /locus_tag="GJV83_RS23665"
                     /old_locus_tag="GJV83_23690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134733.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_060598542.1"
                     /translation="MTEHDAICISGLHQIFSDEEHLSEQQKDIILMYAYGYTLNEIADF
                     KGLKPSTVRKYLDSVRAELGGVSLAGIRTLVLIRTNALLVSSLSRISERGNL"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            15009..15224
                     /locus_tag="GJV83_RS23670"
                     /old_locus_tag="GJV83_23695"
     CDS             15009..15224
                     /locus_tag="GJV83_RS23670"
                     /old_locus_tag="GJV83_23695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="WP_000760375.1"
                     /translation="MKKYLLFALPFFVVGCSEEVKSVDWWGQHLTEAKQKQAECEKSGS
                     DSQNCKNVKQALFIQSQKDAPVPTFD"
                     /name="EexN family lipoprotein"
     gene            15580..16200
                     /locus_tag="GJV83_RS23675"
                     /old_locus_tag="GJV83_23700"
     CDS             15580..16200
                     /locus_tag="GJV83_RS23675"
                     /old_locus_tag="GJV83_23700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing protein"
                     /protein_id="WP_000157549.1"
                     /translation="MASLFPALLLCAASVHPDTINDIARVESGFNPYAVAEIIPERERG
                     SYGKSVISHMPKSKEEALTVIREIENRKRRYSVGLMQITSSNFSFYSTSAEKLLDSCEN
                     LSVFEKIIVDCYKRGRSLENALSCYYTGNFSNGKRKEKEFNNTSYVERIGYTGNEKKYV
                     VPGTRSNGGEQRKNRSHNASVIWPETILKSAFVDNSHPTKVIN"
                     /name="lytic transglycosylase domain-containing protein"
     gene            16214..16540
                     /locus_tag="GJV83_RS23680"
                     /old_locus_tag="GJV83_23705"
     CDS             16214..16540
                     /locus_tag="GJV83_RS23680"
                     /old_locus_tag="GJV83_23705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="WP_000008647.1"
                     /translation="MSFKKSVCNRISSSVTAVMQRKNKVVMAVAGATVASPAFADGFSK
                     AETLLEKVKTGLSGLSLVTVTIACLWVGYKVLFGGSTIRECSPIIIGAIVIASAAEVAS
                     MMVN"
                     /name="TrbC/VirB2 family protein"
     gene            16559..19306
                     /locus_tag="GJV83_RS23685"
                     /old_locus_tag="GJV83_23710"
     CDS             16559..19306
                     /locus_tag="GJV83_RS23685"
                     /old_locus_tag="GJV83_23710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
                     /protein_id="WP_000105985.1"
                     /translation="MSTLYKAMTRPAMYVGVPVVPLTVVAGALFLAGVYISKLIWLAIP
                     VAVFVLRMITKQDDHIFNLYFLKLKMLGNSVCNRFFGARAFLSGQYEAVEIDEFVNAMK
                     LNERITTGKYIPYSSHVDKNIVKTKNGDYVATWQLMGINFESISAEMLETIDSQVATLV
                     RSFSGLPVSFYNHSCRASFYDAFTTKSGNKYADIISDCYYGSMKKNKFKGNTLYFTLIY
                     RPDGRVEKLEKRKKSIKEKKDDINIHVKRMNEMINTFSGALDKFTCKLLGMYEENGKVF
                     SSQLSFYNYLLTGKLQKIRVTDSPVYNVLGGVDVFFNHDTGQICRIDGNKFFRSIEIKD
                     FCSETASGVFDVLQYSDADYIITHSYTSMSKSEALSTIKRAEKQLKSTEDDAVTQLQEL
                     EKAKNDIVSGDISFGYYHFTLMVMADSIRELDESVSKITADFTDLGIIPALSTMSLPAA
                     YFAQLPAVFHLRPRLSPVSNVNFVELASFHNFYQGKRDKNCWTEAVAILKTPSKQAYYL
                     NLHNSVLFKDETGEKNLANTKVIGTAGSGKTMFLSYLACSLQKYNNPETFADSAKNKKL
                     TCVFLDKDRGAELCIRMLGGEYYTVKSGEPTGWNPFALEATKRNRIFVKQLMEILCTRN
                     GERLSTRERLLISESVDAVMDFPPGEMREYGITRMLEHLMQRDDRDEQENGIILRLSQW
                     ANGQAHGWVFDNAKDTFNIQHVNNFGIDGTEFLDDPMVCAPITFYLLYRITQLLDGRRL
                     VIFLDEFWKWLQDEAFSDFVYNKLKTIRKLNGLVIPATQSPDEILKNKISRAVVEVCST
                     SIYLANPDADYNDYVEGLKLTPEEFNIVKNLDPMSRQFLIKKSSLKKGDGKSFSALATL
                     DLSGLGGYLKILSASADNLEIFESIYHEGMEPDDWVPEYLERAI"
                     /name="VirB4 family type IV secretion/conjugal transfer
                     ATPase"
     gene            19318..20034
                     /locus_tag="GJV83_RS23690"
                     /old_locus_tag="GJV83_23715"
     CDS             19318..20034
                     /locus_tag="GJV83_RS23690"
                     /old_locus_tag="GJV83_23715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_206970428.1"
                     /translation="MKRVKTLMLISLLTASFYSSAGIPVAIDASPEWAVEATRWTERLK
                     QWSETARHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELANIYKQGNSYISDFTS
                     NPEGALSSQAKNLFSKYGAFNMCSTGIESSDNLCKARIVSTAASIEQGNEINKQLSSAM
                     SQIQSLSNRIESSKDMKESQDLANALQAQSLKIQAIKMQYDVWDSKNKADHEMLVTQEQ
                     EAFIKQQKEAEPLTFD"
                     /name="type IV secretion system protein"
     gene            20046..20726
                     /locus_tag="GJV83_RS23695"
                     /old_locus_tag="GJV83_23720"
     CDS             20046..20726
                     /locus_tag="GJV83_RS23695"
                     /old_locus_tag="GJV83_23720"
                     /inference="COORDINATES: protein motif:HMM:NF016491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_251361728.1"
                     /translation="MAQGFFVKYNSTVMDSVDKISSSYQTQFANDIMSLATVSVTLYVL
                     WKGYQILASKTQTPLQDLVWDLSKFAIIIMFITNADGYLTAATDALQGMKDGFSGGVSV
                     WQTLDNLWKSTQNLGAEIYSLDKSTYVKDQGVVGQFLIWTGSLILMAVSVVVFLTADVT
                     MKLLIITAPIFIFCLMFGFIRSMFNNWLQSLFSSILTVLFASLVIRIAMDFQGMILSHA
                     RLAP"
                     /name="type IV secretion system protein"
     gene            20802..22030
                     /locus_tag="GJV83_RS23700"
                     /old_locus_tag="GJV83_23725"
     CDS             join(20802..21117,21117..22030)
                     /locus_tag="GJV83_RS23700"
                     /old_locus_tag="GJV83_23725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_089541817.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAASQ
                     LFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVEYG
                     RGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWKDGRRSRHSDDTDVLLR
                     IHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKTAVPPSKR
                     AHTGKVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFDSETVQ
                     DVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGI
                     AESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQQASN
                     GLSDNRCLEI"
                     /name="IS3 family transposase"
     gene            22105..22476
                     /locus_tag="GJV83_RS23705"
                     /old_locus_tag="GJV83_23730"
     CDS             22105..22476
                     /locus_tag="GJV83_RS23705"
                     /old_locus_tag="GJV83_23730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251361729.1"
                     /translation="MAGFLGALLVLIAKGFAVQLAGAGVEGAVQGAAMMGLGAAGMATG
                     KSLMLGGRAGLGFGLGMAGRTGMNSLSGKAGNLMGRGARTAAEWAGEKGYQALAPTGAL
                     GMKARRLASLEKARARNAA"
                     /name="hypothetical protein"
     gene            22991..23677
                     /locus_tag="GJV83_RS23710"
                     /old_locus_tag="GJV83_23735"
     CDS             22991..23677
                     /locus_tag="GJV83_RS23710"
                     /old_locus_tag="GJV83_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="WP_001290765.1"
                     /translation="MSEKIEKKIETAKSFERQLYAENERSKKNAWRVAVAASLLSLCLG
                     AAICVLVPLKEKELAIVAVGEKTGRTELITQVKQEKILQSEALGRYFVNTYITLREGYN
                     YPSLQYDYETVQLYSSNTVKDDYLRLYNSDMAPDKIYHNNGSYVAVEVISNIISDATAP
                     DKLASVRFKKTTRNFTTGQVSVSYWTARVTYRFEPEKSVKSSSRELNPLGFTVTSYQTD
                     REVRGE"
                     /name="type IV secretion system protein"
     gene            23674..24582
                     /gene="virB9"
                     /locus_tag="GJV83_RS23715"
                     /old_locus_tag="GJV83_23740"
     CDS             23674..24582
                     /gene="virB9"
                     /locus_tag="GJV83_RS23715"
                     /old_locus_tag="GJV83_23740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein VirB9"
                     /protein_id="WP_001329500.1"
                     /translation="MMKKLFIATCLLLPGLVYSAATPLPSGFDARMQTVSYNGANTTVI
                     RSKTGFLTSVVFDEGEAVISAKAGFPAGWEITTDDNVVYINPRPVVQEQEGDEGEKLKK
                     VFQPTEKEWDTNLFVRTTKRIYSLDLILLSEEKQAQPAYVVQFRYPSEIAKKNAEEVRL
                     AKEKQEKLRQKKLISESFEKADAPKNWNYFMRVNEKYDSRRIAPDFAYDNGIFTFLGFN
                     SGKVFPAPFAVRDGQEQTLAFNVETKGKYKIMVIHNVNDKFVLRYGNSVVGVVNKSFGK
                     VLTDQRNTSSPAVERVEVNND"
                     /name="virB9"
     gene            24575..25693
                     /gene="virB10"
                     /locus_tag="GJV83_RS23720"
                     /old_locus_tag="GJV83_23745"
     CDS             24575..25693
                     /gene="virB10"
                     /locus_tag="GJV83_RS23720"
                     /old_locus_tag="GJV83_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB10/TraB/TrbI family type IV secretion system
                     protein"
                     /protein_id="WP_000131271.1"
                     /translation="MTDMKKTDDLVDDANQSKLESKPVHDIGDIRNKNNRMRSGFLFGL
                     MAVLVIGIFALKTYKNYFADDQQKASESTGDTSISQVSKIRTGLGQNFDPVENKAIINP
                     GATGSGNTVSDGHKEVPQEGFRKYLSIPVAGQGGSQTGASGSSRTSQTSEPQEERKESK
                     ENVPGKSGMKVTAINLDPDLYIEENRLIPCALTTRFVSDVAGRISCVFTEDVWSANHHT
                     KLLEQGTKAFGRYQTGTLNHGQGRMFVMWEQLRTPDNKRIDMVNTAAAGPLGEAGIDGW
                     IDSHFWERFGGSLMLSMVQDVAAAAADNAPGKDRNVDYTENSRQAMAEMAKVALENSIN
                     IPPTMYKNQGDIITIMVGEDIDFSDIYELKVK"
                     /name="virB10"
     gene            25695..26723
                     /gene="virB11"
                     /locus_tag="GJV83_RS23725"
                     /old_locus_tag="GJV83_23750"
     CDS             25695..26723
                     /gene="virB11"
                     /locus_tag="GJV83_RS23725"
                     /old_locus_tag="GJV83_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401678.1"
                     /GO_component="GO:0043684 - type IV secretion system
                     complex [Evidence IEA]"
                     /GO_process="GO:0044097 - secretion by the type IV
                     secretion system [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type DNA transfer ATPase VirB11"
                     /protein_id="WP_000017226.1"
                     /translation="MSGVIPVSISNKSLDFYKNQVFQSFLDIEGLTEIAVNRPGEIWTE
                     INGDWTFHNNDSVTFDFCKRFSHTLASFRGDEIGETKPLLSATLESGERVQVVFPPACE
                     RNTISITIRKPSTRQITHKEYVANGFYDYVQSGKKHRTYDDELLDLFRARNIAAFMELA
                     VAAGKTIVFAGATGSGKTTYMKSLIDFIPLNTRLITIEDAEEIKFFIHKNYVHLFYPSE
                     SGSDSGSIITAAKLIKSCLRMKPDRILLAEIKGGDAWDFVKVAGSGHSGSMTSIHAASA
                     KDAIIQMVTKCYQNRECQNLPFDVLRKIIMDSIDIVVHVGRDGVVRHMSDIYYKGAECE
                     NF"
                     /name="virB11"
     gene            26710..28572
                     /gene="taxB"
                     /locus_tag="GJV83_RS23730"
                     /old_locus_tag="GJV83_23755"
     CDS             26710..28572
                     /gene="taxB"
                     /locus_tag="GJV83_RS23730"
                     /old_locus_tag="GJV83_23755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome-coupling protein TaxB"
                     /protein_id="WP_170324155.1"
                     /translation="MKTFNKSQSVFIFAVMLVAAWFAGSFIFFVLYFSQIKNLKPLKAV
                     FRSIDAYRMDIFTDSLTLSVVTTDIRVLAFVALGAGFVVSLIIPVAALIKLNEKKENIF
                     GDARFATINDIRESNSFTLDGDEKDGIIVGIKDKKIIRYVGAAFSAMGAGTRAGKGAGI
                     VITNLMKYWWSVIILDPKRECFNITSLIRKVILGHEVYKFDPFSSVTHRFNPLYYVSMG
                     TSEGFNQLENLSLIIYPYKTDGADAGSYLNKTAGGVFKSYAVALWFMIKNDKAGLKTLD
                     IEPVFSMSKINQLFERAEPEHLLSFMNDIRSELRGKDKTLADIGVAGLKAFIEMEDKTK
                     AQLKSNFLNGLSPFANPNVANATDGNDFDLRQVRKKRMTIYFCISGDNARLAEKITNIF
                     FQLAIQVNLEKMPTDDPEIKHDCLFLLDEFPSIGAVDYIKSKSGLIAGYKLKLLIVYQV
                     GSQLEEIYSYAGSKTLLASAPCKIVYSASDVKDARELSEAMGTRTVTIGSKSKSRSRGG
                     TSRSESESLIERPLVTTNELLTLKFSEEILAMKGENPIRCEKAFYYLNEYFFGDFVKVA
                     PELANIFPRKKGKLVMPPQKVFENEIVAKGYLVVKDVPDLDKAA"
                     /name="taxB"
     gene            28581..29003
                     /locus_tag="GJV83_RS23735"
                     /old_locus_tag="GJV83_23760"
     CDS             28581..29003
                     /locus_tag="GJV83_RS23735"
                     /old_locus_tag="GJV83_23760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cag pathogenicity island Cag12 family protein"
                     /protein_id="WP_015059903.1"
                     /translation="MMKKLVFLLISILAGCSSPPEPTPVQFEKANEVINPSLPYVPDFH
                     GVIKSDVSGKGWVYEITSLSGVQARTPTFYYALAHADRIVVTTHDAGLWFRIRDLLKME
                     GATAVVEWRNEKSFLPEQVKIVFIKAQNEVKNDWKK"
                     /name="cag pathogenicity island Cag12 family protein"
     gene            28987..29292
                     /locus_tag="GJV83_RS23740"
                     /old_locus_tag="GJV83_23765"
     CDS             28987..29292
                     /locus_tag="GJV83_RS23740"
                     /old_locus_tag="GJV83_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="WP_001350475.1"
                     /translation="MTGKNRVVLPALLALGLISTNANASDPCASVLCLYGKAVGQGGGS
                     ECRSAEKDFFNILKKKKGSIRWSKTFDARKAFLNQCSTADPAAISKIMSKFGRVRG"
                     /name="TrbM/KikA/MpfK family conjugal transfer protein"
     gene            <29405..29599
                     /locus_tag="GJV83_RS23745"
                     /pseudo=""
     CDS             <29405..29599
                     /locus_tag="GJV83_RS23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517490.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /name="ATP-binding protein"
     gene            29603..30175
                     /locus_tag="GJV83_RS23750"
                     /old_locus_tag="GJV83_23770"
     CDS             29603..30175
                     /locus_tag="GJV83_RS23750"
                     /old_locus_tag="GJV83_23770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001198960.1"
                     /translation="MQYALFDGFERKFLLDALEFGVLKDWKENPVKELPDIDESAHPFH
                     ICYGGYLLNPGVSDSDISRKIKDQAGFWLAAIDDTRMDCHSIAYYDIHTLPLISCGHQK
                     IVPFAALIKADECIISKIASYSGFAVTAFLRIKEWDIATNILNREGIFAFNGCERRFRV
                     VSKDNWQHTVSEERAIRCAKRLIQCKG"
                     /name="hypothetical protein"
     gene            30180..32333
                     /locus_tag="GJV83_RS23755"
                     /old_locus_tag="GJV83_23775"
     CDS             30180..32333
                     /locus_tag="GJV83_RS23755"
                     /old_locus_tag="GJV83_23775"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247728.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003916 - DNA topoisomerase activity [Evidence IEA]"
                     /GO_process="GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="WP_001235745.1"
                     /translation="MRLFIAEKPAVANDIVKALGGNFTRHDGWFESDNAIVTNCFGHII
                     ESQPPENYNPEYKAWKVETLPLRLYPVKYQPVESAAKQVKTILELIRRGDVTEIVHAGD
                     PDDEGQLLVDEVLEYAGNTKPVKRVLINDNTLPAVKKALANLKDNRDFKGLYLKALARS
                     VADAVYGFSMTRAYTIPAKARGYQGVLSVGRVQTPVLGLIVNRTRANQNHKSSFYYTMT
                     GVFQRGADVIRANWKPGEFAPLTDRKLLDKAWADGTAASLAGKPATVEAAATDDKKTAA
                     PLPFNLVRLQQYMNKKFKMTAQKTLDITQQLREKYKAITYNRSDCSYLSDEQFSEAPQV
                     IDALKSVFPQSLDIDSARKSKAFNSAKVTAHTAIIPTASVPDVNALSTDERNVYLAIAQ
                     HYLVQFMPEKAYQEVSVAIQCGDESFYARARKTTDSGFEAFLGAETTDEGESEDNDDSA
                     FELLCKIRTGETLTTKEVIVNEKKTTPPPLFTEASLLAALVRVADFVTDPTIKKLLKDK
                     DKDKKDEHGGIGTPATRAAILETLKKRNYITLEKGKLIPTDTGYALIDALPGIAVNPDM
                     TALWSEKQAAIENGDLTVEQFINDLYGELTGMISDVDLGEMKIEPAAPAGQFQRLDSPC
                     PSCGKHIVIRPKGYFCTGCEFKIWSEFSGKKITQAQAEKLVKSGKTDLIKGFKKKSGGT
                     YDTVLVLEDKKTGKLGFPARAKK"
                     /name="type IA DNA topoisomerase"
     gene            32330..32536
                     /gene="hha"
                     /locus_tag="GJV83_RS23760"
                     /old_locus_tag="GJV83_23780"
     CDS             32330..32536
                     /gene="hha"
                     /locus_tag="GJV83_RS23760"
                     /old_locus_tag="GJV83_23780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="WP_000850859.1"
                     /translation="MKTKQEWLFQLRKCTSRDTLEKVIEINRYKLPLSESEAFYSAADH
                     RRAELVMNKLYDKVPSGVWKYVH"
                     /name="hha"
     gene            32554..33018
                     /locus_tag="GJV83_RS23765"
                     /old_locus_tag="GJV83_23785"
     CDS             32554..33018
                     /locus_tag="GJV83_RS23765"
                     /old_locus_tag="GJV83_23785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293133.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS family nucleoid-associated regulatory
                     protein"
                     /protein_id="WP_001293134.1"
                     /translation="MSELTKEDEYGIISRTMMNIRSLRVFAREIDFEQLLEMQEKLNVV
                     IEERREDAEREAAERQELEAKRQQAIEYIISLGLDPESLLAPVTADTVKTRRKAKGGVR
                     KAKYRFKDENGEIREWSGNGKRPLALQKLLDEGHFMEDFLIEKTKPEQAE"
                     /name="H-NS family nucleoid-associated regulatory protein"
     gene            33105..33809
                     /locus_tag="GJV83_RS23770"
                     /old_locus_tag="GJV83_23790"
     CDS             33105..33809
                     /locus_tag="GJV83_RS23770"
                     /old_locus_tag="GJV83_23790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     ARG             2661..4282
                     /name="mcr-1.1"
                     /gene="mcr-1.1"
                     /gene_name="phosphoethanolamine--lipid A transferase
                     MCR-1.1"
                     /tool="amrfinderplus"
                     /drug_class="COLISTIN"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(6550..7421)
                     /name="IncX4"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          13414..14639
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.511"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          20047..20711
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.55"
                     /coverage="99.0"
                     /evalue="1.0900000000000001e-130"
     Typing          22043..22458
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.561"
                     /coverage="99.0"
                     /evalue="6.960000000000001e-29"
     Typing          22992..23674
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.4399999999999998e-137"
     Typing          23675..24579
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="99.669"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          26711..28569
                     /name="MPF_T"
                     /typing="mate-pair-formation"
                     /tool="MOB-suite"
                     /identity="98.71"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgattgcag ttctggaact gatccgccaa ttcatcgatt gatgacaacg gaaacgtaac
       61 taagggcagg aagtgaaccg cttcctgccc tttaacaaaa aaggaggatt tatggatttt
      121 atgagcattt tggggcgctg tttacttctt cctttccctg ttgcgatgtt tgtcggttgc
      181 ctgtttcctg gcatcgatga tcgcttatcc ggaatgctta tgagtgttgc tgtgggatgt
      241 ttatcgtggt atgtcaccag accgaagaac cgacagacaa aggccgcgta agtggccttt
      301 aattgggata ccctttagtt acaacattct aatagctcat tccatcctcc gctcatccag
      361 caaacataac ctccctgccc ctccccacta cagaaagtgg cgcgccccgc ccgtacgggc
      421 ggaatctaat cgcttaattt tgaatgttgt aactaaacat actcattgct gtattgccac
      481 aaggtttcgc agcaagggag gataccgaaa gtaacgctgt cacttttgct ttcaagtgac
      541 caaccaacgg gtacatcaca ctgacataca ataatgtatt tcttcatgat gtacatcatt
      601 gtaattcatt gtgacataca ctatagtatg tccatgtgac atacgcatgc gtacatcaca
      661 tagaaataca catgagtaaa tcactgtgac atacaagggg tactatgatt atcgtaatcg
      721 gtggtgataa aggcggtaca ggtaaatcac atctggcaac caacttaact gtttgcctct
      781 cacaacaggg aaagagaacg gggcttgttg aaacagacct gaatggctcg accaaaaaat
      841 ggaataaacg cagagaacag gccggattac cgcccgtcgc attaaatgaa gcctatggcg
      901 acatcagtgc caaaataaca aaaatggcag atgtagcaga aatcctcatt atagatactg
      961 ccggatatga cagcacggag ttcagaactg cactaaaagt tgcagacatt gtgattgtcc
     1021 cgatcgaccc gcttgcccag gtggaagcag acagtcttca gactgtgaca aaaattgtca
     1081 gggatgcgca aaaaataaat cccaggctgg ctgcacatgt gctcctgtat aaatgccagc
     1141 caaatacgtt cagtgaacaa caagaattaa gagactctct caacagccat gattactggc
     1201 ttaaaccaat gaaaagcacg gtttcttttc tccgggcctt tgtaagagca atgaatcagg
     1261 gaatgggggt tcacgagtta aaaagtaatg ttagcggtgc cagccaggca aaagcacaaa
     1321 tcgaactctt gctgaaagaa ctggaactgt aaatcatagt gacatacact aatgtacatc
     1381 atcatgatat acattagtgt gaaacaatca gattagaagg tacatcatgg caaagctgga
     1441 actaccagaa ctagaacaaa accaggaagt gaacgccaca aaatttgttc aggacgcagg
     1501 aaaacgcccc acagagaaca aaacaaaact ggcttcattc cgcataccga atgaattgct
     1561 tgaattcatt gataacgaat caaaaagact gcatcgtccc aagacaaaaa taatcaaagc
     1621 agcattactg gcttatcagg atctggacga aaacgaactc attaatttgt ggttcagagc
     1681 tgacagaaat gattaatcat agcaagtggc ccaataaact ctttatcatt ttcttttaga
     1741 tacttatctg aatgtactaa cttcatcact tttaaatgta gttgtgattc atttttagtt
     1801 actcttgcaa taaaataccc ttgtacagtt gttgcacggg tatttaggag cactagatga
     1861 gccttatcga tttaagttta tcaggattat cagaaccagg aacaaaactt attgaaaaaa
     1921 taagtgatgc tattggtgtg ctttatgaac ctacaagaat caggaaaaaa gcgaaggctg
     1981 aagctgaagc taaacgcaca gagctaattt ctaggttaga gcttgaggga atagaaaaac
     2041 gggcagttga gcgttttctc aaacgtgaaa cgaaacgaca ggaaaacatt gaaaatataa
     2101 caatgcaagc agcacaaagt ctatctgaga gtgataatgt ttctgatatt gacgaagatt
     2161 ggatagaggc tttcttcagg gagtgtgagg atattagtga cgaacaaatg caaatgcttt
     2221 ggggaagaat tttatccgaa gaagcaaagt caaaaggttc gtttagtcgt agaaccttaa
     2281 aactattatc cactataagc aaagaagaag cgaaccttat tacttatttt ggaaagtttg
     2341 tttggcaggc aaacaaactc actccaattt tatttaccga tgagaatgga gatactgaag
     2401 gtataacttt cgataaactt tcggttttag actccttagg cgttattcaa caaggcattg
     2461 gttatagttt aagatacaaa ttataaatac tctcaagtgt atattcagta tgggattgcg
     2521 caatgattgc ctaataaaat ttctgaaata tttctgtatc gcataatttt ttatatcaga
     2581 taaactgtac tggatttctt aaaaaattgc agtataattg ccgcaattat cccaccgttt
     2641 attttttgag tagtttctca tgatgcagca tacttctgtg tggtaccgac gctcggtcag
     2701 tccgtttgtt cttgtggcga gtgttgccgt tttcttgacc gcgaccgcca atcttacctt
     2761 ttttgataaa atcagccaaa cctatcccat cgcggacaat ctcggctttg tgctgacgat
     2821 cgctgtcgtg ctctttggcg cgatgctact gatcaccacg ctgttatcat cgtatcgcta
     2881 tgtgctaaag cctgtgttga ttttgctatt aatcatgggc gcggtgacca gttattttac
     2941 tgacacttat ggcacggtct atgatacgac catgctccaa aatgccctac agaccgacca
     3001 agccgagacc aaggatctat taaacgcagc gtttatcatg cgtatcattg gtttgggtgt
     3061 gctaccaagt ttgcttgtgg cttttgttaa ggtggattat ccgacttggg gcaagggttt
     3121 gatgcgccga ttgggcttga tcgtggcaag tcttgcgctg attttactgc ctgtggtggc
     3181 gttcagcagt cattatgcca gtttctttcg cgtgcataag ccgctgcgta gctatgtcaa
     3241 tccgatcatg ccaatctact cggtgggtaa gcttgccagt attgagtata aaaaagccag
     3301 tgcgccaaaa gataccattt atcacgccaa agacgcggta caagcaacca agcctgatat
     3361 gcgtaagcca cgcctagtgg tgttcgtcgt cggtgagacg gcacgcgccg atcatgtcag
     3421 cttcaatggc tatgagcgcg atactttccc acagcttgcc aagatcgatg gcgtgaccaa
     3481 ttttagcaat gtcacatcgt gcggcacatc gacggcgtat tctgtgccgt gtatgttcag
     3541 ctatctgggc gcggatgagt atgatgtcga taccgccaaa taccaagaaa atgtgctgga
     3601 tacgctggat cgcttgggcg taagtatctt gtggcgtgat aataattcgg actcaaaagg
     3661 cgtgatggat aagctgccaa aagcgcaatt tgccgattat aaatccgcga ccaacaacgc
     3721 catctgcaac accaatcctt ataacgaatg ccgcgatgtc ggtatgctcg ttggcttaga
     3781 tgactttgtc gctgccaata acggcaaaga tatgctgatc atgctgcacc aaatgggcaa
     3841 tcacgggcct gcgtatttta agcgatatga tgaaaagttt gccaaattca cgccagtgtg
     3901 tgaaggtaat gagcttgcca agtgcgaaca tcagtccttg atcaatgctt atgacaatgc
     3961 cttgcttgcc accgatgatt tcatcgctca aagtatccag tggctgcaga cgcacagcaa
     4021 tgcctatgat gtctcaatgc tgtatgtcag cgatcatggc gaaagtctgg gtgagaacgg
     4081 tgtctatcta catggtatgc caaatgcctt tgcaccaaaa gaacagcgca gtgtgcctgc
     4141 atttttctgg acggataagc aaactggcat cacgccaatg gcaaccgata ccgtcctgac
     4201 ccatgacgcg atcacgccga cattattaaa gctgtttgat gtcaccgcgg acaaagtcaa
     4261 agaccgcacc gcattcatcc gctgatttct ccctgtattt tttccaaacc caccgcacac
     4321 tccattcgta ttatgggcgg tggggtgggg tttgttatgc cgtatttatc aaataaacgc
     4381 ctacttgctg agatgagtat cgctcttgtc atggcgatcg ttgccacgct gacccttgag
     4441 cacagtcaga ttgatctgat ggtcgctgat tggttttatc tgggtatggg gcattggatg
     4501 gttgccaagc aagctttttt gccagatttg ctactgtatt ctggactaaa aaagctgctg
     4561 atggcgatgc tgatctactt gctggttgcg accatttgcc gtgcttatca tgagaaaaag
     4621 ggcaatgcta tcactgccaa gtggcttgtc ccagtgacaa aatttcgcgt gcgtgagctt
     4681 gcgtatctgg tgctgacttt gatcctagtg ccgacagttg tcgcgtcatt gaaggcatat
     4741 actcatgtgg tctgccctgt gcatttgacg atttttgatg gtacgctgcc gtatttgccg
     4801 atgcttgata gtatgcgtaa caccattcct gataagtgct ttcctgcggc gcatgccagt
     4861 agcggatttg cgctgtttgc ctttgcgttt gcgccaagtt tgcgccgccg tcgtggtgcg
     4921 atcatcatcg tggtgatggc attgggctgg gcgatgggct gctataagat gattattggc
     4981 gatcattttt tgagccatac ggtggtgtcg atgatgcttg cgtgggcgat gtcggcaggg
     5041 cttgcgtggg tgttttttaa gaagggtgaa caagtttaat cggctttaaa agtaaaaacg
     5101 gtcatatcgg ttattacgga acggtttttt cagttgagtt tcaaagcgat gattcatcaa
     5161 cctggaaact accaatagga acggctttac ttacgcctat cggtcaagaa ttgatgaaaa
     5221 tatgcggttc cactccagat attgcgtatt taaataaatt cctcaacaaa ataaatgttg
     5281 aaggaagcca ggtaaaacta tcaattatta atatgtaaag aggtcgagtt acctgtctga
     5341 gttccactaa ccataattaa aagcgaccgc aaaaactgcc cagtgaaaac tgtgttgcca
     5401 gtaatcacaa atgcagcaag tactaataaa gagcctgtta agcccgcaaa tatgtgggct
     5461 tatttttaat tactcttcat catcccgcag gaactgacaa aaatcttcgc tccccatcat
     5521 atcgacagtg tttttaaatt cgaaaaaaga aattcctgtc atagcacaca cgcgctggat
     5581 aatcagtttt ttcttttgct gtctgacggt tgcggtttca ccaaactgga taacagccag
     5641 gactgtttca tcatatgatt tcaggacaaa atcacaggtt atattctgca cttcacggat
     5701 taaatctttt acagcttcct cgctaccatg aggacgaaca aattcaatga gccgtatttt
     5761 gggaaaaacg gaataatcag gtataatatt acgtgaaaga atgcctaaaa actctgattc
     5821 attatttgag agtaagggta ctttagtaaa acaatcaacc tccccaatgg ctgtaaaagc
     5881 attggttgtc aaagctggat tgttcttaat aaaatcagag aatacatttt cagctgatgg
     5941 caaaacaaca gcagaattat gttgttcatc tcgtttttta ttacggttaa atatcatttc
     6001 atattccttt taaaagagcc tgcggcaggc acaggcaaaa acatcgtttt tggacaatct
     6061 gattcaaatg ttaacatttg acatcggatt gtctatcctg cattgttctt tactgtctgt
     6121 aagatcggcg ctcattcttc cggcgttctc ccgataccaa aaagacggaa cgcaaaacgg
     6181 ctttcgatcc accattcaaa aatctgctct gcgcaaccac aaacagttaa ggcgatccat
     6241 gcagcaagaa caacgtaact actgacatct gcacccatgt accaggcgat agttaaaccc
     6301 atcactgcga taccgcctaa aaactgcaac aagaaacgaa atcccgcaag aatccccaat
     6361 ggaataacaa caaccagata aagggaagca gccagaaggt atctgacagg tgtcagcaga
     6421 taaaagaacc tggatatttt ctttttgtca gaataaacgg catcatttgc aacatcggtt
     6481 gaatgatcat tcatgctgtc cctgattctt tgaaaatcta cgatattatc cttcatattg
     6541 ttatgacctc aattttcagg tgggtacatt ttatcaaaca tattagtaaa ctctgagtct
     6601 tccctggtat cgccctcata accaattgta taagagaact ttaatttata aacttttctc
     6661 ccaatttttt ctgaaactac gaaacttaag ttctttactt ccgtatgttt catgatttct
     6721 tttactgatt tattaagaac atctctttta aaagcaggat aatctggata cttataatcc
     6781 ttcacccccc ccgcccctat tgtataaaga ttcaattcat ctttaagttc atctattgtc
     6841 atttcaaaac tattcgcgcg agaattatta ctatatattt tacgaatgag ttgatatagt
     6901 gatgcagcat taacacttgt cagtcgaaca acagataata aaacttgcgt tgtataacgt
     6961 ttttcttcac caataagttt acataaaaat cttttcgctt ctttcgtaaa cttgatcccc
     7021 actctacctt cattcctgta ataagtagaa aaaacagtca gactcaaatt cattgaatca
     7081 ggtacattac ttttagtgaa cttaaatccc aaatcactac tcagttctaa tatttcatca
     7141 tgtttcagct taagaagcgt cgtatctaat atattagagc catccctcaa agcaagataa
     7201 gccgcatcag gcttaacctg aacccattta atgtaatcgg caactgtcac ataaaatatg
     7261 tgatcatcat cgaattcatt tttagagttt atctggcaca tggctaaaaa taagactttt
     7321 ttagcagcca taggcaatgt tgacagcgtt gaattcaatt catttcgatg tctcactttt
     7381 gttaagcgag aaagggaagt cttatttgtc gtcattctca ttgttccaac actcttgata
     7441 atcagacttt aacaccagaa acatgatatt acaacacaat caatatcatg tttaaccgag
     7501 tatcttctca tgtttcatat ctgtgattta tctcacaaag gtaatccatg ctcaaaatcc
     7561 acactggatt caaggcagat taacaacaga ttcaaaatag attcaccaga aagaacaacg
     7621 aattagtatt cttatgacac attttgtgga tatcaccgag gaagctctca tgtttaaccg
     7681 aggaagctct catgtttaac cgaggaagct ctcatgttta accgaggaag ctctcatgtt
     7741 taaccgagga agttatcatg tttaaccgag gaagctctca tgtttagaca aaaaacacgc
     7801 ttaaaatcaa taaattagag ctactctaag tttttaagtt tttaagaaaa acaagaaaag
     7861 ataaggcttt taatgcgtgc gaatattcag gaactgtgga taactcaaca aaaccagcaa
     7921 cgatgatggt tccttaccat aaatctgtcc cctgatgatt attacccgca agctaaaaca
     7981 aacatcatca atcattaaaa ctcataactt tcaataacat aaaatcatca aaaaaatatt
     8041 tgatgcctta tgtatctcaa ttttcaagac cataaatttg tccctagtta actttaactt
     8101 attgaaataa atataatatt cgtgcgtact gatcctaatg tcacatcgca aaaattccgc
     8161 ttcctcgctc actcagtcgc tacgctcctg ccgttcggct gcggcgagcg gtatctcctt
     8221 tctcaaaacc agagagattc agatcatatg atccgttatt tgatcctttt tggatcttcc
     8281 actaagaagc cctgaatcgc gctctgccgc ttcgttcgat acaaccatga ggaaaaccat
     8341 gcgaaatgaa aaaaatcgct taaaatgcga ttcagagcgt tttagggagg ttctgttagt
     8401 tctcctgcat tgcgcccgta gttcgctaac gctcacacgc gcagtacagc gccccgccat
     8461 gctgcaatgc accagggcgg ggtaaactcg cttgcgctca cgctaactgc ggcattctgc
     8521 ggcgcaaatc agcataatca tggcctgctg gtcagttggg ctttgcaggg tgaagggatg
     8581 gcctgtgcac cctggtacgt tcgtcagcgg ctgcgccgcg cttcggggcg atgtggctcc
     8641 tgtgctgccc tggtattttc gttcgcccct tccatccgct tacgcggatt aaaaaacgtg
     8701 ataacaatca cttatcgagt aaaaaaacgg gagaaaactt gcaaatacga cttaaaggtc
     8761 gtattattgt ttcatcgaaa ggggaatggc ccctctcaca atagcccagc agggcaggag
     8821 attaaaaaaa tgaatatcca ggaagcatta aacgtttttg gattatccgg cgaattaact
     8881 gaaaaagata tcaaagcagc atacagaaaa gccgctttaa aatatcatcc agatcgtaac
     8941 ccgttagggg ctgaactgat gaaagcggta aatgcagctt ttgatgtatt gatggcaaat
     9001 attgataaaa taaatcagtt ccagagcact gatgaacatg cacgatataa ttacggtgat
     9061 gacctggaaa aagtattaaa cgttctttct ggtttatctg gtctggtatt tgaagtgata
     9121 ggtaactggg tctggattag tggagaaacc attacacata aggaaacttt aaaagaaatc
     9181 gggtgtaaat gggcggcaaa gaaaaaacaa tggttttatc gtccagacga acataaaagt
     9241 tactggaatc gtgaagaaca cacgatagaa gaaatccgcg caaaatacgg tacaaccgga
     9301 cagcgcaggg cgacagggtg gcaacgcgtg gaaaccagag cgtaaccaga acgggggcga
     9361 gaagcccccg atattatagc cagcccgtct tttggggcat cgaacccaaa aagacgggaa
     9421 cagcagccac gtagcaaaaa gggaaacaga tgaactcttt ttttgaacaa tatcaccctg
     9481 tctttgaagt tgtctgccgt atcctgggga acggctggcg cgtgaacaaa cttgatgatt
     9541 gttcgtctcg tataaaactg acgtcaccgc agtttaaaaa ttactctgtg catattcgga
     9601 tggaaaaaga ccgcttttct gtggtgggga gtgtggatag tcgttcctgg cgtagtccgc
     9661 atcatgtttg cacgttatcc agaaaacgga atcccgttga tattgcagcc gatatcgagc
     9721 gaaaaattct ggtaaatgct tcgcaggagg tgttacaggc gattgagtat gagaaacacc
     9781 aggtggaaaa aaaggacgaa attctgatcc tgaaaggtat gttatcgcaa cttgtccagc
     9841 ttgaaagctg gtatggggca ttgacaggct ttaaagctga aaacggattg aacggtaaag
     9901 tcaccgaaca gggcgacagt tatgatttac agattcgggg cttgagtata gatcaacttg
     9961 ttaaaattac agggtatttg aaacagttat gagaaaaaat aaaggtgatg tgacttattt
    10021 tcttgaaaaa gaaggtgaca attacaggct tacaaaaagg ataaaagcca gaacaaatgt
    10081 taaaatcggg aacaaaacta cgaaaattac attgtatgat gctgtactga atgagaatga
    10141 attacaacat attgatttta cgtgtgccgg attaagggaa gacgatgaga cgccagttaa
    10201 gaatttaatt aaggagttta tgttaaatga aacccgataa tacacctgaa aatgtaaaaa
    10261 aattacgttt aaaagcagga ttaacacaaa aggaatgcag tcatatttat ggtgttgggc
    10321 taagaacctg gcagaaaaaa gaggaagtta atactcaaaa cagtcaaagt ctgtcactgg
    10381 ttgagtttga gtttcttttg ttacttgcag gagaacatcc tgaatacgtg ctctgcaaaa
    10441 gggagtcaaa ataaaatgac ctgtgatggt tttatattag tacctggagt tgcgctcgta
    10501 gctcgctgac gctcacacgc gcagtacagc gccccgccat gctgcaatgc accagggcgg
    10561 ggtaaactcg cttgcgctcg cgctaactgc ggcattctgc ggcgcaaatc agcataatca
    10621 tggcctgctg gtcagttggg ctttgcaggg tgaagggatg gcctgtgcac cctggtacgt
    10681 tcgtcagcgg ctgcgccgcg cttcggggcg atgtggctcc tgtgctgccc tggtattttc
    10741 gttcgcccct tccatccgct tacgcggatt aaaaaacgtg ataacaatca cttatcgagt
    10801 aaaaaaacgg gagaaaactt gcaaatacga cttaaaggtc gtattattgt ttcatcgaaa
    10861 ggggaatggc ccctctcaca atagcccagc agggcaggag atttaaaatg aaatgctcat
    10921 ctgtttttac ttcaaccact aatcatgttt tcacattcga acgagtaacg ctttgcacta
    10981 ttatccttat gcataaggat actggacaac agtatgttgt catatttact gataacaata
    11041 aaattcgtga ctataaagcc ggaattgttc ctcagtttgg cgaacttaag cagagtgatg
    11101 ttgatttagt ccttttctac agggacgaat atgaaaaata ttttgattca ttaaaagatg
    11161 gcgatgaatg cttgagtttt aaagatttta ttgagtgtct ttgttaagca ttatgaatgc
    11221 aggggcatcc ctgcattttc tggaaatttc aatattgatt ctgaaattgt ctattaacat
    11281 caatgcaagg gaaattcatg aaaacgttaa aacaagccgc aatgcagttt gcatctgatc
    11341 tgcgtaataa tcgctgtttt aaagctgctt ataatgatgc tctgttagag cttgatcggg
    11401 aagatttaaa cgccattgta caatgcacat ttctttttga gccaaaacga acattagccg
    11461 aacttgataa attaattaaa aagatagaag tagaagaaga ttatgagttt tgtgtatccc
    11521 ttgataaagg taaaggatta cacgaagtca gaacatttaa aagtgagcgt gaggcgttgg
    11581 atgcttatcg cattttgact ctttacggat acacagtaac tatagaaaag aaggagggta
    11641 atgactgact attcaaggtg tgatcaggtt gttccaccaa cctgatcacg ttatcgacaa
    11701 tcaaaactga atgaggtttt taaatgtcaa cgcggcagat cgtacagctt gctgattccc
    11761 ttgtcaaagg ctgggaaacc agaattccgg caatgaaatt tagcgaatgg gatcaattcc
    11821 ggtggtggtt aaactacttg caagggtatg aaatgtttta aataaaaaaa ggggctttct
    11881 gcccctttgt attttttgac aaaaatgcca tgtgtaacta taattagttg catatctata
    11941 ggggatggat ttatggggaa aacagataag ctactggcaa agtttttaaa cagtaaaaaa
    12001 acgtttgaat gggatgaact ggtcgttttg ttttcctctt tgggatatgt caaaaaggaa
    12061 atgcaggggt caagagtgcg gtttttcaat gctgaaatca atcacaccat attaatgcat
    12121 cgtccacatc cggaaagtta tatcaaaggt ggtacgctga aagcaattaa acagaatctg
    12181 aaagaggctg gattattatg aaacatttaa aatataaagg atatttaggc acggttgaac
    12241 ccgattttga aaataatgtc ctgtatggaa aactggcgtt tattcgggac ctggtaactt
    12301 atgaggcttc tacattagct gaactggaac aggagtttaa aacatctgtt gatctgtatt
    12361 tacagtcttg tgtggaggat ggaaaggaac ctgatacccc gtttaaaggc gtgtttaacg
    12421 tcaggcttga tccagaactg catcgtcggg tagcagaaat ggcgatggaa gaagatttat
    12481 cgttgaatgc ctttgttaat aaggctctgg aaaaagaagt cagcaatcat cgcgcagggg
    12541 cttaattgcc cttgttttta tgccctgtaa gggcatggga ggcgctttgc gccggaagcc
    12601 ccgcagcgca gcgaggactt aaaaagcgtt acggcttcag ccgtaacaat tcccagccct
    12661 gccaggcctg ttttttcttt cacaaatctt tcagctatac gtttgcctga tggtcgaaga
    12721 cgaaagaatc tttcagcaac gaaatctgga catatgcagc gacgatttta tagccaggca
    12781 cagtgccaca tatggtacaa tcaatgcagg atagacaatc cgatgtcaaa tgttaacatt
    12841 tgaatcagat tgtccaaaaa cgatgttttt gcctgtgcct gccgcaggct ctgttggggt
    12901 gacttatgaa gatgatagcg ttcaggctaa gtgatgatga gttaaaattt gcagaacata
    12961 acgctatttt aagtggattt accagtatca atgcttttgc taagcataac gtgcttaata
    13021 ttgaaaccaa gcctgttaac atcccagtta ataatgaacc agcaaagctg gtatcagtgc
    13081 gtctttaccc tcatgaaata gaacttgtta aacgtaatgc tgctcttcac ggcatgagca
    13141 tgagcagaga aattgctatt cgtgtgcgtc agtctttgtt aaagagtgaa gtctgtcttt
    13201 accctgatga agttaaggaa cttaaaaaac tatcaactgc tgtagatcgt gttggtcgca
    13261 acattcattt tatcataaaa ggtgaaagat tctgtacggt taacgatcca gatttcagaa
    13321 aagacgttat tgaagtgatt gaactgtgta agcaaattga ttcaaaactt gaaacgttaa
    13381 ccaagagtgt tgttaacaga tttgggtgat ttatgggtgt gtatgtagaa caggaatacc
    13441 gtattaaacg cgctaaaggg actgggcgtg atccgaaatc tcctaagtta tcgggaagac
    13501 atatacatgc cagtaaatcg gcttttaatc ataaagtcag acatggtgcg gacaagaaag
    13561 catatacctg gacacctaca aaagaagtca cttttaaaat cactggctca ggtaaaacag
    13621 ccgcaggcat caaaaatggt attgattaca ttacccgtaa tggtgaactt gaggcttact
    13681 gttatgatgg aaaaggttca gagcaaacag ggaaaggaga agactttaat agggagttta
    13741 cttcaacttt atctaaagga aatgattact ccaggacata tcgtggagaa aacatcgatc
    13801 atgtgaaaaa catggtcttt tcaccaccac cagaagctgg agtaagcagg gaagacgctc
    13861 tgaaagctac ggtagaattt cttaaagaga cttatccaaa ccatgctttc gttgccgtat
    13921 accatgatga taaagaagat catcctcatg tacatgttaa tattaaactt cgtgatgaag
    13981 aaacaggaag aagattaaga cttactaaat ctgaaaccag gaagttcaga aacggttttc
    14041 atagaaaact gaaaggtatg ggatatgatg ttaccgctac ctggaaaaaa gatcccgaac
    14101 gtaaacggga gattgagcga ttacaggctg aaaatccgaa aagactccgg aatgtttaca
    14161 aggtggtgga ttttggggag acttcatatc agaataaggc aggggaaaaa cgaacacctt
    14221 tcctcactta tgaaacttta aaaggtggaa aacaggttac gatttggggt aaagacctga
    14281 aaaatcattt cgagtctgaa aagctccagc caggaacgct tatcaaagta aaaaaactgg
    14341 ctcctacgct tgtacgtagt ccaatgttta atgatgatgg aactgttgca ggctacaggg
    14401 aaacgcatcg caataactgg cagattgaaa atattgccct tgagcggaac agagaacgtc
    14461 aggttcatga gcgtgaaacc aggcagccgg aagagagcga cgttaaaaag cagctatcca
    14521 gaaaacatga gcaaggtcac aatatcgggt ttgctcttga acacgggttt acaaaggatt
    14581 cagaagaaca taaaaaacta agaatacagc aagagaggaa ctggaaaggc ctgggatttt
    14641 aatttgattt tggcgcaggt gaacaaagcg gctactttgc tcacctgctg atcacaacaa
    14701 cctacgaggg aggttatcat gactgagcat gatgctatct gcatttctgg acttcatcaa
    14761 atttttagtg atgaagaaca tttgagtgag caacaaaaag acattattct gatgtacgct
    14821 tatggctata ccctgaatga aattgctgat ttcaaaggac taaagccttc aactgtcaga
    14881 aaatatctcg attctgtaag agctgaactg ggaggtgtct cgttggcagg gatcagaaca
    14941 ctggtactca ttcggactaa tgcccttctc gtgtcaagtc tttccaggat cagtgagagg
    15001 gggaatttat gaaaaaatat ctgctttttg ctttgccgtt cttcgttgtt ggttgtagtg
    15061 aagaagtaaa aagtgtggat tggtgggggc agcatcttac ggaagctaaa caaaagcagg
    15121 cagagtgtga gaagtctggc tctgattctc agaactgtaa gaatgttaaa caagcattgt
    15181 ttattcagtc tcagaaggat gctccagtcc ctacattcga ttaactaatg tttcaatcat
    15241 actagagcgc ccatatgggc gctttttttt gcatgatcac acgaatcata atatgaccat
    15301 tttgtcaatg ttatttgatc gtattgttga gcattgttat tttgcgaatc atgttcgcat
    15361 ctaaaacaaa tccaaaacac acttatagtg gattgattaa atgaaggaga aatcagataa
    15421 gtcatcggaa aaaggggttt atgtcacgat aaaactcgat cccaggttga atgcatttat
    15481 cgaagagaaa acgaaaacat ctttgcttag taagaggaag caaattattt acttattaat
    15541 gcagagtatg cgtattgatg gatatatgga gcgttagcta tggcgtcttt attcccagcc
    15601 ttgttgctgt gtgcggcgtc agttcatccg gatacgataa atgatattgc acgggtcgag
    15661 agtggcttca atccttatgc tgtggctgaa attattcctg aaagggaacg cggtagctat
    15721 ggcaaaagtg ttatcagtca tatgcccaaa agtaaggagg aagctctaac agttatcaga
    15781 gaaattgaaa acagaaaaag acgttattct gtcggattga tgcagattac cagcagtaat
    15841 ttttcttttt attccacgtc cgcagaaaaa ctccttgatt cgtgtgaaaa cttgtctgtt
    15901 ttcgaaaaga ttatcgtgga ttgttataaa cgagggagga gcctggagaa tgcgcttagt
    15961 tgttactata caggaaattt tagtaacggt aagcgaaaag aaaaagaatt taataataca
    16021 agttatgttg agcgaattgg ttatacaggg aacgaaaaaa aatatgttgt accaggtaca
    16081 agaagcaacg ggggagaaca gcgaaaaaat cgttcacaca atgcgtcagt tatctggccc
    16141 gaaaccattc tgaaatctgc ttttgttgat aactcacatc caacaaaagt tattaattaa
    16201 caggacttat aaaatgtcat ttaagaaatc agtatgtaat cgtatcagtt catctgtcac
    16261 tgctgtaatg cagcgtaaaa ataaggtagt tatggcagtc gccggggcaa cagttgcatc
    16321 accggccttt gcggatggat tttcaaaagc ggaaacactg ctggaaaagg ttaaaacagg
    16381 gcttagtggc ctttcactgg tcacggtgac tattgcctgt ctgtgggtcg gctacaaggt
    16441 tctgtttggt ggaagcacga ttcgtgaatg ctcacctatc atcatcgggg caattgttat
    16501 cgccagtgcg gctgaagttg catcaatgat ggttaactaa taaagcgggg acgatagtat
    16561 gtctacactt tataaagcga tgacacgccc tgctatgtat gtgggtgttc ctgtcgtccc
    16621 tcttactgtg gttgcagggg cgttatttct ggcgggggtt tatatcagca agctgatctg
    16681 gctggctatt cctgtcgcgg tttttgttct cagaatgatc actaaacagg acgaccacat
    16741 ttttaacctt tactttctta agctgaaaat gctcggaaac tcagtgtgta atcgtttttt
    16801 tggcgcacga gcgttcctct ccggacaata tgaagctgtt gaaattgatg agtttgttaa
    16861 tgccatgaaa ctgaatgagc gaatcactac tggtaaatat ataccctatt caagccatgt
    16921 tgataaaaat attgtcaaaa caaagaacgg tgattatgta gcgacatggc aactgatggg
    16981 gattaacttt gagtcaatct ctgcggagat gttagaaact attgactcac aggttgccac
    17041 tcttgttcgt tcattttccg gactgcccgt ttcattttat aaccattcat gcagggcgag
    17101 tttttatgat gcctttacca caaaatcagg taataaatat gctgatataa tatcagattg
    17161 ttactatggt tcgatgaaga aaaataaatt caaggggaac actctttact ttacgctgat
    17221 ctacagacca gatgggcgtg tagaaaaact ggaaaaacgc aaaaaaagta ttaaagaaaa
    17281 gaaagatgat attaacattc atgtgaagag aatgaatgaa atgattaata cattctctgg
    17341 cgctcttgat aaatttacct gtaaacttct tggtatgtat gaggagaacg gaaaggtatt
    17401 ttcttctcaa ctgtcattct ataattatct tctgacaggg aagttgcaaa aaatcagagt
    17461 cactgattct ccggtgtata acgttcttgg tggagtggat gtatttttta atcatgatac
    17521 cggacagata tgccgcattg atggaaataa attctttcgt tctattgaaa ttaaagattt
    17581 ctgtagtgag actgcatccg gtgtttttga tgtgctccag tattctgatg ctgactatat
    17641 tattacgcat tcatatacat ccatgagcaa atcagaagca ttgagtacca ttaaacgtgc
    17701 ggaaaaacag ttaaagagta cagaagatga tgcggttact caattgcagg aactggaaaa
    17761 ggcaaaaaat gatattgtgt ccggtgatat atcctttggt tactaccatt tcacgctaat
    17821 ggtaatggcg gacagtatca gggagcttga cgaatctgta agtaaaatta cggcagactt
    17881 tactgacctg ggcattattc cggcattgtc aaccatgtca ttgccagcgg catattttgc
    17941 ccaacttccg gcggtttttc atttaaggcc gcgtttatct ccggtatcga atgtaaattt
    18001 tgttgaactg gcatcattcc ataatttcta ccaggggaaa cgcgataaaa actgctggac
    18061 agaagcggtt gctattctca aaacaccaag caaacaggcg tattatctga atttacataa
    18121 ctcagtactg tttaaagatg aaacaggtga aaagaatctt gctaatacta aagttatagg
    18181 aacggcgggt tcaggtaaga caatgtttct gtcatatctt gcctgttcat tacagaaata
    18241 caataacccc gaaacttttg ctgattctgc aaaaaataag aagttaacgt gtgtctttct
    18301 tgataaggac agaggggctg aattatgtat ccgaatgctt ggtggggaat attacaccgt
    18361 aaaaagcggt gagccgacag gatggaatcc ttttgctctg gaggcaacaa aacgtaaccg
    18421 tatttttgtc aagcaactga tggagattct gtgtaccaga aatggtgaaa gattatcgac
    18481 aagagaaaga ttgcttatta gcgaaagtgt tgatgctgtg atggattttc ctcctggcga
    18541 aatgcgggaa tatggcatta ccagaatgct tgagcatctt atgcagagag atgatcggga
    18601 tgagcaggag aacgggatta tccttcgttt gtcccaatgg gctaatggtc aggctcatgg
    18661 ctgggttttt gataatgcta aagacacatt caatattcag catgtcaata attttggcat
    18721 tgatggcacc gagtttcttg atgatccgat ggtatgtgca ccaattactt tctatctgct
    18781 gtatcgcatt acgcagcttc tggacggtcg gcgtcttgtt atcttcctgg atgagttctg
    18841 gaaatggcta caggatgaag cattcagcga ctttgtctat aacaagctga aaacaattcg
    18901 taagctgaac gggctggtta ttccggcaac tcagtctccg gatgaaatac tgaagaataa
    18961 gatttcacgg gctgtggttg aagtatgcag caccagtatt tatctggcta accctgatgc
    19021 cgattacaat gattatgtgg aagggcttaa gctgacacca gaagaattta atattgttaa
    19081 aaatcttgat ccaatgtcga gacagttcct gattaaaaaa agcagtctta aaaaggggga
    19141 tggtaaatca ttttcagcac ttgcgacact cgatttatcg ggacttggtg gatatctgaa
    19201 aatattgtct gccagtgcag ataatctgga aatttttgaa agtatttatc atgaaggaat
    19261 ggagcctgat gactgggttc ctgaatatct cgaacgggca atctgacagg tgaatttatg
    19321 aaacgtgtaa aaacattgat gctgatatca ttattgacgg cttcatttta tagtagcgca
    19381 ggtattccgg ttgctattga tgccagtccg gaatgggctg ttgaagccac aagatggaca
    19441 gaacgactca aacaatggtc tgaaacagcg cggcattatc aaagtcagat tcaggcatat
    19501 aaagaccagt tagccacggc aacaggtata aggaatattg cggcattcac aaatgaacta
    19561 agtaacctac aatcagagct ggcaaacatt tataagcagg ggaacagtta tatttctgat
    19621 tttaccagta atcctgaagg tgcattaagt agtcaggcga aaaatctgtt cagcaagtat
    19681 ggtgctttta atatgtgttc tacaggcatt gaaagttctg ataacctttg taaggccaga
    19741 atagtttcaa cggccgctag cattgaacag ggaaatgaga taaataagca gctatcaagt
    19801 gcaatgtctc agattcagag tttaagtaac cgaattgagt cttcaaagga tatgaaagaa
    19861 tcccaggatt tggctaacgc attgcaggcg cagtcgttaa aaatacaggc aattaaaatg
    19921 cagtatgatg tatgggatag taaaaataaa gcagatcatg agatgttggt cacacaagaa
    19981 caagaggcct ttattaaaca gcagaaagaa gctgaaccat taacttttga ttaaaaggtg
    20041 gatatatggc tcaggggttt ttcgttaaat ataattctac cgttatggat tcggtagata
    20101 aaatcagctc cagttatcaa acacaatttg ctaatgatat tatgtcgtta gcaacggtat
    20161 cggttacgtt atatgtacta tggaaaggat atcaaatact tgccagtaaa actcagacgc
    20221 ccttgcagga tttagtatgg gatctatcaa aatttgcaat cataatcatg tttataacta
    20281 atgcggatgg gtatttaact gcggctactg atgcgttgca aggcatgaaa gatggttttt
    20341 ctggaggggt gagtgtctgg caaactttag ataacttatg gaaaagtacg caaaatcttg
    20401 gtgcggaaat atactcttta gataaatcaa catatgttaa agatcaaggt gtagttggtc
    20461 agtttttaat atggactggc tcgcttattt taatggctgt tagtgtggtt gtttttttaa
    20521 cagcagatgt aacgatgaaa ctcctgatta ttacagcacc gatatttatc ttttgtctca
    20581 tgtttggttt tattcggagc atgtttaata attggttaca atcgttattc tccagtattc
    20641 tgacagttct atttgccagt ttagtgattc ggatagcaat ggattttcaa gggatgatcc
    20701 tttcccatgc tagactggcc ccctgaatct ccagacaacc aatatcactt aaataagtga
    20761 tagtcttaat actagttttt agactagtca ttggagagca gatgattgat gtcttaggac
    20821 cggagaaacg cagacggcgt actacacagg aaaagatcgc tattgttcag cagagttttg
    20881 aaccgggaat gacggtctcc cttgttgccc ggcaacacgg tgtggcagcc agccagttat
    20941 ttctctggcg caagcaatac caggagggaa gtcttactgc tgtggccgca ggagagcagg
    21001 tcgttcctgc ctctgaactt gctgctgcca tgaagcagat taaagaactc cagcgcctgc
    21061 tcggcaaaaa aacgatggaa aatgaactcc ttaaagaagc cgttgaatac gggcgtgcaa
    21121 aaaagtggat agcgcacgcg cccttattgc ccggggatgg ggagtaagct tagtcagccg
    21181 ttgtctccgg gtgtcgcgtg cgcagttgca cgtaattctc agacgaaccg atgactggaa
    21241 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
    21301 tatcggagag ctgcccacgt atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
    21361 cgaacttgat ggtatgcctg cgatcaatgc caaacgtgtt taccggatca tgcgccagaa
    21421 tgcgctgttg cttgagcgaa aaaccgctgt accgccatcg aaacgggcac atactggcaa
    21481 agtggccgtg aaagaaagca atcaacgatg gtgctctgac gggttcgagt tccgctgtga
    21541 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
    21601 ctgggcagtc actacgggcg gcttcgacag tgaaacagta caggacgtaa tgctgggagc
    21661 ggtggaacgc cgcttcggca acgagcttcc ggcgtctcca gtagagtggc tgacggataa
    21721 tggttcatgc taccgggcta atgaaacacg gcagtttgcc cggatgttgg ggcttgaacc
    21781 gaagaacacg gcggtgcgga gtccggagag taacggcata gcagagagct tcgtgaaaac
    21841 gataaagcgt gactacatca gtatcatgcc taaaccagac gggttaacgg cagcaaagaa
    21901 ccttgcagag gcgttcgagc attataacga atggcatccg catagtgcac tgggttatcg
    21961 ctcgccacgg gaatatctgc ggcagcaggc cagtaatggg ttaagtgata acaggtgtct
    22021 ggaaatatag gggcaaatcc acatgctatt cgtgctgcac aaacagggaa tgtaaatctt
    22081 gtatctacag gtgctatggg ctttatggct gggtttttgg gggctttatt ggttcttata
    22141 gctaaaggtt ttgctgtcca gttagcaggt gcaggtgttg aaggtgctgt tcagggcgca
    22201 gccatgatgg ggctgggtgc tgctggtatg gctactggta agtctctgat gcttggcggt
    22261 cgtgctggtt taggttttgg cttagggatg gccggaagaa cgggcatgaa ttctttatca
    22321 gggaaagcag ggaatctgat ggggcgtggt gcgcgaacag ccgctgaatg ggcgggtgaa
    22381 aagggatatc aggcgctggc tccgacaggt gctttgggga tgaaggcgag aagacttgca
    22441 tccctggaaa aagcacgagc gaggaacgcc gcatgaaaat aatgaaacgt tttttttatg
    22501 tgatgtccct gaactggata ctggtgttcg ttgataaaca tatcacctcc ggatggagca
    22561 ggaagagaaa gctaataacc cagcttgtta ttttaatcac gggaattacc ttatttaagg
    22621 tcttaggtgg aatcagtcag agctggttgt gacatgcttc attattttgt catgatactt
    22681 tattttgtcc taatccttct tttaggagtg tttacctgct tcgcaggaat aagacttggt
    22741 ttagggcttg cggggaaatg gagaaagggt atggatcttc gctttttgct tcttattggt
    22801 gggattcttt ttcttcatct tggcctttat ctcttaaagg tgttcgtgaa cgcttaataa
    22861 ggtgaattta tgcgatgtgt gattcttttg tttttcctgt ttcagatttc cggatgtacc
    22921 acaaggaata accttccttc tgatgtttcc ggcgaactgg aacccattaa ccacagccag
    22981 gtaatcagtt atgagtgaaa aaatcgaaaa gaagattgag acagctaaat ccttcgaaag
    23041 acaactctat gcagaaaatg aacggtctaa gaaaaatgca tggcgtgttg cagtggcagc
    23101 atcattactg tccttatgtc ttggggctgc aatttgtgtt cttgtgccat taaaagaaaa
    23161 agagcttgct attgtggctg ttggcgaaaa aacaggccgt acagaactga ttactcaggt
    23221 taaacaggaa aagatcctgc aatctgaagc acttgggcgg tattttgtaa acacgtatat
    23281 tactttacgt gaaggttata attatccgtc tcttcagtat gattatgaga ctgtacagct
    23341 atatagctcg aatacagtaa aggacgatta tcttcgtctg tataacagcg atatggctcc
    23401 tgataaaatt tatcataata acggttctta cgttgcggtt gaggtcatct ccaatattat
    23461 ttctgacgca accgcgccag ataaactggc ctctgtgcgc tttaaaaaaa cgacgagaaa
    23521 cttcacgacc ggacaggttt cagtttcata ctggacggct cgtgtgacat atcgttttga
    23581 accagaaaag tcggttaaaa gttccagtcg tgagcttaat cctctcggtt ttactgtaac
    23641 cagttaccag acagatcggg aagtcagggg ggaatgatga agaaattatt tattgctaca
    23701 tgtttattgc ttcctgggct tgtgtattca gcagcgactc cgttaccttc tggatttgat
    23761 gcaaggatgc agactgtgag ttataacggt gcgaatacga ccgttatccg gtcaaagaca
    23821 ggatttctca catcagttgt ttttgatgaa ggtgaggctg ttatcagtgc aaaggctggt
    23881 tttcctgccg gatgggaaat cacaaccgat gataatgttg tttacatcaa tccgcgtccc
    23941 gttgttcagg aacaggaagg cgatgaaggc gaaaaactga aaaaggtttt ccagccaaca
    24001 gaaaaggaat gggatacgaa tctttttgtc agaacaacaa agcggattta cagtctggat
    24061 ctaatccttc tgtcagaaga aaaacaagcg caacctgctt atgttgtaca attccgctat
    24121 ccatcagaaa tagcgaaaaa gaatgcagaa gaagtaaggc tggcaaaaga aaaacaggaa
    24181 aaactcagac agaagaaact gatttctgaa agttttgaaa aagccgatgc gcctaaaaac
    24241 tggaattatt tcatgcgagt caatgaaaaa tatgacagtc gtcgtatcgc ccctgatttt
    24301 gcttatgata atggcatatt taccttttta ggttttaact cagggaaagt attcccagct
    24361 ccatttgctg taagagatgg tcaggaacag acacttgctt ttaatgtgga aacaaaaggc
    24421 aagtataaaa ttatggttat ccacaacgtt aatgacaaat ttgttttacg ctatggaaac
    24481 agcgttgttg gtgttgtgaa taaatctttc ggaaaagtat taaccgacca gaggaataca
    24541 tcttctcctg cggtagaacg tgttgaggtg aataatgact gatatgaaaa aaacagatga
    24601 tttagttgat gatgcgaatc agtcaaagct ggaaagtaaa cccgttcatg atatcggtga
    24661 tatcaggaac aaaaataatc gtatgcgttc tggctttctg tttggtctga tggctgtatt
    24721 agttattggt atttttgcgc tgaaaacata taaaaattat tttgcagatg accagcagaa
    24781 agcatcagaa tcaacaggcg ataccagcat ttctcaggta agtaaaatcc gtactggtct
    24841 tggtcagaat tttgaccctg ttgagaacaa agcaattatc aatccaggcg ctactggttc
    24901 tggtaatact gtttctgacg gtcataaaga agtgccacag gaggggttca ggaaatattt
    24961 gtcaatacct gttgctggtc agggagggag tcagactggc gcgagtggca gcagcaggac
    25021 atcgcagaca tcagagccac aggaagaaag gaaagaatca aaagaaaatg tgcctggtaa
    25081 atcaggaatg aaagtaactg cgattaatct cgatcctgat ctctatattg aggaaaacag
    25141 gcttataccg tgtgctttaa ccacacgctt tgtttctgat gttgcaggtc gtatcagttg
    25201 tgtttttaca gaagatgtat ggagtgctaa tcaccacaca aaattactgg aacagggaac
    25261 taaggctttt ggtcgttacc agaccggaac gctgaatcat ggacagggca gaatgttcgt
    25321 tatgtgggag cagttgcgga cgcctgataa taaacgaatc gatatggtaa atacagcagc
    25381 agctggtccc ctgggagaag cgggcattga tggatggatc gattctcact tctgggagcg
    25441 ttttggtggt tctcttatgc tcagtatggt tcaggatgta gcggcggcgg cggcggataa
    25501 tgcacctggt aaagatcgta atgtggatta taccgaaaac tcacgacagg caatggctga
    25561 aatggctaaa gtcgcacttg agaattcgat aaatattcct ccaacaatgt ataaaaacca
    25621 gggtgacata atcactatta tggtcgggga agacattgat ttttctgata tctatgagtt
    25681 aaaggtaaaa tagtatgagt ggcgttattc ctgtcagcat tagcaataaa tcgctcgatt
    25741 tttataaaaa tcaagtattc cagtcttttc ttgatattga agggctaaca gaaattgctg
    25801 tcaaccgtcc tggtgaaatc tggacggaaa ttaatggtga ctggacattc cataataatg
    25861 acagtgtgac ctttgatttt tgtaaacgtt tttctcatac gctggcatca tttcggggag
    25921 atgaaatcgg cgagaccaag ccattacttt cagcaacact ggaatcagga gaacgtgtac
    25981 aggtcgtttt tcctcctgca tgtgagagaa atactatttc aatcactata cgtaaaccat
    26041 cgacacggca gataacacat aaagaatatg tggcgaacgg tttttatgat tatgtccagt
    26101 caggaaaaaa acacaggaca tacgatgatg agcttcttga tttattcaga gcgagaaata
    26161 tcgctgcttt tatggagctt gccgtagcag cagggaaaac cattgtattt gccggagcaa
    26221 cgggttccgg taaaacaact tatatgaaga gtcttatcga tttcatacca ttgaatacac
    26281 ggctgattac gattgaagat gcagaggaaa tcaaattttt tatccataaa aattatgtgc
    26341 atctttttta cccttcggaa tccggcagtg attccggctc tatcataaca gcagcgaaat
    26401 taatcaaatc ctgtttgagg atgaaacctg acagaatttt actggcggaa attaaaggtg
    26461 gcgatgcctg ggattttgtg aaagttgctg gttctggtca cagtggaagc atgacgtcca
    26521 ttcacgcggc ctcggcaaaa gatgccatca ttcagatggt gacaaaatgt tatcagaaca
    26581 gagaatgtca gaatcttcct tttgatgttc ttcgtaaaat cattatggac agtattgata
    26641 ttgttgttca tgtaggcagg gatggtgttg tcaggcatat gagcgacatc tattacaaag
    26701 gggctgaatg tgaaaacttt taataagtcg caatctgttt ttatattcgc tgttatgctt
    26761 gttgcggcat ggtttgcagg ttcatttatt ttctttgtgc tttacttcag ccagataaag
    26821 aatctgaaac cattgaaggc tgtgttcaga tccattgatg cgtacaggat ggatattttt
    26881 acagacagcc ttactttatc tgttgtgact acggatatca gagttttagc ctttgttgca
    26941 cttggagcag gatttgtcgt gtctcttatc atccctgttg cggcgttgat aaaactgaac
    27001 gagaagaaag aaaatatttt cggtgatgcg cgatttgcaa caattaatga tattcgtgag
    27061 tctaacagtt tcacgcttga cggtgacgaa aaagacggga ttatcgttgg tataaaagac
    27121 aagaaaatca tacgctatgt gggagcagca ttcagtgcta tgggagccgg aacaagggct
    27181 ggtaaaggtg ctggtatagt catcactaat cttatgaagt actggtggag tgttattatt
    27241 ctcgatccaa aaagggagtg ttttaatatc accagtctta tcaggaaggt gattttaggt
    27301 catgaggttt ataaatttga tccctttagt tcggtaacgc accgctttaa ccctttgtat
    27361 tatgtcagca tgggaacgag tgaaggtttt aatcagcttg aaaatctttc gttgattatt
    27421 tacccctata agacagatgg tgcggatgct ggtagctatc ttaataagac ggcgggtggt
    27481 gtttttaaat cttatgctgt cgctttatgg tttatgatta agaatgataa ggctggacta
    27541 aaaacgcttg atattgagcc tgtcttctca atgtctaaaa ttaatcagct ttttgagcgt
    27601 gcagaacccg aacatcttct ttcattcatg aatgatattc gttcagaatt aagggggaaa
    27661 gacaaaacgc ttgctgatat tggtgtcgct ggccttaagg cattcattga gatggaggat
    27721 aaaacaaaag cacaactcaa aagcaacttt cttaatggat tatcaccgtt tgctaacccc
    27781 aatgtggcaa atgctactga tggaaatgat tttgatttgc ggcaggtgcg gaaaaagcgg
    27841 atgacaattt atttctgtat ttctggtgat aatgccaggt tggctgaaaa aataacaaat
    27901 attttctttc agcttgctat acaggtgaat cttgaaaaga tgccaacgga tgatccggaa
    27961 ataaagcatg actgcctttt tctactggat gaattcccgt caattggtgc tgtagattac
    28021 atcaagagta aatctggtct cattgctggt tacaagctga aattgttgat tgtttaccag
    28081 gtcggctctc agcttgagga aatctacagt tatgcaggca gtaaaacatt actggcaagt
    28141 gcaccctgca aaattgttta ttccgcttct gatgttaaag atgcgcggga gttatcagaa
    28201 gcgatgggga cacgaacagt aactatcggt tcgaaaagca aaagtcgtag tcgtggagga
    28261 acatcgagat ctgaatcaga aagccttatt gagcgtccac tggtaacaac aaatgaacta
    28321 cttacactga aatttagtga agaaatcctg gcaatgaagg gagaaaaccc gattcgttgt
    28381 gaaaaggcat tctactatct caatgagtat ttctttggtg actttgtgaa ggttgctccg
    28441 gaacttgcaa acattttccc caggaagaaa ggaaagctgg ttatgcctcc acagaaagta
    28501 tttgaaaatg aaatcgtcgc aaaagggtat ctggtagtaa aagatgttcc tgatctcgat
    28561 aaagcggcat aaaggagaac atgatgaaga agttagtgtt tttattgatt tccattcttg
    28621 ctggttgttc atcaccgcca gagccaacac ccgtgcagtt tgaaaaggca aatgaagtga
    28681 tcaatccctc attgccatat gtccccgatt ttcacggtgt cataaagtca gacgttagcg
    28741 gtaaaggatg ggtttatgag atcacttcct tatccggagt acaggccaga acgccgacgt
    28801 tttattacgc tctggctcat gcggacagga ttgtagtgac aacacatgat gctgggctgt
    28861 ggtttcgaat cagagatctg ttgaagatgg aaggggcaac agcagtcgtg gaatggcgaa
    28921 acgagaaaag ttttttacct gaacaggtaa aaatagtttt cattaaagca caaaatgagg
    28981 tgaaaaatga ctggaaaaaa tagagtggtt ttaccagcct tgctggcgtt aggattaata
    29041 tcaacaaatg ctaatgcatc tgacccttgc gcttcggtac tttgcttata tggaaaagca
    29101 gtcgggcaag gtggagggag tgaatgcaga agtgctgaaa aggatttttt taatattctg
    29161 aaaaagaaaa aaggaagcat tcgctggagt aaaacttttg atgcccgtaa agcatttctg
    29221 aatcagtgtt caacggctga tcctgctgct atatcaaaaa tcatgagtaa gtttggtcgt
    29281 gtcagaggtt aattaaaacc tgataaccgc gccaacgggt atcagggtac tgcaagtatc
    29341 acatttcgcg ccaacgaaat gtgatgatta gttacaatca ctgaggttag aaatgaaagt
    29401 aaccaaatct attatagcta ttttattgtg cttatctgct ggtagtgcat aagtggatgt
    29461 aataaaaaat gctcgtgcct ttttgaaagg aaccctcgat ctgagcgttc aggaagcaga
    29521 tgaagatata gaggaaaggg aagaacttta taaaaagaac ggggcacaac ctgactattt
    29581 aagttacctg gaggattaac acatgcaata tgcattgttt gatggatttg aacgaaagtt
    29641 tttgctggat gctcttgaat tcggtgttct gaaggactgg aaagaaaatc cggtaaaaga
    29701 acttcctgat attgatgaat ctgctcaccc cttccatatc tgttatggtg gatatttatt
    29761 aaaccctggt gtttcagatt cagatattag cagaaaaata aaagaccagg caggattctg
    29821 gctggcagct attgatgata cccgcatgga ttgtcattca atagcttatt atgatattca
    29881 cacactccct ctgatttcgt gtggtcatca aaagatagtt ccttttgccg cgttaataaa
    29941 agctgatgaa tgcatcattt caaaaattgc ttcgtattct ggttttgctg taacagcctt
    30001 tttgagaatt aaagagtggg atattgcaac taatatactt aaccgtgagg gaatttttgc
    30061 ctttaatggc tgtgagcgca gattcagagt agtaagtaaa gataactggc aacatacagt
    30121 atcggaagaa cgcgctatcc gttgtgccaa aagattaatt caatgtaaag gataacaaaa
    30181 tgagactttt tatcgcagaa aaacccgcag tagcaaatga tattgttaag gcacttggtg
    30241 gcaattttac ccgccatgat ggctggtttg aaagtgataa cgccattgtg actaactgtt
    30301 ttggtcatat tatcgaatca caaccgccgg aaaactataa tcctgaatac aaagcctgga
    30361 aggttgaaac gcttccttta cgtctttatc ccgtgaagta tcagcctgtt gaaagtgcag
    30421 caaaacaggt taaaacgatt ctcgaactta tcagacgtgg agacgtgact gaaattgttc
    30481 acgctggcga tcctgatgat gagggacagc tactggttga tgaagtcctg gaatatgcag
    30541 gcaacacaaa acccgtaaag cgcgttctga ttaacgacaa cacgcttccg gcagtgaaaa
    30601 aggcactggc aaatcttaaa gacaatcgtg atttcaaagg actttacctt aaggcgttgg
    30661 cgcggtcagt tgccgatgcc gtctatggct tctctatgac acgtgcgtac accattccgg
    30721 caaaagccag aggatatcag ggcgttctgt ctgtcgggcg cgtccagact cccgttcttg
    30781 gcctgattgt gaatcgtacc cgtgctaacc agaaccataa atccagtttt tactacacca
    30841 tgaccggagt ctttcagcgt ggtgctgatg ttatcagggc gaactggaag ccaggggaat
    30901 ttgctccgct gacagaccgt aaattacttg ataaagcgtg ggcagacgga acggctgcat
    30961 cccttgcagg aaaaccggct acagttgaag cagcagcaac tgatgataaa aaaacggctg
    31021 cgccgttgcc gtttaatctg gtcagactcc agcaatacat gaacaagaag tttaaaatga
    31081 cggcacaaaa aacgctggat attacgcaac aactacgcga aaaatataaa gcaattacgt
    31141 ataaccgctc tgactgctca tatctttcag atgaacaatt cagcgaagcg ccgcaggtta
    31201 tcgatgccct gaaatcagtc tttcctcagt cgctggatat tgattctgca cgtaaaagca
    31261 aagcgtttaa cagtgcaaag gtgactgcgc atactgcgat aatcccgacc gccagtgtgc
    31321 ctgatgttaa cgcactcagc accgacgagc gcaatgttta cctggcgatc gcacaacact
    31381 atcttgttca gttcatgcct gaaaaagcat accaggaagt atcggttgcc attcagtgtg
    31441 gtgatgagtc gttctatgcc cgtgccagaa aaacaactga cagcggattt gaggcgtttc
    31501 ttggcgcgga aaccacagac gaaggtgaat cagaagataa tgatgattcc gcttttgaac
    31561 tgctctgtaa aattcgcaca ggagaaacac tgacgacaaa agaagttatt gttaatgaga
    31621 agaaaacaac accgccgccg ttattcaccg aagcctcctt gcttgctgcg cttgttcgtg
    31681 tcgcggattt tgtcactgat ccaacaatta aaaaattgtt gaaggataag gataaagaca
    31741 aaaaagatga acatggtggc attggtacgc cagctacccg cgcagccatt ctggaaacgc
    31801 tgaagaaaag aaactatatc acgctggaaa aagggaaact tattcctact gataccggat
    31861 atgcgcttat tgatgccctg cctggtatag cggttaatcc tgatatgaca gcattatggt
    31921 ctgaaaagca ggctgccata gaaaatggcg acctgacggt tgaacagttt attaatgatc
    31981 tgtacggtga actgacaggc atgatttctg atgttgacct gggcgagatg aagattgaac
    32041 ccgctgcgcc agcagggcag tttcaacgcc tggactctcc ctgcccttcc tgtggtaaac
    32101 atattgttat caggccgaaa ggttatttct gtaccggatg tgaatttaaa atctggagtg
    32161 agttttctgg taagaaaatc acccaggcac aggccgaaaa actggttaaa tcagggaaaa
    32221 ccgatttgat taagggattt aaaaagaaaa gtggtggaac gtatgataca gttcttgtcc
    32281 ttgaggataa gaaaacaggg aagctgggtt ttccggcaag ggctaagaag tgaaaacaaa
    32341 gcaggaatgg ctttttcagt taagaaaatg tacatcaaga gatactcttg aaaaagttat
    32401 tgagattaac cgttacaaac tgcctttatc agaatcagag gcattttatt ctgccgcaga
    32461 tcatcgccga gcagaactgg tgatgaataa actttatgat aaggtgcctt ccggcgtctg
    32521 gaagtacgta cactaaataa gaggattaat tatatgagcg aactgactaa agaagatgaa
    32581 tacggcatta tcagccgtac tatgatgaat attcgttcat tgcgtgtttt tgcccgtgag
    32641 attgattttg agcagttgct cgaaatgcag gaaaagctca acgttgttat tgaagaacgt
    32701 cgtgaagatg ctgaacgtga agcggctgaa cgccaggaac ttgaagccaa acgtcagcag
    32761 gccattgaat acatcatatc tttaggtctt gatcccgaat cactccttgc tcctgtaaca
    32821 gcagatacag taaaaaccag gcggaaagcc aaaggcggag taagaaaggc taaataccgc
    32881 tttaaggatg aaaatggcga gatcagagaa tggtctggta atggcaagcg tccgcttgct
    32941 ttacagaagc tgcttgatga ggggcatttc atggaagatt tcctcattga gaaaactaag
    33001 ccggaacagg cagaataacc cgccaagtat acatcacaca gggcactgtt gcaaatagtc
    33061 ggtggtgata aacttatcat ccccttttgc tgatggagct gcacatgaac ccattcaaag
    33121 gccggcattt tcagcgtgac atcattctgt gggccgtacg ctggtactgc aaatacggca
    33181 tcagttaccg tgagctgcag gagatgctgg ctgaacgcgg agtgaatgtc gatcactcca
    33241 cgatttaccg ctgggttcag cgttatgcgc ctgaaatgga aaaacggctg cgctggtact
    33301 ggcgtaaccc ttccgatctt tgcccgtggc acatggatga aacctacgtg aaggtcaatg
    33361 gccgctgggc gtatctgtac cgggccgtcg acagccgggg ccgcactgtc gatttttatc
    33421 tctcctcccg tcgtaacagc aaagctgcat accggtttct gggtaaaatc ctcaacaacg
    33481 tgaagaagtg gcagatcccg cgattcatca acacggataa agcgcccgcc tatggtcgcg
    33541 cgcttgctct gctcaaacgc gaaggccggt gcccgtctga cgttgaacac cgacagatta
    33601 agtaccggaa caacgtgatt gaatgcgatc atggcaaact gaaacggata atcggcgcca
    33661 cgctgggatt taaatccatg aagacggctt acgccaccat caaaggtatt gaggtgatgc
    33721 gtgcactacg caaaggccag gcctcagcat tttattatgg tgatcccctg ggcgaaatgc
    33781 gcctggtaag cagagttttt gaaatgtaag gcctttgaat aagacaaaag gctgcctcat
    33841 cgctaacttt gcaacagtgc ctcacacaga catacacata ggtatgtctg tgtgacatac
    33901 ctatgtacag tgccagatgg cgttgctacg tgaaaaataa cctaccctac ccctgctttt
    33961 ttcgcagatt ttacgtagca caccgttgct atagcattca tctaaaaagc tgaatgtcct
    34021 tttctgtcgc tgttgcattt taattcactg aaaaatattg agtaatgaaa atgccggtaa
    34081 cgcgtcaagc tgttggccaa cgcgacaggc agtattggaa gcaacaaaaa aaatgaaaga
    34141 aaaatccctc aaatgaagga gaaaaaacgg aactacagcc gaacgacagg cacaaaaaaa
    34201 cccgactagt aatcgggctt ttttgcgttt tccagtcttg agttggtggc tctgaccgga
    34261 gaaggttact ctacacctaa cgtttacatt ttattcacta tggtgctgta aagcaactaa
    34321 atgtggttaa ggaaccacat ttagtggtgt aacgggtgtt ttgtgtagcg tataatcagt
    34381 cagttagtca tgtagttggc ttgcaatcct gctgttcccg tatctgcggg tatgaacgat
    34441 tttaaagtcc tggctggata aacaggtggt gctgtattcg gtgctctggc aggaggaggt
    34501 cactctacac ctaacaatgc gagagagccg ttgtgacagg cagtgtatga gcaagcaacg
    34561 gtgatttcct ccagttttac tggagaaacg gatgattaat atgttaatca tcgttctcag
    34621 agctgtggtc gccgttgcaa acgcg
//
